SC5D
label: SC5DL
label: UniProt:O75845 SC5D

ANG1
label: TM7SF2
label: UniProt:O76062 TM7SF2

LSS
label: OSC
label: UniProt:P48449 LSS

HSD17B7
label: UNQ2563/PRO6243
label: UniProt:P56937 HSD17B7

HMGCS
label: HMGCS1
label: UniProt:Q01581 HMGCS1

LBR
label: UniProt:Q14739 LBR

EBP
label: UniProt:Q15125 EBP

PMKI
label: PMVK
label: UniProt:Q15126 PMVK

DHCR24
label: KIAA0018
label: UniProt:Q15392 DHCR24

H105E3
label: NSDHL
label: UniProt:Q15738 NSDHL

DESP4
label: ERG25
label: MSMO1
label: SC4MOL
label: UniProt:Q15800 MSMO1

CYP51
label: CYP51A1
label: UniProt:Q16850 CYP51A1

D7SR
label: DHCR7
label: UniProt:Q9UBM7 DHCR7

Condensation of acetyl CoA with acetoacetyl CoA to form HMG-CoA
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: enabled by
  some values from: HMGCS
type: GO_0004421
BFO_0000050: Cholesterol biosynthesis
directly activates: Reduction of HMG-CoA produces mevalonate
directly activates: Catalysis2206

Reduction of HMG-CoA produces mevalonate
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: GO_0004420
BFO_0000050: Cholesterol biosynthesis
directly activates: Mevalonate is phosphorylated to mevalonate-5-phosphate
directly activates: Catalysis2207

Mevalonate is phosphorylated to mevalonate-5-phosphate
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: GO_0004496
BFO_0000050: Cholesterol biosynthesis
directly activates: Mevalonate-5-phosphate is further phosphorylated.
directly activates: Catalysis2208

Mevalonate-5-phosphate is further phosphorylated.
type: Restriction
  on property: enabled by
  some values from: PMKI
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: GO_0004631
BFO_0000050: Cholesterol biosynthesis
directly activates: Mevalonate-5-pyrophosphate is decarboxylated
directly activates: Catalysis2209

Mevalonate-5-pyrophosphate is decarboxylated
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: GO_0004163
BFO_0000050: Cholesterol biosynthesis
directly activates: Isopentenyl pyrophosphate rearranges to dimethylallyl pyrophosphate
directly activates: Catalysis2210

Isopentenyl pyrophosphate rearranges to dimethylallyl pyrophosphate
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: GO_0004452
BFO_0000050: Cholesterol biosynthesis
directly activates: Addition of isopentenyl pyrophosphate to DMAPP
directly activates: Catalysis2211

Addition of isopentenyl pyrophosphate to DMAPP
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: GO_0004161
BFO_0000050: Cholesterol biosynthesis
directly activates: Another isopentenyl pyrophosphate is added to geranyl pyrophosphate
directly activates: Catalysis2212

Another isopentenyl pyrophosphate is added to geranyl pyrophosphate
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: GO_0004337
BFO_0000050: Cholesterol biosynthesis
directly activates: Two FPP molecules dimerize to form presqualene diphosphate
directly activates: Catalysis2213

Two FPP molecules dimerize to form presqualene diphosphate
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: GO_0004310
BFO_0000050: Cholesterol biosynthesis
directly activates: Reduction of presqualene diphosphate to form squalene
directly activates: Catalysis2214

Reduction of presqualene diphosphate to form squalene
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: GO_0004310
BFO_0000050: Cholesterol biosynthesis
directly activates: Squalene is oxidized to its epoxide
directly activates: Catalysis2215

Squalene is oxidized to its epoxide
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: GO_0004506
BFO_0000050: Cholesterol biosynthesis
directly activates: Squalene 2,3-epoxide cyclizes, forming lanosterol
directly activates: Catalysis2216

Squalene 2,3-epoxide cyclizes, forming lanosterol
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: Restriction
  on property: enabled by
  some values from: LSS
type: GO_0000250
BFO_0000050: Cholesterol biosynthesis
directly activates: Lanosterol is oxidatively demethylated to 4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol
directly activates: Catalysis2217

Lanosterol is oxidatively demethylated to 4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: Restriction
  on property: enabled by
  some values from: CYP51
type: GO_0008398
BFO_0000050: Cholesterol biosynthesis
directly activates: 4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol + NADPH + H+ => 4,4-dimethylcholesta-8(9),24-dien-3beta-ol + NADP+ [LBR]
directly activates: 4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol + NADPH + H+ => 4,4-dimethylcholesta-8(9),24-dien-3beta-ol + NADP+ [TM7SF2]
directly activates: Catalysis2218
directly activates: Catalysis2219
label: lanosterol + 3 NADPH + 3 H+ + 3 O2 => 4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol + 3 NADP+ + 4 H2O + formate

4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol + NADPH + H+ => 4,4-dimethylcholesta-8(9),24-dien-3beta-ol + NADP+ [LBR]
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005635
type: Restriction
  on property: enabled by
  some values from: LBR
type: GO_0050613
BFO_0000050: Cholesterol biosynthesis
directly activates: 4,4-dimethylcholesta-8(9),24-dien-3beta-ol + 3 NADPH + 3 H+ + 3 O2 => 4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol + 3 NADP+ + 4 H2O
directly activates: Catalysis2220
label: 4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol is reduced to 4,4-dimethylcholesta-8(9),24-dien-3beta-ol [LBR]

4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol + NADPH + H+ => 4,4-dimethylcholesta-8(9),24-dien-3beta-ol + NADP+ [TM7SF2]
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: Restriction
  on property: enabled by
  some values from: ANG1
type: GO_0050613
BFO_0000050: Cholesterol biosynthesis
directly activates: 4,4-dimethylcholesta-8(9),24-dien-3beta-ol + 3 NADPH + 3 H+ + 3 O2 => 4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol + 3 NADP+ + 4 H2O
directly activates: Catalysis2220
label: 4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol is reduced to 4,4-dimethylcholesta-8(9),24-dien-3beta-ol [TM7SF2]

4,4-dimethylcholesta-8(9),24-dien-3beta-ol + 3 NADPH + 3 H+ + 3 O2 => 4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol + 3 NADP+ + 4 H2O
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: Restriction
  on property: enabled by
  some values from: DESP4
type: GO_0000254
BFO_0000050: Cholesterol biosynthesis
directly activates: 4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol + NAD+ => 4-methylcholesta-8(9),24-dien-3-one + CO2 + NADH + H+
directly activates: Catalysis2221
label: 4,4-dimethylcholesta-8(9),24-dien-3beta-ol is oxidized to 4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol

4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol + NAD+ => 4-methylcholesta-8(9),24-dien-3-one + CO2 + NADH + H+
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: Restriction
  on property: enabled by
  some values from: H105E3
type: GO_0000252
BFO_0000050: Cholesterol biosynthesis
directly activates: 4-methylcholesta-8(9),24-dien-3-one + NADPH + H+ => 4-methylcholesta-8(9),24-dien-3beta-ol + NADP+
directly activates: Catalysis2222
label: 4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol is decarboxylated and oxidized to form 4-methylcholesta-8(9),24-dien-3-one

4-methylcholesta-8(9),24-dien-3-one + NADPH + H+ => 4-methylcholesta-8(9),24-dien-3beta-ol + NADP+
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: Restriction
  on property: enabled by
  some values from: HSD17B7
type: GO_0000253
BFO_0000050: Cholesterol biosynthesis
directly activates: 4-methylcholesta-8(9),24-dien-3beta-ol + 3 NADPH + 3 H+ + 3 O2 => 4-carboxycholesta-8(9),24-dien-3beta-ol + 3 NADP+ + 4 H2O
directly activates: Catalysis2223
label: 4-methylcholesta-8(9),24-dien-3-one is reduced to 4-methylcholesta-8(9),24-dien-3beta-ol

4-methylcholesta-8(9),24-dien-3beta-ol + 3 NADPH + 3 H+ + 3 O2 => 4-carboxycholesta-8(9),24-dien-3beta-ol + 3 NADP+ + 4 H2O
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: Restriction
  on property: enabled by
  some values from: DESP4
type: GO_0000254
BFO_0000050: Cholesterol biosynthesis
directly activates: 4-carboxycholesta-8(9),24-dien-3beta-ol + NAD+ => cholesta-8(9),24-dien-3-one (zymosterone) + CO2 + NADH + H+
directly activates: Catalysis2224
label: 4-methylcholesta-8(9),24-dien-3beta-ol is oxidized to 4-carboxycholesta-8(9),24-dien-3beta-ol

4-carboxycholesta-8(9),24-dien-3beta-ol + NAD+ => cholesta-8(9),24-dien-3-one (zymosterone) + CO2 + NADH + H+
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: Restriction
  on property: enabled by
  some values from: H105E3
type: GO_0000252
BFO_0000050: Cholesterol biosynthesis
directly activates: Zymosterone (cholesta-8(9),24-dien-3-one) is reduced to zymosterol (cholesta-8(9),24-dien-3beta-ol)
directly activates: Catalysis2225
label: 4-carboxycholesta-8(9),24-dien-3beta-ol is decarboxylated and oxidized to form cholesta-8(9),24-dien-3-one (zymosterone)

Zymosterone (cholesta-8(9),24-dien-3-one) is reduced to zymosterol (cholesta-8(9),24-dien-3beta-ol)
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: Restriction
  on property: enabled by
  some values from: HSD17B7
type: GO_0000253
BFO_0000050: Cholesterol biosynthesis
directly activates: Zymosterol => cholesta-7,24-dien-3beta-ol
directly activates: Catalysis2226
label: zymosterone (cholesta-8(9),24-dien-3-one) + NADPH + H+ => zymosterol (cholesta-8(9),24-dien-3beta-ol) + NADP+

Zymosterol => cholesta-7,24-dien-3beta-ol
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: Restriction
  on property: enabled by
  some values from: EBP
type: GO_0004769
BFO_0000050: Cholesterol biosynthesis
directly activates: Cholesta-7,24-dien-3beta-ol is desaturated to form cholesta-5,7,24-trien-3beta-ol
directly activates: Catalysis2227
label: Zymosterol is isomerized to cholesta-7,24-dien-3beta-ol

Cholesta-7,24-dien-3beta-ol is desaturated to form cholesta-5,7,24-trien-3beta-ol
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: Restriction
  on property: enabled by
  some values from: SC5D
type: GO_0000248
BFO_0000050: Cholesterol biosynthesis
directly activates: Cholesta-5,7,24-trien-3beta-ol is reduced to desmosterol
directly activates: Catalysis2228
label: cholesta-7,24-dien-3beta-ol + NADPH + H+ + O2 => cholesta-5,7,24-trien-3beta-ol + NADP+ + 2 H2O

Cholesta-5,7,24-trien-3beta-ol is reduced to desmosterol
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: Restriction
  on property: enabled by
  some values from: D7SR
type: GO_0047598
BFO_0000050: Cholesterol biosynthesis
directly activates: Reduction of desmosterol to cholesterol
directly activates: Catalysis2229
label: cholesta-5,7,24-trien-3beta-ol + NADPH + H+ => desmosterol + NADP+

Reduction of desmosterol to cholesterol
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005789
type: Restriction
  on property: enabled by
  some values from: DHCR24
type: GO_0050614
BFO_0000050: Cholesterol biosynthesis
label: desmosterol + NADPH + H+ => cholesterol + NADP+

Cholesterol biosynthesis
type: GO_0008150