#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:F4IEM5 uniprotkb:P18064 intact:EBI-1804974|uniprotkb:Q9C929|ensemblplants:AT1G52920.1 intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2 psi-mi:gcr2_arath(display_long)|uniprotkb:GCR2(gene name)|psi-mi:GCR2(display_short)|uniprotkb:G-protein coupled receptor 2(gene name synonym)|uniprotkb:GPCR(gene name synonym)|uniprotkb:F14G24.19(orf name)|uniprotkb:At1g52920(locus name) psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name) psi-mi:"MI:0107"(surface plasmon resonance) Liu et al. (2007) pubmed:17347412 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-1804984 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:3T33|interpro:IPR007822(Lanthionine synthetase C-like)|interpro:IPR020464|refseq:NP_175700.2|interpro:IPR012341(Six-hairpin glycosidase)|ensemblplants:AT1G52920(gene)|ensemblplants:AT1G52920.1(transcript)|go:"GO:0005886"(plasma membrane)|go:"GO:0005975"(carbohydrate metabolic process)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009787"(regulation of abscisic acid-activated signaling pathway)|go:"GO:0010231"(maintenance of seed dormancy)|go:"GO:0010427"(abscisic acid binding)|go:"GO:0019898"(extrinsic component of membrane)|go:"GO:0046872"(metal ion binding) ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N - - - figure legend:Supplementary Figure 3|kinetics:"The dissociation binding constant (Kd) 2.1 x 10-9 M" taxid:-1(in vitro)|taxid:-1(In vitro) - 2008/08/26 2014/10/16 rogid:jScWpqe+7jc21MVaBWDkJMpPCQg3702 rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702 intact-crc:6D3C4CE5D01EE6F9|rigid:nY9xOjKx9MXKVLBElVNSsi0kRSc false his tag:n-n his tag:n-n - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P18064 uniprotkb:F4IEM5 intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2 intact:EBI-1804974|uniprotkb:Q9C929|ensemblplants:AT1G52920.1 psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name) psi-mi:gcr2_arath(display_long)|uniprotkb:GCR2(gene name)|psi-mi:GCR2(display_short)|uniprotkb:G-protein coupled receptor 2(gene name synonym)|uniprotkb:GPCR(gene name synonym)|uniprotkb:F14G24.19(orf name)|uniprotkb:At1g52920(locus name) psi-mi:"MI:0112"(ubiquitin reconstruction) Liu et al. (2007) pubmed:17347412 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-1805002 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N rcsb pdb:3T33|interpro:IPR007822(Lanthionine synthetase C-like)|interpro:IPR020464|refseq:NP_175700.2|interpro:IPR012341(Six-hairpin glycosidase)|ensemblplants:AT1G52920(gene)|ensemblplants:AT1G52920.1(transcript)|go:"GO:0005886"(plasma membrane)|go:"GO:0005975"(carbohydrate metabolic process)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009787"(regulation of abscisic acid-activated signaling pathway)|go:"GO:0010231"(maintenance of seed dormancy)|go:"GO:0010427"(abscisic acid binding)|go:"GO:0019898"(extrinsic component of membrane)|go:"GO:0046872"(metal ion binding) - - - figure legend:Supplementary Figure 4 Figure 3E|inhibition:"Abscisic acid (active form)" taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2008/08/26 2014/10/16 rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702 rogid:jScWpqe+7jc21MVaBWDkJMpPCQg3702 intact-crc:876F770B9B9EA7E6|rigid:nY9xOjKx9MXKVLBElVNSsi0kRSc false - necessary binding region:299-410 - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P18064 uniprotkb:O04714 intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2 intact:EBI-443899|ensemblplants:AT1G48270.1|ensemblplants:AT1G48270.2|uniprotkb:O04214|uniprotkb:Q8LPG0 psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name) psi-mi:gcr1_arath(display_long)|uniprotkb:GCR1(gene name)|psi-mi:GCR1(display_short)|uniprotkb:F11A17.17(orf name)|uniprotkb:At1g48270(locus name) psi-mi:"MI:0112"(ubiquitin reconstruction) Liu et al. (2007) pubmed:17347412 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-1805007 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N ensemblplants:AT1G48270(gene)|ensemblplants:AT1G48270.1(transcript)|ensemblplants:AT1G48270.2(transcript)|go:"GO:0000278"(mitotic cell cycle)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005774"(vacuolar membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0006629"(lipid metabolic process)|go:"GO:0007166"(cell surface receptor signaling pathway)|go:"GO:0007202"(activation of phospholipase C activity)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009735"(response to cytokinin)|go:"GO:0009736"(cytokinin-activated signaling pathway)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009742"(brassinosteroid mediated signaling pathway)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009788"(negative regulation of abscisic acid-activated signaling pathway)|go:"GO:0009908"(flower development)|go:"GO:0009939"(positive regulation of gibberellic acid mediated signaling pathway)|go:"GO:0010231"(maintenance of seed dormancy)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0032960"(regulation of inositol trisphosphate biosynthetic process)|go:"GO:0044214"(spanning component of plasma membrane)|refseq:NP_175261.1|interpro:IPR022343|interpro:IPR017981|interpro:IPR022340|refseq:NP_001321802.1 - - - figure legend:Supplementary Figure 4 taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2008/08/26 2014/10/16 rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702 rogid:vSfyBqWC0VWqPaklrzE0zO9loWA3702 intact-crc:4B90456C71CACEB4|rigid:vMmzbxBsIGuwD0jdZ1uyherzG1A false - - - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:F4IEM5 uniprotkb:P18064 intact:EBI-1804974|uniprotkb:Q9C929|ensemblplants:AT1G52920.1 intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2 psi-mi:gcr2_arath(display_long)|uniprotkb:GCR2(gene name)|psi-mi:GCR2(display_short)|uniprotkb:G-protein coupled receptor 2(gene name synonym)|uniprotkb:GPCR(gene name synonym)|uniprotkb:F14G24.19(orf name)|uniprotkb:At1g52920(locus name) psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name) psi-mi:"MI:0809"(bimolecular fluorescence complementation) Liu et al. (2007) pubmed:17347412 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-1805021 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:3T33|interpro:IPR007822(Lanthionine synthetase C-like)|interpro:IPR020464|refseq:NP_175700.2|interpro:IPR012341(Six-hairpin glycosidase)|ensemblplants:AT1G52920(gene)|ensemblplants:AT1G52920.1(transcript)|go:"GO:0005886"(plasma membrane)|go:"GO:0005975"(carbohydrate metabolic process)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009787"(regulation of abscisic acid-activated signaling pathway)|go:"GO:0010231"(maintenance of seed dormancy)|go:"GO:0010427"(abscisic acid binding)|go:"GO:0019898"(extrinsic component of membrane)|go:"GO:0046872"(metal ion binding) ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N - - - figure legend:Figure 1A taxid:3702(arath-mesophyll)|taxid:3702(Arabidopsis thaliana mesophyll) - 2008/08/26 2014/10/16 rogid:jScWpqe+7jc21MVaBWDkJMpPCQg3702 rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702 intact-crc:44700569F6A6149F|rigid:nY9xOjKx9MXKVLBElVNSsi0kRSc false ha tag:c-c|yellow fluorescent protein tag:?-?|necessary binding region:299-410 myc tag:?-?|yellow fluorescent protein tag:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P18064 uniprotkb:F4IEM5 intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2 intact:EBI-1804974|uniprotkb:Q9C929|ensemblplants:AT1G52920.1 psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name) psi-mi:gcr2_arath(display_long)|uniprotkb:GCR2(gene name)|psi-mi:GCR2(display_short)|uniprotkb:G-protein coupled receptor 2(gene name synonym)|uniprotkb:GPCR(gene name synonym)|uniprotkb:F14G24.19(orf name)|uniprotkb:At1g52920(locus name) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Liu et al. (2007) pubmed:17347412 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-1805045 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N rcsb pdb:3T33|interpro:IPR007822(Lanthionine synthetase C-like)|interpro:IPR020464|refseq:NP_175700.2|interpro:IPR012341(Six-hairpin glycosidase)|ensemblplants:AT1G52920(gene)|ensemblplants:AT1G52920.1(transcript)|go:"GO:0005886"(plasma membrane)|go:"GO:0005975"(carbohydrate metabolic process)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009787"(regulation of abscisic acid-activated signaling pathway)|go:"GO:0010231"(maintenance of seed dormancy)|go:"GO:0010427"(abscisic acid binding)|go:"GO:0019898"(extrinsic component of membrane)|go:"GO:0046872"(metal ion binding) - - - figure legend:Figure 1C taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") - 2008/08/26 2014/10/16 rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702 rogid:jScWpqe+7jc21MVaBWDkJMpPCQg3702 intact-crc:2D78296D285DA932|rigid:nY9xOjKx9MXKVLBElVNSsi0kRSc false - flag tag:?-? - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) chebi:"CHEBI:22152" uniprotkb:F4IEM5 intact:EBI-1805052 intact:EBI-1804974|uniprotkb:Q9C929|ensemblplants:AT1G52920.1 psi-mi:abscisic acid(display_short)|psi-mi:"CHEBI:22152"(display_long) psi-mi:gcr2_arath(display_long)|uniprotkb:GCR2(gene name)|psi-mi:GCR2(display_short)|uniprotkb:G-protein coupled receptor 2(gene name synonym)|uniprotkb:GPCR(gene name synonym)|uniprotkb:F14G24.19(orf name)|uniprotkb:At1g52920(locus name) psi-mi:"MI:0440"(saturation binding) Liu et al. (2007) pubmed:17347412 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-1805064 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0328"(small molecule) psi-mi:"MI:0326"(protein) - rcsb pdb:3T33|interpro:IPR007822(Lanthionine synthetase C-like)|interpro:IPR020464|refseq:NP_175700.2|interpro:IPR012341(Six-hairpin glycosidase)|ensemblplants:AT1G52920(gene)|ensemblplants:AT1G52920.1(transcript)|go:"GO:0005886"(plasma membrane)|go:"GO:0005975"(carbohydrate metabolic process)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009787"(regulation of abscisic acid-activated signaling pathway)|go:"GO:0010231"(maintenance of seed dormancy)|go:"GO:0010427"(abscisic acid binding)|go:"GO:0019898"(extrinsic component of membrane)|go:"GO:0046872"(metal ion binding) - comment:"abscisic acid (CHEBI:22152) is a apo carotenoid sesquiterpenoids (CHEBI:36758) , abscisic acid (CHEBI:22152) is a monocarboxylic acids (CHEBI:25384)"|comment:"IUPAC name (2Z,4E)-5-(1-hydroxy-2,6,6-trimethyl-4-oxocyclohex-2-en-1-yl)-3-methylpenta-2,4-dienoic acid" - figure legend:Figure 3 Supplementary Figure 9|comment:"The authors concluded \"GCR2 binding of ABA is stereospecific\"."|kinetics:"Maximum binding is 0.8 mol mol-1 protein with Kd = 20.1 nM (mean +/- SD; n = 5)."|comment:"The authors demonstrated \"The binding of ABA to GCR2 is pH-dependent\". Binding was highest at PH 7.5."|kinetics:"Dissociation constant (Kd) = 20.1 nM (mean +/- SD; n = 5)." taxid:-1(in vitro)|taxid:-1(In vitro) - 2008/08/26 2014/10/16 - rogid:jScWpqe+7jc21MVaBWDkJMpPCQg3702 intact-crc:ED5BF28C86635DD9 false - his tag:n-n - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:F4IEM5 uniprotkb:P18064 intact:EBI-1804974|uniprotkb:Q9C929|ensemblplants:AT1G52920.1 intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2 psi-mi:gcr2_arath(display_long)|uniprotkb:GCR2(gene name)|psi-mi:GCR2(display_short)|uniprotkb:G-protein coupled receptor 2(gene name synonym)|uniprotkb:GPCR(gene name synonym)|uniprotkb:F14G24.19(orf name)|uniprotkb:At1g52920(locus name) psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Liu et al. (2007) pubmed:17347412 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-1805073 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:3T33|interpro:IPR007822(Lanthionine synthetase C-like)|interpro:IPR020464|refseq:NP_175700.2|interpro:IPR012341(Six-hairpin glycosidase)|ensemblplants:AT1G52920(gene)|ensemblplants:AT1G52920.1(transcript)|go:"GO:0005886"(plasma membrane)|go:"GO:0005975"(carbohydrate metabolic process)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009787"(regulation of abscisic acid-activated signaling pathway)|go:"GO:0010231"(maintenance of seed dormancy)|go:"GO:0010427"(abscisic acid binding)|go:"GO:0019898"(extrinsic component of membrane)|go:"GO:0046872"(metal ion binding) ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N - - - figure legend:Figure 3E|antagonist:"(+) ABA (abscisic acid)"|comment:"Application of the physiologically active form of ABA [(+/-)-ABA or (+)-ABA] indeed disrupted the GCR2-GPA1 interaction, whereas the physiologically inactive forms of ABA analogs [(-)-ABA or trans-ABA] did not affect the interaction between GCR2 and GPA1, as indicated in the coimmunoprecipitation assay. The authors concluded that \" this result again clearly confirmed that the binding of ABA to GCR2 is stereospecific\"." taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2008/08/26 2014/10/16 rogid:jScWpqe+7jc21MVaBWDkJMpPCQg3702 rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702 intact-crc:ED89AFFA8D53A69C|rigid:nY9xOjKx9MXKVLBElVNSsi0kRSc false ha tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot)