Mad1 binds kinetochore
type: GO_0003674
BFO_0000050: Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
directly activates: MAD2 associates with the Mad1 kinetochore complex

MAD2 associates with the Mad1 kinetochore complex
type: GO_0003674
BFO_0000050: Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
directly activates: MAD2 converted to an inhibitory state via interaction with Mad1

MAD2 converted to an inhibitory state via interaction with Mad1
type: GO_0003674
BFO_0000050: Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
directly activates: Release of activated MAD2 from kinetochores

Release of activated MAD2 from kinetochores
type: GO_0003674
BFO_0000050: Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
directly activates: Inactivation of APC/C via CDC20 sequestration
directly activates: Formation of the MCC complex
directly activates: Inactivation of APC/C via direct inhibition of the APC/C complex

Inactivation of APC/C via CDC20 sequestration
type: GO_0003674
BFO_0000050: Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components

Formation of the MCC complex
type: GO_0003674
BFO_0000050: Inactivation of APC/C via direct inhibition of the APC/C complex
directly activates: Binding of the MCC complex to the APC/C complex

Binding of the MCC complex to the APC/C complex
type: GO_0003674
BFO_0000050: Inactivation of APC/C via direct inhibition of the APC/C complex
directly activates: BiochemicalReaction2028
directly activates: BiochemicalReaction2520
directly activates: BiochemicalReaction3794

Mitotic Spindle Checkpoint
type: GO_0008150

Amplification of signal from the kinetochores
type: GO_0008150
BFO_0000050: Mitotic Spindle Checkpoint

Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
type: GO_0008150
BFO_0000050: Amplification of signal from the kinetochores

Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components
type: GO_0008150
BFO_0000050: Mitotic Spindle Checkpoint

Inactivation of APC/C via direct inhibition of the APC/C complex
type: GO_0008150
BFO_0000050: Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components