MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint coutts-2007-1 Mdm2 targets the p53 transcription cofactor JMY for degradation. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip antibody detection identification by antibody antibody detection Coutts AS., Boulahbel H., Graham A., La Thangue NB. Cancer - Interactions investigated in the context of cancer U2OS cells have been transfected with JMY and MDM2 expression vectors. nick.lathangue@ndcls.ox.ac.uk EMBO Rep. (1469-221X) 2007 Only protein-protein interactions imex curation coutts-2007-2 Mdm2 targets the p53 transcription cofactor JMY for degradation. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip antibody detection identification by antibody antibody detection Coutts AS., Boulahbel H., Graham A., La Thangue NB. Cancer - Interactions investigated in the context of cancer U2OS cells have been transfected with JMY and MDM2 expression vectors. nick.lathangue@ndcls.ox.ac.uk EMBO Rep. (1469-221X) 2007 Only protein-protein interactions imex curation mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP jmy_human Junction-mediating and -regulatory protein JMY protein protein human Homo sapiens Human MSFALEETLESDWVAVRPHVFDEREKHKFVFIVAWNEIEGKFAITCHNRTAQRQRSGSREQAGARGGAEAGGAASDGSRGPGSPAGRGRPEATASATLVRSPGPRRSSAWAEGGSPRSTRSLLGDPRLRSPGSKGAESRLRSPVRAKPIPGQKTSEADDAAGAAAAAARPAPREAQVSSVRIVSASGTVSEEIEVLEMVKEDEAPLALSDAEQPPPATELESPAEECSWAGLFSFQDLRAVHQQLCSVNSQLEPCLPVFPEEPSGMWTVLFGGAPEMTEQEIDTLCYQLQVYLGHGLDTCGWKILSQVLFTETDDPEEYYESLSELRQKGYEEVLQRARKRIQELLDKHKNTESMVELLDLYQMEDEAYSSLAEATTELYQYLLQPFRDMRELAMLRRQQIKISMENDYLGPRRIESLQKEDADWQRKAHMAVLSIQDLTVKYFEITAKAQKAVYDRMRADQKKFGKASWAAAAERMEKLQYAVSKETLQMMRAKEICLEQRKHALKEEMQSLRGGTEAIARLDQLEADYYDLQLQLYEVQFEILKCEELLLTAQLESIKRLISEKRDEVVYYDTYESMEAMLEKEEMAASAYLQREELQKLQQKARQLEARRGRVSAKKSYLRNKKEICIAKHNEKIQQRTRIEDEYRTHHTVQLKREKLHDEEERKSAWVSQERQRTLDRLRTFKQRYPGQVILKSTRLRLAHARRKGAASPVLQEDHCDSLPSVLQVEEKTEEVGEGRVKRGPSQTTEPQSLVQLEDTSLTQLEATSLPLSGVTSELPPTISLPLLNNNLEPCSVTINPLPSPLPPTPPPPPPPPPPPPPPPLPVAKDSGPETLEKDLPRKEGNEKRIPKSASAPSAHLFDSSQLVSARKKLRKTAEGLQRRRVSSPMDEVLASLKRGSFHLKKVEQRTLPPFPDEDDSNNILAQIRKGVKLKKVQKDVLRESFTLLPDTDPLTRSIHEALRRIKEASPESEDEEEALPCTDWEN jmy_mouse Junction-mediating and -regulatory protein Jmy protein protein mouse Mus musculus Mouse MSFALEETLESDWVAVRPHVFDEREKHKFVFIVAWNEIEGKFAITCHNRTAQRQRSGSREQAGTPASDGSRGPGSPAARGRSEAAASATAALRSPGPRKSQAWAEGGSPRSARSLKGDPPRGPAGRGPESPLRSPARAKASPLRRSAESRDAIASATPVPPAPPVPPVSSVRVVSASGAVSEEIEVLEMVREDEAPQPLPDSEQPPSAAELESSAEECSWAGLFSFQDLRAVHQQLCSVNSQLEPCLPVFPEEPSGMWTVLFGGAPEMTEQEIDALCYQLQVYLGHGLDTCGWKILSQVLFTETDDPEEYYESLSELRQKGYEEVLQRARRRIQELLDKHKTIESMVELLDLYQMEDEAYSSLAEATTELYQYLLQPFRDMRELAMLRRQQIKISMENDYLGPRRIESLQKEDADWQRKAHMAVLSIQDLTVKYFEITAKAQKAVYDRMRADQKKFGKASWAAAAERMEKLQYAVSKETLQMMRAKEICLEQKKHALKEEMQSLQGGTEAIARLDQLESDYYDLQLQLYEVQFEILKCEELLLTAQLESIKRLISEKRDEVVYYDTYESMEAMLEKEEMAASVHAQREELQKLQQKARQLEARRGRVSAKKAYLRNKKEICIAKHHEKFQQRFQSEDEYRAHHTIQIKRDKLHDEEERKSAWVSQERQRTLDRLRTFKQRYPGQVILKSTRLRVAHSRRKSTASPVPCEEQCHSLPTVLQGQEKTEVGGGGSQLGPSQTAEPQSLVQLEDTSSEQLESTSLPPRAVVSSELPPPQSAPLLTSIDPKPCSVTIDPLPPPLPPTPPPPPPPPPPPPPPLPVAKDNGASTTAETLEKDALRTEGNERSIPKSASAPAAHLFDSSQLVSARKKLRKTVEGLQRRRVSSPMDEVLASLKRGSFHLKKVEQRTLPPFPDEDDSNNILAQIRKGVKLKKVQKEVLRESFTLLPDTDPLTRSIHEALRRIKEASPESEDEEEALPCTDWEN mdm2-jmy-1 576206 N/A 576208 unspecified role unspecified role bait bait N/A 576209 unspecified role unspecified role prey prey physical association physical association false false false 2e mdm2-jmy-2 576206 N/A 576209 unspecified role unspecified role bait bait N/A 576208 unspecified role unspecified role prey prey physical association physical association false false false 2e mdm2-jmy-3 576207 N/A 576208 unspecified role unspecified role bait bait human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference N/A 576210 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference physical association physical association false false false 5d MDM2 mutant cys462ala has no effect on interaction. Deleting region 58-89 of MDM2 decreases interaction strength.