IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification of the fibroblast growth factor (FGF)-interacting domain in a secreted FGF-binding protein by phage display. Identification of the fibroblast growth factor (FGF)-interacting domain in a secreted FGF-binding protein by phage display. J. Biol. Chem. (0021-9258) 2006 imex curation Xie B., Tassi E., Swift MR., McDonnell K., Bowden ET., Wang S., Ueda Y., Tomita Y., Riegel AT., Wellstein A. BioCreative - Critical Assessment of Information Extraction systems in Biology Biopanning experiment using N-terminal deletion mutants of FGFBP1 wellstea@georgetown.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro competition binding competition binding predetermined predetermined participant predetermined Xie B., Tassi E., Swift MR., McDonnell K., Bowden ET., Wang S., Ueda Y., Tomita Y., Riegel AT., Wellstein A. BioCreative - Critical Assessment of Information Extraction systems in Biology Biopanning experiment using N-terminal deletion mutants of FGFBP1 wellstea@georgetown.edu Accepted 2006-AUG-4 by JYOTI. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Identification of the fibroblast growth factor (FGF)-interacting domain in a secreted FGF-binding protein by phage display. Identification of the fibroblast growth factor (FGF)-interacting domain in a secreted FGF-binding protein by phage display. J. Biol. Chem. (0021-9258) 2006 imex curation Xie B., Tassi E., Swift MR., McDonnell K., Bowden ET., Wang S., Ueda Y., Tomita Y., Riegel AT., Wellstein A. BioCreative - Critical Assessment of Information Extraction systems in Biology Biopanning experiment using N-terminal deletion mutants of FGFBP1 wellstea@georgetown.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Xie B., Tassi E., Swift MR., McDonnell K., Bowden ET., Wang S., Ueda Y., Tomita Y., Riegel AT., Wellstein A. BioCreative - Critical Assessment of Information Extraction systems in Biology Biopanning experiment using N-terminal deletion mutants of FGFBP1 wellstea@georgetown.edu Accepted 2006-AUG-4 by JYOTI. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Identification of the fibroblast growth factor (FGF)-interacting domain in a secreted FGF-binding protein by phage display. Identification of the fibroblast growth factor (FGF)-interacting domain in a secreted FGF-binding protein by phage display. J. Biol. Chem. (0021-9258) 2006 imex curation Xie B., Tassi E., Swift MR., McDonnell K., Bowden ET., Wang S., Ueda Y., Tomita Y., Riegel AT., Wellstein A. BioCreative - Critical Assessment of Information Extraction systems in Biology Biopanning experiment using N-terminal deletion mutants of FGFBP1 wellstea@georgetown.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro t7 phage t7 phage display t7 phage nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Xie B., Tassi E., Swift MR., McDonnell K., Bowden ET., Wang S., Ueda Y., Tomita Y., Riegel AT., Wellstein A. BioCreative - Critical Assessment of Information Extraction systems in Biology Biopanning experiment using N-terminal deletion mutants of FGFBP1 wellstea@georgetown.edu Accepted 2006-AUG-4 by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation fgfp1_human Fibroblast growth factor-binding protein 1 17 kDa heparin-binding growth factor-binding protein FGFBP1 FGFBP HBP17 protein protein human Homo sapiens Human MKICSLTLLSFLLLAAQVLLVEGKKKVKNGLHSKVVSEQKDTLGNTQIKQKSRPGNKGKFVTKDQANCRWAATEQEEGISLKVECTQLDHEFSCVFAGNPTSCLKLKDERVYWKQVARNLRSQKDICRYSKTAVKTRVCRKDFPESSLKLVSSTLFGNTKPRKEKTEMSPREHIKGKETTPSSLAVTQTMATKAPECVEDPDMANQRKTALEFCGETWSSLCTFFLSIVQDTSC AAF4209F29F2D058 fgf2_human Fibroblast growth factor 2 Basic fibroblast growth factor FGF2 FGFB Heparin-binding growth factor 2 protein protein human Homo sapiens Human MVGVGGGDVEDVTPRPGGCQISGRGARGCNGIPGAAAWEAALPRRRPRRHPSVNPRSRAAGSPRTRGRRTEERPSGSRLGDRGRGRALPGGRLGGRGRGRAPERVGGRGRGRGTAAPRAAPAARGSRPGPAGTMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS DIP protein P09038 original sequence version: 169 DIP protein P09038 original sequence version: 129 EAE98552A39D5E19 fgfbp1-fgf2-1 3 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction 1 & 2 Binding of FGF-BP1-expressing phage to immobilized FGF-2. fgfbp1-fgf2-2 1 5 unspecified role unspecified role bait bait i125 region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey direct interaction direct interaction 3A competition of Malt-BP1 for binding of radiolabeled FGF-2 to immobilized full-length recombinant FGF-BP1 A fusion protein of MALT and the C-terminal fragment of FGF-BP1 competed for binding. fgfbp1-fgf2-3 2 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction 4A & 4B Surface plasmon resonance analysis of the 42-amino-acid C-terminal FGF-BP1 fragment (BP1 C-term) binding to immobilized FGF-2