#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P50281	uniprotkb:P16035	intact:EBI-992788|uniprotkb:Q6GSF3|uniprotkb:Q92678|uniprotkb:A8K5L0|ensembl:ENSP00000308208	intact:EBI-1033507|uniprotkb:Q16121|uniprotkb:Q93006|uniprotkb:Q9UDF7|ensembl:ENSP00000262768	psi-mi:mmp14_human(display_long)|uniprotkb:Membrane-type matrix metalloproteinase 1(gene name synonym)|uniprotkb:Membrane-type-1 matrix metalloproteinase(gene name synonym)|uniprotkb:MMP-X1(gene name synonym)|uniprotkb:MMP14(gene name)|psi-mi:MMP14(display_short)	psi-mi:timp2_human(display_long)|uniprotkb:TIMP2(gene name)|psi-mi:TIMP2(display_short)|uniprotkb:Tissue inhibitor of metalloproteinases 2(gene name synonym)|uniprotkb:CSC-21K(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Niarakis et al. (2013)	pubmed:24112707|imex:IM-21690	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8769407|imex:IM-21690-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENST00000311852(transcript)|go:"GO:0001501"(skeletal system development)|go:"GO:0001525"(angiogenesis)|go:"GO:0001541"(ovarian follicle development)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001935"(endothelial cell proliferation)|go:"GO:0001958"(endochondral ossification)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0004252"(serine-type endopeptidase activity)|go:"GO:0005178"(integrin binding)|go:"GO:0005615"(extracellular space)|go:"GO:0005634"(nucleus)|go:"GO:0005796"(Golgi lumen)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006508"(proteolysis)|go:"GO:0006979"(response to oxidative stress)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008584"(male gonad development)|go:"GO:0009612"(response to mechanical stimulus)|go:"GO:0010831"(positive regulation of myotube differentiation)|ensembl:ENSG00000157227(gene)|go:"GO:0010954"(positive regulation of protein processing)|go:"GO:0014070"(response to organic cyclic compound)|go:"GO:0016485"(protein processing)|go:"GO:0022617"(extracellular matrix disassembly)|go:"GO:0030198"(extracellular matrix organization)|go:"GO:0030307"(positive regulation of cell growth)|go:"GO:0030324"(lung development)|go:"GO:0030335"(positive regulation of cell migration)|go:"GO:0030574"(collagen catabolic process)|go:"GO:0031012"(extracellular matrix)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031638"(zymogen activation)|go:"GO:0035987"(endodermal cell differentiation)|go:"GO:0035988"(chondrocyte proliferation)|go:"GO:0042470"(melanosome)|go:"GO:0043615"(astrocyte cell migration)|go:"GO:0043627"(response to estrogen)|go:"GO:0044354"(macropinosome)|go:"GO:0045111"(intermediate filament cytoskeleton)|go:"GO:0045579"(positive regulation of B cell differentiation)|go:"GO:0045746"(negative regulation of Notch signaling pathway)|go:"GO:0048701"(embryonic cranial skeleton morphogenesis)|go:"GO:0048754"(branching morphogenesis of an epithelial tube)|go:"GO:0048771"(tissue remodeling)|go:"GO:0048870"(cell motility)|go:"GO:0051895"(negative regulation of focal adhesion assembly)|go:"GO:0060322"(head development)|go:"GO:0070006"(metalloaminopeptidase activity)|go:"GO:0097094"(craniofacial suture morphogenesis)|go:"GO:1905523"(positive regulation of macrophage migration)|go:"GO:1990834"(response to odorant)|interpro:IPR000585(Hemopexin)|go:"GO:1903076"(regulation of protein localization to plasma membrane)|interpro:IPR001818(Peptidase M10A and M12B, matrixin and adamalysin)|interpro:IPR002477(Peptidoglycan binding-like)|interpro:IPR006026(Peptidase, metallopeptidases)|interpro:IPR018486|interpro:IPR018487|interpro:IPR021158|interpro:IPR021190|interpro:IPR021805|interpro:IPR024079|interpro:IPR033739|interpro:IPR036365|interpro:IPR036366|interpro:IPR036375|mint:P50281|rcsb pdb:1BQQ|rcsb pdb:1BUV|rcsb pdb:2MQS|rcsb pdb:3MA2|rcsb pdb:3X23|rcsb pdb:4P3C|rcsb pdb:4P3D|rcsb pdb:4QXU|rcsb pdb:5H0U|rcsb pdb:6CLZ|rcsb pdb:6CM1|reactome:R-HSA-1442490|reactome:R-HSA-1474228|reactome:R-HSA-1592389|rcsb pdb:3C7X|refseq:NP_004986.1|dip:DIP-35111N	interpro:IPR015613(Tissue inhibitor of metalloprotease 2)|go:"GO:0062023"(collagen-containing extracellular matrix)|interpro:IPR027465|interpro:IPR030490|rcsb pdb:1BR9|rcsb pdb:1GXD|rcsb pdb:2TMP|rcsb pdb:4ILW|reactome:R-HSA-1592389|reactome:R-HSA-6798695|go:"GO:1904724"(tertiary granule lumen)|go:"GO:1904813"(ficolin-1-rich granule lumen)|go:"GO:1905049"(negative regulation of metallopeptidase activity)|interpro:IPR001134(Netrin, C-terminal)|interpro:IPR001820(Proteinase inhibitor I35, tissue inhibitor of metalloproteinase)|interpro:IPR008993(TIMP-like, OB-fold)|refseq:NP_003246.1|ensembl:ENSG00000035862(gene)|ensembl:ENST00000262768(transcript)|go:"GO:0002020"(protease binding)|go:"GO:0005178"(integrin binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0007417"(central nervous system development)|go:"GO:0007568"(aging)|go:"GO:0008191"(metalloendopeptidase inhibitor activity)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0009725"(response to hormone)|go:"GO:0009986"(cell surface)|go:"GO:0010033"(response to organic substance)|go:"GO:0010951"(negative regulation of endopeptidase activity)|go:"GO:0030414"(peptidase inhibitor activity)|go:"GO:0030426"(growth cone)|go:"GO:0031012"(extracellular matrix)|go:"GO:0032487"(regulation of Rap protein signal transduction)|go:"GO:0034097"(response to cytokine)|go:"GO:0035580"(specific granule lumen)|go:"GO:0042493"|go:"GO:0043025"(neuronal cell body)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045666"(positive regulation of neuron differentiation)|go:"GO:0045762"(positive regulation of adenylate cyclase activity)|go:"GO:0045930"(negative regulation of mitotic cell cycle)|go:"GO:0046580"(negative regulation of Ras protein signal transduction)|go:"GO:0051045"(negative regulation of membrane protein ectodomain proteolysis)	-	-	-	figure legend:f5a|comment:"However, upon immunoprecipitation of sMT1-MMP from tissue extracts and fluids with  polyclonal antibodies against the hinge region of MT1-MMP and western blot analysis, using  monoclonal antibodies against human TIMP-2, an immunoreactive band of ~ 21 kDa, migrating  as the human recombinant TIMP-2, was observed in all samples (Fig. 5A). Since polyclonal  antibodies against the hinge region of MT1-MMP used for immunoprecipitation, do not  recognize TIMP-2 (Fig. 5B), and they did not show non specific reaction with monoclonal  antibodies against TIMP-2 (Fig. 5A), these results suggest that TIMP-2 co-precipitated with  sMT1-MMP because it was already in complex with it. Consequently, it appears that the sMT1-  MMP in tissue extracts and fluids exists in a latent form and yet, bound to TIMP-2.  These results were further confirmed by reverse immunoprecipitation with monoclonal  antibodies against TIMP-2 and western blotting using polyclonal antibodies against the hinge region of MT1-MMP. Only one immunoreactive band of ~ 57 kDa, corresponding to sMT1-  MMP, was observed in all samples (Fig. 5C)."|comment:"The same interaction has been detected in the following tissues:  SF-OA, SF-RA, PSSF, POL, LUNG, PCT, IFT."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human)|taxid:9606(Homo sapiens)	-	2013/09/23	2014/10/16	rogid:x2H2pYgnfBvYy7Stikq3uk+ihhw9606	rogid:CjbiuqYHNikoKhO8OfAJKNCu4S49606	intact-crc:3DFE47FDAE8E0264|rigid:gXcbBBhA1QyBtGSQCEcMq6DRKMg	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0113"(western blot)
uniprotkb:P50281	uniprotkb:P16035	intact:EBI-992788|uniprotkb:Q6GSF3|uniprotkb:Q92678|uniprotkb:A8K5L0|ensembl:ENSP00000308208	intact:EBI-1033507|uniprotkb:Q16121|uniprotkb:Q93006|uniprotkb:Q9UDF7|ensembl:ENSP00000262768	psi-mi:mmp14_human(display_long)|uniprotkb:Membrane-type matrix metalloproteinase 1(gene name synonym)|uniprotkb:Membrane-type-1 matrix metalloproteinase(gene name synonym)|uniprotkb:MMP-X1(gene name synonym)|uniprotkb:MMP14(gene name)|psi-mi:MMP14(display_short)	psi-mi:timp2_human(display_long)|uniprotkb:TIMP2(gene name)|psi-mi:TIMP2(display_short)|uniprotkb:Tissue inhibitor of metalloproteinases 2(gene name synonym)|uniprotkb:CSC-21K(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Niarakis et al. (2013)	pubmed:24112707|imex:IM-21690	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8769421|imex:IM-21690-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENST00000311852(transcript)|go:"GO:0001501"(skeletal system development)|go:"GO:0001525"(angiogenesis)|go:"GO:0001541"(ovarian follicle development)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001935"(endothelial cell proliferation)|go:"GO:0001958"(endochondral ossification)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0004252"(serine-type endopeptidase activity)|go:"GO:0005178"(integrin binding)|go:"GO:0005615"(extracellular space)|go:"GO:0005634"(nucleus)|go:"GO:0005796"(Golgi lumen)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006508"(proteolysis)|go:"GO:0006979"(response to oxidative stress)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008584"(male gonad development)|go:"GO:0009612"(response to mechanical stimulus)|go:"GO:0010831"(positive regulation of myotube differentiation)|ensembl:ENSG00000157227(gene)|go:"GO:0010954"(positive regulation of protein processing)|go:"GO:0014070"(response to organic cyclic compound)|go:"GO:0016485"(protein processing)|go:"GO:0022617"(extracellular matrix disassembly)|go:"GO:0030198"(extracellular matrix organization)|go:"GO:0030307"(positive regulation of cell growth)|go:"GO:0030324"(lung development)|go:"GO:0030335"(positive regulation of cell migration)|go:"GO:0030574"(collagen catabolic process)|go:"GO:0031012"(extracellular matrix)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031638"(zymogen activation)|go:"GO:0035987"(endodermal cell differentiation)|go:"GO:0035988"(chondrocyte proliferation)|go:"GO:0042470"(melanosome)|go:"GO:0043615"(astrocyte cell migration)|go:"GO:0043627"(response to estrogen)|go:"GO:0044354"(macropinosome)|go:"GO:0045111"(intermediate filament cytoskeleton)|go:"GO:0045579"(positive regulation of B cell differentiation)|go:"GO:0045746"(negative regulation of Notch signaling pathway)|go:"GO:0048701"(embryonic cranial skeleton morphogenesis)|go:"GO:0048754"(branching morphogenesis of an epithelial tube)|go:"GO:0048771"(tissue remodeling)|go:"GO:0048870"(cell motility)|go:"GO:0051895"(negative regulation of focal adhesion assembly)|go:"GO:0060322"(head development)|go:"GO:0070006"(metalloaminopeptidase activity)|go:"GO:0097094"(craniofacial suture morphogenesis)|go:"GO:1905523"(positive regulation of macrophage migration)|go:"GO:1990834"(response to odorant)|interpro:IPR000585(Hemopexin)|go:"GO:1903076"(regulation of protein localization to plasma membrane)|interpro:IPR001818(Peptidase M10A and M12B, matrixin and adamalysin)|interpro:IPR002477(Peptidoglycan binding-like)|interpro:IPR006026(Peptidase, metallopeptidases)|interpro:IPR018486|interpro:IPR018487|interpro:IPR021158|interpro:IPR021190|interpro:IPR021805|interpro:IPR024079|interpro:IPR033739|interpro:IPR036365|interpro:IPR036366|interpro:IPR036375|mint:P50281|rcsb pdb:1BQQ|rcsb pdb:1BUV|rcsb pdb:2MQS|rcsb pdb:3MA2|rcsb pdb:3X23|rcsb pdb:4P3C|rcsb pdb:4P3D|rcsb pdb:4QXU|rcsb pdb:5H0U|rcsb pdb:6CLZ|rcsb pdb:6CM1|reactome:R-HSA-1442490|reactome:R-HSA-1474228|reactome:R-HSA-1592389|rcsb pdb:3C7X|refseq:NP_004986.1|dip:DIP-35111N	interpro:IPR015613(Tissue inhibitor of metalloprotease 2)|go:"GO:0062023"(collagen-containing extracellular matrix)|interpro:IPR027465|interpro:IPR030490|rcsb pdb:1BR9|rcsb pdb:1GXD|rcsb pdb:2TMP|rcsb pdb:4ILW|reactome:R-HSA-1592389|reactome:R-HSA-6798695|go:"GO:1904724"(tertiary granule lumen)|go:"GO:1904813"(ficolin-1-rich granule lumen)|go:"GO:1905049"(negative regulation of metallopeptidase activity)|interpro:IPR001134(Netrin, C-terminal)|interpro:IPR001820(Proteinase inhibitor I35, tissue inhibitor of metalloproteinase)|interpro:IPR008993(TIMP-like, OB-fold)|refseq:NP_003246.1|ensembl:ENSG00000035862(gene)|ensembl:ENST00000262768(transcript)|go:"GO:0002020"(protease binding)|go:"GO:0005178"(integrin binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0007417"(central nervous system development)|go:"GO:0007568"(aging)|go:"GO:0008191"(metalloendopeptidase inhibitor activity)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0009725"(response to hormone)|go:"GO:0009986"(cell surface)|go:"GO:0010033"(response to organic substance)|go:"GO:0010951"(negative regulation of endopeptidase activity)|go:"GO:0030414"(peptidase inhibitor activity)|go:"GO:0030426"(growth cone)|go:"GO:0031012"(extracellular matrix)|go:"GO:0032487"(regulation of Rap protein signal transduction)|go:"GO:0034097"(response to cytokine)|go:"GO:0035580"(specific granule lumen)|go:"GO:0042493"|go:"GO:0043025"(neuronal cell body)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045666"(positive regulation of neuron differentiation)|go:"GO:0045762"(positive regulation of adenylate cyclase activity)|go:"GO:0045930"(negative regulation of mitotic cell cycle)|go:"GO:0046580"(negative regulation of Ras protein signal transduction)|go:"GO:0051045"(negative regulation of membrane protein ectodomain proteolysis)	-	-	-	figure legend:f5C|comment:"However, upon immunoprecipitation of sMT1-MMP from tissue extracts and fluids with  polyclonal antibodies against the hinge region of MT1-MMP and western blot analysis, using  monoclonal antibodies against human TIMP-2, an immunoreactive band of ~ 21 kDa, migrating  as the human recombinant TIMP-2, was observed in all samples (Fig. 5A). Since polyclonal  antibodies against the hinge region of MT1-MMP used for immunoprecipitation, do not  recognize TIMP-2 (Fig. 5B), and they did not show non specific reaction with monoclonal  antibodies against TIMP-2 (Fig. 5A), these results suggest that TIMP-2 co-precipitated with  sMT1-MMP because it was already in complex with it. Consequently, it appears that the sMT1-  MMP in tissue extracts and fluids exists in a latent form and yet, bound to TIMP-2.  These results were further confirmed by reverse immunoprecipitation with monoclonal  antibodies against TIMP-2 and western blotting using polyclonal antibodies against the hinge region of MT1-MMP. Only one immunoreactive band of ~ 57 kDa, corresponding to sMT1-  MMP, was observed in all samples (Fig. 5C)."|comment:"The same interaction has been detected in the following tissues:  SF-OA, SF-RA, PSSF, POL, PCT, IFT."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human)|taxid:9606(Homo sapiens)	-	2013/09/23	2014/10/16	rogid:x2H2pYgnfBvYy7Stikq3uk+ihhw9606	rogid:CjbiuqYHNikoKhO8OfAJKNCu4S49606	intact-crc:330F6D1EF3700569|rigid:gXcbBBhA1QyBtGSQCEcMq6DRKMg	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0396"(predetermined participant)