#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P31346	uniprotkb:P10775	intact:EBI-2944901	intact:EBI-2944741|ensembl:ENSSSCP00055002749|ensembl:ENSSSCP00055002762|ensembl:ENSSSCP00055002773|ensembl:ENSSSCP00055002805|ensembl:ENSSSCP00070046958|ensembl:ENSSSCP00070046962	psi-mi:angi_pig(display_long)|uniprotkb:ANG(gene name)|psi-mi:ANG(display_short)|uniprotkb:Ribonuclease 5(gene name synonym)	psi-mi:rini_pig(display_long)|uniprotkb:RNH1(gene name)|psi-mi:RNH1(display_short)|uniprotkb:RI(gene name synonym)|uniprotkb:RNH(gene name synonym)|uniprotkb:Ribonuclease/angiogenin inhibitor 1(gene name synonym)	psi-mi:"MI:0405"(competition binding)	Dickson et al. (2009)	pubmed:19354288|imex:IM-20527	taxid:9823(pig)|taxid:9823("Sus scrofa (Pig)")	taxid:9823(pig)|taxid:9823("Sus scrofa (Pig)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2944740|imex:IM-20527-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0001525"(angiogenesis)|go:"GO:0003677"(DNA binding)|go:"GO:0004519"(endonuclease activity)|go:"GO:0004540"(ribonuclease activity)|go:"GO:0005634"(nucleus)|go:"GO:0005730"(nucleolus)|go:"GO:0017148"(negative regulation of translation)|go:"GO:0019731"(antibacterial humoral response)|go:"GO:0030154"(cell differentiation)|go:"GO:0005615"(extracellular space)|go:"GO:0030426"(growth cone)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043025"(neuronal cell body)|go:"GO:0045087"(innate immune response)|go:"GO:0050830"(defense response to Gram-positive bacterium)|go:"GO:0061844"(antimicrobial humoral immune response mediated by antimicrobial peptide)|interpro:IPR001427(Pancreatic ribonuclease)|interpro:IPR023411|interpro:IPR023412|interpro:IPR036816	ensembl:ENSSSCG00055001944(gene)|ensembl:ENSSSCG00070027590(gene)|ensembl:ENSSSCT00055003614(transcript)|ensembl:ENSSSCT00055003629(transcript)|ensembl:ENSSSCT00055003640(transcript)|ensembl:ENSSSCT00055003675(transcript)|ensembl:ENSSSCT00070055317(transcript)|ensembl:ENSSSCT00070055321(transcript)|go:"GO:0005737"(cytoplasm)|interpro:IPR001611(Leucine-rich repeat)|interpro:IPR006553(Leucine-rich repeat, cysteine-containing subtype)|interpro:IPR032675|interpro:IPR041302|rcsb pdb:1DFJ|rcsb pdb:2BNH|refseq:XP_013847653.1	-	-	-	comment:"The G85R/G86R substitutions lead to a marked decrease in affinity for RI (Kd = 5nm). Kd for RI complex with G88R RNase1 is 0.6nM."|figure legend:Table 1.|kinetic_conditions:"30 min at 23 degrees C. Fluorescence excitation wavelength:t 491 nm; emission at 511 nm."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/11/15	2014/10/16	rogid:5foQaa4lTHCS3WMRBz6FRerdw889823	rogid:Be8MTAhh3znxCOLI4tx1tWkkYJg9823	intact-crc:8B3B5F688936CF0B|rigid:T6FmAlAHekCXh4KWVbOmzg+OKSw	false	mutation decreasing interaction:84-84,85-85	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P31346	uniprotkb:P00671	intact:EBI-2944901	intact:EBI-2944924	psi-mi:angi_pig(display_long)|uniprotkb:ANG(gene name)|psi-mi:ANG(display_short)|uniprotkb:Ribonuclease 5(gene name synonym)	psi-mi:rnas1_pig(display_long)|uniprotkb:RNASE1(gene name)|psi-mi:RNASE1(display_short)|uniprotkb:RNS1(gene name synonym)|uniprotkb:RNase 1(gene name synonym)|uniprotkb:RNase A(gene name synonym)	psi-mi:"MI:0405"(competition binding)	Dickson et al. (2009)	pubmed:19354288|imex:IM-20527	taxid:9823(pig)|taxid:9823("Sus scrofa (Pig)")	taxid:9823(pig)|taxid:9823("Sus scrofa (Pig)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2944740|imex:IM-20527-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0001525"(angiogenesis)|go:"GO:0003677"(DNA binding)|go:"GO:0004519"(endonuclease activity)|go:"GO:0004540"(ribonuclease activity)|go:"GO:0005634"(nucleus)|go:"GO:0005730"(nucleolus)|go:"GO:0017148"(negative regulation of translation)|go:"GO:0019731"(antibacterial humoral response)|go:"GO:0030154"(cell differentiation)|go:"GO:0005615"(extracellular space)|go:"GO:0030426"(growth cone)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043025"(neuronal cell body)|go:"GO:0045087"(innate immune response)|go:"GO:0050830"(defense response to Gram-positive bacterium)|go:"GO:0061844"(antimicrobial humoral immune response mediated by antimicrobial peptide)|interpro:IPR001427(Pancreatic ribonuclease)|interpro:IPR023411|interpro:IPR023412|interpro:IPR036816	go:"GO:0003676"(nucleic acid binding)|go:"GO:0004522"(ribonuclease A activity)|go:"GO:0004540"(ribonuclease activity)|go:"GO:0005576"(extracellular region)|go:"GO:0016829"(lyase activity)|go:"GO:0090501"(RNA phosphodiester bond hydrolysis)|interpro:IPR001427(Pancreatic ribonuclease)|interpro:IPR023411|interpro:IPR023412|interpro:IPR036816	-	-	-	comment:"The G85R/G86R substitutions lead to a marked decrease in affinity for RI (Kd = 5nm). Kd for RI complex with G88R RNase1 is 0.6nM."|figure legend:Table 1.|kinetic_conditions:"30 min at 23 degrees C. Fluorescence excitation wavelength:t 491 nm; emission at 511 nm."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/11/15	2014/10/16	rogid:5foQaa4lTHCS3WMRBz6FRerdw889823	rogid:nbKVHb0xJW/uHqkuzn4g/41mU2c9823	intact-crc:8B3B5F688936CF0B|rigid:T6FmAlAHekCXh4KWVbOmzg+OKSw	false	mutation decreasing interaction:84-84,85-85	fluorescein label:?-?|mutation:88-88	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
intact:EBI-2944965	uniprotkb:P00671	-	intact:EBI-2944924	psi-mi:fam_nucleotide_tamra(display_short)|psi-mi:EBI-2944965(display_long)	psi-mi:rnas1_pig(display_long)|uniprotkb:RNASE1(gene name)|psi-mi:RNASE1(display_short)|uniprotkb:RNS1(gene name synonym)|uniprotkb:RNase 1(gene name synonym)|uniprotkb:RNase A(gene name synonym)	psi-mi:"MI:0920"(ribonuclease assay)	Dickson et al. (2009)	pubmed:19354288|imex:IM-20527	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9823(pig)|taxid:9823("Sus scrofa (Pig)")	psi-mi:"MI:0902"(rna cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2944948|imex:IM-20527-2	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0320"(ribonucleic acid)	psi-mi:"MI:0326"(protein)	-	go:"GO:0003676"(nucleic acid binding)|go:"GO:0004522"(ribonuclease A activity)|go:"GO:0004540"(ribonuclease activity)|go:"GO:0005576"(extracellular region)|go:"GO:0016829"(lyase activity)|go:"GO:0090501"(RNA phosphodiester bond hydrolysis)|interpro:IPR001427(Pancreatic ribonuclease)|interpro:IPR023411|interpro:IPR023412|interpro:IPR036816	-	comment:"a tetranucleotide with a 5',6-carboxyfluorescein label (6-FAM) and a 3',6-carboxy-tetramethylrhodamine (6-TAMRA) label:"	-	comment:"Km for Wild-type RNase A is(2.1 ± 0.4) × 107 (M-1s-1) wheras for the mutant G88R RNase A it is(0.5 ± 0.3) × 107 (M-1s-1)."|figure legend:Table 1.|kinetic_conditions:catalysis of 6-FAM-dArUdAdA-6-TAMRA cleavage at pH 6.0 and 23 °C.|caution:All interactors have been assumed to be porcine in origin.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/11/15	2014/10/16	-	rogid:nbKVHb0xJW/uHqkuzn4g/41mU2c9823	intact-crc:A7799B578DF724CD	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
intact:EBI-2944965	uniprotkb:P31346	-	intact:EBI-2944901	psi-mi:fam_nucleotide_tamra(display_short)|psi-mi:EBI-2944965(display_long)	psi-mi:angi_pig(display_long)|uniprotkb:ANG(gene name)|psi-mi:ANG(display_short)|uniprotkb:Ribonuclease 5(gene name synonym)	psi-mi:"MI:0920"(ribonuclease assay)	Dickson et al. (2009)	pubmed:19354288|imex:IM-20527	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9823(pig)|taxid:9823("Sus scrofa (Pig)")	psi-mi:"MI:0902"(rna cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2944968|imex:IM-20527-3	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0320"(ribonucleic acid)	psi-mi:"MI:0326"(protein)	-	go:"GO:0001525"(angiogenesis)|go:"GO:0003677"(DNA binding)|go:"GO:0004519"(endonuclease activity)|go:"GO:0004540"(ribonuclease activity)|go:"GO:0005634"(nucleus)|go:"GO:0005730"(nucleolus)|go:"GO:0017148"(negative regulation of translation)|go:"GO:0019731"(antibacterial humoral response)|go:"GO:0030154"(cell differentiation)|go:"GO:0005615"(extracellular space)|go:"GO:0030426"(growth cone)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043025"(neuronal cell body)|go:"GO:0045087"(innate immune response)|go:"GO:0050830"(defense response to Gram-positive bacterium)|go:"GO:0061844"(antimicrobial humoral immune response mediated by antimicrobial peptide)|interpro:IPR001427(Pancreatic ribonuclease)|interpro:IPR023411|interpro:IPR023412|interpro:IPR036816	-	comment:"a tetranucleotide with a 5',6-carboxyfluorescein label (6-FAM) and a 3',6-carboxy-tetramethylrhodamine (6-TAMRA) label:"	-	comment:"The catalytic activity of the mutant is not affected - Km for both is ~75 (M-1s-1)."|figure legend:Table 1.|kinetic_conditions:catalysis of 6-FAM-dArUdAdA-6-TAMRA cleavage at pH 6.0 and 23 °C.|caution:All interactors have been assumed to be porcine in origin.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2010/11/15	2014/10/16	-	rogid:5foQaa4lTHCS3WMRBz6FRerdw889823	intact-crc:E23DEDF20AD28539	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)