#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P47142 uniprotkb:Q04272 intact:EBI-25595|uniprotkb:D6VWS1|ensemblfungi:YJR102C intact:EBI-28157|uniprotkb:D6VZQ1|ensemblfungi:YMR077C psi-mi:vps25_yeast(display_long)|uniprotkb:YJR102C(locus name)|uniprotkb:J1957(orf name)|uniprotkb:VPS25(gene name)|psi-mi:VPS25(display_short)|uniprotkb:VPT25(gene name synonym)|uniprotkb:ESCRT-II complex subunit VPS25(gene name synonym) psi-mi:vps20_yeast(display_long)|uniprotkb:ASI10(gene name synonym)|uniprotkb:CHM6(gene name synonym)|uniprotkb:VPT20(gene name synonym)|uniprotkb:YM9582.02C(orf name)|uniprotkb:YMR077C(locus name)|uniprotkb:VPS20(gene name)|psi-mi:VPS20(display_short)|uniprotkb:Vacuolar protein-targeting protein 20(gene name synonym)|uniprotkb:Amino acid sensor-independent protein 10(gene name synonym)|uniprotkb:Charged multivesicular body protein 6(gene name synonym)|uniprotkb:ESCRT-III complex subunit VPS20(gene name synonym) psi-mi:"MI:0051"(fluorescence technology) Saksena et al. (2009) imex:IM-12158|pubmed:19135892 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-2213948|imex:IM-12158-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_012636.1|sgd:S000003863|mint:P47142|dip:DIP-4446N|ensemblfungi:YJR102C(gene)|ensemblfungi:YJR102C_mRNA(transcript)|go:"GO:0000433"(carbon catabolite repression of transcription from RNA polymerase II promoter by glucose)|go:"GO:0000814"(ESCRT II complex)|go:"GO:0005198"(structural molecule activity)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043162"(ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)|go:"GO:0043328"(protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)|go:"GO:1904669"(ATP export)|interpro:IPR008570(ESCRT-II complex, vps25 subunit)|interpro:IPR014041(ESCRT-II complex, vps25 subunit, N-terminal winged helix)|interpro:IPR036388|interpro:IPR036390|rcsb pdb:1U5T|rcsb pdb:1W7P|rcsb pdb:1XB4|reactome:R-SCE-917729 reactome:R-SCE-1632852|refseq:NP_013794.1|sgd:S000004682|dip:DIP-1961N|reactome:R-SCE-917729|reactome:R-SCE-9668328|ensemblfungi:YMR077C(gene)|ensemblfungi:YMR077C_mRNA(transcript)|go:"GO:0000815"(ESCRT III complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0005771"(multivesicular body)|go:"GO:0005774"(vacuolar membrane)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005829"(cytosol)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0015031"(protein transport)|go:"GO:0032511"(late endosome to vacuole transport via multivesicular body sorting pathway)|go:"GO:0043162"(ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)|go:"GO:0045324"(late endosome to vacuole transport)|go:"GO:0070676"(intralumenal vesicle formation)|go:"GO:1904669"(ATP export)|interpro:IPR005024(Snf7)|rcsb pdb:5FVL go:"GO:0016020"(membrane) crc64:6A4545A8BC772CA2 crc64:CDC88BC4ADA3D602 comment:"A fluorescent 7-nitrobenz-2-oxa-1,3-diazole (NBD) dye was covalently attached to the Cys mutated into the protein sequence."|comment:Vps20 must be membrane-bound for interaction to occur.|figure legend:Figs. 2D-2F, Supp. Fig. 4|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2009/01/28 2014/10/16 rogid:LlNZVpWKYsWsgu0FcP2sL10lYHc559292 rogid:K6P+RVrruRMbHsMdRoH/v0f+0Z0559292 intact-crc:B38804081C3BE84B|rigid:68F7OE3dD3vF5HibUCQM7ofe8Po false - mutation:61-61 - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P39929 uniprotkb:Q04272 intact:EBI-17554|uniprotkb:D6VY27|ensemblfungi:YLR025W|uniprotkb:E9P8V6 intact:EBI-28157|uniprotkb:D6VZQ1|ensemblfungi:YMR077C psi-mi:snf7_yeast(display_long)|uniprotkb:SNF7(gene name)|psi-mi:SNF7(display_short)|uniprotkb:VPS32(gene name synonym)|uniprotkb:YLR025W(locus name)|uniprotkb:DID1(gene name synonym)|uniprotkb:Vacuolar protein-sorting-associated protein 32(gene name synonym)|uniprotkb:DOA4-independent degradation protein 1(gene name synonym)|uniprotkb:Sucrose nonfermenting protein 7(gene name synonym) psi-mi:vps20_yeast(display_long)|uniprotkb:ASI10(gene name synonym)|uniprotkb:CHM6(gene name synonym)|uniprotkb:VPT20(gene name synonym)|uniprotkb:YM9582.02C(orf name)|uniprotkb:YMR077C(locus name)|uniprotkb:VPS20(gene name)|psi-mi:VPS20(display_short)|uniprotkb:Vacuolar protein-targeting protein 20(gene name synonym)|uniprotkb:Amino acid sensor-independent protein 10(gene name synonym)|uniprotkb:Charged multivesicular body protein 6(gene name synonym)|uniprotkb:ESCRT-III complex subunit VPS20(gene name synonym) psi-mi:"MI:0051"(fluorescence technology) Saksena et al. (2009) imex:IM-12158|pubmed:19135892 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-2213958|imex:IM-12158-2 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_013125.1|sgd:S000004015|ensemblfungi:YLR025W(gene)|ensemblfungi:YLR025W_mRNA(transcript)|go:"GO:0000815"(ESCRT III complex)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005737"(cytoplasm)|go:"GO:0005771"(multivesicular body)|go:"GO:0005886"(plasma membrane)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0015031"(protein transport)|go:"GO:0042802"(identical protein binding)|go:"GO:0043162"(ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)|go:"GO:0045324"(late endosome to vacuole transport)|go:"GO:0061709"(reticulophagy)|go:"GO:0032511"(late endosome to vacuole transport via multivesicular body sorting pathway)|go:"GO:0070676"(intralumenal vesicle formation)|go:"GO:0071454"(cellular response to anoxia)|go:"GO:1904669"(ATP export)|go:"GO:1904902"(ESCRT III complex assembly)|interpro:IPR005024(Snf7)|rcsb pdb:5FD7|rcsb pdb:5FD9|rcsb pdb:5T8L|rcsb pdb:5T8N|reactome:R-SCE-1632852|reactome:R-SCE-917729|reactome:R-SCE-9668328|dip:DIP-1747N|mint:P39929 reactome:R-SCE-1632852|refseq:NP_013794.1|sgd:S000004682|dip:DIP-1961N|reactome:R-SCE-917729|reactome:R-SCE-9668328|ensemblfungi:YMR077C(gene)|ensemblfungi:YMR077C_mRNA(transcript)|go:"GO:0000815"(ESCRT III complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0005771"(multivesicular body)|go:"GO:0005774"(vacuolar membrane)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005829"(cytosol)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0015031"(protein transport)|go:"GO:0032511"(late endosome to vacuole transport via multivesicular body sorting pathway)|go:"GO:0043162"(ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)|go:"GO:0045324"(late endosome to vacuole transport)|go:"GO:0070676"(intralumenal vesicle formation)|go:"GO:1904669"(ATP export)|interpro:IPR005024(Snf7)|rcsb pdb:5FVL psi-mi:"MI:0469"(intact) crc64:5241A18BB181F0C1 crc64:CDC88BC4ADA3D602 comment:"A fluorescent 7-nitrobenz-2-oxa-1,3-diazole (NBD) dye was covalently attached to the Cys mutated into the protein sequence."|comment:Vps20 binds to both free and membrane-bound Snf7.|figure legend:Fig. 4, Supp. Fig. 7|comment:A probe at postion 200 of SNF7 was sensitive to membrane binding but not VPS20 binding.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2009/01/28 2014/10/16 rogid:I8qBtNlqTjxLX1HginTOzM3o9oA559292 rogid:K6P+RVrruRMbHsMdRoH/v0f+0Z0559292 intact-crc:35452771D69BA196|rigid:4h2JBFD8dsB++Iu44rwXpi5Xk74 false mutation:58-58 - - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P39929 uniprotkb:P39929 intact:EBI-17554|uniprotkb:D6VY27|ensemblfungi:YLR025W|uniprotkb:E9P8V6 intact:EBI-17554|uniprotkb:D6VY27|ensemblfungi:YLR025W|uniprotkb:E9P8V6 psi-mi:snf7_yeast(display_long)|uniprotkb:SNF7(gene name)|psi-mi:SNF7(display_short)|uniprotkb:VPS32(gene name synonym)|uniprotkb:YLR025W(locus name)|uniprotkb:DID1(gene name synonym)|uniprotkb:Vacuolar protein-sorting-associated protein 32(gene name synonym)|uniprotkb:DOA4-independent degradation protein 1(gene name synonym)|uniprotkb:Sucrose nonfermenting protein 7(gene name synonym) psi-mi:snf7_yeast(display_long)|uniprotkb:SNF7(gene name)|psi-mi:SNF7(display_short)|uniprotkb:VPS32(gene name synonym)|uniprotkb:YLR025W(locus name)|uniprotkb:DID1(gene name synonym)|uniprotkb:Vacuolar protein-sorting-associated protein 32(gene name synonym)|uniprotkb:DOA4-independent degradation protein 1(gene name synonym)|uniprotkb:Sucrose nonfermenting protein 7(gene name synonym) psi-mi:"MI:0055"(fluorescent resonance energy transfer) Saksena et al. (2009) imex:IM-12158|pubmed:19135892 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-2213976|imex:IM-12158-5 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0584"(fluorescence acceptor) psi-mi:"MI:0583"(fluorescence donor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_013125.1|sgd:S000004015|ensemblfungi:YLR025W(gene)|ensemblfungi:YLR025W_mRNA(transcript)|go:"GO:0000815"(ESCRT III complex)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005737"(cytoplasm)|go:"GO:0005771"(multivesicular body)|go:"GO:0005886"(plasma membrane)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0015031"(protein transport)|go:"GO:0042802"(identical protein binding)|go:"GO:0043162"(ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)|go:"GO:0045324"(late endosome to vacuole transport)|go:"GO:0061709"(reticulophagy)|go:"GO:0032511"(late endosome to vacuole transport via multivesicular body sorting pathway)|go:"GO:0070676"(intralumenal vesicle formation)|go:"GO:0071454"(cellular response to anoxia)|go:"GO:1904669"(ATP export)|go:"GO:1904902"(ESCRT III complex assembly)|interpro:IPR005024(Snf7)|rcsb pdb:5FD7|rcsb pdb:5FD9|rcsb pdb:5T8L|rcsb pdb:5T8N|reactome:R-SCE-1632852|reactome:R-SCE-917729|reactome:R-SCE-9668328|dip:DIP-1747N|mint:P39929 refseq:NP_013125.1|sgd:S000004015|ensemblfungi:YLR025W(gene)|ensemblfungi:YLR025W_mRNA(transcript)|go:"GO:0000815"(ESCRT III complex)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005737"(cytoplasm)|go:"GO:0005771"(multivesicular body)|go:"GO:0005886"(plasma membrane)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0015031"(protein transport)|go:"GO:0042802"(identical protein binding)|go:"GO:0043162"(ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)|go:"GO:0045324"(late endosome to vacuole transport)|go:"GO:0061709"(reticulophagy)|go:"GO:0032511"(late endosome to vacuole transport via multivesicular body sorting pathway)|go:"GO:0070676"(intralumenal vesicle formation)|go:"GO:0071454"(cellular response to anoxia)|go:"GO:1904669"(ATP export)|go:"GO:1904902"(ESCRT III complex assembly)|interpro:IPR005024(Snf7)|rcsb pdb:5FD7|rcsb pdb:5FD9|rcsb pdb:5T8L|rcsb pdb:5T8N|reactome:R-SCE-1632852|reactome:R-SCE-917729|reactome:R-SCE-9668328|dip:DIP-1747N|mint:P39929 go:"GO:0016020"(membrane) crc64:5241A18BB181F0C1 crc64:5241A18BB181F0C1 figure legend:Figs. 5A, 5B, Supp. Table 1|comment:Both membranes and Vps20 are required for maximal association of Snf7 molecules.|stimulant:"Vps20 (Q04272) and liposomes"|comment:"A fluorescent 7-nitrobenz-2-oxa-1,3-diazole (NBD) dye was covalently attached to the Cys mutated into the protein sequence."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2009/01/28 2014/10/16 rogid:I8qBtNlqTjxLX1HginTOzM3o9oA559292 rogid:I8qBtNlqTjxLX1HginTOzM3o9oA559292 intact-crc:CEE9C18919945851|rigid:CuP2MR5p8lfZPJWeY83YiHHsYGM false fluorescent dye label:81-81 mutation:81-81|fluorescent dye label:81-81 - 1 psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P39929 uniprotkb:P39929 intact:EBI-17554|uniprotkb:D6VY27|ensemblfungi:YLR025W|uniprotkb:E9P8V6 intact:EBI-17554|uniprotkb:D6VY27|ensemblfungi:YLR025W|uniprotkb:E9P8V6 psi-mi:snf7_yeast(display_long)|uniprotkb:SNF7(gene name)|psi-mi:SNF7(display_short)|uniprotkb:VPS32(gene name synonym)|uniprotkb:YLR025W(locus name)|uniprotkb:DID1(gene name synonym)|uniprotkb:Vacuolar protein-sorting-associated protein 32(gene name synonym)|uniprotkb:DOA4-independent degradation protein 1(gene name synonym)|uniprotkb:Sucrose nonfermenting protein 7(gene name synonym) psi-mi:snf7_yeast(display_long)|uniprotkb:SNF7(gene name)|psi-mi:SNF7(display_short)|uniprotkb:VPS32(gene name synonym)|uniprotkb:YLR025W(locus name)|uniprotkb:DID1(gene name synonym)|uniprotkb:Vacuolar protein-sorting-associated protein 32(gene name synonym)|uniprotkb:DOA4-independent degradation protein 1(gene name synonym)|uniprotkb:Sucrose nonfermenting protein 7(gene name synonym) psi-mi:"MI:0055"(fluorescent resonance energy transfer) Saksena et al. (2009) imex:IM-12158|pubmed:19135892 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-2213989|imex:IM-12158-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0584"(fluorescence acceptor) psi-mi:"MI:0583"(fluorescence donor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_013125.1|sgd:S000004015|ensemblfungi:YLR025W(gene)|ensemblfungi:YLR025W_mRNA(transcript)|go:"GO:0000815"(ESCRT III complex)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005737"(cytoplasm)|go:"GO:0005771"(multivesicular body)|go:"GO:0005886"(plasma membrane)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0015031"(protein transport)|go:"GO:0042802"(identical protein binding)|go:"GO:0043162"(ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)|go:"GO:0045324"(late endosome to vacuole transport)|go:"GO:0061709"(reticulophagy)|go:"GO:0032511"(late endosome to vacuole transport via multivesicular body sorting pathway)|go:"GO:0070676"(intralumenal vesicle formation)|go:"GO:0071454"(cellular response to anoxia)|go:"GO:1904669"(ATP export)|go:"GO:1904902"(ESCRT III complex assembly)|interpro:IPR005024(Snf7)|rcsb pdb:5FD7|rcsb pdb:5FD9|rcsb pdb:5T8L|rcsb pdb:5T8N|reactome:R-SCE-1632852|reactome:R-SCE-917729|reactome:R-SCE-9668328|dip:DIP-1747N|mint:P39929 refseq:NP_013125.1|sgd:S000004015|ensemblfungi:YLR025W(gene)|ensemblfungi:YLR025W_mRNA(transcript)|go:"GO:0000815"(ESCRT III complex)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005737"(cytoplasm)|go:"GO:0005771"(multivesicular body)|go:"GO:0005886"(plasma membrane)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0015031"(protein transport)|go:"GO:0042802"(identical protein binding)|go:"GO:0043162"(ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)|go:"GO:0045324"(late endosome to vacuole transport)|go:"GO:0061709"(reticulophagy)|go:"GO:0032511"(late endosome to vacuole transport via multivesicular body sorting pathway)|go:"GO:0070676"(intralumenal vesicle formation)|go:"GO:0071454"(cellular response to anoxia)|go:"GO:1904669"(ATP export)|go:"GO:1904902"(ESCRT III complex assembly)|interpro:IPR005024(Snf7)|rcsb pdb:5FD7|rcsb pdb:5FD9|rcsb pdb:5T8L|rcsb pdb:5T8N|reactome:R-SCE-1632852|reactome:R-SCE-917729|reactome:R-SCE-9668328|dip:DIP-1747N|mint:P39929 go:"GO:0016020"(membrane) crc64:5241A18BB181F0C1 crc64:5241A18BB181F0C1 figure legend:Figs. 5A, 5B, Supp. Table 1|comment:Both membranes and Vps20 are required for maximal association of Snf7 molecules.|stimulant:"Vps20 (Q04272) and liposomes"|comment:"A fluorescent 7-nitrobenz-2-oxa-1,3-diazole (NBD) dye was covalently attached to the Cys mutated into the protein sequence."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2009/01/28 2014/10/16 rogid:I8qBtNlqTjxLX1HginTOzM3o9oA559292 rogid:I8qBtNlqTjxLX1HginTOzM3o9oA559292 intact-crc:A02B86A91813B0DB|rigid:CuP2MR5p8lfZPJWeY83YiHHsYGM false fluorescent dye label:81-81 fluorescent dye label:21-21|mutation:21-21 - 1 psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P39929 uniprotkb:P39929 intact:EBI-17554|uniprotkb:D6VY27|ensemblfungi:YLR025W|uniprotkb:E9P8V6 intact:EBI-17554|uniprotkb:D6VY27|ensemblfungi:YLR025W|uniprotkb:E9P8V6 psi-mi:snf7_yeast(display_long)|uniprotkb:SNF7(gene name)|psi-mi:SNF7(display_short)|uniprotkb:VPS32(gene name synonym)|uniprotkb:YLR025W(locus name)|uniprotkb:DID1(gene name synonym)|uniprotkb:Vacuolar protein-sorting-associated protein 32(gene name synonym)|uniprotkb:DOA4-independent degradation protein 1(gene name synonym)|uniprotkb:Sucrose nonfermenting protein 7(gene name synonym) psi-mi:snf7_yeast(display_long)|uniprotkb:SNF7(gene name)|psi-mi:SNF7(display_short)|uniprotkb:VPS32(gene name synonym)|uniprotkb:YLR025W(locus name)|uniprotkb:DID1(gene name synonym)|uniprotkb:Vacuolar protein-sorting-associated protein 32(gene name synonym)|uniprotkb:DOA4-independent degradation protein 1(gene name synonym)|uniprotkb:Sucrose nonfermenting protein 7(gene name synonym) psi-mi:"MI:0055"(fluorescent resonance energy transfer) Saksena et al. (2009) imex:IM-12158|pubmed:19135892 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-2214002|imex:IM-12158-4 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0584"(fluorescence acceptor) psi-mi:"MI:0583"(fluorescence donor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_013125.1|sgd:S000004015|ensemblfungi:YLR025W(gene)|ensemblfungi:YLR025W_mRNA(transcript)|go:"GO:0000815"(ESCRT III complex)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005737"(cytoplasm)|go:"GO:0005771"(multivesicular body)|go:"GO:0005886"(plasma membrane)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0015031"(protein transport)|go:"GO:0042802"(identical protein binding)|go:"GO:0043162"(ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)|go:"GO:0045324"(late endosome to vacuole transport)|go:"GO:0061709"(reticulophagy)|go:"GO:0032511"(late endosome to vacuole transport via multivesicular body sorting pathway)|go:"GO:0070676"(intralumenal vesicle formation)|go:"GO:0071454"(cellular response to anoxia)|go:"GO:1904669"(ATP export)|go:"GO:1904902"(ESCRT III complex assembly)|interpro:IPR005024(Snf7)|rcsb pdb:5FD7|rcsb pdb:5FD9|rcsb pdb:5T8L|rcsb pdb:5T8N|reactome:R-SCE-1632852|reactome:R-SCE-917729|reactome:R-SCE-9668328|dip:DIP-1747N|mint:P39929 refseq:NP_013125.1|sgd:S000004015|ensemblfungi:YLR025W(gene)|ensemblfungi:YLR025W_mRNA(transcript)|go:"GO:0000815"(ESCRT III complex)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005737"(cytoplasm)|go:"GO:0005771"(multivesicular body)|go:"GO:0005886"(plasma membrane)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0015031"(protein transport)|go:"GO:0042802"(identical protein binding)|go:"GO:0043162"(ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)|go:"GO:0045324"(late endosome to vacuole transport)|go:"GO:0061709"(reticulophagy)|go:"GO:0032511"(late endosome to vacuole transport via multivesicular body sorting pathway)|go:"GO:0070676"(intralumenal vesicle formation)|go:"GO:0071454"(cellular response to anoxia)|go:"GO:1904669"(ATP export)|go:"GO:1904902"(ESCRT III complex assembly)|interpro:IPR005024(Snf7)|rcsb pdb:5FD7|rcsb pdb:5FD9|rcsb pdb:5T8L|rcsb pdb:5T8N|reactome:R-SCE-1632852|reactome:R-SCE-917729|reactome:R-SCE-9668328|dip:DIP-1747N|mint:P39929 go:"GO:0016020"(membrane) crc64:5241A18BB181F0C1 crc64:5241A18BB181F0C1 figure legend:Figs. 5A, 5B, Supp. Table 1|comment:Both membranes and Vps20 are required for maximal association of Snf7 molecules.|stimulant:"Vps20 (Q04272) and liposomes"|comment:"A fluorescent 7-nitrobenz-2-oxa-1,3-diazole (NBD) dye was covalently attached to the Cys mutated into the protein sequence."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2009/01/28 2014/10/16 rogid:I8qBtNlqTjxLX1HginTOzM3o9oA559292 rogid:I8qBtNlqTjxLX1HginTOzM3o9oA559292 intact-crc:7F7F2BBE4E1E852F|rigid:CuP2MR5p8lfZPJWeY83YiHHsYGM false fluorescent dye label:21-21 mutation:21-21|fluorescent dye label:21-21 - 1 psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P39929 uniprotkb:P39929 intact:EBI-17554|uniprotkb:D6VY27|ensemblfungi:YLR025W|uniprotkb:E9P8V6 intact:EBI-17554|uniprotkb:D6VY27|ensemblfungi:YLR025W|uniprotkb:E9P8V6 psi-mi:snf7_yeast(display_long)|uniprotkb:SNF7(gene name)|psi-mi:SNF7(display_short)|uniprotkb:VPS32(gene name synonym)|uniprotkb:YLR025W(locus name)|uniprotkb:DID1(gene name synonym)|uniprotkb:Vacuolar protein-sorting-associated protein 32(gene name synonym)|uniprotkb:DOA4-independent degradation protein 1(gene name synonym)|uniprotkb:Sucrose nonfermenting protein 7(gene name synonym) psi-mi:snf7_yeast(display_long)|uniprotkb:SNF7(gene name)|psi-mi:SNF7(display_short)|uniprotkb:VPS32(gene name synonym)|uniprotkb:YLR025W(locus name)|uniprotkb:DID1(gene name synonym)|uniprotkb:Vacuolar protein-sorting-associated protein 32(gene name synonym)|uniprotkb:DOA4-independent degradation protein 1(gene name synonym)|uniprotkb:Sucrose nonfermenting protein 7(gene name synonym) psi-mi:"MI:0029"(cosedimentation through density gradient) Saksena et al. (2009) imex:IM-12158|pubmed:19135892 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0469"(IntAct) intact:EBI-2214022|imex:IM-12158-6 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_013125.1|sgd:S000004015|ensemblfungi:YLR025W(gene)|ensemblfungi:YLR025W_mRNA(transcript)|go:"GO:0000815"(ESCRT III complex)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005737"(cytoplasm)|go:"GO:0005771"(multivesicular body)|go:"GO:0005886"(plasma membrane)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0015031"(protein transport)|go:"GO:0042802"(identical protein binding)|go:"GO:0043162"(ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)|go:"GO:0045324"(late endosome to vacuole transport)|go:"GO:0061709"(reticulophagy)|go:"GO:0032511"(late endosome to vacuole transport via multivesicular body sorting pathway)|go:"GO:0070676"(intralumenal vesicle formation)|go:"GO:0071454"(cellular response to anoxia)|go:"GO:1904669"(ATP export)|go:"GO:1904902"(ESCRT III complex assembly)|interpro:IPR005024(Snf7)|rcsb pdb:5FD7|rcsb pdb:5FD9|rcsb pdb:5T8L|rcsb pdb:5T8N|reactome:R-SCE-1632852|reactome:R-SCE-917729|reactome:R-SCE-9668328|dip:DIP-1747N|mint:P39929 refseq:NP_013125.1|sgd:S000004015|ensemblfungi:YLR025W(gene)|ensemblfungi:YLR025W_mRNA(transcript)|go:"GO:0000815"(ESCRT III complex)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005737"(cytoplasm)|go:"GO:0005771"(multivesicular body)|go:"GO:0005886"(plasma membrane)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0015031"(protein transport)|go:"GO:0042802"(identical protein binding)|go:"GO:0043162"(ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway)|go:"GO:0045324"(late endosome to vacuole transport)|go:"GO:0061709"(reticulophagy)|go:"GO:0032511"(late endosome to vacuole transport via multivesicular body sorting pathway)|go:"GO:0070676"(intralumenal vesicle formation)|go:"GO:0071454"(cellular response to anoxia)|go:"GO:1904669"(ATP export)|go:"GO:1904902"(ESCRT III complex assembly)|interpro:IPR005024(Snf7)|rcsb pdb:5FD7|rcsb pdb:5FD9|rcsb pdb:5T8L|rcsb pdb:5T8N|reactome:R-SCE-1632852|reactome:R-SCE-917729|reactome:R-SCE-9668328|dip:DIP-1747N|mint:P39929 go:"GO:0016020"(membrane) crc64:5241A18BB181F0C1 crc64:5241A18BB181F0C1 figure legend:Fig. 5C|comment:Both membranes and Vps20 are required for maximal association of Snf7 molecules.|stimulant:"Vps20 (Q04272) and liposomes"|inhibition:"Vps24 (P36095)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2009/01/28 2014/10/16 rogid:I8qBtNlqTjxLX1HginTOzM3o9oA559292 rogid:I8qBtNlqTjxLX1HginTOzM3o9oA559292 intact-crc:9DF62791D8BCD74A|rigid:dwyiGZRNiBXFuEw6RMunwPOPlCY false mutation decreasing interaction:200-200,203-203,191-191 mutation decreasing interaction:200-200,203-203,191-191 - 0 psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining)