IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Nck adaptor proteins link nephrin to the actin cytoskeleton of kidney podocytes. Nck adaptor proteins link nephrin to the actin cytoskeleton of kidney podocytes. Nature (0028-0836) 2006 imex curation Jones N., Blasutig IM., Eremina V., Ruston JM., Bladt F., Li H., Huang H., Larose L., Li SS., Takano T., Quaggin SE., Pawson T. BioCreative - Critical Assessment of Information Extraction systems in Biology pawson@mshri.on.ca 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Jones N., Blasutig IM., Eremina V., Ruston JM., Bladt F., Li H., Huang H., Larose L., Li SS., Takano T., Quaggin SE., Pawson T. BioCreative - Critical Assessment of Information Extraction systems in Biology pawson@mshri.on.ca Accepted 2006-SEP-14 by JYOTI. 24-JAN-2007: Contacted by JYOTI. Nature (0028-0836) 2006 Only protein-protein interactions imex curation Nck adaptor proteins link nephrin to the actin cytoskeleton of kidney podocytes. Nck adaptor proteins link nephrin to the actin cytoskeleton of kidney podocytes. Nature (0028-0836) 2006 imex curation Jones N., Blasutig IM., Eremina V., Ruston JM., Bladt F., Li H., Huang H., Larose L., Li SS., Takano T., Quaggin SE., Pawson T. BioCreative - Critical Assessment of Information Extraction systems in Biology pawson@mshri.on.ca 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Jones N., Blasutig IM., Eremina V., Ruston JM., Bladt F., Li H., Huang H., Larose L., Li SS., Takano T., Quaggin SE., Pawson T. BioCreative - Critical Assessment of Information Extraction systems in Biology pawson@mshri.on.ca Accepted 2006-SEP-14 by JYOTI. Nature (0028-0836) 2006 Only protein-protein interactions imex curation Nck adaptor proteins link nephrin to the actin cytoskeleton of kidney podocytes. Nck adaptor proteins link nephrin to the actin cytoskeleton of kidney podocytes. Nature (0028-0836) 2006 imex curation Jones N., Blasutig IM., Eremina V., Ruston JM., Bladt F., Li H., Huang H., Larose L., Li SS., Takano T., Quaggin SE., Pawson T. BioCreative - Critical Assessment of Information Extraction systems in Biology pawson@mshri.on.ca 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jones N., Blasutig IM., Eremina V., Ruston JM., Bladt F., Li H., Huang H., Larose L., Li SS., Takano T., Quaggin SE., Pawson T. BioCreative - Critical Assessment of Information Extraction systems in Biology It was not possible to identify the UniProt accession number for RAT Nck protein and data relevant to is protein was ommitted (Figure 2c). pawson@mshri.on.ca Accepted 2006-SEP-14 by JYOTI. 24-JAN-2007: Contacted by JYOTI. Nature (0028-0836) 2006 Only protein-protein interactions imex curation Nck adaptor proteins link nephrin to the actin cytoskeleton of kidney podocytes. Nck adaptor proteins link nephrin to the actin cytoskeleton of kidney podocytes. Nature (0028-0836) 2006 imex curation Jones N., Blasutig IM., Eremina V., Ruston JM., Bladt F., Li H., Huang H., Larose L., Li SS., Takano T., Quaggin SE., Pawson T. BioCreative - Critical Assessment of Information Extraction systems in Biology pawson@mshri.on.ca 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Jones N., Blasutig IM., Eremina V., Ruston JM., Bladt F., Li H., Huang H., Larose L., Li SS., Takano T., Quaggin SE., Pawson T. BioCreative - Critical Assessment of Information Extraction systems in Biology Mouse embryonic fibroblasts lacked endogenous Nck1 and Nck2 proteins. pawson@mshri.on.ca Accepted 2006-SEP-14 by JYOTI. 24-JAN-2007: Contacted by JYOTI. Nature (0028-0836) 2006 Only protein-protein interactions imex curation nck2_human Cytoplasmic protein NCK2 NCK adaptor protein 2 SH2/SH3 adaptor protein NCK-beta NCK2 GRB4 Growth factor receptor-bound protein 4 protein protein human Homo sapiens Human MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ 3A211085E46F4452 nphn_human Nephrin Renal glomerulus-specific cell adhesion receptor NPHS1 NPHN protein protein human Homo sapiens Human MALGTTLRASLLLLGLLTEGLAQLAIPASVPRGFWALPENLTVVEGASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPKLLLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRAGQSLELPCVARGGNPLATLQWLKNGQPVSTAWGTEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQTENKNVTLSCVSKSSRPRVLLRWWLGWRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKSLILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQLAVQFPPTNVTILANASALRPGDALNLTCVSVSSNPPVNLSWDKEGERLEGVAAPPRRAPFKGSAAARSVLLQVSSRDHGQRVTCRAHSAELRETVSSFYRLNVLYRPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSSGALHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDPTEVNVGGSVDIVCTVDANPILPGMFNWERLGEDEEDQSLDDMEKISRGPTGRLRIHHAKLAQAGAYQCIVDNGVAPPARRLLRLVVRFAPQVEHPTPLTKVAAAGDSTSSATLHCRARGVPNIVFTWTKNGVPLDLQDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNALGSDQTNIQLVSISRPDPPSGLKVVSLTPHSVGLEWKPGFDGGLPQRFCIRYEALGTPGFHYVDVVPPQATTFTLTGLQPSTRYRVWLLASNALGDSGLADKGTQLPITTPGLHQPSGEPEDQLPTEPPSGPSGLPLLPVLFALGGLLLLSNASCVGGVLWQRRLRRLAEGISEKTEAGSEEDRVRNEYEESQWTGERDTQSSTVSTTEAEPYYRSLRDFSPQLPPTQEEVSYSRGFTGEDEDMAFPGHLYDEVERTYPPSGAWGPLYDEVQMGPWDLHWPEDTYQDPRGIYDQVAGDLDTLEPDSLPFELRGHLV 7F5AFAF078BCF532 nck1_human Cytoplasmic protein NCK1 NCK1 NCK NCK adaptor protein 1 SH2/SH3 adaptor protein NCK-alpha protein protein human Homo sapiens Human MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDSASPADDSFVDPGERLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS 554E9B1A936AEF30 wasp_mouse Wiskott-Aldrich syndrome protein homolog Was Wasp protein protein mouse Mus musculus Mouse MNSGPGPVGGRPGGRGGPAVQQNIPSNLLQDHENQRLFELLGRKCWTLATTVVQLYLALPPGAEHWTMEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYLTPTPFFHTFAGDDCQVGLNFADESEAQAFRALVQEKIQKRNQRQSGERRQLPPPPAPINEERRGGLPPVPPHPGGDHGGPSGGPLSLGLVTVDIQNPDITSSRYRGLPAPGPGPTDKKRSGKKKISKADIGAPSGFKHVSHVGWDPQNGFDVNNLDPDLRSLFSRAGISEAQLTDAETSKLIYDFIEDQGGLEAVRQEMRRQEPLPPPPPPCRGGGGGGGGGGGGGGGGGGQPLRPPVVGSNKGRSGPLPPVPMGGAPPPPTPRGPPPPGRGGPPPPPPPATGRSGPPPPPLPGAGGPPAPPPPPPPPPPPPCPGSGPAPPPLPPTPVSGGSPAPGGGRGALLDQIRQGIQLNKTPGALENSVQQPPAQQSEGLVGALMHVMQKRSRVIHSSDEGEDQTGEDEEDDEWDD DIP protein P70315 original sequence version: 114 DIP protein P70315 original sequence version: 125 9C223733C59F0C8A nphs1-nck1 2 6 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind range range range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Figure 2a Identification of a phosphotyrosine-dependent interaction between nephrin and the Nck SH2/SH3 adaptor. The co-ordinates of the SH2 domain used in this study were not given and are taken from UniProt annotation of the protein. This interaction was only observed in pervandate stimulated or cells co-transfected with active Src (Proto-oncogene tyrosine-protein kinase). nck1-nphs1 3 6 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O60500:p.[Tyr1176Phe;Tyr1217Phe] try1176phe-try1217phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 15:05:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1176phe,tyr1217phe' Tue Jun 30 17:33:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr1176Phe;Tyr1217Phe]' O60500:p.[Tyr1193Phe;Tyr1217Phe] try1193phe-try1217phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 19:45:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1193phe,tyr1217phe' Tue Jun 30 17:12:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr1193Phe;Tyr1217Phe]' O60500:p.[Tyr1176Phe;Tyr1193Phe;Tyr1217Phe] try1176phe-try1193phe-try1217phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Fri Jul 22 10:59:59 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1217phe,tyr1193phe,tyr1176phe' Wed May 02 16:42:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1176phe,tyr1193phe,tyr1217phe' Tue Jun 30 16:06:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr1176Phe;Tyr1193Phe;Tyr1217Phe]' 7 unspecified role unspecified role prey prey physical association physical association Figure 2d Identification of a phosphotyrosine-dependent interaction between nephrin and the Nck SH2/SH3 adaptor. nck1-nphs1-1 3 6 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey physical association physical association pervandate Figure 2b Identification of a phosphotyrosine-dependent interaction between nephrin and the Nck SH2/SH3 adaptor. This interaction was only observed in pervandate stimulated or cells co-transfected with active Src (Proto-oncogene tyrosine-protein kinase). nphs1-nck2 4 5 unspecified role unspecified role prey prey c terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role bait bait region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c terminal Intracellular domain sufficient to bind sufficient binding region sufficient to bind range range range range region Extracellular and transmembrane domain of CD16 and CD7 proteins tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O60500:p.[Tyr1176Phe;Tyr1193Phe;Tyr1217Phe] try1176phe-try1193phe-try1217phe mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Fri Jul 22 10:55:08 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1176phe,tyr1217phe,tyr1193phe' Wed May 02 15:41:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1176phe,tyr1193phe,tyr1217phe' Tue Jun 30 16:54:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr1176Phe;Tyr1193Phe;Tyr1217Phe]' physical association physical association Figure 4a Nck recruitment to phosphorylated nephrin facilitates localised actin reorganisation. The nephrin protein was phosphorylated and tagged with CD16 and CD7 extracellular and transmembrane domains. Interaction between these proteins was increased when anti-DD16 antibody was used in the assay. The coordinates of the intracellular region of the nephrin protein were not given in the text and were determined from the annotation available in the corresponding UniProt accession entry. nphs1-wasp 4 8 unspecified role unspecified role prey prey c terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role bait bait region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c terminal Intracellular domain sufficient to bind sufficient binding region sufficient to bind range range range range region Extracellular and transmembrane domain of CD16 and CD7 proteins tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O60500:p.[Tyr1176Phe;Tyr1193Phe;Tyr1217Phe] try1176phe-try1193phe-try1217phe mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Fri Jul 22 11:10:21 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1217phe,tyr1193phe,tyr1176phe' Wed May 02 14:27:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1176phe,tyr1193phe,tyr1217phe' Tue Jun 30 16:45:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr1176Phe;Tyr1193Phe;Tyr1217Phe]' physical association physical association Figure 4a Nck recruitment to phosphorylated nephrin facilitates localised actin reorganisation. The nephrin protein was phosphorylated and tagged with CD16 and CD7 extracellular and transmembrane domains. Interaction between these proteins was increased when anti-DD16 antibody was used in the assay. The coordinates of the intracellular region of the nephrin protein were not given in the text and were determined from the annotation available in the corresponding UniProt accession entry. nphs1-nck2-1 1 5 unspecified role unspecified role neutral component neutral component c terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role neutral component neutral component region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c terminal Intracellular domain sufficient to bind sufficient binding region sufficient to bind range range range range region Extracellular and transmembrane domain of CD16 and CD7 proteins tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O60500:p.[Tyr1176Phe;Tyr1193Phe;Tyr1217Phe] try1176phe-try1193phe-try1217phe mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Fri Jul 22 10:58:52 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1176phe,tyr1217phe,tyr1193phe' Wed May 02 14:30:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1176phe,tyr1193phe,tyr1217phe' Tue Jun 30 20:19:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr1176Phe;Tyr1193Phe;Tyr1217Phe]' colocalization colocalization Figure 4c Figure 4d Nck recruitment to phosphorylated nephrin facilitates localised actin reorganisation. The nephrin protein was phosphorylated and tagged with CD16 and CD7 extracellular and transmembrane domains. The coordinates of the intracellular region of the nephrin protein were not given in the text and were determined from the annotation available in the corresponding UniProt accession entry.