SC5D
SC5DL
UniProt:O75845 SC5D
ANG1
TM7SF2
UniProt:O76062 TM7SF2
LSS
OSC
UniProt:P48449 LSS
HSD17B7
UniProt:P56937 HSD17B7
HMGCS
HMGCS1
UniProt:Q01581 HMGCS1
LBR
UniProt:Q14739 LBR
EBP
UniProt:Q15125 EBP
PMKI
PMVK
UniProt:Q15126 PMVK
DHCR24
KIAA0018
UniProt:Q15392 DHCR24
H105E3
NSDHL
UniProt:Q15738 NSDHL
DESP4
ERG25
MSMO1
SC4MOL
UniProt:Q15800 MSMO1
CYP51
CYP51A1
UniProt:Q16850 CYP51A1
D7SR
DHCR7
UniProt:Q9UBM7 DHCR7
Condensation of acetyl CoA with acetoacetyl CoA to form HMG-CoA
Reduction of HMG-CoA produces mevalonate
Mevalonate is phosphorylated to mevalonate-5-phosphate
Mevalonate-5-phosphate is further phosphorylated.
Mevalonate-5-pyrophosphate is decarboxylated
Isopentenyl pyrophosphate rearranges to dimethylallyl pyrophosphate
Addition of isopentenyl pyrophosphate to DMAPP
Another isopentenyl pyrophosphate is added to geranyl pyrophosphate
Two FPP molecules dimerize to form presqualene diphosphate
Reduction of presqualene diphosphate to form squalene
Squalene is oxidized to its epoxide
Squalene 2,3-epoxide cyclizes, forming lanosterol
Lanosterol is oxidatively demethylated to 4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol
lanosterol + 3 NADPH + 3 H+ + 3 O2 => 4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol + 3 NADP+ + 4 H2O + formate
4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol + NADPH + H+ => 4,4-dimethylcholesta-8(9),24-dien-3beta-ol + NADP+ [LBR]
4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol is reduced to 4,4-dimethylcholesta-8(9),24-dien-3beta-ol [LBR]
4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol + NADPH + H+ => 4,4-dimethylcholesta-8(9),24-dien-3beta-ol + NADP+ [TM7SF2]
4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol is reduced to 4,4-dimethylcholesta-8(9),24-dien-3beta-ol [TM7SF2]
4,4-dimethylcholesta-8(9),24-dien-3beta-ol + 3 NADPH + 3 H+ + 3 O2 => 4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol + 3 NADP+ + 4 H2O
4,4-dimethylcholesta-8(9),24-dien-3beta-ol is oxidized to 4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol
4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol + NAD+ => 4-methylcholesta-8(9),24-dien-3-one + CO2 + NADH + H+
4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol is decarboxylated and oxidized to form 4-methylcholesta-8(9),24-dien-3-one
4-methylcholesta-8(9),24-dien-3-one + NADPH + H+ => 4-methylcholesta-8(9),24-dien-3beta-ol + NADP+
4-methylcholesta-8(9),24-dien-3-one is reduced to 4-methylcholesta-8(9),24-dien-3beta-ol
4-methylcholesta-8(9),24-dien-3beta-ol + 3 NADPH + 3 H+ + 3 O2 => 4-carboxycholesta-8(9),24-dien-3beta-ol + 3 NADP+ + 4 H2O
4-methylcholesta-8(9),24-dien-3beta-ol is oxidized to 4-carboxycholesta-8(9),24-dien-3beta-ol
4-carboxycholesta-8(9),24-dien-3beta-ol + NAD+ => cholesta-8(9),24-dien-3-one (zymosterone) + CO2 + NADH + H+
4-carboxycholesta-8(9),24-dien-3beta-ol is decarboxylated and oxidized to form cholesta-8(9),24-dien-3-one (zymosterone)
Zymosterone (cholesta-8(9),24-dien-3-one) is reduced to zymosterol (cholesta-8(9),24-dien-3beta-ol)
zymosterone (cholesta-8(9),24-dien-3-one) + NADPH + H+ => zymosterol (cholesta-8(9),24-dien-3beta-ol) + NADP+
Zymosterol => cholesta-7,24-dien-3beta-ol
Zymosterol is isomerized to cholesta-7,24-dien-3beta-ol
Cholesta-7,24-dien-3beta-ol is desaturated to form cholesta-5,7,24-trien-3beta-ol
cholesta-7,24-dien-3beta-ol + NADPH + H+ + O2 => cholesta-5,7,24-trien-3beta-ol + NADP+ + 2 H2O
Cholesta-5,7,24-trien-3beta-ol is reduced to desmosterol
cholesta-5,7,24-trien-3beta-ol + NADPH + H+ => desmosterol + NADP+
Reduction of desmosterol to cholesterol
desmosterol + NADPH + H+ => cholesterol + NADP+
Cholesterol biosynthesis