#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
chebi:"CHEBI:15422"	uniprotkb:P0AEF0	intact:EBI-1108845|chebi:"CHEBI:40938"|chebi:"CHEBI:10841"|chebi:"CHEBI:10789"|chebi:"CHEBI:2359"|chebi:"CHEBI:13236"|chebi:"CHEBI:22249"	intact:EBI-549012|uniprotkb:P07905|uniprotkb:Q2M5V5|ensemblbacteria:AAC77317|ensemblbacteria:BAE78351	psi-mi:atp(display_short)|psi-mi:"CHEBI:15422"(display_long)|intact:Adenosine 5'-triphosphate(synonym)|intact:Adenosine triphosphate(synonym)|intact:ADENOSINE-5'-TRIPHOSPHATE(synonym)|intact:Adephos(synonym)|intact:Adetol(synonym)|intact:Adynol(synonym)|intact:Atipi(synonym)|intact:ATP(synonym)|intact:Atriphos(synonym)|intact:Cardenosine(synonym)|intact:Fosfobion(synonym)|intact:Glucobasin(synonym)|intact:H4atp(synonym)|intact:Myotriphos(synonym)|intact:Triadenyl(synonym)|intact:Triphosphaden(synonym)|intact:"adenosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:dnac_ecoli(display_long)|uniprotkb:dnaC(gene name)|psi-mi:dnaC(display_short)|uniprotkb:dnaD(gene name synonym)|uniprotkb:b4361(locus name)|uniprotkb:JW4325(locus name)	psi-mi:"MI:0030"(cross-linking study)	Makowska-Grzyska et al. (2010)	pubmed:20129058|imex:IM-18644	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6504892|imex:IM-18644-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:NP_418781.1|refseq:WP_000799911.1|dip:DIP-9457N|ensemblbacteria:AAC77317(transcript)|ensemblbacteria:b4361(gene)|ensemblbacteria:BAE78351(gene)|ensemblbacteria:BAE78351(transcript)|go:"GO:0005524"(ATP binding)|go:"GO:0006260"(DNA replication)|go:"GO:0006269"(DNA replication, synthesis of RNA primer)|go:"GO:0006271"(DNA strand elongation involved in DNA replication)|go:"GO:1990077"(primosome complex)|interpro:IPR002611(IstB-like ATP-binding protein)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR027417|interpro:IPR028350|rcsb pdb:6KZA|rcsb pdb:6QEL|rcsb pdb:6QEM	-	-	-	figure legend:Fig. 2a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/01/30	2014/10/16	-	rogid:JuiZiIQR0NdZ0Dm8/F/baTwL89A83333	intact-crc:7E6E9AA0CE808644	false	32p radiolabel:?-?	-	-	-	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)
uniprotkb:P0ACB0	uniprotkb:P0AEF0	intact:EBI-548978|uniprotkb:P03005|uniprotkb:Q2M6Q2|ensemblbacteria:AAC77022|ensemblbacteria:BAE78054	intact:EBI-549012|uniprotkb:P07905|uniprotkb:Q2M5V5|ensemblbacteria:AAC77317|ensemblbacteria:BAE78351	psi-mi:dnab_ecoli(display_long)|uniprotkb:dnaB(gene name)|psi-mi:dnaB(display_short)|uniprotkb:groP(gene name synonym)|uniprotkb:grpA(gene name synonym)|uniprotkb:b4052(locus name)|uniprotkb:JW4012(locus name)	psi-mi:dnac_ecoli(display_long)|uniprotkb:dnaC(gene name)|psi-mi:dnaC(display_short)|uniprotkb:dnaD(gene name synonym)|uniprotkb:b4361(locus name)|uniprotkb:JW4325(locus name)	psi-mi:"MI:0049"(filter binding)	Makowska-Grzyska et al. (2010)	pubmed:20129058|imex:IM-18644	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6504940|imex:IM-18644-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_418476.1|refseq:WP_000918363.1|dip:DIP-9456N|ensemblbacteria:AAC77022(transcript)|ensemblbacteria:b4052(gene)|ensemblbacteria:BAE78054(gene)|ensemblbacteria:BAE78054(transcript)|go:"GO:0003677"(DNA binding)|go:"GO:0003678"(DNA helicase activity)|go:"GO:0004386"(helicase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0006260"(DNA replication)|go:"GO:0006268"(DNA unwinding involved in DNA replication)|go:"GO:0006269"(DNA replication, synthesis of RNA primer)|go:"GO:0010212"(response to ionizing radiation)|go:"GO:0042802"(identical protein binding)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1990077"(primosome complex)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR007692(DNA helicase, DnaB type)|interpro:IPR007693(DNA helicase, DnaB-like, N-terminal)|interpro:IPR007694(DNA helicase, DnaB-like, C-terminal)|interpro:IPR016136(DNA helicase DnaB, N-terminal/DNA primase DnaG, C-terminal)|interpro:IPR027417|interpro:IPR036185|rcsb pdb:1B79|rcsb pdb:1JWE|rcsb pdb:6BBM|rcsb pdb:6KZA|rcsb pdb:6QEL|rcsb pdb:6QEM	refseq:NP_418781.1|refseq:WP_000799911.1|dip:DIP-9457N|ensemblbacteria:AAC77317(transcript)|ensemblbacteria:b4361(gene)|ensemblbacteria:BAE78351(gene)|ensemblbacteria:BAE78351(transcript)|go:"GO:0005524"(ATP binding)|go:"GO:0006260"(DNA replication)|go:"GO:0006269"(DNA replication, synthesis of RNA primer)|go:"GO:0006271"(DNA strand elongation involved in DNA replication)|go:"GO:1990077"(primosome complex)|interpro:IPR002611(IstB-like ATP-binding protein)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR027417|interpro:IPR028350|rcsb pdb:6KZA|rcsb pdb:6QEL|rcsb pdb:6QEM	go:"GO:0043234"|go:"GO:0006268"(DNA unwinding involved in DNA replication)	-	-	figure legend:Fig. 2b, c|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/01/30	2014/10/16	rogid:czmOnQQpFov7eFmCjLGjNhkbJ7M83333	rogid:JuiZiIQR0NdZ0Dm8/F/baTwL89A83333	intact-crc:29339F058DA74A63|rigid:c/jDjri0ft8uf5qqQUab2q2FJIU	false	-	mutation disrupting interaction:11-11	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
intact:EBI-6505013	uniprotkb:P0AEF0	-	intact:EBI-549012|uniprotkb:P07905|uniprotkb:Q2M5V5|ensemblbacteria:AAC77317|ensemblbacteria:BAE78351	psi-mi:ssdna(display_short)|psi-mi:EBI-6505013(display_long)	psi-mi:dnac_ecoli(display_long)|uniprotkb:dnaC(gene name)|psi-mi:dnaC(display_short)|uniprotkb:dnaD(gene name synonym)|uniprotkb:b4361(locus name)|uniprotkb:JW4325(locus name)	psi-mi:"MI:0107"(surface plasmon resonance)	Makowska-Grzyska et al. (2010)	pubmed:20129058|imex:IM-18644	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6504997|imex:IM-18644-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	refseq:NP_418781.1|refseq:WP_000799911.1|dip:DIP-9457N|ensemblbacteria:AAC77317(transcript)|ensemblbacteria:b4361(gene)|ensemblbacteria:BAE78351(gene)|ensemblbacteria:BAE78351(transcript)|go:"GO:0005524"(ATP binding)|go:"GO:0006260"(DNA replication)|go:"GO:0006269"(DNA replication, synthesis of RNA primer)|go:"GO:0006271"(DNA strand elongation involved in DNA replication)|go:"GO:1990077"(primosome complex)|interpro:IPR002611(IstB-like ATP-binding protein)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR027417|interpro:IPR028350|rcsb pdb:6KZA|rcsb pdb:6QEL|rcsb pdb:6QEM	-	-	-	figure legend:Fig. 3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:3.3x10^-6(molar)	2013/01/30	2014/10/16	-	rogid:JuiZiIQR0NdZ0Dm8/F/baTwL89A83333	intact-crc:9FAD9019798325FE	false	biotin tag:1-1	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:15422"	intact:EBI-6505013	intact:EBI-1108845|chebi:"CHEBI:40938"|chebi:"CHEBI:10841"|chebi:"CHEBI:10789"|chebi:"CHEBI:2359"|chebi:"CHEBI:13236"|chebi:"CHEBI:22249"	-	psi-mi:atp(display_short)|psi-mi:"CHEBI:15422"(display_long)|intact:Adenosine 5'-triphosphate(synonym)|intact:Adenosine triphosphate(synonym)|intact:ADENOSINE-5'-TRIPHOSPHATE(synonym)|intact:Adephos(synonym)|intact:Adetol(synonym)|intact:Adynol(synonym)|intact:Atipi(synonym)|intact:ATP(synonym)|intact:Atriphos(synonym)|intact:Cardenosine(synonym)|intact:Fosfobion(synonym)|intact:Glucobasin(synonym)|intact:H4atp(synonym)|intact:Myotriphos(synonym)|intact:Triadenyl(synonym)|intact:Triphosphaden(synonym)|intact:"adenosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:ssdna(display_short)|psi-mi:EBI-6505013(display_long)	psi-mi:"MI:0107"(surface plasmon resonance)	Makowska-Grzyska et al. (2010)	pubmed:20129058|imex:IM-18644	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6504997|imex:IM-18644-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	-	-	-	-	-	figure legend:Fig. 3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:3.3x10^-6(molar)	2013/01/30	2014/10/16	-	-	intact-crc:9FAD9019798325FE	false	-	biotin tag:1-1	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0AEF0	intact:EBI-6505013	intact:EBI-549012|uniprotkb:P07905|uniprotkb:Q2M5V5|ensemblbacteria:AAC77317|ensemblbacteria:BAE78351	-	psi-mi:dnac_ecoli(display_long)|uniprotkb:dnaC(gene name)|psi-mi:dnaC(display_short)|uniprotkb:dnaD(gene name synonym)|uniprotkb:b4361(locus name)|uniprotkb:JW4325(locus name)	psi-mi:ssdna(display_short)|psi-mi:EBI-6505013(display_long)	psi-mi:"MI:0107"(surface plasmon resonance)	Makowska-Grzyska et al. (2010)	pubmed:20129058|imex:IM-18644	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6505041|imex:IM-18644-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	refseq:NP_418781.1|refseq:WP_000799911.1|dip:DIP-9457N|ensemblbacteria:AAC77317(transcript)|ensemblbacteria:b4361(gene)|ensemblbacteria:BAE78351(gene)|ensemblbacteria:BAE78351(transcript)|go:"GO:0005524"(ATP binding)|go:"GO:0006260"(DNA replication)|go:"GO:0006269"(DNA replication, synthesis of RNA primer)|go:"GO:0006271"(DNA strand elongation involved in DNA replication)|go:"GO:1990077"(primosome complex)|interpro:IPR002611(IstB-like ATP-binding protein)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR027417|interpro:IPR028350|rcsb pdb:6KZA|rcsb pdb:6QEL|rcsb pdb:6QEM	-	-	-	-	figure legend:Fig. 3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:7.9x10^-6(molar)	2013/01/30	2014/10/16	rogid:JuiZiIQR0NdZ0Dm8/F/baTwL89A83333	-	intact-crc:A2D82C094BBCD393	false	-	biotin tag:1-1	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P03004	uniprotkb:P0ACB0	intact:EBI-548951|uniprotkb:P78122|uniprotkb:Q2M814|ensemblbacteria:AAC76725|ensemblbacteria:BAE77592	intact:EBI-548978|uniprotkb:P03005|uniprotkb:Q2M6Q2|ensemblbacteria:AAC77022|ensemblbacteria:BAE78054	psi-mi:dnaa_ecoli(display_long)|uniprotkb:dnaA(gene name)|psi-mi:dnaA(display_short)|uniprotkb:b3702(locus name)|uniprotkb:JW3679(locus name)	psi-mi:dnab_ecoli(display_long)|uniprotkb:dnaB(gene name)|psi-mi:dnaB(display_short)|uniprotkb:groP(gene name synonym)|uniprotkb:grpA(gene name synonym)|uniprotkb:b4052(locus name)|uniprotkb:JW4012(locus name)	psi-mi:"MI:0808"(comigration in sds page)	Makowska-Grzyska et al. (2010)	pubmed:20129058|imex:IM-18644	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6505446|imex:IM-18644-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_418157.1|refseq:WP_000059111.1|dip:DIP-9455N|ensemblbacteria:AAC76725(transcript)|ensemblbacteria:b3702(gene)|ensemblbacteria:BAE77592(gene)|ensemblbacteria:BAE77592(transcript)|go:"GO:0003677"(DNA binding)|go:"GO:0003688"(DNA replication origin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006260"(DNA replication)|go:"GO:0006270"(DNA replication initiation)|go:"GO:0006275"(regulation of DNA replication)|go:"GO:0008156"(negative regulation of DNA replication)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043565"(sequence-specific DNA binding)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR010921(Trp repressor/replication initiator)|interpro:IPR013159(Chromosomal replication initiator, DnaA C-terminal)|interpro:IPR013317(Chromosomal replication initiator, DnaA)|interpro:IPR018312|interpro:IPR020591|interpro:IPR024633|interpro:IPR027417|interpro:IPR038454|rcsb pdb:1J1V|rcsb pdb:2E0G|interpro:IPR001957(Bacterial chromosomal replication initiator protein, DnaA)	refseq:NP_418476.1|refseq:WP_000918363.1|dip:DIP-9456N|ensemblbacteria:AAC77022(transcript)|ensemblbacteria:b4052(gene)|ensemblbacteria:BAE78054(gene)|ensemblbacteria:BAE78054(transcript)|go:"GO:0003677"(DNA binding)|go:"GO:0003678"(DNA helicase activity)|go:"GO:0004386"(helicase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0006260"(DNA replication)|go:"GO:0006268"(DNA unwinding involved in DNA replication)|go:"GO:0006269"(DNA replication, synthesis of RNA primer)|go:"GO:0010212"(response to ionizing radiation)|go:"GO:0042802"(identical protein binding)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1990077"(primosome complex)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR007692(DNA helicase, DnaB type)|interpro:IPR007693(DNA helicase, DnaB-like, N-terminal)|interpro:IPR007694(DNA helicase, DnaB-like, C-terminal)|interpro:IPR016136(DNA helicase DnaB, N-terminal/DNA primase DnaG, C-terminal)|interpro:IPR027417|interpro:IPR036185|rcsb pdb:1B79|rcsb pdb:1JWE|rcsb pdb:6BBM|rcsb pdb:6KZA|rcsb pdb:6QEL|rcsb pdb:6QEM	go:"GO:0043234"|go:"GO:0006268"(DNA unwinding involved in DNA replication)	-	-	figure legend:Fig. 5, 6|comment:"Stoichiometry of DnaA, DnaB and DnaC (wildtype) calculated relative to one copy of the oriC plasmid in an assay containing M13oriC2LB5, DnaA, DnaB, DnaC, HU, SSB and ATP/ATPgammaS. Stoichiometry changes slightly depending on which prepriming and replication complex enzymes were added to the assay (Fig. 6).   Binding of DnaC lost if DnaG (primase) and rNTPs added."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/01/30	2014/10/16	rogid:p07cXpfl7bzSh/kImTD/+Zr0Ma883333	rogid:czmOnQQpFov7eFmCjLGjNhkbJ7M83333	intact-crc:85DA3A96AECE39FC	false	-	-	9	11	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P03004	uniprotkb:P0AEF0	intact:EBI-548951|uniprotkb:P78122|uniprotkb:Q2M814|ensemblbacteria:AAC76725|ensemblbacteria:BAE77592	intact:EBI-549012|uniprotkb:P07905|uniprotkb:Q2M5V5|ensemblbacteria:AAC77317|ensemblbacteria:BAE78351	psi-mi:dnaa_ecoli(display_long)|uniprotkb:dnaA(gene name)|psi-mi:dnaA(display_short)|uniprotkb:b3702(locus name)|uniprotkb:JW3679(locus name)	psi-mi:dnac_ecoli(display_long)|uniprotkb:dnaC(gene name)|psi-mi:dnaC(display_short)|uniprotkb:dnaD(gene name synonym)|uniprotkb:b4361(locus name)|uniprotkb:JW4325(locus name)	psi-mi:"MI:0808"(comigration in sds page)	Makowska-Grzyska et al. (2010)	pubmed:20129058|imex:IM-18644	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6505446|imex:IM-18644-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_418157.1|refseq:WP_000059111.1|dip:DIP-9455N|ensemblbacteria:AAC76725(transcript)|ensemblbacteria:b3702(gene)|ensemblbacteria:BAE77592(gene)|ensemblbacteria:BAE77592(transcript)|go:"GO:0003677"(DNA binding)|go:"GO:0003688"(DNA replication origin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006260"(DNA replication)|go:"GO:0006270"(DNA replication initiation)|go:"GO:0006275"(regulation of DNA replication)|go:"GO:0008156"(negative regulation of DNA replication)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043565"(sequence-specific DNA binding)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR010921(Trp repressor/replication initiator)|interpro:IPR013159(Chromosomal replication initiator, DnaA C-terminal)|interpro:IPR013317(Chromosomal replication initiator, DnaA)|interpro:IPR018312|interpro:IPR020591|interpro:IPR024633|interpro:IPR027417|interpro:IPR038454|rcsb pdb:1J1V|rcsb pdb:2E0G|interpro:IPR001957(Bacterial chromosomal replication initiator protein, DnaA)	refseq:NP_418781.1|refseq:WP_000799911.1|dip:DIP-9457N|ensemblbacteria:AAC77317(transcript)|ensemblbacteria:b4361(gene)|ensemblbacteria:BAE78351(gene)|ensemblbacteria:BAE78351(transcript)|go:"GO:0005524"(ATP binding)|go:"GO:0006260"(DNA replication)|go:"GO:0006269"(DNA replication, synthesis of RNA primer)|go:"GO:0006271"(DNA strand elongation involved in DNA replication)|go:"GO:1990077"(primosome complex)|interpro:IPR002611(IstB-like ATP-binding protein)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR027417|interpro:IPR028350|rcsb pdb:6KZA|rcsb pdb:6QEL|rcsb pdb:6QEM	go:"GO:0043234"|go:"GO:0006268"(DNA unwinding involved in DNA replication)	-	-	figure legend:Fig. 5, 6|comment:"Stoichiometry of DnaA, DnaB and DnaC (wildtype) calculated relative to one copy of the oriC plasmid in an assay containing M13oriC2LB5, DnaA, DnaB, DnaC, HU, SSB and ATP/ATPgammaS. Stoichiometry changes slightly depending on which prepriming and replication complex enzymes were added to the assay (Fig. 6).   Binding of DnaC lost if DnaG (primase) and rNTPs added."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/01/30	2014/10/16	rogid:p07cXpfl7bzSh/kImTD/+Zr0Ma883333	rogid:JuiZiIQR0NdZ0Dm8/F/baTwL89A83333	intact-crc:85DA3A96AECE39FC	false	-	-	9	6	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P03004	intact:EBI-6505429	intact:EBI-548951|uniprotkb:P78122|uniprotkb:Q2M814|ensemblbacteria:AAC76725|ensemblbacteria:BAE77592	-	psi-mi:dnaa_ecoli(display_long)|uniprotkb:dnaA(gene name)|psi-mi:dnaA(display_short)|uniprotkb:b3702(locus name)|uniprotkb:JW3679(locus name)	psi-mi:m13oric2lb5(display_short)|psi-mi:EBI-6505429(display_long)	psi-mi:"MI:0808"(comigration in sds page)	Makowska-Grzyska et al. (2010)	pubmed:20129058|imex:IM-18644	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6505446|imex:IM-18644-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0319"(deoxyribonucleic acid)	refseq:NP_418157.1|refseq:WP_000059111.1|dip:DIP-9455N|ensemblbacteria:AAC76725(transcript)|ensemblbacteria:b3702(gene)|ensemblbacteria:BAE77592(gene)|ensemblbacteria:BAE77592(transcript)|go:"GO:0003677"(DNA binding)|go:"GO:0003688"(DNA replication origin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006260"(DNA replication)|go:"GO:0006270"(DNA replication initiation)|go:"GO:0006275"(regulation of DNA replication)|go:"GO:0008156"(negative regulation of DNA replication)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043565"(sequence-specific DNA binding)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR010921(Trp repressor/replication initiator)|interpro:IPR013159(Chromosomal replication initiator, DnaA C-terminal)|interpro:IPR013317(Chromosomal replication initiator, DnaA)|interpro:IPR018312|interpro:IPR020591|interpro:IPR024633|interpro:IPR027417|interpro:IPR038454|rcsb pdb:1J1V|rcsb pdb:2E0G|interpro:IPR001957(Bacterial chromosomal replication initiator protein, DnaA)	pubmed:20129058(method reference)	go:"GO:0043234"|go:"GO:0006268"(DNA unwinding involved in DNA replication)	-	-	figure legend:Fig. 5, 6|comment:"Stoichiometry of DnaA, DnaB and DnaC (wildtype) calculated relative to one copy of the oriC plasmid in an assay containing M13oriC2LB5, DnaA, DnaB, DnaC, HU, SSB and ATP/ATPgammaS. Stoichiometry changes slightly depending on which prepriming and replication complex enzymes were added to the assay (Fig. 6).   Binding of DnaC lost if DnaG (primase) and rNTPs added."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/01/30	2014/10/16	rogid:p07cXpfl7bzSh/kImTD/+Zr0Ma883333	-	intact-crc:85DA3A96AECE39FC	false	-	-	9	1	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)