#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P23301 uniprotkb:P38791 intact:EBI-9033|ensemblfungi:YEL034W|uniprotkb:D3DLL5 intact:EBI-5871|uniprotkb:D3DL17|ensemblfungi:YHR068W psi-mi:if5a1_yeast(display_long)|uniprotkb:Hypusine-containing protein HP2(gene name synonym)|uniprotkb:TIF51A(gene name synonym)|uniprotkb:SYGP-ORF21(orf name)|uniprotkb:YEL034W(locus name)|uniprotkb:HYP2(gene name)|psi-mi:HYP2(display_short)|uniprotkb:eIF-4D(gene name synonym) psi-mi:dhys_yeast(display_long)|uniprotkb:YHR068W(locus name)|uniprotkb:DYS1(gene name)|psi-mi:DYS1(display_short) psi-mi:"MI:0018"(two hybrid) Thompson et al. (2003) pubmed:14675757|imex:IM-15862|mint:MINT-7026179|doi:"10.1016/S0014-5793(03)01305-X" taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0471"(MINT) intact:EBI-7072330|mint:MINT-7026191|imex:IM-15862-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR014722(Translation protein SH3-like, subgroup)|interpro:IPR019769|interpro:IPR020189|rcsb pdb:3ER0|rcsb pdb:5DAT|rcsb pdb:5DC3|rcsb pdb:5DGE|rcsb pdb:5DGF|rcsb pdb:5GAK|rcsb pdb:5MC6|rcsb pdb:6Q84|rcsb pdb:6TNU|rcsb pdb:7NRC|reactome:R-SCE-204626|ensemblfungi:YEL034W(gene)|ensemblfungi:YEL034W_mRNA(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0003746"(translation elongation factor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0006452"(translational frameshifting)|go:"GO:0043022"(ribosome binding)|go:"GO:0045901"(positive regulation of translational elongation)|go:"GO:0045905"(positive regulation of translational termination)|go:"GO:0045948"(positive regulation of translational initiation)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:1903272"(positive regulation of cytoplasmic translational elongation through polyproline stretches)|interpro:IPR001884("Eukaryotic initiation factor 5A hypusine (eIF-5A)")|interpro:IPR008991(Translation protein SH3-like)|refseq:NP_010880.3|sgd:S000000760|mint:P23301|dip:DIP-4230N|mint:MINT-7026193(identity) refseq:NP_011935.1|sgd:S000001110|dip:DIP-2788N|mint:P38791|ensemblfungi:YHR068W(gene)|ensemblfungi:YHR068W_mRNA(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0008612"(peptidyl-lysine modification to peptidyl-hypusine)|go:"GO:0034038"(deoxyhypusine synthase activity)|interpro:IPR002773(Deoxyhypusine synthase)|interpro:IPR029035|interpro:IPR036982|reactome:R-SCE-204626|mint:MINT-7026194(identity) - crc64:738F29CCC7820C47 - comment:Thus, although the target site is located in the N-terminal domain, the modifying enzyme interacts with both domains of eIF5A. The truncation of eIF5A residues 1–42 or 1–63 weakens the interaction with Dys1 probably due to a conformational change in eIF5A structure, suggesting that the correct folding of the N-terminal domain of eIF5A is essential for efficient interaction with Dys1.|comment:mint|figure legend:F1 F3C|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) - 2009/04/08 2016/10/10 rogid:B9tQQwPUHYgFdA1V67iSN7G/PtY559292 rogid:nnqQ22LNFXyfdSqHeg3mu/iXPuI559292 intact-crc:B089012F5AB9B867|rigid:01dNZ1e+egCCk8lW9WN2ICfmp+E false tag:?-?(MINT-7026196) binding-associated region:29-378(MINT-7026203)|tag:?-?(MINT-7026200) - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P23301 uniprotkb:P47120 intact:EBI-9033|ensemblfungi:YEL034W|uniprotkb:D3DLL5 intact:EBI-25526|uniprotkb:D6VWP1|ensemblfungi:YJR070C psi-mi:if5a1_yeast(display_long)|uniprotkb:Hypusine-containing protein HP2(gene name synonym)|uniprotkb:TIF51A(gene name synonym)|uniprotkb:SYGP-ORF21(orf name)|uniprotkb:YEL034W(locus name)|uniprotkb:HYP2(gene name)|psi-mi:HYP2(display_short)|uniprotkb:eIF-4D(gene name synonym) psi-mi:dohh_yeast(display_long)|uniprotkb:YJR070C(locus name)|uniprotkb:J1814(orf name)|uniprotkb:Deoxyhypusine monooxygenase(gene name synonym)|uniprotkb:Ligand of eIF5A protein 1(gene name synonym)|uniprotkb:Deoxyhypusine dioxygenase(gene name synonym)|uniprotkb:LIA1(gene name)|psi-mi:LIA1(display_short)|uniprotkb:MMD1(gene name synonym) psi-mi:"MI:0018"(two hybrid) Thompson et al. (2003) pubmed:14675757|imex:IM-15862|mint:MINT-7026179|doi:"10.1016/S0014-5793(03)01305-X" taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0471"(MINT) intact:EBI-7072356|mint:MINT-7026212|imex:IM-15862-2 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR014722(Translation protein SH3-like, subgroup)|interpro:IPR019769|interpro:IPR020189|rcsb pdb:3ER0|rcsb pdb:5DAT|rcsb pdb:5DC3|rcsb pdb:5DGE|rcsb pdb:5DGF|rcsb pdb:5GAK|rcsb pdb:5MC6|rcsb pdb:6Q84|rcsb pdb:6TNU|rcsb pdb:7NRC|reactome:R-SCE-204626|ensemblfungi:YEL034W(gene)|ensemblfungi:YEL034W_mRNA(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0003746"(translation elongation factor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0006452"(translational frameshifting)|go:"GO:0043022"(ribosome binding)|go:"GO:0045901"(positive regulation of translational elongation)|go:"GO:0045905"(positive regulation of translational termination)|go:"GO:0045948"(positive regulation of translational initiation)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:1903272"(positive regulation of cytoplasmic translational elongation through polyproline stretches)|interpro:IPR001884("Eukaryotic initiation factor 5A hypusine (eIF-5A)")|interpro:IPR008991(Translation protein SH3-like)|refseq:NP_010880.3|sgd:S000000760|mint:P23301|dip:DIP-4230N|mint:MINT-7026220(identity) refseq:NP_012604.1|sgd:S000003831|mint:P47120|dip:DIP-2829N|ensemblfungi:YJR070C(gene)|ensemblfungi:YJR070C_mRNA(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0008612"(peptidyl-lysine modification to peptidyl-hypusine)|go:"GO:0016491"(oxidoreductase activity)|go:"GO:0019135"(deoxyhypusine monooxygenase activity)|go:"GO:0046872"(metal ion binding)|interpro:IPR004155(PBS lyase HEAT-like repeat)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR027517|reactome:R-SCE-204626|mint:MINT-7026214(identity) - crc64:738F29CCC7820C47 crc64:AB36A73B40466CD5 comment:mint|figure legend:F1 F3C|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) - 2009/04/08 2014/10/16 rogid:B9tQQwPUHYgFdA1V67iSN7G/PtY559292 rogid:ZqdyFodW8hqdOsOgIizarhUDHKY559292 intact-crc:212CA1C48954B215|rigid:S2IdxnwpR/KaU2ynigXvHckABrc false binding-associated region:91-157(MINT-7026229)|tag:?-?(MINT-7026223) tag:?-?(MINT-7026217) - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P23301 uniprotkb:P38791 intact:EBI-9033|ensemblfungi:YEL034W|uniprotkb:D3DLL5 intact:EBI-5871|uniprotkb:D3DL17|ensemblfungi:YHR068W psi-mi:if5a1_yeast(display_long)|uniprotkb:Hypusine-containing protein HP2(gene name synonym)|uniprotkb:TIF51A(gene name synonym)|uniprotkb:SYGP-ORF21(orf name)|uniprotkb:YEL034W(locus name)|uniprotkb:HYP2(gene name)|psi-mi:HYP2(display_short)|uniprotkb:eIF-4D(gene name synonym) psi-mi:dhys_yeast(display_long)|uniprotkb:YHR068W(locus name)|uniprotkb:DYS1(gene name)|psi-mi:DYS1(display_short) psi-mi:"MI:0096"(pull down) Thompson et al. (2003) pubmed:14675757|imex:IM-15862|mint:MINT-7026179|doi:"10.1016/S0014-5793(03)01305-X" taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0471"(MINT) intact:EBI-7072402|mint:MINT-7026233|imex:IM-15862-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR014722(Translation protein SH3-like, subgroup)|interpro:IPR019769|interpro:IPR020189|rcsb pdb:3ER0|rcsb pdb:5DAT|rcsb pdb:5DC3|rcsb pdb:5DGE|rcsb pdb:5DGF|rcsb pdb:5GAK|rcsb pdb:5MC6|rcsb pdb:6Q84|rcsb pdb:6TNU|rcsb pdb:7NRC|reactome:R-SCE-204626|ensemblfungi:YEL034W(gene)|ensemblfungi:YEL034W_mRNA(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0003746"(translation elongation factor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0006452"(translational frameshifting)|go:"GO:0043022"(ribosome binding)|go:"GO:0045901"(positive regulation of translational elongation)|go:"GO:0045905"(positive regulation of translational termination)|go:"GO:0045948"(positive regulation of translational initiation)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:1903272"(positive regulation of cytoplasmic translational elongation through polyproline stretches)|interpro:IPR001884("Eukaryotic initiation factor 5A hypusine (eIF-5A)")|interpro:IPR008991(Translation protein SH3-like)|refseq:NP_010880.3|sgd:S000000760|mint:P23301|dip:DIP-4230N|mint:MINT-7026241(identity) refseq:NP_011935.1|sgd:S000001110|dip:DIP-2788N|mint:P38791|ensemblfungi:YHR068W(gene)|ensemblfungi:YHR068W_mRNA(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0008612"(peptidyl-lysine modification to peptidyl-hypusine)|go:"GO:0034038"(deoxyhypusine synthase activity)|interpro:IPR002773(Deoxyhypusine synthase)|interpro:IPR029035|interpro:IPR036982|reactome:R-SCE-204626|mint:MINT-7026235(identity) - crc64:738F29CCC7820C47 - figure legend:F2A|comment:mint|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) - 2009/04/08 2014/10/16 rogid:B9tQQwPUHYgFdA1V67iSN7G/PtY559292 rogid:nnqQ22LNFXyfdSqHeg3mu/iXPuI559292 intact-crc:6733DA10CEDC132B|rigid:01dNZ1e+egCCk8lW9WN2ICfmp+E false glutathione s tranferase tag:?-?(MINT-7026244) - - - psi-mi:"MI:0705"(anti tag western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P23301 uniprotkb:P47120 intact:EBI-9033|ensemblfungi:YEL034W|uniprotkb:D3DLL5 intact:EBI-25526|uniprotkb:D6VWP1|ensemblfungi:YJR070C psi-mi:if5a1_yeast(display_long)|uniprotkb:Hypusine-containing protein HP2(gene name synonym)|uniprotkb:TIF51A(gene name synonym)|uniprotkb:SYGP-ORF21(orf name)|uniprotkb:YEL034W(locus name)|uniprotkb:HYP2(gene name)|psi-mi:HYP2(display_short)|uniprotkb:eIF-4D(gene name synonym) psi-mi:dohh_yeast(display_long)|uniprotkb:YJR070C(locus name)|uniprotkb:J1814(orf name)|uniprotkb:Deoxyhypusine monooxygenase(gene name synonym)|uniprotkb:Ligand of eIF5A protein 1(gene name synonym)|uniprotkb:Deoxyhypusine dioxygenase(gene name synonym)|uniprotkb:LIA1(gene name)|psi-mi:LIA1(display_short)|uniprotkb:MMD1(gene name synonym) psi-mi:"MI:0096"(pull down) Thompson et al. (2003) pubmed:14675757|imex:IM-15862|mint:MINT-7026179|doi:"10.1016/S0014-5793(03)01305-X" taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0471"(MINT) intact:EBI-7072430|mint:MINT-7026266|imex:IM-15862-5 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR014722(Translation protein SH3-like, subgroup)|interpro:IPR019769|interpro:IPR020189|rcsb pdb:3ER0|rcsb pdb:5DAT|rcsb pdb:5DC3|rcsb pdb:5DGE|rcsb pdb:5DGF|rcsb pdb:5GAK|rcsb pdb:5MC6|rcsb pdb:6Q84|rcsb pdb:6TNU|rcsb pdb:7NRC|reactome:R-SCE-204626|ensemblfungi:YEL034W(gene)|ensemblfungi:YEL034W_mRNA(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0003746"(translation elongation factor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0006452"(translational frameshifting)|go:"GO:0043022"(ribosome binding)|go:"GO:0045901"(positive regulation of translational elongation)|go:"GO:0045905"(positive regulation of translational termination)|go:"GO:0045948"(positive regulation of translational initiation)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:1903272"(positive regulation of cytoplasmic translational elongation through polyproline stretches)|interpro:IPR001884("Eukaryotic initiation factor 5A hypusine (eIF-5A)")|interpro:IPR008991(Translation protein SH3-like)|refseq:NP_010880.3|sgd:S000000760|mint:P23301|dip:DIP-4230N|mint:MINT-7026272(identity) refseq:NP_012604.1|sgd:S000003831|mint:P47120|dip:DIP-2829N|ensemblfungi:YJR070C(gene)|ensemblfungi:YJR070C_mRNA(transcript)|go:"GO:0000226"(microtubule cytoskeleton organization)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0008612"(peptidyl-lysine modification to peptidyl-hypusine)|go:"GO:0016491"(oxidoreductase activity)|go:"GO:0019135"(deoxyhypusine monooxygenase activity)|go:"GO:0046872"(metal ion binding)|interpro:IPR004155(PBS lyase HEAT-like repeat)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR027517|reactome:R-SCE-204626|mint:MINT-7026268(identity) - crc64:738F29CCC7820C47 crc64:AB36A73B40466CD5 figure legend:F2C|comment:mint|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) - 2009/04/08 2014/10/16 rogid:B9tQQwPUHYgFdA1V67iSN7G/PtY559292 rogid:ZqdyFodW8hqdOsOgIizarhUDHKY559292 intact-crc:C64F767717D9509C|rigid:S2IdxnwpR/KaU2ynigXvHckABrc false - glutathione s tranferase tag:?-?(MINT-7026269) - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0705"(anti tag western blot) uniprotkb:P23301 uniprotkb:P38791 intact:EBI-9033|ensemblfungi:YEL034W|uniprotkb:D3DLL5 intact:EBI-5871|uniprotkb:D3DL17|ensemblfungi:YHR068W psi-mi:if5a1_yeast(display_long)|uniprotkb:Hypusine-containing protein HP2(gene name synonym)|uniprotkb:TIF51A(gene name synonym)|uniprotkb:SYGP-ORF21(orf name)|uniprotkb:YEL034W(locus name)|uniprotkb:HYP2(gene name)|psi-mi:HYP2(display_short)|uniprotkb:eIF-4D(gene name synonym) psi-mi:dhys_yeast(display_long)|uniprotkb:YHR068W(locus name)|uniprotkb:DYS1(gene name)|psi-mi:DYS1(display_short) psi-mi:"MI:0096"(pull down) Thompson et al. (2003) pubmed:14675757|imex:IM-15862|mint:MINT-7026179|doi:"10.1016/S0014-5793(03)01305-X" taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0471"(MINT) intact:EBI-7072416|mint:MINT-7026252|imex:IM-15862-4 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR014722(Translation protein SH3-like, subgroup)|interpro:IPR019769|interpro:IPR020189|rcsb pdb:3ER0|rcsb pdb:5DAT|rcsb pdb:5DC3|rcsb pdb:5DGE|rcsb pdb:5DGF|rcsb pdb:5GAK|rcsb pdb:5MC6|rcsb pdb:6Q84|rcsb pdb:6TNU|rcsb pdb:7NRC|reactome:R-SCE-204626|ensemblfungi:YEL034W(gene)|ensemblfungi:YEL034W_mRNA(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0003746"(translation elongation factor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0006452"(translational frameshifting)|go:"GO:0043022"(ribosome binding)|go:"GO:0045901"(positive regulation of translational elongation)|go:"GO:0045905"(positive regulation of translational termination)|go:"GO:0045948"(positive regulation of translational initiation)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:1903272"(positive regulation of cytoplasmic translational elongation through polyproline stretches)|interpro:IPR001884("Eukaryotic initiation factor 5A hypusine (eIF-5A)")|interpro:IPR008991(Translation protein SH3-like)|refseq:NP_010880.3|sgd:S000000760|mint:P23301|dip:DIP-4230N|mint:MINT-7026254(identity) refseq:NP_011935.1|sgd:S000001110|dip:DIP-2788N|mint:P38791|ensemblfungi:YHR068W(gene)|ensemblfungi:YHR068W_mRNA(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0008612"(peptidyl-lysine modification to peptidyl-hypusine)|go:"GO:0034038"(deoxyhypusine synthase activity)|interpro:IPR002773(Deoxyhypusine synthase)|interpro:IPR029035|interpro:IPR036982|reactome:R-SCE-204626|mint:MINT-7026258(identity) - crc64:738F29CCC7820C47 - figure legend:F2B|comment:mint|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) - 2009/04/08 2014/10/16 rogid:B9tQQwPUHYgFdA1V67iSN7G/PtY559292 rogid:nnqQ22LNFXyfdSqHeg3mu/iXPuI559292 intact-crc:AAC66C85908F08BD|rigid:01dNZ1e+egCCk8lW9WN2ICfmp+E false - glutathione s tranferase tag:?-?(MINT-7026263) - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0705"(anti tag western blot)