IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ CIP2A inhibits PP2A in human malignancies. CIP2A inhibits PP2A in human malignancies. Cell (0092-8674) 2007 imex curation Junttila MR., Puustinen P., Niemela M., Ahola R., Arnold H., Bottzauw T., Ala-aho R., Nielsen C., Ivaska J., Taya Y., Lu SL., Lin S., Chan EK., Wang XJ., Grenman R., Kast J., Kallunki T., Sears R., Kahari VM., Westermarck J. Cancer - Interactions investigated in the context of cancer Source of all protein constructs supplied by the authors. ltjuwe@uta.fi 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions 6 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Junttila MR., Puustinen P., Niemela M., Ahola R., Arnold H., Bottzauw T., Ala-aho R., Nielsen C., Ivaska J., Taya Y., Lu SL., Lin S., Chan EK., Wang XJ., Grenman R., Kast J., Kallunki T., Sears R., Kahari VM., Westermarck J. Cancer - Interactions investigated in the context of cancer Source of all protein constructs supplied by the authors. ltjuwe@uta.fi Accepted 2008-SEP-19 by DAVET. 27-Aug-2008: Contacted by JYOTI. Cell (0092-8674) 2007 davet Only protein-protein interactions 6 imex curation CIP2A inhibits PP2A in human malignancies. CIP2A inhibits PP2A in human malignancies. Cell (0092-8674) 2007 imex curation Junttila MR., Puustinen P., Niemela M., Ahola R., Arnold H., Bottzauw T., Ala-aho R., Nielsen C., Ivaska J., Taya Y., Lu SL., Lin S., Chan EK., Wang XJ., Grenman R., Kast J., Kallunki T., Sears R., Kahari VM., Westermarck J. Cancer - Interactions investigated in the context of cancer Source of all protein constructs supplied by the authors. ltjuwe@uta.fi 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions 6 human-ht1080 Homo sapiens HT1080 fribrosarcoma cell line ht1080 confirmed as human with IEF of AST, LDH, NP established from the biopsy from a fibrosarcoma of a 35-year-old Caucasian man in 1972 anti tag coip anti tag coimmunoprecipitation anti tag coip de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Junttila MR., Puustinen P., Niemela M., Ahola R., Arnold H., Bottzauw T., Ala-aho R., Nielsen C., Ivaska J., Taya Y., Lu SL., Lin S., Chan EK., Wang XJ., Grenman R., Kast J., Kallunki T., Sears R., Kahari VM., Westermarck J. Cancer - Interactions investigated in the context of cancer Source of all protein constructs supplied by the authors. ltjuwe@uta.fi Accepted 2008-SEP-19 by DAVET. Cell (0092-8674) 2007 davet Only protein-protein interactions 6 imex curation CIP2A inhibits PP2A in human malignancies. CIP2A inhibits PP2A in human malignancies. Cell (0092-8674) 2007 imex curation Junttila MR., Puustinen P., Niemela M., Ahola R., Arnold H., Bottzauw T., Ala-aho R., Nielsen C., Ivaska J., Taya Y., Lu SL., Lin S., Chan EK., Wang XJ., Grenman R., Kast J., Kallunki T., Sears R., Kahari VM., Westermarck J. Cancer - Interactions investigated in the context of cancer Source of all protein constructs supplied by the authors. ltjuwe@uta.fi 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions 6 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Junttila MR., Puustinen P., Niemela M., Ahola R., Arnold H., Bottzauw T., Ala-aho R., Nielsen C., Ivaska J., Taya Y., Lu SL., Lin S., Chan EK., Wang XJ., Grenman R., Kast J., Kallunki T., Sears R., Kahari VM., Westermarck J. Cancer - Interactions investigated in the context of cancer Source of all protein constructs supplied by the authors. ltjuwe@uta.fi Accepted 2008-SEP-19 by DAVET. 27-Aug-2008: Contacted by JYOTI. Cell (0092-8674) 2007 davet Only protein-protein interactions 6 imex curation CIP2A inhibits PP2A in human malignancies. CIP2A inhibits PP2A in human malignancies. Cell (0092-8674) 2007 imex curation Junttila MR., Puustinen P., Niemela M., Ahola R., Arnold H., Bottzauw T., Ala-aho R., Nielsen C., Ivaska J., Taya Y., Lu SL., Lin S., Chan EK., Wang XJ., Grenman R., Kast J., Kallunki T., Sears R., Kahari VM., Westermarck J. Cancer - Interactions investigated in the context of cancer Source of all protein constructs supplied by the authors. ltjuwe@uta.fi 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions 6 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Junttila MR., Puustinen P., Niemela M., Ahola R., Arnold H., Bottzauw T., Ala-aho R., Nielsen C., Ivaska J., Taya Y., Lu SL., Lin S., Chan EK., Wang XJ., Grenman R., Kast J., Kallunki T., Sears R., Kahari VM., Westermarck J. Cancer - Interactions investigated in the context of cancer Source of all protein constructs supplied by the authors. ltjuwe@uta.fi Accepted 2008-SEP-19 by DAVET. 27-Aug-2008: Contacted by JYOTI. Cell (0092-8674) 2007 davet Only protein-protein interactions 6 imex curation CIP2A inhibits PP2A in human malignancies. CIP2A inhibits PP2A in human malignancies. Cell (0092-8674) 2007 imex curation Junttila MR., Puustinen P., Niemela M., Ahola R., Arnold H., Bottzauw T., Ala-aho R., Nielsen C., Ivaska J., Taya Y., Lu SL., Lin S., Chan EK., Wang XJ., Grenman R., Kast J., Kallunki T., Sears R., Kahari VM., Westermarck J. Cancer - Interactions investigated in the context of cancer Source of all protein constructs supplied by the authors. ltjuwe@uta.fi 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions 6 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Junttila MR., Puustinen P., Niemela M., Ahola R., Arnold H., Bottzauw T., Ala-aho R., Nielsen C., Ivaska J., Taya Y., Lu SL., Lin S., Chan EK., Wang XJ., Grenman R., Kast J., Kallunki T., Sears R., Kahari VM., Westermarck J. Cancer - Interactions investigated in the context of cancer Source of all protein constructs supplied by the authors. ltjuwe@uta.fi Accepted 2008-SEP-19 by DAVET. 27-Aug-2008: Contacted by JYOTI. Cell (0092-8674) 2007 davet Only protein-protein interactions 6 imex curation CIP2A inhibits PP2A in human malignancies. CIP2A inhibits PP2A in human malignancies. Cell (0092-8674) 2007 imex curation Junttila MR., Puustinen P., Niemela M., Ahola R., Arnold H., Bottzauw T., Ala-aho R., Nielsen C., Ivaska J., Taya Y., Lu SL., Lin S., Chan EK., Wang XJ., Grenman R., Kast J., Kallunki T., Sears R., Kahari VM., Westermarck J. Cancer - Interactions investigated in the context of cancer Source of all protein constructs supplied by the authors. ltjuwe@uta.fi 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions 6 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Junttila MR., Puustinen P., Niemela M., Ahola R., Arnold H., Bottzauw T., Ala-aho R., Nielsen C., Ivaska J., Taya Y., Lu SL., Lin S., Chan EK., Wang XJ., Grenman R., Kast J., Kallunki T., Sears R., Kahari VM., Westermarck J. Cancer - Interactions investigated in the context of cancer Source of all protein constructs supplied by the authors. ltjuwe@uta.fi Accepted 2008-SEP-19 by DAVET. 27-Aug-2008: Contacted by JYOTI. Cell (0092-8674) 2007 davet Only protein-protein interactions 6 imex curation cip2a_human Protein CIP2A CIP2A Cancerous inhibitor of PP2A KIAA1524 p90 autoantigen protein protein human Homo sapiens Human MDSTACLKSLLLTVSQYKAVKSEANATQLLRHLEVISGQKLTRLFTSNQILTSECLSCLVELLEDPNISASLILSIIGLLSQLAVDIETRDCLQNTYNLNSVLAGVVCRSSHTDSVFLQCIQLLQKLTYNVKIFYSGANIDELITFLIDHIQSSEDELKMPCLGLLANLCRHNLSVQTHIKTLSNVKSFYRTLITLLAHSSLTVVVFALSILSSLTLNEEVGEKLFHARNIHQTFQLIFNILINGDGTLTRKYSVDLLMDLLKNPKIADYLTRYEHFSSCLHQVLGLLNGKDPDSSSKVLELLLAFCSVTQLRHMLTQMMFEQSPPGSATLGSHTKCLEPTVALLRWLSQPLDGSENCSVLALELFKEIFEDVIDAANCSSADRFVTLLLPTILDQLQFTEQNLDEALTRKKCERIAKAIEVLLTLCGDDTLKMHIAKILTTVKCTTLIEQQFTYGKIDLGFGTKVADSELCKLAADVILKTLDLINKLKPLVPGMEVSFYKILQDPRLITPLAFALTSDNREQVQSGLRILLEAAPLPDFPALVLGESIAANNAYRQQETEHIPRKMPWQSSNHSFPTSIKCLTPHLKDGVPGLNIEELIEKLQSGMVVKDQICDVRISDIMDVYEMKLSTLASKESRLQDLLETKALALAQADRLIAQHRCQRTQAETEARTLASMLREVERKNEELSVLLKAQQVESERAQSDIEHLFQHNRKLESVAEEHEILTKSYMELLQRNESTEKKNKDLQITCDSLNKQIETVKKLNESLKEQNEKSIAQLIEKEEQRKEVQNQLVDREHKLANLHQKTKVQEEKIKTLQKEREDKEETIDILRKELSRTEQIRKELSIKASSLEVQKAQLEGRLEEKESLVKLQQEELNKHSHMIAMIHSLSGGKINPETVNLSI DAB9CCC03BCEA304 2aaa_human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform PPP2R1A PP2A subunit A isoform PR65-alpha PP2A subunit A isoform R1-alpha Medium tumor antigen-associated 61 kDa protein protein protein human Homo sapiens Human MAAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFTTLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA DIP protein P30153 original sequence version: 153 DIP protein P30153 original sequence version: 71 DIP protein P30153 original sequence version: 105 DIP protein P30153 original sequence version: 107 DIP protein P30153 original sequence version: 151 DIP protein P30153 original sequence version: 156 DIP protein P30153 original sequence version: 155 DIP protein P30153 original sequence version: 169 5174EBE94D537836 myc_human Myc proto-oncogene protein BHLHE39 Transcription factor p64 MYC Proto-oncogene c-Myc Class E basic helix-loop-helix protein 39 protein protein human Homo sapiens Human MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA DIP protein P01106 original sequence version: 195 DIP protein P01106 original sequence version: 196 DIP protein P01106 original sequence version: 148 DIP protein P01106-1 original sequence version: 161 DIP protein P01106 original sequence version: 182 DIP protein P01106 original sequence version: 197 DIP protein P01106-1 original sequence version: 208 DIP protein P01106 original sequence version: 121 DIP protein P01106-1 original sequence version: 144 DIP protein P01106 original sequence version: 144 ED5C028029A4C5D1 2aba_human Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform PPP2R2A PP2A subunit B isoform alpha PP2A subunit B isoform B55-alpha PP2A subunit B isoform PR55-alpha PP2A subunit B isoform R2-alpha protein protein human Homo sapiens Human MAGAGGGNDIQWCFSQVKGAVDDDVAEADIISTVEFNHSGELLATGDKGGRVVIFQQEQENKIQSHSRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQKNAAQFLLSTNDKTIKLWKISERDKRPEGYNLKEEDGRYRDPTTVTTLRVPVFRPMDLMVEASPRRIFANAHTYHINSISINSDYETYLSADDLRINLWHLEITDRSFNIVDIKPANMEELTEVITAAEFHPNSCNTFVYSSSKGTIRLCDMRASALCDRHSKLFEEPEDPSNRSFFSEIISSISDVKFSHSGRYMMTRDYLSVKIWDLNMENRPVETYQVHEYLRSKLCSLYENDCIFDKFECCWNGSDSVVMTGSYNNFFRMFDRNTKRDITLEASRENNKPRTVLKPRKVCASGKRKKDEISVDSLDFNKKILHTAWHPKENIIAVATTNNLYIFQDKVN DIP protein P63151 original sequence version: 33 DIP protein P63151 original sequence version: 66 F4D407FF7ADA4ED6 ppp2r1a-kiaa1524-1 2 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association A catalytic subunit of PP2a was also identified but not as a specific gene product. Fig 1 B and Supp Table 1. P30153 transfection. ppp2r1a-kiaa1524-2 6 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association A catalytic subunit of PP2a was also identified but not as a specific gene product. Fig 1 C Indeed, endogenous CIP2A and PP2Ac coimmunoprecipitated with the endogenous PR65 (Figure 1C). ppp2r1a-kiaa1524-3 4 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component physical association physical association Confocal microscopy of endogenous PR65 and CIP2A in HeLa cells Fig 1 D ppp2r1a-kiaa1524-4 5 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey physical association physical association Fig 1 E To strengthen the interaction data between CIP2A and the PP2A complex, a series of Flag-tagged CIP2A deletion constructs (Figure S1) were transiently transfected into HeLa cells for 48 hr, followed by immunoprecipitation with anti-Flag antibody. Q8TCG1 transfection myc-kiaa1524-1 3 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 9 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 3D In vitro binding assay with immobilized, recombinant Flag-CIP2A protein and recombinant GST protein or with GST-c-Myc 1-262 protein. myc-kiaa1524-2 1 7 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 9 unspecified role unspecified role bait bait P01106:p.Ser62Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S D Wed May 02 14:32:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser62asp' Tue Jun 30 18:58:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser62Asp' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P01106:p.Ser62Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:41:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser62ala' Tue Jun 30 19:28:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser62Ala' region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9bact Bacteria physical association physical association Fig 3E, Supp. Fig. 3 293 cells transiently cotransfected for 48 hr with V5/His-c-Myc, and Flag-CIP2A expression constructs were subjected to immunoprecipitation by anti-V5 antibody. P01106; Q8TCG1 transfection