#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P0AF26	uniprotkb:P09152	intact:EBI-555043|uniprotkb:P11351|ensemblbacteria:AAC74310|ensemblbacteria:BAA36096	intact:EBI-547248|uniprotkb:P78294|ensemblbacteria:AAC74308|ensemblbacteria:BAA36094	psi-mi:narj_ecoli(display_long)|uniprotkb:narJ(gene name)|psi-mi:narJ(display_short)|uniprotkb:b1226(locus name)|uniprotkb:JW1217(locus name)|uniprotkb:Redox enzyme maturation protein NarJ(gene name synonym)	psi-mi:narg_ecoli(display_long)|uniprotkb:narG(gene name)|psi-mi:narG(display_short)|uniprotkb:bisD(gene name synonym)|uniprotkb:narC(gene name synonym)|uniprotkb:b1224(locus name)|uniprotkb:Nitrate reductase A subunit alpha(gene name synonym)|uniprotkb:Quinol-nitrate oxidoreductase subunit alpha(gene name synonym)|uniprotkb:JW1215(locus name)	psi-mi:"MI:0077"(nuclear magnetic resonance)	Zakian et al. (2010)	pubmed:20236317|imex:IM-15683|mint:MINT-7557431	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-3893033|imex:IM-15683-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_415744.1|refseq:WP_000571681.1|dip:DIP-35943N|ensemblbacteria:AAC74310(transcript)|ensemblbacteria:b1226(gene)|ensemblbacteria:BAA36096(gene)|ensemblbacteria:BAA36096(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0016530"(metallochaperone activity)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051131"(chaperone-mediated protein complex assembly)|interpro:IPR003765(Nitrate reductase, delta subunit)|interpro:IPR020945|interpro:IPR036411	refseq:NP_415742.1|refseq:WP_000032939.1|ensemblbacteria:AAC74308(transcript)|ensemblbacteria:b1224(gene)|ensemblbacteria:BAA36094(gene)|ensemblbacteria:BAA36094(transcript)|go:"GO:0008940"(nitrate reductase activity)|go:"GO:0009055"(electron transfer activity)|go:"GO:0009061"(anaerobic respiration)|go:"GO:0016020"(membrane)|go:"GO:0031224"(intrinsic component of membrane)|go:"GO:0031235"(intrinsic component of the cytoplasmic side of the plasma membrane)|go:"GO:0042126"(nitrate metabolic process)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0043546"(molybdopterin cofactor binding)|go:"GO:0044799"(NarGHI complex)|go:"GO:0046872"(metal ion binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR006468(Nitrate reductase, alpha subunit)|interpro:IPR006655(Molybdopterin oxidoreductase, prokaryotic, conserved site)|interpro:IPR006656(Molybdopterin oxidoreductase)|interpro:IPR006657(Molydopterin dinucleotide-binding region)|interpro:IPR006963(Molybdopterin oxidoreductase Fe4S4 region)|interpro:IPR009010(Aspartate decarboxylase-like fold)|interpro:IPR027467|interpro:IPR028189|interpro:IPR037943|rcsb pdb:1Q16|rcsb pdb:1R27|rcsb pdb:1SIW|rcsb pdb:1Y4Z|rcsb pdb:1Y5I|rcsb pdb:1Y5L|rcsb pdb:1Y5N|rcsb pdb:3EGW|rcsb pdb:3IR5|rcsb pdb:3IR6|rcsb pdb:3IR7|mint:P09152|dip:DIP-10311N	-	crc64:17306C34A57DF1E2	-	figure legend:Fig 4C|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/02/04	2014/10/16	rogid:66OettfLGzuFMcHT2VsxrM/0RV083333	rogid:7OL2t6QltBEY0frD/vkRYKnIm7I83333	intact-crc:688D3280A901EFDF|rigid:25f5LbBsqJPNaQaSWY1R69C855Q	false	sufficient binding region:1..1-186..186	sufficient binding region:1-15	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0AF26	uniprotkb:P09152	intact:EBI-555043|uniprotkb:P11351|ensemblbacteria:AAC74310|ensemblbacteria:BAA36096	intact:EBI-547248|uniprotkb:P78294|ensemblbacteria:AAC74308|ensemblbacteria:BAA36094	psi-mi:narj_ecoli(display_long)|uniprotkb:narJ(gene name)|psi-mi:narJ(display_short)|uniprotkb:b1226(locus name)|uniprotkb:JW1217(locus name)|uniprotkb:Redox enzyme maturation protein NarJ(gene name synonym)	psi-mi:narg_ecoli(display_long)|uniprotkb:narG(gene name)|psi-mi:narG(display_short)|uniprotkb:bisD(gene name synonym)|uniprotkb:narC(gene name synonym)|uniprotkb:b1224(locus name)|uniprotkb:Nitrate reductase A subunit alpha(gene name synonym)|uniprotkb:Quinol-nitrate oxidoreductase subunit alpha(gene name synonym)|uniprotkb:JW1215(locus name)	psi-mi:"MI:0065"(isothermal titration calorimetry)	Zakian et al. (2010)	pubmed:20236317|imex:IM-15683|mint:MINT-7557431	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-3893637|imex:IM-15683-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_415744.1|refseq:WP_000571681.1|dip:DIP-35943N|ensemblbacteria:AAC74310(transcript)|ensemblbacteria:b1226(gene)|ensemblbacteria:BAA36096(gene)|ensemblbacteria:BAA36096(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0016530"(metallochaperone activity)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051131"(chaperone-mediated protein complex assembly)|interpro:IPR003765(Nitrate reductase, delta subunit)|interpro:IPR020945|interpro:IPR036411	refseq:NP_415742.1|refseq:WP_000032939.1|ensemblbacteria:AAC74308(transcript)|ensemblbacteria:b1224(gene)|ensemblbacteria:BAA36094(gene)|ensemblbacteria:BAA36094(transcript)|go:"GO:0008940"(nitrate reductase activity)|go:"GO:0009055"(electron transfer activity)|go:"GO:0009061"(anaerobic respiration)|go:"GO:0016020"(membrane)|go:"GO:0031224"(intrinsic component of membrane)|go:"GO:0031235"(intrinsic component of the cytoplasmic side of the plasma membrane)|go:"GO:0042126"(nitrate metabolic process)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0043546"(molybdopterin cofactor binding)|go:"GO:0044799"(NarGHI complex)|go:"GO:0046872"(metal ion binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR006468(Nitrate reductase, alpha subunit)|interpro:IPR006655(Molybdopterin oxidoreductase, prokaryotic, conserved site)|interpro:IPR006656(Molybdopterin oxidoreductase)|interpro:IPR006657(Molydopterin dinucleotide-binding region)|interpro:IPR006963(Molybdopterin oxidoreductase Fe4S4 region)|interpro:IPR009010(Aspartate decarboxylase-like fold)|interpro:IPR027467|interpro:IPR028189|interpro:IPR037943|rcsb pdb:1Q16|rcsb pdb:1R27|rcsb pdb:1SIW|rcsb pdb:1Y4Z|rcsb pdb:1Y5I|rcsb pdb:1Y5L|rcsb pdb:1Y5N|rcsb pdb:3EGW|rcsb pdb:3IR5|rcsb pdb:3IR6|rcsb pdb:3IR7|mint:P09152|dip:DIP-10311N	-	crc64:17306C34A57DF1E2	-	figure legend:Fig 5, TABLE 2|variable_condition:For first binding site Kd = 3.4 X 10 -9  For second binding site Kd = 3.3 X 10-7|variable_condition_2:Kd =1 X 10-7|comment:Identical results were obtained using NarJ or NarJT and both NarG peptides.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/02/04	2014/10/16	rogid:66OettfLGzuFMcHT2VsxrM/0RV083333	rogid:7OL2t6QltBEY0frD/vkRYKnIm7I83333	intact-crc:35BB28AD4C1E3433|rigid:25f5LbBsqJPNaQaSWY1R69C855Q	false	sufficient binding region:1..1-186..186	sufficient binding region:1-15	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0AF26	uniprotkb:P09152	intact:EBI-555043|uniprotkb:P11351|ensemblbacteria:AAC74310|ensemblbacteria:BAA36096	intact:EBI-547248|uniprotkb:P78294|ensemblbacteria:AAC74308|ensemblbacteria:BAA36094	psi-mi:narj_ecoli(display_long)|uniprotkb:narJ(gene name)|psi-mi:narJ(display_short)|uniprotkb:b1226(locus name)|uniprotkb:JW1217(locus name)|uniprotkb:Redox enzyme maturation protein NarJ(gene name synonym)	psi-mi:narg_ecoli(display_long)|uniprotkb:narG(gene name)|psi-mi:narG(display_short)|uniprotkb:bisD(gene name synonym)|uniprotkb:narC(gene name synonym)|uniprotkb:b1224(locus name)|uniprotkb:Nitrate reductase A subunit alpha(gene name synonym)|uniprotkb:Quinol-nitrate oxidoreductase subunit alpha(gene name synonym)|uniprotkb:JW1215(locus name)	psi-mi:"MI:0065"(isothermal titration calorimetry)	Zakian et al. (2010)	pubmed:20236317|imex:IM-15683|mint:MINT-7557431	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-3892991|imex:IM-15683-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_415744.1|refseq:WP_000571681.1|dip:DIP-35943N|ensemblbacteria:AAC74310(transcript)|ensemblbacteria:b1226(gene)|ensemblbacteria:BAA36096(gene)|ensemblbacteria:BAA36096(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0016530"(metallochaperone activity)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051131"(chaperone-mediated protein complex assembly)|interpro:IPR003765(Nitrate reductase, delta subunit)|interpro:IPR020945|interpro:IPR036411	refseq:NP_415742.1|refseq:WP_000032939.1|ensemblbacteria:AAC74308(transcript)|ensemblbacteria:b1224(gene)|ensemblbacteria:BAA36094(gene)|ensemblbacteria:BAA36094(transcript)|go:"GO:0008940"(nitrate reductase activity)|go:"GO:0009055"(electron transfer activity)|go:"GO:0009061"(anaerobic respiration)|go:"GO:0016020"(membrane)|go:"GO:0031224"(intrinsic component of membrane)|go:"GO:0031235"(intrinsic component of the cytoplasmic side of the plasma membrane)|go:"GO:0042126"(nitrate metabolic process)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0043546"(molybdopterin cofactor binding)|go:"GO:0044799"(NarGHI complex)|go:"GO:0046872"(metal ion binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR006468(Nitrate reductase, alpha subunit)|interpro:IPR006655(Molybdopterin oxidoreductase, prokaryotic, conserved site)|interpro:IPR006656(Molybdopterin oxidoreductase)|interpro:IPR006657(Molydopterin dinucleotide-binding region)|interpro:IPR006963(Molybdopterin oxidoreductase Fe4S4 region)|interpro:IPR009010(Aspartate decarboxylase-like fold)|interpro:IPR027467|interpro:IPR028189|interpro:IPR037943|rcsb pdb:1Q16|rcsb pdb:1R27|rcsb pdb:1SIW|rcsb pdb:1Y4Z|rcsb pdb:1Y5I|rcsb pdb:1Y5L|rcsb pdb:1Y5N|rcsb pdb:3EGW|rcsb pdb:3IR5|rcsb pdb:3IR6|rcsb pdb:3IR7|mint:P09152|dip:DIP-10311N	-	crc64:17306C34A57DF1E2	-	figure legend:Fig 4A/B|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/02/04	2014/10/16	rogid:66OettfLGzuFMcHT2VsxrM/0RV083333	rogid:7OL2t6QltBEY0frD/vkRYKnIm7I83333	intact-crc:DBFBB611DFFBD231|rigid:25f5LbBsqJPNaQaSWY1R69C855Q	false	sufficient binding region:1..1-186..186	sufficient binding region:1-15	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P09152	uniprotkb:P0AF26	intact:EBI-547248|uniprotkb:P78294|ensemblbacteria:AAC74308|ensemblbacteria:BAA36094	intact:EBI-555043|uniprotkb:P11351|ensemblbacteria:AAC74310|ensemblbacteria:BAA36096	psi-mi:narg_ecoli(display_long)|uniprotkb:narG(gene name)|psi-mi:narG(display_short)|uniprotkb:bisD(gene name synonym)|uniprotkb:narC(gene name synonym)|uniprotkb:b1224(locus name)|uniprotkb:Nitrate reductase A subunit alpha(gene name synonym)|uniprotkb:Quinol-nitrate oxidoreductase subunit alpha(gene name synonym)|uniprotkb:JW1215(locus name)	psi-mi:narj_ecoli(display_long)|uniprotkb:narJ(gene name)|psi-mi:narJ(display_short)|uniprotkb:b1226(locus name)|uniprotkb:JW1217(locus name)|uniprotkb:Redox enzyme maturation protein NarJ(gene name synonym)	psi-mi:"MI:0107"(surface plasmon resonance)	Zakian et al. (2010)	pubmed:20236317|imex:IM-15683|mint:MINT-7557431	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-3893659|imex:IM-15683-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_415742.1|refseq:WP_000032939.1|ensemblbacteria:AAC74308(transcript)|ensemblbacteria:b1224(gene)|ensemblbacteria:BAA36094(gene)|ensemblbacteria:BAA36094(transcript)|go:"GO:0008940"(nitrate reductase activity)|go:"GO:0009055"(electron transfer activity)|go:"GO:0009061"(anaerobic respiration)|go:"GO:0016020"(membrane)|go:"GO:0031224"(intrinsic component of membrane)|go:"GO:0031235"(intrinsic component of the cytoplasmic side of the plasma membrane)|go:"GO:0042126"(nitrate metabolic process)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0043546"(molybdopterin cofactor binding)|go:"GO:0044799"(NarGHI complex)|go:"GO:0046872"(metal ion binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR006468(Nitrate reductase, alpha subunit)|interpro:IPR006655(Molybdopterin oxidoreductase, prokaryotic, conserved site)|interpro:IPR006656(Molybdopterin oxidoreductase)|interpro:IPR006657(Molydopterin dinucleotide-binding region)|interpro:IPR006963(Molybdopterin oxidoreductase Fe4S4 region)|interpro:IPR009010(Aspartate decarboxylase-like fold)|interpro:IPR027467|interpro:IPR028189|interpro:IPR037943|rcsb pdb:1Q16|rcsb pdb:1R27|rcsb pdb:1SIW|rcsb pdb:1Y4Z|rcsb pdb:1Y5I|rcsb pdb:1Y5L|rcsb pdb:1Y5N|rcsb pdb:3EGW|rcsb pdb:3IR5|rcsb pdb:3IR6|rcsb pdb:3IR7|mint:P09152|dip:DIP-10311N	refseq:NP_415744.1|refseq:WP_000571681.1|dip:DIP-35943N|ensemblbacteria:AAC74310(transcript)|ensemblbacteria:b1226(gene)|ensemblbacteria:BAA36096(gene)|ensemblbacteria:BAA36096(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0016530"(metallochaperone activity)|go:"GO:0042128"(nitrate assimilation)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051131"(chaperone-mediated protein complex assembly)|interpro:IPR003765(Nitrate reductase, delta subunit)|interpro:IPR020945|interpro:IPR036411	-	-	crc64:17306C34A57DF1E2	figure legend:none - see  text under Fig 6|kinetics:"pH 7:-The results indicated the existence of a minor population (27%) with a high affinity (Kd= 4.4 +/- 3 × 10−9 m) and a major population (73%) with a lower affinity (Kd = 81 +/- 36 × 10−9 m). Analysis of the BIAcore experiments performed at pH 8 could only be fitted with the 1 : 1 Langmuir model of simple binding, confirming the existence of a single state of NarJ at this pH."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:4.4x10^-9(molar)|kd:81.0x10^-9(molar)	2010/02/04	2014/10/16	rogid:7OL2t6QltBEY0frD/vkRYKnIm7I83333	rogid:66OettfLGzuFMcHT2VsxrM/0RV083333	intact-crc:1318CFDB3E8A62B8|rigid:25f5LbBsqJPNaQaSWY1R69C855Q	false	sufficient binding region:1-15	his tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)