#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q9BPX6	uniprotkb:Q9BPX6	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Wang et al. (2014)	imex:IM-23081|pubmed:24514027	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9550902|imex:IM-23081-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	rcsb pdb:4NSC|rcsb pdb:4NSD	-	-	figure legend:f1 f2c t1 sf4|comment:"Consistent with the observations in solution, the Ca2+-free  MICU1-xtal crystal structure revealed six molecules in the asymmetric  unit, which are packed by a trimer of dimers (Fig 2C and Supplementary  Fig 4). Unexpectedly, in the Ca2+-free MICU1-xtal  structure, the C-helix is located in the center of the six molecules  that are packed as a helix bundle."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/17	2014/10/16	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	intact-crc:9AC5EBFFDC0A0047|rigid:VCsOFDNcU0n2cwais1HMsPTAn08	false	binding-associated region:97-476	binding-associated region:97-476	6	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q9BPX6	uniprotkb:Q9BPX6	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Wang et al. (2014)	imex:IM-23081|pubmed:24514027	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9550977|imex:IM-23081-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	rcsb pdb:4NSC|rcsb pdb:4NSD	-	-	figure legend:f3 t1|comment:"Ca2+ binding to MICU1 induced large conformational changes in the monomer, which led to a completely different dimer formation. The interface of the Ca2+-bound MICU1-xtal-deltaC dimer was composed of the CH4 helix from both monomers as well as the CH4-CH5 loop from one monomer with the NH3 helix from the reciprocal monomer. The buried surface area of the Ca2+-bound MICU1-xtal-deltaC dimer was approximately 434 A2 (Fig 3B). The overall dimers of the Ca2+-free and the Ca2+-bound MICU1 structures could not be superposed. After the superposition of each monomer from the two dimers, the additional superposition of the second monomer from the two dimers required an approximately 74° rotation (Fig 3C)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/17	2014/10/16	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	intact-crc:B28E972F9C80DAA9|rigid:VCsOFDNcU0n2cwais1HMsPTAn08	false	binding-associated region:97-444	binding-associated region:97-444	2	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q9BPX6	uniprotkb:Q9BPX6	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:"MI:0028"(cosedimentation in solution)	Wang et al. (2014)	imex:IM-23081|pubmed:24514027	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9551019|imex:IM-23081-6	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	-	-	-	figure legend:sf3c  f2e f2f f3f f3g|comment:"To verify this hypothesis, we determined  the oligomerization state of MICU1-xtal-deltaC. This mutant  exhibited a predominant peak corresponding to the MICU1 dimer  both in the absence and in the presence of Ca2+ (Fig 2E, F and  Supplementary Fig 3C), suggesting that the C-helix is necessary for  the formation of the Ca2+-free MICU1-xtal hexamer and substantially  contributed to the formation of multiple oligomers of Ca2+-bound  MICU1-xtal."|comment:"Within the Ca2+-free MICU1-xtal dimer interface, the two monomers  primarily interact through a salt bridge. Asp376 from one monomer  interacts with Arg221 from the reciprocal monomer via a salt  bridge (Fig 3D). The R221A or D376A single mutation or the R221AD376A  double mutation of MICU1-xtal-deltaC converted the dimer  into the monomer in the absence of Ca2+ (Fig 3F). The Ca2+-bound  MICU1-xtal-deltaC dimer interface is formed by hydrogen bonding  and hydrophobic interactions (Fig 3E)"|comment:"The R221A single mutation of MICU1-  xtal-deltaC was unable to disrupt the dimer formation in the presence  of Ca2+. The F383A-H385A double mutation of MICU1-xtal-deltaC  converted the dimer into the monomer, and the dimer mixture had  an approximate ratio of 1:1 in the presence of Ca2+ (Fig 3G)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/17	2014/10/16	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	intact-crc:A636C0C4F137F66A|rigid:VCsOFDNcU0n2cwais1HMsPTAn08	false	binding-associated region:97-444|mutation disrupting interaction:221-221,376-376	binding-associated region:97-444|mutation disrupting interaction:221-221,376-376	2	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q9BPX6	uniprotkb:Q9BPX6	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:"MI:0028"(cosedimentation in solution)	Wang et al. (2014)	imex:IM-23081|pubmed:24514027	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9551030|imex:IM-23081-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	-	-	-	figure legend:sf3c  f2e f2f f3f f3g|comment:"To verify this hypothesis, we determined  the oligomerization state of MICU1-xtal-deltaC. This mutant  exhibited a predominant peak corresponding to the MICU1 dimer  both in the absence and in the presence of Ca2+ (Fig 2E, F and  Supplementary Fig 3C), suggesting that the C-helix is necessary for  the formation of the Ca2+-free MICU1-xtal hexamer and substantially  contributed to the formation of multiple oligomers of Ca2+-bound  MICU1-xtal."|comment:"Within the Ca2+-free MICU1-xtal dimer interface, the two monomers  primarily interact through a salt bridge. Asp376 from one monomer  interacts with Arg221 from the reciprocal monomer via a salt  bridge (Fig 3D). The R221A or D376A single mutation or the R221AD376A  double mutation of MICU1-xtal-deltaC converted the dimer  into the monomer in the absence of Ca2+ (Fig 3F). The Ca2+-bound  MICU1-xtal-deltaC dimer interface is formed by hydrogen bonding  and hydrophobic interactions (Fig 3E)"|comment:"The R221A single mutation of MICU1-  xtal-deltaC was unable to disrupt the dimer formation in the presence  of Ca2+. The F383A-H385A double mutation of MICU1-xtal-deltaC  converted the dimer into the monomer, and the dimer mixture had  an approximate ratio of 1:1 in the presence of Ca2+ (Fig 3G)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/17	2014/10/16	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	intact-crc:A5A32DF45F34614A|rigid:VCsOFDNcU0n2cwais1HMsPTAn08	false	mutation disrupting interaction:376-376|binding-associated region:97-444	mutation disrupting interaction:376-376|binding-associated region:97-444	2	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q9BPX6	uniprotkb:Q9BPX6	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:"MI:0028"(cosedimentation in solution)	Wang et al. (2014)	imex:IM-23081|pubmed:24514027	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9550961|imex:IM-23081-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	-	-	-	figure legend:sf3c  f2e f2f f3f f3g|comment:"To verify this hypothesis, we determined  the oligomerization state of MICU1-xtal-deltaC. This mutant  exhibited a predominant peak corresponding to the MICU1 dimer  both in the absence and in the presence of Ca2+ (Fig 2E, F and  Supplementary Fig 3C), suggesting that the C-helix is necessary for  the formation of the Ca2+-free MICU1-xtal hexamer and substantially  contributed to the formation of multiple oligomers of Ca2+-bound  MICU1-xtal."|comment:"Within the Ca2+-free MICU1-xtal dimer interface, the two monomers  primarily interact through a salt bridge. Asp376 from one monomer  interacts with Arg221 from the reciprocal monomer via a salt  bridge (Fig 3D). The R221A or D376A single mutation or the R221AD376A  double mutation of MICU1-xtal-deltaC converted the dimer  into the monomer in the absence of Ca2+ (Fig 3F). The Ca2+-bound  MICU1-xtal-deltaC dimer interface is formed by hydrogen bonding  and hydrophobic interactions (Fig 3E)"|comment:"The R221A single mutation of MICU1-  xtal-deltaC was unable to disrupt the dimer formation in the presence  of Ca2+. The F383A-H385A double mutation of MICU1-xtal-deltaC  converted the dimer into the monomer, and the dimer mixture had  an approximate ratio of 1:1 in the presence of Ca2+ (Fig 3G)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/17	2014/10/16	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	intact-crc:B11A14A0A4B6EF9F|rigid:VCsOFDNcU0n2cwais1HMsPTAn08	false	mutation disrupting interaction:221-221|binding-associated region:97-444	mutation disrupting interaction:221-221|binding-associated region:97-444	2	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q9BPX6	uniprotkb:Q9BPX6	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:"MI:0028"(cosedimentation in solution)	Wang et al. (2014)	imex:IM-23081|pubmed:24514027	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9550934|imex:IM-23081-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	-	-	-	figure legend:sf3a f2a|comment:"In the absence of Ca2+, MICU1-xtal in solution predominantly  existed as a hexamer (~237 kDa, theoretical molecular weight of  43 kDa for each molecule) (Fig 2A and Supplementary Fig 3A),  whereas, in the presence of Ca2+, MICU1-xtal existed as a mixture  of oligomeric forms (Fig 2B and Supplementary Fig 3B)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/17	2014/10/16	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	intact-crc:C33A194E8F72AA27|rigid:VCsOFDNcU0n2cwais1HMsPTAn08	false	binding-associated region:97-476	binding-associated region:97-476	6	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q9BPX6	uniprotkb:Q9BPX6	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:"MI:0028"(cosedimentation in solution)	Wang et al. (2014)	imex:IM-23081|pubmed:24514027	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9551041|imex:IM-23081-8	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	-	-	-	figure legend:sf3c  f2e f2f f3f f3g|comment:"To verify this hypothesis, we determined  the oligomerization state of MICU1-xtal-deltaC. This mutant  exhibited a predominant peak corresponding to the MICU1 dimer  both in the absence and in the presence of Ca2+ (Fig 2E, F and  Supplementary Fig 3C), suggesting that the C-helix is necessary for  the formation of the Ca2+-free MICU1-xtal hexamer and substantially  contributed to the formation of multiple oligomers of Ca2+-bound  MICU1-xtal."|comment:"Within the Ca2+-free MICU1-xtal dimer interface, the two monomers  primarily interact through a salt bridge. Asp376 from one monomer  interacts with Arg221 from the reciprocal monomer via a salt  bridge (Fig 3D). The R221A or D376A single mutation or the R221AD376A  double mutation of MICU1-xtal-deltaC converted the dimer  into the monomer in the absence of Ca2+ (Fig 3F). The Ca2+-bound  MICU1-xtal-deltaC dimer interface is formed by hydrogen bonding  and hydrophobic interactions (Fig 3E)"|comment:"The R221A single mutation of MICU1-  xtal-deltaC was unable to disrupt the dimer formation in the presence  of Ca2+. The F383A-H385A double mutation of MICU1-xtal-deltaC  converted the dimer into the monomer, and the dimer mixture had  an approximate ratio of 1:1 in the presence of Ca2+ (Fig 3G)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/17	2014/10/16	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	intact-crc:13F6BD06A24FFA5D|rigid:VCsOFDNcU0n2cwais1HMsPTAn08	false	binding-associated region:97-444|mutation disrupting interaction:383-383,385-385	binding-associated region:97-444|mutation disrupting interaction:383-383,385-385	2	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q9BPX6	uniprotkb:Q9BPX6	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	intact:EBI-2371996|uniprotkb:O75785|uniprotkb:Q9H9N6|uniprotkb:Q9UFX0|uniprotkb:A8MV96|uniprotkb:B3KN20|uniprotkb:B4DJH9|uniprotkb:B4DPI1|uniprotkb:B5MBY3|uniprotkb:D3YTJ3|ensembl:ENSP00000354415	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:micu1_human(display_long)|uniprotkb:MICU1(gene name)|psi-mi:MICU1(display_short)|uniprotkb:Calcium-binding atopy-related autoantigen 1(gene name synonym)|uniprotkb:CBARA1(gene name synonym)|uniprotkb:CALC(gene name synonym)|uniprotkb:Atopy-related autoantigen CALC(gene name synonym)	psi-mi:"MI:0028"(cosedimentation in solution)	Wang et al. (2014)	imex:IM-23081|pubmed:24514027	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9550952|imex:IM-23081-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	refseq:NP_001182447.1|refseq:NP_001182448.1|refseq:NP_006068.2|dip:DIP-53438N|ensembl:ENSG00000107745(gene)|ensembl:ENST00000361114(transcript)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005622"(intracellular anatomical structure)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006851"(mitochondrial calcium ion transmembrane transport)|go:"GO:0006952"(defense response)|go:"GO:0032592"(integral component of mitochondrial membrane)|go:"GO:0034704"(calcium channel complex)|go:"GO:0036444"(calcium import into the mitochondrion)|go:"GO:0042802"(identical protein binding)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051260"(protein homooligomerization)|go:"GO:0051560"(mitochondrial calcium ion homeostasis)|go:"GO:0051561"(positive regulation of mitochondrial calcium ion concentration)|go:"GO:0070509"(calcium ion import)|go:"GO:1900069"(regulation of cellular hyperosmotic salinity response)|go:"GO:1990246"(uniplex complex)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR039800|rcsb pdb:4NSC|rcsb pdb:4NSD|rcsb pdb:6K7Y|rcsb pdb:6LB7|rcsb pdb:6LB8|rcsb pdb:6LE5|rcsb pdb:6WDN|rcsb pdb:6WDO|rcsb pdb:6XJX|rcsb pdb:6XQN|rcsb pdb:6XQO|reactome:R-HSA-8949215|reactome:R-HSA-8949664|rcsb pdb:6XJV	-	-	-	figure legend:sf3b f2b|comment:"In the absence of Ca2+, MICU1-xtal in solution predominantly  existed as a hexamer (~237 kDa, theoretical molecular weight of  43 kDa for each molecule) (Fig 2A and Supplementary Fig 3A),  whereas, in the presence of Ca2+, MICU1-xtal existed as a mixture  of oligomeric forms (Fig 2B and Supplementary Fig 3B)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/17	2014/10/16	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	rogid:ieoktRyh6dR8RvLctKOhbT84qPU9606	intact-crc:1E67BE63E41C1F82|rigid:T3i0UKMVCitrxfmtwWBbY/GDO/M	false	binding-associated region:97-476	binding-associated region:97-476	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)