PRKG2
PRKGR2
UniProt:Q13237 PRKG2
UniProt:Q8IVQ6 ZDHHC21
ZDHHC21
N-myristoylation of eNOS
palmitoylation of eNOS
eNOS translocation from Golgi to Caveolae
eNOS associates with Caveolin-1
eNOS:Caveolin-1 complex binds to CaM
HSP90 binds eNOS:Caveolin-1:CaM complex
Caveolin-1 dissociates from eNOS:CaM:HSP90 complex
AKT1 binds eNOS complex via HSP90
AKT1 phosphorylates eNOS
The cofactor BH4 is required for electron transfer in the eNOS catalytic cycle
NO biosynthesis
Uncoupled eNOS favours the formation of superoxide
Superoxide reacts rapidly with NO to form peroxynitrite (ONOO-)
depalmitoylation of eNOS
depalmitoylated eNOS translocates from plasma membrane
eNOS binds NOSIP
eNOS:NOSIP translocation from caveolae to intracellular compartments
eNOS:Caveolin-1 complex binds to Nostrin
eNOS:Caveolin-1:NOSTRIN complex binds dynamin-2
eNOS:Caveolin-1:NOSTRIN:dynamin-2 complex binds N-WASP
NOSTRIN mediated translocation of eNOS from plasma membrane
GCH1 reduces GTP to dihydroneopterin triphosphate
PTPS is phosphorylated by cGMP-dependant protein kinase II
DHNTP is dephosphorylated by PTPS to PTHP
Sepiapterin reductase (SPR) is phosphorylated by Ca2+/calmodulin-dependent protein kinase II
PTHP is reduced to BH4 by sepiapterin reductase (SPR)
BH4 is oxidised to the BH3 radical during the eNOS catalytic cycle
Peroxynitrite can oxidise BH4 to the BH3 radical
Ferrous iron reduces the BH3 radical back to BH4
Ascorbate can reduce the BH3 radical back to BH4
The BH3 radical can decay to dihydrobiopterin (BH2)
BH2 binding can lead to eNOS uncoupling
GCHFR binds to GCH1 and negatively regulates its activity
Salvage - Sepiapterin is reduced to BH2
Salvage - BH2 is reduced to BH4 by DHFR
Metabolism of nitric oxide
eNOS activation and regulation
eNOS activation
NOSIP mediated eNOS trafficking
NOSTRIN mediated eNOS trafficking
Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation