PRKG2
label: PRKGR2
label: UniProt:Q13237 PRKG2

UniProt:Q8IVQ6 ZDHHC21
label: ZDHHC21

N-myristoylation of eNOS
type: GO_0003674
BFO_0000050: eNOS activation
directly activates: palmitoylation of eNOS
directly activates: Catalysis2584

palmitoylation of eNOS
type: Restriction
  on property: BFO_0000066
  some values from: GO_0000139
type: Restriction
  on property: BFO_0000066
  some values from: GO_0000139
type: Restriction
  on property: BFO_0000066
  some values from: GO_0000139
type: Restriction
  on property: enabled by
  some values from: UniProt:Q8IVQ6 ZDHHC21
type: Restriction
  on property: enabled by
  some values from: UniProt:Q8IVQ6 ZDHHC21
type: Restriction
  on property: BFO_0000066
  some values from: GO_0000139
type: Restriction
  on property: enabled by
  some values from: UniProt:Q8IVQ6 ZDHHC21
type: Restriction
  on property: enabled by
  some values from: UniProt:Q8IVQ6 ZDHHC21
type: GO_0016409
BFO_0000050: eNOS activation
directly activates: eNOS translocation from Golgi to Caveolae

eNOS translocation from Golgi to Caveolae
type: GO_0003674
BFO_0000050: eNOS activation
directly activates: eNOS associates with Caveolin-1
directly activates: depalmitoylation of eNOS
directly activates: Catalysis2587

eNOS associates with Caveolin-1
type: GO_0003674
BFO_0000050: eNOS activation
directly activates: eNOS:Caveolin-1 complex binds to CaM
directly activates: eNOS:Caveolin-1 complex binds to Nostrin

eNOS:Caveolin-1 complex binds to CaM
type: GO_0003674
BFO_0000050: eNOS activation
directly activates: HSP90 binds eNOS:Caveolin-1:CaM complex

HSP90 binds eNOS:Caveolin-1:CaM complex
type: GO_0003674
BFO_0000050: eNOS activation
directly activates: Caveolin-1 dissociates from eNOS:CaM:HSP90 complex

Caveolin-1 dissociates from eNOS:CaM:HSP90 complex
type: GO_0003674
BFO_0000050: eNOS activation
directly activates: AKT1 binds eNOS complex via HSP90

AKT1 binds eNOS complex via HSP90
type: GO_0003674
BFO_0000050: eNOS activation
directly activates: AKT1 phosphorylates eNOS

AKT1 phosphorylates eNOS
type: GO_0003674
BFO_0000050: eNOS activation
directly activates: NO biosynthesis
directly activates: Catalysis2585

The cofactor BH4 is required for electron transfer in the eNOS catalytic cycle
type: GO_0003674
BFO_0000050: eNOS activation
directly activates: NO biosynthesis
directly activates: Catalysis2585

NO biosynthesis
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005886
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005886
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005886
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005886
type: GO_0004517
BFO_0000050: eNOS activation

Uncoupled eNOS favours the formation of superoxide
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005886
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005886
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005886
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005886
type: GO_0016175
BFO_0000050: eNOS activation
directly activates: Superoxide reacts rapidly with NO to form peroxynitrite (ONOO-)

Superoxide reacts rapidly with NO to form peroxynitrite (ONOO-)
type: GO_0003674
BFO_0000050: eNOS activation
directly activates: Peroxynitrite can oxidise BH4 to the BH3 radical

depalmitoylation of eNOS
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: GO_0008474
BFO_0000050: eNOS activation
directly activates: depalmitoylated eNOS translocates from plasma membrane

depalmitoylated eNOS translocates from plasma membrane
type: GO_0003674
BFO_0000050: eNOS activation
directly activates: palmitoylation of eNOS
directly activates: AKT1 binds eNOS complex via HSP90
directly activates: Catalysis2584

eNOS binds NOSIP
type: GO_0003674
BFO_0000050: NOSIP mediated eNOS trafficking
directly activates: eNOS:NOSIP translocation from caveolae to intracellular compartments

eNOS:NOSIP translocation from caveolae to intracellular compartments
type: GO_0003674
BFO_0000050: NOSIP mediated eNOS trafficking

eNOS:Caveolin-1 complex binds to Nostrin
type: GO_0003674
BFO_0000050: NOSTRIN mediated eNOS trafficking
directly activates: eNOS:Caveolin-1:NOSTRIN complex binds dynamin-2

eNOS:Caveolin-1:NOSTRIN complex binds dynamin-2
type: GO_0003674
BFO_0000050: NOSTRIN mediated eNOS trafficking
directly activates: eNOS:Caveolin-1:NOSTRIN:dynamin-2 complex binds N-WASP

eNOS:Caveolin-1:NOSTRIN:dynamin-2 complex binds N-WASP
type: GO_0003674
BFO_0000050: NOSTRIN mediated eNOS trafficking
directly activates: NOSTRIN mediated translocation of eNOS from plasma membrane

NOSTRIN mediated translocation of eNOS from plasma membrane
type: GO_0003674
BFO_0000050: NOSTRIN mediated eNOS trafficking

GCH1 reduces GTP to dihydroneopterin triphosphate
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: GO_0003934
BFO_0000050: Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
directly activates: DHNTP is dephosphorylated by PTPS to PTHP
directly activates: Catalysis2590

PTPS is phosphorylated by cGMP-dependant protein kinase II
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: enabled by
  some values from: PRKG2
type: Restriction
  on property: enabled by
  some values from: PRKG2
type: Restriction
  on property: enabled by
  some values from: PRKG2
type: Restriction
  on property: enabled by
  some values from: PRKG2
type: GO_0004692
BFO_0000050: Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
directly activates: DHNTP is dephosphorylated by PTPS to PTHP
directly activates: Catalysis2590

DHNTP is dephosphorylated by PTPS to PTHP
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: GO_0003874
BFO_0000050: Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
directly activates: PTHP is reduced to BH4 by sepiapterin reductase (SPR)
directly activates: Catalysis2592

Sepiapterin reductase (SPR) is phosphorylated by Ca2+/calmodulin-dependent protein kinase II
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: enabled by
  some values from: PRKG2
type: Restriction
  on property: enabled by
  some values from: PRKG2
type: Restriction
  on property: enabled by
  some values from: PRKG2
type: Restriction
  on property: enabled by
  some values from: PRKG2
type: GO_0004692
BFO_0000050: Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
directly activates: PTHP is reduced to BH4 by sepiapterin reductase (SPR)
directly activates: Catalysis2592

PTHP is reduced to BH4 by sepiapterin reductase (SPR)
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: GO_0004757
BFO_0000050: Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
directly activates: BH4 is oxidised to the BH3 radical during the eNOS catalytic cycle
directly activates: Peroxynitrite can oxidise BH4 to the BH3 radical

BH4 is oxidised to the BH3 radical during the eNOS catalytic cycle
type: GO_0003674
BFO_0000050: Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
directly activates: Ferrous iron reduces the BH3 radical back to BH4
directly activates: Ascorbate can reduce the BH3 radical back to BH4
directly activates: The BH3 radical can decay to dihydrobiopterin (BH2)
directly activates: Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation

Peroxynitrite can oxidise BH4 to the BH3 radical
type: GO_0003674
BFO_0000050: Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation

Ferrous iron reduces the BH3 radical back to BH4
type: GO_0003674
BFO_0000050: Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
directly activates: PTHP is reduced to BH4 by sepiapterin reductase (SPR)
directly activates: BH4 is oxidised to the BH3 radical during the eNOS catalytic cycle
directly activates: Peroxynitrite can oxidise BH4 to the BH3 radical
directly activates: Catalysis2592

Ascorbate can reduce the BH3 radical back to BH4
type: GO_0003674
BFO_0000050: Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
directly activates: BH4 is oxidised to the BH3 radical during the eNOS catalytic cycle
directly activates: Peroxynitrite can oxidise BH4 to the BH3 radical

The BH3 radical can decay to dihydrobiopterin (BH2)
type: GO_0003674
BFO_0000050: Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
directly activates: BH2 binding can lead to eNOS uncoupling

BH2 binding can lead to eNOS uncoupling
type: GO_0003674
BFO_0000050: Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
directly activates: Uncoupled eNOS favours the formation of superoxide
directly activates: Catalysis2586

GCHFR binds to GCH1 and negatively regulates its activity
type: GO_0003674
BFO_0000050: Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation

Salvage - Sepiapterin is reduced to BH2
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: GO_0004757
BFO_0000050: Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
directly activates: Salvage - BH2 is reduced to BH4 by DHFR
directly activates: Catalysis2594

Salvage - BH2 is reduced to BH4 by DHFR
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: Restriction
  on property: BFO_0000066
  some values from: GO_0005829
type: GO_0004146
BFO_0000050: Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation

Metabolism of nitric oxide
type: GO_0008150

eNOS activation and regulation
type: GO_0008150
BFO_0000050: Metabolism of nitric oxide

eNOS activation
type: GO_0008150
BFO_0000050: eNOS activation and regulation

NOSIP mediated eNOS trafficking
type: GO_0008150
BFO_0000050: eNOS activation and regulation

NOSTRIN mediated eNOS trafficking
type: GO_0008150
BFO_0000050: eNOS activation and regulation

Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
type: GO_0008150
BFO_0000050: eNOS activation and regulation