#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P84092	uniprotkb:P19814	intact:EBI-297693|uniprotkb:P53679|uniprotkb:P20172|ensembl:ENSRNOP00000070202	intact:EBI-541446|uniprotkb:Q4G0B6|ensembl:ENSRNOP00000019713	psi-mi:ap2m1_rat(display_long)|uniprotkb:Adaptor protein complex AP-2 subunit mu(gene name synonym)|uniprotkb:AP-2 mu chain(gene name synonym)|uniprotkb:Ap2m1(gene name)|psi-mi:Ap2m1(display_short)|uniprotkb:Mu2-adaptin(gene name synonym)|uniprotkb:Plasma membrane adaptor AP-2 50 kDa protein(gene name synonym)|uniprotkb:Clathrin coat assembly protein AP50(gene name synonym)|uniprotkb:Clathrin coat-associated protein AP50(gene name synonym)|uniprotkb:Clathrin assembly protein complex 2 mu medium chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 2 subunit mu(gene name synonym)	psi-mi:tgon3_rat(display_long)|uniprotkb:Ttgn1(gene name)|psi-mi:Ttgn1(display_short)	psi-mi:"MI:0018"(two hybrid)	Stephens et al. (1997)	pubmed:9162036	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-590991	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0098794"(postsynapse)|go:"GO:0098884"(postsynaptic neurotransmitter receptor internalization)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1900244"(positive regulation of synaptic vesicle endocytosis)|go:"GO:1903077"(negative regulation of protein localization to plasma membrane)|interpro:IPR001392(Clathrin adaptor, mu subunit)|interpro:IPR011012(Longin-like)|interpro:IPR018240|interpro:IPR022775|interpro:IPR028565|interpro:IPR036168|interpro:IPR043512|interpro:IPR043532|mint:P84092|rcsb pdb:1BW8|rcsb pdb:1BXX|rcsb pdb:1HES|rcsb pdb:1I31|rcsb pdb:2BP5|rcsb pdb:2JKR|rcsb pdb:2JKT|rcsb pdb:2PR9|rcsb pdb:2VGL|rcsb pdb:2XA7|rcsb pdb:3H85|rcsb pdb:3ML6|rcsb pdb:4UQI|rcsb pdb:5C7Z|rcsb pdb:5FPI|rcsb pdb:5WRK|go:"GO:0097718"(disordered domain specific binding)|rcsb pdb:5WRL|rcsb pdb:5WRM|rcsb pdb:6QH5|rcsb pdb:6QH6|rcsb pdb:6RH6|rcsb pdb:6YAE|rcsb pdb:6YAF|rcsb pdb:6YAH|reactome:R-RNO-177504|reactome:R-RNO-190873|reactome:R-RNO-196025|reactome:R-RNO-2132295|reactome:R-RNO-416993|reactome:R-RNO-437239|reactome:R-RNO-5099900|reactome:R-RNO-5140745|reactome:R-RNO-8856825|reactome:R-RNO-8856828|reactome:R-RNO-8866427|reactome:R-RNO-8964038|ensembl:ENSRNOG00000001709(gene)|ensembl:ENSRNOT00000088821(transcript)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0005048"(signal sequence binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0008289"(lipid binding)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0030122"(AP-2 adaptor complex)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031623"(receptor internalization)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0043195"(terminal bouton)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0044325"(transmembrane transporter binding)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0050750"(low-density lipoprotein particle receptor binding)|go:"GO:0065003"(protein-containing complex assembly)|go:"GO:0072583"(clathrin-dependent endocytosis)|go:"GO:0097494"(regulation of vesicle size)|rgd:620135|refseq:NP_446289.1|dip:DIP-29761N	ensembl:ENSRNOG00000014617(gene)|ensembl:ENSRNOT00000019713(transcript)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006895"(Golgi to endosome transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030140"(trans-Golgi network transport vesicle)|mint:P19814|rcsb pdb:4IKN|reactome:R-RNO-381426|reactome:R-RNO-432722|reactome:R-RNO-6811440|reactome:R-RNO-8856825|reactome:R-RNO-8856828|reactome:R-RNO-8957275|rgd:620445|refseq:NP_620195.1	-	-	-	comment:"The  tyrosine 350 and Serine 348 hotspotsare in the transport signal motif SDYQRL (348-353)"	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2005/05/25	2014/10/16	rogid:AyJEJTqzHtIRWEnMGBzLQP3H5lA10116	rogid:4CCi78ObiE8wUUkGZP0AF4geI1I10116	intact-crc:03E13EB9850B0584|rigid:cAziV2vw+c1VY5F9tCqsfgbI0/o	false	-	mutation decreasing interaction:350-350|binding-associated region:349-354|mutation decreasing interaction:348-348	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P84092	uniprotkb:P19814	intact:EBI-297693|uniprotkb:P53679|uniprotkb:P20172|ensembl:ENSRNOP00000070202	intact:EBI-541446|uniprotkb:Q4G0B6|ensembl:ENSRNOP00000019713	psi-mi:ap2m1_rat(display_long)|uniprotkb:Adaptor protein complex AP-2 subunit mu(gene name synonym)|uniprotkb:AP-2 mu chain(gene name synonym)|uniprotkb:Ap2m1(gene name)|psi-mi:Ap2m1(display_short)|uniprotkb:Mu2-adaptin(gene name synonym)|uniprotkb:Plasma membrane adaptor AP-2 50 kDa protein(gene name synonym)|uniprotkb:Clathrin coat assembly protein AP50(gene name synonym)|uniprotkb:Clathrin coat-associated protein AP50(gene name synonym)|uniprotkb:Clathrin assembly protein complex 2 mu medium chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 2 subunit mu(gene name synonym)	psi-mi:tgon3_rat(display_long)|uniprotkb:Ttgn1(gene name)|psi-mi:Ttgn1(display_short)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Stephens et al. (1997)	pubmed:9162036	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-591010	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0098794"(postsynapse)|go:"GO:0098884"(postsynaptic neurotransmitter receptor internalization)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1900244"(positive regulation of synaptic vesicle endocytosis)|go:"GO:1903077"(negative regulation of protein localization to plasma membrane)|interpro:IPR001392(Clathrin adaptor, mu subunit)|interpro:IPR011012(Longin-like)|interpro:IPR018240|interpro:IPR022775|interpro:IPR028565|interpro:IPR036168|interpro:IPR043512|interpro:IPR043532|mint:P84092|rcsb pdb:1BW8|rcsb pdb:1BXX|rcsb pdb:1HES|rcsb pdb:1I31|rcsb pdb:2BP5|rcsb pdb:2JKR|rcsb pdb:2JKT|rcsb pdb:2PR9|rcsb pdb:2VGL|rcsb pdb:2XA7|rcsb pdb:3H85|rcsb pdb:3ML6|rcsb pdb:4UQI|rcsb pdb:5C7Z|rcsb pdb:5FPI|rcsb pdb:5WRK|go:"GO:0097718"(disordered domain specific binding)|rcsb pdb:5WRL|rcsb pdb:5WRM|rcsb pdb:6QH5|rcsb pdb:6QH6|rcsb pdb:6RH6|rcsb pdb:6YAE|rcsb pdb:6YAF|rcsb pdb:6YAH|reactome:R-RNO-177504|reactome:R-RNO-190873|reactome:R-RNO-196025|reactome:R-RNO-2132295|reactome:R-RNO-416993|reactome:R-RNO-437239|reactome:R-RNO-5099900|reactome:R-RNO-5140745|reactome:R-RNO-8856825|reactome:R-RNO-8856828|reactome:R-RNO-8866427|reactome:R-RNO-8964038|ensembl:ENSRNOG00000001709(gene)|ensembl:ENSRNOT00000088821(transcript)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0005048"(signal sequence binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0008289"(lipid binding)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0030122"(AP-2 adaptor complex)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031623"(receptor internalization)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0043195"(terminal bouton)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0044325"(transmembrane transporter binding)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0050750"(low-density lipoprotein particle receptor binding)|go:"GO:0065003"(protein-containing complex assembly)|go:"GO:0072583"(clathrin-dependent endocytosis)|go:"GO:0097494"(regulation of vesicle size)|rgd:620135|refseq:NP_446289.1|dip:DIP-29761N	ensembl:ENSRNOG00000014617(gene)|ensembl:ENSRNOT00000019713(transcript)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006895"(Golgi to endosome transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030140"(trans-Golgi network transport vesicle)|mint:P19814|rcsb pdb:4IKN|reactome:R-RNO-381426|reactome:R-RNO-432722|reactome:R-RNO-6811440|reactome:R-RNO-8856825|reactome:R-RNO-8856828|reactome:R-RNO-8957275|rgd:620445|refseq:NP_620195.1	-	-	-	comment:"The  tyrosine 350 and Serine 348 hotspots are in the transport signal motif SDYQRL (348-353)"|comment:Both bait and prey are purified.	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2005/05/25	2014/10/16	rogid:AyJEJTqzHtIRWEnMGBzLQP3H5lA10116	rogid:4CCi78ObiE8wUUkGZP0AF4geI1I10116	intact-crc:3C9ACD2BED18F463|rigid:cAziV2vw+c1VY5F9tCqsfgbI0/o	false	binding-associated region:121-435|tag:?-?	mutation decreasing interaction:350-350|binding-associated region:349-354|mutation decreasing interaction:348-348|glutathione s tranferase tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P19814	uniprotkb:P84092	intact:EBI-541446|uniprotkb:Q4G0B6|ensembl:ENSRNOP00000019713	intact:EBI-297693|uniprotkb:P53679|uniprotkb:P20172|ensembl:ENSRNOP00000070202	psi-mi:tgon3_rat(display_long)|uniprotkb:Ttgn1(gene name)|psi-mi:Ttgn1(display_short)	psi-mi:ap2m1_rat(display_long)|uniprotkb:Adaptor protein complex AP-2 subunit mu(gene name synonym)|uniprotkb:AP-2 mu chain(gene name synonym)|uniprotkb:Ap2m1(gene name)|psi-mi:Ap2m1(display_short)|uniprotkb:Mu2-adaptin(gene name synonym)|uniprotkb:Plasma membrane adaptor AP-2 50 kDa protein(gene name synonym)|uniprotkb:Clathrin coat assembly protein AP50(gene name synonym)|uniprotkb:Clathrin coat-associated protein AP50(gene name synonym)|uniprotkb:Clathrin assembly protein complex 2 mu medium chain(gene name synonym)|uniprotkb:Adaptor-related protein complex 2 subunit mu(gene name synonym)	psi-mi:"MI:0017"(classical fluorescence spectroscopy)	Stephens et al. (1997)	pubmed:9162036	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-591030	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSRNOG00000014617(gene)|ensembl:ENSRNOT00000019713(transcript)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006895"(Golgi to endosome transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030140"(trans-Golgi network transport vesicle)|mint:P19814|rcsb pdb:4IKN|reactome:R-RNO-381426|reactome:R-RNO-432722|reactome:R-RNO-6811440|reactome:R-RNO-8856825|reactome:R-RNO-8856828|reactome:R-RNO-8957275|rgd:620445|refseq:NP_620195.1	go:"GO:0098794"(postsynapse)|go:"GO:0098884"(postsynaptic neurotransmitter receptor internalization)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1900244"(positive regulation of synaptic vesicle endocytosis)|go:"GO:1903077"(negative regulation of protein localization to plasma membrane)|interpro:IPR001392(Clathrin adaptor, mu subunit)|interpro:IPR011012(Longin-like)|interpro:IPR018240|interpro:IPR022775|interpro:IPR028565|interpro:IPR036168|interpro:IPR043512|interpro:IPR043532|mint:P84092|rcsb pdb:1BW8|rcsb pdb:1BXX|rcsb pdb:1HES|rcsb pdb:1I31|rcsb pdb:2BP5|rcsb pdb:2JKR|rcsb pdb:2JKT|rcsb pdb:2PR9|rcsb pdb:2VGL|rcsb pdb:2XA7|rcsb pdb:3H85|rcsb pdb:3ML6|rcsb pdb:4UQI|rcsb pdb:5C7Z|rcsb pdb:5FPI|rcsb pdb:5WRK|go:"GO:0097718"(disordered domain specific binding)|rcsb pdb:5WRL|rcsb pdb:5WRM|rcsb pdb:6QH5|rcsb pdb:6QH6|rcsb pdb:6RH6|rcsb pdb:6YAE|rcsb pdb:6YAF|rcsb pdb:6YAH|reactome:R-RNO-177504|reactome:R-RNO-190873|reactome:R-RNO-196025|reactome:R-RNO-2132295|reactome:R-RNO-416993|reactome:R-RNO-437239|reactome:R-RNO-5099900|reactome:R-RNO-5140745|reactome:R-RNO-8856825|reactome:R-RNO-8856828|reactome:R-RNO-8866427|reactome:R-RNO-8964038|ensembl:ENSRNOG00000001709(gene)|ensembl:ENSRNOT00000088821(transcript)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0005048"(signal sequence binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0005905"(clathrin-coated pit)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0008289"(lipid binding)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0030122"(AP-2 adaptor complex)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031623"(receptor internalization)|go:"GO:0035615"(clathrin adaptor activity)|go:"GO:0043195"(terminal bouton)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0044325"(transmembrane transporter binding)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0050750"(low-density lipoprotein particle receptor binding)|go:"GO:0065003"(protein-containing complex assembly)|go:"GO:0072583"(clathrin-dependent endocytosis)|go:"GO:0097494"(regulation of vesicle size)|rgd:620135|refseq:NP_446289.1|dip:DIP-29761N	-	-	-	comment:"The  tyrosine 350 and Serine 348 hotspots are in the transport signal motif SDYQRL (348-353)"|comment:Both bait and prey are purified.|kinetics:The affinity between TGN38 cytoplasmic tail and the mutant Y350A adaptin mu2 mutant is kd = 7.55 10-7 M|kinetics:The affinity between TGN38 cytoplasmic tail and the mutant S348A adaptin mu2 mutant is kd = 1.03 10-7 M	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2005/05/25	2014/10/16	rogid:4CCi78ObiE8wUUkGZP0AF4geI1I10116	rogid:AyJEJTqzHtIRWEnMGBzLQP3H5lA10116	intact-crc:3CB8D0CB32C9FA7D|rigid:cAziV2vw+c1VY5F9tCqsfgbI0/o	false	binding-associated region:349-354|mutation decreasing interaction:348-348|glutathione s tranferase tag:?-?|mutation decreasing interaction:350-350	binding-associated region:121-435|tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)