#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:Q12380 uniprotkb:P38316 intact:EBI-2664|uniprotkb:D6W3M0|ensemblfungi:YPL149W intact:EBI-2692|uniprotkb:D6VQL3|ensemblfungi:YBR217W psi-mi:atg5_yeast(display_long)|uniprotkb:ATG5(gene name)|psi-mi:ATG5(display_short)|uniprotkb:APG5(gene name synonym)|uniprotkb:YPL149W(locus name)|uniprotkb:P2601(orf name) psi-mi:atg12_yeast(display_long)|uniprotkb:ATG12(gene name)|psi-mi:ATG12(display_short)|uniprotkb:APG12(gene name synonym)|uniprotkb:YBR217W(locus name)|uniprotkb:YBR1506(orf name)|uniprotkb:Autophagy-related protein 12(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Mizushima et al. (1998) pubmed:9759731|imex:IM-19220 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2639387|imex:IM-19220-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_015176.1|sgd:S000006070|reactome:R-SCE-1632852|dip:DIP-1195N|reactome:R-SCE-8934903|ensemblfungi:YPL149W(gene)|ensemblfungi:YPL149W_mRNA(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0000407"(phagophore assembly site)|go:"GO:0000422"(autophagy of mitochondrion)|go:"GO:0005634"(nucleus)|go:"GO:0005776"(autophagosome)|go:"GO:0005829"(cytosol)|go:"GO:0006501"(C-terminal protein lipidation)|go:"GO:0006995"(cellular response to nitrogen starvation)|go:"GO:0008047"(enzyme activator activity)|go:"GO:0016020"(membrane)|go:"GO:0016236"(macroautophagy)|go:"GO:0016787"(hydrolase activity)|go:"GO:0032258"(cytoplasm to vacuole transport by the Cvt pathway)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0034274"(Atg12-Atg5-Atg16 complex)|go:"GO:0034727"(piecemeal microautophagy of the nucleus)|go:"GO:0044804"(autophagy of nucleus)|go:"GO:0044805"(late nucleophagy)|go:"GO:0061908"(phagophore)|go:"GO:0061912"(selective autophagy)|go:"GO:0120095"(vacuole-isolation membrane contact site)|go:"GO:0140355"(cargo receptor ligand activity)|interpro:IPR007239(Autophagy protein 5)|interpro:IPR042526|interpro:IPR042527|rcsb pdb:2DYM|rcsb pdb:2DYO|rcsb pdb:3W1S refseq:NP_009776.1|sgd:S000000421|dip:DIP-1189N|reactome:R-SCE-1632852|reactome:R-SCE-8934903|ensemblfungi:YBR217W(gene)|ensemblfungi:YBR217W_mRNA(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0000407"(phagophore assembly site)|go:"GO:0000422"(autophagy of mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006501"(C-terminal protein lipidation)|go:"GO:0008047"(enzyme activator activity)|go:"GO:0031386"(protein tag)|go:"GO:0032258"(cytoplasm to vacuole transport by the Cvt pathway)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0034274"(Atg12-Atg5-Atg16 complex)|go:"GO:0034727"(piecemeal microautophagy of the nucleus)|go:"GO:0044804"(autophagy of nucleus)|go:"GO:0044805"(late nucleophagy)|go:"GO:0061739"(protein lipidation involved in autophagosome assembly)|interpro:IPR007242(Autophagy protein 12)|interpro:IPR029071|rcsb pdb:3W1S - crc64:BE513A631DEAA0A1 crc64:74B3C9FD3CEA9265 figure legend:"Immunoprecipitation analysis revealed that the 70K band included both Apg5 and Apg12 (Fig. 2d)."|figure legend:Fig. 2D|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2010/04/12 2014/10/16 rogid:PMSbzCApcY0S518yzmdDm3bPY74559292 rogid:2hGhAejWvm9rPoB9m8JPmjSjFIo559292 intact-crc:9C451F3A3F9F4217|rigid:5o01wZeP75UIqtwvA+gVr90lQNI false ha tag:?-? myc tag:?-? - - psi-mi:"MI:0705"(anti tag western blot) psi-mi:"MI:0705"(anti tag western blot) uniprotkb:Q12380 uniprotkb:P38316 intact:EBI-2664|uniprotkb:D6W3M0|ensemblfungi:YPL149W intact:EBI-2692|uniprotkb:D6VQL3|ensemblfungi:YBR217W psi-mi:atg5_yeast(display_long)|uniprotkb:ATG5(gene name)|psi-mi:ATG5(display_short)|uniprotkb:APG5(gene name synonym)|uniprotkb:YPL149W(locus name)|uniprotkb:P2601(orf name) psi-mi:atg12_yeast(display_long)|uniprotkb:ATG12(gene name)|psi-mi:ATG12(display_short)|uniprotkb:APG12(gene name synonym)|uniprotkb:YBR217W(locus name)|uniprotkb:YBR1506(orf name)|uniprotkb:Autophagy-related protein 12(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Mizushima et al. (1998) pubmed:9759731|imex:IM-19220 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2639430|imex:IM-19220-2 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_015176.1|sgd:S000006070|reactome:R-SCE-1632852|dip:DIP-1195N|reactome:R-SCE-8934903|ensemblfungi:YPL149W(gene)|ensemblfungi:YPL149W_mRNA(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0000407"(phagophore assembly site)|go:"GO:0000422"(autophagy of mitochondrion)|go:"GO:0005634"(nucleus)|go:"GO:0005776"(autophagosome)|go:"GO:0005829"(cytosol)|go:"GO:0006501"(C-terminal protein lipidation)|go:"GO:0006995"(cellular response to nitrogen starvation)|go:"GO:0008047"(enzyme activator activity)|go:"GO:0016020"(membrane)|go:"GO:0016236"(macroautophagy)|go:"GO:0016787"(hydrolase activity)|go:"GO:0032258"(cytoplasm to vacuole transport by the Cvt pathway)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0034274"(Atg12-Atg5-Atg16 complex)|go:"GO:0034727"(piecemeal microautophagy of the nucleus)|go:"GO:0044804"(autophagy of nucleus)|go:"GO:0044805"(late nucleophagy)|go:"GO:0061908"(phagophore)|go:"GO:0061912"(selective autophagy)|go:"GO:0120095"(vacuole-isolation membrane contact site)|go:"GO:0140355"(cargo receptor ligand activity)|interpro:IPR007239(Autophagy protein 5)|interpro:IPR042526|interpro:IPR042527|rcsb pdb:2DYM|rcsb pdb:2DYO|rcsb pdb:3W1S refseq:NP_009776.1|sgd:S000000421|dip:DIP-1189N|reactome:R-SCE-1632852|reactome:R-SCE-8934903|ensemblfungi:YBR217W(gene)|ensemblfungi:YBR217W_mRNA(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0000407"(phagophore assembly site)|go:"GO:0000422"(autophagy of mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006501"(C-terminal protein lipidation)|go:"GO:0008047"(enzyme activator activity)|go:"GO:0031386"(protein tag)|go:"GO:0032258"(cytoplasm to vacuole transport by the Cvt pathway)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0034274"(Atg12-Atg5-Atg16 complex)|go:"GO:0034727"(piecemeal microautophagy of the nucleus)|go:"GO:0044804"(autophagy of nucleus)|go:"GO:0044805"(late nucleophagy)|go:"GO:0061739"(protein lipidation involved in autophagosome assembly)|interpro:IPR007242(Autophagy protein 12)|interpro:IPR029071|rcsb pdb:3W1S - crc64:BE513A631DEAA0A1 crc64:74B3C9FD3CEA9265 figure legend:"We therefore examined their intracellular localization by sucrose density-gradient centrifugation analysis and found that free Apg5 and the Apg5/Apg12 conjugate co-fractionated (Fig. 6); in contrast, most of the Apg12 was in the denser fractions."|figure legend:Fig. 6B|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2010/04/12 2014/10/16 rogid:PMSbzCApcY0S518yzmdDm3bPY74559292 rogid:2hGhAejWvm9rPoB9m8JPmjSjFIo559292 intact-crc:B23656FCD1D9991C|rigid:5o01wZeP75UIqtwvA+gVr90lQNI false ha tag:?-? ha tag:?-? - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot)