#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q43725	uniprotkb:Q39218	intact:EBI-1633616|uniprotkb:Q8L4A2|uniprotkb:Q9M1Z8|uniprotkb:Q9M440|ensemblplants:AT3G59760.3	intact:EBI-1633440|uniprotkb:Q42532|uniprotkb:Q43739|uniprotkb:Q43740|uniprotkb:Q7DLZ8|uniprotkb:Q7DM01|uniprotkb:Q0V7U9|uniprotkb:Q0WWV4|ensemblplants:AT3G13110.1	psi-mi:cyskm_arath(display_long)|uniprotkb:Beta-substituted Ala synthase 2;2(gene name synonym)|uniprotkb:OASC(gene name)|psi-mi:OASC(display_short)|uniprotkb:ACS 1(gene name synonym)|uniprotkb:F24G16.30(orf name)|uniprotkb:At3g59760(locus name)|uniprotkb:O-acetylserine sulfhydrylase(gene name synonym)|uniprotkb:CSase C(gene name synonym)|uniprotkb:"O-acetylserine (thiol)-lyase"(gene name synonym)|uniprotkb:OAS-TL C(gene name synonym)	psi-mi:sat3_arath(display_long)|uniprotkb:SAT3(gene name)|psi-mi:SAT3(display_short)|uniprotkb:SAT1(gene name synonym)|uniprotkb:SATA(gene name synonym)|uniprotkb:MJG19.6(orf name)|uniprotkb:At3g13110(locus name)	psi-mi:"MI:0096"(pull down)	Berkowitz et al. (2002)	pubmed:12063244|imex:IM-19026	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2361534|imex:IM-19026-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_191535.2|dip:DIP-40500N|ensemblplants:AT3G59760(gene)|ensemblplants:AT3G59760.3(transcript)|go:"GO:0004124"(cysteine synthase activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0006535"(cysteine biosynthetic process from serine)|go:"GO:0009567"(double fertilization forming a zygote and endosperm)|go:"GO:0009860"(pollen tube growth)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0042802"(identical protein binding)|interpro:IPR001216(Cysteine synthase/cystathionine beta-synthase P-phosphate-binding site)|interpro:IPR001926(Pyridoxal phosphate-dependent enzyme, beta subunit)|interpro:IPR005856(Cysteine synthase K/M)|interpro:IPR005859(Cysteine synthase A)|interpro:IPR036052|rcsb pdb:4AEC	dip:DIP-40499N|refseq:NP_187918.1|ensemblplants:AT3G13110(gene)|ensemblplants:AT3G13110.1(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006535"(cysteine biosynthetic process from serine)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009001"(serine O-acetyltransferase activity)|go:"GO:0019344"(cysteine biosynthetic process)|go:"GO:0046686"(response to cadmium ion)|interpro:IPR001451(Bacterial transferase hexapeptide repeat)|interpro:IPR005881(Serine O-acetyltransferase)|interpro:IPR010493(Serine acetyltransferase, N-terminal)|interpro:IPR011004(Trimeric LpxA-like)|interpro:IPR018357|interpro:IPR042122	-	-	-	figure legend:Figure 2|comment:The authors demonstrated that OAS-TL was specifically and completely dissociated from column-bound SAT by the reaction intermediate OAS and regained full activity as a free homodimer.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2009/07/02	2014/10/16	rogid:SET8/ZcNUeo9dXPrkEidUZa8P1w3702	rogid:QKbM3LWfEgCpoT4skpTO44Haca43702	intact-crc:8478F21C3B289569|rigid:SJxqflvm/q7Ecr7E1GC0F2QUdPc	false	-	ha tag:n-n	2	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:Q39218	uniprotkb:Q43725	intact:EBI-1633440|uniprotkb:Q42532|uniprotkb:Q43739|uniprotkb:Q43740|uniprotkb:Q7DLZ8|uniprotkb:Q7DM01|uniprotkb:Q0V7U9|uniprotkb:Q0WWV4|ensemblplants:AT3G13110.1	intact:EBI-1633616|uniprotkb:Q8L4A2|uniprotkb:Q9M1Z8|uniprotkb:Q9M440|ensemblplants:AT3G59760.3	psi-mi:sat3_arath(display_long)|uniprotkb:SAT3(gene name)|psi-mi:SAT3(display_short)|uniprotkb:SAT1(gene name synonym)|uniprotkb:SATA(gene name synonym)|uniprotkb:MJG19.6(orf name)|uniprotkb:At3g13110(locus name)	psi-mi:cyskm_arath(display_long)|uniprotkb:Beta-substituted Ala synthase 2;2(gene name synonym)|uniprotkb:OASC(gene name)|psi-mi:OASC(display_short)|uniprotkb:ACS 1(gene name synonym)|uniprotkb:F24G16.30(orf name)|uniprotkb:At3g59760(locus name)|uniprotkb:O-acetylserine sulfhydrylase(gene name synonym)|uniprotkb:CSase C(gene name synonym)|uniprotkb:"O-acetylserine (thiol)-lyase"(gene name synonym)|uniprotkb:OAS-TL C(gene name synonym)	psi-mi:"MI:0107"(surface plasmon resonance)	Berkowitz et al. (2002)	pubmed:12063244|imex:IM-19026	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-2361548|imex:IM-19026-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	dip:DIP-40499N|refseq:NP_187918.1|ensemblplants:AT3G13110(gene)|ensemblplants:AT3G13110.1(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006535"(cysteine biosynthetic process from serine)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009001"(serine O-acetyltransferase activity)|go:"GO:0019344"(cysteine biosynthetic process)|go:"GO:0046686"(response to cadmium ion)|interpro:IPR001451(Bacterial transferase hexapeptide repeat)|interpro:IPR005881(Serine O-acetyltransferase)|interpro:IPR010493(Serine acetyltransferase, N-terminal)|interpro:IPR011004(Trimeric LpxA-like)|interpro:IPR018357|interpro:IPR042122	refseq:NP_191535.2|dip:DIP-40500N|ensemblplants:AT3G59760(gene)|ensemblplants:AT3G59760.3(transcript)|go:"GO:0004124"(cysteine synthase activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0006535"(cysteine biosynthetic process from serine)|go:"GO:0009567"(double fertilization forming a zygote and endosperm)|go:"GO:0009860"(pollen tube growth)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0042802"(identical protein binding)|interpro:IPR001216(Cysteine synthase/cystathionine beta-synthase P-phosphate-binding site)|interpro:IPR001926(Pyridoxal phosphate-dependent enzyme, beta subunit)|interpro:IPR005856(Cysteine synthase K/M)|interpro:IPR005859(Cysteine synthase A)|interpro:IPR036052|rcsb pdb:4AEC	-	-	-	figure legend:Figure 4|kinetics:association rate constant ka = 5.1 x 10-4  M-1 S-1|comment:"The authors concluded that the linear association kinetics over a 10-fold concentration range of the analyte OAS-TL strongly suggests that a simple binding model is applicable to describe the interaction between SAT and OAS-TL oligomers as proposed by a model where the assumption of an A + B  AB binding model where A would represent a SAT tetramer and B an OAS-TL dimer. They noted \" Although the dissociation phase showed some deviation from the A + B  AB model in very late stages (>900 s), its kinetic analysis still provided a good approximation\". See text for more details."|kinetics:dissociation rate constant  kd = 1.3 x 10-3 S-1|kinetics:"equilibrium dissociation constant (KD)  = 25 +/- 4 x 10-9 M (n = 4)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2009/07/02	2014/10/16	rogid:QKbM3LWfEgCpoT4skpTO44Haca43702	rogid:SET8/ZcNUeo9dXPrkEidUZa8P1w3702	intact-crc:29CA1FB64F3FF2BA|rigid:SJxqflvm/q7Ecr7E1GC0F2QUdPc	false	his tag:n-n	-	4	2	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)