#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P18064	uniprotkb:Q8H1F2	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	intact:EBI-1627025|uniprotkb:Q8RWS5|uniprotkb:Q9LU81|uniprotkb:Q9LU82|ensemblplants:AT3G26090.1	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:rgs1_arath(display_long)|uniprotkb:RGS1(gene name)|psi-mi:RGS1(display_short)|uniprotkb:MPE11.27(orf name)|uniprotkb:MPE11.28(orf name)|uniprotkb:At3g26090(locus name)	psi-mi:"MI:0055"(fluorescent resonance energy transfer)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1627031|imex:IM-9449-1|dip:DIP-88047E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0584"(fluorescence acceptor)	psi-mi:"MI:0583"(fluorescence donor)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	refseq:NP_189238.2|interpro:IPR016137(Regulator of G protein signalling superfamily)|mint:Q8H1F2|interpro:IPR036305|ensemblplants:AT3G26090(gene)|ensemblplants:AT3G26090.1(transcript)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005634"(nucleus)|go:"GO:0005886"(plasma membrane)|go:"GO:0009414"(response to water deprivation)|go:"GO:0009737"(response to abscisic acid)|go:"GO:0009749"(response to glucose)|go:"GO:0009867"(jasmonic acid mediated signaling pathway)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0010008"(endosome membrane)|go:"GO:0010182"(sugar mediated signaling pathway)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0043547"(positive regulation of GTPase activity)	psi-mi:"MI:0465"(dip)	-	-	figure legend:Figure 1B|comment:"The authors note that \"FRET signals were exclusively observed on or around plastids that were in close proximity to the plasma membrane\"."|antagonist:D-glucose|comment:"The GPA1 (P18064) containing construct consisted of the cyan fluorescent protein inserted into the loop between the predicted alpha A and alpha B helices (between alanine 97 and glutamine 98) of the protein."|comment:"The authors speculated that \"AtRGS1is a membrane receptor or coreceptorfor D-glucose that interacts with AtGPA1 upon glucose binding\"."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:3702(arath-root)|taxid:3702(Arabidopsis thaliana root tissue)	-	2008/01/25	2017/06/30	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	rogid:Y8ui9dAqlg0CFJeb6aXsEIv9nbE3702	intact-crc:D61E9FD9D6E084E1|rigid:6Qg4PMnpqPzjFUIbMWNaytb7e1E	false	cyan fluorescent protein tag:?-?	yellow fluorescent protein tag:?-?	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0867"(tag visualisation by fluorescence)
chebi:"CHEBI:43000"	uniprotkb:P18064	intact:EBI-1388758	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	psi-mi:guanosine 5'-[gamma-thio]triphosphate(display_short)|psi-mi:"CHEBI:43000"(display_long)	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:"MI:0405"(competition binding)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1627042|imex:IM-9449-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	-	-	-	figure legend:Table S1|comment:Only guanine nucleotide triphosphates were able to compete with GTP binding to GPA1.|comment:Guanosine triposphate was radiolabelled.|comment:"The authors noted that \"recombinant AtGPA1 had a high specific activity, binding [35S]GTP gamma S with a stoichiometry of 0.91 mol of GTP gammaS/mol of protein\"."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/01/25	2017/06/30	-	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	intact-crc:CD2D9AEF3B38F98C	false	-	his tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:15996"	uniprotkb:P18064	intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451"	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:"MI:0405"(competition binding)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1627042|imex:IM-9449-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0586"(inhibitor)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	-	author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2"	-	figure legend:Table S1|comment:Only guanine nucleotide triphosphates were able to compete with GTP binding to GPA1.|comment:Guanosine triposphate was radiolabelled.|comment:"The authors noted that \"recombinant AtGPA1 had a high specific activity, binding [35S]GTP gamma S with a stoichiometry of 0.91 mol of GTP gammaS/mol of protein\"."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/01/25	2017/06/30	-	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	intact-crc:CD2D9AEF3B38F98C	false	-	his tag:?-?	0	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:15996"	uniprotkb:P18064	intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451"	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:"MI:0017"(classical fluorescence spectroscopy)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1750035|imex:IM-9449-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	-	author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2"	-	figure legend:Figure 2B|comment:Guanosine triposphate was tagged with the the green-fluorescent BODIPY FL fluorophore.|kinetics:"The authors state that \"kobs, observed rate of GTPgS binding to G alpha GDP (equivalent to the rate of GDP release from G alpha because it is the rate-limiting step in the nucleotide exchange process)\" was 14.4 min per second."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/01/25	2017/06/30	-	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	intact-crc:990764A4685CF95E	false	-	his tag:?-?	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0867"(tag visualisation by fluorescence)
chebi:"CHEBI:15996"	uniprotkb:P18064	intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451"	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:"MI:0051"(fluorescence technology)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1750096|imex:IM-9449-6	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	-	author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2"	-	figure legend:Figure 2C Supplementary information Figure 5|comment:Guanosine triposphate was radiolabelled.|comment:The binding of guanosine triposphate was idenpendent of magnesium ion concentration over a range of 2 micromole to 20 millimole.|kinetics:"The authors state that \"kobs, observed rate of GTPgS binding to G alpha GDP (equivalent to the rate of GDP release from G alpha because it is the rate-limiting step in the nucleotide exchange process)\" was 8.7 min per second."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/01/25	2017/06/30	-	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	intact-crc:0BECFC00ECE6B48F	false	-	his tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:15996"	uniprotkb:P18064	intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451"	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:"MI:0419"(gtpase assay)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1750137|imex:IM-9449-7	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	-	author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2"	-	figure legend:Figure 2E|comment:Guanosine triposphate was radiolabelled with 32P.|kinetics:"The authors state that Kcat (rate of hydrolysis of bound GTP by enzyme) was 0.12 minute per second at 20 degrees celsius."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/01/25	2017/06/30	-	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	intact-crc:B178009D17716DE0	false	-	his tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:15996"	uniprotkb:P09471	intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451"	intact:EBI-715087|uniprotkb:P29777|uniprotkb:Q8TD72|uniprotkb:Q9UMV4|intact:EBI-2558210|ensembl:ENSP00000262493|ensembl:ENSP00000491223	psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:gnao_human(display_long)|uniprotkb:GNAO1(gene name)|psi-mi:GNAO1(display_short)	psi-mi:"MI:0049"(filter binding)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1777426|imex:IM-9449-8	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:NP_066268.1|refseq:NP_620073.2|ensembl:ENSG00000087258(gene)|ensembl:ENST00000262493(transcript)|ensembl:ENST00000638705(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006936"(muscle contraction)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007212"(dopamine receptor signaling pathway)|go:"GO:0007568"(aging)|go:"GO:0007626"(locomotory behavior)|go:"GO:0008016"(regulation of heart contraction)|go:"GO:0030900"(forebrain development)|go:"GO:0031175"(neuron projection development)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0031821"(G protein-coupled serotonin receptor binding)|go:"GO:0031852"(mu-type opioid receptor binding)|go:"GO:0030425"(dendrite)|go:"GO:0032794"(GTPase activating protein binding)|go:"GO:0034097"(response to cytokine)|go:"GO:0042475"(odontogenesis of dentin-containing tooth)|go:"GO:0042493"|go:"GO:0042542"(response to hydrogen peroxide)|go:"GO:0043209"(myelin sheath)|go:"GO:0043278"(response to morphine)|go:"GO:0043547"(positive regulation of GTPase activity)|go:"GO:0044297"(cell body)|go:"GO:0046872"(metal ion binding)|go:"GO:0051430"(corticotropin-releasing hormone receptor 1 binding)|go:"GO:0051926"(negative regulation of calcium ion transport)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR001408(G-protein alpha subunit, group I)|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|mint:P09471|rcsb pdb:6FUF|rcsb pdb:6G79|rcsb pdb:6K41|rcsb pdb:6OIK|rcsb pdb:6WWZ|rcsb pdb:7D76|rcsb pdb:7D77|reactome:R-HSA-4086398	-	author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2"	-	figure legend:Figure 2A|kinetics:The observed rate of [35S]GTP gamma S binding to AtGPA1K was Kobs = 0.08 per minute.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/01/25	2017/06/30	-	rogid:z55dsHfgpwFC/q0mtQHF3HkBMJo9606	intact-crc:C074B380EC5E971F	false	-	-	0	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:15996"	uniprotkb:P18064	intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451"	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:"MI:0049"(filter binding)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1777477|imex:IM-9449-9	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	-	author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2"	-	figure legend:Figure 2A|comment:"The authors noted that \"recombinant AtGPA1 had a high specific activity, binding [35S]GTP gamma S with a stoichiometry of 0.91 mol of GTP gammaS/mol of protein\"."|kinetics:The observed rate of [35S]GTP gamma S binding to AtGPA1K was Kobs = 1.4 per minute.|caution:"The observed rate of binding of GTP to protein was likely to be an underestimate. The authors state the observed rate is \" likely an underestimate because the first time point shows 75% binding in this assay format\"."|comment:GTP was radiolabelled.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/01/25	2017/06/30	-	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	intact-crc:B31B5DFB842DDBBC	false	-	his tag:?-?	0	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:15996"	uniprotkb:P18064	intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451"	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:"MI:0419"(gtpase assay)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1777504|imex:IM-9449-10	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	-	author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2"	-	figure legend:Figure 2F Supplementary Information Figure 7 Supplementary Information Figure 8|comment:Guanosine triposphate was radiolabelled with 32P.|kinetics:The authors state that rate of GTP hydrolysis was 0.063 per minute at steady state at 20 degrees celsius.|comment:"The authors noted that \"a 5-fold molar excess of AtRGS1 gave a 35-fold increase in steady-state GTPase activity\"."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/01/25	2017/06/30	-	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	intact-crc:10DBA575A31282D0	false	-	his tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P18064	uniprotkb:Q8H1F2	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	intact:EBI-1627025|uniprotkb:Q8RWS5|uniprotkb:Q9LU81|uniprotkb:Q9LU82|ensemblplants:AT3G26090.1	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:rgs1_arath(display_long)|uniprotkb:RGS1(gene name)|psi-mi:RGS1(display_short)|uniprotkb:MPE11.27(orf name)|uniprotkb:MPE11.28(orf name)|uniprotkb:At3g26090(locus name)	psi-mi:"MI:0419"(gtpase assay)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1777504|imex:IM-9449-10	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	refseq:NP_189238.2|interpro:IPR016137(Regulator of G protein signalling superfamily)|mint:Q8H1F2|interpro:IPR036305|ensemblplants:AT3G26090(gene)|ensemblplants:AT3G26090.1(transcript)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005634"(nucleus)|go:"GO:0005886"(plasma membrane)|go:"GO:0009414"(response to water deprivation)|go:"GO:0009737"(response to abscisic acid)|go:"GO:0009749"(response to glucose)|go:"GO:0009867"(jasmonic acid mediated signaling pathway)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0010008"(endosome membrane)|go:"GO:0010182"(sugar mediated signaling pathway)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0043547"(positive regulation of GTPase activity)	-	-	-	figure legend:Figure 2F Supplementary Information Figure 7 Supplementary Information Figure 8|comment:Guanosine triposphate was radiolabelled with 32P.|kinetics:The authors state that rate of GTP hydrolysis was 0.063 per minute at steady state at 20 degrees celsius.|comment:"The authors noted that \"a 5-fold molar excess of AtRGS1 gave a 35-fold increase in steady-state GTPase activity\"."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/01/25	2017/06/30	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	rogid:Y8ui9dAqlg0CFJeb6aXsEIv9nbE3702	intact-crc:10DBA575A31282D0	false	his tag:?-?	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:15996"	uniprotkb:P18064	intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451"	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:"MI:0419"(gtpase assay)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0883"(gtpase reaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1777516|imex:IM-9449-11	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	-	author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2"	-	figure legend:Figure 3A|comment:Guanosine triposphate was radiolabelled with 32P.|comment:"The c-terminal region of Q8H1F2 was used in this study (from 249 to 459 amino acid residues). It was demonstrated that mutation of the glutamic acid residue at position 320 in Q8H1F2 to a lysine residue abolished the stimulatory effect of the protein on GTPase activity of GPA1 (P18064)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/01/25	2017/06/30	-	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	intact-crc:D81DFAE87EF040B1	false	-	his tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P18064	uniprotkb:Q8H1F2	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	intact:EBI-1627025|uniprotkb:Q8RWS5|uniprotkb:Q9LU81|uniprotkb:Q9LU82|ensemblplants:AT3G26090.1	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:rgs1_arath(display_long)|uniprotkb:RGS1(gene name)|psi-mi:RGS1(display_short)|uniprotkb:MPE11.27(orf name)|uniprotkb:MPE11.28(orf name)|uniprotkb:At3g26090(locus name)	psi-mi:"MI:0419"(gtpase assay)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0883"(gtpase reaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1777516|imex:IM-9449-11	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	refseq:NP_189238.2|interpro:IPR016137(Regulator of G protein signalling superfamily)|mint:Q8H1F2|interpro:IPR036305|ensemblplants:AT3G26090(gene)|ensemblplants:AT3G26090.1(transcript)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005634"(nucleus)|go:"GO:0005886"(plasma membrane)|go:"GO:0009414"(response to water deprivation)|go:"GO:0009737"(response to abscisic acid)|go:"GO:0009749"(response to glucose)|go:"GO:0009867"(jasmonic acid mediated signaling pathway)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0010008"(endosome membrane)|go:"GO:0010182"(sugar mediated signaling pathway)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0043547"(positive regulation of GTPase activity)	-	-	-	figure legend:Figure 3A|comment:Guanosine triposphate was radiolabelled with 32P.|comment:"The c-terminal region of Q8H1F2 was used in this study (from 249 to 459 amino acid residues). It was demonstrated that mutation of the glutamic acid residue at position 320 in Q8H1F2 to a lysine residue abolished the stimulatory effect of the protein on GTPase activity of GPA1 (P18064)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/01/25	2017/06/30	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	rogid:Y8ui9dAqlg0CFJeb6aXsEIv9nbE3702	intact-crc:D81DFAE87EF040B1	false	his tag:?-?	glutathione s tranferase tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:15996"	uniprotkb:P18064	intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451"	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:"MI:0107"(surface plasmon resonance)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1777544|imex:IM-9449-2|dip:DIP-88047E	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	psi-mi:"MI:0465"(dip)	author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2"	-	figure legend:Figure 3B|comment:GST-AtRGS1 was immobilized on a biosensor surface.|comment:"AtRGS1(wild-type) bound AtGPA1 in the transition state for GTP hydrolysis"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/01/25	2017/06/30	-	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	intact-crc:8F675B80E5103D97	false	-	his tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P18064	uniprotkb:Q8H1F2	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	intact:EBI-1627025|uniprotkb:Q8RWS5|uniprotkb:Q9LU81|uniprotkb:Q9LU82|ensemblplants:AT3G26090.1	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:rgs1_arath(display_long)|uniprotkb:RGS1(gene name)|psi-mi:RGS1(display_short)|uniprotkb:MPE11.27(orf name)|uniprotkb:MPE11.28(orf name)|uniprotkb:At3g26090(locus name)	psi-mi:"MI:0107"(surface plasmon resonance)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1777544|imex:IM-9449-2|dip:DIP-88047E	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	refseq:NP_189238.2|interpro:IPR016137(Regulator of G protein signalling superfamily)|mint:Q8H1F2|interpro:IPR036305|ensemblplants:AT3G26090(gene)|ensemblplants:AT3G26090.1(transcript)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005634"(nucleus)|go:"GO:0005886"(plasma membrane)|go:"GO:0009414"(response to water deprivation)|go:"GO:0009737"(response to abscisic acid)|go:"GO:0009749"(response to glucose)|go:"GO:0009867"(jasmonic acid mediated signaling pathway)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0010008"(endosome membrane)|go:"GO:0010182"(sugar mediated signaling pathway)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0043547"(positive regulation of GTPase activity)	psi-mi:"MI:0465"(dip)	-	-	figure legend:Figure 3B|comment:GST-AtRGS1 was immobilized on a biosensor surface.|comment:"AtRGS1(wild-type) bound AtGPA1 in the transition state for GTP hydrolysis"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/01/25	2017/06/30	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	rogid:Y8ui9dAqlg0CFJeb6aXsEIv9nbE3702	intact-crc:8F675B80E5103D97	false	his tag:?-?	glutathione s tranferase tag:?-?|mutation disrupting interaction:320-320	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:51987"	uniprotkb:Q8H1F2	intact:EBI-1777616	intact:EBI-1627025|uniprotkb:Q8RWS5|uniprotkb:Q9LU81|uniprotkb:Q9LU82|ensemblplants:AT3G26090.1	psi-mi:gdp-tetrafluoroaluminate(display_short)|psi-mi:"CHEBI:51987"(display_long)	psi-mi:rgs1_arath(display_long)|uniprotkb:RGS1(gene name)|psi-mi:RGS1(display_short)|uniprotkb:MPE11.27(orf name)|uniprotkb:MPE11.28(orf name)|uniprotkb:At3g26090(locus name)	psi-mi:"MI:0096"(pull down)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1777567|imex:IM-9449-3|dip:DIP-88047E	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0684"(ancillary)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:NP_189238.2|interpro:IPR016137(Regulator of G protein signalling superfamily)|mint:Q8H1F2|interpro:IPR036305|ensemblplants:AT3G26090(gene)|ensemblplants:AT3G26090.1(transcript)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005634"(nucleus)|go:"GO:0005886"(plasma membrane)|go:"GO:0009414"(response to water deprivation)|go:"GO:0009737"(response to abscisic acid)|go:"GO:0009749"(response to glucose)|go:"GO:0009867"(jasmonic acid mediated signaling pathway)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0010008"(endosome membrane)|go:"GO:0010182"(sugar mediated signaling pathway)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0043547"(positive regulation of GTPase activity)	psi-mi:"MI:0465"(dip)	comment:GDP.AlF4 is a transition stsate analogue	-	figure legend:Supplementary Information Figure 9|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/01/25	2017/06/30	-	rogid:Y8ui9dAqlg0CFJeb6aXsEIv9nbE3702	intact-crc:0C3BB6F8D8FF621C	false	-	glutathione s tranferase tag:?-?|mutation disrupting interaction:320-320|sufficient binding region:249-459	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q8H1F2	uniprotkb:P18064	intact:EBI-1627025|uniprotkb:Q8RWS5|uniprotkb:Q9LU81|uniprotkb:Q9LU82|ensemblplants:AT3G26090.1	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	psi-mi:rgs1_arath(display_long)|uniprotkb:RGS1(gene name)|psi-mi:RGS1(display_short)|uniprotkb:MPE11.27(orf name)|uniprotkb:MPE11.28(orf name)|uniprotkb:At3g26090(locus name)	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:"MI:0096"(pull down)	Johnston et al. (2007)	pubmed:17951432|imex:IM-9449	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-1777567|imex:IM-9449-3|dip:DIP-88047E	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_189238.2|interpro:IPR016137(Regulator of G protein signalling superfamily)|mint:Q8H1F2|interpro:IPR036305|ensemblplants:AT3G26090(gene)|ensemblplants:AT3G26090.1(transcript)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005634"(nucleus)|go:"GO:0005886"(plasma membrane)|go:"GO:0009414"(response to water deprivation)|go:"GO:0009737"(response to abscisic acid)|go:"GO:0009749"(response to glucose)|go:"GO:0009867"(jasmonic acid mediated signaling pathway)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0010008"(endosome membrane)|go:"GO:0010182"(sugar mediated signaling pathway)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0043547"(positive regulation of GTPase activity)	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	psi-mi:"MI:0465"(dip)	-	-	figure legend:Supplementary Information Figure 9|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2008/01/25	2017/06/30	rogid:Y8ui9dAqlg0CFJeb6aXsEIv9nbE3702	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	intact-crc:0C3BB6F8D8FF621C	false	glutathione s tranferase tag:?-?|mutation disrupting interaction:320-320|sufficient binding region:249-459	his tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)