CYP11A
CYP11A1
UniProt:P05108 CYP11A1
AKR1B1
ALDR1
UniProt:P15121 AKR1B1
Cholesterol translocates to the inner mitochondrial membrane
Cholesterol is released into the inner mitochondrial membrane
Oxidation of cholesterol
Oxidation of cholesterol to 22beta-hydroxycholesterol
cholesterol + NADPH + H+ => 22beta-hydroxycholesterol + NADP+ + H2O
22beta-hydroxycholesterol + NADPH + H+ + O2 => 20alpha,22beta-hydroxycholesterol + NADP+ + H2O
Oxidation of 22beta-hydroxycholesterol to 20alpha,22beta-hydroxycholesterol
20alpha,22beta-hydroxycholesterol + NADPH + H+ + O2 => pregnenolone + isocaproaldehyde + NADP+ + H2O
20alpha,22beta-hydroxycholesterol is cleaved by CYP11A1 to yield pregnenolone and isocaproaldehyde
Translocation of isocaproaldehyde from the mitochondrial matrix to the cytosol
Reduction of isocaproaldehyde to 4-methylpentan-1-ol
isocaproaldehyde + NADPH + H+ => 4-methylpentan-1-ol + NADP+
Pregnenolone translocates from the mitochondrial matrix to the cytosol
Pregnenolone biosynthesis