#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q60598	uniprotkb:P61157	intact:EBI-397955|intact:EBI-4281621|uniprotkb:Q3UGC2|ensembl:ENSMUSP00000099368	intact:EBI-351419|uniprotkb:A5PJ90|uniprotkb:P32391|uniprotkb:Q08DS8|ensembl:ENSBTAP00000066597	psi-mi:src8_mouse(display_long)|uniprotkb:Cttn(gene name)|psi-mi:Cttn(display_short)|uniprotkb:Ems1(gene name synonym)	psi-mi:arp3_bovin(display_long)|uniprotkb:ACTR3(gene name)|psi-mi:ACTR3(display_short)|uniprotkb:ARP3(gene name synonym)|uniprotkb:Actin-like protein 3(gene name synonym)|uniprotkb:Actin-2(gene name synonym)	psi-mi:"MI:0096"(pull down)	Uruno et al. (2003)	pubmed:12732638|imex:IM-18823	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1222"(mbinfo)	intact:EBI-6264483|imex:IM-18823-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_031829.2|ensembl:ENSMUSG00000031078(gene)|ensembl:ENSMUST00000103079(transcript)|go:"GO:0001726"(ruffle)|go:"GO:0002102"(podosome)|go:"GO:0005522"(profilin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006898"(receptor-mediated endocytosis)|go:"GO:0006930"(substrate-dependent cell migration, cell extension)|go:"GO:0008076"(voltage-gated potassium channel complex)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030054"(cell junction)|go:"GO:0030427"(site of polarized growth)|go:"GO:0030516"(regulation of axon extension)|go:"GO:0030833"(regulation of actin filament polymerization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:1990023"(mitotic spindle midzone)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001452(Src homology-3)|interpro:IPR003134(Hs1/Cortactin)|interpro:IPR015503(Cortactin)|interpro:IPR035716|go:"GO:0030863"(cortical cytoskeleton)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031532"(actin cytoskeleton reorganization)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0045987"(positive regulation of smooth muscle contraction)|go:"GO:0048041"(focal adhesion assembly)|go:"GO:0048812"(neuron projection morphogenesis)|go:"GO:0048870"(cell motility)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051015"(actin filament binding)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0070064"(proline-rich region binding)|go:"GO:0071933"(Arp2/3 complex binding)|go:"GO:0097062"(dendritic spine maintenance)|go:"GO:0097581"(lamellipodium organization)|go:"GO:0098871"(postsynaptic actin cytoskeleton)|go:"GO:0098885"(modification of postsynaptic actin cytoskeleton)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903146"(regulation of autophagy of mitochondrion)|interpro:IPR036028|mint:Q60598|rcsb pdb:2F9X|rcsb pdb:3ULR|rcsb pdb:5NV1|rcsb pdb:5NVJ|rcsb pdb:5NXJ|reactome:R-MMU-8856828|dip:DIP-31562N	refseq:NP_776651.1|ensembl:ENSBTAG00000003401(gene)|ensembl:ENSBTAT00000070638(transcript)|go:"GO:0003779"(actin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005885"(Arp2/3 protein complex)|go:"GO:0010592"(positive regulation of lamellipodium assembly)|go:"GO:0034314"(Arp2/3 complex-mediated actin nucleation)|go:"GO:0035861"(site of double-strand break)|go:"GO:0042995"(cell projection)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0060271"(cilium assembly)|interpro:IPR004000(Actin/actin-like)|interpro:IPR015623(Actin-related protein 3)|interpro:IPR020902|interpro:IPR043129|rcsb pdb:1K8K|rcsb pdb:1TYQ|rcsb pdb:1U2V|rcsb pdb:2P9I|rcsb pdb:2P9K|rcsb pdb:2P9L|rcsb pdb:2P9N|rcsb pdb:2P9P|rcsb pdb:2P9S|rcsb pdb:2P9U|rcsb pdb:3DXK|rcsb pdb:3DXM|rcsb pdb:3RSE|rcsb pdb:3UKR|rcsb pdb:3UKU|rcsb pdb:3ULE|rcsb pdb:4JD2|rcsb pdb:4XEI|rcsb pdb:4XF2|rcsb pdb:6DEC|dip:DIP-29790N	-	-	-	figure legend:Fig 1.|comment:Binding of Arp3 to cortactin was inhibited in the presence of increasing concentrations of N-WASP-VCA domain.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2012/08/07	2014/10/16	rogid:4hLyMrquWdlJnIX2pHuXqYtQgkE10090	rogid:XbkZeKzG8lGbT8dxYkGqeJT0vEg9913	intact-crc:49C45BDFE307D92F|rigid:Dd2quZk8dATTRn8wxvLSPLR6YzA	false	glutathione s tranferase tag:?-?|sufficient binding region:1-80	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:O00401	uniprotkb:P61157	intact:EBI-957615|ensembl:ENSP00000223023|uniprotkb:Q7Z746|uniprotkb:A1JUI9	intact:EBI-351419|uniprotkb:A5PJ90|uniprotkb:P32391|uniprotkb:Q08DS8|ensembl:ENSBTAP00000066597	psi-mi:wasl_human(display_long)|uniprotkb:WASL(gene name)|psi-mi:WASL(display_short)	psi-mi:arp3_bovin(display_long)|uniprotkb:ACTR3(gene name)|psi-mi:ACTR3(display_short)|uniprotkb:ARP3(gene name synonym)|uniprotkb:Actin-like protein 3(gene name synonym)|uniprotkb:Actin-2(gene name synonym)	psi-mi:"MI:0096"(pull down)	Uruno et al. (2003)	pubmed:12732638|imex:IM-18823	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1222"(mbinfo)	intact:EBI-6264493|imex:IM-18823-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0005886"(plasma membrane)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0008154"(actin polymerization or depolymerization)|go:"GO:0009617"(response to bacterium)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0016050"(vesicle organization)|go:"GO:0030027"(lamellipodium)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030048"(actin filament-based movement)|go:"GO:0030050"(vesicle transport along actin filament)|go:"GO:0030478"(actin cap)|go:"GO:0030666"(endocytic vesicle membrane)|go:"GO:0030695"(GTPase regulator activity)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032880"(regulation of protein localization)|go:"GO:0034629"|rcsb pdb:2FF3|rcsb pdb:2LNH|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|rcsb pdb:2VCP|rcsb pdb:4CC2|rcsb pdb:4CC7|reactome:R-HSA-2029482|reactome:R-HSA-203641|reactome:R-HSA-373753|reactome:R-HSA-3928662|reactome:R-HSA-418885|reactome:R-HSA-5663213|reactome:R-HSA-8856828|reactome:R-HSA-9013148|reactome:R-HSA-9013149|reactome:R-HSA-9013406|reactome:R-HSA-9013409|reactome:R-HSA-9013424|reactome:R-HSA-9664422|ensembl:ENSG00000106299(gene)|ensembl:ENST00000223023(transcript)|go:"GO:0003779"(actin binding)|go:"GO:0051301"(cell division)|go:"GO:0051491"(positive regulation of filopodium assembly)|go:"GO:0051653"(spindle localization)|go:"GO:0060997"(dendritic spine morphogenesis)|go:"GO:0065003"(protein-containing complex assembly)|go:"GO:0070062"(extracellular exosome)|go:"GO:1903526"(negative regulation of membrane tubulation)|go:"GO:2000370"(positive regulation of clathrin-dependent endocytosis)|go:"GO:2000402"(negative regulation of lymphocyte migration)|go:"GO:2000601"(positive regulation of Arp2/3 complex-mediated actin nucleation)|interpro:IPR000095(PAK-box/P21-Rho-binding)|interpro:IPR000697(EVH1)|interpro:IPR003124(Actin-binding WH2)|interpro:IPR011026(Wiscott-Aldrich syndrome, C-terminal)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR030214|interpro:IPR033927|interpro:IPR036936|mint:O00401|go:"GO:0005634"(nucleus)|refseq:NP_003932.3|dip:DIP-29042N	refseq:NP_776651.1|ensembl:ENSBTAG00000003401(gene)|ensembl:ENSBTAT00000070638(transcript)|go:"GO:0003779"(actin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005885"(Arp2/3 protein complex)|go:"GO:0010592"(positive regulation of lamellipodium assembly)|go:"GO:0034314"(Arp2/3 complex-mediated actin nucleation)|go:"GO:0035861"(site of double-strand break)|go:"GO:0042995"(cell projection)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0060271"(cilium assembly)|interpro:IPR004000(Actin/actin-like)|interpro:IPR015623(Actin-related protein 3)|interpro:IPR020902|interpro:IPR043129|rcsb pdb:1K8K|rcsb pdb:1TYQ|rcsb pdb:1U2V|rcsb pdb:2P9I|rcsb pdb:2P9K|rcsb pdb:2P9L|rcsb pdb:2P9N|rcsb pdb:2P9P|rcsb pdb:2P9S|rcsb pdb:2P9U|rcsb pdb:3DXK|rcsb pdb:3DXM|rcsb pdb:3RSE|rcsb pdb:3UKR|rcsb pdb:3UKU|rcsb pdb:3ULE|rcsb pdb:4JD2|rcsb pdb:4XEI|rcsb pdb:4XF2|rcsb pdb:6DEC|dip:DIP-29790N	-	-	-	figure legend:Fig 4A, 4B, 6A, 6B and 6C.|comment:The Arp3 immunoblot detects not the N-WASP-VCA-bound fraction from the pellet, but the unbound fraction from the supernatant. Thus, the band intensity is inversely proportional to the amount of Arp3 bound to GST-VCA.|comment:"The binding of Arp3 to N-WASP-VCA was inhibited in the presence of G-actin and increasing concentrations of cortactin. In the absence of G-actin, increasing concentrations of cortactin had no effect on the Arp3-VCA interaction (Fig 4A, 4B)."|comment:"The Arp3-N-WASP-VCA interaction was proportional to the amount of debranched actin associated with Arp3, since the presence of branched actin associated with Arp3 inhibited the interaction (Fig 6A, 6B). The presence of cortactin could not completely disrupt the interaction, since cortactin has a lower affinity for F-actin-free Arp3 than N-WASP-VCA (Fig 6C)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2012/08/07	2014/10/16	rogid:PinEPh47IRuCgiaJJZqStRhIAGQ9606	rogid:XbkZeKzG8lGbT8dxYkGqeJT0vEg9913	intact-crc:5CB34FE416379C89|rigid:7xp7WMyguuMAaznO9bPDaiq7YwA	false	glutathione s tranferase tag:?-?|sufficient binding region:392-505	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)