#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P26200 uniprotkb:Q54N00 intact:EBI-1808610|uniprotkb:Q54M07|ensemblprotists:EAL64269 intact:EBI-1808560|uniprotkb:Q5TJB8|ensemblprotists:EAL64632 psi-mi:prof2_dicdi(display_long)|uniprotkb:proB(gene name)|psi-mi:proB(display_short)|uniprotkb:Profilin II(gene name synonym)|uniprotkb:DDB_G0286187(orf name) psi-mi:forh_dicdi(display_long)|uniprotkb:forH(gene name)|psi-mi:forH(display_short)|uniprotkb:dia2(gene name synonym)|uniprotkb:drf2(gene name synonym)|uniprotkb:DDB_G0285589(orf name)|uniprotkb:Diaphanous-related formin dia2(gene name synonym) psi-mi:"MI:0018"(two hybrid) Schirenbeck et al. (2005) pubmed:15908944 taxid:44689(dicdi)|taxid:44689("Dictyostelium discoideum (Slime mold)") taxid:44689(dicdi)|taxid:44689("Dictyostelium discoideum (Slime mold)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-1808608 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) dictybase:DDB_G0286187|ensemblprotists:DDB_G0286187(gene)|ensemblprotists:EAL64269(transcript)|go:"GO:0000281"(mitotic cytokinesis)|go:"GO:0003779"(actin binding)|go:"GO:0003785"(actin monomer binding)|go:"GO:0005938"(cell cortex)|go:"GO:0006887"(exocytosis)|go:"GO:0006907"(pinocytosis)|go:"GO:0006972"(hyperosmotic response)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0030587"(sorocarp development)|go:"GO:0030837"(negative regulation of actin filament polymerization)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:0031012"(extracellular matrix)|go:"GO:0032037"(myosin I heavy chain binding)|go:"GO:0033299"(secretion of lysosomal enzymes)|go:"GO:0042989"(sequestering of actin monomers)|go:"GO:0045010"(actin nucleation)|go:"GO:0048870"(cell motility)|go:"GO:0050765"(negative regulation of phagocytosis)|interpro:IPR005455(Profilin, plant)|interpro:IPR027310|interpro:IPR036140|refseq:XP_637824.1 dictybase:DDB_G0285589|ensemblprotists:EAL64632(transcript)|go:"GO:0000902"(cell morphogenesis)|go:"GO:0005522"(profilin binding)|go:"GO:0005938"(cell cortex)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030175"(filopodium)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:0030866"(cortical actin cytoskeleton organization)|go:"GO:0031254"(cell trailing edge)|go:"GO:0031267"(small GTPase binding)|go:"GO:0031268"(pseudopodium organization)|go:"GO:0031589"(cell-substrate adhesion)|go:"GO:0032433"(filopodium tip)|go:"GO:0045010"(actin nucleation)|go:"GO:0046847"(filopodium assembly)|go:"GO:0048870"(cell motility)|go:"GO:0051015"(actin filament binding)|go:"GO:0051638"(barbed-end actin filament uncapping)|go:"GO:0070060"('de novo' actin filament nucleation)|interpro:IPR010472(Diaphanous FH3)|interpro:IPR010473(Diaphanous GTPase-binding)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR014768(GTPase-binding/formin homology 3)|interpro:IPR015425(Actin-binding FH2)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR042201|refseq:XP_638143.1|dip:DIP-46561N|ensemblprotists:DDB_G0285589(gene) - - - figure legend:Fig 1c taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2008/09/10 2014/10/16 rogid:T3YSeipyYwtFfqj2uOn6OtDNPPI44689 rogid:SGV+z4C5tJ+OrBQh48J0d/fQbcU44689 intact-crc:C529194D9CA65EF5|rigid:v0qEUQBLTR9M2I1Om1EL/1tJlBU false - sufficient binding region:589-615 - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P34144 uniprotkb:Q54N00 intact:EBI-1808643|uniprotkb:Q54YK6|uniprotkb:Q9GPU0|ensemblprotists:EAL68107 intact:EBI-1808560|uniprotkb:Q5TJB8|ensemblprotists:EAL64632 psi-mi:rac1a_dicdi(display_long)|uniprotkb:rac1A(gene name)|psi-mi:rac1A(display_short)|uniprotkb:DDB_G0277869(orf name) psi-mi:forh_dicdi(display_long)|uniprotkb:forH(gene name)|psi-mi:forH(display_short)|uniprotkb:dia2(gene name synonym)|uniprotkb:drf2(gene name synonym)|uniprotkb:DDB_G0285589(orf name)|uniprotkb:Diaphanous-related formin dia2(gene name synonym) psi-mi:"MI:0096"(pull down) Schirenbeck et al. (2005) pubmed:15908944 taxid:44689(dicdi)|taxid:44689("Dictyostelium discoideum (Slime mold)") taxid:44689(dicdi)|taxid:44689("Dictyostelium discoideum (Slime mold)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-1808638 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) dictybase:DDB_G0277869|ensemblprotists:DDB_G0277869(gene)|ensemblprotists:EAL68107(transcript)|go:"GO:0000281"(mitotic cytokinesis)|go:"GO:0001891"(phagocytic cup)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005811"(lipid droplet)|go:"GO:0005856"(cytoskeleton)|go:"GO:0005886"(plasma membrane)|go:"GO:0005938"(cell cortex)|go:"GO:0007015"(actin filament organization)|go:"GO:0007163"(establishment or maintenance of cell polarity)|go:"GO:0008360"(regulation of cell shape)|go:"GO:0016601"(Rac protein signal transduction)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:0030865"(cortical cytoskeleton organization)|go:"GO:0031254"(cell trailing edge)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032956"(regulation of actin cytoskeleton organization)|go:"GO:0042995"(cell projection)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043652"(engulfment of apoptotic cell)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:1902412"(regulation of mitotic cytokinesis)|go:"GO:1904269"(cell leading edge cell cortex)|go:"GO:2000145"(regulation of cell motility)|interpro:IPR001806(Ras GTPase)|interpro:IPR003578(Ras small GTPase, Rho type)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR027417|reactome:R-DDI-193648|reactome:R-DDI-2029482|reactome:R-DDI-389359|reactome:R-DDI-3928662|reactome:R-DDI-445144|reactome:R-DDI-5626467|reactome:R-DDI-5627123|reactome:R-DDI-5663213|reactome:R-DDI-5668599|reactome:R-DDI-5687128|reactome:R-DDI-6798695|reactome:R-DDI-9013149|reactome:R-DDI-9013404|reactome:R-DDI-9013407|reactome:R-DDI-9013418|reactome:R-DDI-9013420|reactome:R-DDI-9013422|reactome:R-DDI-9013424|reactome:R-DDI-9706019|reactome:R-DDI-983231|refseq:XP_642196.1 dictybase:DDB_G0285589|ensemblprotists:EAL64632(transcript)|go:"GO:0000902"(cell morphogenesis)|go:"GO:0005522"(profilin binding)|go:"GO:0005938"(cell cortex)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030175"(filopodium)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:0030866"(cortical actin cytoskeleton organization)|go:"GO:0031254"(cell trailing edge)|go:"GO:0031267"(small GTPase binding)|go:"GO:0031268"(pseudopodium organization)|go:"GO:0031589"(cell-substrate adhesion)|go:"GO:0032433"(filopodium tip)|go:"GO:0045010"(actin nucleation)|go:"GO:0046847"(filopodium assembly)|go:"GO:0048870"(cell motility)|go:"GO:0051015"(actin filament binding)|go:"GO:0051638"(barbed-end actin filament uncapping)|go:"GO:0070060"('de novo' actin filament nucleation)|interpro:IPR010472(Diaphanous FH3)|interpro:IPR010473(Diaphanous GTPase-binding)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR014768(GTPase-binding/formin homology 3)|interpro:IPR015425(Actin-binding FH2)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR042201|refseq:XP_638143.1|dip:DIP-46561N|ensemblprotists:DDB_G0285589(gene) - - - figure legend:Fig 1d|stimulant:Interaction between rac1A and forH is stimulated by GTP-gamma-S. taxid:-1(in vitro)|taxid:-1(In vitro) - 2008/09/10 2014/10/16 rogid:kBmhXb5GJdKRREo3zRPPLA/xf2c44689 rogid:SGV+z4C5tJ+OrBQh48J0d/fQbcU44689 intact-crc:306BCF778A482F86|rigid:erd4USwMln2lupoX8aOtRwQPTK0 false glutathione s tranferase tag:n-n green fluorescent protein tag:n-n - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q54K32 uniprotkb:P34144 intact:EBI-1808670|uniprotkb:O15815|uniprotkb:P90531|intact:EBI-1997884|ensemblprotists:EAL63601 intact:EBI-1808643|uniprotkb:Q54YK6|uniprotkb:Q9GPU0|ensemblprotists:EAL68107 psi-mi:rgaa_dicdi(display_long)|uniprotkb:rgaA(gene name)|psi-mi:rgaA(display_short)|uniprotkb:DDB_G0287585(orf name)|uniprotkb:DG1029(gene name synonym)|uniprotkb:rasGAP1(gene name synonym)|uniprotkb:Developmental gene 1029 protein(gene name synonym) psi-mi:rac1a_dicdi(display_long)|uniprotkb:rac1A(gene name)|psi-mi:rac1A(display_short)|uniprotkb:DDB_G0277869(orf name) psi-mi:"MI:0096"(pull down) Schirenbeck et al. (2005) pubmed:15908944 taxid:44689(dicdi)|taxid:44689("Dictyostelium discoideum (Slime mold)") taxid:44689(dicdi)|taxid:44689("Dictyostelium discoideum (Slime mold)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-1808662 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) dictybase:DDB_G0287585|go:"GO:0007165"(signal transduction)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030435"(sporulation resulting in formation of a cellular spore)|go:"GO:0030587"(sorocarp development)|go:"GO:0031149"(sorocarp stalk cell differentiation)|go:"GO:0031154"(culmination involved in sorocarp development)|go:"GO:0031254"(cell trailing edge)|go:"GO:0031255"(lateral part of motile cell)|go:"GO:0031267"(small GTPase binding)|go:"GO:0032154"(cleavage furrow)|go:"GO:0032956"(regulation of actin cytoskeleton organization)|go:"GO:0034613"(cellular protein localization)|go:"GO:0036360"(sorocarp stalk morphogenesis)|go:"GO:0043087"(regulation of GTPase activity)|go:"GO:0043327"(chemotaxis to cAMP)|go:"GO:0043406"(positive regulation of MAP kinase activity)|go:"GO:0045180"(basal cortex)|go:"GO:0048870"(cell motility)|go:"GO:0051015"(actin filament binding)|go:"GO:0051495"(positive regulation of cytoskeleton organization)|go:"GO:0140509"(epithelium-like organization)|go:"GO:1903665"(negative regulation of asexual reproduction)|interpro:IPR000593(RasGAP protein, C-terminal)|interpro:IPR001936(Ras GTPase-activating protein)|interpro:IPR008936(Rho GTPase activation protein)|reactome:R-DDI-5626467|reactome:R-DDI-5674135|reactome:R-DDI-6798695|reactome:R-DDI-9013149|reactome:R-DDI-9013404|reactome:R-DDI-9013420|reactome:R-DDI-9013424|refseq:XP_637132.1|ensemblprotists:DDB_G0287585(gene)|ensemblprotists:EAL63601(transcript)|go:"GO:0000281"(mitotic cytokinesis)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005516"(calmodulin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0005938"(cell cortex) dictybase:DDB_G0277869|ensemblprotists:DDB_G0277869(gene)|ensemblprotists:EAL68107(transcript)|go:"GO:0000281"(mitotic cytokinesis)|go:"GO:0001891"(phagocytic cup)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005811"(lipid droplet)|go:"GO:0005856"(cytoskeleton)|go:"GO:0005886"(plasma membrane)|go:"GO:0005938"(cell cortex)|go:"GO:0007015"(actin filament organization)|go:"GO:0007163"(establishment or maintenance of cell polarity)|go:"GO:0008360"(regulation of cell shape)|go:"GO:0016601"(Rac protein signal transduction)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:0030865"(cortical cytoskeleton organization)|go:"GO:0031254"(cell trailing edge)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032956"(regulation of actin cytoskeleton organization)|go:"GO:0042995"(cell projection)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043652"(engulfment of apoptotic cell)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:1902412"(regulation of mitotic cytokinesis)|go:"GO:1904269"(cell leading edge cell cortex)|go:"GO:2000145"(regulation of cell motility)|interpro:IPR001806(Ras GTPase)|interpro:IPR003578(Ras small GTPase, Rho type)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR027417|reactome:R-DDI-193648|reactome:R-DDI-2029482|reactome:R-DDI-389359|reactome:R-DDI-3928662|reactome:R-DDI-445144|reactome:R-DDI-5626467|reactome:R-DDI-5627123|reactome:R-DDI-5663213|reactome:R-DDI-5668599|reactome:R-DDI-5687128|reactome:R-DDI-6798695|reactome:R-DDI-9013149|reactome:R-DDI-9013404|reactome:R-DDI-9013407|reactome:R-DDI-9013418|reactome:R-DDI-9013420|reactome:R-DDI-9013422|reactome:R-DDI-9013424|reactome:R-DDI-9706019|reactome:R-DDI-983231|refseq:XP_642196.1 - - - figure legend:Fig 1d|stimulant:Interaction between rac1A and rgaA is stimulated by GTP-gamma-S. taxid:-1(in vitro)|taxid:-1(In vitro) - 2008/09/10 2014/10/16 rogid:4XjyZq0wzx6CBoAiZ+82m3JQrWg44689 rogid:kBmhXb5GJdKRREo3zRPPLA/xf2c44689 intact-crc:C45FB8339A29086B|rigid:ZsUKCMG3kl3axYkdJ9rXflqKvcY false - glutathione s tranferase tag:n-n - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P34144 uniprotkb:Q54N00 intact:EBI-1808643|uniprotkb:Q54YK6|uniprotkb:Q9GPU0|ensemblprotists:EAL68107 intact:EBI-1808560|uniprotkb:Q5TJB8|ensemblprotists:EAL64632 psi-mi:rac1a_dicdi(display_long)|uniprotkb:rac1A(gene name)|psi-mi:rac1A(display_short)|uniprotkb:DDB_G0277869(orf name) psi-mi:forh_dicdi(display_long)|uniprotkb:forH(gene name)|psi-mi:forH(display_short)|uniprotkb:dia2(gene name synonym)|uniprotkb:drf2(gene name synonym)|uniprotkb:DDB_G0285589(orf name)|uniprotkb:Diaphanous-related formin dia2(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Schirenbeck et al. (2005) pubmed:15908944 taxid:44689(dicdi)|taxid:44689("Dictyostelium discoideum (Slime mold)") taxid:44689(dicdi)|taxid:44689("Dictyostelium discoideum (Slime mold)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-1808691 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) dictybase:DDB_G0277869|ensemblprotists:DDB_G0277869(gene)|ensemblprotists:EAL68107(transcript)|go:"GO:0000281"(mitotic cytokinesis)|go:"GO:0001891"(phagocytic cup)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005811"(lipid droplet)|go:"GO:0005856"(cytoskeleton)|go:"GO:0005886"(plasma membrane)|go:"GO:0005938"(cell cortex)|go:"GO:0007015"(actin filament organization)|go:"GO:0007163"(establishment or maintenance of cell polarity)|go:"GO:0008360"(regulation of cell shape)|go:"GO:0016601"(Rac protein signal transduction)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:0030865"(cortical cytoskeleton organization)|go:"GO:0031254"(cell trailing edge)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032956"(regulation of actin cytoskeleton organization)|go:"GO:0042995"(cell projection)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043652"(engulfment of apoptotic cell)|go:"GO:0045335"(phagocytic vesicle)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:1902412"(regulation of mitotic cytokinesis)|go:"GO:1904269"(cell leading edge cell cortex)|go:"GO:2000145"(regulation of cell motility)|interpro:IPR001806(Ras GTPase)|interpro:IPR003578(Ras small GTPase, Rho type)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR027417|reactome:R-DDI-193648|reactome:R-DDI-2029482|reactome:R-DDI-389359|reactome:R-DDI-3928662|reactome:R-DDI-445144|reactome:R-DDI-5626467|reactome:R-DDI-5627123|reactome:R-DDI-5663213|reactome:R-DDI-5668599|reactome:R-DDI-5687128|reactome:R-DDI-6798695|reactome:R-DDI-9013149|reactome:R-DDI-9013404|reactome:R-DDI-9013407|reactome:R-DDI-9013418|reactome:R-DDI-9013420|reactome:R-DDI-9013422|reactome:R-DDI-9013424|reactome:R-DDI-9706019|reactome:R-DDI-983231|refseq:XP_642196.1 dictybase:DDB_G0285589|ensemblprotists:EAL64632(transcript)|go:"GO:0000902"(cell morphogenesis)|go:"GO:0005522"(profilin binding)|go:"GO:0005938"(cell cortex)|go:"GO:0015629"(actin cytoskeleton)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030175"(filopodium)|go:"GO:0030838"(positive regulation of actin filament polymerization)|go:"GO:0030866"(cortical actin cytoskeleton organization)|go:"GO:0031254"(cell trailing edge)|go:"GO:0031267"(small GTPase binding)|go:"GO:0031268"(pseudopodium organization)|go:"GO:0031589"(cell-substrate adhesion)|go:"GO:0032433"(filopodium tip)|go:"GO:0045010"(actin nucleation)|go:"GO:0046847"(filopodium assembly)|go:"GO:0048870"(cell motility)|go:"GO:0051015"(actin filament binding)|go:"GO:0051638"(barbed-end actin filament uncapping)|go:"GO:0070060"('de novo' actin filament nucleation)|interpro:IPR010472(Diaphanous FH3)|interpro:IPR010473(Diaphanous GTPase-binding)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR014768(GTPase-binding/formin homology 3)|interpro:IPR015425(Actin-binding FH2)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR042201|refseq:XP_638143.1|dip:DIP-46561N|ensemblprotists:DDB_G0285589(gene) - - - figure legend:Fig 1e taxid:44689(dicdi)|taxid:44689("Dictyostelium discoideum (Slime mold)") - 2008/09/10 2014/10/16 rogid:kBmhXb5GJdKRREo3zRPPLA/xf2c44689 rogid:SGV+z4C5tJ+OrBQh48J0d/fQbcU44689 intact-crc:02F530FAC02F6A9E|rigid:erd4USwMln2lupoX8aOtRwQPTK0 false green fluorescent protein tag:n-n|mutation disrupting interaction:17-17|mutation decreasing interaction:12-12 - - - psi-mi:"MI:0705"(anti tag western blot) psi-mi:"MI:0705"(anti tag western blot)