IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ sorensen-2006-2 Retention of mutant low density lipoprotein receptor in endoplasmic reticulum (ER) leads to ER stress. crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sorensen S., Ranheim T., Bakken K.S., Leren T.P., Kulseth M.A. BioCreative - Critical Assessment of Information Extraction systems in Biology The interactions between chaperones and folding polypeptides are transient in nature. To prevent the release of chaperones from the substrates, cross-linking was therefore performed by adding DSP before the cells were solubilized in lysis buffer. mari.ann.kulseth@rikshospitalet.no J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation sorensen-2006-1 Retention of mutant low density lipoprotein receptor in endoplasmic reticulum (ER) leads to ER stress. crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sorensen S., Ranheim T., Bakken K.S., Leren T.P., Kulseth M.A. BioCreative - Critical Assessment of Information Extraction systems in Biology mari.ann.kulseth@rikshospitalet.no J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation g3hqm6_crigr I79_013131 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MRVLWVLGLCCVLLTFGFVRADDEVDVDGTVEEDLGKSREGSRTDDEVVQREEEAIQLDGLNASQIRELREKSEKFAFQAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALAGNEELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVIVTSKHNNDTQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVRKYSQFINFPIYVWSSKTETVEEPLEEDEAAKEEKEESDDEAAVEEEEEEKKPKTKKVEKTVWDWELMNDIKPIWQRPSKEVEEDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTSAPRGLFDEYGSKKSDYIKLYVRRVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSRETLQQHKLLKVIRKKLVRKTLDMIKKIADEKYNDTFWKEFGTNIKLGVIEDHSNRTRLAKLLRFQSSHHSTDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDESEKTKENREATEKEFEPLLNWMKDKALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRVKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPEAQVEEEPEEEPEDTTEDTEQDEEEEVDAGTEEEEEEEQETAKESTAEKDEL ldlr_human Low-density lipoprotein receptor LDLR protein protein human Homo sapiens Human MGPWGWKLRWTVALLLAAAGTAVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSCGGRVNRCIPQFWRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPDCKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDCKDMSDEVGCVNVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDNNGGCSHVCNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNLEGGYKCQCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTLDRSEYTSLIPNLRNVVALDTEVASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISRDIQAPDGLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRAIVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLLSGRLYWVDSKLHSISSIDVNGGNRKTILEDEKRLAHPFSLAVFEDKVFWTDIINEAIFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTTLSNGGCQYLCLPAPQINPHSPKFTCACPDGMLLARDMRSCLTEAEAAVATQETSTVRLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSHQALGDVAGRGNEKKPSSVRALSIVLPIVLLVFLCLGVFLLWKNWRLKNINSINFDNPVYQKTTEDEVHICHNQDGYSYPSRQMVSLEDDVA g3idt6_crigr Protein disulfide-isomerase A4 Endoplasmic reticulum resident protein 72 I79_021872 CgPICR_019838 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MKLRKAWLLVLLLALTQLLAAASATDAHEDASDTENAIEDEEEEEEDEEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIASTLKDNDPPIAVAKIDATSASMLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEIVAKVREVSQPDWTPPPEVTLLLTKDNFDDVVNNADIILVEFYAPWCGHCKKLAPEYEKAAKELSKHSPPIPLAKVDATEQTDLAKRFDVSGYPTLKIFRKGRPFDYNGPREKYGIVSYMIEQSGPPSKEILTLKQVQEFLKDGDDVVIIGVFQGDSDPGYLQYQDAANNLREDYRFHHTFNTEIAKFLKVSMGKLVLMQPEKFQSKYEPRSNVMDVQGSTEPSAIKDYVVKHALPLVGHRKTSNDAKRYTKRPLVVVYYSVDFSFDYRTATQFWRNKVLEVAKDFPEYTFAIADEEDYATEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDADTLREFVTAFKKGKLKPVIKSQPIPKNNKGPVKVVVGKTFDAIVMDPKKDVLIEFYAPWCGHCKQLEPIYTNLGKKYKGQKDLVIAKMDATANDITNDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIDEHATKKSRTKEEL q8k3h8_crigr protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MEGKWLLCLLLVLGTVAVQAHDGHDDDMIDIEDDLDDVIEEVEDSKSKSDSSTPPSPKVTYKAPVPTGEVYFADSFDRGSLSGWILSKAKKDDTDDEIAKYDGKWEVDEMKDTKLPGDKGLVLMSRAKHHAISAKLNKPFLFDTKPLIVQYEVNFQNGIECGGAYVKLLSKTSELNLDQFHDKTPYTIMFGPDKCGEDYKLHFIFRHKNPKTGVYEEKHAKRPDTDLKIYFTDKKTHLYTLILNPDNSFEILVDQYVVNSGNLLNDMTPAVNPSREIEDPEDQKXEDWDERPKIPDPDAVKPDDWDEDAPGKIPDEEATKPEGWLDDEPEYIPDPDADKPEDWDEDMDGEWEAPQIANPKCESAPGCGVWQRPLIDNPNYKGKWKPPMIDNPNYQGVWKPRKIPNPDFFEDLEPFKMTPFSAIGLELWSMTSDIFFDNFIISGDRRVVDDWANDGWGLKKAADGAAEPGVVGHMLEAAEERPWLWVVYILTVALPVFLVILFCCSGKKQSNAMEYKKTDAAQPDVKEDEGKEEEKNKGDEEEEEEKLEEKQKSDAEEDGGTGSQDEEDRKPTAEEDEILNRSPRNRKPRRE bip_crigr Endoplasmic reticulum chaperone BiP HSPA5 78 kDa glucose-regulated protein Binding-immunoglobulin protein GRP78 Heat shock protein 70 family protein 5 Heat shock protein family A member 5 Immunoglobulin heavy chain-binding protein I79_019946 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MKFPMVAAALLLLCAVRAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTSEKDEL ldlr-hsp90b1 504784 N/A 504786 unspecified role unspecified role prey prey crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 504787 unspecified role unspecified role bait bait P01130:p.Gly565Val mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association false false false The LDLR is presumed to be human as the antibody used (61099) was stated to be specific for human LDLR on the provider's (Progen Biotechnik) website. 2 C The chaperones Grp94, ERp72, and calnexin were only co-immunoprecipitated with G544V mutant LDLR in cells pretreated with cross-linker. These results indicate that the ER-resident chaperones Grp78, Grp94, ERp72, and calnexin are involved in the retention of the G544V mutant LDLR in ER. The chaperone proteins Grp94, ERp72 and calnexin only interact with the G544V mutant LDLR and not the wild-type. ldlr-pdia4 504784 N/A 504787 unspecified role unspecified role bait bait P01130:p.Gly565Val mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 504788 unspecified role unspecified role prey prey crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association false false false The LDLR is presumed to be human as the antibody used (61099) was stated to be specific for human LDLR on the provider's (Progen Biotechnik) website. 2 C The chaperones Grp94, ERp72, and calnexin were only co-immunoprecipitated with G544V mutant LDLR in cells pretreated with cross-linker. These results indicate that the ER-resident chaperones Grp78, Grp94, ERp72, and calnexin are involved in the retention of the G544V mutant LDLR in ER. The chaperone proteins Grp94, ERp72 and calnexin only interact with the G544V mutant LDLR and not the wild-type. ldlr-q8k3h8 504784 N/A 504789 unspecified role unspecified role prey prey crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 504787 unspecified role unspecified role bait bait P01130:p.Gly565Val mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association false false false The LDLR is presumed to be human as the antibody used (61099) was stated to be specific for human LDLR on the provider's (Progen Biotechnik) website. 2 C The chaperones Grp94, ERp72, and calnexin were only co-immunoprecipitated with G544V mutant LDLR in cells pretreated with cross-linker. These results indicate that the ER-resident chaperones Grp78, Grp94, ERp72, and calnexin are involved in the retention of the G544V mutant LDLR in ER. The chaperone proteins Grp94, ERp72 and calnexin only interact with the G544V mutant LDLR and not the wild-type. ldlr-hspa5 504785 N/A 504790 unspecified role unspecified role prey prey crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 504787 unspecified role unspecified role bait bait P01130:p.Gly565Val mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association false false false The LDLR is presumed to be human as the antibody used (61099) was stated to be specific for human LDLR on the provider's (Progen Biotechnik) website. 2 C Grp78 co-immunoprecipitated with the G544V mutant LDLR from cells treated with or without crosslinker.