#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P0ACD4 uniprotkb:P0A6B7 intact:EBI-561646|ensemblbacteria:AAC75582|ensemblbacteria:BAA16423|uniprotkb:P77310 intact:EBI-550055|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAT48142|ensemblbacteria:BAA16424 psi-mi:iscu_ecoli(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:yfhN(gene name synonym)|uniprotkb:JW2513(locus name)|uniprotkb:b2529(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym) psi-mi:iscs_ecoli(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:b2530(locus name)|uniprotkb:JW2514(locus name)|uniprotkb:yfhO(gene name synonym)|uniprotkb:yzzO(gene name synonym)|uniprotkb:ThiI transpersulfidase(gene name synonym)|uniprotkb:NifS protein homolog(gene name synonym)|uniprotkb:TusA transpersulfidase(gene name synonym)|uniprotkb:nuvC(gene name synonym) psi-mi:"MI:0096"(pull down) Kato et al. (2002) pubmed:11972033 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0195"(covalent binding) psi-mi:"MI:0469"(IntAct) intact:EBI-604068 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblbacteria:AAC75582(transcript)|ensemblbacteria:b2529(gene)|ensemblbacteria:BAA16423(gene)|ensemblbacteria:BAA16423(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006879"(cellular iron ion homeostasis)|go:"GO:0008198"(ferrous iron binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0042802"(identical protein binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:2KQK|rcsb pdb:2L4X|refseq:NP_417024.1|refseq:WP_000331707.1|dip:DIP-48025N refseq:YP_026169.1|refseq:WP_001295373.1|dip:DIP-29109N|ensemblbacteria:AAT48142(transcript)|ensemblbacteria:b2530(gene)|ensemblbacteria:BAA16424(gene)|ensemblbacteria:BAA16424(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0009000"(selenocysteine lyase activity)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0018131"(oxazole or thiazole biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0031119"(tRNA pseudouridine synthesis)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:1P3W - - crc64:1E4F2E6F9CD266B0 comment:The proteins are linked by a disulfide bond as the complex is sensitive to beta-mercaptoethanol bait and prey are overexpressed taxid:-1(in vitro)|taxid:-1(In vitro) - 2005/06/22 2014/10/16 rogid:2JmTdVPfAy33rAim10pVQ33gJrI83333 rogid:BbF93e6Jza5CijszN1IFt/7B2BY83333 intact-crc:FE7501B970E76BFB|rigid:bUfLz38DhIojFivNgSUcHgdI9uA false his tag:c-c - - - psi-mi:"MI:0039"(edman degradation) psi-mi:"MI:0039"(edman degradation) uniprotkb:P0ACD4 uniprotkb:P0ACD4 intact:EBI-561646|ensemblbacteria:AAC75582|ensemblbacteria:BAA16423|uniprotkb:P77310 intact:EBI-561646|ensemblbacteria:AAC75582|ensemblbacteria:BAA16423|uniprotkb:P77310 psi-mi:iscu_ecoli(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:yfhN(gene name synonym)|uniprotkb:JW2513(locus name)|uniprotkb:b2529(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym) psi-mi:iscu_ecoli(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:yfhN(gene name synonym)|uniprotkb:JW2513(locus name)|uniprotkb:b2529(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym) psi-mi:"MI:0096"(pull down) Kato et al. (2002) pubmed:11972033 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0195"(covalent binding) psi-mi:"MI:0469"(IntAct) intact:EBI-607992 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblbacteria:AAC75582(transcript)|ensemblbacteria:b2529(gene)|ensemblbacteria:BAA16423(gene)|ensemblbacteria:BAA16423(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006879"(cellular iron ion homeostasis)|go:"GO:0008198"(ferrous iron binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0042802"(identical protein binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:2KQK|rcsb pdb:2L4X|refseq:NP_417024.1|refseq:WP_000331707.1|dip:DIP-48025N ensemblbacteria:AAC75582(transcript)|ensemblbacteria:b2529(gene)|ensemblbacteria:BAA16423(gene)|ensemblbacteria:BAA16423(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006879"(cellular iron ion homeostasis)|go:"GO:0008198"(ferrous iron binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0042802"(identical protein binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:2KQK|rcsb pdb:2L4X|refseq:NP_417024.1|refseq:WP_000331707.1|dip:DIP-48025N - - - comment:The proteins are linked by a disulfide bond as the complex is sensitive to beta-mercaptoethanol homodimer proteins are overexpressed taxid:-1(in vitro)|taxid:-1(In vitro) - 2005/06/22 2014/10/16 rogid:2JmTdVPfAy33rAim10pVQ33gJrI83333 rogid:2JmTdVPfAy33rAim10pVQ33gJrI83333 intact-crc:726453ABAE52C536|rigid:nNmbZ5/1sgHYTEy78uCqJPxQ6Ek false his tag:c-c - - - psi-mi:"MI:0039"(edman degradation) psi-mi:"MI:0039"(edman degradation) uniprotkb:P0A6B7 uniprotkb:P0ACD4 intact:EBI-550055|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAT48142|ensemblbacteria:BAA16424 intact:EBI-561646|ensemblbacteria:AAC75582|ensemblbacteria:BAA16423|uniprotkb:P77310 psi-mi:iscs_ecoli(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:b2530(locus name)|uniprotkb:JW2514(locus name)|uniprotkb:yfhO(gene name synonym)|uniprotkb:yzzO(gene name synonym)|uniprotkb:ThiI transpersulfidase(gene name synonym)|uniprotkb:NifS protein homolog(gene name synonym)|uniprotkb:TusA transpersulfidase(gene name synonym)|uniprotkb:nuvC(gene name synonym) psi-mi:iscu_ecoli(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:yfhN(gene name synonym)|uniprotkb:JW2513(locus name)|uniprotkb:b2529(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym) psi-mi:"MI:0096"(pull down) Kato et al. (2002) pubmed:11972033 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-604135 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:YP_026169.1|refseq:WP_001295373.1|dip:DIP-29109N|ensemblbacteria:AAT48142(transcript)|ensemblbacteria:b2530(gene)|ensemblbacteria:BAA16424(gene)|ensemblbacteria:BAA16424(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0009000"(selenocysteine lyase activity)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0018131"(oxazole or thiazole biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0031119"(tRNA pseudouridine synthesis)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:1P3W ensemblbacteria:AAC75582(transcript)|ensemblbacteria:b2529(gene)|ensemblbacteria:BAA16423(gene)|ensemblbacteria:BAA16423(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006879"(cellular iron ion homeostasis)|go:"GO:0008198"(ferrous iron binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0042802"(identical protein binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:2KQK|rcsb pdb:2L4X|refseq:NP_417024.1|refseq:WP_000331707.1|dip:DIP-48025N - crc64:1E4F2E6F9CD266B0 - comment:"stoichiometry is 1:1. size of the complex was 64kDa."|comment:proteins over expressed taxid:-1(in vitro)|taxid:-1(In vitro) - 2005/06/22 2014/10/16 rogid:BbF93e6Jza5CijszN1IFt/7B2BY83333 rogid:2JmTdVPfAy33rAim10pVQ33gJrI83333 intact-crc:4D81955AA3777385|rigid:bUfLz38DhIojFivNgSUcHgdI9uA false - his tag:c-c 1 1 psi-mi:"MI:0039"(edman degradation) psi-mi:"MI:0039"(edman degradation) uniprotkb:P0ACD4 uniprotkb:P0ACD4 intact:EBI-561646|ensemblbacteria:AAC75582|ensemblbacteria:BAA16423|uniprotkb:P77310 intact:EBI-561646|ensemblbacteria:AAC75582|ensemblbacteria:BAA16423|uniprotkb:P77310 psi-mi:iscu_ecoli(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:yfhN(gene name synonym)|uniprotkb:JW2513(locus name)|uniprotkb:b2529(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym) psi-mi:iscu_ecoli(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:yfhN(gene name synonym)|uniprotkb:JW2513(locus name)|uniprotkb:b2529(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym) psi-mi:"MI:0096"(pull down) Kato et al. (2002) pubmed:11972033 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-604144 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblbacteria:AAC75582(transcript)|ensemblbacteria:b2529(gene)|ensemblbacteria:BAA16423(gene)|ensemblbacteria:BAA16423(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006879"(cellular iron ion homeostasis)|go:"GO:0008198"(ferrous iron binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0042802"(identical protein binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:2KQK|rcsb pdb:2L4X|refseq:NP_417024.1|refseq:WP_000331707.1|dip:DIP-48025N ensemblbacteria:AAC75582(transcript)|ensemblbacteria:b2529(gene)|ensemblbacteria:BAA16423(gene)|ensemblbacteria:BAA16423(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006879"(cellular iron ion homeostasis)|go:"GO:0008198"(ferrous iron binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0042802"(identical protein binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:2KQK|rcsb pdb:2L4X|refseq:NP_417024.1|refseq:WP_000331707.1|dip:DIP-48025N - - - comment:"stoichiometry is 1:1. size of the complex was 38kDa."|comment:proteins overexpressed. taxid:-1(in vitro)|taxid:-1(In vitro) - 2005/06/22 2014/10/16 rogid:2JmTdVPfAy33rAim10pVQ33gJrI83333 rogid:2JmTdVPfAy33rAim10pVQ33gJrI83333 intact-crc:EDF90C783D916202|rigid:nNmbZ5/1sgHYTEy78uCqJPxQ6Ek false his tag:c-c his tag:c-c 1 1 psi-mi:"MI:0039"(edman degradation) psi-mi:"MI:0039"(edman degradation) uniprotkb:P0ACD4 uniprotkb:P0A6B7 intact:EBI-561646|ensemblbacteria:AAC75582|ensemblbacteria:BAA16423|uniprotkb:P77310 intact:EBI-550055|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAT48142|ensemblbacteria:BAA16424 psi-mi:iscu_ecoli(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:yfhN(gene name synonym)|uniprotkb:JW2513(locus name)|uniprotkb:b2529(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym) psi-mi:iscs_ecoli(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:b2530(locus name)|uniprotkb:JW2514(locus name)|uniprotkb:yfhO(gene name synonym)|uniprotkb:yzzO(gene name synonym)|uniprotkb:ThiI transpersulfidase(gene name synonym)|uniprotkb:NifS protein homolog(gene name synonym)|uniprotkb:TusA transpersulfidase(gene name synonym)|uniprotkb:nuvC(gene name synonym) psi-mi:"MI:0096"(pull down) Kato et al. (2002) pubmed:11972033 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-604153 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblbacteria:AAC75582(transcript)|ensemblbacteria:b2529(gene)|ensemblbacteria:BAA16423(gene)|ensemblbacteria:BAA16423(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006879"(cellular iron ion homeostasis)|go:"GO:0008198"(ferrous iron binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0042802"(identical protein binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:2KQK|rcsb pdb:2L4X|refseq:NP_417024.1|refseq:WP_000331707.1|dip:DIP-48025N refseq:YP_026169.1|refseq:WP_001295373.1|dip:DIP-29109N|ensemblbacteria:AAT48142(transcript)|ensemblbacteria:b2530(gene)|ensemblbacteria:BAA16424(gene)|ensemblbacteria:BAA16424(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0009000"(selenocysteine lyase activity)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0018131"(oxazole or thiazole biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0031119"(tRNA pseudouridine synthesis)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:1P3W - - crc64:1E4F2E6F9CD266B0 comment:Size of the complex was 150kDa.|comment:Interacting Cys were identified by cyanolysis, identification of the crosslinked residues was done by digestion of the complex followed by reducing the disulfide bond and detection by ABD-f labelling.|comment:Mutation analysis indicated that though the disulfide bond formed was between Cys-328 of Iscs and Cys-63 of IscU the specific interaction did not require these residues. taxid:-1(in vitro)|taxid:-1(In vitro) - 2005/06/22 2014/10/16 rogid:2JmTdVPfAy33rAim10pVQ33gJrI83333 rogid:BbF93e6Jza5CijszN1IFt/7B2BY83333 intact-crc:1193188A0E853C0C|rigid:bUfLz38DhIojFivNgSUcHgdI9uA false his tag:c-c|binding-associated region:64-64 binding-associated region:329-329 2 2 psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P0ACD4 uniprotkb:P0ACD4 intact:EBI-561646|ensemblbacteria:AAC75582|ensemblbacteria:BAA16423|uniprotkb:P77310 intact:EBI-561646|ensemblbacteria:AAC75582|ensemblbacteria:BAA16423|uniprotkb:P77310 psi-mi:iscu_ecoli(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:yfhN(gene name synonym)|uniprotkb:JW2513(locus name)|uniprotkb:b2529(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym) psi-mi:iscu_ecoli(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:yfhN(gene name synonym)|uniprotkb:JW2513(locus name)|uniprotkb:b2529(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym) psi-mi:"MI:0096"(pull down) Kato et al. (2002) pubmed:11972033 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-604159 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblbacteria:AAC75582(transcript)|ensemblbacteria:b2529(gene)|ensemblbacteria:BAA16423(gene)|ensemblbacteria:BAA16423(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006879"(cellular iron ion homeostasis)|go:"GO:0008198"(ferrous iron binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0042802"(identical protein binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:2KQK|rcsb pdb:2L4X|refseq:NP_417024.1|refseq:WP_000331707.1|dip:DIP-48025N ensemblbacteria:AAC75582(transcript)|ensemblbacteria:b2529(gene)|ensemblbacteria:BAA16423(gene)|ensemblbacteria:BAA16423(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006879"(cellular iron ion homeostasis)|go:"GO:0008198"(ferrous iron binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0042802"(identical protein binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:2KQK|rcsb pdb:2L4X|refseq:NP_417024.1|refseq:WP_000331707.1|dip:DIP-48025N - - - comment:"stoichiometry is 1:1. size of the complex was 45kDa." taxid:-1(in vitro)|taxid:-1(In vitro) - 2005/06/22 2014/10/16 rogid:2JmTdVPfAy33rAim10pVQ33gJrI83333 rogid:2JmTdVPfAy33rAim10pVQ33gJrI83333 intact-crc:0822774C29DDDDC8|rigid:nNmbZ5/1sgHYTEy78uCqJPxQ6Ek false his tag:c-c his tag:c-c 1 1 psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot)