#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P0A700	uniprotkb:P16431	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:"MI:0096"(pull down)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6290903|imex:IM-17822-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	-	-	-	figure legend:Figure 1B|comment:"HycE co-eluted with HypAStr in the unprocessed form (supplemental Fig. S1)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	intact-crc:46D210951C25954B|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0A700	uniprotkb:P0AAN3	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-558261|uniprotkb:P24190|uniprotkb:Q2MAA2|uniprotkb:Q46884|ensemblbacteria:AAC75769|ensemblbacteria:BAE76804	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:hypb_ecoli(display_long)|uniprotkb:hypB(gene name)|psi-mi:hypB(display_short)|uniprotkb:Hydrogenase isoenzymes nickel incorporation protein HypB(gene name synonym)|uniprotkb:JW2697(locus name)|uniprotkb:b2727(locus name)	psi-mi:"MI:0096"(pull down)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6290903|imex:IM-17822-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417207.1|refseq:WP_000337665.1|dip:DIP-36428N|ensemblbacteria:AAC75769(transcript)|ensemblbacteria:b2727(gene)|ensemblbacteria:BAE76804(gene)|ensemblbacteria:BAE76804(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0051604"(protein maturation)|interpro:IPR003495("Cobalamin (vitamin B12) biosynthesis CobW-like")|interpro:IPR004392(Hydrogenase accessory protein HypB)|interpro:IPR027417	-	-	-	figure legend:Figure 1B|comment:"HycE co-eluted with HypAStr in the unprocessed form (supplemental Fig. S1)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:bg58XWiAv28fGfbHi898yF5HN8o83333	intact-crc:46D210951C25954B|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0A700	uniprotkb:P0A9K9	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-369251|uniprotkb:P30856|uniprotkb:Q2M714|ensemblbacteria:AAC76374|ensemblbacteria:BAE77942	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:slyd_ecoli(display_long)|uniprotkb:slyD(gene name)|psi-mi:slyD(display_short)|uniprotkb:b3349(locus name)|uniprotkb:Metallochaperone SlyD(gene name synonym)|uniprotkb:JW3311(locus name)|uniprotkb:Histidine-rich protein(gene name synonym)|uniprotkb:WHP(gene name synonym)|uniprotkb:Rotamase(gene name synonym)|uniprotkb:Sensitivity to lysis protein D(gene name synonym)	psi-mi:"MI:0096"(pull down)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6290903|imex:IM-17822-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417808.1|refseq:WP_000861334.1|ensemblbacteria:AAC76374(transcript)|ensemblbacteria:b3349(gene)|ensemblbacteria:BAE77942(gene)|ensemblbacteria:BAE77942(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009408"(response to heat)|go:"GO:0016151"(nickel cation binding)|go:"GO:0022417"(protein maturation by protein folding)|go:"GO:0042026"(protein refolding)|go:"GO:0043963"(modulation by symbiont of host adenylate cyclase-mediated signal transduction)|go:"GO:0050821"(protein stabilization)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR001179(Peptidyl-prolyl cis-trans isomerase, FKBP-type)|rcsb pdb:2K8I|rcsb pdb:2KFW|rcsb pdb:5I7P|dip:DIP-31853N	-	-	-	figure legend:Figure 1B|comment:"HycE co-eluted with HypAStr in the unprocessed form (supplemental Fig. S1)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:Y/b//pPxL6aoUOf5NH2HKiB+wN883333	intact-crc:46D210951C25954B|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P16431	uniprotkb:P0A700	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:"MI:0096"(pull down)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6291006|imex:IM-17822-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	-	-	-	figure legend:supplemental Fig. S2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	intact-crc:9D4465479A75FEB7|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:n-n	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P16431	uniprotkb:P0A9K9	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-369251|uniprotkb:P30856|uniprotkb:Q2M714|ensemblbacteria:AAC76374|ensemblbacteria:BAE77942	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:slyd_ecoli(display_long)|uniprotkb:slyD(gene name)|psi-mi:slyD(display_short)|uniprotkb:b3349(locus name)|uniprotkb:Metallochaperone SlyD(gene name synonym)|uniprotkb:JW3311(locus name)|uniprotkb:Histidine-rich protein(gene name synonym)|uniprotkb:WHP(gene name synonym)|uniprotkb:Rotamase(gene name synonym)|uniprotkb:Sensitivity to lysis protein D(gene name synonym)	psi-mi:"MI:0096"(pull down)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6291006|imex:IM-17822-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	refseq:NP_417808.1|refseq:WP_000861334.1|ensemblbacteria:AAC76374(transcript)|ensemblbacteria:b3349(gene)|ensemblbacteria:BAE77942(gene)|ensemblbacteria:BAE77942(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009408"(response to heat)|go:"GO:0016151"(nickel cation binding)|go:"GO:0022417"(protein maturation by protein folding)|go:"GO:0042026"(protein refolding)|go:"GO:0043963"(modulation by symbiont of host adenylate cyclase-mediated signal transduction)|go:"GO:0050821"(protein stabilization)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR001179(Peptidyl-prolyl cis-trans isomerase, FKBP-type)|rcsb pdb:2K8I|rcsb pdb:2KFW|rcsb pdb:5I7P|dip:DIP-31853N	-	-	-	figure legend:supplemental Fig. S2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:Y/b//pPxL6aoUOf5NH2HKiB+wN883333	intact-crc:9D4465479A75FEB7|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:n-n	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P16431	uniprotkb:P0AAN3	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-558261|uniprotkb:P24190|uniprotkb:Q2MAA2|uniprotkb:Q46884|ensemblbacteria:AAC75769|ensemblbacteria:BAE76804	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:hypb_ecoli(display_long)|uniprotkb:hypB(gene name)|psi-mi:hypB(display_short)|uniprotkb:Hydrogenase isoenzymes nickel incorporation protein HypB(gene name synonym)|uniprotkb:JW2697(locus name)|uniprotkb:b2727(locus name)	psi-mi:"MI:0096"(pull down)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6291006|imex:IM-17822-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	refseq:NP_417207.1|refseq:WP_000337665.1|dip:DIP-36428N|ensemblbacteria:AAC75769(transcript)|ensemblbacteria:b2727(gene)|ensemblbacteria:BAE76804(gene)|ensemblbacteria:BAE76804(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0051604"(protein maturation)|interpro:IPR003495("Cobalamin (vitamin B12) biosynthesis CobW-like")|interpro:IPR004392(Hydrogenase accessory protein HypB)|interpro:IPR027417	-	-	-	figure legend:supplemental Fig. S2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:bg58XWiAv28fGfbHi898yF5HN8o83333	intact-crc:9D4465479A75FEB7|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:n-n	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P16431	uniprotkb:P0A700	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6291171|imex:IM-17822-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	-	-	-	figure legend:Figure 2A, Supplemental data Figure S2|comment:"The use of cross-linker resulted in detection of increased amounts of co-eluting nickel insertion proteins with HycEStr (supplemental Fig. S2)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	intact-crc:37C9F969275232D0|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:n-n	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P16431	uniprotkb:P0A9K9	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-369251|uniprotkb:P30856|uniprotkb:Q2M714|ensemblbacteria:AAC76374|ensemblbacteria:BAE77942	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:slyd_ecoli(display_long)|uniprotkb:slyD(gene name)|psi-mi:slyD(display_short)|uniprotkb:b3349(locus name)|uniprotkb:Metallochaperone SlyD(gene name synonym)|uniprotkb:JW3311(locus name)|uniprotkb:Histidine-rich protein(gene name synonym)|uniprotkb:WHP(gene name synonym)|uniprotkb:Rotamase(gene name synonym)|uniprotkb:Sensitivity to lysis protein D(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6291171|imex:IM-17822-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	refseq:NP_417808.1|refseq:WP_000861334.1|ensemblbacteria:AAC76374(transcript)|ensemblbacteria:b3349(gene)|ensemblbacteria:BAE77942(gene)|ensemblbacteria:BAE77942(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009408"(response to heat)|go:"GO:0016151"(nickel cation binding)|go:"GO:0022417"(protein maturation by protein folding)|go:"GO:0042026"(protein refolding)|go:"GO:0043963"(modulation by symbiont of host adenylate cyclase-mediated signal transduction)|go:"GO:0050821"(protein stabilization)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR001179(Peptidyl-prolyl cis-trans isomerase, FKBP-type)|rcsb pdb:2K8I|rcsb pdb:2KFW|rcsb pdb:5I7P|dip:DIP-31853N	-	-	-	figure legend:Figure 2A, Supplemental data Figure S2|comment:"The use of cross-linker resulted in detection of increased amounts of co-eluting nickel insertion proteins with HycEStr (supplemental Fig. S2)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:Y/b//pPxL6aoUOf5NH2HKiB+wN883333	intact-crc:37C9F969275232D0|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:n-n	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P16431	uniprotkb:P0AAN3	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-558261|uniprotkb:P24190|uniprotkb:Q2MAA2|uniprotkb:Q46884|ensemblbacteria:AAC75769|ensemblbacteria:BAE76804	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:hypb_ecoli(display_long)|uniprotkb:hypB(gene name)|psi-mi:hypB(display_short)|uniprotkb:Hydrogenase isoenzymes nickel incorporation protein HypB(gene name synonym)|uniprotkb:JW2697(locus name)|uniprotkb:b2727(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6291171|imex:IM-17822-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	refseq:NP_417207.1|refseq:WP_000337665.1|dip:DIP-36428N|ensemblbacteria:AAC75769(transcript)|ensemblbacteria:b2727(gene)|ensemblbacteria:BAE76804(gene)|ensemblbacteria:BAE76804(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0051604"(protein maturation)|interpro:IPR003495("Cobalamin (vitamin B12) biosynthesis CobW-like")|interpro:IPR004392(Hydrogenase accessory protein HypB)|interpro:IPR027417	-	-	-	figure legend:Figure 2A, Supplemental data Figure S2|comment:"The use of cross-linker resulted in detection of increased amounts of co-eluting nickel insertion proteins with HycEStr (supplemental Fig. S2)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:bg58XWiAv28fGfbHi898yF5HN8o83333	intact-crc:37C9F969275232D0|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:n-n	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0A700	uniprotkb:P0A9K9	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-369251|uniprotkb:P30856|uniprotkb:Q2M714|ensemblbacteria:AAC76374|ensemblbacteria:BAE77942	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:slyd_ecoli(display_long)|uniprotkb:slyD(gene name)|psi-mi:slyD(display_short)|uniprotkb:b3349(locus name)|uniprotkb:Metallochaperone SlyD(gene name synonym)|uniprotkb:JW3311(locus name)|uniprotkb:Histidine-rich protein(gene name synonym)|uniprotkb:WHP(gene name synonym)|uniprotkb:Rotamase(gene name synonym)|uniprotkb:Sensitivity to lysis protein D(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6291213|imex:IM-17822-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417808.1|refseq:WP_000861334.1|ensemblbacteria:AAC76374(transcript)|ensemblbacteria:b3349(gene)|ensemblbacteria:BAE77942(gene)|ensemblbacteria:BAE77942(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009408"(response to heat)|go:"GO:0016151"(nickel cation binding)|go:"GO:0022417"(protein maturation by protein folding)|go:"GO:0042026"(protein refolding)|go:"GO:0043963"(modulation by symbiont of host adenylate cyclase-mediated signal transduction)|go:"GO:0050821"(protein stabilization)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR001179(Peptidyl-prolyl cis-trans isomerase, FKBP-type)|rcsb pdb:2K8I|rcsb pdb:2KFW|rcsb pdb:5I7P|dip:DIP-31853N	-	-	-	figure legend:Figure 2B|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:Y/b//pPxL6aoUOf5NH2HKiB+wN883333	intact-crc:77A66D469F128744|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep ii tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0A700	uniprotkb:P0AAN3	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-558261|uniprotkb:P24190|uniprotkb:Q2MAA2|uniprotkb:Q46884|ensemblbacteria:AAC75769|ensemblbacteria:BAE76804	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:hypb_ecoli(display_long)|uniprotkb:hypB(gene name)|psi-mi:hypB(display_short)|uniprotkb:Hydrogenase isoenzymes nickel incorporation protein HypB(gene name synonym)|uniprotkb:JW2697(locus name)|uniprotkb:b2727(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6291213|imex:IM-17822-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417207.1|refseq:WP_000337665.1|dip:DIP-36428N|ensemblbacteria:AAC75769(transcript)|ensemblbacteria:b2727(gene)|ensemblbacteria:BAE76804(gene)|ensemblbacteria:BAE76804(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0051604"(protein maturation)|interpro:IPR003495("Cobalamin (vitamin B12) biosynthesis CobW-like")|interpro:IPR004392(Hydrogenase accessory protein HypB)|interpro:IPR027417	-	-	-	figure legend:Figure 2B|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:bg58XWiAv28fGfbHi898yF5HN8o83333	intact-crc:77A66D469F128744|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep ii tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0A700	uniprotkb:P16431	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6291213|imex:IM-17822-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	-	-	-	figure legend:Figure 2B|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	intact-crc:77A66D469F128744|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep ii tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0A700	uniprotkb:P16431	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6292798|imex:IM-17822-8	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	-	-	-	figure legend:Figure 3A|comment:"Pulldown assays with HycEStr were performed in DHP-B strain (no expression of hypB) and delta slyD strain (no expression of slyD)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	intact-crc:3073F51769B4FF05|rigid:w/uLtv/jyi9hxWKb324ZPM/HYYE	false	-	strep tag:n-n	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P16431	uniprotkb:P0A700	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6292857|imex:IM-17822-9	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	-	-	-	figure legend:Figure 3 B|comment:"Pulldown assays with HypAStr were performed in DHP-B strain (no expression of hypB) and delta slyD strain (no expression of slyD)"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	intact-crc:D367458D98D82AB7|rigid:w/uLtv/jyi9hxWKb324ZPM/HYYE	false	-	strep tag:c-c	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0A700	uniprotkb:P0A9K9	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-369251|uniprotkb:P30856|uniprotkb:Q2M714|ensemblbacteria:AAC76374|ensemblbacteria:BAE77942	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:slyd_ecoli(display_long)|uniprotkb:slyD(gene name)|psi-mi:slyD(display_short)|uniprotkb:b3349(locus name)|uniprotkb:Metallochaperone SlyD(gene name synonym)|uniprotkb:JW3311(locus name)|uniprotkb:Histidine-rich protein(gene name synonym)|uniprotkb:WHP(gene name synonym)|uniprotkb:Rotamase(gene name synonym)|uniprotkb:Sensitivity to lysis protein D(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303114|imex:IM-17822-11	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417808.1|refseq:WP_000861334.1|ensemblbacteria:AAC76374(transcript)|ensemblbacteria:b3349(gene)|ensemblbacteria:BAE77942(gene)|ensemblbacteria:BAE77942(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009408"(response to heat)|go:"GO:0016151"(nickel cation binding)|go:"GO:0022417"(protein maturation by protein folding)|go:"GO:0042026"(protein refolding)|go:"GO:0043963"(modulation by symbiont of host adenylate cyclase-mediated signal transduction)|go:"GO:0050821"(protein stabilization)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR001179(Peptidyl-prolyl cis-trans isomerase, FKBP-type)|rcsb pdb:2K8I|rcsb pdb:2KFW|rcsb pdb:5I7P|dip:DIP-31853N	-	-	-	figure legend:Figure 3C|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:Y/b//pPxL6aoUOf5NH2HKiB+wN883333	intact-crc:55E97945FBD222A7|rigid:Ng5UdNpogoNYl20usvh0YKNdVHg	false	strep tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0A700	uniprotkb:P0AAN3	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-558261|uniprotkb:P24190|uniprotkb:Q2MAA2|uniprotkb:Q46884|ensemblbacteria:AAC75769|ensemblbacteria:BAE76804	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:hypb_ecoli(display_long)|uniprotkb:hypB(gene name)|psi-mi:hypB(display_short)|uniprotkb:Hydrogenase isoenzymes nickel incorporation protein HypB(gene name synonym)|uniprotkb:JW2697(locus name)|uniprotkb:b2727(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303114|imex:IM-17822-11	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417207.1|refseq:WP_000337665.1|dip:DIP-36428N|ensemblbacteria:AAC75769(transcript)|ensemblbacteria:b2727(gene)|ensemblbacteria:BAE76804(gene)|ensemblbacteria:BAE76804(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0051604"(protein maturation)|interpro:IPR003495("Cobalamin (vitamin B12) biosynthesis CobW-like")|interpro:IPR004392(Hydrogenase accessory protein HypB)|interpro:IPR027417	-	-	-	figure legend:Figure 3C|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:bg58XWiAv28fGfbHi898yF5HN8o83333	intact-crc:55E97945FBD222A7|rigid:Ng5UdNpogoNYl20usvh0YKNdVHg	false	strep tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P16431	uniprotkb:P0A700	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303179|imex:IM-17822-13	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	-	-	-	figure legend:Figure 5C|comment:"The pulldown assay was performed in the bacterial strain HYD723 (delta nikA), which lacks the soluble  periplasmic component of the NikABCDE transporter"|comment:A marked decrease in the amount of HypA pulled down was observed in HYD723 compared with the MC4100 wild-type strain|comment:"When extra nickel was added to the growth media of the HYD723 cells only a very slight increase in the amount of HypA pulled down with HycEStr was observed (Fig. 5C)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	intact-crc:1E1243AAF4931839|rigid:w/uLtv/jyi9hxWKb324ZPM/HYYE	false	strep tag:n-n	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0A700	uniprotkb:P16431	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303187|imex:IM-17822-14	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	-	-	-	figure legend:supplemental data Figure S6|comment:The amount of HypB and SlyD pulled down with HypAStr from HYD723 cell lysates was not dramatically affected|comment:"The pulldown assay was performed in the bacterial strain HYD723 (delta nikA), which lacks the soluble periplasmic component of the NikABCDE transporter"|comment:A marked decrease in the amount of HycE pulled down was observed in HYD723 compared with the MC4100 wild-type strain|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	intact-crc:3D45CB36CF260079|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0A700	uniprotkb:P0AAN3	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-558261|uniprotkb:P24190|uniprotkb:Q2MAA2|uniprotkb:Q46884|ensemblbacteria:AAC75769|ensemblbacteria:BAE76804	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:hypb_ecoli(display_long)|uniprotkb:hypB(gene name)|psi-mi:hypB(display_short)|uniprotkb:Hydrogenase isoenzymes nickel incorporation protein HypB(gene name synonym)|uniprotkb:JW2697(locus name)|uniprotkb:b2727(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303187|imex:IM-17822-14	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417207.1|refseq:WP_000337665.1|dip:DIP-36428N|ensemblbacteria:AAC75769(transcript)|ensemblbacteria:b2727(gene)|ensemblbacteria:BAE76804(gene)|ensemblbacteria:BAE76804(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0051604"(protein maturation)|interpro:IPR003495("Cobalamin (vitamin B12) biosynthesis CobW-like")|interpro:IPR004392(Hydrogenase accessory protein HypB)|interpro:IPR027417	-	-	-	figure legend:supplemental data Figure S6|comment:The amount of HypB and SlyD pulled down with HypAStr from HYD723 cell lysates was not dramatically affected|comment:"The pulldown assay was performed in the bacterial strain HYD723 (delta nikA), which lacks the soluble periplasmic component of the NikABCDE transporter"|comment:A marked decrease in the amount of HycE pulled down was observed in HYD723 compared with the MC4100 wild-type strain|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:bg58XWiAv28fGfbHi898yF5HN8o83333	intact-crc:3D45CB36CF260079|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0A700	uniprotkb:P0A9K9	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-369251|uniprotkb:P30856|uniprotkb:Q2M714|ensemblbacteria:AAC76374|ensemblbacteria:BAE77942	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:slyd_ecoli(display_long)|uniprotkb:slyD(gene name)|psi-mi:slyD(display_short)|uniprotkb:b3349(locus name)|uniprotkb:Metallochaperone SlyD(gene name synonym)|uniprotkb:JW3311(locus name)|uniprotkb:Histidine-rich protein(gene name synonym)|uniprotkb:WHP(gene name synonym)|uniprotkb:Rotamase(gene name synonym)|uniprotkb:Sensitivity to lysis protein D(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303187|imex:IM-17822-14	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417808.1|refseq:WP_000861334.1|ensemblbacteria:AAC76374(transcript)|ensemblbacteria:b3349(gene)|ensemblbacteria:BAE77942(gene)|ensemblbacteria:BAE77942(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009408"(response to heat)|go:"GO:0016151"(nickel cation binding)|go:"GO:0022417"(protein maturation by protein folding)|go:"GO:0042026"(protein refolding)|go:"GO:0043963"(modulation by symbiont of host adenylate cyclase-mediated signal transduction)|go:"GO:0050821"(protein stabilization)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR001179(Peptidyl-prolyl cis-trans isomerase, FKBP-type)|rcsb pdb:2K8I|rcsb pdb:2KFW|rcsb pdb:5I7P|dip:DIP-31853N	-	-	-	figure legend:supplemental data Figure S6|comment:The amount of HypB and SlyD pulled down with HypAStr from HYD723 cell lysates was not dramatically affected|comment:"The pulldown assay was performed in the bacterial strain HYD723 (delta nikA), which lacks the soluble periplasmic component of the NikABCDE transporter"|comment:A marked decrease in the amount of HycE pulled down was observed in HYD723 compared with the MC4100 wild-type strain|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:Y/b//pPxL6aoUOf5NH2HKiB+wN883333	intact-crc:3D45CB36CF260079|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P16431	uniprotkb:P0A700	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303235|imex:IM-17822-15	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	-	-	-	figure legend:supplemental data Figure S7|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	intact-crc:4ABC9183772FE92E|rigid:w/uLtv/jyi9hxWKb324ZPM/HYYE	false	strep tag:n-n	mutation:2-2	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0A700	uniprotkb:P0AAN3	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-558261|uniprotkb:P24190|uniprotkb:Q2MAA2|uniprotkb:Q46884|ensemblbacteria:AAC75769|ensemblbacteria:BAE76804	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:hypb_ecoli(display_long)|uniprotkb:hypB(gene name)|psi-mi:hypB(display_short)|uniprotkb:Hydrogenase isoenzymes nickel incorporation protein HypB(gene name synonym)|uniprotkb:JW2697(locus name)|uniprotkb:b2727(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6291311|imex:IM-17822-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417207.1|refseq:WP_000337665.1|dip:DIP-36428N|ensemblbacteria:AAC75769(transcript)|ensemblbacteria:b2727(gene)|ensemblbacteria:BAE76804(gene)|ensemblbacteria:BAE76804(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0051604"(protein maturation)|interpro:IPR003495("Cobalamin (vitamin B12) biosynthesis CobW-like")|interpro:IPR004392(Hydrogenase accessory protein HypB)|interpro:IPR027417	-	-	-	figure legend:supplemental data Table S1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:bg58XWiAv28fGfbHi898yF5HN8o83333	intact-crc:F3DB3EFEFBB41C02|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:c-c	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P0A700	uniprotkb:P16431	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6291311|imex:IM-17822-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	-	-	-	figure legend:supplemental data Table S1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	intact-crc:F3DB3EFEFBB41C02|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:c-c	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P0A700	uniprotkb:P0A9K9	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-369251|uniprotkb:P30856|uniprotkb:Q2M714|ensemblbacteria:AAC76374|ensemblbacteria:BAE77942	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:slyd_ecoli(display_long)|uniprotkb:slyD(gene name)|psi-mi:slyD(display_short)|uniprotkb:b3349(locus name)|uniprotkb:Metallochaperone SlyD(gene name synonym)|uniprotkb:JW3311(locus name)|uniprotkb:Histidine-rich protein(gene name synonym)|uniprotkb:WHP(gene name synonym)|uniprotkb:Rotamase(gene name synonym)|uniprotkb:Sensitivity to lysis protein D(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6291311|imex:IM-17822-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417808.1|refseq:WP_000861334.1|ensemblbacteria:AAC76374(transcript)|ensemblbacteria:b3349(gene)|ensemblbacteria:BAE77942(gene)|ensemblbacteria:BAE77942(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009408"(response to heat)|go:"GO:0016151"(nickel cation binding)|go:"GO:0022417"(protein maturation by protein folding)|go:"GO:0042026"(protein refolding)|go:"GO:0043963"(modulation by symbiont of host adenylate cyclase-mediated signal transduction)|go:"GO:0050821"(protein stabilization)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR001179(Peptidyl-prolyl cis-trans isomerase, FKBP-type)|rcsb pdb:2K8I|rcsb pdb:2KFW|rcsb pdb:5I7P|dip:DIP-31853N	-	-	-	figure legend:supplemental data Table S1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:Y/b//pPxL6aoUOf5NH2HKiB+wN883333	intact-crc:F3DB3EFEFBB41C02|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:c-c	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P16431	uniprotkb:P0A700	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303087|imex:IM-17822-10	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	-	-	-	figure legend:supplemental data Table S1|comment:"when HycEStr pulldown experiments were performed from the DPABF strain, HypB could not be detected by Western blot (data not shown) or by LC-MS/MS (supplemental Table S1), supporting a role for HypA in mediating complex formation between HypB and HycE."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	intact-crc:2929721417E65A93|rigid:w/uLtv/jyi9hxWKb324ZPM/HYYE	false	-	strep tag:c-c	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P0A700	uniprotkb:P0A9K9	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-369251|uniprotkb:P30856|uniprotkb:Q2M714|ensemblbacteria:AAC76374|ensemblbacteria:BAE77942	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:slyd_ecoli(display_long)|uniprotkb:slyD(gene name)|psi-mi:slyD(display_short)|uniprotkb:b3349(locus name)|uniprotkb:Metallochaperone SlyD(gene name synonym)|uniprotkb:JW3311(locus name)|uniprotkb:Histidine-rich protein(gene name synonym)|uniprotkb:WHP(gene name synonym)|uniprotkb:Rotamase(gene name synonym)|uniprotkb:Sensitivity to lysis protein D(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303150|imex:IM-17822-12	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417808.1|refseq:WP_000861334.1|ensemblbacteria:AAC76374(transcript)|ensemblbacteria:b3349(gene)|ensemblbacteria:BAE77942(gene)|ensemblbacteria:BAE77942(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009408"(response to heat)|go:"GO:0016151"(nickel cation binding)|go:"GO:0022417"(protein maturation by protein folding)|go:"GO:0042026"(protein refolding)|go:"GO:0043963"(modulation by symbiont of host adenylate cyclase-mediated signal transduction)|go:"GO:0050821"(protein stabilization)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR001179(Peptidyl-prolyl cis-trans isomerase, FKBP-type)|rcsb pdb:2K8I|rcsb pdb:2KFW|rcsb pdb:5I7P|dip:DIP-31853N	-	-	-	figure legend:Supplemental data Table S2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:Y/b//pPxL6aoUOf5NH2HKiB+wN883333	intact-crc:B09340D71901979E|rigid:Ng5UdNpogoNYl20usvh0YKNdVHg	false	strep tag:c-c	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P0A700	uniprotkb:P0AAN3	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-558261|uniprotkb:P24190|uniprotkb:Q2MAA2|uniprotkb:Q46884|ensemblbacteria:AAC75769|ensemblbacteria:BAE76804	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:hypb_ecoli(display_long)|uniprotkb:hypB(gene name)|psi-mi:hypB(display_short)|uniprotkb:Hydrogenase isoenzymes nickel incorporation protein HypB(gene name synonym)|uniprotkb:JW2697(locus name)|uniprotkb:b2727(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303150|imex:IM-17822-12	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417207.1|refseq:WP_000337665.1|dip:DIP-36428N|ensemblbacteria:AAC75769(transcript)|ensemblbacteria:b2727(gene)|ensemblbacteria:BAE76804(gene)|ensemblbacteria:BAE76804(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0051604"(protein maturation)|interpro:IPR003495("Cobalamin (vitamin B12) biosynthesis CobW-like")|interpro:IPR004392(Hydrogenase accessory protein HypB)|interpro:IPR027417	-	-	-	figure legend:Supplemental data Table S2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:bg58XWiAv28fGfbHi898yF5HN8o83333	intact-crc:B09340D71901979E|rigid:Ng5UdNpogoNYl20usvh0YKNdVHg	false	strep tag:c-c	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P16431	uniprotkb:P0A9K9	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-369251|uniprotkb:P30856|uniprotkb:Q2M714|ensemblbacteria:AAC76374|ensemblbacteria:BAE77942	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:slyd_ecoli(display_long)|uniprotkb:slyD(gene name)|psi-mi:slyD(display_short)|uniprotkb:b3349(locus name)|uniprotkb:Metallochaperone SlyD(gene name synonym)|uniprotkb:JW3311(locus name)|uniprotkb:Histidine-rich protein(gene name synonym)|uniprotkb:WHP(gene name synonym)|uniprotkb:Rotamase(gene name synonym)|uniprotkb:Sensitivity to lysis protein D(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6305715|imex:IM-17822-18	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	refseq:NP_417808.1|refseq:WP_000861334.1|ensemblbacteria:AAC76374(transcript)|ensemblbacteria:b3349(gene)|ensemblbacteria:BAE77942(gene)|ensemblbacteria:BAE77942(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009408"(response to heat)|go:"GO:0016151"(nickel cation binding)|go:"GO:0022417"(protein maturation by protein folding)|go:"GO:0042026"(protein refolding)|go:"GO:0043963"(modulation by symbiont of host adenylate cyclase-mediated signal transduction)|go:"GO:0050821"(protein stabilization)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR001179(Peptidyl-prolyl cis-trans isomerase, FKBP-type)|rcsb pdb:2K8I|rcsb pdb:2KFW|rcsb pdb:5I7P|dip:DIP-31853N	-	-	-	figure legend:Supplemental data Table S2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:Y/b//pPxL6aoUOf5NH2HKiB+wN883333	intact-crc:3DA6D543AEFBC7EF|rigid:Q9sT1Cue26yPtzHUFcesQWH/fZo	false	strep tag:n-n	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P16431	uniprotkb:P0A6F5	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-543750|ensemblbacteria:AAC77103|ensemblbacteria:BAE78145|uniprotkb:P06139|uniprotkb:Q2M6G1	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:ch60_ecoli(display_long)|uniprotkb:groEL(gene name)|psi-mi:groEL(display_short)|uniprotkb:60 kDa chaperonin(gene name synonym)|uniprotkb:Chaperonin-60(gene name synonym)|uniprotkb:GroEL protein(gene name synonym)|uniprotkb:groL(gene name synonym)|uniprotkb:mopA(gene name synonym)|uniprotkb:JW4103(locus name)|uniprotkb:b4143(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6305715|imex:IM-17822-18	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	ensemblbacteria:AAC77103(transcript)|ensemblbacteria:b4143(gene)|ensemblbacteria:BAE78145(gene)|ensemblbacteria:BAE78145(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0006457"(protein folding)|go:"GO:0007049"(cell cycle)|go:"GO:0009314"(response to radiation)|go:"GO:0009408"(response to heat)|go:"GO:0016020"(membrane)|go:"GO:0019068"(virion assembly)|go:"GO:0042026"(protein refolding)|go:"GO:0042802"(identical protein binding)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051085"(chaperone cofactor-dependent protein refolding)|go:"GO:0051301"(cell division)|go:"GO:1990220"(GroEL-GroES complex)|interpro:IPR001844(Chaperonin Cpn60)|interpro:IPR002423(Chaperonin Cpn60/TCP-1)|interpro:IPR018370|interpro:IPR027409|interpro:IPR027410|interpro:IPR027413|rcsb pdb:1AON|rcsb pdb:1DK7|rcsb pdb:1DKD|rcsb pdb:1FY9|rcsb pdb:1FYA|rcsb pdb:1GR5|rcsb pdb:1GRL|rcsb pdb:1GRU|rcsb pdb:1JON|rcsb pdb:1KID|rcsb pdb:1KP8|rcsb pdb:1LA1|rcsb pdb:1MNF|rcsb pdb:1OEL|rcsb pdb:1PCQ|rcsb pdb:1PF9|rcsb pdb:1SS8|rcsb pdb:1SVT|rcsb pdb:1SX3|rcsb pdb:1SX4|rcsb pdb:1XCK|rcsb pdb:2C7C|rcsb pdb:2C7D|rcsb pdb:2C7E|rcsb pdb:2CGT|rcsb pdb:2EU1|rcsb pdb:2NWC|rcsb pdb:2YEY|refseq:NP_418567.1|refseq:WP_000729117.1|rcsb pdb:3CAU|rcsb pdb:3VZ6|rcsb pdb:3VZ7|rcsb pdb:3VZ8|rcsb pdb:3WVL|rcsb pdb:3ZPZ|rcsb pdb:3ZQ0|rcsb pdb:3ZQ1|rcsb pdb:4AAQ|rcsb pdb:4AAR|rcsb pdb:4AAS|rcsb pdb:4AAU|rcsb pdb:4AB2|rcsb pdb:4AB3|rcsb pdb:4V43|rcsb pdb:4WGL|rcsb pdb:4WSC|rcsb pdb:5OPW|rcsb pdb:5OPX|rcsb pdb:5W0S|rcsb pdb:3C9V|mint:P0A6F5|dip:DIP-339N	-	-	crc64:CD3A0FB505F74AD1	figure legend:Supplemental data Table S2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:TuNoPwyx7V5IaOQVjtdcc6sctqw83333	intact-crc:3DA6D543AEFBC7EF|rigid:Q9sT1Cue26yPtzHUFcesQWH/fZo	false	strep tag:n-n	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P0A700	uniprotkb:P16431	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6290753|imex:IM-17822-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	-	-	-	figure legend:supplemental Fig. S3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	intact-crc:87CC7B3E2E603418|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0A700	uniprotkb:P0A9K9	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-369251|uniprotkb:P30856|uniprotkb:Q2M714|ensemblbacteria:AAC76374|ensemblbacteria:BAE77942	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:slyd_ecoli(display_long)|uniprotkb:slyD(gene name)|psi-mi:slyD(display_short)|uniprotkb:b3349(locus name)|uniprotkb:Metallochaperone SlyD(gene name synonym)|uniprotkb:JW3311(locus name)|uniprotkb:Histidine-rich protein(gene name synonym)|uniprotkb:WHP(gene name synonym)|uniprotkb:Rotamase(gene name synonym)|uniprotkb:Sensitivity to lysis protein D(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6290753|imex:IM-17822-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417808.1|refseq:WP_000861334.1|ensemblbacteria:AAC76374(transcript)|ensemblbacteria:b3349(gene)|ensemblbacteria:BAE77942(gene)|ensemblbacteria:BAE77942(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009408"(response to heat)|go:"GO:0016151"(nickel cation binding)|go:"GO:0022417"(protein maturation by protein folding)|go:"GO:0042026"(protein refolding)|go:"GO:0043963"(modulation by symbiont of host adenylate cyclase-mediated signal transduction)|go:"GO:0050821"(protein stabilization)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR001179(Peptidyl-prolyl cis-trans isomerase, FKBP-type)|rcsb pdb:2K8I|rcsb pdb:2KFW|rcsb pdb:5I7P|dip:DIP-31853N	-	-	-	figure legend:supplemental Fig. S3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:Y/b//pPxL6aoUOf5NH2HKiB+wN883333	intact-crc:87CC7B3E2E603418|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0A700	uniprotkb:P0AAN3	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-558261|uniprotkb:P24190|uniprotkb:Q2MAA2|uniprotkb:Q46884|ensemblbacteria:AAC75769|ensemblbacteria:BAE76804	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:hypb_ecoli(display_long)|uniprotkb:hypB(gene name)|psi-mi:hypB(display_short)|uniprotkb:Hydrogenase isoenzymes nickel incorporation protein HypB(gene name synonym)|uniprotkb:JW2697(locus name)|uniprotkb:b2727(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6290753|imex:IM-17822-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417207.1|refseq:WP_000337665.1|dip:DIP-36428N|ensemblbacteria:AAC75769(transcript)|ensemblbacteria:b2727(gene)|ensemblbacteria:BAE76804(gene)|ensemblbacteria:BAE76804(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0051604"(protein maturation)|interpro:IPR003495("Cobalamin (vitamin B12) biosynthesis CobW-like")|interpro:IPR004392(Hydrogenase accessory protein HypB)|interpro:IPR027417	-	-	-	figure legend:supplemental Fig. S3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:bg58XWiAv28fGfbHi898yF5HN8o83333	intact-crc:87CC7B3E2E603418|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P16431	uniprotkb:P0A700	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6290801|imex:IM-17822-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	-	-	-	figure legend:supplemental Fig. S3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	intact-crc:DB43032735414ADA|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:n-n	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P16431	uniprotkb:P0A9K9	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-369251|uniprotkb:P30856|uniprotkb:Q2M714|ensemblbacteria:AAC76374|ensemblbacteria:BAE77942	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:slyd_ecoli(display_long)|uniprotkb:slyD(gene name)|psi-mi:slyD(display_short)|uniprotkb:b3349(locus name)|uniprotkb:Metallochaperone SlyD(gene name synonym)|uniprotkb:JW3311(locus name)|uniprotkb:Histidine-rich protein(gene name synonym)|uniprotkb:WHP(gene name synonym)|uniprotkb:Rotamase(gene name synonym)|uniprotkb:Sensitivity to lysis protein D(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6290801|imex:IM-17822-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	refseq:NP_417808.1|refseq:WP_000861334.1|ensemblbacteria:AAC76374(transcript)|ensemblbacteria:b3349(gene)|ensemblbacteria:BAE77942(gene)|ensemblbacteria:BAE77942(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009408"(response to heat)|go:"GO:0016151"(nickel cation binding)|go:"GO:0022417"(protein maturation by protein folding)|go:"GO:0042026"(protein refolding)|go:"GO:0043963"(modulation by symbiont of host adenylate cyclase-mediated signal transduction)|go:"GO:0050821"(protein stabilization)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR001179(Peptidyl-prolyl cis-trans isomerase, FKBP-type)|rcsb pdb:2K8I|rcsb pdb:2KFW|rcsb pdb:5I7P|dip:DIP-31853N	-	-	-	figure legend:supplemental Fig. S3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:Y/b//pPxL6aoUOf5NH2HKiB+wN883333	intact-crc:DB43032735414ADA|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:n-n	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P16431	uniprotkb:P0AAN3	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-558261|uniprotkb:P24190|uniprotkb:Q2MAA2|uniprotkb:Q46884|ensemblbacteria:AAC75769|ensemblbacteria:BAE76804	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:hypb_ecoli(display_long)|uniprotkb:hypB(gene name)|psi-mi:hypB(display_short)|uniprotkb:Hydrogenase isoenzymes nickel incorporation protein HypB(gene name synonym)|uniprotkb:JW2697(locus name)|uniprotkb:b2727(locus name)	psi-mi:"MI:0030"(cross-linking study)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6290801|imex:IM-17822-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	refseq:NP_417207.1|refseq:WP_000337665.1|dip:DIP-36428N|ensemblbacteria:AAC75769(transcript)|ensemblbacteria:b2727(gene)|ensemblbacteria:BAE76804(gene)|ensemblbacteria:BAE76804(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0051604"(protein maturation)|interpro:IPR003495("Cobalamin (vitamin B12) biosynthesis CobW-like")|interpro:IPR004392(Hydrogenase accessory protein HypB)|interpro:IPR027417	-	-	-	figure legend:supplemental Fig. S3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:bg58XWiAv28fGfbHi898yF5HN8o83333	intact-crc:DB43032735414ADA|rigid:KG4c2ybPMrhcWmSTBdDOGtT3xYk	false	strep tag:n-n	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0A700	uniprotkb:P0A9Q7	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-543417|uniprotkb:P17547|ensemblbacteria:AAC74323|ensemblbacteria:BAA36121	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:adhe_ecoli(display_long)|uniprotkb:adhE(gene name)|psi-mi:adhE(display_short)|uniprotkb:ana(gene name synonym)|uniprotkb:b1241(locus name)|uniprotkb:JW1228(locus name)	psi-mi:"MI:0096"(pull down)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303306|imex:IM-17822-16	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_415757.1|refseq:WP_000301651.1|ensemblbacteria:AAC74323(transcript)|ensemblbacteria:b1241(gene)|ensemblbacteria:BAA36121(transcript)|go:"GO:0004022"("alcohol dehydrogenase (NAD+) activity")|go:"GO:0005829"(cytosol)|go:"GO:0006115"(ethanol biosynthetic process)|go:"GO:0008774"("acetaldehyde dehydrogenase (acetylating) activity")|go:"GO:0015976"(carbon utilization)|go:"GO:0016020"(membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0046872"(metal ion binding)|interpro:IPR001670(Iron-containing alcohol dehydrogenase)|interpro:IPR012079(Bifunctional aldehyde/alcohol dehydrogenase)|interpro:IPR015590(Aldehyde dehydrogenase)|interpro:IPR016161(Aldehyde/histidinol dehydrogenase)|interpro:IPR016162(Aldehyde dehydrogenase, N-terminal)|interpro:IPR016163(Aldehyde dehydrogenase, C-terminal)|interpro:IPR018211|interpro:IPR034789|interpro:IPR039697|rcsb pdb:6AHC|rcsb pdb:6SCG|rcsb pdb:6SCI|rcsb pdb:6TQH|rcsb pdb:6TQM|rcsb pdb:7BVP|ensemblbacteria:BAA36121(gene)|mint:P0A9Q7|dip:DIP-35790N	-	-	-	figure legend:Figure 6A, Supplemental data Table S1, Figure 1|comment:"Due to the abundance of proteins in the HypAStr elution fractions, clear HypB, SlyD, and HycE bands were not observed in the Coomassie stained gel; however, tryptic digests of elution fractions followed by LC-MS/MS as well as Western blotting did confirm their presence (Fig. 1, and supplemental Table S1)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:rnJ/E4d/QF8dujq+1wLI9E2BsWA83333	intact-crc:21E5B79E60D9A7FB|rigid:mubRjQ1PkVsBzjge20zFRPmfX6I	false	strep tag:c-c	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P0A700	uniprotkb:P00551	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-6303287|uniprotkb:Q53299|uniprotkb:Q8GNQ0	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:kka1_ecolx(display_long)|uniprotkb:aphA1(gene name)|psi-mi:aphA1(display_short)|uniprotkb:nptI(gene name synonym)|uniprotkb:"APH(3')-I"(gene name synonym)|uniprotkb:Kanamycin kinase, type I(gene name synonym)|uniprotkb:Neomycin-kanamycin phosphotransferase type I(gene name synonym)	psi-mi:"MI:0096"(pull down)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:562(ecolx)|taxid:562(Escherichia coli)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303306|imex:IM-17822-16	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	go:"GO:0046677"(response to antibiotic)|go:"GO:0005524"(ATP binding)|go:"GO:0008910"(kanamycin kinase activity)|interpro:IPR002575(Aminoglycoside phosphotransferase)|interpro:IPR024165|interpro:IPR011009(Protein kinase-like)|refseq:YP_001687822.1|refseq:YP_002891173.1|refseq:YP_002995708.1|refseq:YP_008864028.1|refseq:YP_008864695.1|refseq:YP_008995202.1|refseq:YP_008997405.1|mint:P00551|refseq:WP_000018322.1|refseq:WP_063841683.1|refseq:WP_063842148.1	-	-	-	figure legend:Figure 6A, Supplemental data Table S1, Figure 1|comment:"Due to the abundance of proteins in the HypAStr elution fractions, clear HypB, SlyD, and HycE bands were not observed in the Coomassie stained gel; however, tryptic digests of elution fractions followed by LC-MS/MS as well as Western blotting did confirm their presence (Fig. 1, and supplemental Table S1)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:xSKphq0d/h1/FBcS/OADftc1fIM562	intact-crc:21E5B79E60D9A7FB|rigid:mubRjQ1PkVsBzjge20zFRPmfX6I	false	strep tag:c-c	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P0A700	uniprotkb:P07658	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-1121603|uniprotkb:P78137|uniprotkb:Q2M6M5	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:fdhf_ecoli(display_long)|uniprotkb:Formate-hydrogen-lyase-linked, selenocysteine-containing polypeptide(gene name synonym)|uniprotkb:Formate dehydrogenase-H subunit alpha(gene name synonym)|uniprotkb:fdhF(gene name)|psi-mi:fdhF(display_short)|uniprotkb:b4079(locus name)|uniprotkb:JW4040(locus name)	psi-mi:"MI:0096"(pull down)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303306|imex:IM-17822-16	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	go:"GO:0005829"(cytosol)|go:"GO:0008863"("formate dehydrogenase (NAD+) activity")|go:"GO:0015944"(formate oxidation)|go:"GO:0019628"(urate catabolic process)|go:"GO:0030151"(molybdenum ion binding)|go:"GO:0043546"(molybdopterin cofactor binding)|go:"GO:0045272"(plasma membrane respiratory chain complex I)|go:"GO:0045333"(cellular respiration)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR006478(Formate dehydrogenase, alpha subunit)|interpro:IPR006655(Molybdopterin oxidoreductase, prokaryotic, conserved site)|interpro:IPR006656(Molybdopterin oxidoreductase)|interpro:IPR006657(Molydopterin dinucleotide-binding region)|interpro:IPR006963(Molybdopterin oxidoreductase Fe4S4 region)|interpro:IPR009010(Aspartate decarboxylase-like fold)|interpro:IPR027467|interpro:IPR041924|interpro:IPR041925|rcsb pdb:1AA6|rcsb pdb:1FDI|rcsb pdb:1FDO|rcsb pdb:2IV2|refseq:WP_001300547.1|refseq:NP_418503.1|dip:DIP-9572N	-	-	-	figure legend:Figure 6A, Supplemental data Table S1, Figure 1|comment:"Due to the abundance of proteins in the HypAStr elution fractions, clear HypB, SlyD, and HycE bands were not observed in the Coomassie stained gel; however, tryptic digests of elution fractions followed by LC-MS/MS as well as Western blotting did confirm their presence (Fig. 1, and supplemental Table S1)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:zccIV3Xvzfk8vW6zGUDcNzhqssM83333	intact-crc:21E5B79E60D9A7FB|rigid:mubRjQ1PkVsBzjge20zFRPmfX6I	false	strep tag:c-c	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P0A700	uniprotkb:P0AAN3	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-558261|uniprotkb:P24190|uniprotkb:Q2MAA2|uniprotkb:Q46884|ensemblbacteria:AAC75769|ensemblbacteria:BAE76804	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:hypb_ecoli(display_long)|uniprotkb:hypB(gene name)|psi-mi:hypB(display_short)|uniprotkb:Hydrogenase isoenzymes nickel incorporation protein HypB(gene name synonym)|uniprotkb:JW2697(locus name)|uniprotkb:b2727(locus name)	psi-mi:"MI:0096"(pull down)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303306|imex:IM-17822-16	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417207.1|refseq:WP_000337665.1|dip:DIP-36428N|ensemblbacteria:AAC75769(transcript)|ensemblbacteria:b2727(gene)|ensemblbacteria:BAE76804(gene)|ensemblbacteria:BAE76804(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0051604"(protein maturation)|interpro:IPR003495("Cobalamin (vitamin B12) biosynthesis CobW-like")|interpro:IPR004392(Hydrogenase accessory protein HypB)|interpro:IPR027417	-	-	-	figure legend:Figure 6A, Supplemental data Table S1, Figure 1|comment:"Due to the abundance of proteins in the HypAStr elution fractions, clear HypB, SlyD, and HycE bands were not observed in the Coomassie stained gel; however, tryptic digests of elution fractions followed by LC-MS/MS as well as Western blotting did confirm their presence (Fig. 1, and supplemental Table S1)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:bg58XWiAv28fGfbHi898yF5HN8o83333	intact-crc:21E5B79E60D9A7FB|rigid:mubRjQ1PkVsBzjge20zFRPmfX6I	false	strep tag:c-c	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P0A700	uniprotkb:P0C8J8	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-550954|uniprotkb:P76414|intact:EBI-9148602|uniprotkb:P37191|ensemblbacteria:AAC75156|ensemblbacteria:BAA15965	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:gatz_ecoli(display_long)|uniprotkb:gatZ(gene name)|psi-mi:gatZ(display_short)|uniprotkb:b2095(locus name)|uniprotkb:JW2082(locus name)	psi-mi:"MI:0096"(pull down)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303306|imex:IM-17822-16	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_416598.1|refseq:WP_000853883.1|dip:DIP-48243N|ensemblbacteria:AAC75156(transcript)|ensemblbacteria:b2095(gene)|ensemblbacteria:BAA15965(gene)|ensemblbacteria:BAA15965(transcript)|go:"GO:0003824"(catalytic activity)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009401"(phosphoenolpyruvate-dependent sugar phosphotransferase system)|go:"GO:0019404"(galactitol catabolic process)|go:"GO:2001059"(D-tagatose 6-phosphate catabolic process)|interpro:IPR012062(D-tagatose-bisphosphate aldolase, class II, non-catalytic subunit AgaZ)|interpro:IPR013785(Aldolase-type TIM barrel)|interpro:IPR023436	-	-	caution:This sequence has been withdrawn from Uniprot.|crc64:258D25D832E42580	figure legend:Figure 6A, Supplemental data Table S1, Figure 1|comment:"Due to the abundance of proteins in the HypAStr elution fractions, clear HypB, SlyD, and HycE bands were not observed in the Coomassie stained gel; however, tryptic digests of elution fractions followed by LC-MS/MS as well as Western blotting did confirm their presence (Fig. 1, and supplemental Table S1)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:8ZWBXZiqVKLX9dXLlk7eYwqKgNk83333	intact-crc:21E5B79E60D9A7FB|rigid:mubRjQ1PkVsBzjge20zFRPmfX6I	false	strep tag:c-c	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P0A700	uniprotkb:P0A9K9	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-369251|uniprotkb:P30856|uniprotkb:Q2M714|ensemblbacteria:AAC76374|ensemblbacteria:BAE77942	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:slyd_ecoli(display_long)|uniprotkb:slyD(gene name)|psi-mi:slyD(display_short)|uniprotkb:b3349(locus name)|uniprotkb:Metallochaperone SlyD(gene name synonym)|uniprotkb:JW3311(locus name)|uniprotkb:Histidine-rich protein(gene name synonym)|uniprotkb:WHP(gene name synonym)|uniprotkb:Rotamase(gene name synonym)|uniprotkb:Sensitivity to lysis protein D(gene name synonym)	psi-mi:"MI:0096"(pull down)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303306|imex:IM-17822-16	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417808.1|refseq:WP_000861334.1|ensemblbacteria:AAC76374(transcript)|ensemblbacteria:b3349(gene)|ensemblbacteria:BAE77942(gene)|ensemblbacteria:BAE77942(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009408"(response to heat)|go:"GO:0016151"(nickel cation binding)|go:"GO:0022417"(protein maturation by protein folding)|go:"GO:0042026"(protein refolding)|go:"GO:0043963"(modulation by symbiont of host adenylate cyclase-mediated signal transduction)|go:"GO:0050821"(protein stabilization)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR001179(Peptidyl-prolyl cis-trans isomerase, FKBP-type)|rcsb pdb:2K8I|rcsb pdb:2KFW|rcsb pdb:5I7P|dip:DIP-31853N	-	-	-	figure legend:Figure 6A, Supplemental data Table S1, Figure 1|comment:"Due to the abundance of proteins in the HypAStr elution fractions, clear HypB, SlyD, and HycE bands were not observed in the Coomassie stained gel; however, tryptic digests of elution fractions followed by LC-MS/MS as well as Western blotting did confirm their presence (Fig. 1, and supplemental Table S1)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:Y/b//pPxL6aoUOf5NH2HKiB+wN883333	intact-crc:21E5B79E60D9A7FB|rigid:mubRjQ1PkVsBzjge20zFRPmfX6I	false	strep tag:c-c	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P0A700	uniprotkb:P16431	intact:EBI-6290024|ensemblbacteria:AAC75768|ensemblbacteria:BAE76803|uniprotkb:P24189|uniprotkb:Q2MAA3	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	psi-mi:hypa_ecoli(display_long)|uniprotkb:hypA(gene name)|psi-mi:hypA(display_short)|uniprotkb:b2726(locus name)|uniprotkb:JW2696(locus name)	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:"MI:0096"(pull down)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303306|imex:IM-17822-16	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC75768(transcript)|ensemblbacteria:b2726(gene)|ensemblbacteria:BAE76803(gene)|ensemblbacteria:BAE76803(transcript)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR000688(Hydrogenase expression/synthesis, HypA)|interpro:IPR020538|refseq:WP_001299100.1|dip:DIP-48022N|refseq:NP_417206.1	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	-	-	-	figure legend:Figure 6A, Supplemental data Table S1, Figure 1|comment:"Due to the abundance of proteins in the HypAStr elution fractions, clear HypB, SlyD, and HycE bands were not observed in the Coomassie stained gel; however, tryptic digests of elution fractions followed by LC-MS/MS as well as Western blotting did confirm their presence (Fig. 1, and supplemental Table S1)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:Ean6OfcE8MFqbj2442Oswl0KWBA83333	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	intact-crc:21E5B79E60D9A7FB|rigid:mubRjQ1PkVsBzjge20zFRPmfX6I	false	strep tag:c-c	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P16431	uniprotkb:P0C8J8	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-550954|uniprotkb:P76414|intact:EBI-9148602|uniprotkb:P37191|ensemblbacteria:AAC75156|ensemblbacteria:BAA15965	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:gatz_ecoli(display_long)|uniprotkb:gatZ(gene name)|psi-mi:gatZ(display_short)|uniprotkb:b2095(locus name)|uniprotkb:JW2082(locus name)	psi-mi:"MI:0096"(pull down)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303327|imex:IM-17822-17	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	refseq:NP_416598.1|refseq:WP_000853883.1|dip:DIP-48243N|ensemblbacteria:AAC75156(transcript)|ensemblbacteria:b2095(gene)|ensemblbacteria:BAA15965(gene)|ensemblbacteria:BAA15965(transcript)|go:"GO:0003824"(catalytic activity)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009401"(phosphoenolpyruvate-dependent sugar phosphotransferase system)|go:"GO:0019404"(galactitol catabolic process)|go:"GO:2001059"(D-tagatose 6-phosphate catabolic process)|interpro:IPR012062(D-tagatose-bisphosphate aldolase, class II, non-catalytic subunit AgaZ)|interpro:IPR013785(Aldolase-type TIM barrel)|interpro:IPR023436	-	-	caution:This sequence has been withdrawn from Uniprot.|crc64:258D25D832E42580	figure legend:Figure 6C, Supplemental data table S1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:8ZWBXZiqVKLX9dXLlk7eYwqKgNk83333	intact-crc:5C4B35EF862CFDF4|rigid:jAJzgTsBaO09gd8AdBgctWvf6Z8	false	strep tag:n-n	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:P16431	uniprotkb:P0A6Y9	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	intact:EBI-6303329|ensemblbacteria:AAN78519|uniprotkb:P04475	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:dnak_ecol6(display_long)|uniprotkb:dnaK(gene name)|psi-mi:dnaK(display_short)|uniprotkb:HSP70(gene name synonym)|uniprotkb:Heat shock 70 kDa protein(gene name synonym)|uniprotkb:Heat shock protein 70(gene name synonym)|uniprotkb:c0019(locus name)	psi-mi:"MI:0096"(pull down)	Chan et al. (2011)	pubmed:22016389|imex:IM-17822	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:199310(ecol6)|taxid:199310("Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-6303327|imex:IM-17822-17	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	ensemblbacteria:AAN78519(transcript)|ensemblbacteria:c0019(gene)|go:"GO:0005524"(ATP binding)|go:"GO:0006457"(protein folding)|go:"GO:0051082"(unfolded protein binding)|refseq:WP_000516135.1|interpro:IPR012725(Chaperone DnaK)|interpro:IPR018181|interpro:IPR013126(Heat shock protein 70)|interpro:IPR029047|interpro:IPR029048|interpro:IPR043129	-	-	-	figure legend:Figure 6C, Supplemental data table S1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2012/09/14	2014/10/16	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	rogid:jm8ErX3H3EOAYYuSDcddoE/NQx4199310	intact-crc:5C4B35EF862CFDF4|rigid:jAJzgTsBaO09gd8AdBgctWvf6Z8	false	strep tag:n-n	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)