#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P25369 uniprotkb:P32790 intact:EBI-10218|uniprotkb:P87004|uniprotkb:Q8NIM9|uniprotkb:D6VQY1|ensemblfungi:YCL034W intact:EBI-17313|uniprotkb:D6VPZ3|ensemblfungi:YBL007C psi-mi:lsb5_yeast(display_long)|uniprotkb:YCL186(orf name)|uniprotkb:YCL34W(orf name)|uniprotkb:YCL034W(locus name)|uniprotkb:LSB5(gene name)|psi-mi:LSB5(display_short) psi-mi:sla1_yeast(display_long)|uniprotkb:YBL0321(orf name)|uniprotkb:YBL007C(locus name)|uniprotkb:SLA1(gene name)|psi-mi:SLA1(display_short) psi-mi:"MI:0018"(two hybrid) Costa et al. (2005) pubmed:15651983 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-476407 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_009896.2|sgd:S000000539|ensemblfungi:YCL034W(gene)|ensemblfungi:YCL034W_mRNA(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005938"(cell cortex)|go:"GO:0006897"(endocytosis)|go:"GO:0007015"(actin filament organization)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030479"(actin cortical patch)|go:"GO:0051666"(actin cortical patch localization)|interpro:IPR008942(ENTH/VHS)|interpro:IPR044103|dip:DIP-6258N refseq:NP_009546.1|sgd:S000000103|ensemblfungi:YBL007C(gene)|ensemblfungi:YBL007C_mRNA(transcript)|go:"GO:0000147"(actin cortical patch assembly)|go:"GO:0003779"(actin binding)|go:"GO:0005634"(nucleus)|go:"GO:0005886"(plasma membrane)|go:"GO:0005935"(cellular bud neck)|go:"GO:0005938"(cell cortex)|go:"GO:0006897"(endocytosis)|go:"GO:0010008"(endosome membrane)|go:"GO:0016197"(endosomal transport)|go:"GO:0030479"(actin cortical patch)|go:"GO:0034316"(negative regulation of Arp2/3 complex-mediated actin nucleation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043130"(ubiquitin binding)|go:"GO:0043332"(mating projection tip)|go:"GO:0044396"(actin cortical patch organization)|go:"GO:0140224"(SLAC complex)|go:"GO:0140312"(cargo adaptor activity)|go:"GO:1990964"(actin cytoskeleton-regulatory complex)|interpro:IPR001452(Src homology-3)|interpro:IPR007131(SLA1 homology domain 1, SHD1)|interpro:IPR013761(Sterile alpha motif-type)|interpro:IPR029922|interpro:IPR035800|interpro:IPR035821|interpro:IPR036028|rcsb pdb:1SSH|rcsb pdb:1Z9Z|rcsb pdb:2HBP|rcsb pdb:2JT4|rcsb pdb:2V1Q|rcsb pdb:3IDW|dip:DIP-695N|mint:P32790 - - crc64:7FD85AA776407624 - taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2005/01/21 2014/10/16 rogid:rOQErdx6EVNUSspb6WkbM6T02/I559292 rogid:uDuhC6oqoPZrlUhxWJmBGbozCAs559292 intact-crc:77A6C31640612444|rigid:R2pYtOBivV2Ov4JPloCpMBkW0cs false binding-associated region:174-354 binding-associated region:486-731(IPR007131) - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P32790 uniprotkb:P25369 intact:EBI-17313|uniprotkb:D6VPZ3|ensemblfungi:YBL007C intact:EBI-10218|uniprotkb:P87004|uniprotkb:Q8NIM9|uniprotkb:D6VQY1|ensemblfungi:YCL034W psi-mi:sla1_yeast(display_long)|uniprotkb:YBL0321(orf name)|uniprotkb:YBL007C(locus name)|uniprotkb:SLA1(gene name)|psi-mi:SLA1(display_short) psi-mi:lsb5_yeast(display_long)|uniprotkb:YCL186(orf name)|uniprotkb:YCL34W(orf name)|uniprotkb:YCL034W(locus name)|uniprotkb:LSB5(gene name)|psi-mi:LSB5(display_short) psi-mi:"MI:0018"(two hybrid) Costa et al. (2005) pubmed:15651983 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-476574 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_009546.1|sgd:S000000103|ensemblfungi:YBL007C(gene)|ensemblfungi:YBL007C_mRNA(transcript)|go:"GO:0000147"(actin cortical patch assembly)|go:"GO:0003779"(actin binding)|go:"GO:0005634"(nucleus)|go:"GO:0005886"(plasma membrane)|go:"GO:0005935"(cellular bud neck)|go:"GO:0005938"(cell cortex)|go:"GO:0006897"(endocytosis)|go:"GO:0010008"(endosome membrane)|go:"GO:0016197"(endosomal transport)|go:"GO:0030479"(actin cortical patch)|go:"GO:0034316"(negative regulation of Arp2/3 complex-mediated actin nucleation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043130"(ubiquitin binding)|go:"GO:0043332"(mating projection tip)|go:"GO:0044396"(actin cortical patch organization)|go:"GO:0140224"(SLAC complex)|go:"GO:0140312"(cargo adaptor activity)|go:"GO:1990964"(actin cytoskeleton-regulatory complex)|interpro:IPR001452(Src homology-3)|interpro:IPR007131(SLA1 homology domain 1, SHD1)|interpro:IPR013761(Sterile alpha motif-type)|interpro:IPR029922|interpro:IPR035800|interpro:IPR035821|interpro:IPR036028|rcsb pdb:1SSH|rcsb pdb:1Z9Z|rcsb pdb:2HBP|rcsb pdb:2JT4|rcsb pdb:2V1Q|rcsb pdb:3IDW|dip:DIP-695N|mint:P32790 refseq:NP_009896.2|sgd:S000000539|ensemblfungi:YCL034W(gene)|ensemblfungi:YCL034W_mRNA(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005938"(cell cortex)|go:"GO:0006897"(endocytosis)|go:"GO:0007015"(actin filament organization)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030479"(actin cortical patch)|go:"GO:0051666"(actin cortical patch localization)|interpro:IPR008942(ENTH/VHS)|interpro:IPR044103|dip:DIP-6258N - crc64:7FD85AA776407624 - comment:Fragment 301-354 interaction with HD1 domain of Sla1 is weaker than for fragment 174-354 containing a GAT domain.|comment:The lsb5 C-terminal NPF domain is essential for interaction with sla1. taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2005/01/21 2014/10/16 rogid:uDuhC6oqoPZrlUhxWJmBGbozCAs559292 rogid:rOQErdx6EVNUSspb6WkbM6T02/I559292 intact-crc:0ACF95885D89C207|rigid:R2pYtOBivV2Ov4JPloCpMBkW0cs false binding-associated region:486-555 binding-associated region:174-354|binding-associated region:174-345 - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P25369 uniprotkb:P32790 intact:EBI-10218|uniprotkb:P87004|uniprotkb:Q8NIM9|uniprotkb:D6VQY1|ensemblfungi:YCL034W intact:EBI-17313|uniprotkb:D6VPZ3|ensemblfungi:YBL007C psi-mi:lsb5_yeast(display_long)|uniprotkb:YCL186(orf name)|uniprotkb:YCL34W(orf name)|uniprotkb:YCL034W(locus name)|uniprotkb:LSB5(gene name)|psi-mi:LSB5(display_short) psi-mi:sla1_yeast(display_long)|uniprotkb:YBL0321(orf name)|uniprotkb:YBL007C(locus name)|uniprotkb:SLA1(gene name)|psi-mi:SLA1(display_short) psi-mi:"MI:0018"(two hybrid) Costa et al. (2005) pubmed:15651983 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-491440 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_009896.2|sgd:S000000539|ensemblfungi:YCL034W(gene)|ensemblfungi:YCL034W_mRNA(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005938"(cell cortex)|go:"GO:0006897"(endocytosis)|go:"GO:0007015"(actin filament organization)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030479"(actin cortical patch)|go:"GO:0051666"(actin cortical patch localization)|interpro:IPR008942(ENTH/VHS)|interpro:IPR044103|dip:DIP-6258N refseq:NP_009546.1|sgd:S000000103|ensemblfungi:YBL007C(gene)|ensemblfungi:YBL007C_mRNA(transcript)|go:"GO:0000147"(actin cortical patch assembly)|go:"GO:0003779"(actin binding)|go:"GO:0005634"(nucleus)|go:"GO:0005886"(plasma membrane)|go:"GO:0005935"(cellular bud neck)|go:"GO:0005938"(cell cortex)|go:"GO:0006897"(endocytosis)|go:"GO:0010008"(endosome membrane)|go:"GO:0016197"(endosomal transport)|go:"GO:0030479"(actin cortical patch)|go:"GO:0034316"(negative regulation of Arp2/3 complex-mediated actin nucleation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043130"(ubiquitin binding)|go:"GO:0043332"(mating projection tip)|go:"GO:0044396"(actin cortical patch organization)|go:"GO:0140224"(SLAC complex)|go:"GO:0140312"(cargo adaptor activity)|go:"GO:1990964"(actin cytoskeleton-regulatory complex)|interpro:IPR001452(Src homology-3)|interpro:IPR007131(SLA1 homology domain 1, SHD1)|interpro:IPR013761(Sterile alpha motif-type)|interpro:IPR029922|interpro:IPR035800|interpro:IPR035821|interpro:IPR036028|rcsb pdb:1SSH|rcsb pdb:1Z9Z|rcsb pdb:2HBP|rcsb pdb:2JT4|rcsb pdb:2V1Q|rcsb pdb:3IDW|dip:DIP-695N|mint:P32790 - - crc64:7FD85AA776407624 comment:Fragment 301-354 interaction with HD1 domain of Sla1 is weaker than for fragment 174-354 containing a GAT domain.|comment:The lsb5 C-terminal NPF domain is essential for interaction with sla1. taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2005/01/21 2014/10/16 rogid:rOQErdx6EVNUSspb6WkbM6T02/I559292 rogid:uDuhC6oqoPZrlUhxWJmBGbozCAs559292 intact-crc:FD02AAC4D200DF1F|rigid:R2pYtOBivV2Ov4JPloCpMBkW0cs false binding-associated region:302-346|binding-associated region:?-? binding-associated region:486-555 - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:Q12446 uniprotkb:P25369 intact:EBI-10022|uniprotkb:D6W2N7|ensemblfungi:YOR181W intact:EBI-10218|uniprotkb:P87004|uniprotkb:Q8NIM9|uniprotkb:D6VQY1|ensemblfungi:YCL034W psi-mi:las17_yeast(display_long)|uniprotkb:LAS17(gene name)|psi-mi:LAS17(display_short)|uniprotkb:YOR181W(locus name) psi-mi:lsb5_yeast(display_long)|uniprotkb:YCL186(orf name)|uniprotkb:YCL34W(orf name)|uniprotkb:YCL034W(locus name)|uniprotkb:LSB5(gene name)|psi-mi:LSB5(display_short) psi-mi:"MI:0018"(two hybrid) Costa et al. (2005) pubmed:15651983 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-491581 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_014824.1|sgd:S000005707|ensemblfungi:YOR181W(gene)|ensemblfungi:YOR181W_mRNA(transcript)|go:"GO:0003779"(actin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0007015"(actin filament organization)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030479"(actin cortical patch)|go:"GO:0032233"(positive regulation of actin filament bundle assembly)|go:"GO:0043332"(mating projection tip)|go:"GO:0045010"(actin nucleation)|go:"GO:0051666"(actin cortical patch localization)|go:"GO:0071933"(Arp2/3 complex binding)|go:"GO:0140224"(SLAC complex)|go:"GO:2000601"(positive regulation of Arp2/3 complex-mediated actin nucleation)|interpro:IPR000697(EVH1)|interpro:IPR003124(Actin-binding WH2)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR027641|interpro:IPR033927|rcsb pdb:1ZUK|dip:DIP-963N|mint:Q12446 refseq:NP_009896.2|sgd:S000000539|ensemblfungi:YCL034W(gene)|ensemblfungi:YCL034W_mRNA(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005938"(cell cortex)|go:"GO:0006897"(endocytosis)|go:"GO:0007015"(actin filament organization)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030479"(actin cortical patch)|go:"GO:0051666"(actin cortical patch localization)|interpro:IPR008942(ENTH/VHS)|interpro:IPR044103|dip:DIP-6258N - crc64:4488355563AA2645 - - taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2005/01/21 2014/10/16 rogid:TfQ256gBQPp1T8Ntu2AvktFnypA559292 rogid:rOQErdx6EVNUSspb6WkbM6T02/I559292 intact-crc:16F5CC747505A578|rigid:vVLaCzItB75iRmOketenbOp2wG0 false binding-associated region:292-422 - - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P25369 uniprotkb:P32790 intact:EBI-10218|uniprotkb:P87004|uniprotkb:Q8NIM9|uniprotkb:D6VQY1|ensemblfungi:YCL034W intact:EBI-17313|uniprotkb:D6VPZ3|ensemblfungi:YBL007C psi-mi:lsb5_yeast(display_long)|uniprotkb:YCL186(orf name)|uniprotkb:YCL34W(orf name)|uniprotkb:YCL034W(locus name)|uniprotkb:LSB5(gene name)|psi-mi:LSB5(display_short) psi-mi:sla1_yeast(display_long)|uniprotkb:YBL0321(orf name)|uniprotkb:YBL007C(locus name)|uniprotkb:SLA1(gene name)|psi-mi:SLA1(display_short) psi-mi:"MI:0096"(pull down) Costa et al. (2005) pubmed:15651983 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-476690 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_009896.2|sgd:S000000539|ensemblfungi:YCL034W(gene)|ensemblfungi:YCL034W_mRNA(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005938"(cell cortex)|go:"GO:0006897"(endocytosis)|go:"GO:0007015"(actin filament organization)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030479"(actin cortical patch)|go:"GO:0051666"(actin cortical patch localization)|interpro:IPR008942(ENTH/VHS)|interpro:IPR044103|dip:DIP-6258N refseq:NP_009546.1|sgd:S000000103|ensemblfungi:YBL007C(gene)|ensemblfungi:YBL007C_mRNA(transcript)|go:"GO:0000147"(actin cortical patch assembly)|go:"GO:0003779"(actin binding)|go:"GO:0005634"(nucleus)|go:"GO:0005886"(plasma membrane)|go:"GO:0005935"(cellular bud neck)|go:"GO:0005938"(cell cortex)|go:"GO:0006897"(endocytosis)|go:"GO:0010008"(endosome membrane)|go:"GO:0016197"(endosomal transport)|go:"GO:0030479"(actin cortical patch)|go:"GO:0034316"(negative regulation of Arp2/3 complex-mediated actin nucleation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043130"(ubiquitin binding)|go:"GO:0043332"(mating projection tip)|go:"GO:0044396"(actin cortical patch organization)|go:"GO:0140224"(SLAC complex)|go:"GO:0140312"(cargo adaptor activity)|go:"GO:1990964"(actin cytoskeleton-regulatory complex)|interpro:IPR001452(Src homology-3)|interpro:IPR007131(SLA1 homology domain 1, SHD1)|interpro:IPR013761(Sterile alpha motif-type)|interpro:IPR029922|interpro:IPR035800|interpro:IPR035821|interpro:IPR036028|rcsb pdb:1SSH|rcsb pdb:1Z9Z|rcsb pdb:2HBP|rcsb pdb:2JT4|rcsb pdb:2V1Q|rcsb pdb:3IDW|dip:DIP-695N|mint:P32790 - - crc64:7FD85AA776407624 comment:The Sla1 HD1 domain alone is necessary and sufficient to support the interaction with Lsb5. taxid:-1(in vitro)|taxid:-1(In vitro) - 2005/01/21 2014/10/16 rogid:rOQErdx6EVNUSspb6WkbM6T02/I559292 rogid:uDuhC6oqoPZrlUhxWJmBGbozCAs559292 intact-crc:D4F8252D0142E6D9|rigid:R2pYtOBivV2Ov4JPloCpMBkW0cs false glutathione s tranferase tag:?-? binding-associated region:486-555(IPR007131)|his tag:486-555 - - psi-mi:"MI:0086"(polyclonal antibody western blot) psi-mi:"MI:0086"(polyclonal antibody western blot) uniprotkb:P25369 uniprotkb:P40994 intact:EBI-10218|uniprotkb:P87004|uniprotkb:Q8NIM9|uniprotkb:D6VQY1|ensemblfungi:YCL034W intact:EBI-2824|uniprotkb:D6W2F5|ensemblfungi:YOR094W psi-mi:lsb5_yeast(display_long)|uniprotkb:YCL186(orf name)|uniprotkb:YCL34W(orf name)|uniprotkb:YCL034W(locus name)|uniprotkb:LSB5(gene name)|psi-mi:LSB5(display_short) psi-mi:arf3_yeast(display_long)|uniprotkb:ARF3(gene name)|psi-mi:ARF3(display_short)|uniprotkb:YOR3172W(orf name)|uniprotkb:YOR094W(locus name) psi-mi:"MI:0096"(pull down) Costa et al. (2005) pubmed:15651983 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-476716 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_009896.2|sgd:S000000539|ensemblfungi:YCL034W(gene)|ensemblfungi:YCL034W_mRNA(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005938"(cell cortex)|go:"GO:0006897"(endocytosis)|go:"GO:0007015"(actin filament organization)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030479"(actin cortical patch)|go:"GO:0051666"(actin cortical patch localization)|interpro:IPR008942(ENTH/VHS)|interpro:IPR044103|dip:DIP-6258N refseq:NP_014737.1|sgd:S000005620|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR006689(ARF/SAR superfamily)|interpro:IPR027417|mint:P40994|interpro:IPR041838|ensemblfungi:YOR094W(gene)|ensemblfungi:YOR094W_mRNA(transcript)|go:"GO:0000282"(cellular bud site selection)|go:"GO:0003729"(mRNA binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005934"(cellular bud tip)|go:"GO:0005935"(cellular bud neck)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0008047"(enzyme activator activity)|go:"GO:0010513"(positive regulation of phosphatidylinositol biosynthetic process)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0036267"(invasive filamentous growth)|go:"GO:0051017"(actin filament bundle assembly)|go:"GO:0051666"(actin cortical patch localization) - - - comment:The arf3 T31N mutation abolishes the interaction with lsb5 taxid:-1(in vitro)|taxid:-1(In vitro) - 2005/01/21 2014/10/16 rogid:rOQErdx6EVNUSspb6WkbM6T02/I559292 rogid:uC+fupPUZajD7m7pLEO/K0Kuudo559292 intact-crc:17BF753ECE811DF6|rigid:ZPCacU29DWFaPrUv7qAEqiJEVo0 false glutathione s tranferase tag:?-? mutation:32-32 - - psi-mi:"MI:0086"(polyclonal antibody western blot) psi-mi:"MI:0086"(polyclonal antibody western blot) uniprotkb:P32790 uniprotkb:P25369 intact:EBI-17313|uniprotkb:D6VPZ3|ensemblfungi:YBL007C intact:EBI-10218|uniprotkb:P87004|uniprotkb:Q8NIM9|uniprotkb:D6VQY1|ensemblfungi:YCL034W psi-mi:sla1_yeast(display_long)|uniprotkb:YBL0321(orf name)|uniprotkb:YBL007C(locus name)|uniprotkb:SLA1(gene name)|psi-mi:SLA1(display_short) psi-mi:lsb5_yeast(display_long)|uniprotkb:YCL186(orf name)|uniprotkb:YCL34W(orf name)|uniprotkb:YCL034W(locus name)|uniprotkb:LSB5(gene name)|psi-mi:LSB5(display_short) psi-mi:"MI:0096"(pull down) Costa et al. (2005) pubmed:15651983 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-491465 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_009546.1|sgd:S000000103|ensemblfungi:YBL007C(gene)|ensemblfungi:YBL007C_mRNA(transcript)|go:"GO:0000147"(actin cortical patch assembly)|go:"GO:0003779"(actin binding)|go:"GO:0005634"(nucleus)|go:"GO:0005886"(plasma membrane)|go:"GO:0005935"(cellular bud neck)|go:"GO:0005938"(cell cortex)|go:"GO:0006897"(endocytosis)|go:"GO:0010008"(endosome membrane)|go:"GO:0016197"(endosomal transport)|go:"GO:0030479"(actin cortical patch)|go:"GO:0034316"(negative regulation of Arp2/3 complex-mediated actin nucleation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043130"(ubiquitin binding)|go:"GO:0043332"(mating projection tip)|go:"GO:0044396"(actin cortical patch organization)|go:"GO:0140224"(SLAC complex)|go:"GO:0140312"(cargo adaptor activity)|go:"GO:1990964"(actin cytoskeleton-regulatory complex)|interpro:IPR001452(Src homology-3)|interpro:IPR007131(SLA1 homology domain 1, SHD1)|interpro:IPR013761(Sterile alpha motif-type)|interpro:IPR029922|interpro:IPR035800|interpro:IPR035821|interpro:IPR036028|rcsb pdb:1SSH|rcsb pdb:1Z9Z|rcsb pdb:2HBP|rcsb pdb:2JT4|rcsb pdb:2V1Q|rcsb pdb:3IDW|dip:DIP-695N|mint:P32790 refseq:NP_009896.2|sgd:S000000539|ensemblfungi:YCL034W(gene)|ensemblfungi:YCL034W_mRNA(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005938"(cell cortex)|go:"GO:0006897"(endocytosis)|go:"GO:0007015"(actin filament organization)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030479"(actin cortical patch)|go:"GO:0051666"(actin cortical patch localization)|interpro:IPR008942(ENTH/VHS)|interpro:IPR044103|dip:DIP-6258N - crc64:7FD85AA776407624 - - taxid:-1(in vitro)|taxid:-1(In vitro) - 2005/01/21 2014/10/16 rogid:uDuhC6oqoPZrlUhxWJmBGbozCAs559292 rogid:rOQErdx6EVNUSspb6WkbM6T02/I559292 intact-crc:42AA459EB3B8B8DC|rigid:R2pYtOBivV2Ov4JPloCpMBkW0cs false his tag:486-731|binding-associated region:486-731 glutathione s tranferase tag:?-? - - psi-mi:"MI:0086"(polyclonal antibody western blot) psi-mi:"MI:0086"(polyclonal antibody western blot) uniprotkb:P25369 uniprotkb:P40994 intact:EBI-10218|uniprotkb:P87004|uniprotkb:Q8NIM9|uniprotkb:D6VQY1|ensemblfungi:YCL034W intact:EBI-2824|uniprotkb:D6W2F5|ensemblfungi:YOR094W psi-mi:lsb5_yeast(display_long)|uniprotkb:YCL186(orf name)|uniprotkb:YCL34W(orf name)|uniprotkb:YCL034W(locus name)|uniprotkb:LSB5(gene name)|psi-mi:LSB5(display_short) psi-mi:arf3_yeast(display_long)|uniprotkb:ARF3(gene name)|psi-mi:ARF3(display_short)|uniprotkb:YOR3172W(orf name)|uniprotkb:YOR094W(locus name) psi-mi:"MI:0096"(pull down) Costa et al. (2005) pubmed:15651983 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-491486 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_009896.2|sgd:S000000539|ensemblfungi:YCL034W(gene)|ensemblfungi:YCL034W_mRNA(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005938"(cell cortex)|go:"GO:0006897"(endocytosis)|go:"GO:0007015"(actin filament organization)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030479"(actin cortical patch)|go:"GO:0051666"(actin cortical patch localization)|interpro:IPR008942(ENTH/VHS)|interpro:IPR044103|dip:DIP-6258N refseq:NP_014737.1|sgd:S000005620|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR006689(ARF/SAR superfamily)|interpro:IPR027417|mint:P40994|interpro:IPR041838|ensemblfungi:YOR094W(gene)|ensemblfungi:YOR094W_mRNA(transcript)|go:"GO:0000282"(cellular bud site selection)|go:"GO:0003729"(mRNA binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005934"(cellular bud tip)|go:"GO:0005935"(cellular bud neck)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0008047"(enzyme activator activity)|go:"GO:0010513"(positive regulation of phosphatidylinositol biosynthetic process)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0036267"(invasive filamentous growth)|go:"GO:0051017"(actin filament bundle assembly)|go:"GO:0051666"(actin cortical patch localization) - - - - taxid:-1(in vitro)|taxid:-1(In vitro) - 2005/01/21 2014/10/16 rogid:rOQErdx6EVNUSspb6WkbM6T02/I559292 rogid:uC+fupPUZajD7m7pLEO/K0Kuudo559292 intact-crc:FA271DA8FAE9B3A5|rigid:ZPCacU29DWFaPrUv7qAEqiJEVo0 false - glutathione s tranferase tag:?-? - - psi-mi:"MI:0086"(polyclonal antibody western blot) psi-mi:"MI:0086"(polyclonal antibody western blot) uniprotkb:P25369 uniprotkb:Q12446 intact:EBI-10218|uniprotkb:P87004|uniprotkb:Q8NIM9|uniprotkb:D6VQY1|ensemblfungi:YCL034W intact:EBI-10022|uniprotkb:D6W2N7|ensemblfungi:YOR181W psi-mi:lsb5_yeast(display_long)|uniprotkb:YCL186(orf name)|uniprotkb:YCL34W(orf name)|uniprotkb:YCL034W(locus name)|uniprotkb:LSB5(gene name)|psi-mi:LSB5(display_short) psi-mi:las17_yeast(display_long)|uniprotkb:LAS17(gene name)|psi-mi:LAS17(display_short)|uniprotkb:YOR181W(locus name) psi-mi:"MI:0018"(two hybrid) Costa et al. (2005) pubmed:15651983 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-476701 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_009896.2|sgd:S000000539|ensemblfungi:YCL034W(gene)|ensemblfungi:YCL034W_mRNA(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005938"(cell cortex)|go:"GO:0006897"(endocytosis)|go:"GO:0007015"(actin filament organization)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030479"(actin cortical patch)|go:"GO:0051666"(actin cortical patch localization)|interpro:IPR008942(ENTH/VHS)|interpro:IPR044103|dip:DIP-6258N refseq:NP_014824.1|sgd:S000005707|ensemblfungi:YOR181W(gene)|ensemblfungi:YOR181W_mRNA(transcript)|go:"GO:0003779"(actin binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0007015"(actin filament organization)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030479"(actin cortical patch)|go:"GO:0032233"(positive regulation of actin filament bundle assembly)|go:"GO:0043332"(mating projection tip)|go:"GO:0045010"(actin nucleation)|go:"GO:0051666"(actin cortical patch localization)|go:"GO:0071933"(Arp2/3 complex binding)|go:"GO:0140224"(SLAC complex)|go:"GO:2000601"(positive regulation of Arp2/3 complex-mediated actin nucleation)|interpro:IPR000697(EVH1)|interpro:IPR003124(Actin-binding WH2)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR027641|interpro:IPR033927|rcsb pdb:1ZUK|dip:DIP-963N|mint:Q12446 - - crc64:4488355563AA2645 - taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2005/01/21 2014/10/16 rogid:rOQErdx6EVNUSspb6WkbM6T02/I559292 rogid:TfQ256gBQPp1T8Ntu2AvktFnypA559292 intact-crc:9BAC6724108192D3|rigid:vVLaCzItB75iRmOketenbOp2wG0 false - binding-associated region:292-536 - - psi-mi:"MI:0078"(nucleotide sequence identification) psi-mi:"MI:0078"(nucleotide sequence identification) uniprotkb:P25369 intact:EBI-19797 intact:EBI-10218|uniprotkb:P87004|uniprotkb:Q8NIM9|uniprotkb:D6VQY1|ensemblfungi:YCL034W dip:DIP-1549N|uniprotkb:P04838|uniprotkb:Q6LA96 psi-mi:lsb5_yeast(display_long)|uniprotkb:YCL186(orf name)|uniprotkb:YCL34W(orf name)|uniprotkb:YCL034W(locus name)|uniprotkb:LSB5(gene name)|psi-mi:LSB5(display_short) psi-mi:ubiq_yeast(display_short)|psi-mi:EBI-19797(display_long)|uniprotkb:UBI1(gene name)|psi-mi:UBI1(display_short)|uniprotkb:RPL40A(gene name synonym)|uniprotkb:YIL148W(locus name)|uniprotkb:UBI2(gene name)|psi-mi:UBI2(display_short)|uniprotkb:RPL40B(gene name synonym)|uniprotkb:YKR094C(locus name)|uniprotkb:UBI3(gene name)|psi-mi:UBI3(display_short)|uniprotkb:RPS31(gene name synonym)|uniprotkb:YLR167W(locus name)|uniprotkb:L9470.14(orf name)|uniprotkb:UBI4(gene name)|psi-mi:UBI4(display_short)|uniprotkb:SCD2(gene name synonym)|uniprotkb:YLL039C(locus name) psi-mi:"MI:0096"(pull down) Costa et al. (2005) pubmed:15651983 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-476732 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_009896.2|sgd:S000000539|ensemblfungi:YCL034W(gene)|ensemblfungi:YCL034W_mRNA(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005938"(cell cortex)|go:"GO:0006897"(endocytosis)|go:"GO:0007015"(actin filament organization)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030479"(actin cortical patch)|go:"GO:0051666"(actin cortical patch localization)|interpro:IPR008942(ENTH/VHS)|interpro:IPR044103|dip:DIP-6258N sgd:S000001410(RPL40A)|sgd:S000001802(RPL40B)|sgd:S000004157(RPS31)|sgd:S000003962(UBI4)|uniprotkb:P61864(see-also)|refseq:NP_012118.1|refseq:NP_013020.1|refseq:NP_013061.1|refseq:NP_013268.1|ensembl:YIL148W|ensembl:YKR094C|ensembl:YLR167W|go:"GO:0005515"(protein binding)|interpro:IPR000626(Ubiquitin)|cygd:YLR167w|go:"GO:0022625"(cytosolic large ribosomal subunit)|go:"GO:0005739"(mitochondrion)|go:"GO:0003735"(structural constituent of ribosome)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0042254"(ribosome biogenesis)|go:"GO:0006412"(translation)|ensembl:YLL039C|rcsb pdb:1OTR|rcsb pdb:1Q0W|rcsb pdb:1WR1|rcsb pdb:1ZW7|rcsb pdb:2G3Q|go:"GO:0005634"(nucleus)|go:"GO:0006281"(DNA repair)|cygd:YIL148w|cygd:YKR094c|cygd:YLL039c|rcsb pdb:2JT4|rcsb pdb:2JWZ|rcsb pdb:3CMM|interpro:IPR019954|interpro:IPR019956|interpro:IPR019955|rcsb pdb:3L0W|rcsb pdb:3L10|rcsb pdb:3MHS|rcsb pdb:2KDI - - no-uniprot-update:obsolete protein - taxid:-1(in vitro)|taxid:-1(In vitro) - 2005/01/21 2014/10/16 rogid:rOQErdx6EVNUSspb6WkbM6T02/I559292 rogid:sUFDb7kGedoC/k80bI4sjOaMLHs4932 intact-crc:92B5A7D116976FE1|rigid:rdWiKR1QUKFfk6iZZiEME/mu0D0 false - glutathione s tranferase tag:?-? - - psi-mi:"MI:0086"(polyclonal antibody western blot) psi-mi:"MI:0086"(polyclonal antibody western blot)