CYP11A
label: CYP11A1
label: UniProt:P05108 CYP11A1

AKR1B1
label: ALDR1
label: UniProt:P15121 AKR1B1

Cholesterol translocates to the inner mitochondrial membrane
type: GO_0003674
BFO_0000050: Pregnenolone biosynthesis
directly activates: Cholesterol is released into the inner mitochondrial membrane

Cholesterol is released into the inner mitochondrial membrane
type: GO_0003674
BFO_0000050: Pregnenolone biosynthesis
directly activates: Oxidation of cholesterol
directly activates: Catalysis1098

Oxidation of cholesterol
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005759
type: Restriction
  on property: enabled by
  some values from: CYP11A
type: GO_0008386
BFO_0000050: Pregnenolone biosynthesis
directly activates: 22beta-hydroxycholesterol + NADPH + H+ + O2 => 20alpha,22beta-hydroxycholesterol + NADP+ + H2O
directly activates: Catalysis1099
label: Oxidation of cholesterol to 22beta-hydroxycholesterol
label: cholesterol + NADPH + H+ => 22beta-hydroxycholesterol + NADP+ + H2O

22beta-hydroxycholesterol + NADPH + H+ + O2 => 20alpha,22beta-hydroxycholesterol + NADP+ + H2O
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005759
type: Restriction
  on property: enabled by
  some values from: CYP11A
type: GO_0008386
BFO_0000050: Pregnenolone biosynthesis
directly activates: 20alpha,22beta-hydroxycholesterol + NADPH + H+ + O2 => pregnenolone + isocaproaldehyde + NADP+ + H2O
directly activates: Catalysis1100
label: Oxidation of 22beta-hydroxycholesterol to 20alpha,22beta-hydroxycholesterol

20alpha,22beta-hydroxycholesterol + NADPH + H+ + O2 => pregnenolone + isocaproaldehyde + NADP+ + H2O
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005759
type: Restriction
  on property: enabled by
  some values from: CYP11A
type: GO_0008386
BFO_0000050: Pregnenolone biosynthesis
directly activates: Translocation of isocaproaldehyde from the mitochondrial matrix to the cytosol
directly activates: Pregnenolone translocates from the mitochondrial matrix to the cytosol
label: 20alpha,22beta-hydroxycholesterol is cleaved by CYP11A1 to yield pregnenolone and isocaproaldehyde

Translocation of isocaproaldehyde from the mitochondrial matrix to the cytosol
type: GO_0003674
BFO_0000050: Pregnenolone biosynthesis
directly activates: Reduction of isocaproaldehyde to 4-methylpentan-1-ol
directly activates: Catalysis1101

Reduction of isocaproaldehyde to 4-methylpentan-1-ol
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: enabled by
  some values from: AKR1B1
type: GO_0004032
BFO_0000050: Pregnenolone biosynthesis
label: isocaproaldehyde + NADPH + H+ => 4-methylpentan-1-ol + NADP+

Pregnenolone translocates from the mitochondrial matrix to the cytosol
type: GO_0003674
BFO_0000050: Pregnenolone biosynthesis
directly activates: BiochemicalReaction2558
directly activates: BiochemicalReaction2566
directly activates: Catalysis1102
directly activates: Catalysis1108

Pregnenolone biosynthesis
type: GO_0008150