DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yoo-2018-1 ecoli Escherichia coli (strain K12) 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Yoo J., Wu M., Yin Y., Herzik MA., Lander GC., Lee SY. seok-yong.lee@duke.edu,glander@scripps.edu Science (New York, N.Y.) 2018 imex curation Only protein-protein interactions imex curation yoo-2018-2 ecoli Escherichia coli (strain K12) crosslink cross-linking study crosslink predetermined predetermined participant predetermined Yoo J., Wu M., Yin Y., Herzik MA., Lander GC., Lee SY. Crosslinker - BS3 (bis(sulfosuccinimidyl)suberate) seok-yong.lee@duke.edu,glander@scripps.edu Science (New York, N.Y.) 2018 imex curation Only protein-protein interactions imex curation yoo-2018-3 ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yoo J., Wu M., Yin Y., Herzik MA., Lander GC., Lee SY. seok-yong.lee@duke.edu,glander@scripps.edu Science (New York, N.Y.) 2018 imex curation Only protein-protein interactions imex curation q7s4i4_neucr Calcium uniporter protein NCU08166 protein protein neucr Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) MNCVRMRLQCRLMPSSNTLARWCLESPQKNLTLHRPRISNVALRQASTSVSPKTRETEAEAKAKKLDQKRLDEHEEEVRAREQQVRRPWHREGADKPPVEGNADPIAKGKLLTTPTRLLKLILPLPLRVEKDQKNNGRNNEYGRSISLNSDIQPLALLIHPQQPLSYVERLIQAELPPVVENGQEKIPNVYFRAEDSEQGDQKPTSRAEARSKDDGGEPSEYNTNLSHVASYSGLGHRGPKRSSQDKRWVRWSSSTEMGDFIRDAARGREFAIEIEGYNIEMRVSVPSFGDRTYYMRQRLRKMSSEIDGLAKIKHECDLLAHRSAHRLAKGGFGLLAGWWGVVYYVTFHTEFGWDLVEPVTYLAGLTTIMGGYLWFLYINKDLSYKAAMNVTVSRRQHALYEMKGFDIERWEQLVQDANALRREIRVIAVEYDVDWDETRDVGEDVKDVLDEERSRRDDEHRSIEKEKDEKFTEDEKRKRKKDKESKETSGDS hjj-1 3468634 N/A 3468637 unspecified role unspecified role neutral component neutral component purified purified Binding Region Binding Region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q7S4I4:p.Tyr232Ala Tyr232Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 4.0 direct interaction direct interaction false false false Figure 1, Table S1 q7s4i4_neucr-1 3468635 N/A 3468637 unspecified role unspecified role neutral component neutral component purified purified Binding Region Binding Region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q7S4I4:p.Tyr232Ala Tyr232Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 4.0 direct interaction direct interaction false false false Fig. S2 (A) q7s4i4_neucr-2 3468636 N/A 3468637 unspecified role unspecified role neutral component neutral component purified purified Binding Region Binding Region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q7S4I4:p.Tyr232Ala Tyr232Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 4.0 direct interaction direct interaction false false false Fig.S2.(B)