#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P11972	uniprotkb:P0CI39	intact:EBI-18232|uniprotkb:Q06207|uniprotkb:D6VZ86|ensemblfungi:YLR452C	intact:EBI-18360|uniprotkb:Q70D63|uniprotkb:Q70D65|uniprotkb:Q70D69|uniprotkb:Q70D73|uniprotkb:Q70D74|uniprotkb:P06842	psi-mi:sst2_yeast(display_long)|uniprotkb:SST2(gene name)|psi-mi:SST2(display_short)|uniprotkb:L9324.9(orf name)|uniprotkb:YLR452C(locus name)	psi-mi:ste2_yeasx(display_long)|uniprotkb:STE2(gene name)|psi-mi:STE2(display_short)	psi-mi:"MI:0096"(pull down)	Ballon et al. (2006)	imex:IM-12038|pubmed:16990133	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-1099517|imex:IM-12038-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_013557.1|sgd:S000004444|ensemblfungi:YLR452C(gene)|ensemblfungi:YLR452C_mRNA(transcript)|go:"GO:0000754"(adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0035556"(intracellular signal transduction)|interpro:IPR000591(Pleckstrin/ G-protein, interacting region)|interpro:IPR036305|interpro:IPR036388|interpro:IPR036390|interpro:IPR016137(Regulator of G protein signalling superfamily)|dip:DIP-2354N|mint:P11972	interpro:IPR000366(Fungal pheromone mating factor STE2 GPCR)|go:"GO:0004932"(mating-type factor pheromone receptor activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019236"(response to pheromone)|interpro:IPR027458|mint:P0CI39	go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)	-	-	figure legend:4 A|comment:No detectable  Gpa1 was present in the pull-down, as judged by immunoblotting  with an anti-Gpa1 antibody.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2006/09/26	2014/10/16	rogid:a452jzptv3MtejNfc+LDrwSFSks559292	rogid:Xw+4tatIKPl42hhE5XQUighaCP44932	intact-crc:3B52097BA9BD20FB|rigid:uD9Uljl2bDm22EbQspNth/MI/0I	false	myc tag:?-?|mutation disrupting interaction:304-304	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P11972	uniprotkb:P0CI39	intact:EBI-18232|uniprotkb:Q06207|uniprotkb:D6VZ86|ensemblfungi:YLR452C	intact:EBI-18360|uniprotkb:Q70D63|uniprotkb:Q70D65|uniprotkb:Q70D69|uniprotkb:Q70D73|uniprotkb:Q70D74|uniprotkb:P06842	psi-mi:sst2_yeast(display_long)|uniprotkb:SST2(gene name)|psi-mi:SST2(display_short)|uniprotkb:L9324.9(orf name)|uniprotkb:YLR452C(locus name)	psi-mi:ste2_yeasx(display_long)|uniprotkb:STE2(gene name)|psi-mi:STE2(display_short)	psi-mi:"MI:0096"(pull down)	Ballon et al. (2006)	imex:IM-12038|pubmed:16990133	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-1099535|imex:IM-12038-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_013557.1|sgd:S000004444|ensemblfungi:YLR452C(gene)|ensemblfungi:YLR452C_mRNA(transcript)|go:"GO:0000754"(adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0035556"(intracellular signal transduction)|interpro:IPR000591(Pleckstrin/ G-protein, interacting region)|interpro:IPR036305|interpro:IPR036388|interpro:IPR036390|interpro:IPR016137(Regulator of G protein signalling superfamily)|dip:DIP-2354N|mint:P11972	interpro:IPR000366(Fungal pheromone mating factor STE2 GPCR)|go:"GO:0004932"(mating-type factor pheromone receptor activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019236"(response to pheromone)|interpro:IPR027458|mint:P0CI39	go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)	-	-	figure legend:4 B|comment:The Sst2 protein was expressed in insect cells.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2006/09/26	2014/10/16	rogid:a452jzptv3MtejNfc+LDrwSFSks559292	rogid:Xw+4tatIKPl42hhE5XQUighaCP44932	intact-crc:9A0A0ED4E8497A73|rigid:uD9Uljl2bDm22EbQspNth/MI/0I	false	his tag:?-?	flag tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P11972	uniprotkb:P0CI39	intact:EBI-18232|uniprotkb:Q06207|uniprotkb:D6VZ86|ensemblfungi:YLR452C	intact:EBI-18360|uniprotkb:Q70D63|uniprotkb:Q70D65|uniprotkb:Q70D69|uniprotkb:Q70D73|uniprotkb:Q70D74|uniprotkb:P06842	psi-mi:sst2_yeast(display_long)|uniprotkb:SST2(gene name)|psi-mi:SST2(display_short)|uniprotkb:L9324.9(orf name)|uniprotkb:YLR452C(locus name)	psi-mi:ste2_yeasx(display_long)|uniprotkb:STE2(gene name)|psi-mi:STE2(display_short)	psi-mi:"MI:0018"(two hybrid)	Ballon et al. (2006)	imex:IM-12038|pubmed:16990133	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-1099822|imex:IM-12038-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_013557.1|sgd:S000004444|ensemblfungi:YLR452C(gene)|ensemblfungi:YLR452C_mRNA(transcript)|go:"GO:0000754"(adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0035556"(intracellular signal transduction)|interpro:IPR000591(Pleckstrin/ G-protein, interacting region)|interpro:IPR036305|interpro:IPR036388|interpro:IPR036390|interpro:IPR016137(Regulator of G protein signalling superfamily)|dip:DIP-2354N|mint:P11972	interpro:IPR000366(Fungal pheromone mating factor STE2 GPCR)|go:"GO:0004932"(mating-type factor pheromone receptor activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019236"(response to pheromone)|interpro:IPR027458|mint:P0CI39	go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)	-	-	figure legend:4 C, D|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2006/09/26	2014/10/16	rogid:a452jzptv3MtejNfc+LDrwSFSks559292	rogid:Xw+4tatIKPl42hhE5XQUighaCP44932	intact-crc:714F3A2625DD2941|rigid:uD9Uljl2bDm22EbQspNth/MI/0I	false	fusion protein:?-?|mutation disrupting interaction:304-304|mutation disrupting interaction:304-304|mutation disrupting interaction:304-304	fusion protein:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0CI39	uniprotkb:P11972	intact:EBI-18360|uniprotkb:Q70D63|uniprotkb:Q70D65|uniprotkb:Q70D69|uniprotkb:Q70D73|uniprotkb:Q70D74|uniprotkb:P06842	intact:EBI-18232|uniprotkb:Q06207|uniprotkb:D6VZ86|ensemblfungi:YLR452C	psi-mi:ste2_yeasx(display_long)|uniprotkb:STE2(gene name)|psi-mi:STE2(display_short)	psi-mi:sst2_yeast(display_long)|uniprotkb:SST2(gene name)|psi-mi:SST2(display_short)|uniprotkb:L9324.9(orf name)|uniprotkb:YLR452C(locus name)	psi-mi:"MI:0663"(confocal microscopy)	Ballon et al. (2006)	imex:IM-12038|pubmed:16990133	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0469"(IntAct)	intact:EBI-1099849|imex:IM-12038-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR000366(Fungal pheromone mating factor STE2 GPCR)|go:"GO:0004932"(mating-type factor pheromone receptor activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019236"(response to pheromone)|interpro:IPR027458|mint:P0CI39	refseq:NP_013557.1|sgd:S000004444|ensemblfungi:YLR452C(gene)|ensemblfungi:YLR452C_mRNA(transcript)|go:"GO:0000754"(adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0035556"(intracellular signal transduction)|interpro:IPR000591(Pleckstrin/ G-protein, interacting region)|interpro:IPR036305|interpro:IPR036388|interpro:IPR036390|interpro:IPR016137(Regulator of G protein signalling superfamily)|dip:DIP-2354N|mint:P11972	go:"GO:0005937"(mating projection)|go:"GO:0005886"(plasma membrane)|go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)	-	-	figure legend:5 B, C, 6 C, S3|comment:The colocalisation of SSt2 with Ste2 requires the function of the Sst2 DEP domain.|caution:The authors do not give the position of the 7 lysines in the cytoplasmic tail of Sste2 that were mutated to prevent endocytosis. The positions given refer to the 7 most C-terminal lysines found in the UniprotKb sequence.|comment:"This interaction took place in an exponentially growing culture of a Gpa1(gly302ser) mutant."|comment:"In naive cells the proteins colocalise all around the perimeter of the cell. However, by the time cells form prominent shmoos after exposure to pheromone, Sst2-GFP concentrates at the tip of the shmoo and does not associate with Ste2(7K-to-R)-mCherry elsewhere in the plasma membrane."|comment:"In cells exposed to pheromone and expressing Ste2(7K-to-R, 19S&T-to-A)-mCherry there was colocalisation in every cells; whereas in cells expressing Ste2(7K-to-R)-mCherry colocalisation only occurs at the shmoo tip."|comment:"In cells exposed to pheromone, where Yck1- and Yck2-mediated phosphorylation was ablated by mutations, Sst2-GFP associated with Ste2(7K-toR)around the whole perimeter of the cell."|comment:"The Ste2(7K-to-R, 19S/R-to-A) construct carried all 7 lys-to-arg mutations listed above and all 19 ser&thr to ala listed above."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2006/09/26	2014/10/16	rogid:Xw+4tatIKPl42hhE5XQUighaCP44932	rogid:a452jzptv3MtejNfc+LDrwSFSks559292	intact-crc:2392E88C91B3C53A|rigid:uD9Uljl2bDm22EbQspNth/MI/0I	false	binding-associated region:297-431|fluorescent protein tag:c-c|mutation increasing interaction:422-422,400-400,387-387,374-374,358-358,352-352,337-337|mutation increasing interaction:331-331,337-337,352-352,358-358,374-374,387-387,400-400,422-422,338-338,363-363,354-354,385-385,356-356,339-339,368-368,355-355,386-386,398-398,366-366,382-382,384-384,389-389,411-411	mutation disrupting interaction:304-304|green fluorescent protein tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
intact:EBI-1099879	uniprotkb:P11972	-	intact:EBI-18232|uniprotkb:Q06207|uniprotkb:D6VZ86|ensemblfungi:YLR452C	psi-mi:gpa1-ste2_protein(display_short)|psi-mi:EBI-1099879(display_long)	psi-mi:sst2_yeast(display_long)|uniprotkb:SST2(gene name)|psi-mi:SST2(display_short)|uniprotkb:L9324.9(orf name)|uniprotkb:YLR452C(locus name)	psi-mi:"MI:0663"(confocal microscopy)	Ballon et al. (2006)	imex:IM-12038|pubmed:16990133	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0469"(IntAct)	intact:EBI-1099889|imex:IM-12038-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	uniprotkb:P08539(multiple parent)|uniprotkb:P06842(multiple parent)	refseq:NP_013557.1|sgd:S000004444|ensemblfungi:YLR452C(gene)|ensemblfungi:YLR452C_mRNA(transcript)|go:"GO:0000754"(adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0035556"(intracellular signal transduction)|interpro:IPR000591(Pleckstrin/ G-protein, interacting region)|interpro:IPR036305|interpro:IPR036388|interpro:IPR036390|interpro:IPR016137(Regulator of G protein signalling superfamily)|dip:DIP-2354N|mint:P11972	go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)|go:"GO:0005886"(plasma membrane)|go:"GO:0005933"(cellular bud)	no-uniprot-update:An amino acid sequence which is not represented in UniProt	-	figure legend:6 B|comment:"The Ste2(297-431) fusion protein recruited Sst2 to the cell membrane, especially in the bud."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2006/09/26	2014/10/16	rogid:SrWYCG+GxVkMEhsCgHTCEG7zf3s4932	rogid:a452jzptv3MtejNfc+LDrwSFSks559292	intact-crc:0820EF4451EFFEE5|rigid:sXIuOp0of/rfISxxmjljmCpOBJA	false	fluorescent protein tag:c-c	mutation disrupting interaction:304-304|green fluorescent protein tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P06783	uniprotkb:P11972	intact:EBI-18366|uniprotkb:D6VX23|ensemblfungi:YKL178C	intact:EBI-18232|uniprotkb:Q06207|uniprotkb:D6VZ86|ensemblfungi:YLR452C	psi-mi:ste3_yeast(display_long)|uniprotkb:STE3(gene name)|psi-mi:STE3(display_short)|uniprotkb:YKL178C(locus name)	psi-mi:sst2_yeast(display_long)|uniprotkb:SST2(gene name)|psi-mi:SST2(display_short)|uniprotkb:L9324.9(orf name)|uniprotkb:YLR452C(locus name)	psi-mi:"MI:0663"(confocal microscopy)	Ballon et al. (2006)	imex:IM-12038|pubmed:16990133	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0469"(IntAct)	intact:EBI-1099905|imex:IM-12038-6	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_012743.1|sgd:S000001661|ensemblfungi:YKL178C(gene)|ensemblfungi:YKL178C_mRNA(transcript)|go:"GO:0000750"(pheromone-dependent signal transduction involved in conjugation with cellular fusion)|go:"GO:0000755"(cytogamy)|go:"GO:0004932"(mating-type factor pheromone receptor activity)|go:"GO:0004933"(mating-type a-factor pheromone receptor activity)|go:"GO:0005775"(vacuolar lumen)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005887"(integral component of plasma membrane)|interpro:IPR001499(Fungal pheromone STE3 GPCR)|dip:DIP-1146N|mint:P06783	refseq:NP_013557.1|sgd:S000004444|ensemblfungi:YLR452C(gene)|ensemblfungi:YLR452C_mRNA(transcript)|go:"GO:0000754"(adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0035556"(intracellular signal transduction)|interpro:IPR000591(Pleckstrin/ G-protein, interacting region)|interpro:IPR036305|interpro:IPR036388|interpro:IPR036390|interpro:IPR016137(Regulator of G protein signalling superfamily)|dip:DIP-2354N|mint:P11972	go:"GO:0008277"(regulation of G protein-coupled receptor signaling pathway)|go:"GO:0005886"(plasma membrane)	crc64:5DBF611076B8CA91	-	figure legend:S4|caution:The authors do not give the residue position of the 3 mutated lysines but the positions given are the 3 most c-terminal lysines.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2006/09/26	2014/10/16	rogid:F7LD1XL+DteOP3zzR5mbiCMBgpA559292	rogid:a452jzptv3MtejNfc+LDrwSFSks559292	intact-crc:71E4FFE917539397|rigid:eCjWZQ0DMAyCWLlSPn75FGnqHQ4	false	fluorescent protein tag:c-c|mutation increasing interaction:453-453,432-432,424-424	green fluorescent protein tag:?-?|mutation disrupting interaction:304-304	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)