matrixdb Institut de Biologie et de Chimie des Prot\u00c3\u00a9ines, 7, passage du Vercors 69 367 Lyon cedex 07 FRANCE matrixdb@ibcp.fr http://matrixdb.ibcp.fr The heparin binding motif of endostatin mediates its interaction with receptor nucleolin. The heparin binding motif of endostatin mediates its interaction with receptor nucleolin. Biochemistry (0006-2960) 2009 imex curation Fu Y., Chen Y., Luo X., Liang Y., Shi H., Gao L., Zhan S., Zhou D., Luo Y. yluo@tsinghua.edu.cn 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro facs fluorescence-activated cell sorting FACS Flow cytometry facs antibody detection identification by antibody antibody detection Fu Y., Chen Y., Luo X., Liang Y., Shi H., Gao L., Zhan S., Zhou D., Luo Y. yluo@tsinghua.edu.cn Accepted 2012-JUL-30 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Biochemistry (0006-2960) 2009 Only protein-protein interactions imex curation The heparin binding motif of endostatin mediates its interaction with receptor nucleolin. The heparin binding motif of endostatin mediates its interaction with receptor nucleolin. Biochemistry (0006-2960) 2009 imex curation Fu Y., Chen Y., Luo X., Liang Y., Shi H., Gao L., Zhan S., Zhou D., Luo Y. yluo@tsinghua.edu.cn 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro fluorescence fluorescence technology fluorescence antibody detection identification by antibody antibody detection Fu Y., Chen Y., Luo X., Liang Y., Shi H., Gao L., Zhan S., Zhou D., Luo Y. yluo@tsinghua.edu.cn Accepted 2012-JUL-30 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Biochemistry (0006-2960) 2009 Only protein-protein interactions imex curation The heparin binding motif of endostatin mediates its interaction with receptor nucleolin. The heparin binding motif of endostatin mediates its interaction with receptor nucleolin. Biochemistry (0006-2960) 2009 imex curation Fu Y., Chen Y., Luo X., Liang Y., Shi H., Gao L., Zhan S., Zhou D., Luo Y. yluo@tsinghua.edu.cn 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Fu Y., Chen Y., Luo X., Liang Y., Shi H., Gao L., Zhan S., Zhou D., Luo Y. yluo@tsinghua.edu.cn Accepted 2012-JUL-30 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Biochemistry (0006-2960) 2009 Only protein-protein interactions imex curation The heparin binding motif of endostatin mediates its interaction with receptor nucleolin. The heparin binding motif of endostatin mediates its interaction with receptor nucleolin. Biochemistry (0006-2960) 2009 imex curation Fu Y., Chen Y., Luo X., Liang Y., Shi H., Gao L., Zhan S., Zhou D., Luo Y. yluo@tsinghua.edu.cn 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Fu Y., Chen Y., Luo X., Liang Y., Shi H., Gao L., Zhan S., Zhou D., Luo Y. yluo@tsinghua.edu.cn Accepted 2012-JUL-30 by ORCHARD 19-Sept-2012: Contacted by IntAct-Help. Biochemistry (0006-2960) 2009 Only protein-protein interactions imex curation p39060-pro_0000005794 Endostatin COL18A1 protein protein human Homo sapiens Human HSHRDFQPVLHLVALNSPLSGGMRGIRGADFQCFQQARAVGLAGTFRAFLSSRLQDLYSIVRRADRAAVPIVNLKDELLFPSWEALFSGSEGPLKPGARIFSFDGKDVLRHPTWPQKSVWHGSDPNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSAASCHHAYIVLCIENSFMTASK 1572 1754 639F583202516B31 nucl_human Nucleolin NCL Protein C23 protein protein human Homo sapiens Human MVKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQKKGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAKAVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDDDDDEEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEEDDEDEDDDDDEDDEDDDDEDDEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEGTEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLPYKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNSTWSGESKTLVLSNLSYSATEETLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE DIP protein P19338 original sequence version: 93 DIP protein P19338 original sequence version: 123 C97F6E34E5CA6727 heparin Heparin small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ncl-col18a1 3 5 unspecified role unspecified role prey prey native native tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag polyclonal western polyclonal antibody western blot n-term range n-terminal range n-term range n-term range n-terminal range n-term range P39060-PRO_0000005794:p.[Arg24Ala;Arg27Ala;Arg53Ala;Arg62_Arg63delinsAlaAla;Arg139Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain RR AA Thu Jul 07 12:07:26 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was arg24_27_53_62_63_139ala Fri Jul 22 11:14:15 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg24ala,arg27ala,arg53ala,arg62ala,arg63ala,arg139ala' Wed May 02 19:59:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg24ala,arg27ala,arg53ala,arg_arg62-63ala_ala,arg139ala' Tue Jun 30 18:41:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg24Ala;Arg27Ala;Arg53Ala;Arg62_Arg63delinsAlaAla;Arg139Ala]' P39060-PRO_0000005794:p.[Arg27Ala;Arg139Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:31:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg27ala,arg139ala' Tue Jun 30 18:49:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg27Ala;Arg139Ala]' P39060-PRO_0000005794:p.[Arg24Ala;Arg53Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:00:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg24ala,arg53ala' Tue Jun 30 15:37:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg24Ala;Arg53Ala]' P39060-PRO_0000005794:p.[Arg24Ala;Arg27Ala;Arg53Ala;Arg139Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:00:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg24ala,arg27ala,arg53ala,arg139ala' Tue Jun 30 15:48:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg24Ala;Arg27Ala;Arg53Ala;Arg139Ala]' P39060-PRO_0000005794:p.[Arg62Ala;Arg63Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:57:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg62ala,arg63ala' Tue Jun 30 20:08:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg62Ala;Arg63Ala]' ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait native native tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction 2A col18a1-ncl-1 1 5 unspecified role unspecified role neutral component neutral component tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component human-hmec-1 Human HMEC-1 immortalized microvascular endothelial cell line hmec-1 HMEC-1 - microvascular endothelial cell line physical association physical association 3A, 3B ncl-col18a1-2 3 5 unspecified role unspecified role prey prey tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag polyclonal western polyclonal antibody western blot n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 7 competitor competitor competitor neutral component neutral component direct interaction direct interaction Fig. 2A ncl-col18a1-3 4 5 unspecified role unspecified role prey prey P39060-PRO_0000005794:p.[Arg27Ala;Arg139Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:27:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg27ala,arg139ala' Tue Jun 30 19:38:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg27Ala;Arg139Ala]' P39060-PRO_0000005794:p.Arg62_Arg63delinsAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain RR AA Wed May 02 19:32:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg62-63ala_ala' Tue Jun 30 16:18:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg62_Arg63delinsAlaAla' arg24ala,arg27ala,arg53ala,arg139ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed Jul 06 17:06:28 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'ala24_27_53_139ala' P39060-PRO_0000005794:p.[Arg24Ala;Arg53Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 20:12:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg24ala,arg53ala' Tue Jun 30 19:27:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg24Ala;Arg53Ala]' P39060-PRO_0000005794:p.[Arg24Ala;Arg27Ala;Arg53Ala;Arg62Ala;Arg63Ala;Arg139Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Fri Jul 22 10:59:55 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg24ala,arg27ala,arg53ala,arg62ala,arg139ala,arg63ala' Wed May 02 19:23:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg24ala,arg27ala,arg53ala,arg62ala,arg63ala,arg139ala' Tue Jun 30 19:54:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg24Ala;Arg27Ala;Arg53Ala;Arg62Ala;Arg63Ala;Arg139Ala]' tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction 2B col18a1-ncl-8 2 6 unspecified role unspecified role neutral component neutral component tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 5 unspecified role unspecified role neutral component neutral component tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range P39060-PRO_0000005794:p.[Arg24Ala;Arg53Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Fri Jul 22 10:59:41 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg53ala,arg24ala' Wed May 02 15:18:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg24ala,arg53ala' Tue Jun 30 18:13:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg24Ala;Arg53Ala]' P39060-PRO_0000005794:p.Arg62_Arg63delinsAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain RR AA Wed May 02 17:51:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg62-63ala_ala' Tue Jun 30 16:24:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg62_Arg63delinsAlaAla' P39060-PRO_0000005794:p.[Arg27Ala;Arg139Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 20:29:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg27ala,arg139ala' Tue Jun 30 19:48:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg27Ala;Arg139Ala]' P39060-PRO_0000005794:p.[Arg24Ala;Arg27Ala;Arg53Ala;Arg139Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:50:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg24ala,arg27ala,arg53ala,arg139ala' Tue Jun 30 16:50:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg24Ala;Arg27Ala;Arg53Ala;Arg139Ala]' P39060-PRO_0000005794:p.[Arg24Ala;Arg27Ala;Arg53Ala;Arg62Ala;Arg63Ala;Arg139Ala] mutation disrupting mutation disrupting interaction mutation disrupting predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:08:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg24ala,arg27ala,arg53ala,arg62ala,arg63ala,arg139ala' Tue Jun 30 18:13:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg24Ala;Arg27Ala;Arg53Ala;Arg62Ala;Arg63Ala;Arg139Ala]' ecoli Escherichia coli (strain K12) colocalization colocalization 5D