#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P06784	uniprotkb:P14681	intact:EBI-18389|uniprotkb:D6VRJ2|ensemblfungi:YDL159W	intact:EBI-9945|uniprotkb:Q45U43|uniprotkb:D6VUH8|ensemblfungi:YGR040W	psi-mi:ste7_yeast(display_long)|uniprotkb:STE7(gene name)|psi-mi:STE7(display_short)|uniprotkb:D1525(orf name)|uniprotkb:YDL159W(locus name)	psi-mi:kss1_yeast(display_long)|uniprotkb:KSS1(gene name)|psi-mi:KSS1(display_short)|uniprotkb:Kinase suppressor of SST2(gene name synonym)|uniprotkb:YGR040W(locus name)	psi-mi:"MI:0424"(protein kinase assay)	Good et al. (2009)	imex:IM-12170|pubmed:19303851	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0217"(phosphorylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2325797|imex:IM-12170-3	-	-	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_010122.1|sgd:S000002318|reactome:R-SCE-112411|reactome:R-SCE-445144|reactome:R-SCE-5674135|reactome:R-SCE-5674499|ensemblfungi:YDL159W(gene)|ensemblfungi:YDL159W_mRNA(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0000196"(cell wall integrity MAPK cascade)|go:"GO:0001402"(signal transduction involved in filamentous growth)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007124"(pseudohyphal growth)|go:"GO:0010525"(regulation of transposition, RNA-mediated)|go:"GO:0043332"(mating projection tip)|go:"GO:0071507"(pheromone response MAPK cascade)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|reactome:R-SCE-110056|dip:DIP-9N|mint:P06784	sgd:S000003272|ensemblfungi:YGR040W(gene)|ensemblfungi:YGR040W_mRNA(transcript)|go:"GO:0000750"(pheromone-dependent signal transduction involved in conjugation with cellular fusion)|go:"GO:0001402"(signal transduction involved in filamentous growth)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004707"(MAP kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005935"(cellular bud neck)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007049"(cell cycle)|go:"GO:0008134"(transcription factor binding)|go:"GO:0010973"(positive regulation of division septum assembly)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042597"(periplasmic space)|go:"GO:0043433"(negative regulation of DNA-binding transcription factor activity)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR003527(MAP kinase, conserved site)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|reactome:R-SCE-111995|reactome:R-SCE-112411|reactome:R-SCE-198753|reactome:R-SCE-202670|reactome:R-SCE-2559582|reactome:R-SCE-375165|reactome:R-SCE-437239|reactome:R-SCE-5687128|reactome:R-SCE-6798695|reactome:R-SCE-881907|reactome:R-SCE-9634635|refseq:NP_011554.3|reactome:R-SCE-112409|reactome:R-SCE-3371453|reactome:R-SCE-445144|reactome:R-SCE-5673001|reactome:R-SCE-5674135|reactome:R-SCE-5674499|dip:DIP-60N|reactome:R-SCE-170968|reactome:R-SCE-450341	go:"GO:0006468"(protein phosphorylation)|go:"GO:0004672"(protein kinase activity)	crc64:C8A75899CFBE8BDE	crc64:5FA42161B5FD51B5	resulting-ptm:Phosphorylation, and activation, of KSS1|figure legend:Figs. 2B, 4B, Supp. Fig. 2B|comment:Michaelis-Menten plots showed Ste7 phosphorylation of Kss1 does not require the presence of Ste5, and had no effect of the km or Kcat of the reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2009/05/11	2014/10/16	rogid:dyC78MRkFAByOXZMkYmZ8nul+dU559292	rogid:xE1y1jku+iWy4xzv9wUCul1AFZs559292	intact-crc:F1BD448488AFD5C0|rigid:v9VALaELwEARboEE7iHaBr1KUi0	false	glutathione s tranferase tag:n-n|mutation:359-359,363-363|his tag:c-c	his tag:n-n	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P16892	uniprotkb:P32917	intact:EBI-7193|uniprotkb:D6VPY4|ensemblfungi:YBL016W	intact:EBI-18373|uniprotkb:D6VS89|ensemblfungi:YDR103W	psi-mi:fus3_yeast(display_long)|uniprotkb:FUS3(gene name)|psi-mi:FUS3(display_short)|uniprotkb:DAC2(gene name synonym)|uniprotkb:YBL016W(locus name)|uniprotkb:YBL0303(orf name)|uniprotkb:YBL03.21(orf name)	psi-mi:ste5_yeast(display_long)|uniprotkb:STE5(gene name)|psi-mi:STE5(display_short)|uniprotkb:NUL3(gene name synonym)|uniprotkb:YDR103W(locus name)|uniprotkb:YD8557.12(orf name)	psi-mi:"MI:0424"(protein kinase assay)	Good et al. (2009)	imex:IM-12170|pubmed:19303851	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0217"(phosphorylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2326314|imex:IM-12170-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0684"(ancillary)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009537.1|sgd:S000000112|dip:DIP-714N|mint:P16892|ensemblfungi:YBL016W(gene)|ensemblfungi:YBL016W_mRNA(transcript)|go:"GO:0000750"(pheromone-dependent signal transduction involved in conjugation with cellular fusion)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004707"(MAP kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007049"(cell cycle)|go:"GO:0010494"(cytoplasmic stress granule)|go:"GO:0010526"(negative regulation of transposition, RNA-mediated)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042597"(periplasmic space)|go:"GO:0042802"(identical protein binding)|go:"GO:0043332"(mating projection tip)|go:"GO:0043409"(negative regulation of MAPK cascade)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0046827"(positive regulation of protein export from nucleus)|go:"GO:0051301"(cell division)|go:"GO:0071507"(pheromone response MAPK cascade)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR003527(MAP kinase, conserved site)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|rcsb pdb:2B9F|rcsb pdb:2B9H|rcsb pdb:2B9I|rcsb pdb:2B9J|rcsb pdb:2F49|rcsb pdb:2F9G|rcsb pdb:2FA2|reactome:R-SCE-110056|reactome:R-SCE-111995|reactome:R-SCE-112409|reactome:R-SCE-112411|reactome:R-SCE-170968|reactome:R-SCE-198753|reactome:R-SCE-202670|reactome:R-SCE-2559582|reactome:R-SCE-3371453|reactome:R-SCE-375165|reactome:R-SCE-437239|reactome:R-SCE-445144|reactome:R-SCE-450341|reactome:R-SCE-5673001|reactome:R-SCE-5674135|reactome:R-SCE-5674499|reactome:R-SCE-5687128|reactome:R-SCE-6798695|reactome:R-SCE-881907|reactome:R-SCE-9634635	refseq:NP_010388.1|sgd:S000002510|go:"GO:0010525"(regulation of transposition, RNA-mediated)|go:"GO:0019900"(kinase binding)|go:"GO:0031681"(G-protein beta-subunit binding)|go:"GO:0043332"(mating projection tip)|go:"GO:0043409"(negative regulation of MAPK cascade)|go:"GO:0070867"(mating projection tip membrane)|interpro:IPR021106|interpro:IPR021651|interpro:IPR038382|rcsb pdb:2KGN|rcsb pdb:2L4U|rcsb pdb:3FZE|rcsb pdb:4F2H|go:"GO:0005886"(plasma membrane)|ensemblfungi:YDR103W(gene)|ensemblfungi:YDR103W_mRNA(transcript)|go:"GO:0000750"(pheromone-dependent signal transduction involved in conjugation with cellular fusion)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005546"(phosphatidylinositol-4,5-bisphosphate binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|dip:DIP-858N|mint:P32917	go:"GO:0004672"(protein kinase activity)|go:"GO:0006468"(protein phosphorylation)	crc64:5117980D20A1E7E2	crc64:0435BDA0196B2D6F	resulting-ptm:Phosphorylation, and activation, of Fus3|figure legend:Fig. 2B, Supp. Fig. 2B|comment:Mutation of the two N-terminal MAPK docking sites of Ste7 inhibit this interaction but no details of these mutations are given.|comment:Defective Ste5 mutants are defined as falling below the limit of detection of the assay.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2009/05/11	2014/10/16	rogid:FaPNQ+7PYnYCFIVspwEcRFOgv0w559292	rogid:4AGoihw1xiRnhpu4/VuycJzCLaE559292	intact-crc:549F218E77223613|rigid:88GnNWUrnKNfFdRBO5DHVjBdy0A	false	his tag:n-n	necessary binding region:778-786|sufficient binding region:593-786|mutation:710-710,712-712,714-714|mutation:745-745,747-748|mutation:632-632,628-628,635-635|mutation:750-752|mutation:754-754,756-756|mutation:746-746,744-744|mutation:769-769,765-765|maltose binding protein tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P16892	uniprotkb:P06784	intact:EBI-7193|uniprotkb:D6VPY4|ensemblfungi:YBL016W	intact:EBI-18389|uniprotkb:D6VRJ2|ensemblfungi:YDL159W	psi-mi:fus3_yeast(display_long)|uniprotkb:FUS3(gene name)|psi-mi:FUS3(display_short)|uniprotkb:DAC2(gene name synonym)|uniprotkb:YBL016W(locus name)|uniprotkb:YBL0303(orf name)|uniprotkb:YBL03.21(orf name)	psi-mi:ste7_yeast(display_long)|uniprotkb:STE7(gene name)|psi-mi:STE7(display_short)|uniprotkb:D1525(orf name)|uniprotkb:YDL159W(locus name)	psi-mi:"MI:0424"(protein kinase assay)	Good et al. (2009)	imex:IM-12170|pubmed:19303851	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0217"(phosphorylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2326314|imex:IM-12170-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009537.1|sgd:S000000112|dip:DIP-714N|mint:P16892|ensemblfungi:YBL016W(gene)|ensemblfungi:YBL016W_mRNA(transcript)|go:"GO:0000750"(pheromone-dependent signal transduction involved in conjugation with cellular fusion)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004707"(MAP kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007049"(cell cycle)|go:"GO:0010494"(cytoplasmic stress granule)|go:"GO:0010526"(negative regulation of transposition, RNA-mediated)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042597"(periplasmic space)|go:"GO:0042802"(identical protein binding)|go:"GO:0043332"(mating projection tip)|go:"GO:0043409"(negative regulation of MAPK cascade)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0046827"(positive regulation of protein export from nucleus)|go:"GO:0051301"(cell division)|go:"GO:0071507"(pheromone response MAPK cascade)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR003527(MAP kinase, conserved site)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|rcsb pdb:2B9F|rcsb pdb:2B9H|rcsb pdb:2B9I|rcsb pdb:2B9J|rcsb pdb:2F49|rcsb pdb:2F9G|rcsb pdb:2FA2|reactome:R-SCE-110056|reactome:R-SCE-111995|reactome:R-SCE-112409|reactome:R-SCE-112411|reactome:R-SCE-170968|reactome:R-SCE-198753|reactome:R-SCE-202670|reactome:R-SCE-2559582|reactome:R-SCE-3371453|reactome:R-SCE-375165|reactome:R-SCE-437239|reactome:R-SCE-445144|reactome:R-SCE-450341|reactome:R-SCE-5673001|reactome:R-SCE-5674135|reactome:R-SCE-5674499|reactome:R-SCE-5687128|reactome:R-SCE-6798695|reactome:R-SCE-881907|reactome:R-SCE-9634635	refseq:NP_010122.1|sgd:S000002318|reactome:R-SCE-112411|reactome:R-SCE-445144|reactome:R-SCE-5674135|reactome:R-SCE-5674499|ensemblfungi:YDL159W(gene)|ensemblfungi:YDL159W_mRNA(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0000196"(cell wall integrity MAPK cascade)|go:"GO:0001402"(signal transduction involved in filamentous growth)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007124"(pseudohyphal growth)|go:"GO:0010525"(regulation of transposition, RNA-mediated)|go:"GO:0043332"(mating projection tip)|go:"GO:0071507"(pheromone response MAPK cascade)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|reactome:R-SCE-110056|dip:DIP-9N|mint:P06784	go:"GO:0004672"(protein kinase activity)|go:"GO:0006468"(protein phosphorylation)	crc64:5117980D20A1E7E2	crc64:C8A75899CFBE8BDE	resulting-ptm:Phosphorylation, and activation, of Fus3|figure legend:Fig. 2B, Supp. Fig. 2B|comment:Mutation of the two N-terminal MAPK docking sites of Ste7 inhibit this interaction but no details of these mutations are given.|comment:Defective Ste5 mutants are defined as falling below the limit of detection of the assay.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2009/05/11	2014/10/16	rogid:FaPNQ+7PYnYCFIVspwEcRFOgv0w559292	rogid:dyC78MRkFAByOXZMkYmZ8nul+dU559292	intact-crc:549F218E77223613|rigid:88GnNWUrnKNfFdRBO5DHVjBdy0A	false	his tag:n-n	glutathione s tranferase tag:n-n|mutation:359-359,363-363|his tag:c-c	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P06784	uniprotkb:P32917	intact:EBI-18389|uniprotkb:D6VRJ2|ensemblfungi:YDL159W	intact:EBI-18373|uniprotkb:D6VS89|ensemblfungi:YDR103W	psi-mi:ste7_yeast(display_long)|uniprotkb:STE7(gene name)|psi-mi:STE7(display_short)|uniprotkb:D1525(orf name)|uniprotkb:YDL159W(locus name)	psi-mi:ste5_yeast(display_long)|uniprotkb:STE5(gene name)|psi-mi:STE5(display_short)|uniprotkb:NUL3(gene name synonym)|uniprotkb:YDR103W(locus name)|uniprotkb:YD8557.12(orf name)	psi-mi:"MI:0053"(fluorescence polarization spectroscopy)	Good et al. (2009)	imex:IM-12170|pubmed:19303851	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2337095|imex:IM-12170-1	-	-	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_010122.1|sgd:S000002318|reactome:R-SCE-112411|reactome:R-SCE-445144|reactome:R-SCE-5674135|reactome:R-SCE-5674499|ensemblfungi:YDL159W(gene)|ensemblfungi:YDL159W_mRNA(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0000196"(cell wall integrity MAPK cascade)|go:"GO:0001402"(signal transduction involved in filamentous growth)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007124"(pseudohyphal growth)|go:"GO:0010525"(regulation of transposition, RNA-mediated)|go:"GO:0043332"(mating projection tip)|go:"GO:0071507"(pheromone response MAPK cascade)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|reactome:R-SCE-110056|dip:DIP-9N|mint:P06784	refseq:NP_010388.1|sgd:S000002510|go:"GO:0010525"(regulation of transposition, RNA-mediated)|go:"GO:0019900"(kinase binding)|go:"GO:0031681"(G-protein beta-subunit binding)|go:"GO:0043332"(mating projection tip)|go:"GO:0043409"(negative regulation of MAPK cascade)|go:"GO:0070867"(mating projection tip membrane)|interpro:IPR021106|interpro:IPR021651|interpro:IPR038382|rcsb pdb:2KGN|rcsb pdb:2L4U|rcsb pdb:3FZE|rcsb pdb:4F2H|go:"GO:0005886"(plasma membrane)|ensemblfungi:YDR103W(gene)|ensemblfungi:YDR103W_mRNA(transcript)|go:"GO:0000750"(pheromone-dependent signal transduction involved in conjugation with cellular fusion)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005546"(phosphatidylinositol-4,5-bisphosphate binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|dip:DIP-858N|mint:P32917	psi-mi:"MI:0469"(intact)	crc64:C8A75899CFBE8BDE	crc64:0435BDA0196B2D6F	figure legend:Fig. 3F|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2009/05/11	2014/10/16	rogid:dyC78MRkFAByOXZMkYmZ8nul+dU559292	rogid:4AGoihw1xiRnhpu4/VuycJzCLaE559292	intact-crc:D97421A90E487A46|rigid:gtEoqcBVpwl+M5VAwVak6f5qAyU	false	glutathione s tranferase tag:n-n|his tag:c-c	tag:?-?|sufficient binding region:593-786	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P16892	uniprotkb:P32917	intact:EBI-7193|uniprotkb:D6VPY4|ensemblfungi:YBL016W	intact:EBI-18373|uniprotkb:D6VS89|ensemblfungi:YDR103W	psi-mi:fus3_yeast(display_long)|uniprotkb:FUS3(gene name)|psi-mi:FUS3(display_short)|uniprotkb:DAC2(gene name synonym)|uniprotkb:YBL016W(locus name)|uniprotkb:YBL0303(orf name)|uniprotkb:YBL03.21(orf name)	psi-mi:ste5_yeast(display_long)|uniprotkb:STE5(gene name)|psi-mi:STE5(display_short)|uniprotkb:NUL3(gene name synonym)|uniprotkb:YDR103W(locus name)|uniprotkb:YD8557.12(orf name)	psi-mi:"MI:0424"(protein kinase assay)	Good et al. (2009)	imex:IM-12170|pubmed:19303851	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0217"(phosphorylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2337113|imex:IM-12170-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0684"(ancillary)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009537.1|sgd:S000000112|dip:DIP-714N|mint:P16892|ensemblfungi:YBL016W(gene)|ensemblfungi:YBL016W_mRNA(transcript)|go:"GO:0000750"(pheromone-dependent signal transduction involved in conjugation with cellular fusion)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004707"(MAP kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007049"(cell cycle)|go:"GO:0010494"(cytoplasmic stress granule)|go:"GO:0010526"(negative regulation of transposition, RNA-mediated)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042597"(periplasmic space)|go:"GO:0042802"(identical protein binding)|go:"GO:0043332"(mating projection tip)|go:"GO:0043409"(negative regulation of MAPK cascade)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0046827"(positive regulation of protein export from nucleus)|go:"GO:0051301"(cell division)|go:"GO:0071507"(pheromone response MAPK cascade)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR003527(MAP kinase, conserved site)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|rcsb pdb:2B9F|rcsb pdb:2B9H|rcsb pdb:2B9I|rcsb pdb:2B9J|rcsb pdb:2F49|rcsb pdb:2F9G|rcsb pdb:2FA2|reactome:R-SCE-110056|reactome:R-SCE-111995|reactome:R-SCE-112409|reactome:R-SCE-112411|reactome:R-SCE-170968|reactome:R-SCE-198753|reactome:R-SCE-202670|reactome:R-SCE-2559582|reactome:R-SCE-3371453|reactome:R-SCE-375165|reactome:R-SCE-437239|reactome:R-SCE-445144|reactome:R-SCE-450341|reactome:R-SCE-5673001|reactome:R-SCE-5674135|reactome:R-SCE-5674499|reactome:R-SCE-5687128|reactome:R-SCE-6798695|reactome:R-SCE-881907|reactome:R-SCE-9634635	refseq:NP_010388.1|sgd:S000002510|go:"GO:0010525"(regulation of transposition, RNA-mediated)|go:"GO:0019900"(kinase binding)|go:"GO:0031681"(G-protein beta-subunit binding)|go:"GO:0043332"(mating projection tip)|go:"GO:0043409"(negative regulation of MAPK cascade)|go:"GO:0070867"(mating projection tip membrane)|interpro:IPR021106|interpro:IPR021651|interpro:IPR038382|rcsb pdb:2KGN|rcsb pdb:2L4U|rcsb pdb:3FZE|rcsb pdb:4F2H|go:"GO:0005886"(plasma membrane)|ensemblfungi:YDR103W(gene)|ensemblfungi:YDR103W_mRNA(transcript)|go:"GO:0000750"(pheromone-dependent signal transduction involved in conjugation with cellular fusion)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005546"(phosphatidylinositol-4,5-bisphosphate binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|dip:DIP-858N|mint:P32917	go:"GO:0004672"(protein kinase activity)|go:"GO:0006468"(protein phosphorylation)	crc64:5117980D20A1E7E2	crc64:0435BDA0196B2D6F	resulting-ptm:Phosphorylation, and activation, of Fus3|figure legend:Fig. 4B, 4C, Supp. Figs. 1A, 6B, 7|comment:"Ste7EE-ND2 (which contains only one MAPK docking motif, KD 100nM), and Fus3-K42R (which is catalytically dead) were used to simplify the analyses. Kinase reactions contain 50nM Ste7EE-ND2, and a saturating concentration (1000nM, where appropriate) of truncated Ste5. The Ste5 domain enhances the kcat of Fus3 phosphorylation by Ste7EE by 5000-fold, with little effect on the Km"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2009/05/11	2014/10/16	rogid:FaPNQ+7PYnYCFIVspwEcRFOgv0w559292	rogid:4AGoihw1xiRnhpu4/VuycJzCLaE559292	intact-crc:360789367FAF8EF9|rigid:88GnNWUrnKNfFdRBO5DHVjBdy0A	false	his tag:n-n|mutation:42-42	mutation:744-744,746-746|mutation:747-748,745-745|mutation:750-752|mutation:765-765,769-769|maltose binding protein tag:?-?|sufficient binding region:593-786|necessary binding region:778-786|mutation:756-756,754-754	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P16892	uniprotkb:P06784	intact:EBI-7193|uniprotkb:D6VPY4|ensemblfungi:YBL016W	intact:EBI-18389|uniprotkb:D6VRJ2|ensemblfungi:YDL159W	psi-mi:fus3_yeast(display_long)|uniprotkb:FUS3(gene name)|psi-mi:FUS3(display_short)|uniprotkb:DAC2(gene name synonym)|uniprotkb:YBL016W(locus name)|uniprotkb:YBL0303(orf name)|uniprotkb:YBL03.21(orf name)	psi-mi:ste7_yeast(display_long)|uniprotkb:STE7(gene name)|psi-mi:STE7(display_short)|uniprotkb:D1525(orf name)|uniprotkb:YDL159W(locus name)	psi-mi:"MI:0424"(protein kinase assay)	Good et al. (2009)	imex:IM-12170|pubmed:19303851	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0217"(phosphorylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2337113|imex:IM-12170-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009537.1|sgd:S000000112|dip:DIP-714N|mint:P16892|ensemblfungi:YBL016W(gene)|ensemblfungi:YBL016W_mRNA(transcript)|go:"GO:0000750"(pheromone-dependent signal transduction involved in conjugation with cellular fusion)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004707"(MAP kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007049"(cell cycle)|go:"GO:0010494"(cytoplasmic stress granule)|go:"GO:0010526"(negative regulation of transposition, RNA-mediated)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042597"(periplasmic space)|go:"GO:0042802"(identical protein binding)|go:"GO:0043332"(mating projection tip)|go:"GO:0043409"(negative regulation of MAPK cascade)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0046827"(positive regulation of protein export from nucleus)|go:"GO:0051301"(cell division)|go:"GO:0071507"(pheromone response MAPK cascade)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR003527(MAP kinase, conserved site)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|rcsb pdb:2B9F|rcsb pdb:2B9H|rcsb pdb:2B9I|rcsb pdb:2B9J|rcsb pdb:2F49|rcsb pdb:2F9G|rcsb pdb:2FA2|reactome:R-SCE-110056|reactome:R-SCE-111995|reactome:R-SCE-112409|reactome:R-SCE-112411|reactome:R-SCE-170968|reactome:R-SCE-198753|reactome:R-SCE-202670|reactome:R-SCE-2559582|reactome:R-SCE-3371453|reactome:R-SCE-375165|reactome:R-SCE-437239|reactome:R-SCE-445144|reactome:R-SCE-450341|reactome:R-SCE-5673001|reactome:R-SCE-5674135|reactome:R-SCE-5674499|reactome:R-SCE-5687128|reactome:R-SCE-6798695|reactome:R-SCE-881907|reactome:R-SCE-9634635	refseq:NP_010122.1|sgd:S000002318|reactome:R-SCE-112411|reactome:R-SCE-445144|reactome:R-SCE-5674135|reactome:R-SCE-5674499|ensemblfungi:YDL159W(gene)|ensemblfungi:YDL159W_mRNA(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0000196"(cell wall integrity MAPK cascade)|go:"GO:0001402"(signal transduction involved in filamentous growth)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007124"(pseudohyphal growth)|go:"GO:0010525"(regulation of transposition, RNA-mediated)|go:"GO:0043332"(mating projection tip)|go:"GO:0071507"(pheromone response MAPK cascade)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|reactome:R-SCE-110056|dip:DIP-9N|mint:P06784	go:"GO:0004672"(protein kinase activity)|go:"GO:0006468"(protein phosphorylation)	crc64:5117980D20A1E7E2	crc64:C8A75899CFBE8BDE	resulting-ptm:Phosphorylation, and activation, of Fus3|figure legend:Fig. 4B, 4C, Supp. Figs. 1A, 6B, 7|comment:"Ste7EE-ND2 (which contains only one MAPK docking motif, KD 100nM), and Fus3-K42R (which is catalytically dead) were used to simplify the analyses. Kinase reactions contain 50nM Ste7EE-ND2, and a saturating concentration (1000nM, where appropriate) of truncated Ste5. The Ste5 domain enhances the kcat of Fus3 phosphorylation by Ste7EE by 5000-fold, with little effect on the Km"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2009/05/11	2014/10/16	rogid:FaPNQ+7PYnYCFIVspwEcRFOgv0w559292	rogid:dyC78MRkFAByOXZMkYmZ8nul+dU559292	intact-crc:360789367FAF8EF9|rigid:88GnNWUrnKNfFdRBO5DHVjBdy0A	false	his tag:n-n|mutation:42-42	glutathione s tranferase tag:n-n|mutation:359-359,363-363|his tag:c-c	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P14681	uniprotkb:P06784	intact:EBI-9945|uniprotkb:Q45U43|uniprotkb:D6VUH8|ensemblfungi:YGR040W	intact:EBI-18389|uniprotkb:D6VRJ2|ensemblfungi:YDL159W	psi-mi:kss1_yeast(display_long)|uniprotkb:KSS1(gene name)|psi-mi:KSS1(display_short)|uniprotkb:Kinase suppressor of SST2(gene name synonym)|uniprotkb:YGR040W(locus name)	psi-mi:ste7_yeast(display_long)|uniprotkb:STE7(gene name)|psi-mi:STE7(display_short)|uniprotkb:D1525(orf name)|uniprotkb:YDL159W(locus name)	psi-mi:"MI:0424"(protein kinase assay)	Good et al. (2009)	imex:IM-12170|pubmed:19303851	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0217"(phosphorylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2337174|imex:IM-12170-5	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000003272|ensemblfungi:YGR040W(gene)|ensemblfungi:YGR040W_mRNA(transcript)|go:"GO:0000750"(pheromone-dependent signal transduction involved in conjugation with cellular fusion)|go:"GO:0001402"(signal transduction involved in filamentous growth)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004707"(MAP kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005935"(cellular bud neck)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007049"(cell cycle)|go:"GO:0008134"(transcription factor binding)|go:"GO:0010973"(positive regulation of division septum assembly)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042597"(periplasmic space)|go:"GO:0043433"(negative regulation of DNA-binding transcription factor activity)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR003527(MAP kinase, conserved site)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|reactome:R-SCE-111995|reactome:R-SCE-112411|reactome:R-SCE-198753|reactome:R-SCE-202670|reactome:R-SCE-2559582|reactome:R-SCE-375165|reactome:R-SCE-437239|reactome:R-SCE-5687128|reactome:R-SCE-6798695|reactome:R-SCE-881907|reactome:R-SCE-9634635|refseq:NP_011554.3|reactome:R-SCE-112409|reactome:R-SCE-3371453|reactome:R-SCE-445144|reactome:R-SCE-5673001|reactome:R-SCE-5674135|reactome:R-SCE-5674499|dip:DIP-60N|reactome:R-SCE-170968|reactome:R-SCE-450341	refseq:NP_010122.1|sgd:S000002318|reactome:R-SCE-112411|reactome:R-SCE-445144|reactome:R-SCE-5674135|reactome:R-SCE-5674499|ensemblfungi:YDL159W(gene)|ensemblfungi:YDL159W_mRNA(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0000196"(cell wall integrity MAPK cascade)|go:"GO:0001402"(signal transduction involved in filamentous growth)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007124"(pseudohyphal growth)|go:"GO:0010525"(regulation of transposition, RNA-mediated)|go:"GO:0043332"(mating projection tip)|go:"GO:0071507"(pheromone response MAPK cascade)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|reactome:R-SCE-110056|dip:DIP-9N|mint:P06784	go:"GO:0006468"(protein phosphorylation)|go:"GO:0004672"(protein kinase activity)	crc64:5FA42161B5FD51B5	crc64:C8A75899CFBE8BDE	resulting-ptm:Phosphorylation, and activation, of KSS1|figure legend:Figs. 4B, Supp. Fig. 2A|comment:Michaelis-Menten plots showed Ste7 phosphorylation of Kss1 does not require the presence of Ste5, and had no effect of the km or Kcat of the reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2009/05/11	2014/10/16	rogid:xE1y1jku+iWy4xzv9wUCul1AFZs559292	rogid:dyC78MRkFAByOXZMkYmZ8nul+dU559292	intact-crc:9C6C02E29337781A|rigid:v9VALaELwEARboEE7iHaBr1KUi0	false	his tag:n-n	glutathione s tranferase tag:n-n|mutation:359-359,363-363|his tag:c-c	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)