#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q9QWY8	uniprotkb:P84080	intact:EBI-698498|uniprotkb:Q99LV8|uniprotkb:Q9Z2B6|uniprotkb:O08612|uniprotkb:Q80TG8|uniprotkb:Q80UV6|uniprotkb:Q505F0|ensembl:ENSMUSP00000134825	intact:EBI-449051|uniprotkb:A5D7F1|uniprotkb:P10947|uniprotkb:P32889|uniprotkb:Q3SZG1|ensembl:ENSBTAP00000010159	psi-mi:asap1_mouse(display_long)|uniprotkb:PIP2-dependent ARF1 GAP(gene name synonym)|uniprotkb:ADP-ribosylation factor-directed GTPase-activating protein 1(gene name synonym)|uniprotkb:Development and differentiation-enhancing factor 1(gene name synonym)|uniprotkb:Asap1(gene name)|psi-mi:Asap1(display_short)|uniprotkb:Ddef1(gene name synonym)|uniprotkb:Kiaa1249(gene name synonym)|uniprotkb:Shag1(gene name synonym)|uniprotkb:130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein(gene name synonym)	psi-mi:arf1_bovin(display_long)|uniprotkb:ARF1(gene name)|psi-mi:ARF1(display_short)	psi-mi:"MI:0027"(cosedimentation)	Klein et al. (2005)	pubmed:16223492|mint:MINT-5218002|imex:IM-11058	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-698541|imex:IM-11058-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001263396.1|refseq:NP_034156.2|refseq:NP_001263390.1|refseq:NP_001263391.1|ensembl:ENSMUSG00000022377(gene)|ensembl:ENSMUST00000177374(transcript)|go:"GO:0001786"(phosphatidylserine binding)|go:"GO:0002102"(podosome)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005546"(phosphatidylinositol-4,5-bisphosphate binding)|go:"GO:0005547"(phosphatidylinositol-3,4,5-trisphosphate binding)|go:"GO:0005829"(cytosol)|go:"GO:0031253"(cell projection membrane)|go:"GO:0043197"(dendritic spine)|go:"GO:0043547"(positive regulation of GTPase activity)|go:"GO:0046872"(metal ion binding)|go:"GO:0060271"(cilium assembly)|go:"GO:0061000"(negative regulation of dendritic spine development)|go:"GO:0071803"(positive regulation of podosome assembly)|go:"GO:1903527"(positive regulation of membrane tubulation)|interpro:IPR001164(Arf GTPase activating protein)|interpro:IPR001452(Src homology-3)|interpro:IPR001849(Pleckstrin-like)|interpro:IPR002110(Ankyrin)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR020683|interpro:IPR027267|interpro:IPR036028|interpro:IPR036770|interpro:IPR037278|interpro:IPR037844|interpro:IPR037928|interpro:IPR038016|interpro:IPR038508|interpro:IPR043593|mint:Q9QWY8|rcsb pdb:5C6R|rcsb pdb:5C79|reactome:R-MMU-5620916	refseq:NP_788826.1|ensembl:ENSBTAG00000007725(gene)|ensembl:ENSBTAT00000010159(transcript)|go:"GO:0002090"(regulation of receptor internalization)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0034315"(regulation of Arp2/3 complex-mediated actin nucleation)|go:"GO:0043005"(neuron projection)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0060292"(long-term synaptic depression)|go:"GO:0097061"(dendritic spine organization)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR006689(ARF/SAR superfamily)|interpro:IPR027417|rcsb pdb:1R8Q|rcsb pdb:1R8S|rcsb pdb:1S9D|rcsb pdb:4C0A|reactome:R-BTA-199992|reactome:R-BTA-6807878|reactome:R-BTA-6811434|reactome:R-BTA-6811438|refseq:XP_005208456.1|refseq:XP_005208457.1	-	-	-	comment:"liposome sedimentation: only 27% of myr-Arf1 GDP and 34% of ASAP1 PZA fragment were recovered in the pelleted membrane fraction if alone. The addition of AlFx did not change the sedimentation properties of each proteins. When mixed together in presence of AlFx, both PZA and and myr-Arf1 translocated to membrane fraction within the same proportion 74% and 83% respectively."|stimulant:"AlFx (Aluminum fluoride) stabilize the complex formation between Arf1-GDP and PZA fragment of ASAP1 by mimicking activated form of Arf1, Arf1-GDP-AlFx."|comment:Both proteins are purified to homogeneity. Myristoylated Arf1 was produced by co-expressing in E. coli the yeast N-myristoyl-transferase.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2005/10/17	2014/10/16	rogid:/jhMt+/t+DTqI3ZQmzU0IMFCHdA10090	rogid:zray9asm6VonxpuJpv0dRBspPt89913	intact-crc:BCA52B218FE19787|rigid:7Xbogy8/qI7eThUt82jCF3U10nc	false	sufficient binding region:325-724|his tag:n-n	N-myristoylglycine:2-2	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q9QWY8	uniprotkb:P84080	intact:EBI-698498|uniprotkb:Q99LV8|uniprotkb:Q9Z2B6|uniprotkb:O08612|uniprotkb:Q80TG8|uniprotkb:Q80UV6|uniprotkb:Q505F0|ensembl:ENSMUSP00000134825	intact:EBI-449051|uniprotkb:A5D7F1|uniprotkb:P10947|uniprotkb:P32889|uniprotkb:Q3SZG1|ensembl:ENSBTAP00000010159	psi-mi:asap1_mouse(display_long)|uniprotkb:PIP2-dependent ARF1 GAP(gene name synonym)|uniprotkb:ADP-ribosylation factor-directed GTPase-activating protein 1(gene name synonym)|uniprotkb:Development and differentiation-enhancing factor 1(gene name synonym)|uniprotkb:Asap1(gene name)|psi-mi:Asap1(display_short)|uniprotkb:Ddef1(gene name synonym)|uniprotkb:Kiaa1249(gene name synonym)|uniprotkb:Shag1(gene name synonym)|uniprotkb:130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein(gene name synonym)	psi-mi:arf1_bovin(display_long)|uniprotkb:ARF1(gene name)|psi-mi:ARF1(display_short)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Klein et al. (2005)	pubmed:16223492|mint:MINT-5218002|imex:IM-11058	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-698561|imex:IM-11058-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001263396.1|refseq:NP_034156.2|refseq:NP_001263390.1|refseq:NP_001263391.1|ensembl:ENSMUSG00000022377(gene)|ensembl:ENSMUST00000177374(transcript)|go:"GO:0001786"(phosphatidylserine binding)|go:"GO:0002102"(podosome)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005546"(phosphatidylinositol-4,5-bisphosphate binding)|go:"GO:0005547"(phosphatidylinositol-3,4,5-trisphosphate binding)|go:"GO:0005829"(cytosol)|go:"GO:0031253"(cell projection membrane)|go:"GO:0043197"(dendritic spine)|go:"GO:0043547"(positive regulation of GTPase activity)|go:"GO:0046872"(metal ion binding)|go:"GO:0060271"(cilium assembly)|go:"GO:0061000"(negative regulation of dendritic spine development)|go:"GO:0071803"(positive regulation of podosome assembly)|go:"GO:1903527"(positive regulation of membrane tubulation)|interpro:IPR001164(Arf GTPase activating protein)|interpro:IPR001452(Src homology-3)|interpro:IPR001849(Pleckstrin-like)|interpro:IPR002110(Ankyrin)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR020683|interpro:IPR027267|interpro:IPR036028|interpro:IPR036770|interpro:IPR037278|interpro:IPR037844|interpro:IPR037928|interpro:IPR038016|interpro:IPR038508|interpro:IPR043593|mint:Q9QWY8|rcsb pdb:5C6R|rcsb pdb:5C79|reactome:R-MMU-5620916	refseq:NP_788826.1|ensembl:ENSBTAG00000007725(gene)|ensembl:ENSBTAT00000010159(transcript)|go:"GO:0002090"(regulation of receptor internalization)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0034315"(regulation of Arp2/3 complex-mediated actin nucleation)|go:"GO:0043005"(neuron projection)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0060292"(long-term synaptic depression)|go:"GO:0097061"(dendritic spine organization)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR006689(ARF/SAR superfamily)|interpro:IPR027417|rcsb pdb:1R8Q|rcsb pdb:1R8S|rcsb pdb:1S9D|rcsb pdb:4C0A|reactome:R-BTA-199992|reactome:R-BTA-6807878|reactome:R-BTA-6811434|reactome:R-BTA-6811438|refseq:XP_005208456.1|refseq:XP_005208457.1	-	-	-	stimulant:"AlFx (Aluminum fluoride) stabilize the complex formation between Arf1-GDP and ASAP1 by mimicking activated form of Arf1, Arf1-GDP-AlFx."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:36483(crisp-kidney)|taxid:36483(Cricetidae sp.)	-	2005/10/17	2014/10/16	rogid:/jhMt+/t+DTqI3ZQmzU0IMFCHdA10090	rogid:zray9asm6VonxpuJpv0dRBspPt89913	intact-crc:BA5B1AC6BB3EF71D|rigid:7Xbogy8/qI7eThUt82jCF3U10nc	false	flag tag:n-n	ha tag:n-n	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)