#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P16497 uniprotkb:P16497 intact:EBI-6405707|ensemblbacteria:CAB13272|uniprotkb:P22863 intact:EBI-6405707|ensemblbacteria:CAB13272|uniprotkb:P22863 psi-mi:kina_bacsu(display_long)|uniprotkb:kinA(gene name)|psi-mi:kinA(display_short)|uniprotkb:Stage II sporulation protein F(gene name synonym)|uniprotkb:Stage II sporulation protein J(gene name synonym)|uniprotkb:gsiC(gene name synonym)|uniprotkb:scoD(gene name synonym)|uniprotkb:spoIIF(gene name synonym)|uniprotkb:spoIIJ(gene name synonym)|uniprotkb:BSU13990(locus name) psi-mi:kina_bacsu(display_long)|uniprotkb:kinA(gene name)|psi-mi:kinA(display_short)|uniprotkb:Stage II sporulation protein F(gene name synonym)|uniprotkb:Stage II sporulation protein J(gene name synonym)|uniprotkb:gsiC(gene name synonym)|uniprotkb:scoD(gene name synonym)|uniprotkb:spoIIF(gene name synonym)|uniprotkb:spoIIJ(gene name synonym)|uniprotkb:BSU13990(locus name) psi-mi:"MI:0424"(protein kinase assay) Cunningham et al. (2009) pubmed:19040634|imex:IM-18252 taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0903"(mpidb) intact:EBI-6405704|mpidb:MPIDB-INT-292|imex:IM-18252-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0502"(enzyme target) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblbacteria:BSU_13990(gene)|ensemblbacteria:CAB13272(transcript)|go:"GO:0000155"(phosphorelay sensor kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0042802"(identical protein binding)|interpro:IPR000014(PAS)|interpro:IPR001610(PAC motif)|interpro:IPR003661(Signal transduction histidine kinase, subgroup 1, dimerisation and phosphoacceptor region)|interpro:IPR004358(Signal transduction histidine kinase-related protein, C-terminal)|interpro:IPR005467(Signal transduction histidine kinase, core)|interpro:IPR013655(PAS fold-3)|interpro:IPR013767(PAS fold)|interpro:IPR035965|interpro:IPR036097|interpro:IPR036890|rcsb pdb:2VLG|interpro:IPR003594(ATP-binding region, ATPase-like)|refseq:NP_389282.1|refseq:WP_003245779.1|dip:DIP-58966N ensemblbacteria:BSU_13990(gene)|ensemblbacteria:CAB13272(transcript)|go:"GO:0000155"(phosphorelay sensor kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0042802"(identical protein binding)|interpro:IPR000014(PAS)|interpro:IPR001610(PAC motif)|interpro:IPR003661(Signal transduction histidine kinase, subgroup 1, dimerisation and phosphoacceptor region)|interpro:IPR004358(Signal transduction histidine kinase-related protein, C-terminal)|interpro:IPR005467(Signal transduction histidine kinase, core)|interpro:IPR013655(PAS fold-3)|interpro:IPR013767(PAS fold)|interpro:IPR035965|interpro:IPR036097|interpro:IPR036890|rcsb pdb:2VLG|interpro:IPR003594(ATP-binding region, ATPase-like)|refseq:NP_389282.1|refseq:WP_003245779.1|dip:DIP-58966N - crc64:A8BB97FE844B32EE crc64:A8BB97FE844B32EE figure legend:Fig. 1, 2B|comment:"Higher concentrations of Sda are required to inhibit the autophosphorylation activities of KinA(P410L) and KinA(P410L/F436S) than to inhibit wild-type KinA"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2012/11/30 2014/10/16 rogid:A0Vvk4TcQoLyGVes0Txhdafdszk224308 rogid:A0Vvk4TcQoLyGVes0Txhdafdszk224308 intact-crc:0DD0E964BB140896|rigid:NsMEaaiR6kUzBjz308iYpBa/fz8 false sufficient binding region:383-606(IPR005467)|his tag:?-? sufficient binding region:383-461(IPR009082)|his tag:?-? - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) uniprotkb:P16497 uniprotkb:Q7WY62 intact:EBI-6405707|ensemblbacteria:CAB13272|uniprotkb:P22863 intact:EBI-6405714|ensemblbacteria:CAE01463 psi-mi:kina_bacsu(display_long)|uniprotkb:kinA(gene name)|psi-mi:kinA(display_short)|uniprotkb:Stage II sporulation protein F(gene name synonym)|uniprotkb:Stage II sporulation protein J(gene name synonym)|uniprotkb:gsiC(gene name synonym)|uniprotkb:scoD(gene name synonym)|uniprotkb:spoIIF(gene name synonym)|uniprotkb:spoIIJ(gene name synonym)|uniprotkb:BSU13990(locus name) psi-mi:sda_bacsu(display_long)|uniprotkb:sda(gene name)|psi-mi:sda(display_short)|uniprotkb:Histidine kinase KinA inhibitor(gene name synonym)|uniprotkb:BSU25690(locus name) psi-mi:"MI:0424"(protein kinase assay) Cunningham et al. (2009) pubmed:19040634|imex:IM-18252 taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0903"(mpidb) intact:EBI-6405704|mpidb:MPIDB-INT-292|imex:IM-18252-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0586"(inhibitor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblbacteria:BSU_13990(gene)|ensemblbacteria:CAB13272(transcript)|go:"GO:0000155"(phosphorelay sensor kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0042802"(identical protein binding)|interpro:IPR000014(PAS)|interpro:IPR001610(PAC motif)|interpro:IPR003661(Signal transduction histidine kinase, subgroup 1, dimerisation and phosphoacceptor region)|interpro:IPR004358(Signal transduction histidine kinase-related protein, C-terminal)|interpro:IPR005467(Signal transduction histidine kinase, core)|interpro:IPR013655(PAS fold-3)|interpro:IPR013767(PAS fold)|interpro:IPR035965|interpro:IPR036097|interpro:IPR036890|rcsb pdb:2VLG|interpro:IPR003594(ATP-binding region, ATPase-like)|refseq:NP_389282.1|refseq:WP_003245779.1|dip:DIP-58966N ensemblbacteria:BSU_25690(gene)|ensemblbacteria:CAE01463(transcript)|go:"GO:0004860"(protein kinase inhibitor activity)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|refseq:WP_010886571.1|interpro:IPR015064(Sporulation inhibitor A)|rcsb pdb:1PV0|rcsb pdb:3FYR|refseq:YP_054588.1|interpro:IPR036916 - crc64:A8BB97FE844B32EE - figure legend:Fig. 1, 2B|comment:"Higher concentrations of Sda are required to inhibit the autophosphorylation activities of KinA(P410L) and KinA(P410L/F436S) than to inhibit wild-type KinA"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2012/11/30 2014/10/16 rogid:A0Vvk4TcQoLyGVes0Txhdafdszk224308 rogid:B7r35Powpwtpo6qwLzYAamiAj10224308 intact-crc:0DD0E964BB140896|rigid:NsMEaaiR6kUzBjz308iYpBa/fz8 false sufficient binding region:383-606(IPR005467)|his tag:?-? - - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) uniprotkb:P16497 uniprotkb:P16497 intact:EBI-6405707|ensemblbacteria:CAB13272|uniprotkb:P22863 intact:EBI-6405707|ensemblbacteria:CAB13272|uniprotkb:P22863 psi-mi:kina_bacsu(display_long)|uniprotkb:kinA(gene name)|psi-mi:kinA(display_short)|uniprotkb:Stage II sporulation protein F(gene name synonym)|uniprotkb:Stage II sporulation protein J(gene name synonym)|uniprotkb:gsiC(gene name synonym)|uniprotkb:scoD(gene name synonym)|uniprotkb:spoIIF(gene name synonym)|uniprotkb:spoIIJ(gene name synonym)|uniprotkb:BSU13990(locus name) psi-mi:kina_bacsu(display_long)|uniprotkb:kinA(gene name)|psi-mi:kinA(display_short)|uniprotkb:Stage II sporulation protein F(gene name synonym)|uniprotkb:Stage II sporulation protein J(gene name synonym)|uniprotkb:gsiC(gene name synonym)|uniprotkb:scoD(gene name synonym)|uniprotkb:spoIIF(gene name synonym)|uniprotkb:spoIIJ(gene name synonym)|uniprotkb:BSU13990(locus name) psi-mi:"MI:0424"(protein kinase assay) Cunningham et al. (2009) pubmed:19040634|imex:IM-18252 taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) psi-mi:"MI:0217"(phosphorylation reaction) psi-mi:"MI:0903"(mpidb) intact:EBI-6508565|mpidb:MPIDB-INT-292|imex:IM-18252-2 - - psi-mi:"MI:0502"(enzyme target) psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblbacteria:BSU_13990(gene)|ensemblbacteria:CAB13272(transcript)|go:"GO:0000155"(phosphorelay sensor kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0042802"(identical protein binding)|interpro:IPR000014(PAS)|interpro:IPR001610(PAC motif)|interpro:IPR003661(Signal transduction histidine kinase, subgroup 1, dimerisation and phosphoacceptor region)|interpro:IPR004358(Signal transduction histidine kinase-related protein, C-terminal)|interpro:IPR005467(Signal transduction histidine kinase, core)|interpro:IPR013655(PAS fold-3)|interpro:IPR013767(PAS fold)|interpro:IPR035965|interpro:IPR036097|interpro:IPR036890|rcsb pdb:2VLG|interpro:IPR003594(ATP-binding region, ATPase-like)|refseq:NP_389282.1|refseq:WP_003245779.1|dip:DIP-58966N ensemblbacteria:BSU_13990(gene)|ensemblbacteria:CAB13272(transcript)|go:"GO:0000155"(phosphorelay sensor kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0042802"(identical protein binding)|interpro:IPR000014(PAS)|interpro:IPR001610(PAC motif)|interpro:IPR003661(Signal transduction histidine kinase, subgroup 1, dimerisation and phosphoacceptor region)|interpro:IPR004358(Signal transduction histidine kinase-related protein, C-terminal)|interpro:IPR005467(Signal transduction histidine kinase, core)|interpro:IPR013655(PAS fold-3)|interpro:IPR013767(PAS fold)|interpro:IPR035965|interpro:IPR036097|interpro:IPR036890|rcsb pdb:2VLG|interpro:IPR003594(ATP-binding region, ATPase-like)|refseq:NP_389282.1|refseq:WP_003245779.1|dip:DIP-58966N - crc64:A8BB97FE844B32EE crc64:A8BB97FE844B32EE figure legend:Fig. 1|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2012/11/30 2014/10/16 rogid:A0Vvk4TcQoLyGVes0Txhdafdszk224308 rogid:A0Vvk4TcQoLyGVes0Txhdafdszk224308 intact-crc:3E9FAD325C6C67F8|rigid:5GBWXXT7IZviJRoZgsQRz7PCPJY false his tag:?-?|sufficient binding region:383-461(IPR009082) his tag:?-?|sufficient binding region:383-606(IPR005467) - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) uniprotkb:P06628 uniprotkb:P16497 intact:EBI-6418009|ensemblbacteria:CAB15730 intact:EBI-6405707|ensemblbacteria:CAB13272|uniprotkb:P22863 psi-mi:sp0f_bacsu(display_long)|uniprotkb:spo0F(gene name)|psi-mi:spo0F(display_short)|uniprotkb:Stage 0 sporulation protein F(gene name synonym)|uniprotkb:BSU37130(locus name) psi-mi:kina_bacsu(display_long)|uniprotkb:kinA(gene name)|psi-mi:kinA(display_short)|uniprotkb:Stage II sporulation protein F(gene name synonym)|uniprotkb:Stage II sporulation protein J(gene name synonym)|uniprotkb:gsiC(gene name synonym)|uniprotkb:scoD(gene name synonym)|uniprotkb:spoIIF(gene name synonym)|uniprotkb:spoIIJ(gene name synonym)|uniprotkb:BSU13990(locus name) psi-mi:"MI:0841"(phosphotransferase assay) Cunningham et al. (2009) pubmed:19040634|imex:IM-18252 taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) psi-mi:"MI:0844"(phosphotransfer reaction) psi-mi:"MI:0903"(mpidb) intact:EBI-6508800|mpidb:MPIDB-INT-292|imex:IM-18252-8 - - psi-mi:"MI:0843"(phosphate acceptor) psi-mi:"MI:1084"(photon donor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblbacteria:BSU_37130(gene)|ensemblbacteria:CAB15730(transcript)|go:"GO:0000160"(phosphorelay signal transduction system)|go:"GO:0005737"(cytoplasm)|go:"GO:0016301"(kinase activity)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0046872"(metal ion binding)|interpro:IPR001789(Signal transduction response regulator, receiver region)|interpro:IPR011006(CheY-like)|rcsb pdb:1F51|rcsb pdb:1FSP|rcsb pdb:1NAT|rcsb pdb:1PEY|rcsb pdb:1PUX|rcsb pdb:1SRR|rcsb pdb:2FSP|rcsb pdb:2FTK|rcsb pdb:2JVI|rcsb pdb:2JVJ|rcsb pdb:2JVK|rcsb pdb:3Q15|refseq:NP_391594.1|refseq:WP_003227621.1|dip:DIP-58965N ensemblbacteria:BSU_13990(gene)|ensemblbacteria:CAB13272(transcript)|go:"GO:0000155"(phosphorelay sensor kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0042802"(identical protein binding)|interpro:IPR000014(PAS)|interpro:IPR001610(PAC motif)|interpro:IPR003661(Signal transduction histidine kinase, subgroup 1, dimerisation and phosphoacceptor region)|interpro:IPR004358(Signal transduction histidine kinase-related protein, C-terminal)|interpro:IPR005467(Signal transduction histidine kinase, core)|interpro:IPR013655(PAS fold-3)|interpro:IPR013767(PAS fold)|interpro:IPR035965|interpro:IPR036097|interpro:IPR036890|rcsb pdb:2VLG|interpro:IPR003594(ATP-binding region, ATPase-like)|refseq:NP_389282.1|refseq:WP_003245779.1|dip:DIP-58966N - crc64:4130377E3558D698 crc64:A8BB97FE844B32EE figure legend:Fig. 10|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2012/11/30 2014/10/16 rogid:NvHnCHOWob2R2rzN4xJpDZpSTj4224308 rogid:A0Vvk4TcQoLyGVes0Txhdafdszk224308 intact-crc:ACA67B0010B537B0|rigid:DflNpK2wOP+XsWKbbvTtlpC4/5o false his tag:c-c 32p radiolabel:?-?|his tag:n-n - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) uniprotkb:P16497 uniprotkb:Q7WY62 intact:EBI-6405707|ensemblbacteria:CAB13272|uniprotkb:P22863 intact:EBI-6405714|ensemblbacteria:CAE01463 psi-mi:kina_bacsu(display_long)|uniprotkb:kinA(gene name)|psi-mi:kinA(display_short)|uniprotkb:Stage II sporulation protein F(gene name synonym)|uniprotkb:Stage II sporulation protein J(gene name synonym)|uniprotkb:gsiC(gene name synonym)|uniprotkb:scoD(gene name synonym)|uniprotkb:spoIIF(gene name synonym)|uniprotkb:spoIIJ(gene name synonym)|uniprotkb:BSU13990(locus name) psi-mi:sda_bacsu(display_long)|uniprotkb:sda(gene name)|psi-mi:sda(display_short)|uniprotkb:Histidine kinase KinA inhibitor(gene name synonym)|uniprotkb:BSU25690(locus name) psi-mi:"MI:0841"(phosphotransferase assay) Cunningham et al. (2009) pubmed:19040634|imex:IM-18252 taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0903"(mpidb) intact:EBI-6508811|mpidb:MPIDB-INT-292|imex:IM-18252-9 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:1084"(photon donor) psi-mi:"MI:0586"(inhibitor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblbacteria:BSU_13990(gene)|ensemblbacteria:CAB13272(transcript)|go:"GO:0000155"(phosphorelay sensor kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0042802"(identical protein binding)|interpro:IPR000014(PAS)|interpro:IPR001610(PAC motif)|interpro:IPR003661(Signal transduction histidine kinase, subgroup 1, dimerisation and phosphoacceptor region)|interpro:IPR004358(Signal transduction histidine kinase-related protein, C-terminal)|interpro:IPR005467(Signal transduction histidine kinase, core)|interpro:IPR013655(PAS fold-3)|interpro:IPR013767(PAS fold)|interpro:IPR035965|interpro:IPR036097|interpro:IPR036890|rcsb pdb:2VLG|interpro:IPR003594(ATP-binding region, ATPase-like)|refseq:NP_389282.1|refseq:WP_003245779.1|dip:DIP-58966N ensemblbacteria:BSU_25690(gene)|ensemblbacteria:CAE01463(transcript)|go:"GO:0004860"(protein kinase inhibitor activity)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|refseq:WP_010886571.1|interpro:IPR015064(Sporulation inhibitor A)|rcsb pdb:1PV0|rcsb pdb:3FYR|refseq:YP_054588.1|interpro:IPR036916 - crc64:A8BB97FE844B32EE - figure legend:Fig. 10|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2012/11/30 2014/10/16 rogid:A0Vvk4TcQoLyGVes0Txhdafdszk224308 rogid:B7r35Powpwtpo6qwLzYAamiAj10224308 intact-crc:1B7C24F7CB38631D|rigid:nbKIb+2GLkfk1hteUtWqTf8pPUg false his tag:n-n|32p radiolabel:?-? - - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) uniprotkb:P16497 uniprotkb:P06628 intact:EBI-6405707|ensemblbacteria:CAB13272|uniprotkb:P22863 intact:EBI-6418009|ensemblbacteria:CAB15730 psi-mi:kina_bacsu(display_long)|uniprotkb:kinA(gene name)|psi-mi:kinA(display_short)|uniprotkb:Stage II sporulation protein F(gene name synonym)|uniprotkb:Stage II sporulation protein J(gene name synonym)|uniprotkb:gsiC(gene name synonym)|uniprotkb:scoD(gene name synonym)|uniprotkb:spoIIF(gene name synonym)|uniprotkb:spoIIJ(gene name synonym)|uniprotkb:BSU13990(locus name) psi-mi:sp0f_bacsu(display_long)|uniprotkb:spo0F(gene name)|psi-mi:spo0F(display_short)|uniprotkb:Stage 0 sporulation protein F(gene name synonym)|uniprotkb:BSU37130(locus name) psi-mi:"MI:0841"(phosphotransferase assay) Cunningham et al. (2009) pubmed:19040634|imex:IM-18252 taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0903"(mpidb) intact:EBI-6508811|mpidb:MPIDB-INT-292|imex:IM-18252-9 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:1084"(photon donor) psi-mi:"MI:0843"(phosphate acceptor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblbacteria:BSU_13990(gene)|ensemblbacteria:CAB13272(transcript)|go:"GO:0000155"(phosphorelay sensor kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0042802"(identical protein binding)|interpro:IPR000014(PAS)|interpro:IPR001610(PAC motif)|interpro:IPR003661(Signal transduction histidine kinase, subgroup 1, dimerisation and phosphoacceptor region)|interpro:IPR004358(Signal transduction histidine kinase-related protein, C-terminal)|interpro:IPR005467(Signal transduction histidine kinase, core)|interpro:IPR013655(PAS fold-3)|interpro:IPR013767(PAS fold)|interpro:IPR035965|interpro:IPR036097|interpro:IPR036890|rcsb pdb:2VLG|interpro:IPR003594(ATP-binding region, ATPase-like)|refseq:NP_389282.1|refseq:WP_003245779.1|dip:DIP-58966N ensemblbacteria:BSU_37130(gene)|ensemblbacteria:CAB15730(transcript)|go:"GO:0000160"(phosphorelay signal transduction system)|go:"GO:0005737"(cytoplasm)|go:"GO:0016301"(kinase activity)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0046872"(metal ion binding)|interpro:IPR001789(Signal transduction response regulator, receiver region)|interpro:IPR011006(CheY-like)|rcsb pdb:1F51|rcsb pdb:1FSP|rcsb pdb:1NAT|rcsb pdb:1PEY|rcsb pdb:1PUX|rcsb pdb:1SRR|rcsb pdb:2FSP|rcsb pdb:2FTK|rcsb pdb:2JVI|rcsb pdb:2JVJ|rcsb pdb:2JVK|rcsb pdb:3Q15|refseq:NP_391594.1|refseq:WP_003227621.1|dip:DIP-58965N - crc64:A8BB97FE844B32EE crc64:4130377E3558D698 figure legend:Fig. 10|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2012/11/30 2014/10/16 rogid:A0Vvk4TcQoLyGVes0Txhdafdszk224308 rogid:NvHnCHOWob2R2rzN4xJpDZpSTj4224308 intact-crc:1B7C24F7CB38631D|rigid:nbKIb+2GLkfk1hteUtWqTf8pPUg false his tag:n-n|32p radiolabel:?-? his tag:c-c - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) uniprotkb:P16497 - intact:EBI-6405707|ensemblbacteria:CAB13272|uniprotkb:P22863 - psi-mi:kina_bacsu(display_long)|uniprotkb:kinA(gene name)|psi-mi:kinA(display_short)|uniprotkb:Stage II sporulation protein F(gene name synonym)|uniprotkb:Stage II sporulation protein J(gene name synonym)|uniprotkb:gsiC(gene name synonym)|uniprotkb:scoD(gene name synonym)|uniprotkb:spoIIF(gene name synonym)|uniprotkb:spoIIJ(gene name synonym)|uniprotkb:BSU13990(locus name) - psi-mi:"MI:0424"(protein kinase assay) Cunningham et al. (2009) pubmed:19040634|imex:IM-18252 taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) - psi-mi:"MI:0217"(phosphorylation reaction) psi-mi:"MI:0903"(mpidb) intact:EBI-6508771|mpidb:MPIDB-INT-292|imex:IM-18252-6 - - psi-mi:"MI:0898"(putative self) - psi-mi:"MI:0898"(putative self) - psi-mi:"MI:0326"(protein) - ensemblbacteria:BSU_13990(gene)|ensemblbacteria:CAB13272(transcript)|go:"GO:0000155"(phosphorelay sensor kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0042802"(identical protein binding)|interpro:IPR000014(PAS)|interpro:IPR001610(PAC motif)|interpro:IPR003661(Signal transduction histidine kinase, subgroup 1, dimerisation and phosphoacceptor region)|interpro:IPR004358(Signal transduction histidine kinase-related protein, C-terminal)|interpro:IPR005467(Signal transduction histidine kinase, core)|interpro:IPR013655(PAS fold-3)|interpro:IPR013767(PAS fold)|interpro:IPR035965|interpro:IPR036097|interpro:IPR036890|rcsb pdb:2VLG|interpro:IPR003594(ATP-binding region, ATPase-like)|refseq:NP_389282.1|refseq:WP_003245779.1|dip:DIP-58966N - - crc64:A8BB97FE844B32EE - figure legend:Fig. 8|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2012/11/30 2014/10/16 rogid:A0Vvk4TcQoLyGVes0Txhdafdszk224308 - intact-crc:86FCC0852A87F791 false his tag:n-n|tag:606-606 - - - psi-mi:"MI:0866"(tag visualisation) - uniprotkb:P16497 uniprotkb:Q7WY62 intact:EBI-6405707|ensemblbacteria:CAB13272|uniprotkb:P22863 intact:EBI-6405714|ensemblbacteria:CAE01463 psi-mi:kina_bacsu(display_long)|uniprotkb:kinA(gene name)|psi-mi:kinA(display_short)|uniprotkb:Stage II sporulation protein F(gene name synonym)|uniprotkb:Stage II sporulation protein J(gene name synonym)|uniprotkb:gsiC(gene name synonym)|uniprotkb:scoD(gene name synonym)|uniprotkb:spoIIF(gene name synonym)|uniprotkb:spoIIJ(gene name synonym)|uniprotkb:BSU13990(locus name) psi-mi:sda_bacsu(display_long)|uniprotkb:sda(gene name)|psi-mi:sda(display_short)|uniprotkb:Histidine kinase KinA inhibitor(gene name synonym)|uniprotkb:BSU25690(locus name) psi-mi:"MI:0424"(protein kinase assay) Cunningham et al. (2009) pubmed:19040634|imex:IM-18252 taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0903"(mpidb) intact:EBI-6508783|mpidb:MPIDB-INT-292|imex:IM-18252-7 - - psi-mi:"MI:0898"(putative self) psi-mi:"MI:0586"(inhibitor) psi-mi:"MI:0898"(putative self) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblbacteria:BSU_13990(gene)|ensemblbacteria:CAB13272(transcript)|go:"GO:0000155"(phosphorelay sensor kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0042802"(identical protein binding)|interpro:IPR000014(PAS)|interpro:IPR001610(PAC motif)|interpro:IPR003661(Signal transduction histidine kinase, subgroup 1, dimerisation and phosphoacceptor region)|interpro:IPR004358(Signal transduction histidine kinase-related protein, C-terminal)|interpro:IPR005467(Signal transduction histidine kinase, core)|interpro:IPR013655(PAS fold-3)|interpro:IPR013767(PAS fold)|interpro:IPR035965|interpro:IPR036097|interpro:IPR036890|rcsb pdb:2VLG|interpro:IPR003594(ATP-binding region, ATPase-like)|refseq:NP_389282.1|refseq:WP_003245779.1|dip:DIP-58966N ensemblbacteria:BSU_25690(gene)|ensemblbacteria:CAE01463(transcript)|go:"GO:0004860"(protein kinase inhibitor activity)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|refseq:WP_010886571.1|interpro:IPR015064(Sporulation inhibitor A)|rcsb pdb:1PV0|rcsb pdb:3FYR|refseq:YP_054588.1|interpro:IPR036916 - crc64:A8BB97FE844B32EE - figure legend:Fig. 8|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2012/11/30 2014/10/16 rogid:A0Vvk4TcQoLyGVes0Txhdafdszk224308 rogid:B7r35Powpwtpo6qwLzYAamiAj10224308 intact-crc:927814BA271F023D|rigid:NsMEaaiR6kUzBjz308iYpBa/fz8 false his tag:n-n|tag:606-606 - - - psi-mi:"MI:0866"(tag visualisation) psi-mi:"MI:0866"(tag visualisation) uniprotkb:Q7WY62 uniprotkb:P16497 intact:EBI-6405714|ensemblbacteria:CAE01463 intact:EBI-6405707|ensemblbacteria:CAB13272|uniprotkb:P22863 psi-mi:sda_bacsu(display_long)|uniprotkb:sda(gene name)|psi-mi:sda(display_short)|uniprotkb:Histidine kinase KinA inhibitor(gene name synonym)|uniprotkb:BSU25690(locus name) psi-mi:kina_bacsu(display_long)|uniprotkb:kinA(gene name)|psi-mi:kinA(display_short)|uniprotkb:Stage II sporulation protein F(gene name synonym)|uniprotkb:Stage II sporulation protein J(gene name synonym)|uniprotkb:gsiC(gene name synonym)|uniprotkb:scoD(gene name synonym)|uniprotkb:spoIIF(gene name synonym)|uniprotkb:spoIIJ(gene name synonym)|uniprotkb:BSU13990(locus name) psi-mi:"MI:0096"(pull down) Cunningham et al. (2009) pubmed:19040634|imex:IM-18252 taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0903"(mpidb) intact:EBI-6508655|mpidb:MPIDB-INT-292|imex:IM-18252-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblbacteria:BSU_25690(gene)|ensemblbacteria:CAE01463(transcript)|go:"GO:0004860"(protein kinase inhibitor activity)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|refseq:WP_010886571.1|interpro:IPR015064(Sporulation inhibitor A)|rcsb pdb:1PV0|rcsb pdb:3FYR|refseq:YP_054588.1|interpro:IPR036916 ensemblbacteria:BSU_13990(gene)|ensemblbacteria:CAB13272(transcript)|go:"GO:0000155"(phosphorelay sensor kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0042802"(identical protein binding)|interpro:IPR000014(PAS)|interpro:IPR001610(PAC motif)|interpro:IPR003661(Signal transduction histidine kinase, subgroup 1, dimerisation and phosphoacceptor region)|interpro:IPR004358(Signal transduction histidine kinase-related protein, C-terminal)|interpro:IPR005467(Signal transduction histidine kinase, core)|interpro:IPR013655(PAS fold-3)|interpro:IPR013767(PAS fold)|interpro:IPR035965|interpro:IPR036097|interpro:IPR036890|rcsb pdb:2VLG|interpro:IPR003594(ATP-binding region, ATPase-like)|refseq:NP_389282.1|refseq:WP_003245779.1|dip:DIP-58966N - - crc64:A8BB97FE844B32EE figure legend:Fig. 2A|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2012/11/30 2014/10/16 rogid:B7r35Powpwtpo6qwLzYAamiAj10224308 rogid:A0Vvk4TcQoLyGVes0Txhdafdszk224308 intact-crc:23EEA91D28F08E9B|rigid:NsMEaaiR6kUzBjz308iYpBa/fz8 false - mutation decreasing interaction strength:410-410,436-436|mutation decreasing interaction strength:410-410|his tag:?-?|sufficient binding region:383-606(IPR005467) - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining) uniprotkb:P16497 uniprotkb:Q7WY62 intact:EBI-6405707|ensemblbacteria:CAB13272|uniprotkb:P22863 intact:EBI-6405714|ensemblbacteria:CAE01463 psi-mi:kina_bacsu(display_long)|uniprotkb:kinA(gene name)|psi-mi:kinA(display_short)|uniprotkb:Stage II sporulation protein F(gene name synonym)|uniprotkb:Stage II sporulation protein J(gene name synonym)|uniprotkb:gsiC(gene name synonym)|uniprotkb:scoD(gene name synonym)|uniprotkb:spoIIF(gene name synonym)|uniprotkb:spoIIJ(gene name synonym)|uniprotkb:BSU13990(locus name) psi-mi:sda_bacsu(display_long)|uniprotkb:sda(gene name)|psi-mi:sda(display_short)|uniprotkb:Histidine kinase KinA inhibitor(gene name synonym)|uniprotkb:BSU25690(locus name) psi-mi:"MI:0096"(pull down) Cunningham et al. (2009) pubmed:19040634|imex:IM-18252 taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0903"(mpidb) intact:EBI-6508679|mpidb:MPIDB-INT-292|imex:IM-18252-4 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblbacteria:BSU_13990(gene)|ensemblbacteria:CAB13272(transcript)|go:"GO:0000155"(phosphorelay sensor kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0042802"(identical protein binding)|interpro:IPR000014(PAS)|interpro:IPR001610(PAC motif)|interpro:IPR003661(Signal transduction histidine kinase, subgroup 1, dimerisation and phosphoacceptor region)|interpro:IPR004358(Signal transduction histidine kinase-related protein, C-terminal)|interpro:IPR005467(Signal transduction histidine kinase, core)|interpro:IPR013655(PAS fold-3)|interpro:IPR013767(PAS fold)|interpro:IPR035965|interpro:IPR036097|interpro:IPR036890|rcsb pdb:2VLG|interpro:IPR003594(ATP-binding region, ATPase-like)|refseq:NP_389282.1|refseq:WP_003245779.1|dip:DIP-58966N ensemblbacteria:BSU_25690(gene)|ensemblbacteria:CAE01463(transcript)|go:"GO:0004860"(protein kinase inhibitor activity)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|refseq:WP_010886571.1|interpro:IPR015064(Sporulation inhibitor A)|rcsb pdb:1PV0|rcsb pdb:3FYR|refseq:YP_054588.1|interpro:IPR036916 - crc64:A8BB97FE844B32EE - figure legend:Fig. 4, 9|comment:"Sda Ser12Cys substitution restored the interaction of Sda with KinA(Tyr431Cys)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2012/11/30 2014/10/16 rogid:A0Vvk4TcQoLyGVes0Txhdafdszk224308 rogid:B7r35Powpwtpo6qwLzYAamiAj10224308 intact-crc:E689EB0970401DFA|rigid:NsMEaaiR6kUzBjz308iYpBa/fz8 false his tag:n-n|tag:606-606|mutation decreasing interaction strength:431-431 - - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) uniprotkb:Q7WY62 uniprotkb:P16497 intact:EBI-6405714|ensemblbacteria:CAE01463 intact:EBI-6405707|ensemblbacteria:CAB13272|uniprotkb:P22863 psi-mi:sda_bacsu(display_long)|uniprotkb:sda(gene name)|psi-mi:sda(display_short)|uniprotkb:Histidine kinase KinA inhibitor(gene name synonym)|uniprotkb:BSU25690(locus name) psi-mi:kina_bacsu(display_long)|uniprotkb:kinA(gene name)|psi-mi:kinA(display_short)|uniprotkb:Stage II sporulation protein F(gene name synonym)|uniprotkb:Stage II sporulation protein J(gene name synonym)|uniprotkb:gsiC(gene name synonym)|uniprotkb:scoD(gene name synonym)|uniprotkb:spoIIF(gene name synonym)|uniprotkb:spoIIJ(gene name synonym)|uniprotkb:BSU13990(locus name) psi-mi:"MI:0030"(cross-linking study) Cunningham et al. (2009) pubmed:19040634|imex:IM-18252 taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) taxid:224308(bacsu)|taxid:224308(Bacillus subtilis) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0903"(mpidb) intact:EBI-6508707|mpidb:MPIDB-INT-292|imex:IM-18252-5 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblbacteria:BSU_25690(gene)|ensemblbacteria:CAE01463(transcript)|go:"GO:0004860"(protein kinase inhibitor activity)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|refseq:WP_010886571.1|interpro:IPR015064(Sporulation inhibitor A)|rcsb pdb:1PV0|rcsb pdb:3FYR|refseq:YP_054588.1|interpro:IPR036916 ensemblbacteria:BSU_13990(gene)|ensemblbacteria:CAB13272(transcript)|go:"GO:0000155"(phosphorelay sensor kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0042802"(identical protein binding)|interpro:IPR000014(PAS)|interpro:IPR001610(PAC motif)|interpro:IPR003661(Signal transduction histidine kinase, subgroup 1, dimerisation and phosphoacceptor region)|interpro:IPR004358(Signal transduction histidine kinase-related protein, C-terminal)|interpro:IPR005467(Signal transduction histidine kinase, core)|interpro:IPR013655(PAS fold-3)|interpro:IPR013767(PAS fold)|interpro:IPR035965|interpro:IPR036097|interpro:IPR036890|rcsb pdb:2VLG|interpro:IPR003594(ATP-binding region, ATPase-like)|refseq:NP_389282.1|refseq:WP_003245779.1|dip:DIP-58966N - - crc64:A8BB97FE844B32EE figure legend:Fig. 4|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2012/11/30 2014/10/16 rogid:B7r35Powpwtpo6qwLzYAamiAj10224308 rogid:A0Vvk4TcQoLyGVes0Txhdafdszk224308 intact-crc:162DA8C2B5ADE33B|rigid:NsMEaaiR6kUzBjz308iYpBa/fz8 false - mutation:431-431|his tag:n-n|sufficient binding region:383-606|mutation:413-413 - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot)