#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q22A77	uniprotkb:Q8MXZ9	intact:EBI-2660533|ensemblprotists:EAR82180	intact:EBI-2660548	psi-mi:q22a77_tetts(display_long)|uniprotkb:TTHERM_01276320(orf name)	psi-mi:q8mxz9_tetth(display_long)|uniprotkb:TWI1(gene name)|psi-mi:TWI1(display_short)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Noto et al. (2010)	imex:IM-13649|pubmed:20211138	taxid:312017(tetts)|taxid:312017("Tetrahymena thermophila (strain SB210)")	taxid:5911(tetth)|taxid:5911(Tetrahymena thermophila)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2660530|imex:IM-13649-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_001029843.2|ensemblprotists:EAR82180(transcript)|ensemblprotists:TTHERM_01276320(gene)	interpro:IPR003100(Argonaute and Dicer protein, PAZ)|interpro:IPR003165(Stem cell self-renewal protein Piwi)|interpro:IPR012337(Polynucleotidyl transferase, Ribonuclease H fold)|go:"GO:0003676"(nucleic acid binding)|interpro:IPR036085|interpro:IPR036397	psi-mi:"MI:0469"(intact)	-	-	figure legend:Fig 3B.|comment:A substantially higher amount of Twi1p was precipitated from the GIW1-HA strains than from the nontagged strains at all developmental stages tested, confirming that Twi1p and Giw1p are found in the same complex.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:274(theth)|taxid:274(Thermus thermophilus)	-	2010/05/20	2014/10/16	rogid:GM/psiZ1GBJQpozLowh0Lrj9YNc312017	rogid:cap9AQiU25Wkt/t39XByHE+THWE5911	intact-crc:33E0AB094AE34E50|rigid:TApWSh153eHAdb3HPoB3+WDYQxo	false	binding-associated region:?-?|ha tag:c-c	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q22A77	uniprotkb:Q8MXZ9	intact:EBI-2660533|ensemblprotists:EAR82180	intact:EBI-2660548	psi-mi:q22a77_tetts(display_long)|uniprotkb:TTHERM_01276320(orf name)	psi-mi:q8mxz9_tetth(display_long)|uniprotkb:TWI1(gene name)|psi-mi:TWI1(display_short)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Noto et al. (2010)	imex:IM-13649|pubmed:20211138	taxid:312017(tetts)|taxid:312017("Tetrahymena thermophila (strain SB210)")	taxid:5911(tetth)|taxid:5911(Tetrahymena thermophila)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2660567|imex:IM-13649-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_001029843.2|ensemblprotists:EAR82180(transcript)|ensemblprotists:TTHERM_01276320(gene)	interpro:IPR003100(Argonaute and Dicer protein, PAZ)|interpro:IPR003165(Stem cell self-renewal protein Piwi)|interpro:IPR012337(Polynucleotidyl transferase, Ribonuclease H fold)|go:"GO:0003676"(nucleic acid binding)|interpro:IPR036085|interpro:IPR036397	psi-mi:"MI:0469"(intact)	-	-	figure legend:Fig 3C, 6C, S5.|comment:"Giw1p can interact with Twi1p in the absence of scnRNA in cell lysate. No detectable Giw1p was precipitated with (Slicer-dead mutants) Twi1p-D526N  nor Twi1p-H745Q."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:274(theth)|taxid:274(Thermus thermophilus)	-	2010/05/20	2014/10/16	rogid:GM/psiZ1GBJQpozLowh0Lrj9YNc312017	rogid:cap9AQiU25Wkt/t39XByHE+THWE5911	intact-crc:8F5981274350B7E5|rigid:TApWSh153eHAdb3HPoB3+WDYQxo	false	-	mutation disrupting interaction:526-526|mutation disrupting interaction:745-745|ha tag:?-?|flag tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q8MXZ9	uniprotkb:B8XQC5	intact:EBI-2660548	intact:EBI-2660711	psi-mi:q8mxz9_tetth(display_long)|uniprotkb:TWI1(gene name)|psi-mi:TWI1(display_short)	psi-mi:b8xqc5_tetth(display_long)|uniprotkb:WAG1(gene name)|psi-mi:WAG1(display_short)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Noto et al. (2010)	imex:IM-13649|pubmed:20211138	taxid:5911(tetth)|taxid:5911(Tetrahymena thermophila)	taxid:5911(tetth)|taxid:5911(Tetrahymena thermophila)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2660677|imex:IM-13649-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR003100(Argonaute and Dicer protein, PAZ)|interpro:IPR003165(Stem cell self-renewal protein Piwi)|interpro:IPR012337(Polynucleotidyl transferase, Ribonuclease H fold)|go:"GO:0003676"(nucleic acid binding)|interpro:IPR036085|interpro:IPR036397	-	psi-mi:"MI:0469"(intact)	-	-	figure legend:Fig S3A.|comment:Small amounts of Ema1p and Wag1p were coimmunoprecipitated with Twi1p.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:274(theth)|taxid:274(Thermus thermophilus)	-	2010/05/20	2014/10/16	rogid:cap9AQiU25Wkt/t39XByHE+THWE5911	rogid:StVeCH23iemurlNCX/0QKWYBnQs5911	intact-crc:15734186070C7EC3|rigid:ZnVnYAUiCoJOZqMYQbeDgQ/RecY	false	ha tag:?-?|flag tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q8MXZ9	uniprotkb:A4F2M3	intact:EBI-2660548	intact:EBI-2660695	psi-mi:q8mxz9_tetth(display_long)|uniprotkb:TWI1(gene name)|psi-mi:TWI1(display_short)	psi-mi:a4f2m3_tetth(display_long)|uniprotkb:EMA1(gene name)|psi-mi:EMA1(display_short)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Noto et al. (2010)	imex:IM-13649|pubmed:20211138	taxid:5911(tetth)|taxid:5911(Tetrahymena thermophila)	taxid:5911(tetth)|taxid:5911(Tetrahymena thermophila)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2660677|imex:IM-13649-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR003100(Argonaute and Dicer protein, PAZ)|interpro:IPR003165(Stem cell self-renewal protein Piwi)|interpro:IPR012337(Polynucleotidyl transferase, Ribonuclease H fold)|go:"GO:0003676"(nucleic acid binding)|interpro:IPR036085|interpro:IPR036397	interpro:IPR014001(DEAD-like helicase, N-terminal)|interpro:IPR011545(DNA/RNA helicase, DEAD/DEAH box type, N-terminal)|interpro:IPR007502(Helicase-associated region)|interpro:IPR001650(DNA/RNA helicase, C-terminal)|interpro:IPR027417|go:"GO:0003676"(nucleic acid binding)|go:"GO:0004386"(helicase activity)|go:"GO:0005524"(ATP binding)	psi-mi:"MI:0469"(intact)	-	-	figure legend:Fig S3A.|comment:Small amounts of Ema1p and Wag1p were coimmunoprecipitated with Twi1p.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:274(theth)|taxid:274(Thermus thermophilus)	-	2010/05/20	2014/10/16	rogid:cap9AQiU25Wkt/t39XByHE+THWE5911	rogid:D+Lx7NqLCUQcWfZAr41lTuVNBhg5911	intact-crc:15734186070C7EC3|rigid:ZnVnYAUiCoJOZqMYQbeDgQ/RecY	false	ha tag:?-?|flag tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q8MXZ9	uniprotkb:Q22A77	intact:EBI-2660548	intact:EBI-2660533|ensemblprotists:EAR82180	psi-mi:q8mxz9_tetth(display_long)|uniprotkb:TWI1(gene name)|psi-mi:TWI1(display_short)	psi-mi:q22a77_tetts(display_long)|uniprotkb:TTHERM_01276320(orf name)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Noto et al. (2010)	imex:IM-13649|pubmed:20211138	taxid:5911(tetth)|taxid:5911(Tetrahymena thermophila)	taxid:312017(tetts)|taxid:312017("Tetrahymena thermophila (strain SB210)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2660677|imex:IM-13649-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR003100(Argonaute and Dicer protein, PAZ)|interpro:IPR003165(Stem cell self-renewal protein Piwi)|interpro:IPR012337(Polynucleotidyl transferase, Ribonuclease H fold)|go:"GO:0003676"(nucleic acid binding)|interpro:IPR036085|interpro:IPR036397	refseq:XP_001029843.2|ensemblprotists:EAR82180(transcript)|ensemblprotists:TTHERM_01276320(gene)	psi-mi:"MI:0469"(intact)	-	-	figure legend:Fig S3A.|comment:Small amounts of Ema1p and Wag1p were coimmunoprecipitated with Twi1p.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:274(theth)|taxid:274(Thermus thermophilus)	-	2010/05/20	2014/10/16	rogid:cap9AQiU25Wkt/t39XByHE+THWE5911	rogid:GM/psiZ1GBJQpozLowh0Lrj9YNc312017	intact-crc:15734186070C7EC3|rigid:ZnVnYAUiCoJOZqMYQbeDgQ/RecY	false	ha tag:?-?|flag tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q8MXZ9	uniprotkb:Q22A77	intact:EBI-2660548	intact:EBI-2660533|ensemblprotists:EAR82180	psi-mi:q8mxz9_tetth(display_long)|uniprotkb:TWI1(gene name)|psi-mi:TWI1(display_short)	psi-mi:q22a77_tetts(display_long)|uniprotkb:TTHERM_01276320(orf name)	psi-mi:"MI:0096"(pull down)	Noto et al. (2010)	imex:IM-13649|pubmed:20211138	taxid:5911(tetth)|taxid:5911(Tetrahymena thermophila)	taxid:312017(tetts)|taxid:312017("Tetrahymena thermophila (strain SB210)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2660596|imex:IM-13649-6	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR003100(Argonaute and Dicer protein, PAZ)|interpro:IPR003165(Stem cell self-renewal protein Piwi)|interpro:IPR012337(Polynucleotidyl transferase, Ribonuclease H fold)|go:"GO:0003676"(nucleic acid binding)|interpro:IPR036085|interpro:IPR036397	refseq:XP_001029843.2|ensemblprotists:EAR82180(transcript)|ensemblprotists:TTHERM_01276320(gene)	psi-mi:"MI:0469"(intact)	-	-	figure legend:Fig 3D, 6D.|comment:Giw1p coprecipitated with the PAZ and the Piwi domains, but not with other domains of Twi1p. The amount of Giw1p coprecipitated with GST-Twi1p was greatly reduced when GST-Twi1p was preincubated with a guide and a noncleavable target RNA, indicating that the presence of double-stranded scnRNAs in Twi1p inhibits the Giw1p-Twi1p interaction.|antagonist:Double stranded scnRNAs.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/05/20	2014/10/16	rogid:cap9AQiU25Wkt/t39XByHE+THWE5911	rogid:GM/psiZ1GBJQpozLowh0Lrj9YNc312017	intact-crc:074921DDB70FE30C|rigid:TApWSh153eHAdb3HPoB3+WDYQxo	false	glutathione s tranferase tag:?-?|sufficient binding region:166-306(IPR003100)|sufficient binding region:445-756(IPR003165)	35s radiolabel:?-?	-	-	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)
uniprotkb:Q22A77	uniprotkb:Q8MXZ9	intact:EBI-2660533|ensemblprotists:EAR82180	intact:EBI-2660548	psi-mi:q22a77_tetts(display_long)|uniprotkb:TTHERM_01276320(orf name)	psi-mi:q8mxz9_tetth(display_long)|uniprotkb:TWI1(gene name)|psi-mi:TWI1(display_short)	psi-mi:"MI:0096"(pull down)	Noto et al. (2010)	imex:IM-13649|pubmed:20211138	taxid:312017(tetts)|taxid:312017("Tetrahymena thermophila (strain SB210)")	taxid:5911(tetth)|taxid:5911(Tetrahymena thermophila)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2677026|imex:IM-13649-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_001029843.2|ensemblprotists:EAR82180(transcript)|ensemblprotists:TTHERM_01276320(gene)	interpro:IPR003100(Argonaute and Dicer protein, PAZ)|interpro:IPR003165(Stem cell self-renewal protein Piwi)|interpro:IPR012337(Polynucleotidyl transferase, Ribonuclease H fold)|go:"GO:0003676"(nucleic acid binding)|interpro:IPR036085|interpro:IPR036397	psi-mi:"MI:0469"(intact)	-	-	figure legend:Fig 3E.|comment:"Three of the six segments of Giw1p (N1, N3, and C2) were efficiently coprecipitated with PAZ and Piwi domains of Twi1p.  Small amounts of the other three segments (N2, C1, and C3) were also coprecipitated with PAZ and Piwi domains."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/05/20	2014/10/16	rogid:GM/psiZ1GBJQpozLowh0Lrj9YNc312017	rogid:cap9AQiU25Wkt/t39XByHE+THWE5911	intact-crc:7685606F51F0DC86|rigid:TApWSh153eHAdb3HPoB3+WDYQxo	false	35s radiolabel:?-?	sufficient binding region:166-306(IPR003100)|glutathione s tranferase tag:?-?|sufficient binding region:445-756(IPR003165)	-	-	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)
uniprotkb:Q8MXZ9	uniprotkb:Q22A77	intact:EBI-2660548	intact:EBI-2660533|ensemblprotists:EAR82180	psi-mi:q8mxz9_tetth(display_long)|uniprotkb:TWI1(gene name)|psi-mi:TWI1(display_short)	psi-mi:q22a77_tetts(display_long)|uniprotkb:TTHERM_01276320(orf name)	psi-mi:"MI:0096"(pull down)	Noto et al. (2010)	imex:IM-13649|pubmed:20211138	taxid:5911(tetth)|taxid:5911(Tetrahymena thermophila)	taxid:312017(tetts)|taxid:312017("Tetrahymena thermophila (strain SB210)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2660907|imex:IM-13649-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR003100(Argonaute and Dicer protein, PAZ)|interpro:IPR003165(Stem cell self-renewal protein Piwi)|interpro:IPR012337(Polynucleotidyl transferase, Ribonuclease H fold)|go:"GO:0003676"(nucleic acid binding)|interpro:IPR036085|interpro:IPR036397	refseq:XP_001029843.2|ensemblprotists:EAR82180(transcript)|ensemblprotists:TTHERM_01276320(gene)	psi-mi:"MI:0469"(intact)	-	-	figure legend:Fig S3B.|comment:"The 115 kDa protein was identified by mass spectrometry  and named  Giw1p (gentleman-in-waiting)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:5911(tetth)|taxid:5911(Tetrahymena thermophila)	-	2010/05/20	2014/10/16	rogid:cap9AQiU25Wkt/t39XByHE+THWE5911	rogid:GM/psiZ1GBJQpozLowh0Lrj9YNc312017	intact-crc:8226B6DFF738F271|rigid:TApWSh153eHAdb3HPoB3+WDYQxo	false	ha tag:?-?|flag tag:?-?	-	-	-	psi-mi:"MI:0102"(sequence tag identification)	psi-mi:"MI:0102"(sequence tag identification)
uniprotkb:Q8MXZ9	uniprotkb:Q22A77	intact:EBI-2660548	intact:EBI-2660533|ensemblprotists:EAR82180	psi-mi:q8mxz9_tetth(display_long)|uniprotkb:TWI1(gene name)|psi-mi:TWI1(display_short)	psi-mi:q22a77_tetts(display_long)|uniprotkb:TTHERM_01276320(orf name)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Noto et al. (2010)	imex:IM-13649|pubmed:20211138	taxid:5911(tetth)|taxid:5911(Tetrahymena thermophila)	taxid:312017(tetts)|taxid:312017("Tetrahymena thermophila (strain SB210)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2661083|imex:IM-13649-8	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR003100(Argonaute and Dicer protein, PAZ)|interpro:IPR003165(Stem cell self-renewal protein Piwi)|interpro:IPR012337(Polynucleotidyl transferase, Ribonuclease H fold)|go:"GO:0003676"(nucleic acid binding)|interpro:IPR036085|interpro:IPR036397	refseq:XP_001029843.2|ensemblprotists:EAR82180(transcript)|ensemblprotists:TTHERM_01276320(gene)	psi-mi:"MI:0469"(intact)	-	-	figure legend:Fig S3D.|comment:Giw1p was coimmunoprecipitated with Twi1p from EMA1 KO strains, in which the Twi1p-ncRNA interaction is impaired.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:274(theth)|taxid:274(Thermus thermophilus)	-	2010/05/20	2014/10/16	rogid:cap9AQiU25Wkt/t39XByHE+THWE5911	rogid:GM/psiZ1GBJQpozLowh0Lrj9YNc312017	intact-crc:017D394CDD3418A0|rigid:TApWSh153eHAdb3HPoB3+WDYQxo	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q8MXZ9	uniprotkb:Q22A77	intact:EBI-2660548	intact:EBI-2660533|ensemblprotists:EAR82180	psi-mi:q8mxz9_tetth(display_long)|uniprotkb:TWI1(gene name)|psi-mi:TWI1(display_short)	psi-mi:q22a77_tetts(display_long)|uniprotkb:TTHERM_01276320(orf name)	psi-mi:"MI:0096"(pull down)	Noto et al. (2010)	imex:IM-13649|pubmed:20211138	taxid:5911(tetth)|taxid:5911(Tetrahymena thermophila)	taxid:312017(tetts)|taxid:312017("Tetrahymena thermophila (strain SB210)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2661466|imex:IM-13649-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR003100(Argonaute and Dicer protein, PAZ)|interpro:IPR003165(Stem cell self-renewal protein Piwi)|interpro:IPR012337(Polynucleotidyl transferase, Ribonuclease H fold)|go:"GO:0003676"(nucleic acid binding)|interpro:IPR036085|interpro:IPR036397	refseq:XP_001029843.2|ensemblprotists:EAR82180(transcript)|ensemblprotists:TTHERM_01276320(gene)	psi-mi:"MI:0469"(intact)	-	-	figure legend:Fig S3E.|comment:"Three of the six segments of Giw1p (N1, N3, and C2) were efficiently coprecipitated with PAZ and Piwi domains of Twi1p.  Small amounts of the other three segments (N2, C1, and C3) were also coprecipitated with PAZ and Piwi domains."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/05/20	2014/10/16	rogid:cap9AQiU25Wkt/t39XByHE+THWE5911	rogid:GM/psiZ1GBJQpozLowh0Lrj9YNc312017	intact-crc:74FEDE409604AD5F|rigid:TApWSh153eHAdb3HPoB3+WDYQxo	false	sufficient binding region:166-306(IPR003100)|his tag:?-?|sufficient binding region:445-756(IPR003165)	sufficient binding region:361-524|glutathione s tranferase tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)