IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cooperation of ERK and SCFSkp2 for MKP-1 destruction provides a positive feedback regulation of proliferating signaling. Cooperation of ERK and SCFSkp2 for MKP-1 destruction provides a positive feedback regulation of proliferating signaling. J. Biol. Chem. (0021-9258) 2006 imex curation Lin YW., Yang JL. BioCreative - Critical Assessment of Information Extraction systems in Biology Further details of MAPK1 taken from vendors website. GST tags removed by thrombin cleavage prior to protein acting as prey lyang@life.nthu.edu.tw 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Lin YW., Yang JL. BioCreative - Critical Assessment of Information Extraction systems in Biology Further details of MAPK1 taken from vendors website. GST tags removed by thrombin cleavage prior to protein acting as prey lyang@life.nthu.edu.tw Accepted 2006-AUG-4 by JYOTI. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Cooperation of ERK and SCFSkp2 for MKP-1 destruction provides a positive feedback regulation of proliferating signaling. Cooperation of ERK and SCFSkp2 for MKP-1 destruction provides a positive feedback regulation of proliferating signaling. J. Biol. Chem. (0021-9258) 2006 imex curation Lin YW., Yang JL. BioCreative - Critical Assessment of Information Extraction systems in Biology Further details of MAPK1 taken from vendors website. GST tags removed by thrombin cleavage prior to protein acting as prey lyang@life.nthu.edu.tw 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Lin YW., Yang JL. BioCreative - Critical Assessment of Information Extraction systems in Biology Further details of MAPK1 taken from vendors website. GST tags removed by thrombin cleavage prior to protein acting as prey lyang@life.nthu.edu.tw Accepted 2006-AUG-4 by JYOTI. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Cooperation of ERK and SCFSkp2 for MKP-1 destruction provides a positive feedback regulation of proliferating signaling. Cooperation of ERK and SCFSkp2 for MKP-1 destruction provides a positive feedback regulation of proliferating signaling. J. Biol. Chem. (0021-9258) 2006 imex curation Lin YW., Yang JL. BioCreative - Critical Assessment of Information Extraction systems in Biology Further details of MAPK1 taken from vendors website. GST tags removed by thrombin cleavage prior to protein acting as prey lyang@life.nthu.edu.tw 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Lin YW., Yang JL. BioCreative - Critical Assessment of Information Extraction systems in Biology Further details of MAPK1 taken from vendors website. GST tags removed by thrombin cleavage prior to protein acting as prey lyang@life.nthu.edu.tw Accepted 2006-AUG-4 by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Cooperation of ERK and SCFSkp2 for MKP-1 destruction provides a positive feedback regulation of proliferating signaling. Cooperation of ERK and SCFSkp2 for MKP-1 destruction provides a positive feedback regulation of proliferating signaling. J. Biol. Chem. (0021-9258) 2006 imex curation Lin YW., Yang JL. BioCreative - Critical Assessment of Information Extraction systems in Biology Further details of MAPK1 taken from vendors website. GST tags removed by thrombin cleavage prior to protein acting as prey lyang@life.nthu.edu.tw 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lin YW., Yang JL. BioCreative - Critical Assessment of Information Extraction systems in Biology Further details of MAPK1 taken from vendors website. GST tags removed by thrombin cleavage prior to protein acting as prey lyang@life.nthu.edu.tw Accepted 2006-AUG-4 by JYOTI. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation dus1_human Dual specificity protein phosphatase 1 Mitogen-activated protein kinase phosphatase 1 Protein-tyrosine phosphatase CL100 Dual specificity protein phosphatase hVH1 DUSP1 CL100 MKP1 PTPN10 VH1 protein protein human Homo sapiens Human MVMEVGTLDAGGLRALLGERAAQCLLLDCRSFFAFNAGHIAGSVNVRFSTIVRRRAKGAMGLEHIVPNAELRGRLLAGAYHAVVLLDERSAALDGAKRDGTLALAAGALCREARAAQVFFLKGGYEAFSASCPELCSKQSTPMGLSLPLSTSVPDSAESGCSSCSTPLYDQGGPVEILPFLYLGSAYHASRKDMLDALGITALINVSANCPNHFEGHYQYKSIPVEDNHKADISSWFNEAIDFIDSIKNAGGRVFVHCQAGISRSATICLAYLMRTNRVKLDEAFEFVKQRRSIISPNFSFMGQLLQFESQVLAPHCSAEAGSPAMAVLDRGTSTTTVFNFPVSIPVHSTNSALSYLQSPITTSPSC DIP protein P28562 original sequence version: null 11BD1D39A9FCD51F skp2_human S-phase kinase-associated protein 2 F-box protein Skp2 Cyclin-A/CDK2-associated protein p45 p45skp2 F-box/LRR-repeat protein 1 SKP2 FBXL1 protein protein human Homo sapiens Human MHRKHLQEIPDLSSNVATSFTWGWDSSKTSELLSGMGVSALEKEEPDSENIPQELLSNLGHPESPPRKRLKSKGSDKDFVIVRRPKLNRENFPGVSWDSLPDELLLGIFSCLCLPELLKVSGVCKRWYRLASDESLWQTLDLTGKNLHPDVTGRLLSQGVIAFRCPRSFMDQPLAEHFSPFRVQHMDLSNSVIEVSTLHGILSQCSKLQNLSLEGLRLSDPIVNTLAKNSNLVRLNLSGCSGFSEFALQTLLSSCSRLDELNLSWCFDFTEKHVQVAVAHVSETITQLNLSGYRKNLQKSDLSTLVRRCPNLVHLDLSDSVMLKNDCFQEFFQLNYLQHLSLSRCYDIIPETLLELGEIPTLKTLQVFGIVPDGTLQLLKEALPHLQINCSHFTTIARPTIGNKKNQEIWGIKCRLTLQKPSCL F29B7C338A7A37E9 uba1_rabit Ubiquitin-like modifier-activating enzyme 1 Ubiquitin-activating enzyme E1 UBA1 UBE1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLPQVPSAPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNSPLEDQLRVGEFCHSRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSTMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMTELNGNQPIEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLSASLAEPDFVMTDFAKFSRPAQLHIGFQALHKFCAQHSRPPRPRNEEDAAELVTLARAVNSKASSAVQQDSLDEDLIRNLAFVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKESLTEDKCLPRQNRYDGQVAVFGSDLQEKLGRQKYFLVGAGAIGCELLKNFAMIGLGCGENGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVHQMNPHIRVTSHQNRVGPDTERIYDDDFFQTLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQLPQSWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVSNPLHLDYVMAAANLFAQTYGLAGSQDRAAVATLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRHLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR 1196EAC5EEEC06A5 mk01_human Mitogen-activated protein kinase 1 MAPK1 ERK2 PRKM1 Extracellular signal-regulated kinase 2 Mitogen-activated protein kinase 2 MAP kinase isoform p42 ERT1 PRKM2 protein protein human Homo sapiens Human MAAAAAAGAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS DIP protein P28482 original sequence version: 173 DIP protein P28482 original sequence version: 103 DIP protein P28482 original sequence version: 101 DIP protein P28482 original sequence version: 131 DIP protein P28482 original sequence version: 156 DIP protein P28482 original sequence version: 166 DIP protein P28482 original sequence version: 144 E85D0B2A5D2D724E ubb_human Polyubiquitin-B UBB protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGC 33011162F1C48BB1 ub2r1_human Ubiquitin-conjugating enzyme E2 R1 Ubiquitin-protein ligase R1 Ubiquitin-conjugating enzyme E2-32 kDa complementing Ubiquitin-conjugating enzyme E2-CDC34 CDC34 UBE2R1 UBCH3 (E3-independent) E2 ubiquitin-conjugating enzyme R1 E2 ubiquitin-conjugating enzyme R1 protein protein human Homo sapiens Human MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES DIP protein P49427 original sequence version: 101 DIP protein P49427 original sequence version: 134 258960666B589DB3 cul1_human Cullin-1 CUL1 protein protein human Homo sapiens Human MSSTRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAALNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA DIP protein Q13616 original sequence version: 144 DIP protein Q13616 original sequence version: 133 DIP protein Q13616 original sequence version: null DIP protein Q13616 original sequence version: 135 DIP protein Q13616 original sequence version: 98 DIP protein Q13616 original sequence version: 110 DIP protein Q13616 original sequence version: 146 DIP protein Q13616 original sequence version: 148 DIP protein Q13616 original sequence version: 100 DIP protein Q13616 original sequence version: 85 DIP protein Q13616 original sequence version: 114 DIP protein Q13616 original sequence version: 119 DIP protein Q13616 original sequence version: 136 DIP protein Q13616 original sequence version: 117 DIP protein Q13616 original sequence version: 99 DIP protein Q13616 original sequence version: 108 6625A1FFA7799BBA cks1_human Cyclin-dependent kinases regulatory subunit 1 PNAS-16 PNAS-143 CKS1B CKS1 protein protein human Homo sapiens Human MSHKQIYYSDKYDDEEFEYRHVMLPKDIAKLVPKTHLMSESEWRNLGVQQSQGWVHYMIHEPEPHILLFRRPLPKKPKK E64207B140FA38D5 mapk1-dusp1-1 3 8 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P28562:p.[Phe339Ala;Phe341Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A Fri Jul 22 11:06:01 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'phe341ala,phe339ala' Wed May 02 17:03:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe339ala,phe341ala' Tue Jun 30 17:42:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe339Ala;Phe341Ala]' ecoli Escherichia coli (strain K12) physical association physical association 2A, 1A Fig. 2A shows that N-MKP-1 formed a complex with active ERK2, but N-MKP- 1(ANAP) did not; conversely, neither N-MKP-1 nor N-MKP- 1(ANAP) associated with unactive ERK2. Interaction requires MAPK1 (ERK2) to be in active state - details of activation not given mapk1-dusp1-2 3 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P28562:p.[Phe339Ala;Phe341Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A Fri Jul 22 11:01:15 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'phe341ala,phe339ala' Wed May 02 17:05:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe339ala,phe341ala' Tue Jun 30 19:25:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe339Ala;Phe341Ala]' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) physical association physical association 1A Fig. 2A shows that N-MKP-1 formed a complex with active ERK2, but N-MKP- 1(ANAP) did not; conversely, neither N-MKP-1 nor N-MKP- 1(ANAP) associated with unactive ERK2. Interaction requires MAPK1 (ERK2) to be in active state - details of activation not given mapk1-dusp1-3 2 5 enzyme target enzyme target substrate substrate unspecified role unspecified role P28562:p.[Phe339Ala;Phe341Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A Fri Jul 22 11:01:52 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'phe341ala,phe339ala' Wed May 02 16:09:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe339ala,phe341ala' Tue Jun 30 17:26:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe339Ala;Phe341Ala]' region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 enzyme enzyme unspecified role unspecified role n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 2B & 4 Furthermore, active ERK2 could phosphorylate N-MKP-1 but not N-MKP-1(ANAP) (Fig. 2B). Phosphorylation of DUSP1 at Ser-296 and Ser-323 dusp1-ubiq-1 4 5 unspecified role unspecified role prey prey P28562:p.[Phe339Ala;Phe341Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A Fri Jul 22 11:12:52 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'phe341ala,phe339ala' Wed May 02 17:33:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe339ala,phe341ala' Tue Jun 30 18:07:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe339Ala;Phe341Ala]' region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P28562:p.Ser296Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:54:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser296ala' Tue Jun 30 17:15:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser296Ala' P28562:p.Ser323Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:34:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser323ala' Tue Jun 30 18:17:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser323Ala' 9 unspecified role unspecified role bait bait ubiquitination ubiquitination reaction ubiquitination The DEF motif is involved in ERK-directed MKP-1 degradation via the ubiquitin-proteasome pathway in vivo, whereas the N-terminal domain is not essential. 3A & 5A dusp1-skp2-1 4 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey P28562:p.Ser296Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:50:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser296ala' Tue Jun 30 19:07:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser296Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association As shown in Fig. 8A, endogenous Skp2 coexisted with MKP-1 containing phospho-Ser296 in an immunocomplex derived from H293 cells during forced expression of MKK1-CA or MKK2-CA; 8A Constitutively active MAP2K1 or MAP2K2 cotransfected in cells dusp1-skp2-2 4 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait P28562:p.Ser296Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:13:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser296ala' Tue Jun 30 20:05:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser296Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association The Skp2/ MKP-1 immunocomplex also contained the other SCF subunits Skp1 and Cul1 and the accessory protein Cks1 (Fig. 8A). 8A Constitutively active MAP2K1 or MAP2K2 cotransfected in cells dusp1-skp2-3 1 9 unspecified role unspecified role ancillary ancillary 6 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 7 unspecified role unspecified role ancillary ancillary 10 unspecified role unspecified role ancillary ancillary region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 enzyme target enzyme target substrate substrate unspecified role unspecified role P28562:p.Ser296Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:09:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser296ala' Tue Jun 30 16:58:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser296Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P28562:p.Ser323Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:48:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser323ala' Tue Jun 30 18:25:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser323Ala' region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association We next performed an in vitro assay to verify that the E3 activity of SCFSkp2 directs MKP-1 ubiquitination. 9 Phosphorylation of DUSP1 by MAPK1 on Ser-296 and Ser-323 was required for ubiquitination. Skp1, Cul1 and Cks1 were also present in the phosphorylation reaction.