#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:O08561 uniprotkb:P14562 intact:EBI-9085347|ensembl:ENSRNOP00000039342 intact:EBI-916562|uniprotkb:P97620|ensembl:ENSRNOP00000026580 psi-mi:pi4kb_rat(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:lamp1_rat(display_long)|uniprotkb:120 kDa lysosomal membrane glycoprotein(gene name synonym)|uniprotkb:CD107 antigen-like family member A(gene name synonym)|uniprotkb:Lamp1(gene name)|psi-mi:Lamp1(display_short)|uniprotkb:Lamp-1(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085400|imex:IM-22093-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621214|refseq:NP_112345.1|ensembl:ENSRNOG00000021024(gene)|ensembl:ENSRNOT00000040501(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-RNO-1660514 rgd:2989|refseq:NP_036989.1|ensembl:ENSRNOG00000019629(gene)|ensembl:ENSRNOT00000026580(transcript)|go:"GO:0000421"(autophagosome membrane)|go:"GO:0005737"(cytoplasm)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005768"(endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005771"(multivesicular body)|go:"GO:0005773"(vacuole)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0010008"(endosome membrane)|go:"GO:0019899"(enzyme binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030425"(dendrite)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031902"(late endosome membrane)|go:"GO:0031982"(vesicle)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0044194"(cytolytic granule)|go:"GO:0044754"(autolysosome)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048102"(autophagic cell death)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050821"(protein stabilization)|go:"GO:0061474"(phagolysosome membrane)|go:"GO:0072594"(establishment of protein localization to organelle)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0097208"(alveolar lamellar body)|go:"GO:0101004"(cytolytic granule membrane)|go:"GO:0140507"(granzyme-mediated programmed cell death signaling pathway)|go:"GO:1902513"(regulation of organelle transport along microtubule)|interpro:IPR002000("Lysosome-associated membrane glycoprotein (Lamp)/CD68")|interpro:IPR018134|reactome:R-RNO-6798695|refseq:XP_017455483.1 - - - figure legend:f6g|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/01/15 2014/10/16 rogid:yTl5CFz9dSADvKpr1FLFVoTg6mA10116 rogid:2mCfYIdewLVD+Tur5waxSU9muhE10116 intact-crc:9A72DD80CED7694F|rigid:IOgRLJ0V1tnYSluF8hLrcuPQlzE false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O08561 uniprotkb:Q70AM4 intact:EBI-9085347|ensembl:ENSRNOP00000039342 intact:EBI-9085372|uniprotkb:F1LRB4|ensembl:ENSRNOP00000032499 psi-mi:pi4kb_rat(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:q70am4_rat(display_long)|uniprotkb:Kif13b(gene name)|psi-mi:Kif13b(display_short)|uniprotkb:kif13B(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085400|imex:IM-22093-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621214|refseq:NP_112345.1|ensembl:ENSRNOG00000021024(gene)|ensembl:ENSRNOT00000040501(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-RNO-1660514 rgd:1303307|refseq:NP_998791.1|ensembl:ENSRNOG00000013089(gene)|ensembl:ENSRNOT00000029757(transcript)|go:"GO:0003777"(microtubule motor activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005871"(kinesin complex)|go:"GO:0005874"(microtubule)|go:"GO:0007018"(microtubule-based movement)|go:"GO:0008017"(microtubule binding)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030424"(axon)|go:"GO:0050770"(regulation of axonogenesis)|go:"GO:0071889"(14-3-3 protein binding)|go:"GO:1990939"|interpro:IPR000253(Forkhead-associated)|interpro:IPR000938(Cytoskeleton-associated protein, CAP-Gly)|interpro:IPR001752(Kinesin, motor region)|interpro:IPR008984(SMAD/FHA domain)|interpro:IPR019821|interpro:IPR022140|interpro:IPR022164|interpro:IPR027417|interpro:IPR032405|interpro:IPR036859|interpro:IPR036961 - - - figure legend:f6g|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/01/15 2014/10/16 rogid:yTl5CFz9dSADvKpr1FLFVoTg6mA10116 rogid:T29aXhCTGC+xgmQOo2Pcsh0ECUU10116 intact-crc:9A72DD80CED7694F|rigid:IOgRLJ0V1tnYSluF8hLrcuPQlzE false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q8BKC8 uniprotkb:P06797 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-7876993|uniprotkb:Q91UZ0|intact:MINT-1863740|ensembl:ENSMUSP00000021933|ensembl:ENSMUSP00000152169 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:catl1_mouse(display_long)|uniprotkb:Ctsl(gene name)|psi-mi:Ctsl(display_short)|uniprotkb:Ctsl1(gene name synonym)|uniprotkb:Major excreted protein(gene name synonym)|uniprotkb:p39 cysteine proteinase(gene name synonym)|uniprotkb:Cathepsin L1(gene name synonym) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_034114.1|refseq:XP_006517143.1|go:"GO:0043373"(CD4-positive, alpha-beta T cell lineage commitment)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0046697"(decidualization)|go:"GO:0048002"(antigen processing and presentation of peptide antigen)|go:"GO:0048102"(autophagic cell death)|go:"GO:0051384"(response to glucocorticoid)|go:"GO:0051603"(proteolysis involved in cellular protein catabolic process)|go:"GO:0060008"(Sertoli cell differentiation)|go:"GO:0060309"(elastin catabolic process)|go:"GO:0062023"(collagen-containing extracellular matrix)|interpro:IPR000169(Peptidase, cysteine peptidase active site)|interpro:IPR000668(Peptidase C1A, papain C-terminal)|interpro:IPR013201(Proteinase inhibitor I29, cathepsin propeptide)|go:"GO:0043204"(perikaryon)|interpro:IPR025660|interpro:IPR025661|interpro:IPR038765|interpro:IPR039417|rcsb pdb:1MVV|reactome:R-MMU-1442490|reactome:R-MMU-1474228|reactome:R-MMU-1679131|reactome:R-MMU-2022090|reactome:R-MMU-2132295|reactome:R-MMU-8939242|ensembl:ENSMUSG00000021477(gene)|ensembl:ENSMUST00000021933(transcript)|ensembl:ENSMUST00000222517(transcript)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004177"(aminopeptidase activity)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005634"(nucleus)|go:"GO:0005730"(nucleolus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005764"(lysosome)|go:"GO:0005773"(vacuole)|go:"GO:0005902"(microvillus)|go:"GO:0006508"(proteolysis)|go:"GO:0006590"(thyroid hormone generation)|go:"GO:0006955"(immune response)|go:"GO:0007154"(cell communication)|go:"GO:0007283"(spermatogenesis)|go:"GO:0008584"(male gonad development)|go:"GO:0009749"(response to glucose)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0010259"(multicellular organism aging)|go:"GO:0010839"(negative regulation of keratinocyte proliferation)|go:"GO:0014070"(response to organic cyclic compound)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0016807"(cysteine-type carboxypeptidase activity)|go:"GO:0021675"(nerve development)|go:"GO:0030574"(collagen catabolic process)|go:"GO:0030984"(kininogen binding)|go:"GO:0031069"(hair follicle morphogenesis)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031638"(zymogen activation)|go:"GO:0034230"(enkephalin processing)|go:"GO:0034698"(response to gonadotropin)|go:"GO:0042277"(peptide binding)|go:"GO:0042393"(histone binding)|go:"GO:0042583"(chromaffin granule)|go:"GO:0009267"(cellular response to starvation)|go:"GO:0043005"(neuron projection) go:"GO:0033107"(Cvt vesicle) - comment:mint|function:Important for the overall degradation of proteins in lysosomes figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:oFAYLk0ZhXto2nbmx/lUdQc2EJM10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:P51150 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-8321380|intact:MINT-1864693|ensembl:ENSMUSP00000092658|ensembl:ENSMUSP00000109226|ensembl:ENSMUSP00000109227|ensembl:ENSMUSP00000109228|ensembl:ENSMUSP00000109230 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:rab7a_mouse(display_long)|uniprotkb:Rab7a(gene name)|psi-mi:Rab7a(display_short)|uniprotkb:Rab7(gene name synonym) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_001280581.1|refseq:NP_001280582.1|refseq:NP_001280583.1|refseq:NP_001280584.1|refseq:NP_033031.2|dip:DIP-60513N|reactome:R-MMU-9013149|reactome:R-MMU-9013404|reactome:R-MMU-9013405|reactome:R-MMU-9013406|reactome:R-MMU-9013407|reactome:R-MMU-9013408|reactome:R-MMU-9013423|reactome:R-MMU-9035034|reactome:R-MMU-9013148|ensembl:ENSMUSG00000079477(gene)|ensembl:ENSMUST00000095048(transcript)|ensembl:ENSMUST00000113596(transcript)|ensembl:ENSMUST00000113597(transcript)|ensembl:ENSMUST00000113598(transcript)|ensembl:ENSMUST00000113600(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0000421"(autophagosome membrane)|go:"GO:0003924"(GTPase activity)|go:"GO:0003925"(G protein activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005764"(lysosome)|go:"GO:0005768"(endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006622"(protein targeting to lysosome)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0007174"(epidermal growth factor catabolic process)|go:"GO:0008333"(endosome to lysosome transport)|go:"GO:0010008"(endosome membrane)|go:"GO:0016042"(lipid catabolic process)|go:"GO:0019003"(GDP binding)|go:"GO:0019076"(viral release from host cell)|go:"GO:0022615"(protein to membrane docking)|go:"GO:0030670"(phagocytic vesicle membrane)|go:"GO:0031267"(small GTPase binding)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031902"(late endosome membrane)|go:"GO:0032419"(extrinsic component of lysosome membrane)|go:"GO:0033162"(melanosome membrane)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0045453"(bone resorption)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0048524"(positive regulation of viral process)|go:"GO:0051650"(establishment of vesicle localization)|go:"GO:0061724"(lipophagy)|go:"GO:0090383"(phagosome acidification)|go:"GO:0090385"(phagosome-lysosome fusion)|go:"GO:0097208"(alveolar lamellar body)|go:"GO:0098993"(anchored component of synaptic vesicle membrane)|go:"GO:0099638"(endosome to plasma membrane protein transport)|go:"GO:1903542"(negative regulation of exosomal secretion)|go:"GO:1903543"(positive regulation of exosomal secretion)|go:"GO:1905394"(retromer complex binding)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR027417|mint:P51150|rcsb pdb:5Z2M|reactome:R-MMU-2132295|reactome:R-MMU-6798695|reactome:R-MMU-8854214|reactome:R-MMU-8873719|reactome:R-MMU-8876198 go:"GO:0033107"(Cvt vesicle) - comment:mint|function:"Involved in late endocytic transport. Contributes to the maturation of phagosomes (acidification)" figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:AJbKizzw4zCAn+roRF2Im1Tt4xE10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:Q68FD5 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-769168|ensembl:ENSMUSP00000099475 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:clh1_mouse(display_long)|uniprotkb:Cltc(gene name)|psi-mi:Cltc(display_short) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_001003908.1|dip:DIP-31947N|ensembl:ENSMUSG00000047126(gene)|ensembl:ENSMUST00000103186(transcript)|go:"GO:0000278"(mitotic cell cycle)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0005198"(structural molecule activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0005768"(endosome)|go:"GO:0005819"(spindle)|go:"GO:0005829"(cytosol)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006914"(autophagy)|go:"GO:0007030"(Golgi organization)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016020"(membrane)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030117"(membrane coat)|go:"GO:0030118"(clathrin coat)|go:"GO:0030130"(clathrin coat of trans-Golgi network vesicle)|go:"GO:0030132"(clathrin coat of coated pit)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030315"(T-tubule)|go:"GO:0030506"(ankyrin binding)|go:"GO:0031072"(heat shock protein binding)|go:"GO:0031523"(Myb complex)|go:"GO:0031623"(receptor internalization)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033572"(transferrin transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0042277"(peptide binding)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043209"(myelin sheath)|go:"GO:0045334"(clathrin-coated endocytic vesicle)|go:"GO:0048268"(clathrin coat assembly)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0050750"(low-density lipoprotein particle receptor binding)|go:"GO:0051301"(cell division)|go:"GO:0060236"(regulation of mitotic spindle organization)|go:"GO:0071439"(clathrin complex)|go:"GO:0072583"(clathrin-dependent endocytosis)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090307"(mitotic spindle assembly)|go:"GO:0097718"(disordered domain specific binding)|go:"GO:0098684"(photoreceptor ribbon synapse)|go:"GO:0098835"(presynaptic endocytic zone membrane)|go:"GO:0098850"(extrinsic component of synaptic vesicle membrane)|go:"GO:0150093"(amyloid-beta clearance by transcytosis)|go:"GO:1900126"(negative regulation of hyaluronan biosynthetic process)|go:"GO:1903077"(negative regulation of protein localization to plasma membrane)|go:"GO:1990381"(ubiquitin-specific protease binding)|go:"GO:1990498"(mitotic spindle microtubule)|interpro:IPR000547(Clathrin, heavy chain/VPS, 7-fold repeat)|interpro:IPR011990(Tetratricopeptide-like helical)|interpro:IPR015348(Clathrin, heavy chain, linker, core motif)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR016025(Clathrin, heavy chain, linker and propeller)|interpro:IPR016341(Clathrin, heavy chain)|interpro:IPR022365|mint:Q68FD5|rcsb pdb:5ODS|reactome:R-MMU-177504|reactome:R-MMU-190873|reactome:R-MMU-196025|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|reactome:R-MMU-437239|reactome:R-MMU-5099900|reactome:R-MMU-5140745|reactome:R-MMU-8856825|reactome:R-MMU-8856828|reactome:R-MMU-8866427|reactome:R-MMU-8964038|reactome:R-MMU-9013420|reactome:R-MMU-9013424|go:"GO:0032051"(clathrin light chain binding) go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:iHg/TOG2rWePqtsEmuc/szn/chI10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:O08788 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-776180|uniprotkb:E9QLJ1|uniprotkb:Q3TZG7|ensembl:ENSMUSP00000109552 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:dctn1_mouse(display_long)|uniprotkb:150 kDa dynein-associated polypeptide(gene name synonym)|uniprotkb:DAP-150(gene name synonym)|uniprotkb:p150-glued(gene name synonym)|uniprotkb:Dctn1(gene name)|psi-mi:Dctn1(display_short) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_031861.2|dip:DIP-32057N|ensembl:ENSMUSG00000031865(gene)|ensembl:ENSMUST00000113919(transcript)|go:"GO:0000132"(establishment of mitotic spindle orientation)|go:"GO:0000776"(kinetochore)|go:"GO:0000922"(spindle pole)|go:"GO:0005634"(nucleus)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005737"(cytoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0005814"(centriole)|go:"GO:0005819"(spindle)|go:"GO:0005829"(cytosol)|go:"GO:0005868"(cytoplasmic dynein complex)|go:"GO:0005874"(microtubule)|go:"GO:0005875"(microtubule associated complex)|go:"GO:0005938"(cell cortex)|go:"GO:0007097"(nuclear migration)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0008017"(microtubule binding)|go:"GO:0010457"(centriole-centriole cohesion)|go:"GO:0015630"(microtubule cytoskeleton)|go:"GO:0015631"(tubulin binding)|go:"GO:0019901"(protein kinase binding)|go:"GO:0021517"(ventral spinal cord development)|go:"GO:0030424"(axon)|go:"GO:0031116"(positive regulation of microtubule polymerization)|go:"GO:0031122"(cytoplasmic microtubule organization)|go:"GO:0031252"(cell leading edge)|go:"GO:0032402"(melanosome transport)|go:"GO:0032991"(protein-containing complex)|go:"GO:0034454"(microtubule anchoring at centrosome)|go:"GO:0035371"(microtubule plus-end)|go:"GO:0036064"(ciliary basal body)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0042802"(identical protein binding)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0045171"(intercellular bridge)|go:"GO:0050905"(neuromuscular process)|go:"GO:0051010"(microtubule plus-end binding)|go:"GO:0051081"(nuclear membrane disassembly)|go:"GO:0051301"(cell division)|go:"GO:0060236"(regulation of mitotic spindle organization)|go:"GO:0061744"(motor behavior)|go:"GO:0070050"(neuron cellular homeostasis)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090063"(positive regulation of microtubule nucleation)|go:"GO:0090316"(positive regulation of intracellular protein transport)|go:"GO:0099738"(cell cortex region)|go:"GO:0120103"(centriolar subdistal appendage)|go:"GO:1904398"(positive regulation of neuromuscular junction development)|go:"GO:1905515"(non-motile cilium assembly)|go:"GO:1990535"(neuron projection maintenance)|interpro:IPR000938(Cytoskeleton-associated protein, CAP-Gly)|interpro:IPR022157|interpro:IPR036859|mint:O08788|reactome:R-MMU-2132295|reactome:R-MMU-2565942|reactome:R-MMU-3371497|reactome:R-MMU-380259|reactome:R-MMU-380270|reactome:R-MMU-380284|reactome:R-MMU-380320|reactome:R-MMU-5620912|reactome:R-MMU-6807878|reactome:R-MMU-6811436|reactome:R-MMU-8854518 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:pqj7TtNDgexEbxqLwksW3eW41bo10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:Q61768 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-776129|uniprotkb:Q5BL10|uniprotkb:O08711|uniprotkb:Q61580|ensembl:ENSMUSP00000025083 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:kinh_mouse(display_long)|uniprotkb:Ubiquitous kinesin heavy chain(gene name synonym)|uniprotkb:Conventional kinesin heavy chain(gene name synonym)|uniprotkb:Kif5b(gene name)|psi-mi:Kif5b(display_short)|uniprotkb:Khcs(gene name synonym)|uniprotkb:Kns1(gene name synonym) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_032474.2|dip:DIP-32053N|ensembl:ENSMUSG00000006740(gene)|ensembl:ENSMUST00000025083(transcript)|go:"GO:0003777"(microtubule motor activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005871"(kinesin complex)|go:"GO:0005874"(microtubule)|go:"GO:0006839"(mitochondrial transport)|go:"GO:0007017"(microtubule-based process)|go:"GO:0007018"(microtubule-based movement)|go:"GO:0007028"(cytoplasm organization)|go:"GO:0007411"(axon guidance)|go:"GO:0008017"(microtubule binding)|go:"GO:0008432"(JUN kinase binding)|go:"GO:0008574"(plus-end-directed microtubule motor activity)|go:"GO:0015630"(microtubule cytoskeleton)|go:"GO:0021766"(hippocampus development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030705"(cytoskeleton-dependent intracellular transport)|go:"GO:0031340"(positive regulation of vesicle fusion)|go:"GO:0031982"(vesicle)|go:"GO:0032230"(positive regulation of synaptic transmission, GABAergic)|go:"GO:0032418"(lysosome localization)|go:"GO:0032839"(dendrite cytoplasm)|go:"GO:0034451"(centriolar satellite)|go:"GO:0035253"(ciliary rootlet)|go:"GO:0035617"(stress granule disassembly)|go:"GO:0035774"(positive regulation of insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0042267"(natural killer cell mediated cytotoxicity)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0042802"(identical protein binding)|go:"GO:0043005"(neuron projection)|go:"GO:0043227"(membrane-bounded organelle)|go:"GO:0043268"(positive regulation of potassium ion transport)|go:"GO:0044295"(axonal growth cone)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0047496"(vesicle transport along microtubule)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048489"(synaptic vesicle transport)|go:"GO:0051642"(centrosome localization)|go:"GO:0071346"(cellular response to interferon-gamma)|go:"GO:0072383"(plus-end-directed vesicle transport along microtubule)|go:"GO:0090316"(positive regulation of intracellular protein transport)|go:"GO:0098971"(anterograde dendritic transport of neurotransmitter receptor complex)|go:"GO:0099609"(microtubule lateral binding)|go:"GO:0099641"(anterograde axonal protein transport)|go:"GO:1903078"(positive regulation of protein localization to plasma membrane)|go:"GO:1904115"(axon cytoplasm)|go:"GO:1905152"(positive regulation of voltage-gated sodium channel activity)|go:"GO:1990049"(retrograde neuronal dense core vesicle transport)|interpro:IPR001752(Kinesin, motor region)|interpro:IPR019821|interpro:IPR027417|interpro:IPR027640|interpro:IPR036961|mint:Q61768|rcsb pdb:6IGN|rcsb pdb:6IGV|reactome:R-MMU-2132295|reactome:R-MMU-5625970|reactome:R-MMU-6811434|reactome:R-MMU-983189 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:11aePhwWZOqqAabjyanVgdHQQso10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:Q99KJ8 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-776048|ensembl:ENSMUSP00000026479 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:dctn2_mouse(display_long)|uniprotkb:Dynactin complex 50 kDa subunit(gene name synonym)|uniprotkb:50 kDa dynein-associated polypeptide(gene name synonym)|uniprotkb:p50 dynamitin(gene name synonym)|uniprotkb:Growth cone membrane protein 23-48K(gene name synonym)|uniprotkb:Dctn2(gene name)|psi-mi:Dctn2(display_short) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_081427.1|ensembl:ENSMUSG00000025410(gene)|ensembl:ENSMUST00000026479(transcript)|go:"GO:0000278"(mitotic cell cycle)|go:"GO:0000776"(kinetochore)|go:"GO:0005737"(cytoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0005829"(cytosol)|go:"GO:0005869"(dynactin complex)|go:"GO:0005874"(microtubule)|go:"GO:0007052"(mitotic spindle organization)|go:"GO:0007080"(mitotic metaphase plate congression)|go:"GO:0016020"(membrane)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030286"(dynein complex)|go:"GO:0030426"(growth cone)|go:"GO:0030507"(spectrin binding)|go:"GO:0031982"(vesicle)|go:"GO:0032402"(melanosome transport)|go:"GO:0042802"(identical protein binding)|go:"GO:0071539"(protein localization to centrosome)|interpro:IPR028133|mint:Q99KJ8|reactome:R-MMU-2132295|reactome:R-MMU-2565942|reactome:R-MMU-3371497|reactome:R-MMU-380259|reactome:R-MMU-380270|reactome:R-MMU-380284|reactome:R-MMU-380320|reactome:R-MMU-5620912|reactome:R-MMU-6807878|reactome:R-MMU-6811436|reactome:R-MMU-8854518 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:D7u4MT5Zl5mRBQ/zsTwhYP0WHoU10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:Q9Z1T1 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-643061|uniprotkb:E9QQ08|uniprotkb:Q91YR4|ensembl:ENSMUSP00000022196 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:ap3b1_mouse(display_long)|uniprotkb:Adaptor protein complex AP-3 subunit beta-1(gene name synonym)|uniprotkb:Beta-3A-adaptin(gene name synonym)|uniprotkb:Clathrin assembly protein complex 3 beta-1 large chain(gene name synonym)|uniprotkb:Ap3b1(gene name)|psi-mi:Ap3b1(display_short)|uniprotkb:Adaptor-related protein complex 3 subunit beta-1(gene name synonym) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_033810.2|ensembl:ENSMUSG00000021686(gene)|ensembl:ENSMUST00000022196(transcript)|go:"GO:0000902"(cell morphogenesis)|go:"GO:0002224"(toll-like receptor signaling pathway)|go:"GO:0002244"(hematopoietic progenitor cell differentiation)|go:"GO:0003016"(respiratory system process)|go:"GO:0005739"(mitochondrion)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0006622"(protein targeting to lysosome)|go:"GO:0006829"(zinc ion transport)|go:"GO:0006882"(cellular zinc ion homeostasis)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006954"(inflammatory response)|go:"GO:0007040"(lysosome organization)|go:"GO:0007283"(spermatogenesis)|go:"GO:0007338"(single fertilization)|go:"GO:0007596"(blood coagulation)|go:"GO:0008089"(anterograde axonal transport)|go:"GO:0016182"(synaptic vesicle budding from endosome)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0019903"(protein phosphatase binding)|go:"GO:0030123"(AP-3 adaptor complex)|go:"GO:0030131"(clathrin adaptor complex)|go:"GO:0030324"(lung development)|go:"GO:0030665"(clathrin-coated vesicle membrane)|go:"GO:0030742"(GTP-dependent protein binding)|go:"GO:0030851"(granulocyte differentiation)|go:"GO:0032438"(melanosome organization)|go:"GO:0034394"(protein localization to cell surface)|go:"GO:0042789"(mRNA transcription by RNA polymerase II)|go:"GO:0043473"(pigmentation)|go:"GO:0045202"(synapse)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0048007"(antigen processing and presentation, exogenous lipid antigen via MHC class Ib)|go:"GO:0048490"(anterograde synaptic vesicle transport)|go:"GO:0048872"(homeostasis of number of cells)|go:"GO:0050790"(regulation of catalytic activity)|go:"GO:0051138"(positive regulation of NK T cell differentiation)|go:"GO:0060155"(platelet dense granule organization)|go:"GO:0060425"(lung morphogenesis)|go:"GO:0061024"(membrane organization)|go:"GO:0090152"(establishment of protein localization to mitochondrial membrane involved in mitochondrial fission)|go:"GO:0098773"(skin epidermis development)|go:"GO:1904115"(axon cytoplasm)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR015151(Clathrin adaptor, beta-adaptin, appendage, C-terminal subdomain)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR026739|interpro:IPR026740|interpro:IPR029390|interpro:IPR029394|mint:Q9Z1T1|reactome:R-MMU-432722|dip:DIP-49018N go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:Xtcoo5Kk0ZYpVbr+WEBfVxidkVU10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:Q8BSZ2 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-9085443|uniprotkb:O09149|uniprotkb:Q3UDG7|uniprotkb:Q8CAY1|uniprotkb:Q99589|uniprotkb:O09077|ensembl:ENSMUSP00000075082 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:ap3s2_mouse(display_long)|uniprotkb:Ap3s2(gene name)|psi-mi:Ap3s2(display_short)|uniprotkb:AP-3 complex subunit sigma-3B(gene name synonym)|uniprotkb:Sigma-3B-adaptin(gene name synonym)|uniprotkb:Sigma-adaptin 3b(gene name synonym)|uniprotkb:Adaptor-related protein complex 3 subunit sigma-2(gene name synonym) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_033812.3|ensembl:ENSMUSG00000063801(gene)|ensembl:ENSMUST00000075657(transcript)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0008089"(anterograde axonal transport)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0030123"(AP-3 adaptor complex)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0048490"(anterograde synaptic vesicle transport)|go:"GO:1904115"(axon cytoplasm)|interpro:IPR000804(Clathrin adaptor, sigma subunit/coatomer, zeta subunit)|interpro:IPR011012(Longin-like)|interpro:IPR016635(Adaptor protein complex, sigma subunit)|interpro:IPR022775|interpro:IPR027155 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:6sX3+WLoKvJc1lEegn+FyPlsbUc10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:E9Q4K7 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-9085496|ensembl:ENSMUSP00000098041 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:e9q4k7_mouse(display_long)|uniprotkb:Kif13b(gene name)|psi-mi:Kif13b(display_short) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_001074646.1|dip:DIP-61928N|ensembl:ENSMUSG00000060012(gene)|ensembl:ENSMUST00000100473(transcript)|go:"GO:0003777"(microtubule motor activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005871"(kinesin complex)|go:"GO:0005874"(microtubule)|go:"GO:0005902"(microvillus)|go:"GO:0007018"(microtubule-based movement)|go:"GO:0008017"(microtubule binding)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030424"(axon)|go:"GO:0033270"(paranode region of axon)|go:"GO:0050770"(regulation of axonogenesis)|go:"GO:0071889"(14-3-3 protein binding)|go:"GO:1990939"|interpro:IPR000253(Forkhead-associated)|interpro:IPR000938(Cytoskeleton-associated protein, CAP-Gly)|interpro:IPR001752(Kinesin, motor region)|interpro:IPR008984(SMAD/FHA domain)|interpro:IPR019821|interpro:IPR022140|interpro:IPR022164|interpro:IPR027417|interpro:IPR032405|interpro:IPR036859|interpro:IPR036961|rcsb pdb:5B64 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:FPzCTsgR0iwDPBqRiio7hJ2k6/s10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:Q9JKC8 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-9085473|uniprotkb:Q5BKQ6|ensembl:ENSMUSP00000117346 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:ap3m1_mouse(display_long)|uniprotkb:Ap3m1(gene name)|psi-mi:Ap3m1(display_short)|uniprotkb:Mu-adaptin 3A(gene name synonym)|uniprotkb:Mu3A-adaptin(gene name synonym)|uniprotkb:AP-3 adaptor complex mu3A subunit(gene name synonym)|uniprotkb:Adaptor-related protein complex 3 subunit mu-1(gene name synonym) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_061299.3|refseq:XP_006519355.1|refseq:XP_006519356.1|ensembl:ENSMUSG00000021824(gene)|ensembl:ENSMUST00000154460(transcript)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0008089"(anterograde axonal transport)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0030131"(clathrin adaptor complex)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031267"(small GTPase binding)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0048490"(anterograde synaptic vesicle transport)|go:"GO:1904115"(axon cytoplasm)|interpro:IPR001392(Clathrin adaptor, mu subunit)|interpro:IPR011012(Longin-like)|interpro:IPR018240|interpro:IPR022775|interpro:IPR028565|interpro:IPR036168|mint:Q9JKC8 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:tRkT0Uc0llSqmu/AFSjaLSqREg010090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:Q61768 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-776129|uniprotkb:Q5BL10|uniprotkb:O08711|uniprotkb:Q61580|ensembl:ENSMUSP00000025083 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:kinh_mouse(display_long)|uniprotkb:Ubiquitous kinesin heavy chain(gene name synonym)|uniprotkb:Conventional kinesin heavy chain(gene name synonym)|uniprotkb:Kif5b(gene name)|psi-mi:Kif5b(display_short)|uniprotkb:Khcs(gene name synonym)|uniprotkb:Kns1(gene name synonym) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_032474.2|dip:DIP-32053N|ensembl:ENSMUSG00000006740(gene)|ensembl:ENSMUST00000025083(transcript)|go:"GO:0003777"(microtubule motor activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005871"(kinesin complex)|go:"GO:0005874"(microtubule)|go:"GO:0006839"(mitochondrial transport)|go:"GO:0007017"(microtubule-based process)|go:"GO:0007018"(microtubule-based movement)|go:"GO:0007028"(cytoplasm organization)|go:"GO:0007411"(axon guidance)|go:"GO:0008017"(microtubule binding)|go:"GO:0008432"(JUN kinase binding)|go:"GO:0008574"(plus-end-directed microtubule motor activity)|go:"GO:0015630"(microtubule cytoskeleton)|go:"GO:0021766"(hippocampus development)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030705"(cytoskeleton-dependent intracellular transport)|go:"GO:0031340"(positive regulation of vesicle fusion)|go:"GO:0031982"(vesicle)|go:"GO:0032230"(positive regulation of synaptic transmission, GABAergic)|go:"GO:0032418"(lysosome localization)|go:"GO:0032839"(dendrite cytoplasm)|go:"GO:0034451"(centriolar satellite)|go:"GO:0035253"(ciliary rootlet)|go:"GO:0035617"(stress granule disassembly)|go:"GO:0035774"(positive regulation of insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0042267"(natural killer cell mediated cytotoxicity)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0042802"(identical protein binding)|go:"GO:0043005"(neuron projection)|go:"GO:0043227"(membrane-bounded organelle)|go:"GO:0043268"(positive regulation of potassium ion transport)|go:"GO:0044295"(axonal growth cone)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0047496"(vesicle transport along microtubule)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048489"(synaptic vesicle transport)|go:"GO:0051642"(centrosome localization)|go:"GO:0071346"(cellular response to interferon-gamma)|go:"GO:0072383"(plus-end-directed vesicle transport along microtubule)|go:"GO:0090316"(positive regulation of intracellular protein transport)|go:"GO:0098971"(anterograde dendritic transport of neurotransmitter receptor complex)|go:"GO:0099609"(microtubule lateral binding)|go:"GO:0099641"(anterograde axonal protein transport)|go:"GO:1903078"(positive regulation of protein localization to plasma membrane)|go:"GO:1904115"(axon cytoplasm)|go:"GO:1905152"(positive regulation of voltage-gated sodium channel activity)|go:"GO:1990049"(retrograde neuronal dense core vesicle transport)|interpro:IPR001752(Kinesin, motor region)|interpro:IPR019821|interpro:IPR027417|interpro:IPR027640|interpro:IPR036961|mint:Q61768|rcsb pdb:6IGN|rcsb pdb:6IGV|reactome:R-MMU-2132295|reactome:R-MMU-5625970|reactome:R-MMU-6811434|reactome:R-MMU-983189 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:11aePhwWZOqqAabjyanVgdHQQso10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:P35278 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-2551532|ensembl:ENSMUSP00000102987|uniprotkb:Q8R2N8|uniprotkb:Q8R1V8 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:rab5c_mouse(display_long)|uniprotkb:Rab5c(gene name)|psi-mi:Rab5c(display_short) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 ensembl:ENSMUSG00000019173(gene)|ensembl:ENSMUST00000107364(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0003925"(G protein activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005768"(endosome)|go:"GO:0005769"(early endosome)|go:"GO:0005811"(lipid droplet)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0007032"(endosome organization)|go:"GO:0012505"(endomembrane system)|go:"GO:0030100"(regulation of endocytosis)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0031901"(early endosome membrane)|go:"GO:0042470"(melanosome)|go:"GO:0048227"(plasma membrane to endosome transport)|go:"GO:0098993"(anchored component of synaptic vesicle membrane)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR027417|rcsb pdb:1HUQ|rcsb pdb:1Z07|rcsb pdb:1Z0D|reactome:R-MMU-432722|reactome:R-MMU-6798695|reactome:R-MMU-8856828|reactome:R-MMU-8873719|reactome:R-MMU-8876198|go:"GO:0019003"(GDP binding)|refseq:NP_077776.2|dip:DIP-56521N go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:+RBgzzjAJ3ddDtcAq0IDIzHekYE10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:Q9Z0Y1 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-2552776|ensembl:ENSMUSP00000030158|uniprotkb:Q9CR43|uniprotkb:Q9D039 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:dctn3_mouse(display_long)|uniprotkb:Dynactin light chain p24(gene name synonym)|uniprotkb:Dctn3(gene name)|psi-mi:Dctn3(display_short) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_001153037.1|refseq:NP_058586.3|ensembl:ENSMUSG00000028447(gene)|ensembl:ENSMUST00000030158(transcript)|go:"GO:0000776"(kinetochore)|go:"GO:0005730"(nucleolus)|go:"GO:0005813"(centrosome)|go:"GO:0005819"(spindle)|go:"GO:0005829"(cytosol)|go:"GO:0005869"(dynactin complex)|go:"GO:0005874"(microtubule)|go:"GO:0005875"(microtubule associated complex)|go:"GO:0007017"(microtubule-based process)|go:"GO:0030496"(midbody)|go:"GO:0032154"(cleavage furrow)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061640"(cytoskeleton-dependent cytokinesis)|interpro:IPR009991(Dynactin subunit p22)|reactome:R-MMU-2132295|reactome:R-MMU-2565942|reactome:R-MMU-3371497|reactome:R-MMU-380259|reactome:R-MMU-380270|reactome:R-MMU-380284|reactome:R-MMU-380320|reactome:R-MMU-5620912|reactome:R-MMU-6807878|reactome:R-MMU-6811436|reactome:R-MMU-8854518 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:BjcdhIIVk+9CQjJ1I4VfmjHRkAE10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:P10605 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-2924871|ensembl:ENSMUSP00000006235|uniprotkb:Q3UDW7 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:catb_mouse(display_long)|uniprotkb:Ctsb(gene name)|psi-mi:Ctsb(display_short)|uniprotkb:Cathepsin B1(gene name synonym) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 ensembl:ENSMUSG00000021939(gene)|ensembl:ENSMUST00000006235(transcript)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0005518"(collagen binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0006508"(proteolysis)|go:"GO:0006590"(thyroid hormone generation)|go:"GO:0008233"(peptidase activity)|go:"GO:0008234"(cysteine-type peptidase activity)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0030574"(collagen catabolic process)|go:"GO:0030984"(kininogen binding)|go:"GO:0042277"(peptide binding)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043394"(proteoglycan binding)|go:"GO:0043621"(protein self-association)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0046697"(decidualization)|go:"GO:0046718"(viral entry into host cell)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050790"(regulation of catalytic activity)|go:"GO:0051603"(proteolysis involved in cellular protein catabolic process)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0062023"(collagen-containing extracellular matrix)|go:"GO:0097067"(cellular response to thyroid hormone stimulus)|interpro:IPR000169(Peptidase, cysteine peptidase active site)|interpro:IPR000668(Peptidase C1A, papain C-terminal)|interpro:IPR012599(Peptidase C1A, propeptide)|interpro:IPR025660|interpro:IPR025661|go:"GO:0030855"(epithelial cell differentiation)|mint:P10605|reactome:R-MMU-1442490|reactome:R-MMU-1679131|reactome:R-MMU-2022090|reactome:R-MMU-2132295|reactome:R-MMU-6798695|interpro:IPR038765|refseq:NP_031824.1 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:iaXeLGV1ftr7OnUqbeLPZGpJ7NI10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:Q9R0M6 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-6552247|ensembl:ENSMUSP00000123325 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:rab9a_mouse(display_long)|uniprotkb:Rab9a(gene name)|psi-mi:Rab9a(display_short)|uniprotkb:Rab9(gene name synonym)|uniprotkb:Sid99(gene name synonym)|uniprotkb:Sid 99(gene name synonym) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 ensembl:ENSMUSG00000079316(gene)|ensembl:ENSMUST00000112091(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005764"(lysosome)|go:"GO:0005770"(late endosome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0015031"(protein transport)|go:"GO:0019003"(GDP binding)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0030670"(phagocytic vesicle membrane)|go:"GO:0032482"(Rab protein signal transduction)|go:"GO:0032880"(regulation of protein localization)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0042470"(melanosome)|go:"GO:0042802"(identical protein binding)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0045921"(positive regulation of exocytosis)|go:"GO:0052405"|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR027417|interpro:IPR041824|rcsb pdb:1YZL|rcsb pdb:4QXA|reactome:R-MMU-6811440|reactome:R-MMU-8873719|reactome:R-MMU-8876198|reactome:R-MMU-9706019|refseq:XP_006528985.1|refseq:NP_062747.1|dip:DIP-61068N go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:rLz70Qf4CwKMgzlwZXOhJDQK4SI10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:Q9DCR2 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-9085419|uniprotkb:O88670|ensembl:ENSMUSP00000025357 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:ap3s1_mouse(display_long)|uniprotkb:Ap3s1(gene name)|psi-mi:Ap3s1(display_short)|uniprotkb:AP-3 complex subunit sigma-3A(gene name synonym)|uniprotkb:Sigma-3A-adaptin(gene name synonym)|uniprotkb:Sigma-adaptin 3a(gene name synonym)|uniprotkb:Adaptor-related protein complex 3 subunit sigma-1(gene name synonym) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_033811.1|ensembl:ENSMUSG00000024480(gene)|ensembl:ENSMUST00000025357(transcript)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0008089"(anterograde axonal transport)|go:"GO:0016020"(membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0030123"(AP-3 adaptor complex)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:1904115"(axon cytoplasm)|interpro:IPR000804(Clathrin adaptor, sigma subunit/coatomer, zeta subunit)|interpro:IPR011012(Longin-like)|interpro:IPR016635(Adaptor protein complex, sigma subunit)|interpro:IPR022775|interpro:IPR027155|reactome:R-MMU-432722|go:"GO:0048490"(anterograde synaptic vesicle transport) go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:fRNNY4ETqt4Qv6CTQqFxeIk8Syc10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:Q9QXL2 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-911098|uniprotkb:Q6P5H1|uniprotkb:Q6ZPJ8|uniprotkb:Q8BWZ9|uniprotkb:Q8BXF1|ensembl:ENSMUSP00000085985 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:ki21a_mouse(display_long)|uniprotkb:Kif21a(gene name)|psi-mi:Kif21a(display_short)|uniprotkb:Kiaa1708(gene name synonym) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_001102510.1|refseq:NP_001102511.1|refseq:NP_001102512.1|refseq:NP_057914.2|ensembl:ENSMUSG00000022629(gene)|ensembl:ENSMUST00000088614(transcript)|go:"GO:0003777"(microtubule motor activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0005871"(kinesin complex)|go:"GO:0005874"(microtubule)|go:"GO:0005886"(plasma membrane)|go:"GO:0007018"(microtubule-based movement)|go:"GO:0008017"(microtubule binding)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0071532"(ankyrin repeat binding)|go:"GO:1990939"|interpro:IPR001680(WD40 repeat)|interpro:IPR001752(Kinesin, motor region)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR019775|interpro:IPR019821|interpro:IPR027417|interpro:IPR027640|interpro:IPR036322|interpro:IPR036961|mint:Q9QXL2|rcsb pdb:5YAY|rcsb pdb:5YBE|reactome:R-MMU-2132295|reactome:R-MMU-6811434|reactome:R-MMU-983189 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:PQNGrSKw7f5ThMhMs/glF32RiZs10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:P46460 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-398006|uniprotkb:A2A646|uniprotkb:Q8BQ65|uniprotkb:Q8C3R2|uniprotkb:Q8CCT9|uniprotkb:Q8CEF0|uniprotkb:Q923C6|ensembl:ENSMUSP00000099364 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:nsf_mouse(display_long)|uniprotkb:Nsf(gene name)|psi-mi:Nsf(display_short)|uniprotkb:Skd2(gene name synonym)|uniprotkb:N-ethylmaleimide-sensitive fusion protein(gene name synonym)|uniprotkb:Vesicular-fusion protein NSF(gene name synonym)|uniprotkb:"Suppressor of K(+) transport growth defect 2"(gene name synonym) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_032766.2|ensembl:ENSMUSG00000034187(gene)|ensembl:ENSMUST00000103075(transcript)|go:"GO:0000149"(SNARE binding)|go:"GO:0001921"(positive regulation of receptor recycling)|go:"GO:0002090"(regulation of receptor internalization)|go:"GO:0005524"(ATP binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006813"(potassium ion transport)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0014069"(postsynaptic density)|go:"GO:0015031"(protein transport)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0017075"(syntaxin-1 binding)|go:"GO:0017157"(regulation of exocytosis)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030165"(PDZ domain binding)|go:"GO:0031267"(small GTPase binding)|go:"GO:0031748"(D1 dopamine receptor binding)|go:"GO:0032984"(protein-containing complex disassembly)|go:"GO:0035255"(ionotropic glutamate receptor binding)|go:"GO:0035494"(SNARE complex disassembly)|go:"GO:0043001"(Golgi to plasma membrane protein transport)|go:"GO:0043008"(ATP-dependent protein binding)|go:"GO:0043198"(dendritic shaft)|go:"GO:0043209"(myelin sheath)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0046872"(metal ion binding)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0140545"(protein disaggregase activity)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|interpro:IPR003338(AAA ATPase VAT, N-terminal)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR003959(AAA ATPase, core)|interpro:IPR003960(AAA ATPase, conserved site)|interpro:IPR004201(Cell division protein 48, CDC48, domain 2)|interpro:IPR009010(Aspartate decarboxylase-like fold)|interpro:IPR027417|interpro:IPR029067|interpro:IPR039812|interpro:IPR041569|mint:P46460|reactome:R-MMU-204005|reactome:R-MMU-416993|reactome:R-MMU-6807878|reactome:R-MMU-6811434|reactome:R-MMU-6811438|reactome:R-MMU-6811440 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:mqNfvBVWEdD4caFZug82c88vwE410090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:O54774 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-775247|ensembl:ENSMUSP00000020420 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:ap3d1_mouse(display_long)|uniprotkb:AP-3 complex subunit delta(gene name synonym)|uniprotkb:Delta-adaptin(gene name synonym)|uniprotkb:Ap3d1(gene name)|psi-mi:Ap3d1(display_short)|uniprotkb:Ap3d(gene name synonym)|uniprotkb:Adaptor-related protein complex 3 subunit delta-1(gene name synonym) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_031486.1|dip:DIP-32163N|ensembl:ENSMUSG00000020198(gene)|ensembl:ENSMUST00000020420(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006829"(zinc ion transport)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0008089"(anterograde axonal transport)|go:"GO:0010008"(endosome membrane)|go:"GO:0016182"(synaptic vesicle budding from endosome)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0030123"(AP-3 adaptor complex)|go:"GO:0030424"(axon)|go:"GO:0033365"(protein localization to organelle)|go:"GO:0035646"(endosome to melanosome transport)|go:"GO:0043195"(terminal bouton)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0048007"(antigen processing and presentation, exogenous lipid antigen via MHC class Ib)|go:"GO:0048490"(anterograde synaptic vesicle transport)|go:"GO:0048499"(synaptic vesicle membrane organization)|go:"GO:0051138"(positive regulation of NK T cell differentiation)|go:"GO:0061088"(regulation of sequestering of zinc ion)|go:"GO:0072657"(protein localization to membrane)|go:"GO:0098793"(presynapse)|go:"GO:0098794"(postsynapse)|go:"GO:0098830"(presynaptic endosome)|go:"GO:0098943"(neurotransmitter receptor transport, postsynaptic endosome to lysosome)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099003"(vesicle-mediated transport in synapse)|go:"GO:1904115"(axon cytoplasm)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR010474(Bovine leukaemia virus receptor)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017105(Adaptor protein complex AP-3, delta subunit)|mint:O54774 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:q83u6ORB4zYNuQihE4PI1rhvSkY10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:O08788 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-776180|uniprotkb:E9QLJ1|uniprotkb:Q3TZG7|ensembl:ENSMUSP00000109552 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:dctn1_mouse(display_long)|uniprotkb:150 kDa dynein-associated polypeptide(gene name synonym)|uniprotkb:DAP-150(gene name synonym)|uniprotkb:p150-glued(gene name synonym)|uniprotkb:Dctn1(gene name)|psi-mi:Dctn1(display_short) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_031861.2|dip:DIP-32057N|ensembl:ENSMUSG00000031865(gene)|ensembl:ENSMUST00000113919(transcript)|go:"GO:0000132"(establishment of mitotic spindle orientation)|go:"GO:0000776"(kinetochore)|go:"GO:0000922"(spindle pole)|go:"GO:0005634"(nucleus)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005737"(cytoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0005814"(centriole)|go:"GO:0005819"(spindle)|go:"GO:0005829"(cytosol)|go:"GO:0005868"(cytoplasmic dynein complex)|go:"GO:0005874"(microtubule)|go:"GO:0005875"(microtubule associated complex)|go:"GO:0005938"(cell cortex)|go:"GO:0007097"(nuclear migration)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0008017"(microtubule binding)|go:"GO:0010457"(centriole-centriole cohesion)|go:"GO:0015630"(microtubule cytoskeleton)|go:"GO:0015631"(tubulin binding)|go:"GO:0019901"(protein kinase binding)|go:"GO:0021517"(ventral spinal cord development)|go:"GO:0030424"(axon)|go:"GO:0031116"(positive regulation of microtubule polymerization)|go:"GO:0031122"(cytoplasmic microtubule organization)|go:"GO:0031252"(cell leading edge)|go:"GO:0032402"(melanosome transport)|go:"GO:0032991"(protein-containing complex)|go:"GO:0034454"(microtubule anchoring at centrosome)|go:"GO:0035371"(microtubule plus-end)|go:"GO:0036064"(ciliary basal body)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0042802"(identical protein binding)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0045171"(intercellular bridge)|go:"GO:0050905"(neuromuscular process)|go:"GO:0051010"(microtubule plus-end binding)|go:"GO:0051081"(nuclear membrane disassembly)|go:"GO:0051301"(cell division)|go:"GO:0060236"(regulation of mitotic spindle organization)|go:"GO:0061744"(motor behavior)|go:"GO:0070050"(neuron cellular homeostasis)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090063"(positive regulation of microtubule nucleation)|go:"GO:0090316"(positive regulation of intracellular protein transport)|go:"GO:0099738"(cell cortex region)|go:"GO:0120103"(centriolar subdistal appendage)|go:"GO:1904398"(positive regulation of neuromuscular junction development)|go:"GO:1905515"(non-motile cilium assembly)|go:"GO:1990535"(neuron projection maintenance)|interpro:IPR000938(Cytoskeleton-associated protein, CAP-Gly)|interpro:IPR022157|interpro:IPR036859|mint:O08788|reactome:R-MMU-2132295|reactome:R-MMU-2565942|reactome:R-MMU-3371497|reactome:R-MMU-380259|reactome:R-MMU-380270|reactome:R-MMU-380284|reactome:R-MMU-380320|reactome:R-MMU-5620912|reactome:R-MMU-6807878|reactome:R-MMU-6811436|reactome:R-MMU-8854518 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:pqj7TtNDgexEbxqLwksW3eW41bo10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:Q9QZB9 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-6690332|ensembl:ENSMUSP00000033156|uniprotkb:Q3U9A4|uniprotkb:Q8BPE5|uniprotkb:Q91XC6|uniprotkb:Q99J17 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:dctn5_mouse(display_long)|uniprotkb:Dctn5(gene name)|psi-mi:Dctn5(display_short)|uniprotkb:Dynactin subunit p25(gene name synonym) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 ensembl:ENSMUST00000033156(transcript)|go:"GO:0000776"(kinetochore)|go:"GO:0003281"(ventricular septum development)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0031965"(nuclear membrane)|go:"GO:0035904"(aorta development)|go:"GO:0060976"(coronary vasculature development)|interpro:IPR001451(Bacterial transferase hexapeptide repeat)|interpro:IPR011004(Trimeric LpxA-like)|reactome:R-MMU-2132295|reactome:R-MMU-3371497|reactome:R-MMU-6807878|reactome:R-MMU-6811436|ensembl:ENSMUSG00000030868(gene)|refseq:NP_067621.3 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:caGCjSAo1hoPsWn90W0msQDRKjs10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:P46638 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-1634944|ensembl:ENSMUSP00000110021 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:rb11b_mouse(display_long)|uniprotkb:Rab11b(gene name)|psi-mi:Rab11b(display_short) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_033023.1|ensembl:ENSMUSG00000077450(gene)|ensembl:ENSMUST00000057373(transcript)|go:"GO:0001881"(receptor recycling)|go:"GO:0003924"(GTPase activity)|go:"GO:0003925"(G protein activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0006887"(exocytosis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0019003"(GDP binding)|go:"GO:0030670"(phagocytic vesicle membrane)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031489"(myosin V binding)|go:"GO:0032402"(melanosome transport)|go:"GO:0032456"(endocytic recycling)|go:"GO:0033572"(transferrin transport)|go:"GO:0035773"(insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0044070"(regulation of anion transport)|go:"GO:0045054"(constitutive secretory pathway)|go:"GO:0045055"(regulated exocytosis)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0055037"(recycling endosome)|go:"GO:0055038"(recycling endosome membrane)|go:"GO:0071468"(cellular response to acidic pH)|go:"GO:0090150"(establishment of protein localization to membrane)|go:"GO:0098993"(anchored component of synaptic vesicle membrane)|go:"GO:0150093"(amyloid-beta clearance by transcytosis)|go:"GO:2000008"(regulation of protein localization to cell surface)|go:"GO:2001135"(regulation of endocytic recycling)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR027417|mint:P46638|reactome:R-MMU-8854214|reactome:R-MMU-8873719 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:McbcmcV5cB7IE3GFkiDNIB5WcsM10090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q8BKC8 uniprotkb:Q9JHU4 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 intact:EBI-645061|uniprotkb:E9QM71|ensembl:ENSMUSP00000018851 psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:dyhc1_mouse(display_long)|uniprotkb:Dhc1(gene name synonym)|uniprotkb:Dyhc(gene name synonym)|uniprotkb:Cytoplasmic dynein heavy chain 1(gene name synonym)|uniprotkb:Dynein heavy chain, cytosolic(gene name synonym)|uniprotkb:Dync1h1(gene name)|psi-mi:Dync1h1(display_short)|uniprotkb:Dnch1(gene name synonym)|uniprotkb:Dnchc1(gene name synonym) psi-mi:"MI:0096"(pull down) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0914"(association) psi-mi:"MI:0471"(MINT) intact:EBI-9085471|imex:IM-22093-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 refseq:NP_084514.2|ensembl:ENSMUSG00000018707(gene)|go:"GO:0000278"(mitotic cell cycle)|go:"GO:0002177"(manchette)|go:"GO:0003341"(cilium movement)|go:"GO:0005524"(ATP binding)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005737"(cytoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0005868"(cytoplasmic dynein complex)|go:"GO:0005874"(microtubule)|go:"GO:0005881"(cytoplasmic microtubule)|go:"GO:0005938"(cell cortex)|go:"GO:0007018"(microtubule-based movement)|go:"GO:0007097"(nuclear migration)|go:"GO:0008090"(retrograde axonal transport)|go:"GO:0008569"(minus-end-directed microtubule motor activity)|go:"GO:0030175"(filopodium)|go:"GO:0030286"(dynein complex)|go:"GO:0030424"(axon)|go:"GO:0031122"(cytoplasmic microtubule organization)|go:"GO:0032388"(positive regulation of intracellular transport)|go:"GO:0033962"(P-body assembly)|go:"GO:0034063"(stress granule assembly)|go:"GO:0043025"(neuronal cell body)|go:"GO:0045505"(dynein intermediate chain binding)|go:"GO:0051293"(establishment of spindle localization)|go:"GO:0051301"(cell division)|go:"GO:0051959"(dynein light intermediate chain binding)|go:"GO:0060236"(regulation of mitotic spindle organization)|go:"GO:0072382"(minus-end-directed vesicle transport along microtubule)|go:"GO:0090235"(regulation of metaphase plate congression)|go:"GO:0120162"(positive regulation of cold-induced thermogenesis)|go:"GO:1904115"(axon cytoplasm)|go:"GO:1905832"(positive regulation of spindle assembly)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR004273(Dynein heavy chain)|interpro:IPR013594(Dynein heavy chain, N-terminal region 1)|interpro:IPR013602(Dynein heavy chain, N-terminal region 2)|interpro:IPR024317|interpro:IPR024743|interpro:IPR027417|interpro:IPR035699|interpro:IPR035706|interpro:IPR041228|interpro:IPR041466|interpro:IPR041658|ensembl:ENSMUST00000018851(transcript)|interpro:IPR042222|interpro:IPR042228|interpro:IPR043157|interpro:IPR043160|interpro:IPR042219|mint:Q9JHU4|rcsb pdb:3ERR|rcsb pdb:3J1T|rcsb pdb:3J1U|rcsb pdb:3WUQ|rcsb pdb:5AYH|rcsb pdb:6RZA|rcsb pdb:6RZB|reactome:R-MMU-141444|reactome:R-MMU-2132295|reactome:R-MMU-2467813|reactome:R-MMU-2500257|reactome:R-MMU-2565942|reactome:R-MMU-3371497|reactome:R-MMU-380259|reactome:R-MMU-380270|reactome:R-MMU-380284|reactome:R-MMU-380320|reactome:R-MMU-5620912|reactome:R-MMU-5663220|reactome:R-MMU-6798695|reactome:R-MMU-6807878|reactome:R-MMU-6811436|reactome:R-MMU-68877|reactome:R-MMU-8854518|reactome:R-MMU-9646399|reactome:R-MMU-9648025 go:"GO:0033107"(Cvt vesicle) - - figure legend:sf7b|comment:"Proteomic analysis of the vesicles positive for PI4KIIIb, purified out of the smaller vesicular fraction using anti- PI4KIIIb-conjugated magnetic beads, revealed that the most abundant proteins in these vesicular carriers fall into one of these three categories: clathrin and adaptor molecules, proteins involved in vesicular fusion/fission and motor proteins (Supplementary Figure S7B)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-3t3)|taxid:10090(Mus musculus embryo cells) - 2014/01/15 2014/10/16 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 rogid:sbED0xqQpe32T7I8ijeS3Xqlx7010090 intact-crc:3B08C7CEF27D02F5|rigid:aVCMiIwotgkXR+rfjvaL2f7cjm4 false - - - - psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:P17047 uniprotkb:Q9UBF8 intact:EBI-1564749|uniprotkb:A2A430|uniprotkb:Q3TXG5|uniprotkb:Q8BSG8|ensembl:ENSMUSP00000016678 intact:EBI-1053214|uniprotkb:B4DGI2|uniprotkb:O15096|uniprotkb:P78405|uniprotkb:Q5VWC1|uniprotkb:Q9BWR6|uniprotkb:Q5VWB9|uniprotkb:Q5VWC0|ensembl:ENSP00000357867 psi-mi:lamp2_mouse(display_long)|uniprotkb:Lysosomal membrane glycoprotein type B(gene name synonym)|uniprotkb:CD107 antigen-like family member B(gene name synonym)|uniprotkb:Lamp2(gene name)|psi-mi:Lamp2(display_short)|uniprotkb:Lamp-2(gene name synonym) psi-mi:pi4kb_human(display_long)|uniprotkb:NPIK(gene name synonym)|uniprotkb:PI4K92(gene name synonym)|uniprotkb:PI4KIII(gene name synonym)|uniprotkb:PI4KB(gene name)|psi-mi:PI4KB(display_short)|uniprotkb:PIK4CB(gene name synonym) psi-mi:"MI:0416"(fluorescence microscopy) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103729|imex:IM-22093-8 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001017959.1|refseq:NP_034815.2|ensembl:ENSMUSG00000016534(gene)|ensembl:ENSMUST00000016678(transcript)|go:"GO:0005615"(extracellular space)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005768"(endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0006605"(protein targeting)|go:"GO:0006914"(autophagy)|go:"GO:0009267"(cellular response to starvation)|go:"GO:0016020"(membrane)|go:"GO:0019899"(enzyme binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030670"(phagocytic vesicle membrane)|go:"GO:0031088"(platelet dense granule membrane)|go:"GO:0031333"(negative regulation of protein-containing complex assembly)|go:"GO:0031647"(regulation of protein stability)|go:"GO:0031902"(late endosome membrane)|go:"GO:0044754"(autolysosome)|go:"GO:0046716"(muscle cell cellular homeostasis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050821"(protein stabilization)|go:"GO:0061684"(chaperone-mediated autophagy)|go:"GO:0061740"(protein targeting to lysosome involved in chaperone-mediated autophagy)|go:"GO:0061742"(chaperone-mediated autophagy translocation complex)|go:"GO:0070062"(extracellular exosome)|go:"GO:0072594"(establishment of protein localization to organelle)|go:"GO:0097352"(autophagosome maturation)|go:"GO:0097637"(integral component of autophagosome membrane)|go:"GO:1905146"(lysosomal protein catabolic process)|go:"GO:1990836"(lysosomal matrix)|interpro:IPR002000("Lysosome-associated membrane glycoprotein (Lamp)/CD68")|interpro:IPR018134|mint:P17047|rcsb pdb:5GV3|reactome:R-MMU-114608|reactome:R-MMU-6798695 refseq:NP_001185702.1|refseq:NP_001185703.1|refseq:NP_001185704.1|refseq:NP_002642.1|ensembl:ENSG00000143393(gene)|ensembl:ENST00000368873(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005768"(endosome)|go:"GO:0005829"(cytosol)|go:"GO:0006661"(phosphatidylinositol biosynthetic process)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0007165"(signal transduction)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|mint:Q9UBF8|rcsb pdb:2N73|rcsb pdb:4D0L|rcsb pdb:4D0M|rcsb pdb:4WAE|rcsb pdb:4WAG|rcsb pdb:5C46|rcsb pdb:5C4G|rcsb pdb:5EUQ|rcsb pdb:5FBL|rcsb pdb:5FBQ|rcsb pdb:5FBR|rcsb pdb:5FBV|rcsb pdb:5FBW|rcsb pdb:5LX2|rcsb pdb:5NAS|rcsb pdb:6GL3|reactome:R-HSA-1660514|refseq:NP_001317650.1|refseq:XP_016856979.1|refseq:XP_016856980.1|refseq:XP_016856981.1|dip:DIP-42267N go:"GO:0005764"(lysosome) - - figure legend:f5b|comment:"Immunofluorescence for PI4KIIIβ in cultured cells (Figure 5B) confirmed its colocalization with LAMP1 (18.7±5%) and LAMP2 (26.9±1.3%) in agreement with the levels found when Lys were isolated from these cells (Supplementary Figure S6A)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9534(chlae-cos_7)|taxid:9534(Cercopithecus aethiops simian cells transformed with SV40) - 2014/01/15 2014/10/16 rogid:em1v9qxpoHR13ocQdAffj+zQSDE10090 rogid:EeU4q6eJ1Kji9EaWCT+xYv2Y8eA9606 intact-crc:686DC78A8E846431|rigid:qcIWbvf7lqLs1VY3LwGdBbk3X7M false - - - - psi-mi:"MI:0422"(immunostaining) psi-mi:"MI:0422"(immunostaining) uniprotkb:P11438 uniprotkb:Q9UBF8 intact:EBI-987439|uniprotkb:Q62020|ensembl:ENSMUSP00000033824 intact:EBI-1053214|uniprotkb:B4DGI2|uniprotkb:O15096|uniprotkb:P78405|uniprotkb:Q5VWC1|uniprotkb:Q9BWR6|uniprotkb:Q5VWB9|uniprotkb:Q5VWC0|ensembl:ENSP00000357867 psi-mi:lamp1_mouse(display_long)|uniprotkb:Lysosomal membrane glycoprotein A(gene name synonym)|uniprotkb:120 kDa lysosomal membrane glycoprotein(gene name synonym)|uniprotkb:LGP-120(gene name synonym)|uniprotkb:P2B(gene name synonym)|uniprotkb:CD107 antigen-like family member A(gene name synonym)|uniprotkb:Lamp1(gene name)|psi-mi:Lamp1(display_short)|uniprotkb:Lamp-1(gene name synonym) psi-mi:pi4kb_human(display_long)|uniprotkb:NPIK(gene name synonym)|uniprotkb:PI4K92(gene name synonym)|uniprotkb:PI4KIII(gene name synonym)|uniprotkb:PI4KB(gene name)|psi-mi:PI4KB(display_short)|uniprotkb:PIK4CB(gene name synonym) psi-mi:"MI:0416"(fluorescence microscopy) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103723|imex:IM-22093-7 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSMUSG00000031447(gene)|ensembl:ENSMUST00000033824(transcript)|go:"GO:0000421"(autophagosome membrane)|go:"GO:0005737"(cytoplasm)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005768"(endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005771"(multivesicular body)|go:"GO:0005773"(vacuole)|refseq:NP_034814.2|go:"GO:0005886"(plasma membrane)|go:"GO:0007283"(spermatogenesis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0010008"(endosome membrane)|go:"GO:0019899"(enzyme binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030425"(dendrite)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031902"(late endosome membrane)|go:"GO:0031982"(vesicle)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0044194"(cytolytic granule)|go:"GO:0044754"(autolysosome)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048102"(autophagic cell death)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050821"(protein stabilization)|go:"GO:0061474"(phagolysosome membrane)|go:"GO:0072594"(establishment of protein localization to organelle)|go:"GO:0005829"(cytosol)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0097208"(alveolar lamellar body)|go:"GO:0101004"(cytolytic granule membrane)|go:"GO:0140507"(granzyme-mediated programmed cell death signaling pathway)|go:"GO:1902513"(regulation of organelle transport along microtubule)|interpro:IPR002000("Lysosome-associated membrane glycoprotein (Lamp)/CD68")|interpro:IPR018134|mint:P11438|rcsb pdb:5GV0|reactome:R-MMU-6798695|refseq:NP_001304282.1 refseq:NP_001185702.1|refseq:NP_001185703.1|refseq:NP_001185704.1|refseq:NP_002642.1|ensembl:ENSG00000143393(gene)|ensembl:ENST00000368873(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005768"(endosome)|go:"GO:0005829"(cytosol)|go:"GO:0006661"(phosphatidylinositol biosynthetic process)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0007165"(signal transduction)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|mint:Q9UBF8|rcsb pdb:2N73|rcsb pdb:4D0L|rcsb pdb:4D0M|rcsb pdb:4WAE|rcsb pdb:4WAG|rcsb pdb:5C46|rcsb pdb:5C4G|rcsb pdb:5EUQ|rcsb pdb:5FBL|rcsb pdb:5FBQ|rcsb pdb:5FBR|rcsb pdb:5FBV|rcsb pdb:5FBW|rcsb pdb:5LX2|rcsb pdb:5NAS|rcsb pdb:6GL3|reactome:R-HSA-1660514|refseq:NP_001317650.1|refseq:XP_016856979.1|refseq:XP_016856980.1|refseq:XP_016856981.1|dip:DIP-42267N go:"GO:0005764"(lysosome) - - figure legend:f5b|comment:"Immunofluorescence for PI4KIIIβ in cultured cells (Figure 5B) confirmed its colocalization with LAMP1 (18.7±5%) and LAMP2 (26.9±1.3%) in agreement with the levels found when Lys were isolated from these cells (Supplementary Figure S6A)."|comment:"Pi4kb has been detected as endougenously expressed in green monkey cells (COS-7); since its accession number needs to be updated we have adopted the human isoform of the protein, because the antibody also recognizes the human isoform. Lamp-1 has been detected as endougenously expressed in green monkey cells (COS-7); since its accession number needs to be updated we have adopted the mouse isoform of the protein, because the antibody also recognizes the mouse isoform."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9534(chlae-cos_7)|taxid:9534(Cercopithecus aethiops simian cells transformed with SV40) - 2014/01/15 2014/10/16 rogid:34kBKJCpWncY9gLqL2lEx3Yn01I10090 rogid:EeU4q6eJ1Kji9EaWCT+xYv2Y8eA9606 intact-crc:579BCF32E6F351EE|rigid:PCze/b63l2fuJ0vDRyDumaeEmlI false - - - - psi-mi:"MI:0422"(immunostaining) psi-mi:"MI:0422"(immunostaining) uniprotkb:P11438 uniprotkb:P17047-1 intact:EBI-987439|uniprotkb:Q62020|ensembl:ENSMUSP00000033824 intact:EBI-9103451|ensembl:ENSMUSP00000016678 psi-mi:lamp1_mouse(display_long)|uniprotkb:Lysosomal membrane glycoprotein A(gene name synonym)|uniprotkb:120 kDa lysosomal membrane glycoprotein(gene name synonym)|uniprotkb:LGP-120(gene name synonym)|uniprotkb:P2B(gene name synonym)|uniprotkb:CD107 antigen-like family member A(gene name synonym)|uniprotkb:Lamp1(gene name)|psi-mi:Lamp1(display_short)|uniprotkb:Lamp-1(gene name synonym) psi-mi:p17047-1(display_long)|uniprotkb:Lamp2(gene name)|psi-mi:Lamp2(display_short)|uniprotkb:Lamp-2(gene name synonym)|uniprotkb:CD107 antigen-like family member B(gene name synonym)|uniprotkb:Lysosomal membrane glycoprotein type B(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103547|imex:IM-22093-6 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSMUSG00000031447(gene)|ensembl:ENSMUST00000033824(transcript)|go:"GO:0000421"(autophagosome membrane)|go:"GO:0005737"(cytoplasm)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005768"(endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005771"(multivesicular body)|go:"GO:0005773"(vacuole)|refseq:NP_034814.2|go:"GO:0005886"(plasma membrane)|go:"GO:0007283"(spermatogenesis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0010008"(endosome membrane)|go:"GO:0019899"(enzyme binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030425"(dendrite)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031902"(late endosome membrane)|go:"GO:0031982"(vesicle)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0044194"(cytolytic granule)|go:"GO:0044754"(autolysosome)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048102"(autophagic cell death)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050821"(protein stabilization)|go:"GO:0061474"(phagolysosome membrane)|go:"GO:0072594"(establishment of protein localization to organelle)|go:"GO:0005829"(cytosol)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0097208"(alveolar lamellar body)|go:"GO:0101004"(cytolytic granule membrane)|go:"GO:0140507"(granzyme-mediated programmed cell death signaling pathway)|go:"GO:1902513"(regulation of organelle transport along microtubule)|interpro:IPR002000("Lysosome-associated membrane glycoprotein (Lamp)/CD68")|interpro:IPR018134|mint:P11438|rcsb pdb:5GV0|reactome:R-MMU-6798695|refseq:NP_001304282.1 intact:EBI-1564749(isoform-parent)|ensembl:ENSMUSG00000016534(gene)|ensembl:ENSMUST00000016678(transcript) go:"GO:0005765"(lysosomal membrane) - - figure legend:f5c|comment:"We next purified Lys from control and PI4KIIIβ(−) using a previously standardized protocol that allows for the isolation of a fraction highly enriched in secondary Lys (the use of floatation gradients made lysosomal isolation comparable in both groups independent of the changes in sedimentation behaviour observed in Supplementary Figure S5C (see also Supplementary data; Aniento et al, 1993; Cuervo et al, 1997; Bandyopadhyay et al, 2010; Koga et al, 2010). Immunoblot revealed a significant reduction in the levels of integral membrane proteins (LAMP1, LAMP‐2A, LAMP‐2B, V‐ATPase) and resident lumenal enzymes (Cathepsin B, Cathepsin D, Cathepsin L) (Figure 4C)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:34kBKJCpWncY9gLqL2lEx3Yn01I10090 rogid:em1v9qxpoHR13ocQdAffj+zQSDE10090 intact-crc:DF7822EE7A66B302|rigid:VXEOYFe4ja7TWugC/11zKf2I1xI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P11438 uniprotkb:Q91YH6 intact:EBI-987439|uniprotkb:Q62020|ensembl:ENSMUSP00000033824 intact:EBI-8365420|uniprotkb:Q6P6I3|uniprotkb:Q8C3L6|intact:MINT-4121894|ensembl:ENSMUSP00000006431 psi-mi:lamp1_mouse(display_long)|uniprotkb:Lysosomal membrane glycoprotein A(gene name synonym)|uniprotkb:120 kDa lysosomal membrane glycoprotein(gene name synonym)|uniprotkb:LGP-120(gene name synonym)|uniprotkb:P2B(gene name synonym)|uniprotkb:CD107 antigen-like family member A(gene name synonym)|uniprotkb:Lamp1(gene name)|psi-mi:Lamp1(display_short)|uniprotkb:Lamp-1(gene name synonym) psi-mi:vatb1_mouse(display_long)|uniprotkb:Atp6v1b1(gene name)|psi-mi:Atp6v1b1(display_short)|uniprotkb:Atp6b1(gene name synonym)|uniprotkb:Endomembrane proton pump 58 kDa subunit(gene name synonym)|uniprotkb:Vacuolar proton pump subunit B 1(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103547|imex:IM-22093-6 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSMUSG00000031447(gene)|ensembl:ENSMUST00000033824(transcript)|go:"GO:0000421"(autophagosome membrane)|go:"GO:0005737"(cytoplasm)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005768"(endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005771"(multivesicular body)|go:"GO:0005773"(vacuole)|refseq:NP_034814.2|go:"GO:0005886"(plasma membrane)|go:"GO:0007283"(spermatogenesis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0010008"(endosome membrane)|go:"GO:0019899"(enzyme binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030425"(dendrite)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031902"(late endosome membrane)|go:"GO:0031982"(vesicle)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0044194"(cytolytic granule)|go:"GO:0044754"(autolysosome)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048102"(autophagic cell death)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050821"(protein stabilization)|go:"GO:0061474"(phagolysosome membrane)|go:"GO:0072594"(establishment of protein localization to organelle)|go:"GO:0005829"(cytosol)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0097208"(alveolar lamellar body)|go:"GO:0101004"(cytolytic granule membrane)|go:"GO:0140507"(granzyme-mediated programmed cell death signaling pathway)|go:"GO:1902513"(regulation of organelle transport along microtubule)|interpro:IPR002000("Lysosome-associated membrane glycoprotein (Lamp)/CD68")|interpro:IPR018134|mint:P11438|rcsb pdb:5GV0|reactome:R-MMU-6798695|refseq:NP_001304282.1 refseq:NP_598918.1|ensembl:ENSMUSG00000006269(gene)|ensembl:ENSMUST00000006431(transcript)|go:"GO:0000221"(vacuolar proton-transporting V-type ATPase, V1 domain)|go:"GO:0001503"(ossification)|go:"GO:0003091"(renal water homeostasis)|go:"GO:0003096"(renal sodium ion transport)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005902"(microvillus)|go:"GO:0006693"(prostaglandin metabolic process)|go:"GO:0006885"(regulation of pH)|go:"GO:0007588"(excretion)|go:"GO:0007605"(sensory perception of sound)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0010468"(regulation of gene expression)|go:"GO:0015078"(proton transmembrane transporter activity)|go:"GO:0016020"(membrane)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0016471"(vacuolar proton-transporting V-type ATPase complex)|go:"GO:0016787"(hydrolase activity)|go:"GO:0030534"(adult behavior)|go:"GO:0035812"(renal sodium excretion)|go:"GO:0042048"(olfactory behavior)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045851"(pH reduction)|go:"GO:0046034"(ATP metabolic process)|go:"GO:0055064"(chloride ion homeostasis)|go:"GO:0055074"(calcium ion homeostasis)|go:"GO:0055075"(potassium ion homeostasis)|go:"GO:0070072"(vacuolar proton-transporting V-type ATPase complex assembly)|go:"GO:0098850"(extrinsic component of synaptic vesicle membrane)|go:"GO:1902600"(proton transmembrane transport)|interpro:IPR000194(ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding)|interpro:IPR004100(ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal)|interpro:IPR005723(ATPase, V1 complex, subunit B)|interpro:IPR020003|interpro:IPR022879|interpro:IPR027417|mint:Q91YH6|reactome:R-MMU-1222556|reactome:R-MMU-77387|reactome:R-MMU-917977|reactome:R-MMU-9639288|reactome:R-MMU-983712 go:"GO:0005765"(lysosomal membrane) - comment:mint figure legend:f5c|comment:"We next purified Lys from control and PI4KIIIβ(−) using a previously standardized protocol that allows for the isolation of a fraction highly enriched in secondary Lys (the use of floatation gradients made lysosomal isolation comparable in both groups independent of the changes in sedimentation behaviour observed in Supplementary Figure S5C (see also Supplementary data; Aniento et al, 1993; Cuervo et al, 1997; Bandyopadhyay et al, 2010; Koga et al, 2010). Immunoblot revealed a significant reduction in the levels of integral membrane proteins (LAMP1, LAMP‐2A, LAMP‐2B, V‐ATPase) and resident lumenal enzymes (Cathepsin B, Cathepsin D, Cathepsin L) (Figure 4C)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:34kBKJCpWncY9gLqL2lEx3Yn01I10090 rogid:BN28VewxPJE0pRYUsD6ExW5+qzI10090 intact-crc:DF7822EE7A66B302|rigid:VXEOYFe4ja7TWugC/11zKf2I1xI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P10605 uniprotkb:P18242 intact:EBI-2924871|ensembl:ENSMUSP00000006235|uniprotkb:Q3UDW7 intact:EBI-738562|ensembl:ENSMUSP00000121203 psi-mi:catb_mouse(display_long)|uniprotkb:Ctsb(gene name)|psi-mi:Ctsb(display_short)|uniprotkb:Cathepsin B1(gene name synonym) psi-mi:catd_mouse(display_long)|uniprotkb:Ctsd(gene name)|psi-mi:Ctsd(display_short) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103449|imex:IM-22093-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSMUSG00000021939(gene)|ensembl:ENSMUST00000006235(transcript)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0005518"(collagen binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0006508"(proteolysis)|go:"GO:0006590"(thyroid hormone generation)|go:"GO:0008233"(peptidase activity)|go:"GO:0008234"(cysteine-type peptidase activity)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0030574"(collagen catabolic process)|go:"GO:0030984"(kininogen binding)|go:"GO:0042277"(peptide binding)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043394"(proteoglycan binding)|go:"GO:0043621"(protein self-association)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0046697"(decidualization)|go:"GO:0046718"(viral entry into host cell)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050790"(regulation of catalytic activity)|go:"GO:0051603"(proteolysis involved in cellular protein catabolic process)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0062023"(collagen-containing extracellular matrix)|go:"GO:0097067"(cellular response to thyroid hormone stimulus)|interpro:IPR000169(Peptidase, cysteine peptidase active site)|interpro:IPR000668(Peptidase C1A, papain C-terminal)|interpro:IPR012599(Peptidase C1A, propeptide)|interpro:IPR025660|interpro:IPR025661|go:"GO:0030855"(epithelial cell differentiation)|mint:P10605|reactome:R-MMU-1442490|reactome:R-MMU-1679131|reactome:R-MMU-2022090|reactome:R-MMU-2132295|reactome:R-MMU-6798695|interpro:IPR038765|refseq:NP_031824.1 refseq:NP_034113.1|go:"GO:0042159"(lipoprotein catabolic process)|go:"GO:0042277"(peptide binding)|go:"GO:0042470"(melanosome)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0045121"(membrane raft)|go:"GO:0062023"(collagen-containing extracellular matrix)|go:"GO:0070001"(aspartic-type peptidase activity)|go:"GO:0070201"(regulation of establishment of protein localization)|interpro:IPR001461(Peptidase A1)|interpro:IPR001969(Peptidase aspartic, active site)|interpro:IPR012848(Propeptide, peptidase A1)|go:"GO:0004190"(aspartic-type endopeptidase activity)|interpro:IPR021109|interpro:IPR033121|interpro:IPR033144|mint:P18242|go:"GO:0005615"(extracellular space)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|reactome:R-MMU-1442490|go:"GO:0006508"(proteolysis)|go:"GO:0008233"(peptidase activity)|reactome:R-MMU-2022377|reactome:R-MMU-2132295|reactome:R-MMU-6798695|go:"GO:0010008"(endosome membrane)|go:"GO:0016787"(hydrolase activity)|ensembl:ENSMUSG00000007891(gene)|ensembl:ENSMUST00000151120(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0004175"(endopeptidase activity) go:"GO:0005764"(lysosome) - - figure legend:f4c f5c|comment:"We next purified Lys from control and PI4KIIIβ(−) using a previously standardized protocol that allows for the isolation of a fraction highly enriched in secondary Lys (the use of floatation gradients made lysosomal isolation comparable in both groups independent of the changes in sedimentation behaviour observed in Supplementary Figure S5C (see also Supplementary data; Aniento et al, 1993; Cuervo et al, 1997; Bandyopadhyay et al, 2010; Koga et al, 2010). Immunoblot revealed a significant reduction in the levels of integral membrane proteins (LAMP1, LAMP‐2A, LAMP‐2B, V‐ATPase) and resident lumenal enzymes (Cathepsin B, Cathepsin D, Cathepsin L) (Figure 4C)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:iaXeLGV1ftr7OnUqbeLPZGpJ7NI10090 rogid:PrpcvWCAFSmTF9ECBCB/zMV6d9M10090 intact-crc:E7A58C754C6C2571|rigid:mjP94ovRpppRodGMuDvzGR5l+r8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P10605 uniprotkb:P06797 intact:EBI-2924871|ensembl:ENSMUSP00000006235|uniprotkb:Q3UDW7 intact:EBI-7876993|uniprotkb:Q91UZ0|intact:MINT-1863740|ensembl:ENSMUSP00000021933|ensembl:ENSMUSP00000152169 psi-mi:catb_mouse(display_long)|uniprotkb:Ctsb(gene name)|psi-mi:Ctsb(display_short)|uniprotkb:Cathepsin B1(gene name synonym) psi-mi:catl1_mouse(display_long)|uniprotkb:Ctsl(gene name)|psi-mi:Ctsl(display_short)|uniprotkb:Ctsl1(gene name synonym)|uniprotkb:Major excreted protein(gene name synonym)|uniprotkb:p39 cysteine proteinase(gene name synonym)|uniprotkb:Cathepsin L1(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103449|imex:IM-22093-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSMUSG00000021939(gene)|ensembl:ENSMUST00000006235(transcript)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0005518"(collagen binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0006508"(proteolysis)|go:"GO:0006590"(thyroid hormone generation)|go:"GO:0008233"(peptidase activity)|go:"GO:0008234"(cysteine-type peptidase activity)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0030574"(collagen catabolic process)|go:"GO:0030984"(kininogen binding)|go:"GO:0042277"(peptide binding)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043394"(proteoglycan binding)|go:"GO:0043621"(protein self-association)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0046697"(decidualization)|go:"GO:0046718"(viral entry into host cell)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050790"(regulation of catalytic activity)|go:"GO:0051603"(proteolysis involved in cellular protein catabolic process)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0062023"(collagen-containing extracellular matrix)|go:"GO:0097067"(cellular response to thyroid hormone stimulus)|interpro:IPR000169(Peptidase, cysteine peptidase active site)|interpro:IPR000668(Peptidase C1A, papain C-terminal)|interpro:IPR012599(Peptidase C1A, propeptide)|interpro:IPR025660|interpro:IPR025661|go:"GO:0030855"(epithelial cell differentiation)|mint:P10605|reactome:R-MMU-1442490|reactome:R-MMU-1679131|reactome:R-MMU-2022090|reactome:R-MMU-2132295|reactome:R-MMU-6798695|interpro:IPR038765|refseq:NP_031824.1 refseq:NP_034114.1|refseq:XP_006517143.1|go:"GO:0043373"(CD4-positive, alpha-beta T cell lineage commitment)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0046697"(decidualization)|go:"GO:0048002"(antigen processing and presentation of peptide antigen)|go:"GO:0048102"(autophagic cell death)|go:"GO:0051384"(response to glucocorticoid)|go:"GO:0051603"(proteolysis involved in cellular protein catabolic process)|go:"GO:0060008"(Sertoli cell differentiation)|go:"GO:0060309"(elastin catabolic process)|go:"GO:0062023"(collagen-containing extracellular matrix)|interpro:IPR000169(Peptidase, cysteine peptidase active site)|interpro:IPR000668(Peptidase C1A, papain C-terminal)|interpro:IPR013201(Proteinase inhibitor I29, cathepsin propeptide)|go:"GO:0043204"(perikaryon)|interpro:IPR025660|interpro:IPR025661|interpro:IPR038765|interpro:IPR039417|rcsb pdb:1MVV|reactome:R-MMU-1442490|reactome:R-MMU-1474228|reactome:R-MMU-1679131|reactome:R-MMU-2022090|reactome:R-MMU-2132295|reactome:R-MMU-8939242|ensembl:ENSMUSG00000021477(gene)|ensembl:ENSMUST00000021933(transcript)|ensembl:ENSMUST00000222517(transcript)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004177"(aminopeptidase activity)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005634"(nucleus)|go:"GO:0005730"(nucleolus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005764"(lysosome)|go:"GO:0005773"(vacuole)|go:"GO:0005902"(microvillus)|go:"GO:0006508"(proteolysis)|go:"GO:0006590"(thyroid hormone generation)|go:"GO:0006955"(immune response)|go:"GO:0007154"(cell communication)|go:"GO:0007283"(spermatogenesis)|go:"GO:0008584"(male gonad development)|go:"GO:0009749"(response to glucose)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0010259"(multicellular organism aging)|go:"GO:0010839"(negative regulation of keratinocyte proliferation)|go:"GO:0014070"(response to organic cyclic compound)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0016807"(cysteine-type carboxypeptidase activity)|go:"GO:0021675"(nerve development)|go:"GO:0030574"(collagen catabolic process)|go:"GO:0030984"(kininogen binding)|go:"GO:0031069"(hair follicle morphogenesis)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031638"(zymogen activation)|go:"GO:0034230"(enkephalin processing)|go:"GO:0034698"(response to gonadotropin)|go:"GO:0042277"(peptide binding)|go:"GO:0042393"(histone binding)|go:"GO:0042583"(chromaffin granule)|go:"GO:0009267"(cellular response to starvation)|go:"GO:0043005"(neuron projection) go:"GO:0005764"(lysosome) - comment:mint|function:Important for the overall degradation of proteins in lysosomes figure legend:f4c f5c|comment:"We next purified Lys from control and PI4KIIIβ(−) using a previously standardized protocol that allows for the isolation of a fraction highly enriched in secondary Lys (the use of floatation gradients made lysosomal isolation comparable in both groups independent of the changes in sedimentation behaviour observed in Supplementary Figure S5C (see also Supplementary data; Aniento et al, 1993; Cuervo et al, 1997; Bandyopadhyay et al, 2010; Koga et al, 2010). Immunoblot revealed a significant reduction in the levels of integral membrane proteins (LAMP1, LAMP‐2A, LAMP‐2B, V‐ATPase) and resident lumenal enzymes (Cathepsin B, Cathepsin D, Cathepsin L) (Figure 4C)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:iaXeLGV1ftr7OnUqbeLPZGpJ7NI10090 rogid:oFAYLk0ZhXto2nbmx/lUdQc2EJM10090 intact-crc:E7A58C754C6C2571|rigid:mjP94ovRpppRodGMuDvzGR5l+r8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P10605 uniprotkb:P11438 intact:EBI-2924871|ensembl:ENSMUSP00000006235|uniprotkb:Q3UDW7 intact:EBI-987439|uniprotkb:Q62020|ensembl:ENSMUSP00000033824 psi-mi:catb_mouse(display_long)|uniprotkb:Ctsb(gene name)|psi-mi:Ctsb(display_short)|uniprotkb:Cathepsin B1(gene name synonym) psi-mi:lamp1_mouse(display_long)|uniprotkb:Lysosomal membrane glycoprotein A(gene name synonym)|uniprotkb:120 kDa lysosomal membrane glycoprotein(gene name synonym)|uniprotkb:LGP-120(gene name synonym)|uniprotkb:P2B(gene name synonym)|uniprotkb:CD107 antigen-like family member A(gene name synonym)|uniprotkb:Lamp1(gene name)|psi-mi:Lamp1(display_short)|uniprotkb:Lamp-1(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103449|imex:IM-22093-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSMUSG00000021939(gene)|ensembl:ENSMUST00000006235(transcript)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0005518"(collagen binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0006508"(proteolysis)|go:"GO:0006590"(thyroid hormone generation)|go:"GO:0008233"(peptidase activity)|go:"GO:0008234"(cysteine-type peptidase activity)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0030574"(collagen catabolic process)|go:"GO:0030984"(kininogen binding)|go:"GO:0042277"(peptide binding)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043394"(proteoglycan binding)|go:"GO:0043621"(protein self-association)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0046697"(decidualization)|go:"GO:0046718"(viral entry into host cell)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050790"(regulation of catalytic activity)|go:"GO:0051603"(proteolysis involved in cellular protein catabolic process)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0062023"(collagen-containing extracellular matrix)|go:"GO:0097067"(cellular response to thyroid hormone stimulus)|interpro:IPR000169(Peptidase, cysteine peptidase active site)|interpro:IPR000668(Peptidase C1A, papain C-terminal)|interpro:IPR012599(Peptidase C1A, propeptide)|interpro:IPR025660|interpro:IPR025661|go:"GO:0030855"(epithelial cell differentiation)|mint:P10605|reactome:R-MMU-1442490|reactome:R-MMU-1679131|reactome:R-MMU-2022090|reactome:R-MMU-2132295|reactome:R-MMU-6798695|interpro:IPR038765|refseq:NP_031824.1 ensembl:ENSMUSG00000031447(gene)|ensembl:ENSMUST00000033824(transcript)|go:"GO:0000421"(autophagosome membrane)|go:"GO:0005737"(cytoplasm)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005768"(endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005771"(multivesicular body)|go:"GO:0005773"(vacuole)|refseq:NP_034814.2|go:"GO:0005886"(plasma membrane)|go:"GO:0007283"(spermatogenesis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0010008"(endosome membrane)|go:"GO:0019899"(enzyme binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030425"(dendrite)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031902"(late endosome membrane)|go:"GO:0031982"(vesicle)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0044194"(cytolytic granule)|go:"GO:0044754"(autolysosome)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048102"(autophagic cell death)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050821"(protein stabilization)|go:"GO:0061474"(phagolysosome membrane)|go:"GO:0072594"(establishment of protein localization to organelle)|go:"GO:0005829"(cytosol)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0097208"(alveolar lamellar body)|go:"GO:0101004"(cytolytic granule membrane)|go:"GO:0140507"(granzyme-mediated programmed cell death signaling pathway)|go:"GO:1902513"(regulation of organelle transport along microtubule)|interpro:IPR002000("Lysosome-associated membrane glycoprotein (Lamp)/CD68")|interpro:IPR018134|mint:P11438|rcsb pdb:5GV0|reactome:R-MMU-6798695|refseq:NP_001304282.1 go:"GO:0005764"(lysosome) - - figure legend:f4c f5c|comment:"We next purified Lys from control and PI4KIIIβ(−) using a previously standardized protocol that allows for the isolation of a fraction highly enriched in secondary Lys (the use of floatation gradients made lysosomal isolation comparable in both groups independent of the changes in sedimentation behaviour observed in Supplementary Figure S5C (see also Supplementary data; Aniento et al, 1993; Cuervo et al, 1997; Bandyopadhyay et al, 2010; Koga et al, 2010). Immunoblot revealed a significant reduction in the levels of integral membrane proteins (LAMP1, LAMP‐2A, LAMP‐2B, V‐ATPase) and resident lumenal enzymes (Cathepsin B, Cathepsin D, Cathepsin L) (Figure 4C)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:iaXeLGV1ftr7OnUqbeLPZGpJ7NI10090 rogid:34kBKJCpWncY9gLqL2lEx3Yn01I10090 intact-crc:E7A58C754C6C2571|rigid:mjP94ovRpppRodGMuDvzGR5l+r8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P10605 uniprotkb:P17047-1 intact:EBI-2924871|ensembl:ENSMUSP00000006235|uniprotkb:Q3UDW7 intact:EBI-9103451|ensembl:ENSMUSP00000016678 psi-mi:catb_mouse(display_long)|uniprotkb:Ctsb(gene name)|psi-mi:Ctsb(display_short)|uniprotkb:Cathepsin B1(gene name synonym) psi-mi:p17047-1(display_long)|uniprotkb:Lamp2(gene name)|psi-mi:Lamp2(display_short)|uniprotkb:Lamp-2(gene name synonym)|uniprotkb:CD107 antigen-like family member B(gene name synonym)|uniprotkb:Lysosomal membrane glycoprotein type B(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103449|imex:IM-22093-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSMUSG00000021939(gene)|ensembl:ENSMUST00000006235(transcript)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0005518"(collagen binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0006508"(proteolysis)|go:"GO:0006590"(thyroid hormone generation)|go:"GO:0008233"(peptidase activity)|go:"GO:0008234"(cysteine-type peptidase activity)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0030574"(collagen catabolic process)|go:"GO:0030984"(kininogen binding)|go:"GO:0042277"(peptide binding)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043394"(proteoglycan binding)|go:"GO:0043621"(protein self-association)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0046697"(decidualization)|go:"GO:0046718"(viral entry into host cell)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050790"(regulation of catalytic activity)|go:"GO:0051603"(proteolysis involved in cellular protein catabolic process)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0062023"(collagen-containing extracellular matrix)|go:"GO:0097067"(cellular response to thyroid hormone stimulus)|interpro:IPR000169(Peptidase, cysteine peptidase active site)|interpro:IPR000668(Peptidase C1A, papain C-terminal)|interpro:IPR012599(Peptidase C1A, propeptide)|interpro:IPR025660|interpro:IPR025661|go:"GO:0030855"(epithelial cell differentiation)|mint:P10605|reactome:R-MMU-1442490|reactome:R-MMU-1679131|reactome:R-MMU-2022090|reactome:R-MMU-2132295|reactome:R-MMU-6798695|interpro:IPR038765|refseq:NP_031824.1 intact:EBI-1564749(isoform-parent)|ensembl:ENSMUSG00000016534(gene)|ensembl:ENSMUST00000016678(transcript) go:"GO:0005764"(lysosome) - - figure legend:f4c f5c|comment:"We next purified Lys from control and PI4KIIIβ(−) using a previously standardized protocol that allows for the isolation of a fraction highly enriched in secondary Lys (the use of floatation gradients made lysosomal isolation comparable in both groups independent of the changes in sedimentation behaviour observed in Supplementary Figure S5C (see also Supplementary data; Aniento et al, 1993; Cuervo et al, 1997; Bandyopadhyay et al, 2010; Koga et al, 2010). Immunoblot revealed a significant reduction in the levels of integral membrane proteins (LAMP1, LAMP‐2A, LAMP‐2B, V‐ATPase) and resident lumenal enzymes (Cathepsin B, Cathepsin D, Cathepsin L) (Figure 4C)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:iaXeLGV1ftr7OnUqbeLPZGpJ7NI10090 rogid:em1v9qxpoHR13ocQdAffj+zQSDE10090 intact-crc:E7A58C754C6C2571|rigid:mjP94ovRpppRodGMuDvzGR5l+r8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P10605 uniprotkb:P17047-2 intact:EBI-2924871|ensembl:ENSMUSP00000006235|uniprotkb:Q3UDW7 intact:EBI-9103441|ensembl:ENSMUSP00000074448 psi-mi:catb_mouse(display_long)|uniprotkb:Ctsb(gene name)|psi-mi:Ctsb(display_short)|uniprotkb:Cathepsin B1(gene name synonym) psi-mi:p17047-2(display_long)|uniprotkb:Lamp2(gene name)|psi-mi:Lamp2(display_short)|uniprotkb:Lamp-2(gene name synonym)|uniprotkb:CD107 antigen-like family member B(gene name synonym)|uniprotkb:Lysosomal membrane glycoprotein type B(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103449|imex:IM-22093-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSMUSG00000021939(gene)|ensembl:ENSMUST00000006235(transcript)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0005518"(collagen binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0006508"(proteolysis)|go:"GO:0006590"(thyroid hormone generation)|go:"GO:0008233"(peptidase activity)|go:"GO:0008234"(cysteine-type peptidase activity)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0030574"(collagen catabolic process)|go:"GO:0030984"(kininogen binding)|go:"GO:0042277"(peptide binding)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043394"(proteoglycan binding)|go:"GO:0043621"(protein self-association)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0046697"(decidualization)|go:"GO:0046718"(viral entry into host cell)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050790"(regulation of catalytic activity)|go:"GO:0051603"(proteolysis involved in cellular protein catabolic process)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0062023"(collagen-containing extracellular matrix)|go:"GO:0097067"(cellular response to thyroid hormone stimulus)|interpro:IPR000169(Peptidase, cysteine peptidase active site)|interpro:IPR000668(Peptidase C1A, papain C-terminal)|interpro:IPR012599(Peptidase C1A, propeptide)|interpro:IPR025660|interpro:IPR025661|go:"GO:0030855"(epithelial cell differentiation)|mint:P10605|reactome:R-MMU-1442490|reactome:R-MMU-1679131|reactome:R-MMU-2022090|reactome:R-MMU-2132295|reactome:R-MMU-6798695|interpro:IPR038765|refseq:NP_031824.1 intact:EBI-1564749(isoform-parent)|ensembl:ENSMUSG00000016534(gene)|ensembl:ENSMUST00000074913(transcript) go:"GO:0005764"(lysosome) - - figure legend:f4c f5c|comment:"We next purified Lys from control and PI4KIIIβ(−) using a previously standardized protocol that allows for the isolation of a fraction highly enriched in secondary Lys (the use of floatation gradients made lysosomal isolation comparable in both groups independent of the changes in sedimentation behaviour observed in Supplementary Figure S5C (see also Supplementary data; Aniento et al, 1993; Cuervo et al, 1997; Bandyopadhyay et al, 2010; Koga et al, 2010). Immunoblot revealed a significant reduction in the levels of integral membrane proteins (LAMP1, LAMP‐2A, LAMP‐2B, V‐ATPase) and resident lumenal enzymes (Cathepsin B, Cathepsin D, Cathepsin L) (Figure 4C)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:iaXeLGV1ftr7OnUqbeLPZGpJ7NI10090 rogid:fcNboO41110Cstl6RJ8yoqANSKM10090 intact-crc:E7A58C754C6C2571|rigid:mjP94ovRpppRodGMuDvzGR5l+r8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P10605 uniprotkb:Q91YH6 intact:EBI-2924871|ensembl:ENSMUSP00000006235|uniprotkb:Q3UDW7 intact:EBI-8365420|uniprotkb:Q6P6I3|uniprotkb:Q8C3L6|intact:MINT-4121894|ensembl:ENSMUSP00000006431 psi-mi:catb_mouse(display_long)|uniprotkb:Ctsb(gene name)|psi-mi:Ctsb(display_short)|uniprotkb:Cathepsin B1(gene name synonym) psi-mi:vatb1_mouse(display_long)|uniprotkb:Atp6v1b1(gene name)|psi-mi:Atp6v1b1(display_short)|uniprotkb:Atp6b1(gene name synonym)|uniprotkb:Endomembrane proton pump 58 kDa subunit(gene name synonym)|uniprotkb:Vacuolar proton pump subunit B 1(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103449|imex:IM-22093-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSMUSG00000021939(gene)|ensembl:ENSMUST00000006235(transcript)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0005518"(collagen binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0006508"(proteolysis)|go:"GO:0006590"(thyroid hormone generation)|go:"GO:0008233"(peptidase activity)|go:"GO:0008234"(cysteine-type peptidase activity)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0030574"(collagen catabolic process)|go:"GO:0030984"(kininogen binding)|go:"GO:0042277"(peptide binding)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043394"(proteoglycan binding)|go:"GO:0043621"(protein self-association)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0046697"(decidualization)|go:"GO:0046718"(viral entry into host cell)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050790"(regulation of catalytic activity)|go:"GO:0051603"(proteolysis involved in cellular protein catabolic process)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0062023"(collagen-containing extracellular matrix)|go:"GO:0097067"(cellular response to thyroid hormone stimulus)|interpro:IPR000169(Peptidase, cysteine peptidase active site)|interpro:IPR000668(Peptidase C1A, papain C-terminal)|interpro:IPR012599(Peptidase C1A, propeptide)|interpro:IPR025660|interpro:IPR025661|go:"GO:0030855"(epithelial cell differentiation)|mint:P10605|reactome:R-MMU-1442490|reactome:R-MMU-1679131|reactome:R-MMU-2022090|reactome:R-MMU-2132295|reactome:R-MMU-6798695|interpro:IPR038765|refseq:NP_031824.1 refseq:NP_598918.1|ensembl:ENSMUSG00000006269(gene)|ensembl:ENSMUST00000006431(transcript)|go:"GO:0000221"(vacuolar proton-transporting V-type ATPase, V1 domain)|go:"GO:0001503"(ossification)|go:"GO:0003091"(renal water homeostasis)|go:"GO:0003096"(renal sodium ion transport)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005902"(microvillus)|go:"GO:0006693"(prostaglandin metabolic process)|go:"GO:0006885"(regulation of pH)|go:"GO:0007588"(excretion)|go:"GO:0007605"(sensory perception of sound)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0010468"(regulation of gene expression)|go:"GO:0015078"(proton transmembrane transporter activity)|go:"GO:0016020"(membrane)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0016328"(lateral plasma membrane)|go:"GO:0016471"(vacuolar proton-transporting V-type ATPase complex)|go:"GO:0016787"(hydrolase activity)|go:"GO:0030534"(adult behavior)|go:"GO:0035812"(renal sodium excretion)|go:"GO:0042048"(olfactory behavior)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045851"(pH reduction)|go:"GO:0046034"(ATP metabolic process)|go:"GO:0055064"(chloride ion homeostasis)|go:"GO:0055074"(calcium ion homeostasis)|go:"GO:0055075"(potassium ion homeostasis)|go:"GO:0070072"(vacuolar proton-transporting V-type ATPase complex assembly)|go:"GO:0098850"(extrinsic component of synaptic vesicle membrane)|go:"GO:1902600"(proton transmembrane transport)|interpro:IPR000194(ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding)|interpro:IPR004100(ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal)|interpro:IPR005723(ATPase, V1 complex, subunit B)|interpro:IPR020003|interpro:IPR022879|interpro:IPR027417|mint:Q91YH6|reactome:R-MMU-1222556|reactome:R-MMU-77387|reactome:R-MMU-917977|reactome:R-MMU-9639288|reactome:R-MMU-983712 go:"GO:0005764"(lysosome) - comment:mint figure legend:f4c f5c|comment:"We next purified Lys from control and PI4KIIIβ(−) using a previously standardized protocol that allows for the isolation of a fraction highly enriched in secondary Lys (the use of floatation gradients made lysosomal isolation comparable in both groups independent of the changes in sedimentation behaviour observed in Supplementary Figure S5C (see also Supplementary data; Aniento et al, 1993; Cuervo et al, 1997; Bandyopadhyay et al, 2010; Koga et al, 2010). Immunoblot revealed a significant reduction in the levels of integral membrane proteins (LAMP1, LAMP‐2A, LAMP‐2B, V‐ATPase) and resident lumenal enzymes (Cathepsin B, Cathepsin D, Cathepsin L) (Figure 4C)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:iaXeLGV1ftr7OnUqbeLPZGpJ7NI10090 rogid:BN28VewxPJE0pRYUsD6ExW5+qzI10090 intact-crc:E7A58C754C6C2571|rigid:mjP94ovRpppRodGMuDvzGR5l+r8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P17046 uniprotkb:Q99M64 intact:EBI-916686|uniprotkb:F1LLX8|uniprotkb:Q6P6W1|ensembl:ENSRNOP00000000177 intact:EBI-8159368|ensembl:ENSRNOP00000019874|intact:MINT-8427652 psi-mi:lamp2_rat(display_long)|uniprotkb:Lysosomal membrane glycoprotein type B(gene name synonym)|uniprotkb:LGP-96(gene name synonym)|uniprotkb:LGP-110(gene name synonym)|uniprotkb:CD107 antigen-like family member B(gene name synonym)|uniprotkb:Lamp2(gene name)|psi-mi:Lamp2(display_short)|uniprotkb:Lamp-2(gene name synonym) psi-mi:p4k2a_rat(display_long)|uniprotkb:Pi4k2a(gene name)|psi-mi:Pi4k2a(display_short)|uniprotkb:55 kDa type II phosphatidylinositol 4-kinase(gene name synonym)|uniprotkb:Phosphatidylinositol 4-kinase type II-alpha(gene name synonym)|uniprotkb:Pi4kii(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103483|imex:IM-22093-4 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:2990|refseq:NP_058764.2|ensembl:ENSRNOG00000000164(gene)|ensembl:ENSRNOT00000000177(transcript)|go:"GO:0005615"(extracellular space)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005768"(endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005886"(plasma membrane)|go:"GO:0006605"(protein targeting)|go:"GO:0006914"(autophagy)|go:"GO:0009267"(cellular response to starvation)|go:"GO:0016020"(membrane)|go:"GO:0019899"(enzyme binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030670"(phagocytic vesicle membrane)|go:"GO:0031088"(platelet dense granule membrane)|go:"GO:0031647"(regulation of protein stability)|go:"GO:0031902"(late endosome membrane)|go:"GO:0044754"(autolysosome)|go:"GO:0046716"(muscle cell cellular homeostasis)|go:"GO:0050821"(protein stabilization)|go:"GO:0061684"(chaperone-mediated autophagy)|go:"GO:0061740"(protein targeting to lysosome involved in chaperone-mediated autophagy)|go:"GO:0061742"(chaperone-mediated autophagy translocation complex)|go:"GO:0070062"(extracellular exosome)|go:"GO:0072594"(establishment of protein localization to organelle)|go:"GO:0097352"(autophagosome maturation)|go:"GO:0097637"(integral component of autophagosome membrane)|go:"GO:1905146"(lysosomal protein catabolic process)|go:"GO:1990836"(lysosomal matrix)|interpro:IPR002000("Lysosome-associated membrane glycoprotein (Lamp)/CD68")|interpro:IPR018134|mint:P17046|reactome:R-RNO-114608|reactome:R-RNO-6798695 ensembl:ENSRNOT00000019874(transcript)|go:"GO:0002561"(basophil degranulation)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006661"(phosphatidylinositol biosynthetic process)|go:"GO:0007030"(Golgi organization)|go:"GO:0007032"(endosome organization)|go:"GO:0030425"(dendrite)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031083"(BLOC-1 complex)|go:"GO:0031224"(intrinsic component of membrane)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031901"(early endosome membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0035651"(AP-3 adaptor complex binding)|go:"GO:0035838"(growing cell tip)|go:"GO:0042734"(presynaptic membrane)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043204"(perikaryon)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045121"(membrane raft)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0070382"(exocytic vesicle)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR039756|reactome:R-RNO-1660499|reactome:R-RNO-1660514|reactome:R-RNO-1660516|ensembl:ENSRNOG00000014675(gene)|rgd:620485|refseq:NP_446187.1 go:"GO:0005764"(lysosome) - comment:mint figure legend:f5a|comment:"We next investigated whether PI4KIIIβ might regulate lysosomal tubulation by directly acting in this compartment. Immunoblot of different subcellular fractions from rat liver revealed that most of PI4KIIIβ is present in cytosol and the Golgi but that a fraction (20±9%) of this protein can be detected in Lys (Figure 5A)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2017/02/23 rogid:joix4CrGSiBu/eJb0/wfPM42/FE10116 rogid:qOUXOEheubSSUJtrOvRPpKF4zPw10116 intact-crc:4F2EFB3FEF604CFD|rigid:6CDuENO3rGNw9fJOB1Rc3IQuca8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P17046 uniprotkb:O08561 intact:EBI-916686|uniprotkb:F1LLX8|uniprotkb:Q6P6W1|ensembl:ENSRNOP00000000177 intact:EBI-9085347|ensembl:ENSRNOP00000039342 psi-mi:lamp2_rat(display_long)|uniprotkb:Lysosomal membrane glycoprotein type B(gene name synonym)|uniprotkb:LGP-96(gene name synonym)|uniprotkb:LGP-110(gene name synonym)|uniprotkb:CD107 antigen-like family member B(gene name synonym)|uniprotkb:Lamp2(gene name)|psi-mi:Lamp2(display_short)|uniprotkb:Lamp-2(gene name synonym) psi-mi:pi4kb_rat(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103483|imex:IM-22093-4 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:2990|refseq:NP_058764.2|ensembl:ENSRNOG00000000164(gene)|ensembl:ENSRNOT00000000177(transcript)|go:"GO:0005615"(extracellular space)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005768"(endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005886"(plasma membrane)|go:"GO:0006605"(protein targeting)|go:"GO:0006914"(autophagy)|go:"GO:0009267"(cellular response to starvation)|go:"GO:0016020"(membrane)|go:"GO:0019899"(enzyme binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030670"(phagocytic vesicle membrane)|go:"GO:0031088"(platelet dense granule membrane)|go:"GO:0031647"(regulation of protein stability)|go:"GO:0031902"(late endosome membrane)|go:"GO:0044754"(autolysosome)|go:"GO:0046716"(muscle cell cellular homeostasis)|go:"GO:0050821"(protein stabilization)|go:"GO:0061684"(chaperone-mediated autophagy)|go:"GO:0061740"(protein targeting to lysosome involved in chaperone-mediated autophagy)|go:"GO:0061742"(chaperone-mediated autophagy translocation complex)|go:"GO:0070062"(extracellular exosome)|go:"GO:0072594"(establishment of protein localization to organelle)|go:"GO:0097352"(autophagosome maturation)|go:"GO:0097637"(integral component of autophagosome membrane)|go:"GO:1905146"(lysosomal protein catabolic process)|go:"GO:1990836"(lysosomal matrix)|interpro:IPR002000("Lysosome-associated membrane glycoprotein (Lamp)/CD68")|interpro:IPR018134|mint:P17046|reactome:R-RNO-114608|reactome:R-RNO-6798695 rgd:621214|refseq:NP_112345.1|ensembl:ENSRNOG00000021024(gene)|ensembl:ENSRNOT00000040501(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-RNO-1660514 go:"GO:0005764"(lysosome) - - figure legend:f5a|comment:"We next investigated whether PI4KIIIβ might regulate lysosomal tubulation by directly acting in this compartment. Immunoblot of different subcellular fractions from rat liver revealed that most of PI4KIIIβ is present in cytosol and the Golgi but that a fraction (20±9%) of this protein can be detected in Lys (Figure 5A)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2017/02/23 rogid:joix4CrGSiBu/eJb0/wfPM42/FE10116 rogid:yTl5CFz9dSADvKpr1FLFVoTg6mA10116 intact-crc:4F2EFB3FEF604CFD|rigid:6CDuENO3rGNw9fJOB1Rc3IQuca8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P17046 uniprotkb:Q6P6T6 intact:EBI-916686|uniprotkb:F1LLX8|uniprotkb:Q6P6W1|ensembl:ENSRNOP00000000177 intact:EBI-9103485|ensembl:ENSRNOP00000027407 psi-mi:lamp2_rat(display_long)|uniprotkb:Lysosomal membrane glycoprotein type B(gene name synonym)|uniprotkb:LGP-96(gene name synonym)|uniprotkb:LGP-110(gene name synonym)|uniprotkb:CD107 antigen-like family member B(gene name synonym)|uniprotkb:Lamp2(gene name)|psi-mi:Lamp2(display_short)|uniprotkb:Lamp-2(gene name synonym) psi-mi:q6p6t6_rat(display_long)|uniprotkb:Ctsd(gene name)|psi-mi:Ctsd(display_short)|uniprotkb:rCG_47835(orf name) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103483|imex:IM-22093-4 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:2990|refseq:NP_058764.2|ensembl:ENSRNOG00000000164(gene)|ensembl:ENSRNOT00000000177(transcript)|go:"GO:0005615"(extracellular space)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005768"(endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005886"(plasma membrane)|go:"GO:0006605"(protein targeting)|go:"GO:0006914"(autophagy)|go:"GO:0009267"(cellular response to starvation)|go:"GO:0016020"(membrane)|go:"GO:0019899"(enzyme binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030670"(phagocytic vesicle membrane)|go:"GO:0031088"(platelet dense granule membrane)|go:"GO:0031647"(regulation of protein stability)|go:"GO:0031902"(late endosome membrane)|go:"GO:0044754"(autolysosome)|go:"GO:0046716"(muscle cell cellular homeostasis)|go:"GO:0050821"(protein stabilization)|go:"GO:0061684"(chaperone-mediated autophagy)|go:"GO:0061740"(protein targeting to lysosome involved in chaperone-mediated autophagy)|go:"GO:0061742"(chaperone-mediated autophagy translocation complex)|go:"GO:0070062"(extracellular exosome)|go:"GO:0072594"(establishment of protein localization to organelle)|go:"GO:0097352"(autophagosome maturation)|go:"GO:0097637"(integral component of autophagosome membrane)|go:"GO:1905146"(lysosomal protein catabolic process)|go:"GO:1990836"(lysosomal matrix)|interpro:IPR002000("Lysosome-associated membrane glycoprotein (Lamp)/CD68")|interpro:IPR018134|mint:P17046|reactome:R-RNO-114608|reactome:R-RNO-6798695 refseq:NP_599161.2|rgd:621511|ensembl:ENSRNOG00000020206(gene)|ensembl:ENSRNOT00000027407(transcript)|go:"GO:0004190"(aspartic-type endopeptidase activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005764"(lysosome)|interpro:IPR001461(Peptidase A1)|interpro:IPR001969(Peptidase aspartic, active site)|interpro:IPR012848(Propeptide, peptidase A1)|interpro:IPR021109|interpro:IPR033121|interpro:IPR033144 go:"GO:0005764"(lysosome) - - figure legend:f5a|comment:"We next investigated whether PI4KIIIβ might regulate lysosomal tubulation by directly acting in this compartment. Immunoblot of different subcellular fractions from rat liver revealed that most of PI4KIIIβ is present in cytosol and the Golgi but that a fraction (20±9%) of this protein can be detected in Lys (Figure 5A)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2017/02/23 rogid:joix4CrGSiBu/eJb0/wfPM42/FE10116 rogid:vxL58oQxUTyqUnKRw7XvV7m0xl010116 intact-crc:4F2EFB3FEF604CFD|rigid:6CDuENO3rGNw9fJOB1Rc3IQuca8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62839 uniprotkb:Q99M64 intact:EBI-618335|uniprotkb:F1LSS0 intact:EBI-8159368|ensembl:ENSRNOP00000019874|intact:MINT-8427652 psi-mi:goga2_rat(display_long)|uniprotkb:Golga2(gene name)|psi-mi:Golga2(display_short)|uniprotkb:130 kDa cis-Golgi matrix protein(gene name synonym) psi-mi:p4k2a_rat(display_long)|uniprotkb:Pi4k2a(gene name)|psi-mi:Pi4k2a(display_short)|uniprotkb:55 kDa type II phosphatidylinositol 4-kinase(gene name synonym)|uniprotkb:Phosphatidylinositol 4-kinase type II-alpha(gene name synonym)|uniprotkb:Pi4kii(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103507|imex:IM-22093-5 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR043937|interpro:IPR043976|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000922"(spindle pole)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005874"(microtubule)|go:"GO:0006486"(protein glycosylation)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0007020"(microtubule nucleation)|go:"GO:0007030"(Golgi organization)|go:"GO:0008017"(microtubule binding)|go:"GO:0008356"(asymmetric cell division)|go:"GO:0010507"(negative regulation of autophagy)|go:"GO:0015031"(protein transport)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030134"(COPII-coated ER to Golgi transport vesicle)|go:"GO:0032091"(negative regulation of protein binding)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051225"(spindle assembly)|go:"GO:0051289"(protein homotetramerization)|go:"GO:0051645"(Golgi localization)|go:"GO:0060050"(positive regulation of protein glycosylation)|go:"GO:0061676"(importin-alpha family protein binding)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090161"(Golgi ribbon formation)|go:"GO:0090166"(Golgi disassembly)|go:"GO:0090306"(meiotic spindle assembly)|go:"GO:0090307"(mitotic spindle assembly)|reactome:R-RNO-162658|reactome:R-RNO-204005|go:"GO:0007098"(centrosome cycle)|reactome:R-RNO-6807878|reactome:R-RNO-9013148|reactome:R-RNO-9013405|rgd:620282|refseq:NP_072118.2|refseq:XP_006234060.1|interpro:IPR024858|mint:Q62839 ensembl:ENSRNOT00000019874(transcript)|go:"GO:0002561"(basophil degranulation)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006661"(phosphatidylinositol biosynthetic process)|go:"GO:0007030"(Golgi organization)|go:"GO:0007032"(endosome organization)|go:"GO:0030425"(dendrite)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031083"(BLOC-1 complex)|go:"GO:0031224"(intrinsic component of membrane)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031901"(early endosome membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0035651"(AP-3 adaptor complex binding)|go:"GO:0035838"(growing cell tip)|go:"GO:0042734"(presynaptic membrane)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043204"(perikaryon)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045121"(membrane raft)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0070382"(exocytic vesicle)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR039756|reactome:R-RNO-1660499|reactome:R-RNO-1660514|reactome:R-RNO-1660516|ensembl:ENSRNOG00000014675(gene)|rgd:620485|refseq:NP_446187.1 go:"GO:0005794"(Golgi apparatus) - comment:mint figure legend:f5a|comment:"We next investigated whether PI4KIIIβ might regulate lysosomal tubulation by directly acting in this compartment. Immunoblot of different subcellular fractions from rat liver revealed that most of PI4KIIIβ is present in cytosol and the Golgi but that a fraction (20±9%) of this protein can be detected in Lys (Figure 5A)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2015/11/10 rogid:zW0U11na/96FACXJccRSnFPMte010116 rogid:qOUXOEheubSSUJtrOvRPpKF4zPw10116 intact-crc:F7F2283574035E65|rigid:U1xwo2NCQuUCxziK5y5C0yX2vN8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62839 uniprotkb:O08561 intact:EBI-618335|uniprotkb:F1LSS0 intact:EBI-9085347|ensembl:ENSRNOP00000039342 psi-mi:goga2_rat(display_long)|uniprotkb:Golga2(gene name)|psi-mi:Golga2(display_short)|uniprotkb:130 kDa cis-Golgi matrix protein(gene name synonym) psi-mi:pi4kb_rat(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103507|imex:IM-22093-5 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR043937|interpro:IPR043976|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000922"(spindle pole)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005874"(microtubule)|go:"GO:0006486"(protein glycosylation)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0007020"(microtubule nucleation)|go:"GO:0007030"(Golgi organization)|go:"GO:0008017"(microtubule binding)|go:"GO:0008356"(asymmetric cell division)|go:"GO:0010507"(negative regulation of autophagy)|go:"GO:0015031"(protein transport)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030134"(COPII-coated ER to Golgi transport vesicle)|go:"GO:0032091"(negative regulation of protein binding)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051225"(spindle assembly)|go:"GO:0051289"(protein homotetramerization)|go:"GO:0051645"(Golgi localization)|go:"GO:0060050"(positive regulation of protein glycosylation)|go:"GO:0061676"(importin-alpha family protein binding)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090161"(Golgi ribbon formation)|go:"GO:0090166"(Golgi disassembly)|go:"GO:0090306"(meiotic spindle assembly)|go:"GO:0090307"(mitotic spindle assembly)|reactome:R-RNO-162658|reactome:R-RNO-204005|go:"GO:0007098"(centrosome cycle)|reactome:R-RNO-6807878|reactome:R-RNO-9013148|reactome:R-RNO-9013405|rgd:620282|refseq:NP_072118.2|refseq:XP_006234060.1|interpro:IPR024858|mint:Q62839 rgd:621214|refseq:NP_112345.1|ensembl:ENSRNOG00000021024(gene)|ensembl:ENSRNOT00000040501(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-RNO-1660514 go:"GO:0005794"(Golgi apparatus) - - figure legend:f5a|comment:"We next investigated whether PI4KIIIβ might regulate lysosomal tubulation by directly acting in this compartment. Immunoblot of different subcellular fractions from rat liver revealed that most of PI4KIIIβ is present in cytosol and the Golgi but that a fraction (20±9%) of this protein can be detected in Lys (Figure 5A)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2015/11/10 rogid:zW0U11na/96FACXJccRSnFPMte010116 rogid:yTl5CFz9dSADvKpr1FLFVoTg6mA10116 intact-crc:F7F2283574035E65|rigid:U1xwo2NCQuUCxziK5y5C0yX2vN8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P22892 uniprotkb:O54774 intact:EBI-1040262|ensembl:ENSMUSP00000034171 intact:EBI-775247|ensembl:ENSMUSP00000020420 psi-mi:ap1g1_mouse(display_long)|uniprotkb:Ap1g1(gene name)|psi-mi:Ap1g1(display_short)|uniprotkb:Adtg(gene name synonym)|uniprotkb:Clapg1(gene name synonym)|uniprotkb:Adaptor protein complex AP-1 subunit gamma-1(gene name synonym)|uniprotkb:Golgi adaptor HA1/AP1 adaptin subunit gamma-1(gene name synonym)|uniprotkb:Gamma1-adaptin(gene name synonym)|uniprotkb:Gamma-adaptin(gene name synonym)|uniprotkb:Clathrin assembly protein complex 1 gamma-1 large chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 1 subunit gamma-1(gene name synonym) psi-mi:ap3d1_mouse(display_long)|uniprotkb:AP-3 complex subunit delta(gene name synonym)|uniprotkb:Delta-adaptin(gene name synonym)|uniprotkb:Ap3d1(gene name)|psi-mi:Ap3d1(display_short)|uniprotkb:Ap3d(gene name synonym)|uniprotkb:Adaptor-related protein complex 3 subunit delta-1(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103779|imex:IM-22093-11 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001288140.1|ensembl:ENSMUSG00000031731(gene)|ensembl:ENSMUST00000034171(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0005518"(collagen binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019894"(kinesin binding)|go:"GO:0030121"(AP-1 adaptor complex)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030742"(GTP-dependent protein binding)|go:"GO:0031267"(small GTPase binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035646"(endosome to melanosome transport)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055037"(recycling endosome)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0140312"(cargo adaptor activity)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR008152(Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain)|interpro:IPR008153(Clathrin adaptor, gamma-adaptin, appendage)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR013041(Clathrin/coatomer adaptor, adaptin-like, appendage, Ig-like subdomain)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017107(Adaptor protein complex AP-1, gamma subunit)|mint:P22892|rcsb pdb:1GYU|rcsb pdb:1GYV|rcsb pdb:1GYW|rcsb pdb:1W63|rcsb pdb:2A7B|rcsb pdb:3ZY7|rcsb pdb:4HMY|rcsb pdb:4P6Z|rcsb pdb:6CM9|rcsb pdb:6CRI|rcsb pdb:6D83|rcsb pdb:6D84|rcsb pdb:6DFF|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|go:"GO:0015630"(microtubule cytoskeleton) refseq:NP_031486.1|dip:DIP-32163N|ensembl:ENSMUSG00000020198(gene)|ensembl:ENSMUST00000020420(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006829"(zinc ion transport)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0008089"(anterograde axonal transport)|go:"GO:0010008"(endosome membrane)|go:"GO:0016182"(synaptic vesicle budding from endosome)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0030123"(AP-3 adaptor complex)|go:"GO:0030424"(axon)|go:"GO:0033365"(protein localization to organelle)|go:"GO:0035646"(endosome to melanosome transport)|go:"GO:0043195"(terminal bouton)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0048007"(antigen processing and presentation, exogenous lipid antigen via MHC class Ib)|go:"GO:0048490"(anterograde synaptic vesicle transport)|go:"GO:0048499"(synaptic vesicle membrane organization)|go:"GO:0051138"(positive regulation of NK T cell differentiation)|go:"GO:0061088"(regulation of sequestering of zinc ion)|go:"GO:0072657"(protein localization to membrane)|go:"GO:0098793"(presynapse)|go:"GO:0098794"(postsynapse)|go:"GO:0098830"(presynaptic endosome)|go:"GO:0098943"(neurotransmitter receptor transport, postsynaptic endosome to lysosome)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099003"(vesicle-mediated transport in synapse)|go:"GO:1904115"(axon cytoplasm)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR010474(Bovine leukaemia virus receptor)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017105(Adaptor protein complex AP-3, delta subunit)|mint:O54774 go:"GO:0005829"(cytosol) - - figure legend:f6f|comment:"Comparison of the association of each of these components with carriers isolated from control and PI4KIIIβ(−) cells (Figure 6F) revealed that loss of PI4KIIIβ led to markedly higher association of clathrin (∼3.5‐fold), the adaptor AP‐2 (∼2.8‐fold) and the plus‐end directed motor Kif13b (>5‐fold) to these vesicles and to higher content of lysosomal constituents (LAMP1 and Cath D shown here) (Figure 6F). The increase in AP‐2 was in clear contrast to the pronounced (∼3‐fold) decrease in AP‐3. Although more discrete, we also observed enhanced association of Rab7, Rab9 and Rab11 in the PI4KIIIβ‐defective carriers (Figure 6F)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:VeKvrfk0rSWIQfepmlhaOm293MI10090 rogid:q83u6ORB4zYNuQihE4PI1rhvSkY10090 intact-crc:EB3F9D66BC246A54|rigid:nIgmXqpW0Tg1sjQu7pAQl7gZ7ns false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P22892 uniprotkb:P18242 intact:EBI-1040262|ensembl:ENSMUSP00000034171 intact:EBI-738562|ensembl:ENSMUSP00000121203 psi-mi:ap1g1_mouse(display_long)|uniprotkb:Ap1g1(gene name)|psi-mi:Ap1g1(display_short)|uniprotkb:Adtg(gene name synonym)|uniprotkb:Clapg1(gene name synonym)|uniprotkb:Adaptor protein complex AP-1 subunit gamma-1(gene name synonym)|uniprotkb:Golgi adaptor HA1/AP1 adaptin subunit gamma-1(gene name synonym)|uniprotkb:Gamma1-adaptin(gene name synonym)|uniprotkb:Gamma-adaptin(gene name synonym)|uniprotkb:Clathrin assembly protein complex 1 gamma-1 large chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 1 subunit gamma-1(gene name synonym) psi-mi:catd_mouse(display_long)|uniprotkb:Ctsd(gene name)|psi-mi:Ctsd(display_short) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103779|imex:IM-22093-11 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001288140.1|ensembl:ENSMUSG00000031731(gene)|ensembl:ENSMUST00000034171(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0005518"(collagen binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019894"(kinesin binding)|go:"GO:0030121"(AP-1 adaptor complex)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030742"(GTP-dependent protein binding)|go:"GO:0031267"(small GTPase binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035646"(endosome to melanosome transport)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055037"(recycling endosome)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0140312"(cargo adaptor activity)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR008152(Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain)|interpro:IPR008153(Clathrin adaptor, gamma-adaptin, appendage)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR013041(Clathrin/coatomer adaptor, adaptin-like, appendage, Ig-like subdomain)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017107(Adaptor protein complex AP-1, gamma subunit)|mint:P22892|rcsb pdb:1GYU|rcsb pdb:1GYV|rcsb pdb:1GYW|rcsb pdb:1W63|rcsb pdb:2A7B|rcsb pdb:3ZY7|rcsb pdb:4HMY|rcsb pdb:4P6Z|rcsb pdb:6CM9|rcsb pdb:6CRI|rcsb pdb:6D83|rcsb pdb:6D84|rcsb pdb:6DFF|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|go:"GO:0015630"(microtubule cytoskeleton) refseq:NP_034113.1|go:"GO:0042159"(lipoprotein catabolic process)|go:"GO:0042277"(peptide binding)|go:"GO:0042470"(melanosome)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0045121"(membrane raft)|go:"GO:0062023"(collagen-containing extracellular matrix)|go:"GO:0070001"(aspartic-type peptidase activity)|go:"GO:0070201"(regulation of establishment of protein localization)|interpro:IPR001461(Peptidase A1)|interpro:IPR001969(Peptidase aspartic, active site)|interpro:IPR012848(Propeptide, peptidase A1)|go:"GO:0004190"(aspartic-type endopeptidase activity)|interpro:IPR021109|interpro:IPR033121|interpro:IPR033144|mint:P18242|go:"GO:0005615"(extracellular space)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|reactome:R-MMU-1442490|go:"GO:0006508"(proteolysis)|go:"GO:0008233"(peptidase activity)|reactome:R-MMU-2022377|reactome:R-MMU-2132295|reactome:R-MMU-6798695|go:"GO:0010008"(endosome membrane)|go:"GO:0016787"(hydrolase activity)|ensembl:ENSMUSG00000007891(gene)|ensembl:ENSMUST00000151120(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0004175"(endopeptidase activity) go:"GO:0005829"(cytosol) - - figure legend:f6f|comment:"Comparison of the association of each of these components with carriers isolated from control and PI4KIIIβ(−) cells (Figure 6F) revealed that loss of PI4KIIIβ led to markedly higher association of clathrin (∼3.5‐fold), the adaptor AP‐2 (∼2.8‐fold) and the plus‐end directed motor Kif13b (>5‐fold) to these vesicles and to higher content of lysosomal constituents (LAMP1 and Cath D shown here) (Figure 6F). The increase in AP‐2 was in clear contrast to the pronounced (∼3‐fold) decrease in AP‐3. Although more discrete, we also observed enhanced association of Rab7, Rab9 and Rab11 in the PI4KIIIβ‐defective carriers (Figure 6F)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:VeKvrfk0rSWIQfepmlhaOm293MI10090 rogid:PrpcvWCAFSmTF9ECBCB/zMV6d9M10090 intact-crc:EB3F9D66BC246A54|rigid:nIgmXqpW0Tg1sjQu7pAQl7gZ7ns false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P22892 uniprotkb:Q68FD5 intact:EBI-1040262|ensembl:ENSMUSP00000034171 intact:EBI-769168|ensembl:ENSMUSP00000099475 psi-mi:ap1g1_mouse(display_long)|uniprotkb:Ap1g1(gene name)|psi-mi:Ap1g1(display_short)|uniprotkb:Adtg(gene name synonym)|uniprotkb:Clapg1(gene name synonym)|uniprotkb:Adaptor protein complex AP-1 subunit gamma-1(gene name synonym)|uniprotkb:Golgi adaptor HA1/AP1 adaptin subunit gamma-1(gene name synonym)|uniprotkb:Gamma1-adaptin(gene name synonym)|uniprotkb:Gamma-adaptin(gene name synonym)|uniprotkb:Clathrin assembly protein complex 1 gamma-1 large chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 1 subunit gamma-1(gene name synonym) psi-mi:clh1_mouse(display_long)|uniprotkb:Cltc(gene name)|psi-mi:Cltc(display_short) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103779|imex:IM-22093-11 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001288140.1|ensembl:ENSMUSG00000031731(gene)|ensembl:ENSMUST00000034171(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0005518"(collagen binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019894"(kinesin binding)|go:"GO:0030121"(AP-1 adaptor complex)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030742"(GTP-dependent protein binding)|go:"GO:0031267"(small GTPase binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035646"(endosome to melanosome transport)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055037"(recycling endosome)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0140312"(cargo adaptor activity)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR008152(Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain)|interpro:IPR008153(Clathrin adaptor, gamma-adaptin, appendage)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR013041(Clathrin/coatomer adaptor, adaptin-like, appendage, Ig-like subdomain)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017107(Adaptor protein complex AP-1, gamma subunit)|mint:P22892|rcsb pdb:1GYU|rcsb pdb:1GYV|rcsb pdb:1GYW|rcsb pdb:1W63|rcsb pdb:2A7B|rcsb pdb:3ZY7|rcsb pdb:4HMY|rcsb pdb:4P6Z|rcsb pdb:6CM9|rcsb pdb:6CRI|rcsb pdb:6D83|rcsb pdb:6D84|rcsb pdb:6DFF|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|go:"GO:0015630"(microtubule cytoskeleton) refseq:NP_001003908.1|dip:DIP-31947N|ensembl:ENSMUSG00000047126(gene)|ensembl:ENSMUST00000103186(transcript)|go:"GO:0000278"(mitotic cell cycle)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0005198"(structural molecule activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0005768"(endosome)|go:"GO:0005819"(spindle)|go:"GO:0005829"(cytosol)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006914"(autophagy)|go:"GO:0007030"(Golgi organization)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016020"(membrane)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030117"(membrane coat)|go:"GO:0030118"(clathrin coat)|go:"GO:0030130"(clathrin coat of trans-Golgi network vesicle)|go:"GO:0030132"(clathrin coat of coated pit)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030315"(T-tubule)|go:"GO:0030506"(ankyrin binding)|go:"GO:0031072"(heat shock protein binding)|go:"GO:0031523"(Myb complex)|go:"GO:0031623"(receptor internalization)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033572"(transferrin transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0042277"(peptide binding)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043209"(myelin sheath)|go:"GO:0045334"(clathrin-coated endocytic vesicle)|go:"GO:0048268"(clathrin coat assembly)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0050750"(low-density lipoprotein particle receptor binding)|go:"GO:0051301"(cell division)|go:"GO:0060236"(regulation of mitotic spindle organization)|go:"GO:0071439"(clathrin complex)|go:"GO:0072583"(clathrin-dependent endocytosis)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090307"(mitotic spindle assembly)|go:"GO:0097718"(disordered domain specific binding)|go:"GO:0098684"(photoreceptor ribbon synapse)|go:"GO:0098835"(presynaptic endocytic zone membrane)|go:"GO:0098850"(extrinsic component of synaptic vesicle membrane)|go:"GO:0150093"(amyloid-beta clearance by transcytosis)|go:"GO:1900126"(negative regulation of hyaluronan biosynthetic process)|go:"GO:1903077"(negative regulation of protein localization to plasma membrane)|go:"GO:1990381"(ubiquitin-specific protease binding)|go:"GO:1990498"(mitotic spindle microtubule)|interpro:IPR000547(Clathrin, heavy chain/VPS, 7-fold repeat)|interpro:IPR011990(Tetratricopeptide-like helical)|interpro:IPR015348(Clathrin, heavy chain, linker, core motif)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR016025(Clathrin, heavy chain, linker and propeller)|interpro:IPR016341(Clathrin, heavy chain)|interpro:IPR022365|mint:Q68FD5|rcsb pdb:5ODS|reactome:R-MMU-177504|reactome:R-MMU-190873|reactome:R-MMU-196025|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|reactome:R-MMU-437239|reactome:R-MMU-5099900|reactome:R-MMU-5140745|reactome:R-MMU-8856825|reactome:R-MMU-8856828|reactome:R-MMU-8866427|reactome:R-MMU-8964038|reactome:R-MMU-9013420|reactome:R-MMU-9013424|go:"GO:0032051"(clathrin light chain binding) go:"GO:0005829"(cytosol) - - figure legend:f6f|comment:"Comparison of the association of each of these components with carriers isolated from control and PI4KIIIβ(−) cells (Figure 6F) revealed that loss of PI4KIIIβ led to markedly higher association of clathrin (∼3.5‐fold), the adaptor AP‐2 (∼2.8‐fold) and the plus‐end directed motor Kif13b (>5‐fold) to these vesicles and to higher content of lysosomal constituents (LAMP1 and Cath D shown here) (Figure 6F). The increase in AP‐2 was in clear contrast to the pronounced (∼3‐fold) decrease in AP‐3. Although more discrete, we also observed enhanced association of Rab7, Rab9 and Rab11 in the PI4KIIIβ‐defective carriers (Figure 6F)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:VeKvrfk0rSWIQfepmlhaOm293MI10090 rogid:iHg/TOG2rWePqtsEmuc/szn/chI10090 intact-crc:EB3F9D66BC246A54|rigid:nIgmXqpW0Tg1sjQu7pAQl7gZ7ns false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P22892 uniprotkb:P11438 intact:EBI-1040262|ensembl:ENSMUSP00000034171 intact:EBI-987439|uniprotkb:Q62020|ensembl:ENSMUSP00000033824 psi-mi:ap1g1_mouse(display_long)|uniprotkb:Ap1g1(gene name)|psi-mi:Ap1g1(display_short)|uniprotkb:Adtg(gene name synonym)|uniprotkb:Clapg1(gene name synonym)|uniprotkb:Adaptor protein complex AP-1 subunit gamma-1(gene name synonym)|uniprotkb:Golgi adaptor HA1/AP1 adaptin subunit gamma-1(gene name synonym)|uniprotkb:Gamma1-adaptin(gene name synonym)|uniprotkb:Gamma-adaptin(gene name synonym)|uniprotkb:Clathrin assembly protein complex 1 gamma-1 large chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 1 subunit gamma-1(gene name synonym) psi-mi:lamp1_mouse(display_long)|uniprotkb:Lysosomal membrane glycoprotein A(gene name synonym)|uniprotkb:120 kDa lysosomal membrane glycoprotein(gene name synonym)|uniprotkb:LGP-120(gene name synonym)|uniprotkb:P2B(gene name synonym)|uniprotkb:CD107 antigen-like family member A(gene name synonym)|uniprotkb:Lamp1(gene name)|psi-mi:Lamp1(display_short)|uniprotkb:Lamp-1(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103779|imex:IM-22093-11 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001288140.1|ensembl:ENSMUSG00000031731(gene)|ensembl:ENSMUST00000034171(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0005518"(collagen binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019894"(kinesin binding)|go:"GO:0030121"(AP-1 adaptor complex)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030742"(GTP-dependent protein binding)|go:"GO:0031267"(small GTPase binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035646"(endosome to melanosome transport)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055037"(recycling endosome)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0140312"(cargo adaptor activity)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR008152(Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain)|interpro:IPR008153(Clathrin adaptor, gamma-adaptin, appendage)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR013041(Clathrin/coatomer adaptor, adaptin-like, appendage, Ig-like subdomain)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017107(Adaptor protein complex AP-1, gamma subunit)|mint:P22892|rcsb pdb:1GYU|rcsb pdb:1GYV|rcsb pdb:1GYW|rcsb pdb:1W63|rcsb pdb:2A7B|rcsb pdb:3ZY7|rcsb pdb:4HMY|rcsb pdb:4P6Z|rcsb pdb:6CM9|rcsb pdb:6CRI|rcsb pdb:6D83|rcsb pdb:6D84|rcsb pdb:6DFF|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|go:"GO:0015630"(microtubule cytoskeleton) ensembl:ENSMUSG00000031447(gene)|ensembl:ENSMUST00000033824(transcript)|go:"GO:0000421"(autophagosome membrane)|go:"GO:0005737"(cytoplasm)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005768"(endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005771"(multivesicular body)|go:"GO:0005773"(vacuole)|refseq:NP_034814.2|go:"GO:0005886"(plasma membrane)|go:"GO:0007283"(spermatogenesis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0010008"(endosome membrane)|go:"GO:0019899"(enzyme binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030425"(dendrite)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031902"(late endosome membrane)|go:"GO:0031982"(vesicle)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0044194"(cytolytic granule)|go:"GO:0044754"(autolysosome)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048102"(autophagic cell death)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050821"(protein stabilization)|go:"GO:0061474"(phagolysosome membrane)|go:"GO:0072594"(establishment of protein localization to organelle)|go:"GO:0005829"(cytosol)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0097208"(alveolar lamellar body)|go:"GO:0101004"(cytolytic granule membrane)|go:"GO:0140507"(granzyme-mediated programmed cell death signaling pathway)|go:"GO:1902513"(regulation of organelle transport along microtubule)|interpro:IPR002000("Lysosome-associated membrane glycoprotein (Lamp)/CD68")|interpro:IPR018134|mint:P11438|rcsb pdb:5GV0|reactome:R-MMU-6798695|refseq:NP_001304282.1 go:"GO:0005829"(cytosol) - - figure legend:f6f|comment:"Comparison of the association of each of these components with carriers isolated from control and PI4KIIIβ(−) cells (Figure 6F) revealed that loss of PI4KIIIβ led to markedly higher association of clathrin (∼3.5‐fold), the adaptor AP‐2 (∼2.8‐fold) and the plus‐end directed motor Kif13b (>5‐fold) to these vesicles and to higher content of lysosomal constituents (LAMP1 and Cath D shown here) (Figure 6F). The increase in AP‐2 was in clear contrast to the pronounced (∼3‐fold) decrease in AP‐3. Although more discrete, we also observed enhanced association of Rab7, Rab9 and Rab11 in the PI4KIIIβ‐defective carriers (Figure 6F)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:VeKvrfk0rSWIQfepmlhaOm293MI10090 rogid:34kBKJCpWncY9gLqL2lEx3Yn01I10090 intact-crc:EB3F9D66BC246A54|rigid:nIgmXqpW0Tg1sjQu7pAQl7gZ7ns false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P22892 uniprotkb:Q8BKC8 intact:EBI-1040262|ensembl:ENSMUSP00000034171 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 psi-mi:ap1g1_mouse(display_long)|uniprotkb:Ap1g1(gene name)|psi-mi:Ap1g1(display_short)|uniprotkb:Adtg(gene name synonym)|uniprotkb:Clapg1(gene name synonym)|uniprotkb:Adaptor protein complex AP-1 subunit gamma-1(gene name synonym)|uniprotkb:Golgi adaptor HA1/AP1 adaptin subunit gamma-1(gene name synonym)|uniprotkb:Gamma1-adaptin(gene name synonym)|uniprotkb:Gamma-adaptin(gene name synonym)|uniprotkb:Clathrin assembly protein complex 1 gamma-1 large chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 1 subunit gamma-1(gene name synonym) psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103779|imex:IM-22093-11 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001288140.1|ensembl:ENSMUSG00000031731(gene)|ensembl:ENSMUST00000034171(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0005518"(collagen binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019894"(kinesin binding)|go:"GO:0030121"(AP-1 adaptor complex)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030742"(GTP-dependent protein binding)|go:"GO:0031267"(small GTPase binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035646"(endosome to melanosome transport)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055037"(recycling endosome)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0140312"(cargo adaptor activity)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR008152(Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain)|interpro:IPR008153(Clathrin adaptor, gamma-adaptin, appendage)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR013041(Clathrin/coatomer adaptor, adaptin-like, appendage, Ig-like subdomain)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017107(Adaptor protein complex AP-1, gamma subunit)|mint:P22892|rcsb pdb:1GYU|rcsb pdb:1GYV|rcsb pdb:1GYW|rcsb pdb:1W63|rcsb pdb:2A7B|rcsb pdb:3ZY7|rcsb pdb:4HMY|rcsb pdb:4P6Z|rcsb pdb:6CM9|rcsb pdb:6CRI|rcsb pdb:6D83|rcsb pdb:6D84|rcsb pdb:6DFF|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|go:"GO:0015630"(microtubule cytoskeleton) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 go:"GO:0005829"(cytosol) - - figure legend:f6f|comment:"Comparison of the association of each of these components with carriers isolated from control and PI4KIIIβ(−) cells (Figure 6F) revealed that loss of PI4KIIIβ led to markedly higher association of clathrin (∼3.5‐fold), the adaptor AP‐2 (∼2.8‐fold) and the plus‐end directed motor Kif13b (>5‐fold) to these vesicles and to higher content of lysosomal constituents (LAMP1 and Cath D shown here) (Figure 6F). The increase in AP‐2 was in clear contrast to the pronounced (∼3‐fold) decrease in AP‐3. Although more discrete, we also observed enhanced association of Rab7, Rab9 and Rab11 in the PI4KIIIβ‐defective carriers (Figure 6F)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:VeKvrfk0rSWIQfepmlhaOm293MI10090 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 intact-crc:EB3F9D66BC246A54|rigid:nIgmXqpW0Tg1sjQu7pAQl7gZ7ns false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P22892 uniprotkb:Q9CQD1 intact:EBI-1040262|ensembl:ENSMUSP00000034171 intact:EBI-647712|uniprotkb:Q9DCN5|uniprotkb:Q3UCX7|uniprotkb:Q4VAA1|ensembl:ENSMUSP00000017975 psi-mi:ap1g1_mouse(display_long)|uniprotkb:Ap1g1(gene name)|psi-mi:Ap1g1(display_short)|uniprotkb:Adtg(gene name synonym)|uniprotkb:Clapg1(gene name synonym)|uniprotkb:Adaptor protein complex AP-1 subunit gamma-1(gene name synonym)|uniprotkb:Golgi adaptor HA1/AP1 adaptin subunit gamma-1(gene name synonym)|uniprotkb:Gamma1-adaptin(gene name synonym)|uniprotkb:Gamma-adaptin(gene name synonym)|uniprotkb:Clathrin assembly protein complex 1 gamma-1 large chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 1 subunit gamma-1(gene name synonym) psi-mi:rab5a_mouse(display_long)|uniprotkb:Rab5a(gene name)|psi-mi:Rab5a(display_short)|uniprotkb:nnyRab5a(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103779|imex:IM-22093-11 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001288140.1|ensembl:ENSMUSG00000031731(gene)|ensembl:ENSMUST00000034171(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0005518"(collagen binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019894"(kinesin binding)|go:"GO:0030121"(AP-1 adaptor complex)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030742"(GTP-dependent protein binding)|go:"GO:0031267"(small GTPase binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035646"(endosome to melanosome transport)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055037"(recycling endosome)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0140312"(cargo adaptor activity)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR008152(Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain)|interpro:IPR008153(Clathrin adaptor, gamma-adaptin, appendage)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR013041(Clathrin/coatomer adaptor, adaptin-like, appendage, Ig-like subdomain)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017107(Adaptor protein complex AP-1, gamma subunit)|mint:P22892|rcsb pdb:1GYU|rcsb pdb:1GYV|rcsb pdb:1GYW|rcsb pdb:1W63|rcsb pdb:2A7B|rcsb pdb:3ZY7|rcsb pdb:4HMY|rcsb pdb:4P6Z|rcsb pdb:6CM9|rcsb pdb:6CRI|rcsb pdb:6D83|rcsb pdb:6D84|rcsb pdb:6DFF|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|go:"GO:0015630"(microtubule cytoskeleton) refseq:NP_080163.1|ensembl:ENSMUSG00000017831(gene)|ensembl:ENSMUST00000017975(transcript)|go:"GO:0001726"(ruffle)|go:"GO:0003924"(GTPase activity)|go:"GO:0003925"(G protein activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005768"(endosome)|go:"GO:0005769"(early endosome)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0006909"(phagocytosis)|go:"GO:0007032"(endosome organization)|go:"GO:0010008"(endosome membrane)|go:"GO:0012505"(endomembrane system)|go:"GO:0014911"(positive regulation of smooth muscle cell migration)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0019001"(guanyl nucleotide binding)|go:"GO:0019003"(GDP binding)|go:"GO:0030100"(regulation of endocytosis)|go:"GO:0030139"(endocytic vesicle)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030670"(phagocytic vesicle membrane)|go:"GO:0032009"(early phagosome)|go:"GO:0032991"(protein-containing complex)|go:"GO:0036465"(synaptic vesicle recycling)|go:"GO:0036477"(somatodendritic compartment)|go:"GO:0039694"(viral RNA genome replication)|go:"GO:0042470"(melanosome)|go:"GO:0042589"(zymogen granule membrane)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043679"(axon terminus)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045121"(membrane raft)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0045921"(positive regulation of exocytosis)|go:"GO:0048169"(regulation of long-term neuronal synaptic plasticity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048661"(positive regulation of smooth muscle cell proliferation)|go:"GO:0051021"(GDP-dissociation inhibitor binding)|go:"GO:0051036"(regulation of endosome size)|go:"GO:0051489"(regulation of filopodium assembly)|go:"GO:0055037"(recycling endosome)|go:"GO:0098559"(cytoplasmic side of early endosome membrane)|go:"GO:0098842"(postsynaptic early endosome)|go:"GO:0098993"(anchored component of synaptic vesicle membrane)|go:"GO:0150093"(amyloid-beta clearance by transcytosis)|go:"GO:2000286"(receptor internalization involved in canonical Wnt signaling pathway)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR027417|reactome:R-MMU-1660499|reactome:R-MMU-8856828|reactome:R-MMU-8873719|reactome:R-MMU-8876198|reactome:R-MMU-983231|go:"GO:0008021"(synaptic vesicle) go:"GO:0005829"(cytosol) - - figure legend:f6f|comment:"Comparison of the association of each of these components with carriers isolated from control and PI4KIIIβ(−) cells (Figure 6F) revealed that loss of PI4KIIIβ led to markedly higher association of clathrin (∼3.5‐fold), the adaptor AP‐2 (∼2.8‐fold) and the plus‐end directed motor Kif13b (>5‐fold) to these vesicles and to higher content of lysosomal constituents (LAMP1 and Cath D shown here) (Figure 6F). The increase in AP‐2 was in clear contrast to the pronounced (∼3‐fold) decrease in AP‐3. Although more discrete, we also observed enhanced association of Rab7, Rab9 and Rab11 in the PI4KIIIβ‐defective carriers (Figure 6F)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:VeKvrfk0rSWIQfepmlhaOm293MI10090 rogid:TleQFpdeUdtlHFp7sMsgWpx6p8E10090 intact-crc:EB3F9D66BC246A54|rigid:nIgmXqpW0Tg1sjQu7pAQl7gZ7ns false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P18242 uniprotkb:P17047 intact:EBI-738562|ensembl:ENSMUSP00000121203 intact:EBI-1564749|uniprotkb:A2A430|uniprotkb:Q3TXG5|uniprotkb:Q8BSG8|ensembl:ENSMUSP00000016678 psi-mi:catd_mouse(display_long)|uniprotkb:Ctsd(gene name)|psi-mi:Ctsd(display_short) psi-mi:lamp2_mouse(display_long)|uniprotkb:Lysosomal membrane glycoprotein type B(gene name synonym)|uniprotkb:CD107 antigen-like family member B(gene name synonym)|uniprotkb:Lamp2(gene name)|psi-mi:Lamp2(display_short)|uniprotkb:Lamp-2(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103790|imex:IM-22093-12 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_034113.1|go:"GO:0042159"(lipoprotein catabolic process)|go:"GO:0042277"(peptide binding)|go:"GO:0042470"(melanosome)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0045121"(membrane raft)|go:"GO:0062023"(collagen-containing extracellular matrix)|go:"GO:0070001"(aspartic-type peptidase activity)|go:"GO:0070201"(regulation of establishment of protein localization)|interpro:IPR001461(Peptidase A1)|interpro:IPR001969(Peptidase aspartic, active site)|interpro:IPR012848(Propeptide, peptidase A1)|go:"GO:0004190"(aspartic-type endopeptidase activity)|interpro:IPR021109|interpro:IPR033121|interpro:IPR033144|mint:P18242|go:"GO:0005615"(extracellular space)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|reactome:R-MMU-1442490|go:"GO:0006508"(proteolysis)|go:"GO:0008233"(peptidase activity)|reactome:R-MMU-2022377|reactome:R-MMU-2132295|reactome:R-MMU-6798695|go:"GO:0010008"(endosome membrane)|go:"GO:0016787"(hydrolase activity)|ensembl:ENSMUSG00000007891(gene)|ensembl:ENSMUST00000151120(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0004175"(endopeptidase activity) refseq:NP_001017959.1|refseq:NP_034815.2|ensembl:ENSMUSG00000016534(gene)|ensembl:ENSMUST00000016678(transcript)|go:"GO:0005615"(extracellular space)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005768"(endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0006605"(protein targeting)|go:"GO:0006914"(autophagy)|go:"GO:0009267"(cellular response to starvation)|go:"GO:0016020"(membrane)|go:"GO:0019899"(enzyme binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030670"(phagocytic vesicle membrane)|go:"GO:0031088"(platelet dense granule membrane)|go:"GO:0031333"(negative regulation of protein-containing complex assembly)|go:"GO:0031647"(regulation of protein stability)|go:"GO:0031902"(late endosome membrane)|go:"GO:0044754"(autolysosome)|go:"GO:0046716"(muscle cell cellular homeostasis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050821"(protein stabilization)|go:"GO:0061684"(chaperone-mediated autophagy)|go:"GO:0061740"(protein targeting to lysosome involved in chaperone-mediated autophagy)|go:"GO:0061742"(chaperone-mediated autophagy translocation complex)|go:"GO:0070062"(extracellular exosome)|go:"GO:0072594"(establishment of protein localization to organelle)|go:"GO:0097352"(autophagosome maturation)|go:"GO:0097637"(integral component of autophagosome membrane)|go:"GO:1905146"(lysosomal protein catabolic process)|go:"GO:1990836"(lysosomal matrix)|interpro:IPR002000("Lysosome-associated membrane glycoprotein (Lamp)/CD68")|interpro:IPR018134|mint:P17047|rcsb pdb:5GV3|reactome:R-MMU-114608|reactome:R-MMU-6798695 go:"GO:0005829"(cytosol) - - figure legend:sf6a|comment:"Comparison of the association of each of these components with carriers isolated from control and PI4KIIIβ(−) cells (Figure 6F) revealed that loss of PI4KIIIβ led to markedly higher association of clathrin (∼3.5‐fold), the adaptor AP‐2 (∼2.8‐fold) and the plus‐end directed motor Kif13b (>5‐fold) to these vesicles and to higher content of lysosomal constituents (LAMP1 and Cath D shown here) (Figure 6F). The increase in AP‐2 was in clear contrast to the pronounced (∼3‐fold) decrease in AP‐3. Although more discrete, we also observed enhanced association of Rab7, Rab9 and Rab11 in the PI4KIIIβ‐defective carriers (Figure 6F)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:PrpcvWCAFSmTF9ECBCB/zMV6d9M10090 rogid:em1v9qxpoHR13ocQdAffj+zQSDE10090 intact-crc:FB78DDCD8FBFCEC4|rigid:emioaYkvyrTIMNbnGBFkbBLof28 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P18242 uniprotkb:Q2TBE6 intact:EBI-738562|ensembl:ENSMUSP00000121203 intact:EBI-8159115|ensembl:ENSMUSP00000069284|ensembl:ENSMUSP00000158574|intact:MINT-1865952|intact:MINT-8427650 psi-mi:catd_mouse(display_long)|uniprotkb:Ctsd(gene name)|psi-mi:Ctsd(display_short) psi-mi:p4k2a_mouse(display_long)|uniprotkb:Pi4k2a(gene name)|psi-mi:Pi4k2a(display_short)|uniprotkb:Phosphatidylinositol 4-kinase type II-alpha(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103790|imex:IM-22093-12 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_034113.1|go:"GO:0042159"(lipoprotein catabolic process)|go:"GO:0042277"(peptide binding)|go:"GO:0042470"(melanosome)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0045121"(membrane raft)|go:"GO:0062023"(collagen-containing extracellular matrix)|go:"GO:0070001"(aspartic-type peptidase activity)|go:"GO:0070201"(regulation of establishment of protein localization)|interpro:IPR001461(Peptidase A1)|interpro:IPR001969(Peptidase aspartic, active site)|interpro:IPR012848(Propeptide, peptidase A1)|go:"GO:0004190"(aspartic-type endopeptidase activity)|interpro:IPR021109|interpro:IPR033121|interpro:IPR033144|mint:P18242|go:"GO:0005615"(extracellular space)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|reactome:R-MMU-1442490|go:"GO:0006508"(proteolysis)|go:"GO:0008233"(peptidase activity)|reactome:R-MMU-2022377|reactome:R-MMU-2132295|reactome:R-MMU-6798695|go:"GO:0010008"(endosome membrane)|go:"GO:0016787"(hydrolase activity)|ensembl:ENSMUSG00000007891(gene)|ensembl:ENSMUST00000151120(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0004175"(endopeptidase activity) refseq:XP_011245707.1|ensembl:ENSMUSG00000025178(gene)|ensembl:ENSMUST00000066778(transcript)|ensembl:ENSMUST00000235932(transcript)|go:"GO:0002561"(basophil degranulation)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006661"(phosphatidylinositol biosynthetic process)|go:"GO:0007030"(Golgi organization)|go:"GO:0007032"(endosome organization)|go:"GO:0030425"(dendrite)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031083"(BLOC-1 complex)|go:"GO:0031224"(intrinsic component of membrane)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031901"(early endosome membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0035651"(AP-3 adaptor complex binding)|go:"GO:0035838"(growing cell tip)|go:"GO:0042734"(presynaptic membrane)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043204"(perikaryon)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045121"(membrane raft)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0070382"(exocytic vesicle)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR039756|mint:Q2TBE6|reactome:R-MMU-1660499|reactome:R-MMU-1660514|reactome:R-MMU-1660516|refseq:NP_663476.1|uniprotkb:Q8VC15(q8vc15_mouse) go:"GO:0005829"(cytosol) - comment:mint figure legend:sf6a|comment:"Comparison of the association of each of these components with carriers isolated from control and PI4KIIIβ(−) cells (Figure 6F) revealed that loss of PI4KIIIβ led to markedly higher association of clathrin (∼3.5‐fold), the adaptor AP‐2 (∼2.8‐fold) and the plus‐end directed motor Kif13b (>5‐fold) to these vesicles and to higher content of lysosomal constituents (LAMP1 and Cath D shown here) (Figure 6F). The increase in AP‐2 was in clear contrast to the pronounced (∼3‐fold) decrease in AP‐3. Although more discrete, we also observed enhanced association of Rab7, Rab9 and Rab11 in the PI4KIIIβ‐defective carriers (Figure 6F)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:PrpcvWCAFSmTF9ECBCB/zMV6d9M10090 rogid:215wPS5InduQ3iDmYxvPJySrxC810090 intact-crc:FB78DDCD8FBFCEC4|rigid:emioaYkvyrTIMNbnGBFkbBLof28 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P18242 uniprotkb:Q8BKC8 intact:EBI-738562|ensembl:ENSMUSP00000121203 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 psi-mi:catd_mouse(display_long)|uniprotkb:Ctsd(gene name)|psi-mi:Ctsd(display_short) psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103790|imex:IM-22093-12 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_034113.1|go:"GO:0042159"(lipoprotein catabolic process)|go:"GO:0042277"(peptide binding)|go:"GO:0042470"(melanosome)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0045121"(membrane raft)|go:"GO:0062023"(collagen-containing extracellular matrix)|go:"GO:0070001"(aspartic-type peptidase activity)|go:"GO:0070201"(regulation of establishment of protein localization)|interpro:IPR001461(Peptidase A1)|interpro:IPR001969(Peptidase aspartic, active site)|interpro:IPR012848(Propeptide, peptidase A1)|go:"GO:0004190"(aspartic-type endopeptidase activity)|interpro:IPR021109|interpro:IPR033121|interpro:IPR033144|mint:P18242|go:"GO:0005615"(extracellular space)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|reactome:R-MMU-1442490|go:"GO:0006508"(proteolysis)|go:"GO:0008233"(peptidase activity)|reactome:R-MMU-2022377|reactome:R-MMU-2132295|reactome:R-MMU-6798695|go:"GO:0010008"(endosome membrane)|go:"GO:0016787"(hydrolase activity)|ensembl:ENSMUSG00000007891(gene)|ensembl:ENSMUST00000151120(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0004175"(endopeptidase activity) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 go:"GO:0005829"(cytosol) - - figure legend:sf6a|comment:"Comparison of the association of each of these components with carriers isolated from control and PI4KIIIβ(−) cells (Figure 6F) revealed that loss of PI4KIIIβ led to markedly higher association of clathrin (∼3.5‐fold), the adaptor AP‐2 (∼2.8‐fold) and the plus‐end directed motor Kif13b (>5‐fold) to these vesicles and to higher content of lysosomal constituents (LAMP1 and Cath D shown here) (Figure 6F). The increase in AP‐2 was in clear contrast to the pronounced (∼3‐fold) decrease in AP‐3. Although more discrete, we also observed enhanced association of Rab7, Rab9 and Rab11 in the PI4KIIIβ‐defective carriers (Figure 6F)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:PrpcvWCAFSmTF9ECBCB/zMV6d9M10090 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 intact-crc:FB78DDCD8FBFCEC4|rigid:emioaYkvyrTIMNbnGBFkbBLof28 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P22892 uniprotkb:O54774 intact:EBI-1040262|ensembl:ENSMUSP00000034171 intact:EBI-775247|ensembl:ENSMUSP00000020420 psi-mi:ap1g1_mouse(display_long)|uniprotkb:Ap1g1(gene name)|psi-mi:Ap1g1(display_short)|uniprotkb:Adtg(gene name synonym)|uniprotkb:Clapg1(gene name synonym)|uniprotkb:Adaptor protein complex AP-1 subunit gamma-1(gene name synonym)|uniprotkb:Golgi adaptor HA1/AP1 adaptin subunit gamma-1(gene name synonym)|uniprotkb:Gamma1-adaptin(gene name synonym)|uniprotkb:Gamma-adaptin(gene name synonym)|uniprotkb:Clathrin assembly protein complex 1 gamma-1 large chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 1 subunit gamma-1(gene name synonym) psi-mi:ap3d1_mouse(display_long)|uniprotkb:AP-3 complex subunit delta(gene name synonym)|uniprotkb:Delta-adaptin(gene name synonym)|uniprotkb:Ap3d1(gene name)|psi-mi:Ap3d1(display_short)|uniprotkb:Ap3d(gene name synonym)|uniprotkb:Adaptor-related protein complex 3 subunit delta-1(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103742|imex:IM-22093-10 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001288140.1|ensembl:ENSMUSG00000031731(gene)|ensembl:ENSMUST00000034171(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0005518"(collagen binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019894"(kinesin binding)|go:"GO:0030121"(AP-1 adaptor complex)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030742"(GTP-dependent protein binding)|go:"GO:0031267"(small GTPase binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035646"(endosome to melanosome transport)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055037"(recycling endosome)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0140312"(cargo adaptor activity)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR008152(Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain)|interpro:IPR008153(Clathrin adaptor, gamma-adaptin, appendage)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR013041(Clathrin/coatomer adaptor, adaptin-like, appendage, Ig-like subdomain)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017107(Adaptor protein complex AP-1, gamma subunit)|mint:P22892|rcsb pdb:1GYU|rcsb pdb:1GYV|rcsb pdb:1GYW|rcsb pdb:1W63|rcsb pdb:2A7B|rcsb pdb:3ZY7|rcsb pdb:4HMY|rcsb pdb:4P6Z|rcsb pdb:6CM9|rcsb pdb:6CRI|rcsb pdb:6D83|rcsb pdb:6D84|rcsb pdb:6DFF|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|go:"GO:0015630"(microtubule cytoskeleton) refseq:NP_031486.1|dip:DIP-32163N|ensembl:ENSMUSG00000020198(gene)|ensembl:ENSMUST00000020420(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006829"(zinc ion transport)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0008089"(anterograde axonal transport)|go:"GO:0010008"(endosome membrane)|go:"GO:0016182"(synaptic vesicle budding from endosome)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0030123"(AP-3 adaptor complex)|go:"GO:0030424"(axon)|go:"GO:0033365"(protein localization to organelle)|go:"GO:0035646"(endosome to melanosome transport)|go:"GO:0043195"(terminal bouton)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0048007"(antigen processing and presentation, exogenous lipid antigen via MHC class Ib)|go:"GO:0048490"(anterograde synaptic vesicle transport)|go:"GO:0048499"(synaptic vesicle membrane organization)|go:"GO:0051138"(positive regulation of NK T cell differentiation)|go:"GO:0061088"(regulation of sequestering of zinc ion)|go:"GO:0072657"(protein localization to membrane)|go:"GO:0098793"(presynapse)|go:"GO:0098794"(postsynapse)|go:"GO:0098830"(presynaptic endosome)|go:"GO:0098943"(neurotransmitter receptor transport, postsynaptic endosome to lysosome)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099003"(vesicle-mediated transport in synapse)|go:"GO:1904115"(axon cytoplasm)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR010474(Bovine leukaemia virus receptor)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017105(Adaptor protein complex AP-3, delta subunit)|mint:O54774 go:"GO:0005829"(cytosol) - - figure legend:f6c|comment:"We next analysed the distribution of PI4KIIIβ, lysosomal proteins and trafficking molecules into sorting vesicles isolated from the cytosolic fraction by differential centrifugation of increasing force (300 000–500 000 g). PI4KIIIβ, clathrin and lysosomal membrane proteins (LAMP1 shown here) demonstrated a gradual enrichment towards the smaller sized vesicles (Figure 6C)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:VeKvrfk0rSWIQfepmlhaOm293MI10090 rogid:q83u6ORB4zYNuQihE4PI1rhvSkY10090 intact-crc:4A6C60E59D8E6145|rigid:P90MBx3FmaOA9WQuhKnxlfXJYQ4 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P22892 uniprotkb:Q68FD5 intact:EBI-1040262|ensembl:ENSMUSP00000034171 intact:EBI-769168|ensembl:ENSMUSP00000099475 psi-mi:ap1g1_mouse(display_long)|uniprotkb:Ap1g1(gene name)|psi-mi:Ap1g1(display_short)|uniprotkb:Adtg(gene name synonym)|uniprotkb:Clapg1(gene name synonym)|uniprotkb:Adaptor protein complex AP-1 subunit gamma-1(gene name synonym)|uniprotkb:Golgi adaptor HA1/AP1 adaptin subunit gamma-1(gene name synonym)|uniprotkb:Gamma1-adaptin(gene name synonym)|uniprotkb:Gamma-adaptin(gene name synonym)|uniprotkb:Clathrin assembly protein complex 1 gamma-1 large chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 1 subunit gamma-1(gene name synonym) psi-mi:clh1_mouse(display_long)|uniprotkb:Cltc(gene name)|psi-mi:Cltc(display_short) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103742|imex:IM-22093-10 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001288140.1|ensembl:ENSMUSG00000031731(gene)|ensembl:ENSMUST00000034171(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0005518"(collagen binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019894"(kinesin binding)|go:"GO:0030121"(AP-1 adaptor complex)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030742"(GTP-dependent protein binding)|go:"GO:0031267"(small GTPase binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035646"(endosome to melanosome transport)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055037"(recycling endosome)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0140312"(cargo adaptor activity)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR008152(Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain)|interpro:IPR008153(Clathrin adaptor, gamma-adaptin, appendage)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR013041(Clathrin/coatomer adaptor, adaptin-like, appendage, Ig-like subdomain)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017107(Adaptor protein complex AP-1, gamma subunit)|mint:P22892|rcsb pdb:1GYU|rcsb pdb:1GYV|rcsb pdb:1GYW|rcsb pdb:1W63|rcsb pdb:2A7B|rcsb pdb:3ZY7|rcsb pdb:4HMY|rcsb pdb:4P6Z|rcsb pdb:6CM9|rcsb pdb:6CRI|rcsb pdb:6D83|rcsb pdb:6D84|rcsb pdb:6DFF|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|go:"GO:0015630"(microtubule cytoskeleton) refseq:NP_001003908.1|dip:DIP-31947N|ensembl:ENSMUSG00000047126(gene)|ensembl:ENSMUST00000103186(transcript)|go:"GO:0000278"(mitotic cell cycle)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0005198"(structural molecule activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0005768"(endosome)|go:"GO:0005819"(spindle)|go:"GO:0005829"(cytosol)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006914"(autophagy)|go:"GO:0007030"(Golgi organization)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016020"(membrane)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030117"(membrane coat)|go:"GO:0030118"(clathrin coat)|go:"GO:0030130"(clathrin coat of trans-Golgi network vesicle)|go:"GO:0030132"(clathrin coat of coated pit)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030315"(T-tubule)|go:"GO:0030506"(ankyrin binding)|go:"GO:0031072"(heat shock protein binding)|go:"GO:0031523"(Myb complex)|go:"GO:0031623"(receptor internalization)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033572"(transferrin transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0042277"(peptide binding)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043209"(myelin sheath)|go:"GO:0045334"(clathrin-coated endocytic vesicle)|go:"GO:0048268"(clathrin coat assembly)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0050750"(low-density lipoprotein particle receptor binding)|go:"GO:0051301"(cell division)|go:"GO:0060236"(regulation of mitotic spindle organization)|go:"GO:0071439"(clathrin complex)|go:"GO:0072583"(clathrin-dependent endocytosis)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090307"(mitotic spindle assembly)|go:"GO:0097718"(disordered domain specific binding)|go:"GO:0098684"(photoreceptor ribbon synapse)|go:"GO:0098835"(presynaptic endocytic zone membrane)|go:"GO:0098850"(extrinsic component of synaptic vesicle membrane)|go:"GO:0150093"(amyloid-beta clearance by transcytosis)|go:"GO:1900126"(negative regulation of hyaluronan biosynthetic process)|go:"GO:1903077"(negative regulation of protein localization to plasma membrane)|go:"GO:1990381"(ubiquitin-specific protease binding)|go:"GO:1990498"(mitotic spindle microtubule)|interpro:IPR000547(Clathrin, heavy chain/VPS, 7-fold repeat)|interpro:IPR011990(Tetratricopeptide-like helical)|interpro:IPR015348(Clathrin, heavy chain, linker, core motif)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR016025(Clathrin, heavy chain, linker and propeller)|interpro:IPR016341(Clathrin, heavy chain)|interpro:IPR022365|mint:Q68FD5|rcsb pdb:5ODS|reactome:R-MMU-177504|reactome:R-MMU-190873|reactome:R-MMU-196025|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|reactome:R-MMU-437239|reactome:R-MMU-5099900|reactome:R-MMU-5140745|reactome:R-MMU-8856825|reactome:R-MMU-8856828|reactome:R-MMU-8866427|reactome:R-MMU-8964038|reactome:R-MMU-9013420|reactome:R-MMU-9013424|go:"GO:0032051"(clathrin light chain binding) go:"GO:0005829"(cytosol) - - figure legend:f6c|comment:"We next analysed the distribution of PI4KIIIβ, lysosomal proteins and trafficking molecules into sorting vesicles isolated from the cytosolic fraction by differential centrifugation of increasing force (300 000–500 000 g). PI4KIIIβ, clathrin and lysosomal membrane proteins (LAMP1 shown here) demonstrated a gradual enrichment towards the smaller sized vesicles (Figure 6C)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:VeKvrfk0rSWIQfepmlhaOm293MI10090 rogid:iHg/TOG2rWePqtsEmuc/szn/chI10090 intact-crc:4A6C60E59D8E6145|rigid:P90MBx3FmaOA9WQuhKnxlfXJYQ4 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P22892 uniprotkb:P11438 intact:EBI-1040262|ensembl:ENSMUSP00000034171 intact:EBI-987439|uniprotkb:Q62020|ensembl:ENSMUSP00000033824 psi-mi:ap1g1_mouse(display_long)|uniprotkb:Ap1g1(gene name)|psi-mi:Ap1g1(display_short)|uniprotkb:Adtg(gene name synonym)|uniprotkb:Clapg1(gene name synonym)|uniprotkb:Adaptor protein complex AP-1 subunit gamma-1(gene name synonym)|uniprotkb:Golgi adaptor HA1/AP1 adaptin subunit gamma-1(gene name synonym)|uniprotkb:Gamma1-adaptin(gene name synonym)|uniprotkb:Gamma-adaptin(gene name synonym)|uniprotkb:Clathrin assembly protein complex 1 gamma-1 large chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 1 subunit gamma-1(gene name synonym) psi-mi:lamp1_mouse(display_long)|uniprotkb:Lysosomal membrane glycoprotein A(gene name synonym)|uniprotkb:120 kDa lysosomal membrane glycoprotein(gene name synonym)|uniprotkb:LGP-120(gene name synonym)|uniprotkb:P2B(gene name synonym)|uniprotkb:CD107 antigen-like family member A(gene name synonym)|uniprotkb:Lamp1(gene name)|psi-mi:Lamp1(display_short)|uniprotkb:Lamp-1(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103742|imex:IM-22093-10 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001288140.1|ensembl:ENSMUSG00000031731(gene)|ensembl:ENSMUST00000034171(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0005518"(collagen binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019894"(kinesin binding)|go:"GO:0030121"(AP-1 adaptor complex)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030742"(GTP-dependent protein binding)|go:"GO:0031267"(small GTPase binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035646"(endosome to melanosome transport)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055037"(recycling endosome)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0140312"(cargo adaptor activity)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR008152(Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain)|interpro:IPR008153(Clathrin adaptor, gamma-adaptin, appendage)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR013041(Clathrin/coatomer adaptor, adaptin-like, appendage, Ig-like subdomain)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017107(Adaptor protein complex AP-1, gamma subunit)|mint:P22892|rcsb pdb:1GYU|rcsb pdb:1GYV|rcsb pdb:1GYW|rcsb pdb:1W63|rcsb pdb:2A7B|rcsb pdb:3ZY7|rcsb pdb:4HMY|rcsb pdb:4P6Z|rcsb pdb:6CM9|rcsb pdb:6CRI|rcsb pdb:6D83|rcsb pdb:6D84|rcsb pdb:6DFF|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|go:"GO:0015630"(microtubule cytoskeleton) ensembl:ENSMUSG00000031447(gene)|ensembl:ENSMUST00000033824(transcript)|go:"GO:0000421"(autophagosome membrane)|go:"GO:0005737"(cytoplasm)|go:"GO:0005764"(lysosome)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005768"(endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005771"(multivesicular body)|go:"GO:0005773"(vacuole)|refseq:NP_034814.2|go:"GO:0005886"(plasma membrane)|go:"GO:0007283"(spermatogenesis)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0010008"(endosome membrane)|go:"GO:0019899"(enzyme binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030425"(dendrite)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031902"(late endosome membrane)|go:"GO:0031982"(vesicle)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0044194"(cytolytic granule)|go:"GO:0044754"(autolysosome)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048102"(autophagic cell death)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050821"(protein stabilization)|go:"GO:0061474"(phagolysosome membrane)|go:"GO:0072594"(establishment of protein localization to organelle)|go:"GO:0005829"(cytosol)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0097208"(alveolar lamellar body)|go:"GO:0101004"(cytolytic granule membrane)|go:"GO:0140507"(granzyme-mediated programmed cell death signaling pathway)|go:"GO:1902513"(regulation of organelle transport along microtubule)|interpro:IPR002000("Lysosome-associated membrane glycoprotein (Lamp)/CD68")|interpro:IPR018134|mint:P11438|rcsb pdb:5GV0|reactome:R-MMU-6798695|refseq:NP_001304282.1 go:"GO:0005829"(cytosol) - - figure legend:f6c|comment:"We next analysed the distribution of PI4KIIIβ, lysosomal proteins and trafficking molecules into sorting vesicles isolated from the cytosolic fraction by differential centrifugation of increasing force (300 000–500 000 g). PI4KIIIβ, clathrin and lysosomal membrane proteins (LAMP1 shown here) demonstrated a gradual enrichment towards the smaller sized vesicles (Figure 6C)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:VeKvrfk0rSWIQfepmlhaOm293MI10090 rogid:34kBKJCpWncY9gLqL2lEx3Yn01I10090 intact-crc:4A6C60E59D8E6145|rigid:P90MBx3FmaOA9WQuhKnxlfXJYQ4 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P22892 uniprotkb:Q8BKC8 intact:EBI-1040262|ensembl:ENSMUSP00000034171 intact:EBI-9103749|uniprotkb:Q14CH6|uniprotkb:Q14DJ4|uniprotkb:Q3TA58|uniprotkb:Q68FH2|uniprotkb:Q8C146|ensembl:ENSMUSP00000102872 psi-mi:ap1g1_mouse(display_long)|uniprotkb:Ap1g1(gene name)|psi-mi:Ap1g1(display_short)|uniprotkb:Adtg(gene name synonym)|uniprotkb:Clapg1(gene name synonym)|uniprotkb:Adaptor protein complex AP-1 subunit gamma-1(gene name synonym)|uniprotkb:Golgi adaptor HA1/AP1 adaptin subunit gamma-1(gene name synonym)|uniprotkb:Gamma1-adaptin(gene name synonym)|uniprotkb:Gamma-adaptin(gene name synonym)|uniprotkb:Clathrin assembly protein complex 1 gamma-1 large chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 1 subunit gamma-1(gene name synonym) psi-mi:pi4kb_mouse(display_long)|uniprotkb:Pi4kb(gene name)|psi-mi:Pi4kb(display_short)|uniprotkb:Pik4cb(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103742|imex:IM-22093-10 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001288140.1|ensembl:ENSMUSG00000031731(gene)|ensembl:ENSMUST00000034171(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0005518"(collagen binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019894"(kinesin binding)|go:"GO:0030121"(AP-1 adaptor complex)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030742"(GTP-dependent protein binding)|go:"GO:0031267"(small GTPase binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035646"(endosome to melanosome transport)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055037"(recycling endosome)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0140312"(cargo adaptor activity)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR008152(Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain)|interpro:IPR008153(Clathrin adaptor, gamma-adaptin, appendage)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR013041(Clathrin/coatomer adaptor, adaptin-like, appendage, Ig-like subdomain)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017107(Adaptor protein complex AP-1, gamma subunit)|mint:P22892|rcsb pdb:1GYU|rcsb pdb:1GYV|rcsb pdb:1GYW|rcsb pdb:1W63|rcsb pdb:2A7B|rcsb pdb:3ZY7|rcsb pdb:4HMY|rcsb pdb:4P6Z|rcsb pdb:6CM9|rcsb pdb:6CRI|rcsb pdb:6D83|rcsb pdb:6D84|rcsb pdb:6DFF|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|go:"GO:0015630"(microtubule cytoskeleton) refseq:NP_001280644.1|refseq:NP_001280645.1|refseq:NP_780565.2|ensembl:ENSMUSG00000038861(gene)|ensembl:ENSMUST00000107251(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0004430"(1-phosphatidylinositol 4-kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0016020"(membrane)|go:"GO:0030867"(rough endoplasmic reticulum membrane)|go:"GO:0046854"(phosphatidylinositol phosphate biosynthetic process)|go:"GO:0048015"(phosphatidylinositol-mediated signaling)|go:"GO:0052742"(phosphatidylinositol kinase activity)|go:"GO:0071889"(14-3-3 protein binding)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR001263(Phosphoinositide 3-kinase accessory region PIK)|interpro:IPR011009(Protein kinase-like)|interpro:IPR015433(Phosphatidylinositol Kinase)|interpro:IPR018936|interpro:IPR036940|reactome:R-MMU-1660514 go:"GO:0005829"(cytosol) - - figure legend:f6c|comment:"We next analysed the distribution of PI4KIIIβ, lysosomal proteins and trafficking molecules into sorting vesicles isolated from the cytosolic fraction by differential centrifugation of increasing force (300 000–500 000 g). PI4KIIIβ, clathrin and lysosomal membrane proteins (LAMP1 shown here) demonstrated a gradual enrichment towards the smaller sized vesicles (Figure 6C)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:VeKvrfk0rSWIQfepmlhaOm293MI10090 rogid:EthNtdLoDiTOujh1YUqOXDDb2B410090 intact-crc:4A6C60E59D8E6145|rigid:P90MBx3FmaOA9WQuhKnxlfXJYQ4 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P22892 uniprotkb:Q68FD5 intact:EBI-1040262|ensembl:ENSMUSP00000034171 intact:EBI-769168|ensembl:ENSMUSP00000099475 psi-mi:ap1g1_mouse(display_long)|uniprotkb:Ap1g1(gene name)|psi-mi:Ap1g1(display_short)|uniprotkb:Adtg(gene name synonym)|uniprotkb:Clapg1(gene name synonym)|uniprotkb:Adaptor protein complex AP-1 subunit gamma-1(gene name synonym)|uniprotkb:Golgi adaptor HA1/AP1 adaptin subunit gamma-1(gene name synonym)|uniprotkb:Gamma1-adaptin(gene name synonym)|uniprotkb:Gamma-adaptin(gene name synonym)|uniprotkb:Clathrin assembly protein complex 1 gamma-1 large chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 1 subunit gamma-1(gene name synonym) psi-mi:clh1_mouse(display_long)|uniprotkb:Cltc(gene name)|psi-mi:Cltc(display_short) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103735|imex:IM-22093-9 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001288140.1|ensembl:ENSMUSG00000031731(gene)|ensembl:ENSMUST00000034171(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0005518"(collagen binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019894"(kinesin binding)|go:"GO:0030121"(AP-1 adaptor complex)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030742"(GTP-dependent protein binding)|go:"GO:0031267"(small GTPase binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035646"(endosome to melanosome transport)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055037"(recycling endosome)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0140312"(cargo adaptor activity)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR008152(Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain)|interpro:IPR008153(Clathrin adaptor, gamma-adaptin, appendage)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR013041(Clathrin/coatomer adaptor, adaptin-like, appendage, Ig-like subdomain)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017107(Adaptor protein complex AP-1, gamma subunit)|mint:P22892|rcsb pdb:1GYU|rcsb pdb:1GYV|rcsb pdb:1GYW|rcsb pdb:1W63|rcsb pdb:2A7B|rcsb pdb:3ZY7|rcsb pdb:4HMY|rcsb pdb:4P6Z|rcsb pdb:6CM9|rcsb pdb:6CRI|rcsb pdb:6D83|rcsb pdb:6D84|rcsb pdb:6DFF|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|go:"GO:0015630"(microtubule cytoskeleton) refseq:NP_001003908.1|dip:DIP-31947N|ensembl:ENSMUSG00000047126(gene)|ensembl:ENSMUST00000103186(transcript)|go:"GO:0000278"(mitotic cell cycle)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0005198"(structural molecule activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005764"(lysosome)|go:"GO:0005768"(endosome)|go:"GO:0005819"(spindle)|go:"GO:0005829"(cytosol)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006914"(autophagy)|go:"GO:0007030"(Golgi organization)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016020"(membrane)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030117"(membrane coat)|go:"GO:0030118"(clathrin coat)|go:"GO:0030130"(clathrin coat of trans-Golgi network vesicle)|go:"GO:0030132"(clathrin coat of coated pit)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030315"(T-tubule)|go:"GO:0030506"(ankyrin binding)|go:"GO:0031072"(heat shock protein binding)|go:"GO:0031523"(Myb complex)|go:"GO:0031623"(receptor internalization)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033572"(transferrin transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0042277"(peptide binding)|go:"GO:0042383"(sarcolemma)|go:"GO:0042470"(melanosome)|go:"GO:0043209"(myelin sheath)|go:"GO:0045334"(clathrin-coated endocytic vesicle)|go:"GO:0048268"(clathrin coat assembly)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0050750"(low-density lipoprotein particle receptor binding)|go:"GO:0051301"(cell division)|go:"GO:0060236"(regulation of mitotic spindle organization)|go:"GO:0071439"(clathrin complex)|go:"GO:0072583"(clathrin-dependent endocytosis)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090307"(mitotic spindle assembly)|go:"GO:0097718"(disordered domain specific binding)|go:"GO:0098684"(photoreceptor ribbon synapse)|go:"GO:0098835"(presynaptic endocytic zone membrane)|go:"GO:0098850"(extrinsic component of synaptic vesicle membrane)|go:"GO:0150093"(amyloid-beta clearance by transcytosis)|go:"GO:1900126"(negative regulation of hyaluronan biosynthetic process)|go:"GO:1903077"(negative regulation of protein localization to plasma membrane)|go:"GO:1990381"(ubiquitin-specific protease binding)|go:"GO:1990498"(mitotic spindle microtubule)|interpro:IPR000547(Clathrin, heavy chain/VPS, 7-fold repeat)|interpro:IPR011990(Tetratricopeptide-like helical)|interpro:IPR015348(Clathrin, heavy chain, linker, core motif)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR016025(Clathrin, heavy chain, linker and propeller)|interpro:IPR016341(Clathrin, heavy chain)|interpro:IPR022365|mint:Q68FD5|rcsb pdb:5ODS|reactome:R-MMU-177504|reactome:R-MMU-190873|reactome:R-MMU-196025|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|reactome:R-MMU-437239|reactome:R-MMU-5099900|reactome:R-MMU-5140745|reactome:R-MMU-8856825|reactome:R-MMU-8856828|reactome:R-MMU-8866427|reactome:R-MMU-8964038|reactome:R-MMU-9013420|reactome:R-MMU-9013424|go:"GO:0032051"(clathrin light chain binding) go:"GO:0005764"(lysosome) - - figure legend:f6a|comment:"Consequently, we first examined possible changes in the lysosomal association of clathrin and its cognate adaptors in PI4KIIIβ‐deficient cells and found that levels of clathrin (Clath HC) were markedly increased (∼3‐fold) in Lys isolated from PI4KIIIβ(−) cells (Figure 6A)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:VeKvrfk0rSWIQfepmlhaOm293MI10090 rogid:iHg/TOG2rWePqtsEmuc/szn/chI10090 intact-crc:250AAFA4A61FAB14|rigid:XR6xztY0J7Ot7IPDNM/gK+vJUZ8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P22892 uniprotkb:O88384 intact:EBI-1040262|ensembl:ENSMUSP00000034171 intact:EBI-775853 psi-mi:ap1g1_mouse(display_long)|uniprotkb:Ap1g1(gene name)|psi-mi:Ap1g1(display_short)|uniprotkb:Adtg(gene name synonym)|uniprotkb:Clapg1(gene name synonym)|uniprotkb:Adaptor protein complex AP-1 subunit gamma-1(gene name synonym)|uniprotkb:Golgi adaptor HA1/AP1 adaptin subunit gamma-1(gene name synonym)|uniprotkb:Gamma1-adaptin(gene name synonym)|uniprotkb:Gamma-adaptin(gene name synonym)|uniprotkb:Clathrin assembly protein complex 1 gamma-1 large chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 1 subunit gamma-1(gene name synonym) psi-mi:vti1b_mouse(display_long)|uniprotkb:Vesicle transport v-SNARE protein Vti1-like 1(gene name synonym)|uniprotkb:Vti1-rp1(gene name synonym)|uniprotkb:Vti1b(gene name)|psi-mi:Vti1b(display_short)|uniprotkb:Vti1l1(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Sridhar et al. (2013) imex:IM-22093|pubmed:23258225 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0471"(MINT) intact:EBI-9103735|imex:IM-22093-9 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001288140.1|ensembl:ENSMUSG00000031731(gene)|ensembl:ENSMUST00000034171(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0005518"(collagen binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019894"(kinesin binding)|go:"GO:0030121"(AP-1 adaptor complex)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030742"(GTP-dependent protein binding)|go:"GO:0031267"(small GTPase binding)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0035646"(endosome to melanosome transport)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043323"(positive regulation of natural killer cell degranulation)|go:"GO:0045954"(positive regulation of natural killer cell mediated cytotoxicity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055037"(recycling endosome)|go:"GO:0090160"(Golgi to lysosome transport)|go:"GO:0140312"(cargo adaptor activity)|interpro:IPR002553(Clathrin/coatomer adaptor, adaptin-like, N-terminal)|interpro:IPR008152(Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain)|interpro:IPR008153(Clathrin adaptor, gamma-adaptin, appendage)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR013041(Clathrin/coatomer adaptor, adaptin-like, appendage, Ig-like subdomain)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR017107(Adaptor protein complex AP-1, gamma subunit)|mint:P22892|rcsb pdb:1GYU|rcsb pdb:1GYV|rcsb pdb:1GYW|rcsb pdb:1W63|rcsb pdb:2A7B|rcsb pdb:3ZY7|rcsb pdb:4HMY|rcsb pdb:4P6Z|rcsb pdb:6CM9|rcsb pdb:6CRI|rcsb pdb:6D83|rcsb pdb:6D84|rcsb pdb:6DFF|reactome:R-MMU-2132295|reactome:R-MMU-432720|reactome:R-MMU-432722|go:"GO:0015630"(microtubule cytoskeleton) dip:DIP-32212N|reactome:R-MMU-114608|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005765"(lysosomal membrane)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019869"(chloride channel inhibitor activity)|go:"GO:0031201"(SNARE complex)|go:"GO:0031225"(anchored component of membrane)|go:"GO:0031901"(early endosome membrane)|go:"GO:0031902"(late endosome membrane)|go:"GO:0031982"(vesicle)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055037"(recycling endosome)|go:"GO:0055038"(recycling endosome membrane)|go:"GO:1903076"(regulation of protein localization to plasma membrane)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR007705(Vesicle transport v-SNARE)|interpro:IPR010989(t-SNARE)|interpro:IPR038407|rcsb pdb:1GL2|rcsb pdb:2QYW go:"GO:0005764"(lysosome) - - figure legend:f6a|comment:"Consequently, we first examined possible changes in the lysosomal association of clathrin and its cognate adaptors in PI4KIIIβ‐deficient cells and found that levels of clathrin (Clath HC) were markedly increased (∼3‐fold) in Lys isolated from PI4KIIIβ(−) cells (Figure 6A)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/01/15 2014/10/16 rogid:VeKvrfk0rSWIQfepmlhaOm293MI10090 rogid:eFI7zI5irsKyRiau/V4OxefEDIM10090 intact-crc:250AAFA4A61FAB14|rigid:XR6xztY0J7Ot7IPDNM/gK+vJUZ8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot)