#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q03497	uniprotkb:P19073	intact:EBI-18285|uniprotkb:D3DKQ8|ensemblfungi:YHL007C	intact:EBI-4274|uniprotkb:Q05978|uniprotkb:D6VYN1|ensemblfungi:YLR229C	psi-mi:ste20_yeast(display_long)|uniprotkb:STE20(gene name)|psi-mi:STE20(display_short)|uniprotkb:YHL007C(locus name)	psi-mi:cdc42_yeast(display_long)|uniprotkb:CDC42(gene name)|psi-mi:CDC42(display_short)|uniprotkb:SRO2(gene name synonym)|uniprotkb:YLR229C(locus name)|uniprotkb:L8083.13(orf name)|uniprotkb:Suppressor of RHO3 protein 2(gene name synonym)	psi-mi:"MI:0018"(two hybrid)	Lamson et al. (2002)	pubmed:11940652	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-526223	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_011856.1|sgd:S000000999|ensemblfungi:YHL007C(gene)|ensemblfungi:YHL007C_mRNA(transcript)|go:"GO:0000011"(vacuole inheritance)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000131"(incipient cellular bud site)|go:"GO:0000282"(cellular bud site selection)|go:"GO:0000750"(pheromone-dependent signal transduction involved in conjugation with cellular fusion)|go:"GO:0001402"(signal transduction involved in filamentous growth)|reactome:R-SCE-389359|interpro:IPR036936|reactome:R-SCE-5627123|reactome:R-SCE-5687128|reactome:R-SCE-9013405|reactome:R-SCE-9013406|rcsb pdb:2KYM|rcsb pdb:2LCS|rcsb pdb:2RQW|reactome:R-SCE-2029482|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007096"(regulation of exit from mitosis)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0003729"(mRNA binding)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0007118"(budding cell apical bud growth)|go:"GO:0007121"(bipolar cellular bud site selection)|go:"GO:0007124"(pseudohyphal growth)|go:"GO:0007232"(osmosensory signaling pathway via Sho1 osmosensor)|go:"GO:0010468"(regulation of gene expression)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0019899"(enzyme binding)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0034063"(stress granule assembly)|go:"GO:0035376"(sterol import)|go:"GO:0035404"(histone-serine phosphorylation)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043332"(mating projection tip)|go:"GO:0043408"(regulation of MAPK cascade)|go:"GO:0044025"("histone kinase activity (H2B-S14 specific)")|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000095(PAK-box/P21-Rho-binding)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR033923|dip:DIP-712N|mint:Q03497	dip:DIP-862N|refseq:NP_013330.1|sgd:S000004219|mint:P19073|ensemblfungi:YLR229C(gene)|ensemblfungi:YLR229C_mRNA(transcript)|go:"GO:0000131"(incipient cellular bud site)|go:"GO:0000329"(fungal-type vacuole membrane)|go:"GO:0000747"(conjugation with cellular fusion)|go:"GO:0000750"(pheromone-dependent signal transduction involved in conjugation with cellular fusion)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0005934"(cellular bud tip)|go:"GO:0005935"(cellular bud neck)|go:"GO:0005940"(septin ring)|go:"GO:0006897"(endocytosis)|go:"GO:0007049"(cell cycle)|go:"GO:0007096"(regulation of exit from mitosis)|go:"GO:0007118"(budding cell apical bud growth)|go:"GO:0007119"(budding cell isotropic bud growth)|go:"GO:0007266"(Rho protein signal transduction)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030010"(establishment of cell polarity)|go:"GO:0030031"(cell projection assembly)|go:"GO:0031106"(septin ring organization)|go:"GO:0031384"(regulation of initiation of mating projection growth)|go:"GO:0031965"(nuclear membrane)|go:"GO:0032488"(Cdc42 protein signal transduction)|go:"GO:0032889"(regulation of vacuole fusion, non-autophagic)|go:"GO:0043332"(mating projection tip)|go:"GO:0045921"(positive regulation of exocytosis)|go:"GO:0060178"(regulation of exocyst localization)|go:"GO:0071944"(cell periphery)|go:"GO:2000222"(positive regulation of pseudohyphal growth)|interpro:IPR001806(Ras GTPase)|interpro:IPR003578(Ras small GTPase, Rho type)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR027417|interpro:IPR037874|reactome:R-SCE-2029482|reactome:R-SCE-389359|reactome:R-SCE-5626467|reactome:R-SCE-5627123|reactome:R-SCE-5687128|reactome:R-SCE-9013148|reactome:R-SCE-9013406|reactome:R-SCE-9013409|reactome:R-SCE-983231	-	crc64:69C1C12F5B87733C	crc64:30BD644BA9836B2C	comment:Gly12Val Cdc42 is constitutively active GTP-bound so it can binds to effectors like ste20.|comment:Cys188Ser Cdc42 is not lipid-modified so it can be used in the Y2H system.|comment:S338A mutation in Ste20 decreased interaction with Bem1. H645G mutation had a stronger effect. The double mutation showed the strongest effect.	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2005/04/06	2014/10/16	rogid:ammX6PwJCeyVjNxJ2UJUBrZt9mM559292	rogid:4FDrNURFjSZOYUFf/dADXTpoMTc559292	intact-crc:EC1BDC4926DCFC80|rigid:7L3BdeqLSKwAstxYPP8TnThrHa4	false	necessary binding region:334-369(IPR000095)|mutation decreasing interaction:338-338,345-345	mutation:12-12,188-188	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P29366	uniprotkb:Q03497	intact:EBI-3508|uniprotkb:D6VQJ6|ensemblfungi:YBR200W	intact:EBI-18285|uniprotkb:D3DKQ8|ensemblfungi:YHL007C	psi-mi:bem1_yeast(display_long)|uniprotkb:Suppressor of RHO3 protein 1(gene name synonym)|uniprotkb:BEM1(gene name)|psi-mi:BEM1(display_short)|uniprotkb:SRO1(gene name synonym)|uniprotkb:YBR200W(locus name)|uniprotkb:YBR1412(orf name)	psi-mi:ste20_yeast(display_long)|uniprotkb:STE20(gene name)|psi-mi:STE20(display_short)|uniprotkb:YHL007C(locus name)	psi-mi:"MI:0018"(two hybrid)	Lamson et al. (2002)	pubmed:11940652	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-526429	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000000404|refseq:NP_009759.3|dip:DIP-688N|mint:P29366|ensemblfungi:YBR200W(gene)|ensemblfungi:YBR200W_mRNA(transcript)|go:"GO:0000131"(incipient cellular bud site)|go:"GO:0000747"(conjugation with cellular fusion)|go:"GO:0000753"(cell morphogenesis involved in conjugation with cellular fusion)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005856"(cytoskeleton)|go:"GO:0005934"(cellular bud tip)|go:"GO:0005935"(cellular bud neck)|go:"GO:0005938"(cell cortex)|go:"GO:0006801"(superoxide metabolic process)|go:"GO:0016176"(superoxide-generating NADPH oxidase activator activity)|go:"GO:0030427"(site of polarized growth)|go:"GO:0032266"(phosphatidylinositol-3-phosphate binding)|go:"GO:0043332"(mating projection tip)|go:"GO:0045185"(maintenance of protein location)|go:"GO:0060090"(molecular adaptor activity)|interpro:IPR000270(Octicosapeptide/Phox/Bem1p)|interpro:IPR001452(Src homology-3)|interpro:IPR001683(Phox-like)|interpro:IPR030507|interpro:IPR035548|interpro:IPR035549|interpro:IPR035550|interpro:IPR036028|interpro:IPR036871|rcsb pdb:1IP9|rcsb pdb:1IPG|rcsb pdb:2CZO|rcsb pdb:2KFK|rcsb pdb:2RQV|rcsb pdb:2RQW|rcsb pdb:2V6V|reactome:R-SCE-5668599	refseq:NP_011856.1|sgd:S000000999|ensemblfungi:YHL007C(gene)|ensemblfungi:YHL007C_mRNA(transcript)|go:"GO:0000011"(vacuole inheritance)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000131"(incipient cellular bud site)|go:"GO:0000282"(cellular bud site selection)|go:"GO:0000750"(pheromone-dependent signal transduction involved in conjugation with cellular fusion)|go:"GO:0001402"(signal transduction involved in filamentous growth)|reactome:R-SCE-389359|interpro:IPR036936|reactome:R-SCE-5627123|reactome:R-SCE-5687128|reactome:R-SCE-9013405|reactome:R-SCE-9013406|rcsb pdb:2KYM|rcsb pdb:2LCS|rcsb pdb:2RQW|reactome:R-SCE-2029482|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007096"(regulation of exit from mitosis)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0003729"(mRNA binding)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0007118"(budding cell apical bud growth)|go:"GO:0007121"(bipolar cellular bud site selection)|go:"GO:0007124"(pseudohyphal growth)|go:"GO:0007232"(osmosensory signaling pathway via Sho1 osmosensor)|go:"GO:0010468"(regulation of gene expression)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0019899"(enzyme binding)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0034063"(stress granule assembly)|go:"GO:0035376"(sterol import)|go:"GO:0035404"(histone-serine phosphorylation)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043332"(mating projection tip)|go:"GO:0043408"(regulation of MAPK cascade)|go:"GO:0044025"("histone kinase activity (H2B-S14 specific)")|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000095(PAK-box/P21-Rho-binding)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR033923|dip:DIP-712N|mint:Q03497	-	crc64:FB6C525AE9A19181	crc64:69C1C12F5B87733C	-	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2005/04/06	2014/10/16	rogid:Fq7WxTviE31ivPWGJoZO5DsUcdg559292	rogid:ammX6PwJCeyVjNxJ2UJUBrZt9mM559292	intact-crc:6DF10C332AAA7789|rigid:QCJqHrvfSXBiFYAe6jPo48g8k5Y	false	binding-associated region:157-551	binding-associated region:1-499	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q03497	uniprotkb:Q03497	intact:EBI-18285|uniprotkb:D3DKQ8|ensemblfungi:YHL007C	intact:EBI-18285|uniprotkb:D3DKQ8|ensemblfungi:YHL007C	psi-mi:ste20_yeast(display_long)|uniprotkb:STE20(gene name)|psi-mi:STE20(display_short)|uniprotkb:YHL007C(locus name)	psi-mi:ste20_yeast(display_long)|uniprotkb:STE20(gene name)|psi-mi:STE20(display_short)|uniprotkb:YHL007C(locus name)	psi-mi:"MI:0018"(two hybrid)	Lamson et al. (2002)	pubmed:11940652	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-526452	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_011856.1|sgd:S000000999|ensemblfungi:YHL007C(gene)|ensemblfungi:YHL007C_mRNA(transcript)|go:"GO:0000011"(vacuole inheritance)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000131"(incipient cellular bud site)|go:"GO:0000282"(cellular bud site selection)|go:"GO:0000750"(pheromone-dependent signal transduction involved in conjugation with cellular fusion)|go:"GO:0001402"(signal transduction involved in filamentous growth)|reactome:R-SCE-389359|interpro:IPR036936|reactome:R-SCE-5627123|reactome:R-SCE-5687128|reactome:R-SCE-9013405|reactome:R-SCE-9013406|rcsb pdb:2KYM|rcsb pdb:2LCS|rcsb pdb:2RQW|reactome:R-SCE-2029482|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007096"(regulation of exit from mitosis)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0003729"(mRNA binding)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0007118"(budding cell apical bud growth)|go:"GO:0007121"(bipolar cellular bud site selection)|go:"GO:0007124"(pseudohyphal growth)|go:"GO:0007232"(osmosensory signaling pathway via Sho1 osmosensor)|go:"GO:0010468"(regulation of gene expression)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0019899"(enzyme binding)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0034063"(stress granule assembly)|go:"GO:0035376"(sterol import)|go:"GO:0035404"(histone-serine phosphorylation)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043332"(mating projection tip)|go:"GO:0043408"(regulation of MAPK cascade)|go:"GO:0044025"("histone kinase activity (H2B-S14 specific)")|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000095(PAK-box/P21-Rho-binding)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR033923|dip:DIP-712N|mint:Q03497	refseq:NP_011856.1|sgd:S000000999|ensemblfungi:YHL007C(gene)|ensemblfungi:YHL007C_mRNA(transcript)|go:"GO:0000011"(vacuole inheritance)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000131"(incipient cellular bud site)|go:"GO:0000282"(cellular bud site selection)|go:"GO:0000750"(pheromone-dependent signal transduction involved in conjugation with cellular fusion)|go:"GO:0001402"(signal transduction involved in filamentous growth)|reactome:R-SCE-389359|interpro:IPR036936|reactome:R-SCE-5627123|reactome:R-SCE-5687128|reactome:R-SCE-9013405|reactome:R-SCE-9013406|rcsb pdb:2KYM|rcsb pdb:2LCS|rcsb pdb:2RQW|reactome:R-SCE-2029482|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007096"(regulation of exit from mitosis)|go:"GO:0001403"(invasive growth in response to glucose limitation)|go:"GO:0003729"(mRNA binding)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0007118"(budding cell apical bud growth)|go:"GO:0007121"(bipolar cellular bud site selection)|go:"GO:0007124"(pseudohyphal growth)|go:"GO:0007232"(osmosensory signaling pathway via Sho1 osmosensor)|go:"GO:0010468"(regulation of gene expression)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0019899"(enzyme binding)|go:"GO:0032147"(activation of protein kinase activity)|go:"GO:0034063"(stress granule assembly)|go:"GO:0035376"(sterol import)|go:"GO:0035404"(histone-serine phosphorylation)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0043065"(positive regulation of apoptotic process)|go:"GO:0043332"(mating projection tip)|go:"GO:0043408"(regulation of MAPK cascade)|go:"GO:0044025"("histone kinase activity (H2B-S14 specific)")|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000095(PAK-box/P21-Rho-binding)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR033923|dip:DIP-712N|mint:Q03497	-	crc64:69C1C12F5B87733C	crc64:69C1C12F5B87733C	comment:Ste20 CRIB domain by binding to Ste20 kinase domain acts as an autoinhibitory domain on kinase activity.|inhibition:The interaction between the CRIB and kinase domain is competed by interaction of Cdc42 with CRIB domain. Cdc42 binding to ste20 stimulates Ste20 kinase activity by relief of CRIB inhibition.	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2005/04/06	2014/10/16	rogid:ammX6PwJCeyVjNxJ2UJUBrZt9mM559292	rogid:ammX6PwJCeyVjNxJ2UJUBrZt9mM559292	intact-crc:7C44FA813D89FE3C|rigid:hGqEM+DYO7ODiIICNgXi6QVUmhQ	false	binding-associated region:580-939(IPR002290)	necessary binding region:334-369(IPR000095)|binding-associated region:1-439|mutation decreasing interaction:369-369	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)