IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Schizosaccharomyces pombe replication inhibitor Spd1 regulates ribonucleotide reductase activity and dNTPs by binding to the large Cdc22 subunit. The Schizosaccharomyces pombe replication inhibitor Spd1 regulates ribonucleotide reductase activity and dNTPs by binding to the large Cdc22 subunit. J. Biol. Chem. (0021-9258) 2006 imex curation Hakansson P., Dahl L., Chilkova O., Domkin V., Thelander L. BioCreative - Critical Assessment of Information Extraction systems in Biology pelle.hakanson@medchem.umu.se 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hakansson P., Dahl L., Chilkova O., Domkin V., Thelander L. BioCreative - Critical Assessment of Information Extraction systems in Biology pelle.hakanson@medchem.umu.se Accepted 2006-AUG-4 by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation rir1_yeast Ribonucleoside-diphosphate reductase large chain 1 RNR1 RIR1 Ribonucleotide reductase R1 subunit 1 YER070W CRT7 SDS12 Ribonucleotide reductase large subunit 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MYVYKRDGRKEPVQFDKITARISRLCYGLDPKHIDAVKVTQRIISGVYEGVTTIELDNLAAETCAYMTTVHPDYATLAARIAISNLHKQTTKQFSKVVEDLYRYVNAATGKPAPMISDDVYNIVMENKDKLNSAIVYDRDFQYSYFGFKTLERSYLLRINGQVAERPQHLIMRVALGIHGRDIEAALETYNLMSLKYFTHASPTLFNAGTPKPQMSSCFLVAMKEDSIEGIYDTLKECALISKTAGGIGLHIHNIRSTGSYIAGTNGTSNGLIPMIRVFNNTARYVDQGGNKRPGAFALYLEPWHADIFDFIDIRKNHGKEEIRARDLFPALWIPDLFMKRVEENGTWTLFSPTSAPGLSDCYGDEFEALYTRYEKEGRGKTIKAQKLWYSILEAQTETGTPFVVYKDACNRKSNQKNLGVIKSSNLCCEIVEYSAPDETAVCNLASVALPAFIETSEDGKTSTYNFKKLHEIAKVVTRNLNRVIDRNYYPVEEARKSNMRHRPIALGVQGLADTFMLLRLPFDSEEARLLNIQIFETIYHASMEASCELAQKDGPYETFQGSPASQGILQFDMWDQKPYGMWDWDTLRKDIMKHGVRNSLTMAPMPTASTSQILGYNECFEPVTSNMYSRRVLSGEFQVVNPYLLRDLVDLGIWDEGMKQYLITQNGSIQGLPNVPQELKDLYKTVWEISQKTIINMAADRSVYIDQSHSLNLFLRAPTMGKLTSMHFYGWKKGLKTGMYYLRTQAASAAIQFTIDQKIADQATENVADISNLKRPSYMPSSASYAASDFVPAAVTANATIPSLDSSSEASREASPAPTGSHSLTKGMAELNVQESKVEVPEVPAPTKNEEKAAPIVDDEETEFDIYNSKVIACAIDNPEACEMCSG 56BE1B077916E419 56BE1B077916E419 rir1_schpo Ribonucleoside-diphosphate reductase large chain cdc22 SPAC1F7.05 Ribonucleotide reductase protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MFVYKRDGRQEKVAFDKITARVSRLCYGLDSDHVDPVEITQKVISGVYPGVTTIELDNLAAETAATMTTKHPDYAILAARIAVSNLHKQTEKVFSTVVQQLHDYVNPKTDKPAPMISDKIYDIVMKHKDELDSAIIYDRDFTYNFFGFKTLERSYLLRIDGKVAERPQHMIMRVAVGIHGEDIEAAIETYNLMSQRYFTHASPTLFNAGTPRPQLSSCFLVTMKDDSIEGIYDTLKMCAMISKTAGGIGINIHNIRATGSYIAGTNGTSNGIVPMIRVYNNTARYVDQGGNKRPGAFAAYLEPWHADVMDFLELRKTHGNEDFRAREMFYALWIPDLFMQRVERNEQWTFFCPNEAPGLADVWGDEFVALYEKYEKENRGRRSLPAQKVWYAILQSQVETGNPFMLYKDSCNRKSNQKNVGTIRCSNLCTEIVEYSSPDEVAVCNLASVALPTFIKDGKYNFQKLHDVVKVVTRNLNKIIDVNYYPVPEARRSNMRHRPVGLGVQGLADAFFALRLPFESAGAKKLNIQIFETIYHAALEASCEIAQVEGTYESYEGSPASQGILQYDMWNVNPTDLWDWAELKEKIAKHGIRNSLLVAPMPTASTSQILGFNECFEPYTSNMYQRRVLSGEFQIVNPWLLKDLVERDLWNEDMKNKLVMLDGSIQAIPEIPQDLKDLYKTVWEISQKTVIDYAADRGPFIDQSQSLNIHLKDPSYGKITSMHFYGWKKGLKTGMYYLRTMAASAAIKFTVDPVALRARNEESNEENKKPVIKNGKAEISAEPTKEEIDIYNEKVLACSIKNPEACEMCSA 6650E0EE4C2E8DF6 spd1_schpo S-phase delaying protein 1 spd1 SPAC29B12.03 Protein p14 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MHSSKRVMTTKTHVEQPESSMRPQLPESIQGSLMDVGMRVRKSISTGYKSKQTTFPAYNPPLYNTVSENIALKNTAFSYEPNGTKRPFEQAIPNYNWANPPQDFEEPEWLKPFDVVMEGTNERL F43A6C9D86F55F51 sml1_yeast Ribonucleotide reductase inhibitor protein SML1 SML1 YM9958.04 YML058W protein protein yeast Saccharomyces cerevisiae Baker's yeast MQNSQDYFYAQNRCQQQQAPSTLRTVTMAEFRRVPLPPMAEVPMLSTQNSMGSSASASASSLEMWEKDLEERLNSIDHDMNNNKFGSGELKSMFNQGKVEEMDF 02735C9178E431B2 02735C9178E431B2 spd1-cdc22-1 1 4 unspecified role unspecified role bait bait ecobb Escherichia coli (strain B / BL21) 3 unspecified role unspecified role prey prey ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Temperature = 20C, Spd1 bound to chip at a concentration of 200pg/mm exp2 4A sensorgram showing the interaction between immobilized Spd1 (200RU) and increasing concentrations of mobile phase Cdc22p sml1-rnr1-1 1 2 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait direct interaction direct interaction Temperature = 20C 4B As a comparison we immobilized S.cerevisiae Sml1p to a sensor chip (Fig. 4B). Injection of S. cerevisiae Rnr1p resulted in an efficient binding