#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P02469	uniprotkb:P31696	intact:EBI-6662997|ensembl:ENSMUSP00000002979|ensembl:ENSMUSP00000132778|uniprotkb:E9PXZ9	intact:EBI-457650|uniprotkb:Q90609|uniprotkb:Q90685	psi-mi:lamb1_mouse(display_long)|uniprotkb:Lamb1(gene name)|psi-mi:Lamb1(display_short)|uniprotkb:Lamb-1(gene name synonym)|uniprotkb:Lamb1-1(gene name synonym)|uniprotkb:Laminin B1 chain(gene name synonym)|uniprotkb:Laminin-1 subunit beta(gene name synonym)|uniprotkb:Laminin-10 subunit beta(gene name synonym)|uniprotkb:Laminin-12 subunit beta(gene name synonym)|uniprotkb:Laminin-2 subunit beta(gene name synonym)|uniprotkb:Laminin-6 subunit beta(gene name synonym)|uniprotkb:Laminin-8 subunit beta(gene name synonym)	psi-mi:agrin_chick(display_long)|uniprotkb:AGRN(gene name)|psi-mi:AGRN(display_short)|uniprotkb:AGRIN(gene name synonym)	psi-mi:"MI:0892"(solid phase assay)	Denzer et al. (1997)	pubmed:9151673|imex:IM-25866	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1335"(HPIDb)	intact:EBI-6662992|imex:IM-25866-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_006515055.2|ensembl:ENSMUSG00000002900(gene)|ensembl:ENSMUST00000002979(transcript)|ensembl:ENSMUST00000169088(transcript)|go:"GO:0005178"(integrin binding)|go:"GO:0005201"(extracellular matrix structural constituent)|go:"GO:0005576"(extracellular region)|go:"GO:0005604"(basement membrane)|go:"GO:0005606"(laminin-1 complex)|go:"GO:0005607"(laminin-2 complex)|go:"GO:0005615"(extracellular space)|go:"GO:0005634"(nucleus)|go:"GO:0007162"(negative regulation of cell adhesion)|go:"GO:0007566"(embryo implantation)|go:"GO:0007611"(learning or memory)|go:"GO:0009887"(animal organ morphogenesis)|go:"GO:0009888"(tissue development)|go:"GO:0016477"(cell migration)|go:"GO:0019899"(enzyme binding)|go:"GO:0021812"(neuronal-glial interaction involved in cerebral cortex radial glia guided migration)|go:"GO:0030335"(positive regulation of cell migration)|go:"GO:0031012"(extracellular matrix)|go:"GO:0031175"(neuron projection development)|go:"GO:0034446"(substrate adhesion-dependent cell spreading)|go:"GO:0042476"(odontogenesis)|go:"GO:0043208"(glycosphingolipid binding)|go:"GO:0043256"(laminin complex)|go:"GO:0043257"(laminin-8 complex)|go:"GO:0043259"(laminin-10 complex)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0062023"(collagen-containing extracellular matrix)|reactome:R-MMU-3000157|reactome:R-MMU-381426|reactome:R-MMU-8874081|go:"GO:0070831"(basement membrane assembly)|reactome:R-MMU-8957275|interpro:IPR000742(EGF-like, type 3)|interpro:IPR002049(EGF-like, laminin)|interpro:IPR008211(Laminin, N-terminal)|interpro:IPR013015(Laminin IV)|mint:P02469|rcsb pdb:4AQS|rcsb pdb:5MC9|refseq:XP_006515056.1	interpro:IPR001881(EGF-like calcium-binding)|interpro:IPR003884(Factor I membrane attack complex)|interpro:IPR008993(TIMP-like, OB-fold)|rcsb pdb:1PZ8|rcsb pdb:1PZ9|rcsb pdb:1JB3|rcsb pdb:1JC7|rcsb pdb:1PXU|rcsb pdb:1PZ7|rcsb pdb:1Q56|rcsb pdb:3I70|interpro:IPR004850(Agrin NtA)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR000152(Aspartic acid and asparagine hydroxylation site)|interpro:IPR000742(EGF-like, type 3)|interpro:IPR002049(EGF-like, laminin)|interpro:IPR003645(Follistatin-like, N-terminal)|interpro:IPR001791(Laminin G)|interpro:IPR002350(Proteinase inhibitor I1, Kazal)|interpro:IPR000082(SEA)|go:"GO:0043113"(receptor clustering)|go:"GO:0043236"(laminin binding)|go:"GO:0043237"(laminin-1 binding)|go:"GO:0043395"(heparan sulfate proteoglycan binding)|go:"GO:0043547"(positive regulation of GTPase activity)|go:"GO:0045121"(membrane raft)|go:"GO:0045178"(basal part of cell)|go:"GO:0045202"(synapse)|go:"GO:0045887"(positive regulation of synaptic assembly at neuromuscular junction)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046847"(filopodium assembly)|go:"GO:0050840"(extracellular matrix binding)|go:"GO:0051491"(positive regulation of filopodium assembly)|go:"GO:0071340"(skeletal muscle acetylcholine-gated channel clustering)|go:"GO:0098684"(photoreceptor ribbon synapse)|go:"GO:0098965"(extracellular matrix of synaptic cleft)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0038023"(signaling receptor activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005539"(glycosaminoglycan binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005604"(basement membrane)|go:"GO:0005615"(extracellular space)|go:"GO:0005886"(plasma membrane)|go:"GO:0007158"(neuron cell-cell adhesion)|go:"GO:0007213"(G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007399"(nervous system development)|go:"GO:0007420"(brain development)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0008201"(heparin binding)|go:"GO:0010977"(negative regulation of neuron projection development)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030154"(cell differentiation)|go:"GO:0030297"(transmembrane receptor protein tyrosine kinase activator activity)|go:"GO:0030424"(axon)|go:"GO:0030548"(acetylcholine receptor regulator activity)|go:"GO:0031012"(extracellular matrix)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0031594"(neuromuscular junction)|go:"GO:0033691"(sialic acid binding)|go:"GO:0035374"(chondroitin sulfate binding)|go:"GO:0002162"(dystroglycan binding)|interpro:IPR036058|interpro:IPR036364	go:"GO:0030548"(acetylcholine receptor regulator activity)	comment:Lamini-1 was purified from Engelbreth-holm-Swarm mouse tumor	comment:"Recombinant cAgrin(7) (7 aa insert, fig 1a) purified from transfected HEK 293 cells.  Recombinant cN25(7)Fc (cDNA construct that encodes an agrin fragment comprising the NH2-terminal region, fig 1a) purified from transfected COS-7 cells."	figure legend:3|comment:"Experiment shows also that:  - interaction of both constructs of Agrin (cAgrin(7) and cN25(7)Fc) with Laminin-1 is based on a direct interaction that does not require nidogen.  - the majority of the binding of Agrin to Laminin-1 is mediated by the NH2-terminal end.  - interaction of Agrin to Laminin-1 is strong (fig 4)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/05/02	2014/10/16	rogid:cUuVqY2JMeRrS9sDYudPy7hSIL010090	rogid:b+RsTdBVRG0NoC+sRNwneIPSA1M9031	intact-crc:2ACF37F57C812DEB|rigid:TAqAHNPK7SeYpj3QyQleGeUtmxg	false	-	sufficient binding region:1-196..196|fc-igg tag:?-?|125i radiolabel:?-?	-	-	psi-mi:"MI:1197"(radioimmunoassay)	psi-mi:"MI:1197"(radioimmunoassay)
uniprotkb:P31696	uniprotkb:A4VAR9	intact:EBI-457650|uniprotkb:Q90609|uniprotkb:Q90685	intact:EBI-6663053	psi-mi:agrin_chick(display_long)|uniprotkb:AGRN(gene name)|psi-mi:AGRN(display_short)|uniprotkb:AGRIN(gene name synonym)	psi-mi:a4var9_chick(display_long)|uniprotkb:DAG1(gene name)|psi-mi:DAG1(display_short)|uniprotkb:Beta-dystroglycan(gene name synonym)|uniprotkb:Dystroglycan(gene name synonym)|uniprotkb:Dystrophin-associated glycoprotein 1(gene name synonym)	psi-mi:"MI:0892"(solid phase assay)	Denzer et al. (1997)	pubmed:9151673|imex:IM-25866	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1335"(HPIDb)	intact:EBI-6663042|imex:IM-25866-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR001881(EGF-like calcium-binding)|interpro:IPR003884(Factor I membrane attack complex)|interpro:IPR008993(TIMP-like, OB-fold)|rcsb pdb:1PZ8|rcsb pdb:1PZ9|rcsb pdb:1JB3|rcsb pdb:1JC7|rcsb pdb:1PXU|rcsb pdb:1PZ7|rcsb pdb:1Q56|rcsb pdb:3I70|interpro:IPR004850(Agrin NtA)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR000152(Aspartic acid and asparagine hydroxylation site)|interpro:IPR000742(EGF-like, type 3)|interpro:IPR002049(EGF-like, laminin)|interpro:IPR003645(Follistatin-like, N-terminal)|interpro:IPR001791(Laminin G)|interpro:IPR002350(Proteinase inhibitor I1, Kazal)|interpro:IPR000082(SEA)|go:"GO:0043113"(receptor clustering)|go:"GO:0043236"(laminin binding)|go:"GO:0043237"(laminin-1 binding)|go:"GO:0043395"(heparan sulfate proteoglycan binding)|go:"GO:0043547"(positive regulation of GTPase activity)|go:"GO:0045121"(membrane raft)|go:"GO:0045178"(basal part of cell)|go:"GO:0045202"(synapse)|go:"GO:0045887"(positive regulation of synaptic assembly at neuromuscular junction)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046847"(filopodium assembly)|go:"GO:0050840"(extracellular matrix binding)|go:"GO:0051491"(positive regulation of filopodium assembly)|go:"GO:0071340"(skeletal muscle acetylcholine-gated channel clustering)|go:"GO:0098684"(photoreceptor ribbon synapse)|go:"GO:0098965"(extracellular matrix of synaptic cleft)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0038023"(signaling receptor activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005539"(glycosaminoglycan binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005604"(basement membrane)|go:"GO:0005615"(extracellular space)|go:"GO:0005886"(plasma membrane)|go:"GO:0007158"(neuron cell-cell adhesion)|go:"GO:0007213"(G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007399"(nervous system development)|go:"GO:0007420"(brain development)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0008201"(heparin binding)|go:"GO:0010977"(negative regulation of neuron projection development)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030154"(cell differentiation)|go:"GO:0030297"(transmembrane receptor protein tyrosine kinase activator activity)|go:"GO:0030424"(axon)|go:"GO:0030548"(acetylcholine receptor regulator activity)|go:"GO:0031012"(extracellular matrix)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0031594"(neuromuscular junction)|go:"GO:0033691"(sialic acid binding)|go:"GO:0035374"(chondroitin sulfate binding)|go:"GO:0002162"(dystroglycan binding)|interpro:IPR036058|interpro:IPR036364	interpro:IPR030398|go:"GO:0005509"(calcium ion binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005856"(cytoskeleton)|go:"GO:0005886"(plasma membrane)|go:"GO:0010470"(regulation of gastrulation)|go:"GO:0010717"(regulation of epithelial to mesenchymal transition)|go:"GO:0016010"(dystrophin-associated glycoprotein complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016323"(basolateral plasma membrane)|go:"GO:0016476"(regulation of embryonic cell shape)|go:"GO:0042383"(sarcolemma)|go:"GO:0043395"(heparan sulfate proteoglycan binding)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:1904261"(positive regulation of basement membrane assembly involved in embryonic body morphogenesis)|refseq:NP_001091009.1|interpro:IPR006644(Dystroglycan-type cadherin-like)|interpro:IPR015919(Cadherin-like)|interpro:IPR008465(Dystroglycan)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR027468|interpro:IPR041631	-	comment:"Recombinant cAgrin(7) (7 aa insert, fig 1a) purified from transfected HEK 293 cells."	comment:a-dystroglycan purified from skeletal muscels	figure legend:3|comment:"Interaction of agrin with alpha-dystroglycan does not require the NH2-terminal fragment (cN25(7)Fc)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/05/02	2017/09/02	rogid:b+RsTdBVRG0NoC+sRNwneIPSA1M9031	rogid:BNAH0bJ5nS/DxfeLZJh3vtn7F0E9031	intact-crc:DC00EB5EBB152F0A|rigid:47dxQbuKzm8J34VI9Tfib0Lds8M	false	125i radiolabel:?-?|necessary binding region:199..199-2081	-	-	-	psi-mi:"MI:1197"(radioimmunoassay)	psi-mi:"MI:1197"(radioimmunoassay)
uniprotkb:P31696	uniprotkb:P02469	intact:EBI-457650|uniprotkb:Q90609|uniprotkb:Q90685	intact:EBI-6662997|ensembl:ENSMUSP00000002979|ensembl:ENSMUSP00000132778|uniprotkb:E9PXZ9	psi-mi:agrin_chick(display_long)|uniprotkb:AGRN(gene name)|psi-mi:AGRN(display_short)|uniprotkb:AGRIN(gene name synonym)	psi-mi:lamb1_mouse(display_long)|uniprotkb:Lamb1(gene name)|psi-mi:Lamb1(display_short)|uniprotkb:Lamb-1(gene name synonym)|uniprotkb:Lamb1-1(gene name synonym)|uniprotkb:Laminin B1 chain(gene name synonym)|uniprotkb:Laminin-1 subunit beta(gene name synonym)|uniprotkb:Laminin-10 subunit beta(gene name synonym)|uniprotkb:Laminin-12 subunit beta(gene name synonym)|uniprotkb:Laminin-2 subunit beta(gene name synonym)|uniprotkb:Laminin-6 subunit beta(gene name synonym)|uniprotkb:Laminin-8 subunit beta(gene name synonym)	psi-mi:"MI:0892"(solid phase assay)	Denzer et al. (1997)	pubmed:9151673|imex:IM-25866	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1335"(HPIDb)	intact:EBI-6666522|imex:IM-25866-9	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR001881(EGF-like calcium-binding)|interpro:IPR003884(Factor I membrane attack complex)|interpro:IPR008993(TIMP-like, OB-fold)|rcsb pdb:1PZ8|rcsb pdb:1PZ9|rcsb pdb:1JB3|rcsb pdb:1JC7|rcsb pdb:1PXU|rcsb pdb:1PZ7|rcsb pdb:1Q56|rcsb pdb:3I70|interpro:IPR004850(Agrin NtA)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR000152(Aspartic acid and asparagine hydroxylation site)|interpro:IPR000742(EGF-like, type 3)|interpro:IPR002049(EGF-like, laminin)|interpro:IPR003645(Follistatin-like, N-terminal)|interpro:IPR001791(Laminin G)|interpro:IPR002350(Proteinase inhibitor I1, Kazal)|interpro:IPR000082(SEA)|go:"GO:0043113"(receptor clustering)|go:"GO:0043236"(laminin binding)|go:"GO:0043237"(laminin-1 binding)|go:"GO:0043395"(heparan sulfate proteoglycan binding)|go:"GO:0043547"(positive regulation of GTPase activity)|go:"GO:0045121"(membrane raft)|go:"GO:0045178"(basal part of cell)|go:"GO:0045202"(synapse)|go:"GO:0045887"(positive regulation of synaptic assembly at neuromuscular junction)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046847"(filopodium assembly)|go:"GO:0050840"(extracellular matrix binding)|go:"GO:0051491"(positive regulation of filopodium assembly)|go:"GO:0071340"(skeletal muscle acetylcholine-gated channel clustering)|go:"GO:0098684"(photoreceptor ribbon synapse)|go:"GO:0098965"(extracellular matrix of synaptic cleft)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0038023"(signaling receptor activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005539"(glycosaminoglycan binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005604"(basement membrane)|go:"GO:0005615"(extracellular space)|go:"GO:0005886"(plasma membrane)|go:"GO:0007158"(neuron cell-cell adhesion)|go:"GO:0007213"(G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007399"(nervous system development)|go:"GO:0007420"(brain development)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0008201"(heparin binding)|go:"GO:0010977"(negative regulation of neuron projection development)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030154"(cell differentiation)|go:"GO:0030297"(transmembrane receptor protein tyrosine kinase activator activity)|go:"GO:0030424"(axon)|go:"GO:0030548"(acetylcholine receptor regulator activity)|go:"GO:0031012"(extracellular matrix)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0031594"(neuromuscular junction)|go:"GO:0033691"(sialic acid binding)|go:"GO:0035374"(chondroitin sulfate binding)|go:"GO:0002162"(dystroglycan binding)|interpro:IPR036058|interpro:IPR036364	refseq:XP_006515055.2|ensembl:ENSMUSG00000002900(gene)|ensembl:ENSMUST00000002979(transcript)|ensembl:ENSMUST00000169088(transcript)|go:"GO:0005178"(integrin binding)|go:"GO:0005201"(extracellular matrix structural constituent)|go:"GO:0005576"(extracellular region)|go:"GO:0005604"(basement membrane)|go:"GO:0005606"(laminin-1 complex)|go:"GO:0005607"(laminin-2 complex)|go:"GO:0005615"(extracellular space)|go:"GO:0005634"(nucleus)|go:"GO:0007162"(negative regulation of cell adhesion)|go:"GO:0007566"(embryo implantation)|go:"GO:0007611"(learning or memory)|go:"GO:0009887"(animal organ morphogenesis)|go:"GO:0009888"(tissue development)|go:"GO:0016477"(cell migration)|go:"GO:0019899"(enzyme binding)|go:"GO:0021812"(neuronal-glial interaction involved in cerebral cortex radial glia guided migration)|go:"GO:0030335"(positive regulation of cell migration)|go:"GO:0031012"(extracellular matrix)|go:"GO:0031175"(neuron projection development)|go:"GO:0034446"(substrate adhesion-dependent cell spreading)|go:"GO:0042476"(odontogenesis)|go:"GO:0043208"(glycosphingolipid binding)|go:"GO:0043256"(laminin complex)|go:"GO:0043257"(laminin-8 complex)|go:"GO:0043259"(laminin-10 complex)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0062023"(collagen-containing extracellular matrix)|reactome:R-MMU-3000157|reactome:R-MMU-381426|reactome:R-MMU-8874081|go:"GO:0070831"(basement membrane assembly)|reactome:R-MMU-8957275|interpro:IPR000742(EGF-like, type 3)|interpro:IPR002049(EGF-like, laminin)|interpro:IPR008211(Laminin, N-terminal)|interpro:IPR013015(Laminin IV)|mint:P02469|rcsb pdb:4AQS|rcsb pdb:5MC9|refseq:XP_006515056.1	go:"GO:0030548"(acetylcholine receptor regulator activity)	comment:"Recombinant cAgrin(7) (7 aa insert, fig 1a) purified from transfected HEK 293 cells.  Recombinant cN25(7)Fc (cDNA construct that encodes an agrin fragment comprising the NH2-terminal region, fig 1a) purified from transfected COS-7 cells."	comment:Lamini-1 was purified from Engelbreth-holm-Swarm mouse tumor	figure legend:5b|comment:Agrin synthesized and secreted by cells of the chick retina bind to Laminin-1 which is mediated by the NH2-terminal end.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/05/02	2017/09/02	rogid:b+RsTdBVRG0NoC+sRNwneIPSA1M9031	rogid:cUuVqY2JMeRrS9sDYudPy7hSIL010090	intact-crc:4E9A56C97582B7BB|rigid:TAqAHNPK7SeYpj3QyQleGeUtmxg	false	sufficient binding region:1-196..196|125i radiolabel:?-?|fc-igg tag:?-?	-	-	-	psi-mi:"MI:1197"(radioimmunoassay)	psi-mi:"MI:1197"(radioimmunoassay)
uniprotkb:F1NUQ6	uniprotkb:P31696	intact:EBI-6663175|ensembl:ENSGALP00000012842	intact:EBI-457650|uniprotkb:Q90609|uniprotkb:Q90685	psi-mi:f1nuq6_chick(display_long)|uniprotkb:LAMB4(gene name)|psi-mi:LAMB4(display_short)	psi-mi:agrin_chick(display_long)|uniprotkb:AGRN(gene name)|psi-mi:AGRN(display_short)|uniprotkb:AGRIN(gene name synonym)	psi-mi:"MI:0892"(solid phase assay)	Denzer et al. (1997)	pubmed:9151673|imex:IM-25866	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1335"(HPIDb)	intact:EBI-6663185|imex:IM-25866-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSGALG00000007917(gene)|ensembl:ENSGALT00000012857(transcript)|go:"GO:0005604"(basement membrane)|go:"GO:0016020"(membrane)|interpro:IPR000742(EGF-like, type 3)|interpro:IPR002049(EGF-like, laminin)|interpro:IPR008211(Laminin, N-terminal)|interpro:IPR013015(Laminin IV)|refseq:NP_001186242.1	interpro:IPR001881(EGF-like calcium-binding)|interpro:IPR003884(Factor I membrane attack complex)|interpro:IPR008993(TIMP-like, OB-fold)|rcsb pdb:1PZ8|rcsb pdb:1PZ9|rcsb pdb:1JB3|rcsb pdb:1JC7|rcsb pdb:1PXU|rcsb pdb:1PZ7|rcsb pdb:1Q56|rcsb pdb:3I70|interpro:IPR004850(Agrin NtA)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR000152(Aspartic acid and asparagine hydroxylation site)|interpro:IPR000742(EGF-like, type 3)|interpro:IPR002049(EGF-like, laminin)|interpro:IPR003645(Follistatin-like, N-terminal)|interpro:IPR001791(Laminin G)|interpro:IPR002350(Proteinase inhibitor I1, Kazal)|interpro:IPR000082(SEA)|go:"GO:0043113"(receptor clustering)|go:"GO:0043236"(laminin binding)|go:"GO:0043237"(laminin-1 binding)|go:"GO:0043395"(heparan sulfate proteoglycan binding)|go:"GO:0043547"(positive regulation of GTPase activity)|go:"GO:0045121"(membrane raft)|go:"GO:0045178"(basal part of cell)|go:"GO:0045202"(synapse)|go:"GO:0045887"(positive regulation of synaptic assembly at neuromuscular junction)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046847"(filopodium assembly)|go:"GO:0050840"(extracellular matrix binding)|go:"GO:0051491"(positive regulation of filopodium assembly)|go:"GO:0071340"(skeletal muscle acetylcholine-gated channel clustering)|go:"GO:0098684"(photoreceptor ribbon synapse)|go:"GO:0098965"(extracellular matrix of synaptic cleft)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0038023"(signaling receptor activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005539"(glycosaminoglycan binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005604"(basement membrane)|go:"GO:0005615"(extracellular space)|go:"GO:0005886"(plasma membrane)|go:"GO:0007158"(neuron cell-cell adhesion)|go:"GO:0007213"(G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007399"(nervous system development)|go:"GO:0007420"(brain development)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0008201"(heparin binding)|go:"GO:0010977"(negative regulation of neuron projection development)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030154"(cell differentiation)|go:"GO:0030297"(transmembrane receptor protein tyrosine kinase activator activity)|go:"GO:0030424"(axon)|go:"GO:0030548"(acetylcholine receptor regulator activity)|go:"GO:0031012"(extracellular matrix)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0031594"(neuromuscular junction)|go:"GO:0033691"(sialic acid binding)|go:"GO:0035374"(chondroitin sulfate binding)|go:"GO:0002162"(dystroglycan binding)|interpro:IPR036058|interpro:IPR036364	go:"GO:0030548"(acetylcholine receptor regulator activity)	comment:Laminin-4 isolated from adult chick heart	comment:"Recombinant cAgrin(7) (7 aa insert, fig 1a) purified from transfected HEK 293 cells.  Recombinant cN25(7)Fc (cDNA construct that encodes an agrin fragment comprising the NH2-terminal region, fig 1a) purified from transfected COS-7 cells."	figure legend:7|comment:"Both constructs of Agrin (cAgrin(7) and cN25(7)Fc) interact with Laminin-4"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/05/02	2017/09/02	rogid:Rfl6fpk7rhUwuhF67ykkdPJmSF09031	rogid:b+RsTdBVRG0NoC+sRNwneIPSA1M9031	intact-crc:18EA2BD4D1D053BD|rigid:64EypbrRZ2sb6YRJDpYThdU1+vU	false	-	125i radiolabel:?-?	-	-	psi-mi:"MI:1197"(radioimmunoassay)	psi-mi:"MI:1197"(radioimmunoassay)
intact:EBI-6663131	uniprotkb:P31696	-	intact:EBI-457650|uniprotkb:Q90609|uniprotkb:Q90685	psi-mi:f1p420_chick(display_short)|psi-mi:EBI-6663131(display_long)|uniprotkb:Gga.55092(gene name)|psi-mi:Gga.55092(display_short)	psi-mi:agrin_chick(display_long)|uniprotkb:AGRN(gene name)|psi-mi:AGRN(display_short)|uniprotkb:AGRIN(gene name synonym)	psi-mi:"MI:0892"(solid phase assay)	Denzer et al. (1997)	pubmed:9151673|imex:IM-25866	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1335"(HPIDb)	intact:EBI-6663102|imex:IM-25866-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	uniprotkb:F1P420(uniprot-removed-ac)	interpro:IPR001881(EGF-like calcium-binding)|interpro:IPR003884(Factor I membrane attack complex)|interpro:IPR008993(TIMP-like, OB-fold)|rcsb pdb:1PZ8|rcsb pdb:1PZ9|rcsb pdb:1JB3|rcsb pdb:1JC7|rcsb pdb:1PXU|rcsb pdb:1PZ7|rcsb pdb:1Q56|rcsb pdb:3I70|interpro:IPR004850(Agrin NtA)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR000152(Aspartic acid and asparagine hydroxylation site)|interpro:IPR000742(EGF-like, type 3)|interpro:IPR002049(EGF-like, laminin)|interpro:IPR003645(Follistatin-like, N-terminal)|interpro:IPR001791(Laminin G)|interpro:IPR002350(Proteinase inhibitor I1, Kazal)|interpro:IPR000082(SEA)|go:"GO:0043113"(receptor clustering)|go:"GO:0043236"(laminin binding)|go:"GO:0043237"(laminin-1 binding)|go:"GO:0043395"(heparan sulfate proteoglycan binding)|go:"GO:0043547"(positive regulation of GTPase activity)|go:"GO:0045121"(membrane raft)|go:"GO:0045178"(basal part of cell)|go:"GO:0045202"(synapse)|go:"GO:0045887"(positive regulation of synaptic assembly at neuromuscular junction)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046847"(filopodium assembly)|go:"GO:0050840"(extracellular matrix binding)|go:"GO:0051491"(positive regulation of filopodium assembly)|go:"GO:0071340"(skeletal muscle acetylcholine-gated channel clustering)|go:"GO:0098684"(photoreceptor ribbon synapse)|go:"GO:0098965"(extracellular matrix of synaptic cleft)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0038023"(signaling receptor activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005539"(glycosaminoglycan binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005604"(basement membrane)|go:"GO:0005615"(extracellular space)|go:"GO:0005886"(plasma membrane)|go:"GO:0007158"(neuron cell-cell adhesion)|go:"GO:0007213"(G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007399"(nervous system development)|go:"GO:0007420"(brain development)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0008201"(heparin binding)|go:"GO:0010977"(negative regulation of neuron projection development)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030154"(cell differentiation)|go:"GO:0030297"(transmembrane receptor protein tyrosine kinase activator activity)|go:"GO:0030424"(axon)|go:"GO:0030548"(acetylcholine receptor regulator activity)|go:"GO:0031012"(extracellular matrix)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0031594"(neuromuscular junction)|go:"GO:0033691"(sialic acid binding)|go:"GO:0035374"(chondroitin sulfate binding)|go:"GO:0002162"(dystroglycan binding)|interpro:IPR036058|interpro:IPR036364	go:"GO:0030548"(acetylcholine receptor regulator activity)	no-uniprot-update:|caution:The sequence has been withdrawn from uniprot.|comment:Laminin-2 isolated from adult chick heart	comment:"Recombinant cAgrin(7) (7 aa insert, fig 1a) purified from transfected HEK 293 cells.  Recombinant cN25(7)Fc (cDNA construct that encodes an agrin fragment comprising the NH2-terminal region, fig 1a) purified from transfected COS-7 cells."	figure legend:7|comment:"Both constructs of Agrin (cAgrin(7) and cN25(7)Fc) interact with Laminin-2"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/05/02	2017/09/02	rogid:kQvwFliwt7Qtbd0PGQKJm9tAGrQ9031	rogid:b+RsTdBVRG0NoC+sRNwneIPSA1M9031	intact-crc:E81BCE487F61EB60|rigid:02CvRYk434yiCHXgn8yuSi9du8E	false	-	125i radiolabel:?-?	-	-	psi-mi:"MI:1197"(radioimmunoassay)	psi-mi:"MI:1197"(radioimmunoassay)