#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P09152 uniprotkb:P0AF26 intact:EBI-547248|uniprotkb:P78294|ensemblbacteria:AAC74308|ensemblbacteria:BAA36094 intact:EBI-555043|uniprotkb:P11351|ensemblbacteria:AAC74310|ensemblbacteria:BAA36096 psi-mi:narg_ecoli(display_long)|uniprotkb:narG(gene name)|psi-mi:narG(display_short)|uniprotkb:bisD(gene name synonym)|uniprotkb:narC(gene name synonym)|uniprotkb:b1224(locus name)|uniprotkb:Nitrate reductase A subunit alpha(gene name synonym)|uniprotkb:Quinol-nitrate oxidoreductase subunit alpha(gene name synonym)|uniprotkb:JW1215(locus name) psi-mi:narj_ecoli(display_long)|uniprotkb:narJ(gene name)|psi-mi:narJ(display_short)|uniprotkb:b1226(locus name)|uniprotkb:JW1217(locus name)|uniprotkb:Redox enzyme maturation protein NarJ(gene name synonym) psi-mi:"MI:0107"(surface plasmon resonance) Vergnes et al. (2006) pubmed:16286471|imex:IM-17279 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-3894186|imex:IM-17279-4 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_415742.1|refseq:WP_000032939.1|ensemblbacteria:AAC74308(transcript)|ensemblbacteria:b1224(gene)|ensemblbacteria:BAA36094(gene)|ensemblbacteria:BAA36094(transcript)|go:"GO:0008940"(nitrate reductase activity)|go:"GO:0009055"(electron transfer activity)|go:"GO:0009061"(anaerobic respiration)|go:"GO:0016020"(membrane)|go:"GO:0031224"(intrinsic component of membrane)|go:"GO:0031235"(intrinsic component of the cytoplasmic side of the plasma membrane)|go:"GO:0042126"(nitrate metabolic process)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0043546"(molybdopterin cofactor binding)|go:"GO:0044799"(NarGHI complex)|go:"GO:0046872"(metal ion binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR006468(Nitrate reductase, alpha subunit)|interpro:IPR006655(Molybdopterin oxidoreductase, prokaryotic, conserved site)|interpro:IPR006656(Molybdopterin oxidoreductase)|interpro:IPR006657(Molydopterin dinucleotide-binding region)|interpro:IPR006963(Molybdopterin oxidoreductase Fe4S4 region)|interpro:IPR009010(Aspartate decarboxylase-like fold)|interpro:IPR027467|interpro:IPR028189|interpro:IPR037943|rcsb pdb:1Q16|rcsb pdb:1R27|rcsb pdb:1SIW|rcsb pdb:1Y4Z|rcsb pdb:1Y5I|rcsb pdb:1Y5L|rcsb pdb:1Y5N|rcsb pdb:3EGW|rcsb pdb:3IR5|rcsb pdb:3IR6|rcsb pdb:3IR7|mint:P09152|dip:DIP-10311N refseq:NP_415744.1|refseq:WP_000571681.1|dip:DIP-35943N|ensemblbacteria:AAC74310(transcript)|ensemblbacteria:b1226(gene)|ensemblbacteria:BAA36096(gene)|ensemblbacteria:BAA36096(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0016530"(metallochaperone activity)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051131"(chaperone-mediated protein complex assembly)|interpro:IPR003765(Nitrate reductase, delta subunit)|interpro:IPR020945|interpro:IPR036411 - - crc64:17306C34A57DF1E2 figure legend:Fig 4C|comment:|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2011/05/04 2014/10/16 rogid:7OL2t6QltBEY0frD/vkRYKnIm7I83333 rogid:66OettfLGzuFMcHT2VsxrM/0RV083333 intact-crc:1DD246AE81C857A6|rigid:25f5LbBsqJPNaQaSWY1R69C855Q false tag:?-?|sufficient binding region:41-1247 his tag:?-? - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0AF26 uniprotkb:P09152 intact:EBI-555043|uniprotkb:P11351|ensemblbacteria:AAC74310|ensemblbacteria:BAA36096 intact:EBI-547248|uniprotkb:P78294|ensemblbacteria:AAC74308|ensemblbacteria:BAA36094 psi-mi:narj_ecoli(display_long)|uniprotkb:narJ(gene name)|psi-mi:narJ(display_short)|uniprotkb:b1226(locus name)|uniprotkb:JW1217(locus name)|uniprotkb:Redox enzyme maturation protein NarJ(gene name synonym) psi-mi:narg_ecoli(display_long)|uniprotkb:narG(gene name)|psi-mi:narG(display_short)|uniprotkb:bisD(gene name synonym)|uniprotkb:narC(gene name synonym)|uniprotkb:b1224(locus name)|uniprotkb:Nitrate reductase A subunit alpha(gene name synonym)|uniprotkb:Quinol-nitrate oxidoreductase subunit alpha(gene name synonym)|uniprotkb:JW1215(locus name) psi-mi:"MI:0107"(surface plasmon resonance) Vergnes et al. (2006) pubmed:16286471|imex:IM-17279 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-3894143|imex:IM-17279-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_415744.1|refseq:WP_000571681.1|dip:DIP-35943N|ensemblbacteria:AAC74310(transcript)|ensemblbacteria:b1226(gene)|ensemblbacteria:BAA36096(gene)|ensemblbacteria:BAA36096(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0016530"(metallochaperone activity)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051131"(chaperone-mediated protein complex assembly)|interpro:IPR003765(Nitrate reductase, delta subunit)|interpro:IPR020945|interpro:IPR036411 refseq:NP_415742.1|refseq:WP_000032939.1|ensemblbacteria:AAC74308(transcript)|ensemblbacteria:b1224(gene)|ensemblbacteria:BAA36094(gene)|ensemblbacteria:BAA36094(transcript)|go:"GO:0008940"(nitrate reductase activity)|go:"GO:0009055"(electron transfer activity)|go:"GO:0009061"(anaerobic respiration)|go:"GO:0016020"(membrane)|go:"GO:0031224"(intrinsic component of membrane)|go:"GO:0031235"(intrinsic component of the cytoplasmic side of the plasma membrane)|go:"GO:0042126"(nitrate metabolic process)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0043546"(molybdopterin cofactor binding)|go:"GO:0044799"(NarGHI complex)|go:"GO:0046872"(metal ion binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR006468(Nitrate reductase, alpha subunit)|interpro:IPR006655(Molybdopterin oxidoreductase, prokaryotic, conserved site)|interpro:IPR006656(Molybdopterin oxidoreductase)|interpro:IPR006657(Molydopterin dinucleotide-binding region)|interpro:IPR006963(Molybdopterin oxidoreductase Fe4S4 region)|interpro:IPR009010(Aspartate decarboxylase-like fold)|interpro:IPR027467|interpro:IPR028189|interpro:IPR037943|rcsb pdb:1Q16|rcsb pdb:1R27|rcsb pdb:1SIW|rcsb pdb:1Y4Z|rcsb pdb:1Y5I|rcsb pdb:1Y5L|rcsb pdb:1Y5N|rcsb pdb:3EGW|rcsb pdb:3IR5|rcsb pdb:3IR6|rcsb pdb:3IR7|mint:P09152|dip:DIP-10311N - crc64:17306C34A57DF1E2 - figure legend:Fig 4B|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2011/05/04 2014/10/16 rogid:66OettfLGzuFMcHT2VsxrM/0RV083333 rogid:7OL2t6QltBEY0frD/vkRYKnIm7I83333 intact-crc:80D7DD46110B83C5|rigid:25f5LbBsqJPNaQaSWY1R69C855Q false his tag:?-? tag:?-?|sufficient binding region:1-40 - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P09152 uniprotkb:P11349 intact:EBI-547248|uniprotkb:P78294|ensemblbacteria:AAC74308|ensemblbacteria:BAA36094 intact:EBI-555067|ensemblbacteria:AAC74309|ensemblbacteria:BAA36095 psi-mi:narg_ecoli(display_long)|uniprotkb:narG(gene name)|psi-mi:narG(display_short)|uniprotkb:bisD(gene name synonym)|uniprotkb:narC(gene name synonym)|uniprotkb:b1224(locus name)|uniprotkb:Nitrate reductase A subunit alpha(gene name synonym)|uniprotkb:Quinol-nitrate oxidoreductase subunit alpha(gene name synonym)|uniprotkb:JW1215(locus name) psi-mi:narh_ecoli(display_long)|uniprotkb:narH(gene name)|psi-mi:narH(display_short)|uniprotkb:b1225(locus name)|uniprotkb:Nitrate reductase A subunit beta(gene name synonym)|uniprotkb:Quinol-nitrate oxidoreductase subunit beta(gene name synonym)|uniprotkb:JW1216(locus name) psi-mi:"MI:0018"(two hybrid) Vergnes et al. (2006) pubmed:16286471|imex:IM-17279 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-3893771|imex:IM-17279-2 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_415742.1|refseq:WP_000032939.1|ensemblbacteria:AAC74308(transcript)|ensemblbacteria:b1224(gene)|ensemblbacteria:BAA36094(gene)|ensemblbacteria:BAA36094(transcript)|go:"GO:0008940"(nitrate reductase activity)|go:"GO:0009055"(electron transfer activity)|go:"GO:0009061"(anaerobic respiration)|go:"GO:0016020"(membrane)|go:"GO:0031224"(intrinsic component of membrane)|go:"GO:0031235"(intrinsic component of the cytoplasmic side of the plasma membrane)|go:"GO:0042126"(nitrate metabolic process)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0043546"(molybdopterin cofactor binding)|go:"GO:0044799"(NarGHI complex)|go:"GO:0046872"(metal ion binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR006468(Nitrate reductase, alpha subunit)|interpro:IPR006655(Molybdopterin oxidoreductase, prokaryotic, conserved site)|interpro:IPR006656(Molybdopterin oxidoreductase)|interpro:IPR006657(Molydopterin dinucleotide-binding region)|interpro:IPR006963(Molybdopterin oxidoreductase Fe4S4 region)|interpro:IPR009010(Aspartate decarboxylase-like fold)|interpro:IPR027467|interpro:IPR028189|interpro:IPR037943|rcsb pdb:1Q16|rcsb pdb:1R27|rcsb pdb:1SIW|rcsb pdb:1Y4Z|rcsb pdb:1Y5I|rcsb pdb:1Y5L|rcsb pdb:1Y5N|rcsb pdb:3EGW|rcsb pdb:3IR5|rcsb pdb:3IR6|rcsb pdb:3IR7|mint:P09152|dip:DIP-10311N refseq:NP_415743.1|refseq:WP_000702650.1|dip:DIP-10312N|ensemblbacteria:AAC74309(transcript)|ensemblbacteria:b1225(gene)|ensemblbacteria:BAA36095(gene)|ensemblbacteria:BAA36095(transcript)|go:"GO:0008940"(nitrate reductase activity)|go:"GO:0009055"(electron transfer activity)|go:"GO:0009061"(anaerobic respiration)|go:"GO:0016020"(membrane)|go:"GO:0031224"(intrinsic component of membrane)|go:"GO:0031235"(intrinsic component of the cytoplasmic side of the plasma membrane)|go:"GO:0042126"(nitrate metabolic process)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0044799"(NarGHI complex)|go:"GO:0046872"(metal ion binding)|go:"GO:0051538"(3 iron, 4 sulfur cluster binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR006547(Nitrate reductase, beta subunit)|interpro:IPR017896|interpro:IPR029263|interpro:IPR038262|rcsb pdb:1Q16|rcsb pdb:1R27|rcsb pdb:1SIW|rcsb pdb:1Y4Z|rcsb pdb:1Y5I|rcsb pdb:1Y5L|rcsb pdb:1Y5N|rcsb pdb:3EGW|rcsb pdb:3IR5|rcsb pdb:3IR6|rcsb pdb:3IR7 - - - figure legend:Fig 4A|comment:"NarG (aa 1-40) binds NarH, but very weakly compared to the NArg(1-40) NarJ reaction. Similarly the NarG construct (aa 41-1247) also binds to NarH, but by a slightly reduced amount."|caution:This interaction gave a result of 45 Miller units as compared to the negative control that gave a result of 50 Miller units.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2011/05/04 2014/10/16 rogid:7OL2t6QltBEY0frD/vkRYKnIm7I83333 rogid:m5XqVd9BJ0Ll1MyC6wLgB6Lla8o83333 intact-crc:8D06DA427BA1352A|rigid:c5LQmb7UEWs867ZP20VXrm+HGyg false tag:?-?|sufficient binding region:1-40 - - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P09152 uniprotkb:P0AF26 intact:EBI-547248|uniprotkb:P78294|ensemblbacteria:AAC74308|ensemblbacteria:BAA36094 intact:EBI-555043|uniprotkb:P11351|ensemblbacteria:AAC74310|ensemblbacteria:BAA36096 psi-mi:narg_ecoli(display_long)|uniprotkb:narG(gene name)|psi-mi:narG(display_short)|uniprotkb:bisD(gene name synonym)|uniprotkb:narC(gene name synonym)|uniprotkb:b1224(locus name)|uniprotkb:Nitrate reductase A subunit alpha(gene name synonym)|uniprotkb:Quinol-nitrate oxidoreductase subunit alpha(gene name synonym)|uniprotkb:JW1215(locus name) psi-mi:narj_ecoli(display_long)|uniprotkb:narJ(gene name)|psi-mi:narJ(display_short)|uniprotkb:b1226(locus name)|uniprotkb:JW1217(locus name)|uniprotkb:Redox enzyme maturation protein NarJ(gene name synonym) psi-mi:"MI:0018"(two hybrid) Vergnes et al. (2006) pubmed:16286471|imex:IM-17279 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-3893728|imex:IM-17279-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_415742.1|refseq:WP_000032939.1|ensemblbacteria:AAC74308(transcript)|ensemblbacteria:b1224(gene)|ensemblbacteria:BAA36094(gene)|ensemblbacteria:BAA36094(transcript)|go:"GO:0008940"(nitrate reductase activity)|go:"GO:0009055"(electron transfer activity)|go:"GO:0009061"(anaerobic respiration)|go:"GO:0016020"(membrane)|go:"GO:0031224"(intrinsic component of membrane)|go:"GO:0031235"(intrinsic component of the cytoplasmic side of the plasma membrane)|go:"GO:0042126"(nitrate metabolic process)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0043546"(molybdopterin cofactor binding)|go:"GO:0044799"(NarGHI complex)|go:"GO:0046872"(metal ion binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR006468(Nitrate reductase, alpha subunit)|interpro:IPR006655(Molybdopterin oxidoreductase, prokaryotic, conserved site)|interpro:IPR006656(Molybdopterin oxidoreductase)|interpro:IPR006657(Molydopterin dinucleotide-binding region)|interpro:IPR006963(Molybdopterin oxidoreductase Fe4S4 region)|interpro:IPR009010(Aspartate decarboxylase-like fold)|interpro:IPR027467|interpro:IPR028189|interpro:IPR037943|rcsb pdb:1Q16|rcsb pdb:1R27|rcsb pdb:1SIW|rcsb pdb:1Y4Z|rcsb pdb:1Y5I|rcsb pdb:1Y5L|rcsb pdb:1Y5N|rcsb pdb:3EGW|rcsb pdb:3IR5|rcsb pdb:3IR6|rcsb pdb:3IR7|mint:P09152|dip:DIP-10311N refseq:NP_415744.1|refseq:WP_000571681.1|dip:DIP-35943N|ensemblbacteria:AAC74310(transcript)|ensemblbacteria:b1226(gene)|ensemblbacteria:BAA36096(gene)|ensemblbacteria:BAA36096(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0016530"(metallochaperone activity)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051131"(chaperone-mediated protein complex assembly)|interpro:IPR003765(Nitrate reductase, delta subunit)|interpro:IPR020945|interpro:IPR036411 - - crc64:17306C34A57DF1E2 figure legend:Fig 4A|comment:Unexpectedly, deletion of the first 40 residues of NarG does not hamper its interaction with NarJ.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2011/05/04 2014/10/16 rogid:7OL2t6QltBEY0frD/vkRYKnIm7I83333 rogid:66OettfLGzuFMcHT2VsxrM/0RV083333 intact-crc:5E5F3EB41D4F5564|rigid:25f5LbBsqJPNaQaSWY1R69C855Q false tag:?-?|sufficient binding region:1-40 - - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant)