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    <entry>
        <source releaseDate="2020-07-09+01:00">
            <names>
                <shortLabel>IntAct</shortLabel>
                <fullName>European Bioinformatics Institute</fullName>
                <alias typeAc="MI:1041" type="synonym">IntAct</alias>
                <alias typeAc="MI:1041" type="synonym">intact</alias>
            </names>
            <bibref>
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                </xref>
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                <secondaryRef db="pubmed" dbAc="MI:0446" id="22121220" refType="method reference" refTypeAc="MI:0357"/>
            </xref>
            <attributeList>
                <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
                <attribute name="search-url" nameAc="MI:0615">http://www.ebi.ac.uk/intact/query/${ac}</attribute>
                <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
                <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
                <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact</attribute>
                <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
                <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
                <attribute name="url">http://www.ebi.ac.uk/</attribute>
            </attributeList>
        </source>
        <experimentList>
            <experimentDescription id="3718066">
                <names>
                    <shortLabel>kim-2010-42</shortLabel>
                </names>
                <bibref>
                    <xref>
                        <primaryRef db="pubmed" dbAc="MI:0446" id="20512978" refType="primary-reference" refTypeAc="MI:0358"/>
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                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-25642710" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="imex" dbAc="MI:0670" id="IM-28096" refType="imex-primary" refTypeAc="MI:0662"/>
                </xref>
                <hostOrganismList>
                    <hostOrganism ncbiTaxId="-1">
                        <names>
                            <shortLabel>in vitro</shortLabel>
                            <fullName>In vitro</fullName>
                        </names>
                    </hostOrganism>
                </hostOrganismList>
                <interactionDetectionMethod>
                    <names>
                        <shortLabel>x-ray diffraction</shortLabel>
                        <fullName>x-ray crystallography</fullName>
                        <alias typeAc="MI:0303" type="go synonym">X-ray</alias>
                        <alias typeAc="MI:1041" type="synonym">X-ray</alias>
                        <alias typeAc="MI:1041" type="synonym">x-ray diffraction</alias>
                    </names>
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                    </xref>
                </interactionDetectionMethod>
                <participantIdentificationMethod>
                    <names>
                        <shortLabel>predetermined</shortLabel>
                        <fullName>predetermined participant</fullName>
                        <alias typeAc="MI:1041" type="synonym">predetermined</alias>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </participantIdentificationMethod>
                <attributeList>
                    <attribute name="author-list" nameAc="MI:0636">Kim DY., Kwon E., Choi J., Hwang HY., Kim KK.</attribute>
                    <attribute name="contact-email" nameAc="MI:0634">kkim@med.skku.sc.kr</attribute>
                    <attribute name="journal" nameAc="MI:0885">Protein science : a publication of the Protein Society</attribute>
                    <attribute name="publication year" nameAc="MI:0886">2010</attribute>
                    <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
                    <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
                    <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
                </attributeList>
            </experimentDescription>
        </experimentList>
        <interactorList>
            <interactor id="3718067">
                <names>
                    <shortLabel>rsea_ecoli</shortLabel>
                    <fullName>Anti-sigma-E factor RseA</fullName>
                    <alias typeAc="MI:0302" type="gene name synonym">yfiJ</alias>
                    <alias typeAc="MI:0301" type="gene name">rseA</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">mclA</alias>
                    <alias typeAc="MI:0305" type="locus name">b2572</alias>
                    <alias typeAc="MI:0305" type="locus name">JW2556</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">Regulator of SigE</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">Sigma-E anti-sigma factor RseA</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">Sigma-E factor negative regulatory protein</alias>
                </names>
                <xref>
                    <primaryRef db="uniprotkb" dbAc="MI:0486" id="P0AFX7" version="SP_21" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q2MAF8" version="SP_21" refType="secondary-ac" refTypeAc="MI:0360"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="WP_001168459.1"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="NP_417067.1"/>
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                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR026279" version="SP_116"/>
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                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0016989" secondary="sigma factor antagonist activity" version="SP_116"/>
                    <secondaryRef db="dip" dbAc="MI:0465" id="DIP-39581N"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
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                    </xref>
                </interactorType>
                <organism ncbiTaxId="83333">
                    <names>
                        <shortLabel>ecoli</shortLabel>
                        <fullName>Escherichia coli (strain K12)</fullName>
                    </names>
                </organism>
                <sequence>MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMRGDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLTQMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANNGQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ</sequence>
            </interactor>
            <interactor id="3718068">
                <names>
                    <shortLabel>rseb_ecoli</shortLabel>
                    <fullName>Sigma-E factor regulatory protein RseB</fullName>
                    <alias typeAc="MI:0301" type="gene name">rseB</alias>
                    <alias typeAc="MI:0305" type="locus name">b2571</alias>
                    <alias typeAc="MI:0305" type="locus name">JW2555</alias>
                </names>
                <xref>
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                    <secondaryRef db="refseq" dbAc="MI:0481" id="NP_417066.1"/>
                    <secondaryRef db="ensemblbacteria" id="AAC75624" version="SP_110" refType="transcript"/>
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                    <secondaryRef db="dip" dbAc="MI:0465" id="DIP-10802N"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0045152" secondary="antisigma factor binding" version="SP_110"/>
                    <secondaryRef db="ensemblbacteria" id="BAE76747" version="SP_110" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0008289" secondary="lipid binding" version="SP_110"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3M4W" version="SP_110"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2P4B" version="SP_110"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2V42" version="SP_110"/>
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                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2V43" version="SP_110"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="WP_000812053.1"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR033434" version="SP_110"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0032885" secondary="regulation of polysaccharide biosynthetic process" version="SP_110"/>
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                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR033436" version="SP_110"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1135231" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q2MAF9" version="SP_15" refType="secondary-ac" refTypeAc="MI:0360"/>
                    <secondaryRef db="ensemblbacteria" id="b2571" version="SP_110" refType="gene"/>
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                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0030288" secondary="outer membrane-bounded periplasmic space" version="SP_110"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
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                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactorType>
                <organism ncbiTaxId="83333">
                    <names>
                        <shortLabel>ecoli</shortLabel>
                        <fullName>Escherichia coli (strain K12)</fullName>
                    </names>
                </organism>
                <sequence>MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYELSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTLNGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWMDTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKAKFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQMLRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ</sequence>
            </interactor>
        </interactorList>
        <interactionList>
            <interaction imexId="IM-28096-1" id="3718069">
                <names>
                    <shortLabel>rsea-rseb-8</shortLabel>
                </names>
                <xref>
                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-25642717" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="imex" dbAc="MI:0670" id="IM-28096-1" refType="imex-primary" refTypeAc="MI:0662"/>
                    <secondaryRef db="pdbe" dbAc="MI:0472" id="3m4w"/>
                </xref>
                <experimentList>
                    <experimentRef>3718066</experimentRef>
                </experimentList>
                <participantList>
                    <participant id="3718070">
                        <names>
                            <shortLabel>N/A</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-25642720" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>3718067</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>neutral component</shortLabel>
                                    <fullName>neutral component</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
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                            </experimentalRole>
                        </experimentalRoleList>
                        <experimentalPreparationList>
                            <experimentalPreparation>
                                <names>
                                    <shortLabel>purified</shortLabel>
                                    <fullName>purified</fullName>
                                </names>
                                <xref>
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                                </xref>
                            </experimentalPreparation>
                        </experimentalPreparationList>
                        <featureList>
                            <feature id="3718071">
                                <names>
                                    <shortLabel>Anti-sigma-E factor RseA C-terminal domain</shortLabel>
                                </names>
                                <xref>
                                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-25642723" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                                <featureType>
                                    <names>
                                        <shortLabel>sufficient to bind</shortLabel>
                                        <fullName>sufficient binding region</fullName>
                                        <alias typeAc="MI:1041" type="synonym">sufficient to bind</alias>
                                    </names>
                                    <xref>
                                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0442" refType="identity" refTypeAc="MI:0356"/>
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                                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608899" refType="identity" refTypeAc="MI:0356"/>
                                    </xref>
                                </featureType>
                                <featureRangeList>
                                    <featureRange>
                                        <startStatus>
                                            <names>
                                                <shortLabel>certain</shortLabel>
                                                <fullName>certain sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">certain</alias>
                                            </names>
                                            <xref>
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                                            </xref>
                                        </startStatus>
                                        <begin position="121"/>
                                        <endStatus>
                                            <names>
                                                <shortLabel>certain</shortLabel>
                                                <fullName>certain sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">certain</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                            </xref>
                                        </endStatus>
                                        <end position="216"/>
                                        <isLink>false</isLink>
                                    </featureRange>
                                </featureRangeList>
                            </feature>
                        </featureList>
                        <hostOrganismList>
                            <hostOrganism ncbiTaxId="469008">
                                <names>
                                    <shortLabel>ecobd</shortLabel>
                                    <fullName>Escherichia coli (strain B / BL21-DE3)</fullName>
                                </names>
                            </hostOrganism>
                        </hostOrganismList>
                        <attributeList>
                            <attribute name="comment" nameAc="MI:0612">Stoichiometry: 1.0</attribute>
                        </attributeList>
                    </participant>
                    <participant id="3718072">
                        <names>
                            <shortLabel>N/A</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-25642719" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>3718068</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>neutral component</shortLabel>
                                    <fullName>neutral component</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                        <experimentalPreparationList>
                            <experimentalPreparation>
                                <names>
                                    <shortLabel>purified</shortLabel>
                                    <fullName>purified</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                </xref>
                            </experimentalPreparation>
                        </experimentalPreparationList>
                        <featureList>
                            <feature id="3718073">
                                <names>
                                    <shortLabel>Sigma-E factor regulatory protein RseB</shortLabel>
                                </names>
                                <xref>
                                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-25642721" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                                <featureType>
                                    <names>
                                        <shortLabel>sufficient to bind</shortLabel>
                                        <fullName>sufficient binding region</fullName>
                                        <alias typeAc="MI:1041" type="synonym">sufficient to bind</alias>
                                    </names>
                                    <xref>
                                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0442" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608899" refType="identity" refTypeAc="MI:0356"/>
                                    </xref>
                                </featureType>
                                <featureRangeList>
                                    <featureRange>
                                        <startStatus>
                                            <names>
                                                <shortLabel>certain</shortLabel>
                                                <fullName>certain sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">certain</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                            </xref>
                                        </startStatus>
                                        <begin position="24"/>
                                        <endStatus>
                                            <names>
                                                <shortLabel>certain</shortLabel>
                                                <fullName>certain sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">certain</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                            </xref>
                                        </endStatus>
                                        <end position="318"/>
                                        <isLink>false</isLink>
                                    </featureRange>
                                </featureRangeList>
                            </feature>
                        </featureList>
                        <hostOrganismList>
                            <hostOrganism ncbiTaxId="469008">
                                <names>
                                    <shortLabel>ecobd</shortLabel>
                                    <fullName>Escherichia coli (strain B / BL21-DE3)</fullName>
                                </names>
                            </hostOrganism>
                        </hostOrganismList>
                        <attributeList>
                            <attribute name="comment" nameAc="MI:0612">Stoichiometry: 1.0</attribute>
                        </attributeList>
                    </participant>
                </participantList>
                <inferredInteractionList>
                    <inferredInteraction>
                        <participant>
                            <participantFeatureRef>3718073</participantFeatureRef>
                        </participant>
                        <participant>
                            <participantFeatureRef>3718071</participantFeatureRef>
                        </participant>
                    </inferredInteraction>
                </inferredInteractionList>
                <interactionType>
                    <names>
                        <shortLabel>direct interaction</shortLabel>
                        <fullName>direct interaction</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </interactionType>
                <modelled>false</modelled>
                <intraMolecular>false</intraMolecular>
                <negative>false</negative>
                <attributeList>
                    <attribute name="figure legend" nameAc="MI:0599">Fig. 1</attribute>
                    <attribute name="3d-resolution" nameAc="MI:0632">2.3</attribute>
                    <attribute name="3d-r-factors" nameAc="MI:0631">23.9/27.2</attribute>
                </attributeList>
            </interaction>
        </interactionList>
    </entry>
</entrySet>
