IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Tumorigenic transformation by CPI-17 through inhibition of a merlin phosphatase. Tumorigenic transformation by CPI-17 through inhibition of a merlin phosphatase. Nature (0028-0836) 2006 imex curation Jin H., Sperka T., Herrlich P., Morrison H. BioCreative - Critical Assessment of Information Extraction systems in Biology Cancer - Interactions investigated in the context of cancer helen@fli-leibniz.de 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions rat-rt4_d6p2t Rattus norvegicus Schwann cell, immature rt4_d6p2t Rat Schwann cell, immature anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jin H., Sperka T., Herrlich P., Morrison H. helen@fli-leibniz.de Accepted 2006-SEP-15 by ORCHARD. Nature (0028-0836) 2006 Tumorigenic transformation by CPI-17 through inhibition of a merlin phosphatase. Tumorigenic transformation by CPI-17 through inhibition of a merlin phosphatase. Nature (0028-0836) 2006 imex curation Jin H., Sperka T., Herrlich P., Morrison H. BioCreative - Critical Assessment of Information Extraction systems in Biology Cancer - Interactions investigated in the context of cancer helen@fli-leibniz.de 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jin H., Sperka T., Herrlich P., Morrison H. BioCreative - Critical Assessment of Information Extraction systems in Biology helen@fli-leibniz.de Accepted 2006-SEP-15 by ORCHARD. 24-JAN-2007: Contacted by JYOTI. Nature (0028-0836) 2006 Only protein-protein interactions imex curation Tumorigenic transformation by CPI-17 through inhibition of a merlin phosphatase. Tumorigenic transformation by CPI-17 through inhibition of a merlin phosphatase. Nature (0028-0836) 2006 imex curation Jin H., Sperka T., Herrlich P., Morrison H. BioCreative - Critical Assessment of Information Extraction systems in Biology Cancer - Interactions investigated in the context of cancer helen@fli-leibniz.de 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions rat-rt4_d6p2t Rattus norvegicus Schwann cell, immature rt4_d6p2t Rat Schwann cell, immature anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Jin H., Sperka T., Herrlich P., Morrison H. BioCreative - Critical Assessment of Information Extraction systems in Biology helen@fli-leibniz.de Accepted 2006-SEP-15 by ORCHARD. 24-JAN-2007: Contacted by JYOTI. Nature (0028-0836) 2006 Only protein-protein interactions imex curation pp1b_mouse Serine/threonine-protein phosphatase PP1-beta catalytic subunit Ppp1cb protein protein mouse Mus musculus Mouse MADGELNVDSLITRLLEVRGCRPGKIVQMTEAEVRGLCIKSREIFLSQPILLELEAPLKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR E8356022E9B94ECD pp1b_rat Serine/threonine-protein phosphatase PP1-beta catalytic subunit Ppp1cb protein protein rat Rattus norvegicus (Rat) Rat MADGELNVDSLITRLLEVRGCRPGKIVQMTEAEVRGLCIKSREIFLSQPILLELEAPLKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR E8356022E9B94ECD merl_rat Merlin Moesin-ezrin-radixin-like protein Neurofibromin-2 Schwannomin Nf2 protein protein rat Rattus norvegicus (Rat) Rat IASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKVYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFTIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERSRDEPERRVLHMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFDIIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHSEHSDSGTSSKHNTIKKPQAQGRRPICI 2385AF8461407B24 merl_human Merlin Moesin-ezrin-radixin-like protein Neurofibromin-2 Schwannomin Schwannomerlin NF2 SCH protein protein human Homo sapiens Human MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKIYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFAIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNTIKKLTLQSAKSRVAFFEEL B1A1BF2BD5DA561C merl_mouse Merlin Moesin-ezrin-radixin-like protein Neurofibromin-2 Schwannomin Nf2 Nf-2 protein protein mouse Mus musculus Mouse MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKVYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFTIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPPPLPPDIPSFDIIADSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDVLHSESSDRGGPSSKHNTIKKLTLQSAKSRVAFFEEL 8D06F557E3435851 mypt1_rat Protein phosphatase 1 regulatory subunit 12A Ppp1r12a Mbs Mypt1 Myosin phosphatase-targeting subunit 1 Protein phosphatase myosin-binding subunit MBSP Serine/threonine protein phosphatase PP1 smooth muscle regulatory subunit M110 Protein phosphatase subunit 1M protein protein rat Rattus norvegicus (Rat) Rat MKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYANVDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHISDVRHAKSGGTALHVAAAKGYTEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMETVNKVGQTAFDVADEDILGYLEELQKKQNLLHSEKRDKKSPLIESTANMENNQPQKTFKNKETLIIEPEKNASRIESLEQEKADEEEEGKKDESSCSSEEDEEDDSESEAETDKTKPMASVTNAHTASTQAAPAAVTTPTLSSNQGTPTSPVKKFPTSTTKISPKEEERKDESPASWRLGLRKTGSYGALAEITASKEAQKEKDTAGVIRSASSPRLSSSLDNKEKEKDNKGTRLAYVAPTIPRRLGSTSDIEEKENRESSNLRTSSSYTRRKWEDDLKKNSSINEGSTYHRSCSFGRRQDDLISCSVPSTTSTPTVTSAAGLQKSFLSSTSTTAKTPPGSSPAGTQSSTSNRLWAEDSTEKEKDSAPTAATILVAPTVVSAAASSTTALTTTTAGTLSSTSEVRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKDKEEKEKQDKEKQEEKKESEVSREDEYKQKYSRTYDETYARYRPVSTSSSSTPSSSSLSTLGSSLYASSQLNRPNSLVGITSAYSRGLTKDNEREGEKKEEEKEGEDKSQPKSIRERRRPREKRRSTGVSFWTQDSDENEQERQSDTEDGSSKRDTQTDSVSRYDSSSTSSSDRYDSLLGRSASYSYLEERKPYGSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKATQRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK 43B9FC1569CD5DDB mypt1_mouse Protein phosphatase 1 regulatory subunit 12A Myosin phosphatase-targeting subunit 1 Ppp1r12a Mypt1 protein protein mouse Mus musculus Mouse MKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYANVDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERVMLRDARQWLNSGHISDVRHAKSGGTALHVAAAKGYTEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMETVNKVGQTAFDVADEDILGYLEELQKKQTLLHSEKRDKKSPLIESTANMENNQPQKAFKNKETLIIEPEKNASRIESLEHEKADEEEEGKKDESSCSSEEDEEDDSESEAETDKTKPMASVSNAHTSSTQAAPAAVTAPTLSSNQGTPTSPVKKFPISTTKISPKEEERKDESPASWRLGLRKTGSYGALAEISASKEAQKEKDTAGVMRSASSPRLSSSLDNKEKEKDNKGTRLAYVTPTIPRRLASTSDIEEKENRESSSLRTSSSYTRRKWEDDLKKNSSINEGSTYHRSCSFGRRQDDLISCSVPSTTSTPTVTSAAGLQRSLPSSTSTAAKTPPGSSSAGTQSSTSNRLWAEDSTEKEKDSAPTAVTIPVAPTVVNAAAPSTTTLTTTTAGTVSEVRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKEKEEKEKQDKEKQEEKKESEASREDEYKQKYSRTYDETYTRYRPVSTSSSSAPSSSSLSTLGSTLYASSQLNRPNSLVGITSAYSRGLAKENEREGEKKEEEKEGEDKSQPKSIRERRRPREKRRSTGVSFWTQDSDENEQERQSDTEDGSSKRETQTDSVSRYDSSSTSSSDRYDSLLGRSASYSYLEDRKPYSSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKATQRQERFADRSQLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK DIP protein Q9DBR7 original sequence version: 54 C466573AC1DFC81F nf2-ppp1cb 1 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey association association PP1d and MYPT-1 were co-precipitated with endogenous merlin from lysates of both RT4 and NIH 3T3 cells 2A The PP1 delta catalytic subunit is also named the beta catalytic subunit. nf2-ppm1d 2 8 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey association association 2 A PP1d and MYPT-1 were co-precipitated with endogenous merlin from lysates of both RT4 and NIH 3T3 cells The PP1 delta catalytic subunit referred to by the authors is also called the beta catalytic subunit. nf2-ppp1r12a 3 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association S1 The Coomassie-stained gel also revealed a band at a molecular mass of about 130 kDa, which by immunoblot was identified as MYPT-1 Transfection These RTtet-C-merlin cells were engineered to have Dox-inducible merlin expression. ppp1r12a-nf2 1 7 unspecified role unspecified role prey prey P35240:p.Ser518Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:44:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser518ala' Tue Jun 30 20:18:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser518Ala' 9 unspecified role unspecified role bait bait physical association physical association MYPT-1 preferred the phosphorylated form of merlin, because fulllength S518D or wild type merlin compared to S518A merlin coprecipitated with MYPT-1 2 C MYPT-1 preferred the phosphorylated form of merlin as it coprecipitated in greated quantities with the wild-type merlin of ser518asp mutant than the ser518ala non-phosphorylatable mutant merlin.