#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P28496 uniprotkb:P38703 intact:EBI-26585|uniprotkb:D6VXS8|ensemblfungi:YKL008C intact:EBI-10035|uniprotkb:Q6LD26|uniprotkb:D3DKR1|ensemblfungi:YHL003C psi-mi:lac1_yeast(display_long)|uniprotkb:LAC1(gene name)|psi-mi:LAC1(display_short)|uniprotkb:DGT1(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAC1(gene name synonym)|uniprotkb:YKL156(orf name)|uniprotkb:YKL008C(locus name) psi-mi:lag1_yeast(display_long)|uniprotkb:LAG1(gene name)|psi-mi:LAG1(display_short)|uniprotkb:Longevity assurance factor 1(gene name synonym)|uniprotkb:Longevity assurance protein 1(gene name synonym)|uniprotkb:Longevity assurance gene 1 protein(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAG1(gene name synonym)|uniprotkb:YHL003C(locus name) psi-mi:"MI:0415"(enzymatic study) Vallee et al. (2005) pubmed:15692566|imex:IM-19081 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0414"(enzymatic reaction) psi-mi:"MI:0469"(IntAct) intact:EBI-2355064|imex:IM-19081-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) sgd:S000001491|refseq:NP_012917.3|mint:P28496|dip:DIP-4348N|ensemblfungi:YKL008C(gene)|ensemblfungi:YKL008C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0034399"(nuclear periphery)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661 refseq:NP_011860.1|sgd:S000000995|ensemblfungi:YHL003C(gene)|ensemblfungi:YHL003C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661|dip:DIP-4393N|mint:P38703 - - - comment:Sphingosine is [3H] radio-labelled.|kinetics:Apparent Km = 3uM for C26 FA-CoA, and 30uM for C20 FA-CoA|kinetic_conditions:pH 6.8, Temp=24C.|figure legend:Fig. 2|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2009/06/19 2014/10/16 rogid:gqRCCAHQcrRAHNO+R2LkQOzJ0mg559292 rogid:KCAjx/gXmy0oGd3aKgasDuFHJzw559292 intact-crc:E0FF44DD60371CA9 false flag tag:n-n - - - psi-mi:"MI:0093"(protein sequence identification) psi-mi:"MI:0093"(protein sequence identification) uniprotkb:P28496 uniprotkb:Q03579 intact:EBI-26585|uniprotkb:D6VXS8|ensemblfungi:YKL008C intact:EBI-27640|uniprotkb:D6W0C5|ensemblfungi:YMR298W psi-mi:lac1_yeast(display_long)|uniprotkb:LAC1(gene name)|psi-mi:LAC1(display_short)|uniprotkb:DGT1(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAC1(gene name synonym)|uniprotkb:YKL156(orf name)|uniprotkb:YKL008C(locus name) psi-mi:lip1_yeast(display_long)|uniprotkb:YMR298W(locus name)|uniprotkb:LIP1(gene name)|psi-mi:LIP1(display_short)|uniprotkb:LAG1/LAC1-interacting protein 1(gene name synonym) psi-mi:"MI:0415"(enzymatic study) Vallee et al. (2005) pubmed:15692566|imex:IM-19081 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0414"(enzymatic reaction) psi-mi:"MI:0469"(IntAct) intact:EBI-2355064|imex:IM-19081-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) sgd:S000001491|refseq:NP_012917.3|mint:P28496|dip:DIP-4348N|ensemblfungi:YKL008C(gene)|ensemblfungi:YKL008C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0034399"(nuclear periphery)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661 refseq:NP_014027.1|sgd:S000004913|dip:DIP-4392N|ensemblfungi:YMR298W(gene)|ensemblfungi:YMR298W_mRNA(transcript)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0061576"(acyl-CoA ceramide synthase complex) - - - comment:Sphingosine is [3H] radio-labelled.|kinetics:Apparent Km = 3uM for C26 FA-CoA, and 30uM for C20 FA-CoA|kinetic_conditions:pH 6.8, Temp=24C.|figure legend:Fig. 2|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2009/06/19 2014/10/16 rogid:gqRCCAHQcrRAHNO+R2LkQOzJ0mg559292 rogid:9Z3aO01R1mik+xM40fm/dy+A/ec559292 intact-crc:E0FF44DD60371CA9 false flag tag:n-n - - - psi-mi:"MI:0093"(protein sequence identification) psi-mi:"MI:0093"(protein sequence identification) chebi:"CHEBI:33184" uniprotkb:P28496 intact:EBI-2355059 intact:EBI-26585|uniprotkb:D6VXS8|ensemblfungi:YKL008C psi-mi:long-chain acyl-coas(display_short)|psi-mi:"CHEBI:33184"(display_long) psi-mi:lac1_yeast(display_long)|uniprotkb:LAC1(gene name)|psi-mi:LAC1(display_short)|uniprotkb:DGT1(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAC1(gene name synonym)|uniprotkb:YKL156(orf name)|uniprotkb:YKL008C(locus name) psi-mi:"MI:0415"(enzymatic study) Vallee et al. (2005) pubmed:15692566|imex:IM-19081 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0414"(enzymatic reaction) psi-mi:"MI:0469"(IntAct) intact:EBI-2355064|imex:IM-19081-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0502"(enzyme target) psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0328"(small molecule) psi-mi:"MI:0326"(protein) - sgd:S000001491|refseq:NP_012917.3|mint:P28496|dip:DIP-4348N|ensemblfungi:YKL008C(gene)|ensemblfungi:YKL008C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0034399"(nuclear periphery)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661 - - - comment:Sphingosine is [3H] radio-labelled.|kinetics:Apparent Km = 3uM for C26 FA-CoA, and 30uM for C20 FA-CoA|kinetic_conditions:pH 6.8, Temp=24C.|figure legend:Fig. 2|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2009/06/19 2014/10/16 - rogid:gqRCCAHQcrRAHNO+R2LkQOzJ0mg559292 intact-crc:E0FF44DD60371CA9 false - flag tag:n-n - - psi-mi:"MI:0093"(protein sequence identification) psi-mi:"MI:0093"(protein sequence identification) chebi:"CHEBI:16566" uniprotkb:P28496 intact:EBI-2355061 intact:EBI-26585|uniprotkb:D6VXS8|ensemblfungi:YKL008C psi-mi:sphinganine(display_short)|psi-mi:"CHEBI:16566"(display_long) psi-mi:lac1_yeast(display_long)|uniprotkb:LAC1(gene name)|psi-mi:LAC1(display_short)|uniprotkb:DGT1(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAC1(gene name synonym)|uniprotkb:YKL156(orf name)|uniprotkb:YKL008C(locus name) psi-mi:"MI:0415"(enzymatic study) Vallee et al. (2005) pubmed:15692566|imex:IM-19081 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0414"(enzymatic reaction) psi-mi:"MI:0469"(IntAct) intact:EBI-2355064|imex:IM-19081-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0502"(enzyme target) psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0328"(small molecule) psi-mi:"MI:0326"(protein) - sgd:S000001491|refseq:NP_012917.3|mint:P28496|dip:DIP-4348N|ensemblfungi:YKL008C(gene)|ensemblfungi:YKL008C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0034399"(nuclear periphery)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661 - - - comment:Sphingosine is [3H] radio-labelled.|kinetics:Apparent Km = 3uM for C26 FA-CoA, and 30uM for C20 FA-CoA|kinetic_conditions:pH 6.8, Temp=24C.|figure legend:Fig. 2|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2009/06/19 2014/10/16 - rogid:gqRCCAHQcrRAHNO+R2LkQOzJ0mg559292 intact-crc:E0FF44DD60371CA9 false - flag tag:n-n - - psi-mi:"MI:0093"(protein sequence identification) psi-mi:"MI:0093"(protein sequence identification) uniprotkb:P28496 uniprotkb:P38703 intact:EBI-26585|uniprotkb:D6VXS8|ensemblfungi:YKL008C intact:EBI-10035|uniprotkb:Q6LD26|uniprotkb:D3DKR1|ensemblfungi:YHL003C psi-mi:lac1_yeast(display_long)|uniprotkb:LAC1(gene name)|psi-mi:LAC1(display_short)|uniprotkb:DGT1(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAC1(gene name synonym)|uniprotkb:YKL156(orf name)|uniprotkb:YKL008C(locus name) psi-mi:lag1_yeast(display_long)|uniprotkb:LAG1(gene name)|psi-mi:LAG1(display_short)|uniprotkb:Longevity assurance factor 1(gene name synonym)|uniprotkb:Longevity assurance protein 1(gene name synonym)|uniprotkb:Longevity assurance gene 1 protein(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAG1(gene name synonym)|uniprotkb:YHL003C(locus name) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Vallee et al. (2005) pubmed:15692566|imex:IM-19081 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-2355081|imex:IM-19081-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) sgd:S000001491|refseq:NP_012917.3|mint:P28496|dip:DIP-4348N|ensemblfungi:YKL008C(gene)|ensemblfungi:YKL008C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0034399"(nuclear periphery)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661 refseq:NP_011860.1|sgd:S000000995|ensemblfungi:YHL003C(gene)|ensemblfungi:YHL003C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661|dip:DIP-4393N|mint:P38703 - - - figure legend:Fig. 3A|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/06/19 2014/10/16 rogid:gqRCCAHQcrRAHNO+R2LkQOzJ0mg559292 rogid:KCAjx/gXmy0oGd3aKgasDuFHJzw559292 intact-crc:CF6DEF1B3F484A14|rigid:60/bzojiAc+LQDVR2KsoJTfVmxU false flag tag:n-n - - - psi-mi:"MI:0093"(protein sequence identification) psi-mi:"MI:0093"(protein sequence identification) uniprotkb:P28496 uniprotkb:Q03579 intact:EBI-26585|uniprotkb:D6VXS8|ensemblfungi:YKL008C intact:EBI-27640|uniprotkb:D6W0C5|ensemblfungi:YMR298W psi-mi:lac1_yeast(display_long)|uniprotkb:LAC1(gene name)|psi-mi:LAC1(display_short)|uniprotkb:DGT1(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAC1(gene name synonym)|uniprotkb:YKL156(orf name)|uniprotkb:YKL008C(locus name) psi-mi:lip1_yeast(display_long)|uniprotkb:YMR298W(locus name)|uniprotkb:LIP1(gene name)|psi-mi:LIP1(display_short)|uniprotkb:LAG1/LAC1-interacting protein 1(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Vallee et al. (2005) pubmed:15692566|imex:IM-19081 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-2355081|imex:IM-19081-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) sgd:S000001491|refseq:NP_012917.3|mint:P28496|dip:DIP-4348N|ensemblfungi:YKL008C(gene)|ensemblfungi:YKL008C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0034399"(nuclear periphery)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661 refseq:NP_014027.1|sgd:S000004913|dip:DIP-4392N|ensemblfungi:YMR298W(gene)|ensemblfungi:YMR298W_mRNA(transcript)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0061576"(acyl-CoA ceramide synthase complex) - - - figure legend:Fig. 3A|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/06/19 2014/10/16 rogid:gqRCCAHQcrRAHNO+R2LkQOzJ0mg559292 rogid:9Z3aO01R1mik+xM40fm/dy+A/ec559292 intact-crc:CF6DEF1B3F484A14|rigid:60/bzojiAc+LQDVR2KsoJTfVmxU false flag tag:n-n - - - psi-mi:"MI:0093"(protein sequence identification) psi-mi:"MI:0093"(protein sequence identification) uniprotkb:P38703 uniprotkb:P28496 intact:EBI-10035|uniprotkb:Q6LD26|uniprotkb:D3DKR1|ensemblfungi:YHL003C intact:EBI-26585|uniprotkb:D6VXS8|ensemblfungi:YKL008C psi-mi:lag1_yeast(display_long)|uniprotkb:LAG1(gene name)|psi-mi:LAG1(display_short)|uniprotkb:Longevity assurance factor 1(gene name synonym)|uniprotkb:Longevity assurance protein 1(gene name synonym)|uniprotkb:Longevity assurance gene 1 protein(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAG1(gene name synonym)|uniprotkb:YHL003C(locus name) psi-mi:lac1_yeast(display_long)|uniprotkb:LAC1(gene name)|psi-mi:LAC1(display_short)|uniprotkb:DGT1(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAC1(gene name synonym)|uniprotkb:YKL156(orf name)|uniprotkb:YKL008C(locus name) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Vallee et al. (2005) pubmed:15692566|imex:IM-19081 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2355099|imex:IM-19081-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_011860.1|sgd:S000000995|ensemblfungi:YHL003C(gene)|ensemblfungi:YHL003C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661|dip:DIP-4393N|mint:P38703 sgd:S000001491|refseq:NP_012917.3|mint:P28496|dip:DIP-4348N|ensemblfungi:YKL008C(gene)|ensemblfungi:YKL008C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0034399"(nuclear periphery)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661 - - - figure legend:Fig. 3B|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/06/19 2014/10/16 rogid:KCAjx/gXmy0oGd3aKgasDuFHJzw559292 rogid:gqRCCAHQcrRAHNO+R2LkQOzJ0mg559292 intact-crc:F9D92B8EE6FD97EF|rigid:oUy/IHPQVgIYkKJ+L/AwQ58OfU0 false myc tag:n-n flag tag:n-n - - psi-mi:"MI:0705"(anti tag western blot) psi-mi:"MI:0705"(anti tag western blot) uniprotkb:Q03579 uniprotkb:P28496 intact:EBI-27640|uniprotkb:D6W0C5|ensemblfungi:YMR298W intact:EBI-26585|uniprotkb:D6VXS8|ensemblfungi:YKL008C psi-mi:lip1_yeast(display_long)|uniprotkb:YMR298W(locus name)|uniprotkb:LIP1(gene name)|psi-mi:LIP1(display_short)|uniprotkb:LAG1/LAC1-interacting protein 1(gene name synonym) psi-mi:lac1_yeast(display_long)|uniprotkb:LAC1(gene name)|psi-mi:LAC1(display_short)|uniprotkb:DGT1(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAC1(gene name synonym)|uniprotkb:YKL156(orf name)|uniprotkb:YKL008C(locus name) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Vallee et al. (2005) pubmed:15692566|imex:IM-19081 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2355108|imex:IM-19081-4 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_014027.1|sgd:S000004913|dip:DIP-4392N|ensemblfungi:YMR298W(gene)|ensemblfungi:YMR298W_mRNA(transcript)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0061576"(acyl-CoA ceramide synthase complex) sgd:S000001491|refseq:NP_012917.3|mint:P28496|dip:DIP-4348N|ensemblfungi:YKL008C(gene)|ensemblfungi:YKL008C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0034399"(nuclear periphery)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661 - - - figure legend:Fig. 3B|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/06/19 2014/10/16 rogid:9Z3aO01R1mik+xM40fm/dy+A/ec559292 rogid:gqRCCAHQcrRAHNO+R2LkQOzJ0mg559292 intact-crc:BF76A94C3B21E8EE|rigid:G+CNOfGxkR4WLSUBKADbgCpbKgA false myc tag:n-n flag tag:n-n - - psi-mi:"MI:0705"(anti tag western blot) psi-mi:"MI:0705"(anti tag western blot) uniprotkb:Q03579 uniprotkb:P38703 intact:EBI-27640|uniprotkb:D6W0C5|ensemblfungi:YMR298W intact:EBI-10035|uniprotkb:Q6LD26|uniprotkb:D3DKR1|ensemblfungi:YHL003C psi-mi:lip1_yeast(display_long)|uniprotkb:YMR298W(locus name)|uniprotkb:LIP1(gene name)|psi-mi:LIP1(display_short)|uniprotkb:LAG1/LAC1-interacting protein 1(gene name synonym) psi-mi:lag1_yeast(display_long)|uniprotkb:LAG1(gene name)|psi-mi:LAG1(display_short)|uniprotkb:Longevity assurance factor 1(gene name synonym)|uniprotkb:Longevity assurance protein 1(gene name synonym)|uniprotkb:Longevity assurance gene 1 protein(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAG1(gene name synonym)|uniprotkb:YHL003C(locus name) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Vallee et al. (2005) pubmed:15692566|imex:IM-19081 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2355120|imex:IM-19081-5 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_014027.1|sgd:S000004913|dip:DIP-4392N|ensemblfungi:YMR298W(gene)|ensemblfungi:YMR298W_mRNA(transcript)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0061576"(acyl-CoA ceramide synthase complex) refseq:NP_011860.1|sgd:S000000995|ensemblfungi:YHL003C(gene)|ensemblfungi:YHL003C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661|dip:DIP-4393N|mint:P38703 - - - figure legend:Fig. 3B|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/06/19 2014/10/16 rogid:9Z3aO01R1mik+xM40fm/dy+A/ec559292 rogid:KCAjx/gXmy0oGd3aKgasDuFHJzw559292 intact-crc:24EEEECB3F20CB30|rigid:KG6wjVoMbf9+3GsX2vhdBl+5M9w false flag tag:n-n myc tag:n-n - - psi-mi:"MI:0705"(anti tag western blot) psi-mi:"MI:0705"(anti tag western blot) uniprotkb:Q03579 uniprotkb:P28496 intact:EBI-27640|uniprotkb:D6W0C5|ensemblfungi:YMR298W intact:EBI-26585|uniprotkb:D6VXS8|ensemblfungi:YKL008C psi-mi:lip1_yeast(display_long)|uniprotkb:YMR298W(locus name)|uniprotkb:LIP1(gene name)|psi-mi:LIP1(display_short)|uniprotkb:LAG1/LAC1-interacting protein 1(gene name synonym) psi-mi:lac1_yeast(display_long)|uniprotkb:LAC1(gene name)|psi-mi:LAC1(display_short)|uniprotkb:DGT1(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAC1(gene name synonym)|uniprotkb:YKL156(orf name)|uniprotkb:YKL008C(locus name) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Vallee et al. (2005) pubmed:15692566|imex:IM-19081 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-2355129|imex:IM-19081-6 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_014027.1|sgd:S000004913|dip:DIP-4392N|ensemblfungi:YMR298W(gene)|ensemblfungi:YMR298W_mRNA(transcript)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0061576"(acyl-CoA ceramide synthase complex) sgd:S000001491|refseq:NP_012917.3|mint:P28496|dip:DIP-4348N|ensemblfungi:YKL008C(gene)|ensemblfungi:YKL008C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0034399"(nuclear periphery)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661 - - - figure legend:Fig. 3C|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/06/19 2014/10/16 rogid:9Z3aO01R1mik+xM40fm/dy+A/ec559292 rogid:gqRCCAHQcrRAHNO+R2LkQOzJ0mg559292 intact-crc:B22122DD7BBF4010|rigid:60/bzojiAc+LQDVR2KsoJTfVmxU false flag tag:n-n ha tag:n-n - - psi-mi:"MI:0705"(anti tag western blot) psi-mi:"MI:0705"(anti tag western blot) uniprotkb:Q03579 uniprotkb:P38703 intact:EBI-27640|uniprotkb:D6W0C5|ensemblfungi:YMR298W intact:EBI-10035|uniprotkb:Q6LD26|uniprotkb:D3DKR1|ensemblfungi:YHL003C psi-mi:lip1_yeast(display_long)|uniprotkb:YMR298W(locus name)|uniprotkb:LIP1(gene name)|psi-mi:LIP1(display_short)|uniprotkb:LAG1/LAC1-interacting protein 1(gene name synonym) psi-mi:lag1_yeast(display_long)|uniprotkb:LAG1(gene name)|psi-mi:LAG1(display_short)|uniprotkb:Longevity assurance factor 1(gene name synonym)|uniprotkb:Longevity assurance protein 1(gene name synonym)|uniprotkb:Longevity assurance gene 1 protein(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAG1(gene name synonym)|uniprotkb:YHL003C(locus name) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Vallee et al. (2005) pubmed:15692566|imex:IM-19081 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-2355129|imex:IM-19081-6 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_014027.1|sgd:S000004913|dip:DIP-4392N|ensemblfungi:YMR298W(gene)|ensemblfungi:YMR298W_mRNA(transcript)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0061576"(acyl-CoA ceramide synthase complex) refseq:NP_011860.1|sgd:S000000995|ensemblfungi:YHL003C(gene)|ensemblfungi:YHL003C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661|dip:DIP-4393N|mint:P38703 - - - figure legend:Fig. 3C|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/06/19 2014/10/16 rogid:9Z3aO01R1mik+xM40fm/dy+A/ec559292 rogid:KCAjx/gXmy0oGd3aKgasDuFHJzw559292 intact-crc:B22122DD7BBF4010|rigid:60/bzojiAc+LQDVR2KsoJTfVmxU false flag tag:n-n myc tag:n-n - - psi-mi:"MI:0705"(anti tag western blot) psi-mi:"MI:0705"(anti tag western blot) uniprotkb:P28496 uniprotkb:P38703 intact:EBI-26585|uniprotkb:D6VXS8|ensemblfungi:YKL008C intact:EBI-10035|uniprotkb:Q6LD26|uniprotkb:D3DKR1|ensemblfungi:YHL003C psi-mi:lac1_yeast(display_long)|uniprotkb:LAC1(gene name)|psi-mi:LAC1(display_short)|uniprotkb:DGT1(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAC1(gene name synonym)|uniprotkb:YKL156(orf name)|uniprotkb:YKL008C(locus name) psi-mi:lag1_yeast(display_long)|uniprotkb:LAG1(gene name)|psi-mi:LAG1(display_short)|uniprotkb:Longevity assurance factor 1(gene name synonym)|uniprotkb:Longevity assurance protein 1(gene name synonym)|uniprotkb:Longevity assurance gene 1 protein(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAG1(gene name synonym)|uniprotkb:YHL003C(locus name) psi-mi:"MI:0028"(cosedimentation in solution) Vallee et al. (2005) pubmed:15692566|imex:IM-19081 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2355147|imex:IM-19081-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) sgd:S000001491|refseq:NP_012917.3|mint:P28496|dip:DIP-4348N|ensemblfungi:YKL008C(gene)|ensemblfungi:YKL008C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0034399"(nuclear periphery)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661 refseq:NP_011860.1|sgd:S000000995|ensemblfungi:YHL003C(gene)|ensemblfungi:YHL003C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661|dip:DIP-4393N|mint:P38703 - - - figure legend:Fig. 5|complex-properties:complex mass = approximately 250�260 kDa.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/06/19 2014/10/16 rogid:gqRCCAHQcrRAHNO+R2LkQOzJ0mg559292 rogid:KCAjx/gXmy0oGd3aKgasDuFHJzw559292 intact-crc:6D4CD494DF78159C|rigid:60/bzojiAc+LQDVR2KsoJTfVmxU false flag tag:n-n - 1 1 psi-mi:"MI:0817"(molecular weight estimation by silver staining) psi-mi:"MI:0817"(molecular weight estimation by silver staining) uniprotkb:P28496 uniprotkb:Q03579 intact:EBI-26585|uniprotkb:D6VXS8|ensemblfungi:YKL008C intact:EBI-27640|uniprotkb:D6W0C5|ensemblfungi:YMR298W psi-mi:lac1_yeast(display_long)|uniprotkb:LAC1(gene name)|psi-mi:LAC1(display_short)|uniprotkb:DGT1(gene name synonym)|uniprotkb:Very-long-chain ceramide synthase LAC1(gene name synonym)|uniprotkb:YKL156(orf name)|uniprotkb:YKL008C(locus name) psi-mi:lip1_yeast(display_long)|uniprotkb:YMR298W(locus name)|uniprotkb:LIP1(gene name)|psi-mi:LIP1(display_short)|uniprotkb:LAG1/LAC1-interacting protein 1(gene name synonym) psi-mi:"MI:0028"(cosedimentation in solution) Vallee et al. (2005) pubmed:15692566|imex:IM-19081 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-2355147|imex:IM-19081-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) sgd:S000001491|refseq:NP_012917.3|mint:P28496|dip:DIP-4348N|ensemblfungi:YKL008C(gene)|ensemblfungi:YKL008C_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016410"(N-acyltransferase activity)|go:"GO:0034399"(nuclear periphery)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0050291"(sphingosine N-acyltransferase activity)|go:"GO:0061576"(acyl-CoA ceramide synthase complex)|interpro:IPR006634(TRAM, LAG1 and CLN8 homology)|interpro:IPR013599(TRAM1-like protein)|interpro:IPR016439(Longevity assurance, LAG1/LAC1)|reactome:R-SCE-1660661 refseq:NP_014027.1|sgd:S000004913|dip:DIP-4392N|ensemblfungi:YMR298W(gene)|ensemblfungi:YMR298W_mRNA(transcript)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0046513"(ceramide biosynthetic process)|go:"GO:0061576"(acyl-CoA ceramide synthase complex) - - - figure legend:Fig. 5|complex-properties:complex mass = approximately 250�260 kDa.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2009/06/19 2014/10/16 rogid:gqRCCAHQcrRAHNO+R2LkQOzJ0mg559292 rogid:9Z3aO01R1mik+xM40fm/dy+A/ec559292 intact-crc:6D4CD494DF78159C|rigid:60/bzojiAc+LQDVR2KsoJTfVmxU false flag tag:n-n - 1 2 psi-mi:"MI:0817"(molecular weight estimation by silver staining) psi-mi:"MI:0817"(molecular weight estimation by silver staining)