#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:Q9CQY1 uniprotkb:Q9CPX6 intact:EBI-2911788|uniprotkb:Q3TKE5|uniprotkb:Q9D7Y5|ensembl:ENSMUSP00000038489 intact:EBI-2911810|ensembl:ENSMUSP00000023343|uniprotkb:Q3TXJ9 psi-mi:atg12_mouse(display_long)|uniprotkb:Atg12(gene name)|psi-mi:Atg12(display_short)|uniprotkb:Apg12(gene name synonym)|uniprotkb:Apg12l(gene name synonym)|uniprotkb:Autophagy-related protein 12(gene name synonym) psi-mi:atg3_mouse(display_long)|uniprotkb:Atg3(gene name)|psi-mi:Atg3(display_short)|uniprotkb:Apg3l(gene name synonym)|uniprotkb:Autophagy-related protein 3(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Radoshevich et al. (2010) imex:IM-14975|pubmed:20723759 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2911804|imex:IM-14975-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0000045"(autophagosome assembly)|go:"GO:0000422"(autophagy of mitochondrion)|go:"GO:0005776"(autophagosome)|go:"GO:0005829"(cytosol)|go:"GO:0006501"(C-terminal protein lipidation)|go:"GO:0006914"(autophagy)|go:"GO:0016020"(membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0034274"(Atg12-Atg5-Atg16 complex)|go:"GO:0044804"(autophagy of nucleus)|interpro:IPR007242(Autophagy protein 12)|interpro:IPR029071|mint:Q9CQY1|reactome:R-MMU-1632852|reactome:R-MMU-5205685|reactome:R-MMU-8934903|ensembl:ENSMUSG00000032905(gene)|ensembl:ENSMUST00000035648(transcript)|refseq:NP_080493.2|dip:DIP-57729N ensembl:ENSMUSG00000022663(gene)|ensembl:ENSMUST00000023343(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0000153"(cytoplasmic ubiquitin ligase complex)|go:"GO:0000422"(autophagy of mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0015031"(protein transport)|go:"GO:0016236"(macroautophagy)|go:"GO:0019776"(Atg8 ligase activity)|go:"GO:0019777"(Atg12 transferase activity)|go:"GO:0019787"(ubiquitin-like protein transferase activity)|go:"GO:0019899"(enzyme binding)|go:"GO:0043653"(mitochondrial fragmentation involved in apoptotic process)|go:"GO:0044804"(autophagy of nucleus)|go:"GO:0050765"(negative regulation of phagocytosis)|go:"GO:1902017"(regulation of cilium assembly)|interpro:IPR007135(Autophagocytosis associated protein, C-terminal)|mint:Q9CPX6|reactome:R-MMU-1632852|go:"GO:0016567"(protein ubiquitination)|refseq:NP_080678.1|dip:DIP-60109N psi-mi:"MI:0469"(intact) - - figure legend:Fig. 2A|comment:Interaction observed in atg5-/- cells|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-embryonic_fibroblast)|taxid:10090(Mus musculus embryonic fibroblasts) - 2010/09/02 2014/10/16 rogid:VPDzzrLDhtPg3TwA3Qbhr5mB0CQ10090 rogid:VW5Xh5I7A5K+xLjAfSmLLN4cftw10090 intact-crc:43E253900D5A1ED6|rigid:S2w9uhkTEDVTcvFCISSjsuldoE8 false flag tag:n-n|ha tag:n-n|necessary binding region:141-141 - - - psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:Q9CQY1 uniprotkb:Q9CPX6 intact:EBI-2911788|uniprotkb:Q3TKE5|uniprotkb:Q9D7Y5|ensembl:ENSMUSP00000038489 intact:EBI-2911810|ensembl:ENSMUSP00000023343|uniprotkb:Q3TXJ9 psi-mi:atg12_mouse(display_long)|uniprotkb:Atg12(gene name)|psi-mi:Atg12(display_short)|uniprotkb:Apg12(gene name synonym)|uniprotkb:Apg12l(gene name synonym)|uniprotkb:Autophagy-related protein 12(gene name synonym) psi-mi:atg3_mouse(display_long)|uniprotkb:Atg3(gene name)|psi-mi:Atg3(display_short)|uniprotkb:Apg3l(gene name synonym)|uniprotkb:Autophagy-related protein 3(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Radoshevich et al. (2010) imex:IM-14975|pubmed:20723759 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2911835|imex:IM-14975-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0000045"(autophagosome assembly)|go:"GO:0000422"(autophagy of mitochondrion)|go:"GO:0005776"(autophagosome)|go:"GO:0005829"(cytosol)|go:"GO:0006501"(C-terminal protein lipidation)|go:"GO:0006914"(autophagy)|go:"GO:0016020"(membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0034274"(Atg12-Atg5-Atg16 complex)|go:"GO:0044804"(autophagy of nucleus)|interpro:IPR007242(Autophagy protein 12)|interpro:IPR029071|mint:Q9CQY1|reactome:R-MMU-1632852|reactome:R-MMU-5205685|reactome:R-MMU-8934903|ensembl:ENSMUSG00000032905(gene)|ensembl:ENSMUST00000035648(transcript)|refseq:NP_080493.2|dip:DIP-57729N ensembl:ENSMUSG00000022663(gene)|ensembl:ENSMUST00000023343(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0000153"(cytoplasmic ubiquitin ligase complex)|go:"GO:0000422"(autophagy of mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0015031"(protein transport)|go:"GO:0016236"(macroautophagy)|go:"GO:0019776"(Atg8 ligase activity)|go:"GO:0019777"(Atg12 transferase activity)|go:"GO:0019787"(ubiquitin-like protein transferase activity)|go:"GO:0019899"(enzyme binding)|go:"GO:0043653"(mitochondrial fragmentation involved in apoptotic process)|go:"GO:0044804"(autophagy of nucleus)|go:"GO:0050765"(negative regulation of phagocytosis)|go:"GO:1902017"(regulation of cilium assembly)|interpro:IPR007135(Autophagocytosis associated protein, C-terminal)|mint:Q9CPX6|reactome:R-MMU-1632852|go:"GO:0016567"(protein ubiquitination)|refseq:NP_080678.1|dip:DIP-60109N psi-mi:"MI:0469"(intact) - - figure legend:Fig. 2B|comment:Interaction observed in atg5-/- cells|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-embryonic_fibroblast)|taxid:10090(Mus musculus embryonic fibroblasts) - 2010/09/02 2014/10/16 rogid:VPDzzrLDhtPg3TwA3Qbhr5mB0CQ10090 rogid:VW5Xh5I7A5K+xLjAfSmLLN4cftw10090 intact-crc:1D742601CE176ED5|rigid:S2w9uhkTEDVTcvFCISSjsuldoE8 false flag tag:n-n|ha tag:n-n|necessary binding region:141-141 - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q9CQY1 uniprotkb:Q99J83 intact:EBI-2911788|uniprotkb:Q3TKE5|uniprotkb:Q9D7Y5|ensembl:ENSMUSP00000038489 intact:EBI-2911848|ensembl:ENSMUSP00000044769 psi-mi:atg12_mouse(display_long)|uniprotkb:Atg12(gene name)|psi-mi:Atg12(display_short)|uniprotkb:Apg12(gene name synonym)|uniprotkb:Apg12l(gene name synonym)|uniprotkb:Autophagy-related protein 12(gene name synonym) psi-mi:atg5_mouse(display_long)|uniprotkb:Atg5(gene name)|psi-mi:Atg5(display_short)|uniprotkb:Apg5l(gene name synonym)|uniprotkb:APG5-like(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Radoshevich et al. (2010) imex:IM-14975|pubmed:20723759 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2911870|imex:IM-14975-2 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0000045"(autophagosome assembly)|go:"GO:0000422"(autophagy of mitochondrion)|go:"GO:0005776"(autophagosome)|go:"GO:0005829"(cytosol)|go:"GO:0006501"(C-terminal protein lipidation)|go:"GO:0006914"(autophagy)|go:"GO:0016020"(membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0034274"(Atg12-Atg5-Atg16 complex)|go:"GO:0044804"(autophagy of nucleus)|interpro:IPR007242(Autophagy protein 12)|interpro:IPR029071|mint:Q9CQY1|reactome:R-MMU-1632852|reactome:R-MMU-5205685|reactome:R-MMU-8934903|ensembl:ENSMUSG00000032905(gene)|ensembl:ENSMUST00000035648(transcript)|refseq:NP_080493.2|dip:DIP-57729N ensembl:ENSMUSG00000038160(gene)|ensembl:ENSMUST00000039286(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0000422"(autophagy of mitochondrion)|go:"GO:0001974"(blood vessel remodeling)|go:"GO:0002718"(regulation of cytokine production involved in immune response)|go:"GO:0005737"(cytoplasm)|go:"GO:0005776"(autophagosome)|go:"GO:0005829"(cytosol)|go:"GO:0005930"(axoneme)|go:"GO:0006501"(C-terminal protein lipidation)|go:"GO:0006914"(autophagy)|go:"GO:0006915"(apoptotic process)|go:"GO:0006995"(cellular response to nitrogen starvation)|go:"GO:0009267"(cellular response to starvation)|go:"GO:0016020"(membrane)|go:"GO:0016236"(macroautophagy)|go:"GO:0019883"(antigen processing and presentation of endogenous antigen)|go:"GO:0031397"(negative regulation of protein ubiquitination)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0009620"(response to fungus)|go:"GO:0034274"(Atg12-Atg5-Atg16 complex)|go:"GO:0035973"(aggrephagy)|go:"GO:0039689"(negative stranded viral RNA replication)|go:"GO:0042311"(vasodilation)|go:"GO:0042493"|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043687"(post-translational protein modification)|go:"GO:0044233"(mitochondria-associated endoplasmic reticulum membrane)|go:"GO:0044804"(autophagy of nucleus)|go:"GO:0045060"(negative thymic T cell selection)|go:"GO:0048840"(otolith development)|go:"GO:0050765"(negative regulation of phagocytosis)|go:"GO:0051279"(regulation of release of sequestered calcium ion into cytosol)|go:"GO:0055015"(ventricular cardiac muscle cell development)|go:"GO:0060047"(heart contraction)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0061739"(protein lipidation involved in autophagosome assembly)|go:"GO:0070257"(positive regulation of mucus secretion)|go:"GO:0071500"(cellular response to nitrosative stress)|go:"GO:0075044"(positive regulation by symbiont of host autophagy)|go:"GO:1902017"(regulation of cilium assembly)|go:"GO:2000377"(regulation of reactive oxygen species metabolic process)|go:"GO:2000378"(negative regulation of reactive oxygen species metabolic process)|go:"GO:2000619"(negative regulation of histone H4-K16 acetylation)|interpro:IPR007239(Autophagy protein 5)|interpro:IPR042526|interpro:IPR042527|mint:Q99J83|reactome:R-MMU-1632852|reactome:R-MMU-5205685|reactome:R-MMU-8934903|refseq:XP_011241410.1|refseq:NP_444299.1|refseq:NP_001300942.1|dip:DIP-46420N psi-mi:"MI:0469"(intact) - - figure legend:Fig. 1F|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-embryonic_fibroblast)|taxid:10090(Mus musculus embryonic fibroblasts) - 2010/09/02 2014/10/16 rogid:VPDzzrLDhtPg3TwA3Qbhr5mB0CQ10090 rogid:XNYb541lnGe1mjdDHei6DNhiNfk10090 intact-crc:A00E7D74A7354C00|rigid:BPAhBzVXn0Di8L74znt+z+878Q4 false flag tag:n-n|ha tag:n-n|necessary binding region:141-141 - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q9CQY1 uniprotkb:Q9NT62 intact:EBI-2911788|uniprotkb:Q3TKE5|uniprotkb:Q9D7Y5|ensembl:ENSMUSP00000038489 intact:EBI-988094|uniprotkb:Q6PKC5|uniprotkb:Q9H6L9|ensembl:ENSP00000283290 psi-mi:atg12_mouse(display_long)|uniprotkb:Atg12(gene name)|psi-mi:Atg12(display_short)|uniprotkb:Apg12(gene name synonym)|uniprotkb:Apg12l(gene name synonym)|uniprotkb:Autophagy-related protein 12(gene name synonym) psi-mi:atg3_human(display_long)|uniprotkb:Autophagy-related protein 3(gene name synonym)|uniprotkb:Protein PC3-96(gene name synonym)|uniprotkb:ATG3(gene name)|psi-mi:ATG3(display_short)|uniprotkb:APG3(gene name synonym)|uniprotkb:APG3L(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Radoshevich et al. (2010) imex:IM-14975|pubmed:20723759 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2911889|imex:IM-14975-5 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) go:"GO:0000045"(autophagosome assembly)|go:"GO:0000422"(autophagy of mitochondrion)|go:"GO:0005776"(autophagosome)|go:"GO:0005829"(cytosol)|go:"GO:0006501"(C-terminal protein lipidation)|go:"GO:0006914"(autophagy)|go:"GO:0016020"(membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0034274"(Atg12-Atg5-Atg16 complex)|go:"GO:0044804"(autophagy of nucleus)|interpro:IPR007242(Autophagy protein 12)|interpro:IPR029071|mint:Q9CQY1|reactome:R-MMU-1632852|reactome:R-MMU-5205685|reactome:R-MMU-8934903|ensembl:ENSMUSG00000032905(gene)|ensembl:ENSMUST00000035648(transcript)|refseq:NP_080493.2|dip:DIP-57729N refseq:NP_071933.2|refseq:NP_001265641.1|dip:DIP-35052N|ensembl:ENSG00000144848(gene)|ensembl:ENST00000283290(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0000153"(cytoplasmic ubiquitin ligase complex)|go:"GO:0000422"(autophagy of mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0006612"(protein targeting to membrane)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0019776"(Atg8 ligase activity)|go:"GO:0019777"(Atg12 transferase activity)|go:"GO:0019787"(ubiquitin-like protein transferase activity)|go:"GO:0019899"(enzyme binding)|go:"GO:0043653"(mitochondrial fragmentation involved in apoptotic process)|go:"GO:0044804"(autophagy of nucleus)|go:"GO:0050765"(negative regulation of phagocytosis)|go:"GO:1902017"(regulation of cilium assembly)|interpro:IPR007135(Autophagocytosis associated protein, C-terminal)|rcsb pdb:4NAW|reactome:R-HSA-1632852 psi-mi:"MI:0469"(intact) - - figure legend:Supp. Fig. 1|comment:An ATG12-ATG3 doublet collapsed to a single protein when treated with calf intestinal phosphatase, suggesting that the ATG12-ATG3 complex undergoes phosphorylation.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-mcf10a)|taxid:9606(Human non-transformed breast epithelial cell line) - 2010/09/02 2014/10/16 rogid:VPDzzrLDhtPg3TwA3Qbhr5mB0CQ10090 rogid:yKaFEtGlnMydgWQ1aqZJOGHtKRI9606 intact-crc:584A325B2D73443A|rigid:K/W3fzved1wP4D5mf3U7myo4BNY false flag tag:n-n|ha tag:n-n|necessary binding region:141-141 - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q9CPX6 uniprotkb:Q9CQY1 intact:EBI-2911810|ensembl:ENSMUSP00000023343|uniprotkb:Q3TXJ9 intact:EBI-2911788|uniprotkb:Q3TKE5|uniprotkb:Q9D7Y5|ensembl:ENSMUSP00000038489 psi-mi:atg3_mouse(display_long)|uniprotkb:Atg3(gene name)|psi-mi:Atg3(display_short)|uniprotkb:Apg3l(gene name synonym)|uniprotkb:Autophagy-related protein 3(gene name synonym) psi-mi:atg12_mouse(display_long)|uniprotkb:Atg12(gene name)|psi-mi:Atg12(display_short)|uniprotkb:Apg12(gene name synonym)|uniprotkb:Apg12l(gene name synonym)|uniprotkb:Autophagy-related protein 12(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Radoshevich et al. (2010) imex:IM-14975|pubmed:20723759 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2911907|imex:IM-14975-4 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSMUSG00000022663(gene)|ensembl:ENSMUST00000023343(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0000153"(cytoplasmic ubiquitin ligase complex)|go:"GO:0000422"(autophagy of mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0015031"(protein transport)|go:"GO:0016236"(macroautophagy)|go:"GO:0019776"(Atg8 ligase activity)|go:"GO:0019777"(Atg12 transferase activity)|go:"GO:0019787"(ubiquitin-like protein transferase activity)|go:"GO:0019899"(enzyme binding)|go:"GO:0043653"(mitochondrial fragmentation involved in apoptotic process)|go:"GO:0044804"(autophagy of nucleus)|go:"GO:0050765"(negative regulation of phagocytosis)|go:"GO:1902017"(regulation of cilium assembly)|interpro:IPR007135(Autophagocytosis associated protein, C-terminal)|mint:Q9CPX6|reactome:R-MMU-1632852|go:"GO:0016567"(protein ubiquitination)|refseq:NP_080678.1|dip:DIP-60109N go:"GO:0000045"(autophagosome assembly)|go:"GO:0000422"(autophagy of mitochondrion)|go:"GO:0005776"(autophagosome)|go:"GO:0005829"(cytosol)|go:"GO:0006501"(C-terminal protein lipidation)|go:"GO:0006914"(autophagy)|go:"GO:0016020"(membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0034274"(Atg12-Atg5-Atg16 complex)|go:"GO:0044804"(autophagy of nucleus)|interpro:IPR007242(Autophagy protein 12)|interpro:IPR029071|mint:Q9CQY1|reactome:R-MMU-1632852|reactome:R-MMU-5205685|reactome:R-MMU-8934903|ensembl:ENSMUSG00000032905(gene)|ensembl:ENSMUST00000035648(transcript)|refseq:NP_080493.2|dip:DIP-57729N psi-mi:"MI:0469"(intact) - - figure legend:Fig. 1F|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-embryonic_fibroblast)|taxid:10090(Mus musculus embryonic fibroblasts) - 2010/09/02 2014/10/16 rogid:VW5Xh5I7A5K+xLjAfSmLLN4cftw10090 rogid:VPDzzrLDhtPg3TwA3Qbhr5mB0CQ10090 intact-crc:284ED290F54F6737|rigid:S2w9uhkTEDVTcvFCISSjsuldoE8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot)