#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P22515	intact:EBI-19797	intact:EBI-19703|uniprotkb:D6VWZ3|ensemblfungi:YKL210W	dip:DIP-1549N|uniprotkb:P04838|uniprotkb:Q6LA96	psi-mi:uba1_yeast(display_long)|uniprotkb:UBA1(gene name)|psi-mi:UBA1(display_short)|uniprotkb:YKL210W(locus name)	psi-mi:ubiq_yeast(display_short)|psi-mi:EBI-19797(display_long)|uniprotkb:UBI1(gene name)|psi-mi:UBI1(display_short)|uniprotkb:RPL40A(gene name synonym)|uniprotkb:YIL148W(locus name)|uniprotkb:UBI2(gene name)|psi-mi:UBI2(display_short)|uniprotkb:RPL40B(gene name synonym)|uniprotkb:YKR094C(locus name)|uniprotkb:UBI3(gene name)|psi-mi:UBI3(display_short)|uniprotkb:RPS31(gene name synonym)|uniprotkb:YLR167W(locus name)|uniprotkb:L9470.14(orf name)|uniprotkb:UBI4(gene name)|psi-mi:UBI4(display_short)|uniprotkb:SCD2(gene name synonym)|uniprotkb:YLL039C(locus name)	psi-mi:"MI:0114"(x-ray crystallography)	Lee et al. (2008)	imex:IM-11908|pubmed:18662542	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-1808635|wwpdb:3CMM|imex:IM-11908-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_012712.1|sgd:S000001693|ensemblfungi:YKL210W(gene)|ensemblfungi:YKL210W_mRNA(transcript)|go:"GO:0004839"(ubiquitin activating enzyme activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0006974"(cellular response to DNA damage stimulus)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0032446"(protein modification by small protein conjugation)|interpro:IPR000011(Ubiquitin-activating enzyme, E1)|interpro:IPR000594(UBA/THIF-type NAD/FAD binding fold)|interpro:IPR018074|interpro:IPR018075|interpro:IPR018965|interpro:IPR019572|interpro:IPR032418|interpro:IPR032420|interpro:IPR033127|interpro:IPR035985|interpro:IPR038252|interpro:IPR042063|interpro:IPR042302|interpro:IPR042449|rcsb pdb:3CMM|rcsb pdb:4NNJ|rcsb pdb:5L6H|rcsb pdb:5L6I|rcsb pdb:5L6J|rcsb pdb:5TR4|rcsb pdb:6NYA|rcsb pdb:6ZQH|rcsb pdb:7K5J|reactome:R-SCE-1169408|reactome:R-SCE-8866652|reactome:R-SCE-983168|dip:DIP-4853N|mint:P22515	sgd:S000001410(RPL40A)|sgd:S000001802(RPL40B)|sgd:S000004157(RPS31)|sgd:S000003962(UBI4)|uniprotkb:P61864(see-also)|refseq:NP_012118.1|refseq:NP_013020.1|refseq:NP_013061.1|refseq:NP_013268.1|ensembl:YIL148W|ensembl:YKR094C|ensembl:YLR167W|go:"GO:0005515"(protein binding)|interpro:IPR000626(Ubiquitin)|cygd:YLR167w|go:"GO:0022625"(cytosolic large ribosomal subunit)|go:"GO:0005739"(mitochondrion)|go:"GO:0003735"(structural constituent of ribosome)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0042254"(ribosome biogenesis)|go:"GO:0006412"(translation)|ensembl:YLL039C|rcsb pdb:1OTR|rcsb pdb:1Q0W|rcsb pdb:1WR1|rcsb pdb:1ZW7|rcsb pdb:2G3Q|go:"GO:0005634"(nucleus)|go:"GO:0006281"(DNA repair)|cygd:YIL148w|cygd:YKR094c|cygd:YLL039c|rcsb pdb:2JT4|rcsb pdb:2JWZ|rcsb pdb:3CMM|interpro:IPR019954|interpro:IPR019956|interpro:IPR019955|rcsb pdb:3L0W|rcsb pdb:3L10|rcsb pdb:3MHS|rcsb pdb:2KDI	-	crc64:8910804DF9156661	no-uniprot-update:obsolete protein	3d-r-factors:working 19.1%, free 24.7%|3d-resolution:2.70A|figure legend:Fig 1, Table 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/09/10	2014/10/16	rogid:5LkbO/GWqGTyuGWZG6biTGdbYII559292	rogid:sUFDb7kGedoC/k80bI4sjOaMLHs4932	intact-crc:F22243B789C62AA3|rigid:+Z0kXiCpKh56l8xVIhZtqdRI95I	false	sufficient binding region:10-1024	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P22515	intact:EBI-19797	intact:EBI-19703|uniprotkb:D6VWZ3|ensemblfungi:YKL210W	dip:DIP-1549N|uniprotkb:P04838|uniprotkb:Q6LA96	psi-mi:uba1_yeast(display_long)|uniprotkb:UBA1(gene name)|psi-mi:UBA1(display_short)|uniprotkb:YKL210W(locus name)	psi-mi:ubiq_yeast(display_short)|psi-mi:EBI-19797(display_long)|uniprotkb:UBI1(gene name)|psi-mi:UBI1(display_short)|uniprotkb:RPL40A(gene name synonym)|uniprotkb:YIL148W(locus name)|uniprotkb:UBI2(gene name)|psi-mi:UBI2(display_short)|uniprotkb:RPL40B(gene name synonym)|uniprotkb:YKR094C(locus name)|uniprotkb:UBI3(gene name)|psi-mi:UBI3(display_short)|uniprotkb:RPS31(gene name synonym)|uniprotkb:YLR167W(locus name)|uniprotkb:L9470.14(orf name)|uniprotkb:UBI4(gene name)|psi-mi:UBI4(display_short)|uniprotkb:SCD2(gene name synonym)|uniprotkb:YLL039C(locus name)	psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis)	Lee et al. (2008)	imex:IM-11908|pubmed:18662542	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-1808711|imex:IM-11908-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_012712.1|sgd:S000001693|ensemblfungi:YKL210W(gene)|ensemblfungi:YKL210W_mRNA(transcript)|go:"GO:0004839"(ubiquitin activating enzyme activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0006974"(cellular response to DNA damage stimulus)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0032446"(protein modification by small protein conjugation)|interpro:IPR000011(Ubiquitin-activating enzyme, E1)|interpro:IPR000594(UBA/THIF-type NAD/FAD binding fold)|interpro:IPR018074|interpro:IPR018075|interpro:IPR018965|interpro:IPR019572|interpro:IPR032418|interpro:IPR032420|interpro:IPR033127|interpro:IPR035985|interpro:IPR038252|interpro:IPR042063|interpro:IPR042302|interpro:IPR042449|rcsb pdb:3CMM|rcsb pdb:4NNJ|rcsb pdb:5L6H|rcsb pdb:5L6I|rcsb pdb:5L6J|rcsb pdb:5TR4|rcsb pdb:6NYA|rcsb pdb:6ZQH|rcsb pdb:7K5J|reactome:R-SCE-1169408|reactome:R-SCE-8866652|reactome:R-SCE-983168|dip:DIP-4853N|mint:P22515	sgd:S000001410(RPL40A)|sgd:S000001802(RPL40B)|sgd:S000004157(RPS31)|sgd:S000003962(UBI4)|uniprotkb:P61864(see-also)|refseq:NP_012118.1|refseq:NP_013020.1|refseq:NP_013061.1|refseq:NP_013268.1|ensembl:YIL148W|ensembl:YKR094C|ensembl:YLR167W|go:"GO:0005515"(protein binding)|interpro:IPR000626(Ubiquitin)|cygd:YLR167w|go:"GO:0022625"(cytosolic large ribosomal subunit)|go:"GO:0005739"(mitochondrion)|go:"GO:0003735"(structural constituent of ribosome)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0042254"(ribosome biogenesis)|go:"GO:0006412"(translation)|ensembl:YLL039C|rcsb pdb:1OTR|rcsb pdb:1Q0W|rcsb pdb:1WR1|rcsb pdb:1ZW7|rcsb pdb:2G3Q|go:"GO:0005634"(nucleus)|go:"GO:0006281"(DNA repair)|cygd:YIL148w|cygd:YKR094c|cygd:YLL039c|rcsb pdb:2JT4|rcsb pdb:2JWZ|rcsb pdb:3CMM|interpro:IPR019954|interpro:IPR019956|interpro:IPR019955|rcsb pdb:3L0W|rcsb pdb:3L10|rcsb pdb:3MHS|rcsb pdb:2KDI	psi-mi:"MI:0469"(intact)	crc64:8910804DF9156661	no-uniprot-update:obsolete protein	figure legend:Fig 3C, Supp. Fig. 3|comment:"A single charge reversal mutation (Glu1004Lys) inhibits the transthioesterification of the E2 enzyme Ubc1, with Asp1014Lys/glu1016lys double mutant and the corresponding triple mutant showing an even more pronounced reduction"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/09/10	2014/10/16	rogid:5LkbO/GWqGTyuGWZG6biTGdbYII559292	rogid:sUFDb7kGedoC/k80bI4sjOaMLHs4932	intact-crc:625D8D598E21DA5F|rigid:+Z0kXiCpKh56l8xVIhZtqdRI95I	false	sufficient binding region:10-1024	fluorescent dye label:?-?	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0867"(tag visualisation by fluorescence)
uniprotkb:P21734	intact:EBI-19797	intact:EBI-19717|uniprotkb:D6VSF9|ensemblfungi:YDR177W	dip:DIP-1549N|uniprotkb:P04838|uniprotkb:Q6LA96	psi-mi:ubc1_yeast(display_long)|uniprotkb:UBC1(gene name)|psi-mi:UBC1(display_short)|uniprotkb:YDR177W(locus name)|uniprotkb:YD9395.10(orf name)|uniprotkb:Ubiquitin-protein ligase(gene name synonym)|uniprotkb:Ubiquitin carrier protein(gene name synonym)|uniprotkb:Ubiquitin-conjugating enzyme E2-24 kDa(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme 1(gene name synonym)	psi-mi:ubiq_yeast(display_short)|psi-mi:EBI-19797(display_long)|uniprotkb:UBI1(gene name)|psi-mi:UBI1(display_short)|uniprotkb:RPL40A(gene name synonym)|uniprotkb:YIL148W(locus name)|uniprotkb:UBI2(gene name)|psi-mi:UBI2(display_short)|uniprotkb:RPL40B(gene name synonym)|uniprotkb:YKR094C(locus name)|uniprotkb:UBI3(gene name)|psi-mi:UBI3(display_short)|uniprotkb:RPS31(gene name synonym)|uniprotkb:YLR167W(locus name)|uniprotkb:L9470.14(orf name)|uniprotkb:UBI4(gene name)|psi-mi:UBI4(display_short)|uniprotkb:SCD2(gene name synonym)|uniprotkb:YLL039C(locus name)	psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis)	Lee et al. (2008)	imex:IM-11908|pubmed:18662542	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-1808874|imex:IM-11908-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000002584|ensemblfungi:YDR177W(gene)|ensemblfungi:YDR177W_mRNA(transcript)|go:"GO:0000209"(protein polyubiquitination)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0016050"(vesicle organization)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0043161"(proteasome-mediated ubiquitin-dependent protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070628"(proteasome binding)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR009060(UBA-like)|interpro:IPR015368(Ubiquitin-conjugating enzyme, C-terminal fungi)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:1FXT|rcsb pdb:1FZY|rcsb pdb:1TTE|reactome:R-SCE-8866652|reactome:R-SCE-983168|refseq:NP_010462.3|dip:DIP-6594N|mint:P21734	sgd:S000001410(RPL40A)|sgd:S000001802(RPL40B)|sgd:S000004157(RPS31)|sgd:S000003962(UBI4)|uniprotkb:P61864(see-also)|refseq:NP_012118.1|refseq:NP_013020.1|refseq:NP_013061.1|refseq:NP_013268.1|ensembl:YIL148W|ensembl:YKR094C|ensembl:YLR167W|go:"GO:0005515"(protein binding)|interpro:IPR000626(Ubiquitin)|cygd:YLR167w|go:"GO:0022625"(cytosolic large ribosomal subunit)|go:"GO:0005739"(mitochondrion)|go:"GO:0003735"(structural constituent of ribosome)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0042254"(ribosome biogenesis)|go:"GO:0006412"(translation)|ensembl:YLL039C|rcsb pdb:1OTR|rcsb pdb:1Q0W|rcsb pdb:1WR1|rcsb pdb:1ZW7|rcsb pdb:2G3Q|go:"GO:0005634"(nucleus)|go:"GO:0006281"(DNA repair)|cygd:YIL148w|cygd:YKR094c|cygd:YLL039c|rcsb pdb:2JT4|rcsb pdb:2JWZ|rcsb pdb:3CMM|interpro:IPR019954|interpro:IPR019956|interpro:IPR019955|rcsb pdb:3L0W|rcsb pdb:3L10|rcsb pdb:3MHS|rcsb pdb:2KDI	psi-mi:"MI:0469"(intact)	crc64:899CC397A1285145	no-uniprot-update:obsolete protein	figure legend:Fig 3C, Supp. Fig. 3|comment:"A single charge reversal mutation (Lys5Glu, Lys9Glu) inhibits the transthioesterification from the E1 enzyme Uba1, with the double mutant  double mutant showing an even more pronounced reduction"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/09/10	2014/10/16	rogid:3N1WHZJcgMeK0OglGpGI2m4XU8Q559292	rogid:sUFDb7kGedoC/k80bI4sjOaMLHs4932	intact-crc:C195CAF581B3DF26|rigid:Q6nX03uGlve6HQ8IWZK7AlwAEbs	false	-	fluorescent dye label:?-?	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0867"(tag visualisation by fluorescence)