#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B chebi:"CHEBI:15996" uniprotkb:O23680 intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451" intact:EBI-639377|uniprotkb:Q9GDD3|uniprotkb:Q94B42|ensemblplants:AT1G02280.1|ensemblplants:AT1G02280.2 psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name) psi-mi:toc33_arath(display_long)|uniprotkb:TOC33(gene name)|psi-mi:TOC33(display_short)|uniprotkb:T7I23.11(orf name)|uniprotkb:At1g02280(locus name)|uniprotkb:PPI1(gene name synonym)|uniprotkb:33 kDa chloroplast outer envelope protein(gene name synonym)|uniprotkb:Plastid protein import 1(gene name synonym) psi-mi:"MI:0049"(filter binding) Weibel et al. (2003) pubmed:12869544 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-1766430 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0328"(small molecule) psi-mi:"MI:0326"(protein) - rcsb pdb:3DEF|refseq:NP_001117215.1|refseq:NP_171730.1|interpro:IPR006703(AIG1)|interpro:IPR005688(Chloroplast protein import component Toc34)|rcsb pdb:2J3E|rcsb pdb:3BB3|rcsb pdb:3BB4|ensemblplants:AT1G02280.1(transcript)|ensemblplants:AT1G02280.2(transcript)|go:"GO:0003924"(GTPase activity)|ensemblplants:AT1G02280(gene)|go:"GO:0005525"(GTP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0009507"(chloroplast)|go:"GO:0009707"(chloroplast outer membrane)|go:"GO:0009941"(chloroplast envelope)|go:"GO:0015450"(protein-transporting ATPase activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0045036"(protein targeting to chloroplast)|go:"GO:0046872"(metal ion binding)|interpro:IPR027417 - author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2" - figure legend:Figure 2|comment:GTP was radiolabelled with alpha P32|comment:"The authors conclude that \"Toc33-wt and Toc33-R130A bind GTP with high affinity\"." taxid:-1(in vitro)|taxid:-1(In vitro) - 2008/04/16 2016/08/15 - rogid:EjXVNXS1fT9oWn77HiaV4702vVo3702 intact-crc:3AA4C78FEE0F7659 false - sufficient binding region:1-265|his tag:c-c - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) chebi:"CHEBI:15996" uniprotkb:O23680 intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451" intact:EBI-639377|uniprotkb:Q9GDD3|uniprotkb:Q94B42|ensemblplants:AT1G02280.1|ensemblplants:AT1G02280.2 psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name) psi-mi:toc33_arath(display_long)|uniprotkb:TOC33(gene name)|psi-mi:TOC33(display_short)|uniprotkb:T7I23.11(orf name)|uniprotkb:At1g02280(locus name)|uniprotkb:PPI1(gene name synonym)|uniprotkb:33 kDa chloroplast outer envelope protein(gene name synonym)|uniprotkb:Plastid protein import 1(gene name synonym) psi-mi:"MI:0405"(competition binding) Weibel et al. (2003) pubmed:12869544 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-1766448 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0328"(small molecule) psi-mi:"MI:0326"(protein) - rcsb pdb:3DEF|refseq:NP_001117215.1|refseq:NP_171730.1|interpro:IPR006703(AIG1)|interpro:IPR005688(Chloroplast protein import component Toc34)|rcsb pdb:2J3E|rcsb pdb:3BB3|rcsb pdb:3BB4|ensemblplants:AT1G02280.1(transcript)|ensemblplants:AT1G02280.2(transcript)|go:"GO:0003924"(GTPase activity)|ensemblplants:AT1G02280(gene)|go:"GO:0005525"(GTP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0009507"(chloroplast)|go:"GO:0009707"(chloroplast outer membrane)|go:"GO:0009941"(chloroplast envelope)|go:"GO:0015450"(protein-transporting ATPase activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0045036"(protein targeting to chloroplast)|go:"GO:0046872"(metal ion binding)|interpro:IPR027417 - author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2" - figure legend:Figure 2B|comment:GTP was radiolabelled with alpha P32|comment:"The authors conclude that \"the data suggest that GTP-binding to Toc33 may be saturated at cellular concentrations of GTP (>= 0.1 mM) \"."|inhibition:GDP|inhibition:GTP|kinetics:"Dissociation constant (Kd) 2.6 micromolar wild type Toc33 and Kd 3.8 micromolar Toc33 mutant form Arg130Ala." taxid:-1(in vitro)|taxid:-1(In vitro) - 2008/04/16 2016/08/15 - rogid:EjXVNXS1fT9oWn77HiaV4702vVo3702 intact-crc:09EB914A67CEA16B false - sufficient binding region:1-265|his tag:c-c|mutation decreasing interaction:130-130 - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) chebi:"CHEBI:17552" uniprotkb:O23680 intact:EBI-1265684 intact:EBI-639377|uniprotkb:Q9GDD3|uniprotkb:Q94B42|ensemblplants:AT1G02280.1|ensemblplants:AT1G02280.2 psi-mi:gdp(display_short)|psi-mi:"CHEBI:17552"(display_long) psi-mi:toc33_arath(display_long)|uniprotkb:TOC33(gene name)|psi-mi:TOC33(display_short)|uniprotkb:T7I23.11(orf name)|uniprotkb:At1g02280(locus name)|uniprotkb:PPI1(gene name synonym)|uniprotkb:33 kDa chloroplast outer envelope protein(gene name synonym)|uniprotkb:Plastid protein import 1(gene name synonym) psi-mi:"MI:0405"(competition binding) Weibel et al. (2003) pubmed:12869544 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-1766464 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0328"(small molecule) psi-mi:"MI:0326"(protein) - rcsb pdb:3DEF|refseq:NP_001117215.1|refseq:NP_171730.1|interpro:IPR006703(AIG1)|interpro:IPR005688(Chloroplast protein import component Toc34)|rcsb pdb:2J3E|rcsb pdb:3BB3|rcsb pdb:3BB4|ensemblplants:AT1G02280.1(transcript)|ensemblplants:AT1G02280.2(transcript)|go:"GO:0003924"(GTPase activity)|ensemblplants:AT1G02280(gene)|go:"GO:0005525"(GTP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0009507"(chloroplast)|go:"GO:0009707"(chloroplast outer membrane)|go:"GO:0009941"(chloroplast envelope)|go:"GO:0015450"(protein-transporting ATPase activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0045036"(protein targeting to chloroplast)|go:"GO:0046872"(metal ion binding)|interpro:IPR027417 - - - figure legend:Figure 2B|comment:GTP was radiolabelled with alpha P32|inhibition:GDP|inhibition:GTP|kinetics:"Dissociation constant (Kd) 4.5 micromolar wild type Toc33 and Kd 8.8 micromolar Toc33 mutant form Arg130Ala." taxid:-1(in vitro)|taxid:-1(In vitro) - 2008/04/16 2014/10/16 - rogid:EjXVNXS1fT9oWn77HiaV4702vVo3702 intact-crc:99DC995C2EDABC4A false - sufficient binding region:1-265|his tag:c-c|mutation decreasing interaction:130-130 - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) chebi:"CHEBI:15996" uniprotkb:O23680 intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451" intact:EBI-639377|uniprotkb:Q9GDD3|uniprotkb:Q94B42|ensemblplants:AT1G02280.1|ensemblplants:AT1G02280.2 psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name) psi-mi:toc33_arath(display_long)|uniprotkb:TOC33(gene name)|psi-mi:TOC33(display_short)|uniprotkb:T7I23.11(orf name)|uniprotkb:At1g02280(locus name)|uniprotkb:PPI1(gene name synonym)|uniprotkb:33 kDa chloroplast outer envelope protein(gene name synonym)|uniprotkb:Plastid protein import 1(gene name synonym) psi-mi:"MI:0419"(gtpase assay) Weibel et al. (2003) pubmed:12869544 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-1766488 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0328"(small molecule) psi-mi:"MI:0326"(protein) - rcsb pdb:3DEF|refseq:NP_001117215.1|refseq:NP_171730.1|interpro:IPR006703(AIG1)|interpro:IPR005688(Chloroplast protein import component Toc34)|rcsb pdb:2J3E|rcsb pdb:3BB3|rcsb pdb:3BB4|ensemblplants:AT1G02280.1(transcript)|ensemblplants:AT1G02280.2(transcript)|go:"GO:0003924"(GTPase activity)|ensemblplants:AT1G02280(gene)|go:"GO:0005525"(GTP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0009507"(chloroplast)|go:"GO:0009707"(chloroplast outer membrane)|go:"GO:0009941"(chloroplast envelope)|go:"GO:0015450"(protein-transporting ATPase activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0045036"(protein targeting to chloroplast)|go:"GO:0046872"(metal ion binding)|interpro:IPR027417 - author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2" - figure legend:Figure 3|comment:GTP was radiolabelled with alpha P32|comment:"The authors conclude that \"both Toc33-wt (Fig. 3A, lanes 1�4) and Toc33-R130A (Fig. 3A, lanes 5�8) hydrolyze GTP, at a low concentration, with similar efficiencies. \"."|kinetics:"First-order rate constant, (Kcat) 0.013 per minute micromolar wild type Toc33." taxid:-1(in vitro)|taxid:-1(In vitro) - 2008/04/16 2016/08/15 - rogid:EjXVNXS1fT9oWn77HiaV4702vVo3702 intact-crc:5BDD563424124453 false - sufficient binding region:1-265|his tag:c-c - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) uniprotkb:O23680 uniprotkb:O23680 intact:EBI-639377|uniprotkb:Q9GDD3|uniprotkb:Q94B42|ensemblplants:AT1G02280.1|ensemblplants:AT1G02280.2 intact:EBI-639377|uniprotkb:Q9GDD3|uniprotkb:Q94B42|ensemblplants:AT1G02280.1|ensemblplants:AT1G02280.2 psi-mi:toc33_arath(display_long)|uniprotkb:TOC33(gene name)|psi-mi:TOC33(display_short)|uniprotkb:T7I23.11(orf name)|uniprotkb:At1g02280(locus name)|uniprotkb:PPI1(gene name synonym)|uniprotkb:33 kDa chloroplast outer envelope protein(gene name synonym)|uniprotkb:Plastid protein import 1(gene name synonym) psi-mi:toc33_arath(display_long)|uniprotkb:TOC33(gene name)|psi-mi:TOC33(display_short)|uniprotkb:T7I23.11(orf name)|uniprotkb:At1g02280(locus name)|uniprotkb:PPI1(gene name synonym)|uniprotkb:33 kDa chloroplast outer envelope protein(gene name synonym)|uniprotkb:Plastid protein import 1(gene name synonym) psi-mi:"MI:0276"(blue native page) Weibel et al. (2003) pubmed:12869544 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-1766508 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:3DEF|refseq:NP_001117215.1|refseq:NP_171730.1|interpro:IPR006703(AIG1)|interpro:IPR005688(Chloroplast protein import component Toc34)|rcsb pdb:2J3E|rcsb pdb:3BB3|rcsb pdb:3BB4|ensemblplants:AT1G02280.1(transcript)|ensemblplants:AT1G02280.2(transcript)|go:"GO:0003924"(GTPase activity)|ensemblplants:AT1G02280(gene)|go:"GO:0005525"(GTP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0009507"(chloroplast)|go:"GO:0009707"(chloroplast outer membrane)|go:"GO:0009941"(chloroplast envelope)|go:"GO:0015450"(protein-transporting ATPase activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0045036"(protein targeting to chloroplast)|go:"GO:0046872"(metal ion binding)|interpro:IPR027417 rcsb pdb:3DEF|refseq:NP_001117215.1|refseq:NP_171730.1|interpro:IPR006703(AIG1)|interpro:IPR005688(Chloroplast protein import component Toc34)|rcsb pdb:2J3E|rcsb pdb:3BB3|rcsb pdb:3BB4|ensemblplants:AT1G02280.1(transcript)|ensemblplants:AT1G02280.2(transcript)|go:"GO:0003924"(GTPase activity)|ensemblplants:AT1G02280(gene)|go:"GO:0005525"(GTP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0009507"(chloroplast)|go:"GO:0009707"(chloroplast outer membrane)|go:"GO:0009941"(chloroplast envelope)|go:"GO:0015450"(protein-transporting ATPase activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0045036"(protein targeting to chloroplast)|go:"GO:0046872"(metal ion binding)|interpro:IPR027417 - - - figure legend:Figure 4A taxid:-1(in vitro)|taxid:-1(In vitro) - 2008/04/16 2014/10/16 rogid:EjXVNXS1fT9oWn77HiaV4702vVo3702 rogid:EjXVNXS1fT9oWn77HiaV4702vVo3702 intact-crc:346EBD98628B8C10|rigid:r7gMU9secLfs2vpV8GoVC1UKR/E false sufficient binding region:1-265|his tag:c-c|mutation decreasing interaction:130-130 his tag:c-c|sufficient binding region:1-265|mutation decreasing interaction:130-130 - - psi-mi:"MI:0818"(molecular weight estimation by coomasie staining) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining) uniprotkb:O23680 uniprotkb:O23680 intact:EBI-639377|uniprotkb:Q9GDD3|uniprotkb:Q94B42|ensemblplants:AT1G02280.1|ensemblplants:AT1G02280.2 intact:EBI-639377|uniprotkb:Q9GDD3|uniprotkb:Q94B42|ensemblplants:AT1G02280.1|ensemblplants:AT1G02280.2 psi-mi:toc33_arath(display_long)|uniprotkb:TOC33(gene name)|psi-mi:TOC33(display_short)|uniprotkb:T7I23.11(orf name)|uniprotkb:At1g02280(locus name)|uniprotkb:PPI1(gene name synonym)|uniprotkb:33 kDa chloroplast outer envelope protein(gene name synonym)|uniprotkb:Plastid protein import 1(gene name synonym) psi-mi:toc33_arath(display_long)|uniprotkb:TOC33(gene name)|psi-mi:TOC33(display_short)|uniprotkb:T7I23.11(orf name)|uniprotkb:At1g02280(locus name)|uniprotkb:PPI1(gene name synonym)|uniprotkb:33 kDa chloroplast outer envelope protein(gene name synonym)|uniprotkb:Plastid protein import 1(gene name synonym) psi-mi:"MI:0276"(blue native page) Weibel et al. (2003) pubmed:12869544 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-1766531 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:3DEF|refseq:NP_001117215.1|refseq:NP_171730.1|interpro:IPR006703(AIG1)|interpro:IPR005688(Chloroplast protein import component Toc34)|rcsb pdb:2J3E|rcsb pdb:3BB3|rcsb pdb:3BB4|ensemblplants:AT1G02280.1(transcript)|ensemblplants:AT1G02280.2(transcript)|go:"GO:0003924"(GTPase activity)|ensemblplants:AT1G02280(gene)|go:"GO:0005525"(GTP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0009507"(chloroplast)|go:"GO:0009707"(chloroplast outer membrane)|go:"GO:0009941"(chloroplast envelope)|go:"GO:0015450"(protein-transporting ATPase activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0045036"(protein targeting to chloroplast)|go:"GO:0046872"(metal ion binding)|interpro:IPR027417 rcsb pdb:3DEF|refseq:NP_001117215.1|refseq:NP_171730.1|interpro:IPR006703(AIG1)|interpro:IPR005688(Chloroplast protein import component Toc34)|rcsb pdb:2J3E|rcsb pdb:3BB3|rcsb pdb:3BB4|ensemblplants:AT1G02280.1(transcript)|ensemblplants:AT1G02280.2(transcript)|go:"GO:0003924"(GTPase activity)|ensemblplants:AT1G02280(gene)|go:"GO:0005525"(GTP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0009507"(chloroplast)|go:"GO:0009707"(chloroplast outer membrane)|go:"GO:0009941"(chloroplast envelope)|go:"GO:0015450"(protein-transporting ATPase activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0045036"(protein targeting to chloroplast)|go:"GO:0046872"(metal ion binding)|interpro:IPR027417 - - - figure legend:Figure 4B taxid:-1(in vitro)|taxid:-1(In vitro) - 2008/04/16 2014/10/16 rogid:EjXVNXS1fT9oWn77HiaV4702vVo3702 rogid:EjXVNXS1fT9oWn77HiaV4702vVo3702 intact-crc:55E847858D89A691|rigid:r7gMU9secLfs2vpV8GoVC1UKR/E false his tag:c-c|sufficient binding region:1-265|mutation decreasing interaction:130-130 sufficient binding region:1-265|his tag:c-c|mutation decreasing interaction:130-130 - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O23680 uniprotkb:O23680 intact:EBI-639377|uniprotkb:Q9GDD3|uniprotkb:Q94B42|ensemblplants:AT1G02280.1|ensemblplants:AT1G02280.2 intact:EBI-639377|uniprotkb:Q9GDD3|uniprotkb:Q94B42|ensemblplants:AT1G02280.1|ensemblplants:AT1G02280.2 psi-mi:toc33_arath(display_long)|uniprotkb:TOC33(gene name)|psi-mi:TOC33(display_short)|uniprotkb:T7I23.11(orf name)|uniprotkb:At1g02280(locus name)|uniprotkb:PPI1(gene name synonym)|uniprotkb:33 kDa chloroplast outer envelope protein(gene name synonym)|uniprotkb:Plastid protein import 1(gene name synonym) psi-mi:toc33_arath(display_long)|uniprotkb:TOC33(gene name)|psi-mi:TOC33(display_short)|uniprotkb:T7I23.11(orf name)|uniprotkb:At1g02280(locus name)|uniprotkb:PPI1(gene name synonym)|uniprotkb:33 kDa chloroplast outer envelope protein(gene name synonym)|uniprotkb:Plastid protein import 1(gene name synonym) psi-mi:"MI:0276"(blue native page) Weibel et al. (2003) pubmed:12869544 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-1766555 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:3DEF|refseq:NP_001117215.1|refseq:NP_171730.1|interpro:IPR006703(AIG1)|interpro:IPR005688(Chloroplast protein import component Toc34)|rcsb pdb:2J3E|rcsb pdb:3BB3|rcsb pdb:3BB4|ensemblplants:AT1G02280.1(transcript)|ensemblplants:AT1G02280.2(transcript)|go:"GO:0003924"(GTPase activity)|ensemblplants:AT1G02280(gene)|go:"GO:0005525"(GTP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0009507"(chloroplast)|go:"GO:0009707"(chloroplast outer membrane)|go:"GO:0009941"(chloroplast envelope)|go:"GO:0015450"(protein-transporting ATPase activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0045036"(protein targeting to chloroplast)|go:"GO:0046872"(metal ion binding)|interpro:IPR027417 rcsb pdb:3DEF|refseq:NP_001117215.1|refseq:NP_171730.1|interpro:IPR006703(AIG1)|interpro:IPR005688(Chloroplast protein import component Toc34)|rcsb pdb:2J3E|rcsb pdb:3BB3|rcsb pdb:3BB4|ensemblplants:AT1G02280.1(transcript)|ensemblplants:AT1G02280.2(transcript)|go:"GO:0003924"(GTPase activity)|ensemblplants:AT1G02280(gene)|go:"GO:0005525"(GTP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0009507"(chloroplast)|go:"GO:0009707"(chloroplast outer membrane)|go:"GO:0009941"(chloroplast envelope)|go:"GO:0015450"(protein-transporting ATPase activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0045036"(protein targeting to chloroplast)|go:"GO:0046872"(metal ion binding)|interpro:IPR027417 - - - figure legend:Figure 4B taxid:-1(in vitro)|taxid:-1(In vitro) - 2008/04/16 2014/10/16 rogid:EjXVNXS1fT9oWn77HiaV4702vVo3702 rogid:EjXVNXS1fT9oWn77HiaV4702vVo3702 intact-crc:F68EC743DF59C0C5|rigid:r7gMU9secLfs2vpV8GoVC1UKR/E false sufficient binding region:1-265|his tag:c-c|mutation decreasing interaction:130-130 his tag:c-c|sufficient binding region:1-265|mutation decreasing interaction:130-130 - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) uniprotkb:O23680 uniprotkb:O23680 intact:EBI-639377|uniprotkb:Q9GDD3|uniprotkb:Q94B42|ensemblplants:AT1G02280.1|ensemblplants:AT1G02280.2 intact:EBI-639377|uniprotkb:Q9GDD3|uniprotkb:Q94B42|ensemblplants:AT1G02280.1|ensemblplants:AT1G02280.2 psi-mi:toc33_arath(display_long)|uniprotkb:TOC33(gene name)|psi-mi:TOC33(display_short)|uniprotkb:T7I23.11(orf name)|uniprotkb:At1g02280(locus name)|uniprotkb:PPI1(gene name synonym)|uniprotkb:33 kDa chloroplast outer envelope protein(gene name synonym)|uniprotkb:Plastid protein import 1(gene name synonym) psi-mi:toc33_arath(display_long)|uniprotkb:TOC33(gene name)|psi-mi:TOC33(display_short)|uniprotkb:T7I23.11(orf name)|uniprotkb:At1g02280(locus name)|uniprotkb:PPI1(gene name synonym)|uniprotkb:33 kDa chloroplast outer envelope protein(gene name synonym)|uniprotkb:Plastid protein import 1(gene name synonym) psi-mi:"MI:0096"(pull down) Weibel et al. (2003) pubmed:12869544 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-1766578 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:3DEF|refseq:NP_001117215.1|refseq:NP_171730.1|interpro:IPR006703(AIG1)|interpro:IPR005688(Chloroplast protein import component Toc34)|rcsb pdb:2J3E|rcsb pdb:3BB3|rcsb pdb:3BB4|ensemblplants:AT1G02280.1(transcript)|ensemblplants:AT1G02280.2(transcript)|go:"GO:0003924"(GTPase activity)|ensemblplants:AT1G02280(gene)|go:"GO:0005525"(GTP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0009507"(chloroplast)|go:"GO:0009707"(chloroplast outer membrane)|go:"GO:0009941"(chloroplast envelope)|go:"GO:0015450"(protein-transporting ATPase activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0045036"(protein targeting to chloroplast)|go:"GO:0046872"(metal ion binding)|interpro:IPR027417 rcsb pdb:3DEF|refseq:NP_001117215.1|refseq:NP_171730.1|interpro:IPR006703(AIG1)|interpro:IPR005688(Chloroplast protein import component Toc34)|rcsb pdb:2J3E|rcsb pdb:3BB3|rcsb pdb:3BB4|ensemblplants:AT1G02280.1(transcript)|ensemblplants:AT1G02280.2(transcript)|go:"GO:0003924"(GTPase activity)|ensemblplants:AT1G02280(gene)|go:"GO:0005525"(GTP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0009507"(chloroplast)|go:"GO:0009707"(chloroplast outer membrane)|go:"GO:0009941"(chloroplast envelope)|go:"GO:0015450"(protein-transporting ATPase activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0045036"(protein targeting to chloroplast)|go:"GO:0046872"(metal ion binding)|interpro:IPR027417 - - - figure legend:Figure 5 taxid:-1(in vitro)|taxid:-1(In vitro) - 2008/04/16 2014/10/16 rogid:EjXVNXS1fT9oWn77HiaV4702vVo3702 rogid:EjXVNXS1fT9oWn77HiaV4702vVo3702 intact-crc:68EF0695C314BC03|rigid:r7gMU9secLfs2vpV8GoVC1UKR/E false his tag:c-c|sufficient binding region:1-265 sufficient binding region:1-265|mutation decreasing interaction:130-130|35s radiolabel:?-? - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography) uniprotkb:O81283 uniprotkb:O23680 intact:EBI-639063|uniprotkb:O22774|uniprotkb:Q56W83|uniprotkb:Q945M4|ensemblplants:AT4G02510.1|ensemblplants:AT4G02510.2|ensemblplants:AT4G02510.3|ensemblplants:AT4G02510.4 intact:EBI-639377|uniprotkb:Q9GDD3|uniprotkb:Q94B42|ensemblplants:AT1G02280.1|ensemblplants:AT1G02280.2 psi-mi:tc159_arath(display_long)|uniprotkb:T14P8.24(orf name)|uniprotkb:At4g02510(locus name)|uniprotkb:TOC159(gene name)|psi-mi:TOC159(display_short)|uniprotkb:PPI2(gene name synonym)|uniprotkb:TOC160(gene name synonym)|uniprotkb:TOC86(gene name synonym)|uniprotkb:Translocase of chloroplast 160, chloroplastic(gene name synonym)|uniprotkb:Translocase of chloroplast 86, chloroplastic(gene name synonym)|uniprotkb:159 kDa chloroplast outer envelope protein(gene name synonym)|uniprotkb:Plastid protein import 2(gene name synonym)|uniprotkb:T10P11.19(orf name) psi-mi:toc33_arath(display_long)|uniprotkb:TOC33(gene name)|psi-mi:TOC33(display_short)|uniprotkb:T7I23.11(orf name)|uniprotkb:At1g02280(locus name)|uniprotkb:PPI1(gene name synonym)|uniprotkb:33 kDa chloroplast outer envelope protein(gene name synonym)|uniprotkb:Plastid protein import 1(gene name synonym) psi-mi:"MI:0096"(pull down) Weibel et al. (2003) pubmed:12869544 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-1766591 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_567242.2|interpro:IPR006703(AIG1)|interpro:IPR024283|interpro:IPR005690(Chloroplast protein import component Toc86/159)|interpro:IPR027417|ensemblplants:AT4G02510(gene)|ensemblplants:AT4G02510.1(transcript)|ensemblplants:AT4G02510.2(transcript)|ensemblplants:AT4G02510.3(transcript)|ensemblplants:AT4G02510.4(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005829"(cytosol)|go:"GO:0009507"(chloroplast)|go:"GO:0009536"(plastid)|go:"GO:0009707"(chloroplast outer membrane)|go:"GO:0009941"(chloroplast envelope)|go:"GO:0016021"(integral component of membrane)|go:"GO:0043024"(ribosomal small subunit binding)|go:"GO:0045036"(protein targeting to chloroplast)|go:"GO:0045037"(protein import into chloroplast stroma)|go:"GO:0046872"(metal ion binding)|go:"GO:0051087"(chaperone binding)|go:"GO:0061927"(TOC-TIC supercomplex I)|refseq:NP_001319848.1|refseq:NP_001328635.1|refseq:NP_001328636.1|mint:O81283 rcsb pdb:3DEF|refseq:NP_001117215.1|refseq:NP_171730.1|interpro:IPR006703(AIG1)|interpro:IPR005688(Chloroplast protein import component Toc34)|rcsb pdb:2J3E|rcsb pdb:3BB3|rcsb pdb:3BB4|ensemblplants:AT1G02280.1(transcript)|ensemblplants:AT1G02280.2(transcript)|go:"GO:0003924"(GTPase activity)|ensemblplants:AT1G02280(gene)|go:"GO:0005525"(GTP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0009507"(chloroplast)|go:"GO:0009707"(chloroplast outer membrane)|go:"GO:0009941"(chloroplast envelope)|go:"GO:0015450"(protein-transporting ATPase activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0045036"(protein targeting to chloroplast)|go:"GO:0046872"(metal ion binding)|interpro:IPR027417 - - - figure legend:Figure 6 taxid:-1(in vitro)|taxid:-1(In vitro) - 2008/04/16 2014/10/16 rogid:M3nIMktwNu5T3zDIErFXyqozvoo3702 rogid:EjXVNXS1fT9oWn77HiaV4702vVo3702 intact-crc:C48F1A451A03BE6F|rigid:PVjJPmk5HX7az6f+meEeekwZEuk false 35s radiolabel:?-? his tag:c-c|sufficient binding region:1-265|mutation decreasing interaction:130-130 - - psi-mi:"MI:0821"(molecular weight estimation by autoradiography) psi-mi:"MI:0821"(molecular weight estimation by autoradiography)