Mad1 binds kinetochore
MAD2 associates with the Mad1 kinetochore complex
MAD2 converted to an inhibitory state via interaction with Mad1
Release of activated MAD2 from kinetochores
Inactivation of APC/C via CDC20 sequestration
Formation of the MCC complex
Binding of the MCC complex to the APC/C complex
Mitotic Spindle Checkpoint
Amplification of signal from the kinetochores
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components
Inactivation of APC/C via direct inhibition of the APC/C complex