IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Regulation of transactivation function of the aryl hydrocarbon receptor by the Epstein-Barr virus-encoded EBNA-3 protein. Regulation of transactivation function of the aryl hydrocarbon receptor by the Epstein-Barr virus-encoded EBNA-3 protein. J. Biol. Chem. (0021-9258) 2006 imex curation Kashuba EV., Gradin K., Isaguliants M., Szekely L., Poellinger L., Klein G., Kazlauskas A. BioCreative - Critical Assessment of Information Extraction systems in Biology Arnt origin of paper determined after going three references into the paper. lorenz.poellinger@cmb.ki.se 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Kashuba EV., Gradin K., Isaguliants M., Szekely L., Poellinger L., Klein G., Kazlauskas A. BioCreative - Critical Assessment of Information Extraction systems in Biology Arnt origin of paper determined after going three references into the paper. lorenz.poellinger@cmb.ki.se Accepted 2006-SEP-15 by DAVET. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Regulation of transactivation function of the aryl hydrocarbon receptor by the Epstein-Barr virus-encoded EBNA-3 protein. Regulation of transactivation function of the aryl hydrocarbon receptor by the Epstein-Barr virus-encoded EBNA-3 protein. J. Biol. Chem. (0021-9258) 2006 imex curation Kashuba EV., Gradin K., Isaguliants M., Szekely L., Poellinger L., Klein G., Kazlauskas A. BioCreative - Critical Assessment of Information Extraction systems in Biology Arnt origin of paper determined after going three references into the paper. lorenz.poellinger@cmb.ki.se 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 pull down pull down western blot western blot Immuno blot Immuno blot Kashuba EV., Gradin K., Isaguliants M., Szekely L., Poellinger L., Klein G., Kazlauskas A. BioCreative - Critical Assessment of Information Extraction systems in Biology Arnt origin of paper determined after going three references into the paper. lorenz.poellinger@cmb.ki.se Accepted 2006-SEP-15 by DAVET. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Regulation of transactivation function of the aryl hydrocarbon receptor by the Epstein-Barr virus-encoded EBNA-3 protein. Regulation of transactivation function of the aryl hydrocarbon receptor by the Epstein-Barr virus-encoded EBNA-3 protein. J. Biol. Chem. (0021-9258) 2006 imex curation Kashuba EV., Gradin K., Isaguliants M., Szekely L., Poellinger L., Klein G., Kazlauskas A. BioCreative - Critical Assessment of Information Extraction systems in Biology Arnt origin of paper determined after going three references into the paper. lorenz.poellinger@cmb.ki.se 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kashuba EV., Gradin K., Isaguliants M., Szekely L., Poellinger L., Klein G., Kazlauskas A. BioCreative - Critical Assessment of Information Extraction systems in Biology Arnt origin of paper determined after going three references into the paper. lorenz.poellinger@cmb.ki.se Accepted 2006-SEP-15 by DAVET. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Regulation of transactivation function of the aryl hydrocarbon receptor by the Epstein-Barr virus-encoded EBNA-3 protein. Regulation of transactivation function of the aryl hydrocarbon receptor by the Epstein-Barr virus-encoded EBNA-3 protein. J. Biol. Chem. (0021-9258) 2006 imex curation Kashuba EV., Gradin K., Isaguliants M., Szekely L., Poellinger L., Klein G., Kazlauskas A. BioCreative - Critical Assessment of Information Extraction systems in Biology Arnt origin of paper determined after going three references into the paper. lorenz.poellinger@cmb.ki.se 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kashuba EV., Gradin K., Isaguliants M., Szekely L., Poellinger L., Klein G., Kazlauskas A. BioCreative - Critical Assessment of Information Extraction systems in Biology Arnt origin of paper determined after going three references into the paper. lorenz.poellinger@cmb.ki.se Accepted 2006-SEP-15 by DAVET. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Regulation of transactivation function of the aryl hydrocarbon receptor by the Epstein-Barr virus-encoded EBNA-3 protein. Regulation of transactivation function of the aryl hydrocarbon receptor by the Epstein-Barr virus-encoded EBNA-3 protein. J. Biol. Chem. (0021-9258) 2006 imex curation Kashuba EV., Gradin K., Isaguliants M., Szekely L., Poellinger L., Klein G., Kazlauskas A. BioCreative - Critical Assessment of Information Extraction systems in Biology Arnt origin of paper determined after going three references into the paper. lorenz.poellinger@cmb.ki.se 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Kashuba EV., Gradin K., Isaguliants M., Szekely L., Poellinger L., Klein G., Kazlauskas A. BioCreative - Critical Assessment of Information Extraction systems in Biology Arnt origin of paper determined after going three references into the paper. AhR-GFP, EBNA3, GFP-XAP2, and FLAG-XAP2 were used. lorenz.poellinger@cmb.ki.se Accepted 2006-SEP-15 by DAVET. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation ebna3_ebvb9 Epstein-Barr nuclear antigen 3 Epstein-Barr nuclear antigen 3A BLRF3-BERF1 EBNA3 protein protein ebvb9 Epstein-Barr virus (strain B95-8) (HHV-4) Human herpesvirus 4 HHV-4 MDKDRPGPPALDDNMEEEVPSTSVVQEQVSAGDWENVLIELSDSSSEKEAEDAHLEPAQKGTKRKRVDHDAGGSAPARPMLPPQPDLPGREAILRRFPLDLRTLLQAIGAAATRIDTRAIDQFFGSQISNTEMYIMYAMAIRQAIRDRRRNPASRRDQAKWRLQTLAAGWPMGYQAYSSWMYSYTDHQTTPTFVHLQATLGCTGGRRCHVTFSAGTFKLPRCTPGDRQWLYVQSSVGNIVQSCNPRYSIFFDYMAIHRSLTKIWEEVLTPDQRVSFMEFLGFLQRTDLSYIKSFVSDALGTTSIQTPWIDDNPSTETAQAWNAGFLRGRAYGIDLLRTEGEHVEGATGETREESEDTESDGDDEDLPCIVSRGGPKVKRPPIFIRRLHRLLLMRAGKRTEQGKEVLEKARGSTYGTPRPPVPKPRPEVPQSDETATSHGSAQVPEPPTIHLAAQGMAYPLHEQHGMAPCPVAQAPPTPLPPVSPGDQLPGVFSDGRVACAPVPAPAGPIVRPWEPSLTQAAGQAFAPVRPQHMPVEPVPVPTVALERPVYPKPVRPAPPKIAMQGPGETSGIRRARERWRPAPWTPNPPRSPSQMSVRDRLARLRAEAQVKQASVEVQPPQLTQVSPQQPMEGPLVPEQQMFPGAPFSQVADVVRAPGVPAMQPQYFDLPLIQPISQGAPVAPLRASMGPVPPVPATQPQYFDIPLTEPINQGASAAHFLPQQPMEGPLVPEQWMFPGAALSQSVRPGVAQSQYFDLPLTQPINHGAPAAHFLHQPPMEGPWVPEQWMFQGAPPSQGTDVVQHQLDALGYTLHGLNHPGVPVSPAVNQYHLSQAAFGLPIDEDESGEGSDTSEPCEALDLSIHGRPCPQAPEWPVQEEGGQDATEVLDLSIHGRPRPRTPEWPVQGEGGQNVTGPETRRVVVSAVVHMCQDDEFPDLQDPPDEA 3413D9EA6BA7F06F ahr_human Aryl hydrocarbon receptor AHR BHLHE76 Class E basic helix-loop-helix protein 76 protein protein human Homo sapiens Human MNSSSANITYASRKRRKPVQKTVKPIPAEGIKSNPSKRHRDRLNTELDRLASLLPFPQDVINKLDKLSVLRLSVSYLRAKSFFDVALKSSPTERNGGQDNCRAANFREGLNLQEGEFLLQALNGFVLVVTTDALVFYASSTIQDYLGFQQSDVIHQSVYELIHTEDRAEFQRQLHWALNPSQCTESGQGIEEATGLPQTVVCYNPDQIPPENSPLMERCFICRLRCLLDNSSGFLAMNFQGKLKYLHGQKKKGKDGSILPPQLALFAIATPLQPPSILEIRTKNFIFRTKHKLDFTPIGCDAKGRIVLGYTEAELCTRGSGYQFIHAADMLYCAESHIRMIKTGESGMIVFRLLTKNNRWTWVQSNARLLYKNGRPDYIIVTQRPLTDEEGTEHLRKRNTKLPFMFTTGEAVLYEATNPFPAIMDPLPLRTKNGTSGKDSATTSTLSKDSLNPSSLLAAMMQQDESIYLYPASSTSSTAPFENNFFNESMNECRNWQDNTAPMGNDTILKHEQIDQPQDVNSFAGGHPGLFQDSKNSDLYSIMKNLGIDFEDIRHMQNEKFFRNDFSGEVDFRDIDLTDEILTYVQDSLSKSPFIPSDYQQQQSLALNSSCMVQEHLHLEQQQQHHQKQVVVEPQQQLCQKMKHMQVNGMFENWNSNQFVPFNCPQQDPQQYNVFTDLHGISQEFPYKSEMDSMPYTQNFISCNQPVLPQHSKCTELDYPMGSFEPSPYPTTSSLEDFVTCLQLPENQKHGLNPQSAIITPQTCYAGAVSMYQCQPEPQHTHVGQMQYNPVLPGQQAFLNKFQNGVLNETYPAELNNINNTQTTTHLQPLHHPSEARPFPDLTSSGFL DIP protein P35869 original sequence version: 109 1BFE022871B7B028 aip_human AH receptor-interacting protein Aryl-hydrocarbon receptor-interacting protein Immunophilin homolog ARA9 HBV X-associated protein 2 AIP XAP2 protein protein human Homo sapiens Human MADIIARLREDGIQKRVIQEGRGELPDFQDGTKATFHYRTLHSDDEGTVLDDSRARGKPMELIIGKKFKLPVWETIVCTMREGEIAQFLCDIKHVVLYPLVAKSLRNIAVGKDPLEGQRHCCGVAQMREHSSLGHADLDALQQNPQPLIFHMEMLKVESPGTYQQDPWAMTDEEKAKAVPLIHQEGNRLYREGHVKEAAAKYYDAIACLKNLQMKEQPGSPEWIQLDQQITPLLLNYCQCKLVVEEYYEVLDHCSSILNKYDDNVKAYFKRGKAHAAVWNAQEAQADFAKVLELDPALAPVVSRELQALEARIRQKDEEDKARFRGIFSH DIP protein O00170 original sequence version: 99 9302AA6D0F8D8F51 ebna3-ahr 1 6 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction In this study, we show that EBNA-3 interacts specifically with AhR. 1B ebna3-ahr-1 2 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association In this study, we show that EBNA-3 interacts specifically with AhR 1C, 1D Interaction carried out in the presence of XAP2. Stability is affected by The presence of XAP1. ahr-aip 5 7 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component colocalization colocalization XAP2 retains the nonactivated AhR in the cell cytoplasm 3 In the presence of TCDD some localisation to nucleus was found. ahr-aip-1 5 8 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization XAP2 retains the nonactivated AhR in the cell cytoplasm 4 In the presence of TCDD some localisation to nucleus was found. ahr-aip-2 3 7 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association EBNA-3 interacts with an immunophilin-like protein XAP2/ARA9/AIP 5 The increase in EBNA3 expression decreased amount of AhR Coprecipitated with XAP2. ahr-ebna3 3 7 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association In this study, we show that EBNA-3 interacts specifically with AhR 5C, 5D The increase in XAP2 expression decreased amount of AhR Coprecipitated with EBNA3. ahr-ebna3-1 4 6 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey physical association physical association EBNA-3 interacts with an immunophilin-like protein XAP2/ARA9/AIP 6 Interaction was increased in the presence of TCDD and ARNT together. Interaction is decreased in the presence of ARNT or TCDD or XAP2 alone. The decrease was maximum in the presence of both XAP2 and ARNT.